Gutierrez, Rodrigo A. (Santiago, CL)
Nero, Damion C. (Woodside, NY, US)
1. A transgenic plant that ectopically expresses one or more nitrogen-responsive master regulatory genes and exhibits increased nitrogen-assimilation capacity.
2. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes myb family transcription factor (At1g74840).
3. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes CCA1 (At2g46830).
4. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes GLK1 (At2g20570).
5. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes MYB73 (At4g37260).
6. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes bZIP9 transcription factor family protein (At5g24800).
7. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes bZIP1 family transcription factor (At5g49450).
8. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes proline-rich family protein (At5g14540).
9. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes zinc finger (C3HC4-type RING finger) family protein (At5g48655).
10. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes TGA3 (At1g22070).
11. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes proline-rich family protein (At3g01560).
12. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes HB-6 (At2g22430).
13. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes GLK2 (At5g44190).
14. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes RAP2.6 (At1g43160).
15. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes HB-12 (At3g61890).
16. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes AP2 domain-containing transcription factor family protein similar to RAP2.6 (At5g61890).
17. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes homeobox-leucine zipper family protein (At3g61150).
18. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes WRKY family transcription factor (At2g38470).
19. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes ERF1 (At4g17500).
20. The transgenic plant of claim 1, wherein said one or more nitrogen-responsive master regulatory genes comprises a polynucleotide that encodes WRKY family transcription factor (At2g25000).
21. The transgenic plant of claim 1, wherein the plant is species of woody, ornamental, decorative, crop, cereal, fruit, or vegetable.
22. The transgenic plant of claim 1, wherein said plant is a species of one of the following genuses: Acorus, Aegilops, Allium, Amborella, Antirrhinum, Apium, Arabidopsis, Arachis, Beta, Betula, Brassica, Capsicum, Ceratopteris, Citrus, Cryptomeria, Cycas, Descurainia, Eschscholzia, Eucalyptus, Glycine, Gossypium, Hedyotis, Helianthus, Hordeum, Ipomoea, Lactuca, Linum, Liriodendron, Lotus, Lupinus, Lycopersicon, Medicago, Mesembryanthemum, Nicotiana, Nuphar, Pennisetum, Persea, Phaseolus, Physcomitrella, Picea, Pinus, Poncirus, Populus, Prunus, Robinia, Rosa, Saccharum, Schedonorus, Secale, Sesamum, Solanum, Sorghum, Stevia, Thellungiella, Theobroma, Triphysaria, Triticum, Vitis, Zea, or Zinnia.
23. A transgenic plant-derived commercial product, which is derived from a transgenic plant according to claim 1, wherein said product comprises said construct.
24. The transgenic plant-derived commercial product of claim 23, wherein said transgenic plant is a tree, and said commercial product is pulp, paper, a paper product, or lumber.
25. The transgenic plant-derived commercial product of claim 23, wherein said transgenic plant is tobacco, and said commercial product is a cigarette, cigar, or chewing tobacco.
26. The transgenic plant-derived commercial product of claim 23, wherein said transgenic plant is a crop, and said commercial product is a fruit or vegetable.
27. The transgenic plant-derived commercial product of claim 23, wherein said transgenic plant is a grain, and said commercial product is bread, flour, cereal, oat meal, or rice.
28. The transgenic plant-derived commercial product of claim 23, wherein said commercial product is a biofuel or plant oil.
This application is a continuation of U.S. patent application Ser. No. 12/079,001, filed Mar. 24, 2008, which claims the benefit of U.S. Provisional Application No. 60/919,818 filed Mar. 23, 2007, each of which is hereby incorporated by reference herein in its entirety.
1. INTRODUCTION
Provided herein are compositions and methods for affecting nitrogen assimilation, usage and storage by overexpressing or underexpressing certain genes in plants. Such genes include nitrogen-responsive master regulatory control genes CCA1, GLK1 and bZIP1. In some embodiments, provided herein are compositions and methods for genetically engineering plants to increase CCA1 or GLK1 expression or decrease bZIP1 expression, or any combination thereof. In other embodiments, provided herein are compositions and methods for genetically engineering plants to decrease CCA1 or GLK1 expression or increase bZIP1 expression, or any combination thereof. In certain embodiments, the overexpression or underexpression is in a tissue- or cell-specific manner, e.g., in vegetative tissue or in leaves or in fruit or seeds, or in specific cell types (e.g., mesophyll, phloem, etc. In certain specific embodiments, a plant or tree is genetically engineered to increase or constitutively express CCA1 and GLK1 and to decrease expression of bZIP1 in vegetative (growing) tissues of the plant. Such genetically engineered plants are able to assimilate more nitrogen into Gln, such that more nitrogen is available for biosynthesis, and thereby grow larger, more efficiently or rapidly, and/or have increased biomass. Alternatively, the engineered plants may be used to achieve faster growing or maturing crops or, higher crop yields and/or more nutritious products. In certain embodiments, the engineered plants and methods thereof are used in the production of commercial products. Some non-limiting example include genetically engineered trees for e.g., the production of pulp, paper, paper products or lumber; tobacco, e.g., for the production of cigarettes, cigars, or chewing tobacco; crops, e.g., for the production of fruits, vegetables and other food, including grains, e.g., for the production of wheat, bread, flour, rice, corn; and soybean, canola, e.g., for the production of oils or biofuels. In other specific embodiments, a plant or tree is genetically engineered to increase or constitutively express bZIP1 and to decrease expression of CCA1 and GLK1 in leaves, fruit and/or seed tissues of the plant. This would serve to increase N-assimilation into Asn, an inert N-storage compound used to transport N to seed, for example.
2. BACKGROUND
Nitrate is a key required nutrient for the synthesis of amino acids, nucleotides and vitamins and is commonly considered to be the most limiting for normal plant growth (Vitousek et al., 2004, Biogeochemistry 13). Nitrogenous fertilizer is usually supplied as ammonium nitrate, potassium nitrate, or urea. Plants are keenly sensitive to nitrogen levels in the soil and, atypically of animal development, adopt their body plan to cope with their environment (Lopez-Bucio et al., 2003, Curr Opin Plant Biol 6:280-287; Malamy et al., 2005, Plant Cell Environ 28:67-77; Walch-Liu et al., 2006, Ann Bot (Lond) 97:875-81). For example, mutants in several general nitrogen (N)-assimilation genes affect root architecture (Little et al., 2005, Proc Natl Acad Sci USA 102:13693-13698; Remans et al., 2006, Proc Natl Acad Sci USA 103:19206-19211). Transduction of this nitrogen signal is linked to a massive and concerted gene expression response in the root (Gutierrez et al., 2007, Genome Biol 8:R7; Wang et al., 2003, Plant Physiol 132:556-67).
Studies on the regulation of genes involved in the N-assimilatory pathway have shown that genes involved in N-assimilation are regulated transcriptionally by both inorganic and organic forms of nitrogen (FIG. 1). Genes involved in the uptake and reduction of nitrate (NIA, NIR) are transcriptionally induced by nitrate. By contrast, the glutamine synthetase gene (GLN1.3) involved in assimilating inorganic N into organic form (Gln), is transcriptionally repressed by the endproducts of N-assimilation (Glu/Gln) (FIG. 1). The repression of GLN1.3 expression by the product of the GS enzyme reaction serves as a negative feedback loop, that shuts off further assimilation of inorganic N into Gln, when levels of Gln are abundant. As GS is and ATP dependent enzyme, this is likely to be an energy conservation mechanism. By contrast, Gln/Glu levels activate the expression of the ASN1 gene (asparagine synthetase) which serves to transfer the amide N from Gln onto Asp to make Asn and Glu as a by-product. Asn is an inert amino acid used to store N and used for long distance N-transport (e.g., to seed). The induction of ASN1 by Glu/Gln is a mechanism that serves to store excess N as Asn, which is used to transport N to seed.
It would be advantageous to produce plants that would continue to assimilate and utilize N or to store N depending on whether a vegetative plant part or seed is the product, respectively, making N-assimilation independent of the Glu/Gln biofeedback pathway. N-assimilated into Glu/Gln by GS is used in the biosynthesis of all N-containing compounds including essentially all other amino acids, nucleic acids and chlorophyll. By contrast, the conversion of Gln to Asn (an inert N source) is used to transport and store N in seed.
3. SUMMARY
Master control genes (CCA1, GLK1, and bZIP11) that control N-assimilation in response to Glu sensing have been identified in the present invention. As these genes are transcription factor hubs, they coordinate the N-regulation of the N-assimilatory gene network, with genome-wide responses associated with growth and development in plants. Thus, effecting genome-wide changes in N-assimilation, plant growth and development, by the transgenic manipulation of these master control genes in plants will effect nitrogen use efficiency in vegetative tissues (leaves & roots) and also in seed. Changes in levels of N-assimilated into Gln effect changes in growth of vegetative tissues, while changes in levels of Asn affect seed development and nitrogen content.
Thus, the present invention relates to the transgenic manipulation of these N-responsive master regulatory genes (CCA1, GLK1, and bZIP11) that control N-assimilation, and other related processes in response to N treatments, so as to increase the overall N-assimilation capacity, whether for increased N usage or N storage. The overexpression of these master control genes (e.g., uncoupled from Glu repression) effectively releases N-assimilation from the feedback repression loop by Glu-leading to increased N-assimilation and usage. As these regulatory genes serve to respond to Glu levels by reciprocally regulating the amount of N-assimilated into Gln versus the amount of Gln metabolized to Asn (for N-storage and transport), the manipulation of these genes in transgenic plants can be used to optimize N-assimilation into Gln versus Asn (FIG. 2, FIG. 12). Increased N-assimilation is advantageous in all crops. Additionally, in seed crops, the increased synthesis of Asn increases N-transported and stored in seed. The genes, CCA1, GLK1 and/or bZIP1, can be expressed using constitutive promoters. Alternately, developmentally regulated promoters can be used to over express CCA1 and/or GLK1 that favors Gln production early in the life cycle, while switching to overexpressing bZIP1 which favors Asn production later in the life cycle (e.g., during seed set). Further, underexpressing CCA1 and/or GLK1 favors Asn production and underexpressing bZIP1 favors Gln production.
As CCA1/GLK1 and bZIP11 are master regulatory genes controlling genes in the N-assimilatory pathway as well as other processes, the manipulation of these genes in transgenic plants can coordinate an increase in N-assimilation and usage (increased Gln synthesis), and/or an increase in stored N (increased Asn synthesis) with genes controlling aspects of growth and development, that are also targets for these master regulatory genes.
Thus, in one embodiment, the present invention is directed to a method for improving nitrogen assimilation and usage in a plant in which more nitrogen is available for biosynthesis, said method comprising overexpressing GLK1 in the plant. In another embodiment, the present invention is directed to a method for improving nitrogen assimilation and usage in a plant in which more nitrogen is available for biosynthesis, said method comprising overexpressing CCA1 in the plant. In yet another embodiment, the present invention is directed to a method for improving nitrogen assimilation and usage in a plant in which more nitrogen is available for biosynthesis, said method comprising underexpressing bZIP1 in the plant.
In another embodiment, the method for improving nitrogen assimilation and usage in a plant in which more nitrogen is available for biosynthesis comprises overexpressing CCA1 and GLK1 in the plant. In another embodiment, the method for improving nitrogen assimilation and usage in a plant in which more nitrogen is available for biosynthesis comprises overexpressing CCA1 and underexpressing bZIP1 in the plant, or overexpressing GLK1 and underexpressing bZIP1 in the plant. In yet another embodiment, the method for improving nitrogen assimilation and usage in a plant in which more nitrogen is available for biosynthesis comprises overexpressing CCA1, overexpressing GLK1 and underexpressing bZIP1 in the plant.
The present invention is also directed to methods for altering nitrogen assimilation and storage, e.g., increasing nitrogen storage, in a plant. In one embodiment, the method comprises overexpressing bZIP1 in the plant. In another embodiment, the method comprises underexpressing CCA1 in the plant and/or underexpressing GLK1 in the plant. In another embodiment, the method overexpressing bZIP1 and underexpressing CCA1 and/or GLK1 in the plant. In yet another embodiment, the method comprises overexpressing bZIP1, and underexpressing CCA1 and underexpressing GLK1 in the plant.
In certain embodiments, the plant is species of woody, ornamental, decorative, crop, cereal, fruit, or vegetable. In other embodiments, the plant is a species of one of the following genuses: Acorus, Aegilops, Allium, Amborella, Antirrhinum, Apium, Arabidopsis, Arachis, Beta, Betula, Brassica, Capsicum, Ceratopteris, Citrus, Cryptomeria, Cycas, Descurainia, Eschscholzia, Eucalyptus, Glycine, Gossypium, Hedyotis, Helianthus, Hordeum, Ipomoea, Lactuca, Linum, Liriodendron, Lotus, Lupinus, Lycopersicon, Medicago, Mesembryanthemum, Nicotiana, Nuphar, Pennisetum, Persea, Phaseolus, Physcomitrella, Picea, Pinus, Poncirus, Populus, Prunus, Robinia, Rosa, Saccharum, Schedonorus, Secale, Sesamum, Solanum, Sorghum, Stevia, Thellungiella, Theobroma, Triphysaria, Triticum, Vitis, Zea, or Zinnia.
The overexpression of a particular gene can be accomplished by any method known in the art, for example, by transforming a plant cell with a nucleic acid vector comprising the coding sequences of the desired gene operably linked to a promoter active in a plant cell such that the desired gene is expressed at levels higher than normal, i.e., levels found in a control/nontransgenic plant. Such promoters can be constitutively active in all or some plant tissues or can be inducible.
The underexpression of a desired gene can be accomplished by any method known in the art, such as knocking out the gene or mutating the gene transgenically such that lower than normal levels of the gene product is produced in the transgenic cells or plant. For example, such mutations include frame-shift mutations or mutations resulting in a stop codon in the wild-type coding sequence, thus preventing expression of the gene product. Another exemplary mutation would be the removal of the transcribed sequences from the plant genome, for example, by homologous recombination. Another method for underexpressing a gene is transgenically introducing an insertion or deletion into the transcribed sequence or an insertion or deletion upstream or downstream of the transcribed sequence such that expression of the gene product is decreased as compared to wild-type or appropriate control. Additionally, microRNA can be used to target a particular encoding mRNA for degradation, thus reducing the level of the expressed gene product in the transgenic plant cell.
The present invention is also directed to a transgenic plant produced by any of the foregoing methods.
The present invention is also directed to compositions for modulating gene expression in plants. The compositions comprise constructs for the expression of CCA1, GLK1 or bZIP1. In certain embodiments, a construct of the invention comprises a promoter, such as a tissue specific promoter, which is expressed in a plant cell, such as a leaf cell, and promotes the expression of CCA1, GLK1 or bZIP1.
Any of a variety of promoters can be utilized in the constructs of the invention depending on the desired outcome. Tissue-specific or tissue-preferred promoters, inducible promoters, developmental promoters, constitutive promoters and/or chimeric promoters can be used to direct expression of the gene product in specific cells or organs the plant, when fused to the appropriate cell or organ specific promoter.
Chimeric constructs expressing CCA1, GLK1 or bZIP1 in transgenic plants (using constitutive or inducible promoters) can be used in the compositions and methods provided herein to enhance nitrogen assimilation and usage or increase nitrogen storage.
The present invention is also directed to a transgenic plant-derived commercial product. In one embodiment, the transgenic plant is a tree, and said commercial product is pulp, paper, a paper product, or lumber. In another embodiment, the transgenic plant is tobacco, and said commercial product is a cigarette, cigar, or chewing tobacco. In yet another embodiment, the transgenic plant is a crop, and said commercial product is a fruit or vegetable. In yet another embodiment, the transgenic plant is a grain, and said commercial product is bread, flour, cereal, oat meal, or rice. In another embodiment, the product is a biofuel or a plant oil.
4. TERMINOLOGY
Units, prefixes, and symbols may be denoted in their SI accepted form. Unless otherwise indicated, nucleic acids are written left to right in 5′ to 3′ orientation; amino acid sequences are written left to right in amino to carboxyl orientation, respectively. Numeric ranges recited within the specification are inclusive of the numbers defining the range and include each integer within the defined range. Amino acids may be referred to herein by either their commonly known three letter symbols or by the one-letter symbols recommended by the IUPAC-IUB Biochemical Nomenclature Commission. Nucleotides, likewise, may be referred to by their commonly accepted single-letter codes. Unless otherwise provided for, software, electrical, and electronics terms as used herein are as defined in The New IEEE Standard Dictionary of Electrical and Electronics Terms (5th edition, 1993). The terms defined below are more fully defined by reference to the specification as a whole.
As used herein, the term “agronomic” includes, but is not limited to, changes in root size, vegetative yield, seed yield or overall plant growth. Other agronomic properties include factors desirable to agricultural production and business.
By “amplified” is meant the construction of multiple copies of a nucleic acid sequence or multiple copies complementary to the nucleic acid sequence using at least one of the nucleic acid sequences as a template. Amplification systems include the polymerase chain reaction (PCR) system, ligase chain reaction (LCR) system, nucleic acid sequence based amplification (NASBA, Cangene, Mississauga, Ontario), Q-Beta Replicase systems, transcription-based amplification system (TAS), and strand displacement amplification (SDA). See, e.g., Diagnostic Molecular Microbiology: Principles and Applications, D. H. Persing et al., Ed., 1993, American Society for Microbiology, Washington, D.C. The product of amplification is termed an amplicon.
As used herein, “antisense orientation” includes reference to a duplex polynucleotide sequence that is operably linked to a promoter in an orientation where the antisense strand is transcribed. The antisense strand is sufficiently complementary to an endogenous transcription product such that translation of the endogenous transcription product is often inhibited.
In its broadest sense, a “delivery system,” as used herein, is any vehicle capable of facilitating delivery of a nucleic acid (or nucleic acid complex) to a cell and/or uptake of the nucleic acid by the cell.
The term “ectopic” is used herein to mean abnormal subcellular (e.g., switch between organellar and cytosolic localization), cell-type, tissue-type and/or developmental or temporal expression (e.g., light/dark) patterns for the particular gene or enzyme in question. Such ectopic expression does not necessarily exclude expression in tissues or developmental stages normal for said enzyme but rather entails expression in tissues or developmental stages not normal for the said enzyme.
By “endogenous nucleic acid sequence” and similar terms, it is intended that the sequences are natively present in the recipient plant genome and not substantially modified from its original form.
The term “exogenous nucleic acid sequence” as used herein refers to a nucleic acid foreign to the recipient plant host or, native to the host if the native nucleic acid is substantially modified from its original form. For example, the term includes a nucleic acid originating in the host species, where such sequence is operably linked to a promoter that differs from the natural or wild-type promoter.
By “encoding” or “encoded”, with respect to a specified nucleic acid, is meant comprising the information for translation into the specified protein. A nucleic acid encoding a protein may comprise non-translated sequences (e.g., introns) within translated regions of the nucleic acid, or may lack such intervening non-translated sequences (e.g., as in cDNA). The information by which a protein is encoded is specified by the use of codons. Typically, the amino acid sequence is encoded by the nucleic acid using the “universal” genetic code. However, variants of the universal code, such as are present in some plant, animal, and fungal mitochondria, the bacterium Mycoplasma capricolum, or the ciliate Macronucleus, may be used when the nucleic acid is expressed therein.
When the nucleic acid is prepared or altered synthetically, advantage can be taken of known codon preferences of the intended host where the nucleic acid is to be expressed. For example, although nucleic acid sequences of the present invention may be expressed in both monocotyledonous and dicotyledonous plant species, sequences can be modified to account for the specific codon preferences and GC content preferences of monocotyledons or dicotyledons as these preferences have been shown to differ (Murray et al., 1989, Nucl. Acids Res. 17: 477-498). Thus, the maize preferred codon for a particular amino acid may be derived from known gene sequences from maize. Maize codon usage for 28 genes from maize plants is listed in Table 4 of Murray et al., supra.
By “fragment” is intended a portion of the nucleotide sequence. Fragments of the modulator sequence will generally retain the biological activity of the native suppressor protein. Alternatively, fragments of the targeting sequence may or may not retain biological activity. Such targeting sequences may be useful as hybridization probes, as antisense constructs, or as co-suppression sequences. Thus, fragments of a nucleotide sequence may range from at least about 20 nucleotides, about 50 nucleotides, about 100 nucleotides, and up to the full-length nucleotide sequence of the invention.
As used herein “full-length sequence” in reference to a specified polynucleotide or its encoded protein means having the entire amino acid sequence of, a native (non-synthetic), endogenous, biologically active form of the specified protein. Methods to determine whether a sequence is full-length are well known in the art including such exemplary techniques as northern or western blots, primer extension, 51 protection, and ribonuclease protection. See, e.g., Plant Molecular Biology: A Laboratory Manual, Clark, Ed., 1997, Springer-Verlag, Berlin. Comparison to known full-length homologous (orthologous and/or paralogous) sequences can also be used to identify full-length sequences of the present invention. Additionally, consensus sequences typically present at the 5′ and 3′ untranslated regions of mRNA aid in the identification of a polynucleotide as full-length. For example, the consensus sequence ANNNNAUGG, where the underlined codon represents the N-terminal methionine, aids in determining whether the polynucleotide has a complete 5′ end. Consensus sequences at the 3′ end, such as polyadenylation sequences, aid in determining whether the polynucleotide has a complete 3′ end.
The term “gene activity” refers to one or more steps involved in gene expression, including transcription, translation, and the functioning of the protein encoded by the gene.
The term “genetic modification” as used herein refers to the introduction of one or more exogenous nucleic acid sequences, e.g., CCA1, GLK1 or bZIP1 encoding sequences, as well as regulatory sequences, into one or more plant cells, which in certain cases can generate whole, sexually competent, viable plants. The term “genetically modified” or “genetically engineered” as used herein refers to a plant which has been generated through the aforementioned process. Genetically modified plants of the invention are capable of self-pollinating or cross-pollinating with other plants of the same species so that the foreign gene, carried in the germ line, can be inserted into or bred into agriculturally useful plant varieties.
As used herein, “heterologous” in reference to a nucleic acid is a nucleic acid that originates from a foreign species, or, if from the same species, is substantially modified from its native form in composition and/or genomic locus by deliberate human intervention. For example, a promoter operably linked to a heterologous structural gene is from a species different from that from which the structural gene was derived, or, if from the same species, one or both are substantially modified from their original form. A heterologous protein may originate from a foreign species or, if from the same species, is substantially modified from its original form by deliberate human intervention.
By “host cell” is meant a cell that contains a vector and supports the replication and/or expression of the vector. Host cells may be prokaryotic cells such as E. coli, or eukaryotic cells such as yeast, plant, insect, amphibian, or mammalian cells. Preferably, host cells are monocotyledonous or dicotyledonous plant cells. A particularly preferred monocotyledonous host cell is a maize host cell.
The term “introduced” in the context of inserting a nucleic acid into a cell, means “transfection” or “transformation” or “transduction” and includes reference to the incorporation of a nucleic acid into a eukaryotic or prokaryotic cell where the nucleic acid may be incorporated into the genome of the cell (e.g., chromosome, plasmid, plastid or mitochondrial DNA), converted into an autonomous replicon, or transiently expressed (e.g., transfected mRNA).
The term “isolated” refers to material, such as a nucleic acid or a protein, which is: (1) substantially or essentially free from components which normally accompany or interact with it as found in its natural environment. The isolated material optionally comprises material not found with the material in its natural environment; or (2) if the material is in its natural environment, the material has been synthetically altered or synthetically produced by deliberate human intervention and/or placed at a different location within the cell. The synthetic alteration or creation of the material can be performed on the material within or apart from its natural state. For example, a naturally-occurring nucleic acid becomes an isolated nucleic acid if it is altered or produced by non-natural, synthetic methods, or if it is transcribed from DNA which has been altered or produced by non-natural, synthetic methods. See, e.g., Compounds and Methods for Site Directed Mutagenesis in Eukaryotic Cells, Kmiec, U.S. Pat. No. 5,565,350; In vivo Homologous Sequence Targeting in Eukaryotic Cells; Zarling et al., PCT/US93/03868. The isolated nucleic acid may also be produced by the synthetic re-arrangement (“shuffling”) of a part or parts of one or more allelic forms of the gene of interest. Likewise, a naturally-occurring nucleic acid (e.g., a promoter) becomes isolated if it is introduced to a different locus of the genome. Nucleic acids which are “isolated,” as defined herein, are also referred to as “heterologous” nucleic acids.
As used herein, the term “marker” refers to a gene encoding a trait or a phenotype which permits the selection of, or the screening for, a plant or plant cell containing the marker.
As used herein, “nucleic acid” includes reference to a deoxyribonucleotide or ribonucleotide polymer, or chimeras thereof, in either single- or double-stranded form, and unless otherwise limited, encompasses known analogues having the essential nature of natural nucleotides in that they hybridize to single-stranded nucleic acids in a manner similar to naturally occurring nucleotides (e.g., peptide nucleic acids).
By “nucleic acid library” is meant a collection of isolated DNA or RNA molecules which comprise and substantially represent the entire transcribed fraction of a genome of a specified organism or of a tissue from that organism. Construction of exemplary nucleic acid libraries, such as genomic and cDNA libraries, is taught in standard molecular biology references such as Berger and Kimmel, Guide to Molecular Cloning Techniques, Methods in Enzymology, Vol. 152, Academic Press, Inc., San Diego, Calif. (Berger); Sambrook et al., 1989, Molecular Cloning—A Laboratory Manual, 2nd ed., Vol. 1-3; and Current Protocols in Molecular Biology, F. M. Ausubel et al., Eds., 1994, Current Protocols, a joint venture between Greene Publishing Associates, Inc. and John Wiley & Sons, Inc.
As used herein “operably linked” includes reference to a functional linkage between a promoter and a second sequence, wherein the promoter sequence initiates and mediates transcription of the DNA sequence corresponding to the second sequence. Generally, operably linked means that the nucleic acid sequences being linked are contiguous and, where necessary to join two protein coding regions, contiguous and in the same reading frame.
He term “orthologous” as used herein describes a relationship between two or more polynucleotides or proteins. Two polynucleotides or proteins are “orthologous” to one another if they are derived from a common ancestral gene and serve a similar function in different organisms. In general, orthologous polynucleotides or proteins will have similar catalytic functions (when they encode enzymes) or will serve similar structural functions (when they encode proteins or RNA that form part of the ultrastructure of a cell).
The term “overexpression” is used herein to mean above the normal expression level in the particular tissue, all and/or developmental or temporal stage for said enzyme/expressed protein product.
As used herein, the term “plant” is used in its broadest sense, including, but is not limited to, any species of woody, ornamental or decorative, crop or cereal, fruit or vegetable plant, and algae (e.g., Chlamydomonas reinhardtii). Non-limiting examples of plants include plants from the genus Arabidopsis or the genus Oryza. Other examples include plants from the genuses Acorus, Aegilops, Allium, Amborella, Antirrhinum, Apium, Arachis, Beta, Betula, Brassica, Capsicum, Ceratopteris, Citrus, Cryptomeria, Cycas, Descurainia, Eschscholzia, Eucalyptus, Glycine, Gossypium, Hedyotis, Helianthus, Hordeum, Ipomoea, Lactuca, Linum, Liriodendron, Lotus, Lupinus, Lycopersicon, Medicago, Mesembryanthemum, Nicotiana, Nuphar, Pennisetum, Persea, Phaseolus, Physcomitrella, Picea, Pinus, Poncirus, Populus, Prunus, Robinia, Rosa, Saccharum, Schedonorus, Secale, Sesamum, Solanum, Sorghum, Stevia, Thellungiella, Theobroma, Triphysaria, Triticum, Vitis, Zea, or Zinnia.” Plants included in the invention are any plants amenable to transformation techniques, including gymnosperms and angiosperms, both monocotyledons and dicotyledons. Examples of monocotyledonous angiosperms include, but are not limited to, asparagus, field and sweet corn, barley, wheat, rice, sorghum, onion, pearl millet, rye and oats and other cereal grains. Examples of dicotyledonous angiosperms include, but are not limited to tomato, tobacco, cotton, rapeseed, field beans, soybeans, peppers, lettuce, peas, alfalfa, clover, cole crops or Brassica oleracea (e.g., cabbage, broccoli, cauliflower, brussel sprouts), radish, carrot, beets, eggplant, spinach, cucumber, squash, melons, cantaloupe, sunflowers and various ornamentals. Examples of woody species include poplar, pine, sequoia, cedar, oak, etc. Still other examples of plants include, but are not limited to, wheat, cauliflower, tomato, tobacco, corn, petunia, trees, etc. As used herein, the term “cereal crop” is used in its broadest sense. The term includes, but is not limited to, any species of grass, or grain plant (e.g., barley, corn, oats, rice, wild rice, rye, wheat, millet, sorghum, triticale, etc.), non-grass plants (e.g., buckwheat flax, legumes or soybeans, etc.). As used herein, the term “crop” or “crop plant” is used in its broadest sense. The term includes, but is not limited to, any species of plant or algae edible by humans or used as a feed for animals or used, or consumed by humans, or any plant or algae used in industry or commerce. As used herein, the term “plant” also refers to either a whole plant, a plant part, or organs (e.g., leaves, stems, roots, etc.), a plant cell, or a group of plant cells, such as plant tissue, plant seeds and progeny of same. Plantlets are also included within the meaning of “plant.” The class of plants which can be used in the methods of the invention is generally as broad as the class of higher plants amenable to transformation techniques, including both monocotyledonous and dicotyledonous plants.
The term “plant cell” as used herein refers to protoplasts, gamete producing cells, and cells which regenerate into whole plants. Plant cell, as used herein, further includes, without limitation, cells obtained from or found in: seeds, suspension cultures, embryos, meristematic regions, callus tissue, leaves, roots, shoots, gametophytes, sporophytes, pollen, and microspores. Plant cells can also be understood to include modified cells, such as protoplasts, obtained from the aforementioned tissues.
As used herein, “polynucleotide” includes reference to a deoxyribopolynucleotide, ribopolynucleotide, or chimeras or analogs thereof that have the essential nature of a natural deoxy- or ribo-nucleotide in that they hybridize, under stringent hybridization conditions, to substantially the same nucleotide sequence as naturally occurring nucleotides and/or allow translation into the same amino acid(s) as the naturally occurring nucleotide(s). A polynucleotide can be full-length or a subsequence of a native or heterologous structural or regulatory gene. Unless otherwise indicated, the term includes reference to the specified sequence as well as the complementary sequence thereof. Thus, DNAs or RNAs with backbones modified for stability or for other reasons are “polynucleotides” as that term is intended herein. Moreover, DNAs or RNAs comprising unusual bases, such as inosine, or modified bases, such as tritylated bases, to name just two examples, are polynucleotides as the term is used herein. It will be appreciated that a great variety of modifications have been made to DNA and RNA that serve many useful purposes known to those of skill in the art. The term polynucleotide as it is employed herein embraces such chemically-, enzymatically- or metabolically-modified forms of polynucleotides, as well as the chemical forms of DNA and RNA characteristic of viruses and cells, including among other things, simple and complex cells.
The terms “polypeptide”, “peptide” and “protein” are used interchangeably herein to refer to a polymer of amino acid residues. The terms apply to amino acid polymers in which one or more amino acid residue is an artificial chemical analogue of a corresponding naturally-occurring amino acid, as well as to naturally-occurring amino acid polymers. The essential nature of such analogues of naturally-occurring amino acids is that, when incorporated into a protein, that protein is specifically reactive to antibodies elicited to the same protein but consisting entirely of naturally occurring amino acids. The terms “polypeptide”, “peptide” and “protein” are also inclusive of modifications including, but not limited to, glycosylation, lipid attachment, sulfation, gamma-carboxylation of glutamic acid residues, hydroxylation and ADP-ribosylation. Further, this invention contemplates the use of both the methionine-containing and the methionine-less amino terminal variants of the protein of the invention.
As used herein “promoter” includes reference to a region of DNA upstream from the start of transcription and involved in recognition and binding of RNA polymerase and other proteins to initiate transcription. A “plant promoter” is a promoter capable of initiating transcription in plant cells whether or not its origin is a plant cell. Exemplary plant promoters include, but are not limited to, those that are obtained from plants, plant viruses, and bacteria which comprise genes expressed in plant cells such Agrobacterium or Rhizobium. Examples of promoters under developmental control include promoters that preferentially initiate transcription in certain tissues, such as leaves, roots, or seeds. Such promoters are referred to as “tissue preferred.” Promoters which initiate transcription only in certain tissue are referred to as “tissue specific.” A “cell type” specific promoter primarily drives expression in certain cell types in one or more organs, for example, vascular cells in roots or leaves. An “inducible” or “repressible” promoter is a promoter which is under environmental control. Examples of environmental conditions that may effect transcription by inducible promoters include anaerobic conditions or the presence of light. Tissue specific, tissue preferred, cell type specific, and inducible promoters represent the class of “non-constitutive” promoters. A “constitutive” promoter is a promoter which is active under most environmental conditions.
As used herein “recombinant” includes reference to a cell or vector that has been modified by the introduction of a heterologous nucleic acid, or to a cell derived from a cell so modified. Thus, for example, recombinant cells express genes that are not found in identical form within the native (non-recombinant) form of the cell, or exhibit altered expression of native genes, as a result of deliberate human intervention. The term “recombinant” as used herein does not encompass the alteration of the cell or vector by events (e.g., spontaneous mutation, natural transformation, transduction, or transposition) occurring without deliberate human intervention.
As used herein, a “recombinant expression cassette” is a nucleic acid construct, generated recombinantly or synthetically, with a series of specified nucleic acid elements which permit transcription of a particular nucleic acid in a host cell. The recombinant expression cassette can be incorporated into a plasmid, chromosome, mitochondrial DNA, plastid DNA, virus, or nucleic acid fragment. Typically, the recombinant expression cassette portion of an expression vector includes, among other sequences, a nucleic acid to be transcribed, and a promoter.
The term “regulatory sequence” as used herein refers to a nucleic acid sequence capable of controlling the transcription of an operably associated gene. Therefore, placing a gene under the regulatory control of a promoter or a regulatory element means positioning the gene such that the expression of the gene is controlled by the regulatory sequence(s). Because a microRNA binds to its target, it is a post transcriptional mechanism for regulating levels of mRNA. Thus, an miRNA can also be considered a “regulatory sequence” herein. Not just transcription factors.
The term “residue” or “amino acid residue” or “amino acid” are used interchangeably herein to refer to an amino acid that is incorporated into a protein, polypeptide, or peptide (collectively “protein”). The amino acid may be a naturally occurring amino acid and, unless otherwise limited, may encompass non-natural analogs of natural amino acids that can function in a similar manner as naturally occurring amino acids.
The term “tissue-specific promotor” is a polynucleotide sequence that specifically binds to transcription factors expressed primarily or only in such specific tissue.
The term “selectively hybridizes” includes reference to hybridization, under stringent hybridization conditions, of a nucleic acid sequence to a specified nucleic acid target sequence to a detectably greater degree (e.g., at least 2-fold over background) than its hybridization to non-target nucleic acid sequences and to the substantial exclusion of non-target nucleic acids. Selectively hybridizing sequences typically have about at least 80% sequence identity, preferably 90% sequence identity, and most preferably 100% sequence identity (i.e., complementary) with each other.
As used herein, a “stem-loop motif” or a “stem-loop structure,” sometimes also referred to as a “hairpin structure,” is given its ordinary meaning in the art, i.e., in reference to a single nucleic acid molecule having a secondary structure that includes a double-stranded region (a “stem” portion) composed of two regions of nucleotides (of the same molecule) forming either side of the double-stranded portion, and at least one “loop” region, comprising uncomplemented nucleotides (i.e., a single-stranded region).
The term “stringent conditions” or “stringent hybridization conditions” includes reference to conditions under which a probe will selectively hybridize to its target sequence, to a detectably greater degree than to other sequences (e.g., at least 2-fold over background). Stringent conditions are sequence-dependent and will be different in different circumstances. By controlling the stringency of the hybridization and/or washing conditions, target sequences can be identified which are 100% complementary to the probe (homologous probing). Alternatively, stringency conditions can be adjusted to allow some mismatching in sequences so that lower degrees of similarity are detected (heterologous probing). Generally, a probe is less than about 1000 nucleotides in length, optionally less than 500 nucleotides in length.
Typically, stringent conditions will be those in which the salt concentration is less than about 1.5 M Na ion, typically about 0.01 to 1.0 M Na ion concentration (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30° C. for short probes (e.g., 10 to 50 nucleotides) and at least about 60° C. for long probes (e.g., greater than 50 nucleotides). Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide. Exemplary low stringency conditions include hybridization with a buffer solution of 30 to 35% formamide, 1 M NaCl, 1% SDS (sodium dodecyl sulphate) at 37° C., and a wash in 1× to 2×SSC (20×SSC=3.0 M NaCl/0.3 M trisodium citrate) at 50 to 55° C. Exemplary moderate stringency conditions include hybridization in 40 to 45% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 0.5× to 1×SSC at 55 to 60° C. Exemplary high stringency conditions include hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 0.1×SSC at 60 to 65° C.
Specificity is typically the function of post-hybridization washes, the critical factors being the ionic strength and temperature of the final wash solution. For DNA-DNA hybrids, the Tm can be approximated from the equation of Meinkoth and Wahl, 1984, Anal. Biochem., 138:267-284: Tm=81.5° C.+16.6 (log M)+0.41 (% GC)−0.61 (% form)−500/L; where M is the molarity of monovalent cations, % GC is the percentage of guanosine and cytosine nucleotides in the DNA, % form is the percentage of formamide in the hybridization solution, and L is the length of the hybrid in base pairs. The Tm is the temperature (under defined ionic strength and pH) at which 50% of a complementary target sequence hybridizes to a perfectly matched probe. Tm is reduced by about 1° C. for each 1% of mismatching; thus, Tm, hybridization and/or wash conditions can be adjusted to hybridize to sequences of the desired identity. For example, if sequences with ≧90% identity are sought, the Tm can be decreased 10° C. Generally, stringent conditions are selected to be about 5° C. lower than the thermal melting point (Tm) for the specific sequence and its complement at a defined ionic strength and pH. However, severely stringent conditions can utilize a hybridization and/or wash at 1, 2, 3, or 4° C. lower than the thermal melting point (Tm); moderately stringent conditions can utilize a hybridization and/or wash at 6, 7, 8, 9, or 10° C. lower than the thermal melting point (Tm); low stringency conditions can utilize a hybridization and/or wash at 11, 12, 13, 14, 15, or 20° C. lower than the thermal melting point (Tm). Using the equation, hybridization and wash compositions, and desired Tm, those of ordinary skill will understand that variations in the stringency of hybridization and/or wash solutions are inherently described. If the desired degree of mismatching results in a Tm of less than 45° C. (aqueous solution) or 32° C. (formamide solution) it is preferred to increase the SSC concentration so that a higher temperature can be used. An extensive guide to the hybridization of nucleic acids is found in Tijssen, 1993, Laboratory Techniques in Biochemistry and Molecular Biology—Hybridization with Nucleic Acid Probes, Part I, Chapter 2 “Overview of principles of hybridization and the strategy of nucleic acid probe assays”, Elsevier, New York; and Current Protocols in Molecular Biology, Chapter 2, Ausubel et al., Eds., 1995, Greene Publishing and Wiley-Interscience, New York. Hybridization and/or wash conditions can be applied for at least 10, 30, 60, 90, 120, or 240 minutes.
As used herein, “transcription factor” includes reference to a protein which interacts with a DNA regulatory element to affect expression of a structural gene or expression of a second regulatory gene. “Transcription factor” may also refer to the DNA encoding said transcription factor protein. The function of a transcription factor may include activation or repression of transcription initiation.
The term “transfection,” as used herein, refers to the introduction of a nucleic acid into a cell, for example, a nucleotide sequence able to be transcribed to produce CCA1, GLK1 or BZIP1 protein.
As used herein, the term “transformation” means alteration of the genotype of a host plant by the introduction of a CCA1, GLK1 or BZIP1 nucleic acid sequence.
As used herein, “transgenic plant” includes reference to a plant which comprises within its genome a heterologous polynucleotide. Generally, the heterologous polynucleotide is stably integrated within the genome such that the polynucleotide is passed on to successive generations. The heterologous polynucleotide may be integrated into the genome alone or as part of a recombinant expression cassette. “Transgenic” is used herein to include any cell, cell line, callus, tissue, plant part or plant, the genotype of which has been altered by the presence of heterologous nucleic acid including those transgenics initially so altered as well as those created by sexual crosses or asexual propagation from the initial transgenic. The term “transgenic” as used herein does not encompass the alteration of the genome (chromosomal or extra-chromosomal) by conventional plant breeding methods or by naturally occurring events such as random cross-fertilization, non-recombinant viral infection, non-recombinant bacterial transformation, non-recombinant transposition, or spontaneous mutation.
The term “underexpression” is used herein to mean below the normal expression level in the particular tissue, all and/or developmental or temporal stage for said enzyme/expressed protein product.
As used herein, “vector” includes reference to a nucleic acid used in introduction of a polynucleotide of the present invention into a host cell. Vectors are often replicons. Expression vectors permit transcription of a nucleic acid inserted therein.
The following terms are used to describe the sequence relationships between a polynucleotide/polypeptide of the present invention with a reference polynucleotide/polypeptide: (a) “reference sequence”, (b) “comparison window”, (c) “sequence identity”, and (d) “percentage of sequence identity”.
(a) As used herein, “reference sequence” is a defined sequence used as a basis for sequence comparison with a polynucleotide/polypeptide of the present invention. A reference sequence may be a subset or the entirety of a specified sequence; for example, as a segment of a full-length cDNA or gene sequence, or the complete cDNA or gene sequence.
(b) As used herein, “comparison window” includes reference to a contiguous and specified segment of a polynucleotide/polypeptide sequence, wherein the polynucleotide/polypeptide sequence may be compared to a reference sequence and wherein the portion of the polynucleotide/polypeptide sequence in the comparison window may comprise additions or deletions (i.e., gaps) compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. Generally, the comparison window is at least 20 contiguous nucleotides/amino acids residues in length, and optionally can be 30, 40, 50, 100, or longer. Those of skill in the art understand that to avoid a high similarity to a reference sequence due to inclusion of gaps in the polynucleotide/polypeptide sequence, a gap penalty is typically introduced and is subtracted from the number of matches.
Methods of alignment of sequences for comparison are well-known in the art. Optimal alignment of sequences for comparison may be conducted by the local homology algorithm of Smith and Waterman, 1981, Adv. Appl. Math. 2: 482; by the homology alignment algorithm of Needleman and Wunsch, 1970, J. Mol. Biol. 48: 443; by the search for similarity method of Pearson and Lipman, 1988, Proc. Natl. Acad. Sci. 85: 2444; by computerized implementations of these algorithms, including, but not limited to: CLUSTAL in the PC/Gene program by Intelligenetics, Mountain View, Calif.; GAP, BESTFIT, BLAST, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group (GCG), 575 Science Dr., Madison, Wis., USA; the CLUSTAL program is well described by Higgins and Sharp, 1988, Gene 73: 237-244; Higgins and Sharp, 1989, CABIOS 5: 151-153; Corpet et al., 1988, Nucleic Acids Research 16: 10881-90; Huang et al., 1992, Computer Applications in the Biosciences 8: 155-65; and Pearson et al., 1994, Methods in Molecular Biology 24: 307-331.
The BLAST family of programs which can be used for database similarity searches includes: BLASTN for nucleotide query sequences against nucleotide database sequences; BLASTX for nucleotide query sequences against protein database sequences; BLASTP for protein query sequences against protein database sequences; TBLASTN for protein query sequences against nucleotide database sequences; and TBLASTX for nucleotide query sequences against nucleotide database sequences. See, Current Protocols in Molecular Biology, Chapter 19, Ausubel et al., Eds., 1995, Greene Publishing and Wiley-Interscience, New York.
Software for performing BLAST analyses is publicly available, e.g., through the National Center for Biotechnology Information (world-wide web at ncbi.nlm.nih.gov). This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold. These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are then extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always >0) and N (penalty score for mismatching residues; always <0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) of 10, a cutoff of 100, M=5, N=−4, and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff & Henikoff, 1989, Proc. Natl. Acad. Sci. USA 89:10915).
In addition to calculating percent sequence identity, the BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g., Karlin & Altschul, 1993, Proc. Natl. Acad. Sci. USA 90:5873-5877). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance.
BLAST searches assume that proteins can be modeled as random sequences. However, many real proteins comprise regions of nonrandom sequences which may be homopolymeric tracts, short-period repeats, or regions enriched in one or more amino acids. Such low-complexity regions may be aligned between unrelated proteins even though other regions of the protein are entirely dissimilar. A number of low-complexity filter programs can be employed to reduce such low-complexity alignments. For example, the SEG (Wooten and Federhen, 1993, Comput. Chem., 17:149-163) and XNU (Claverie and States, 1993, Comput. Chem., 17:191-201) low-complexity filters can be employed alone or in combination.
Unless otherwise stated, nucleotide and protein identity/similarity values provided herein are calculated using GAP (GCG Version 10) under default values.
GAP (Global Alignment Program) can also be used to compare a polynucleotide or polypeptide of the present invention with a reference sequence. GAP uses the algorithm of Needleman and Wunsch (J. Mol. Biol. 48: 443-453, 1970) to find the alignment of two complete sequences that maximizes the number of matches and minimizes the number of gaps. GAP considers all possible alignments and gap positions and creates the alignment with the largest number of matched bases and the fewest gaps. It allows for the provision of a gap creation penalty and a gap extension penalty in units of matched bases. GAP must make a profit of gap creation penalty number of matches for each gap it inserts. If a gap extension penalty greater than zero is chosen, GAP must, in addition, make a profit for each gap inserted of the length of the gap times the gap extension penalty. Default gap creation penalty values and gap extension penalty values in Version 10 of the Wisconsin Genetics Software Package for protein sequences are 8 and 2, respectively. For nucleotide sequences the default gap creation penalty is 50 while the default gap extension penalty is 3. The gap creation and gap extension penalties can be expressed as an integer selected from the group of integers consisting of from 0 to 100. Thus, for example, the gap creation and gap extension penalties can each independently be: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 30, 40, 50, 60 or greater.
GAP presents one member of the family of best alignments. There may be many members of this family, but no other member has a better quality. GAP displays four figures of merit for alignments: Quality, Ratio, Identity, and Similarity. The Quality is the metric maximized in order to align the sequences. Ratio is the quality divided by the number of bases in the shorter segment. Percent Identity is the percent of the symbols that actually match. Percent Similarity is the percent of the symbols that are similar. Symbols that are across from gaps are ignored. A similarity is scored when the scoring matrix value for a pair of symbols is greater than or equal to 0.50, the similarity threshold. The scoring matrix used in Version 10 of the Wisconsin Genetics Software Package is BLOSUM62 (see Henikoff & Henikoff, 1989, Proc. Natl. Acad. Sci. USA 89:10915).
Multiple alignment of the sequences can be performed using the CLUSTAL method of alignment (Higgins and Sharp, 1989, CABIOS. 5:151-153) with the default parameters (GAP PENALTY=10, GAP LENGTH PENALTY=10). Default parameters for pairwise alignments using the CLUSTAL method are KTUPLE 1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5.
(c) As used herein, “sequence identity” or “identity” in the context of two nucleic acid or polypeptide sequences includes reference to the residues in the two sequences which are the same when aligned for maximum correspondence over a specified comparison window. When percentage of sequence identity is used in reference to proteins it is recognized that residue positions which are not identical often differ by conservative amino acid substitutions, where amino acid residues are substituted for other amino acid residues with similar chemical properties (e.g., charge or hydrophobicity) and therefore do not change the functional properties of the molecule. Where sequences differ in conservative substitutions, the percent sequence identity may be adjusted upwards to correct for the conservative nature of the substitution. Sequences which differ by such conservative substitutions are said to have “sequence similarity” or “similarity”. Means for making this adjustment are well-known to those of skill in the art. Typically this involves scoring a conservative substitution as a partial rather than a full mismatch, thereby increasing the percentage sequence identity. Thus, for example, where an identical amino acid is given a score of 1 and a non-conservative substitution is given a score of zero, a conservative substitution is given a score between zero and 1. The scoring of conservative substitutions is calculated, e.g., according to the algorithm of Meyers and Miller, 1988, Computer Applic. Biol. Sci., 4:11-17, e.g., as implemented in the program PC/GENE (Intelligenetics, Mountain View, Calif., USA).
Polynucleotide sequences having “substantial identity” are those sequences having at least about 50%, 60% sequence identity, generally 70% sequence identity, preferably at least 80%, more preferably at least 90%, and most preferably at least 95%, compared to a reference sequence using one of the alignment programs described above. Preferably sequence identity is determined using the default parameters determined by the program. Substantial identity of amino acid sequences generally means sequence identity of at least 50%, more preferably at least 70%, 80%, 90%, and most preferably at least 95%. Nucleotide sequences are generally substantially identical if the two molecules hybridize to each other under stringent conditions.
(d) As used herein, “percentage of sequence identity” means the value determined by comparing two optimally aligned sequences over a comparison window, wherein the portion of the polynucleotide sequence in the comparison window may comprise additions or deletions (i.e., gaps) as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. The percentage is calculated by determining the number of positions at which the identical nucleic acid base or amino acid residue occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison and multiplying the result by 100 to yield the percentage of sequence identity.
As used herein, the term “transgenic,” when used in reference to a plant (i.e., a “transgenic plant”) refers to a plant that contains at least one heterologous gene in one or more of its cells.
As used herein, “substantially complementary,” in reference to nucleic acids, refers to sequences of nucleotides (which may be on the same nucleic acid molecule or on different molecules) that are sufficiently complementary to be able to interact with each other in a predictable fashion, for example, producing a generally predictable secondary structure, such as a stem-loop motif. In some cases, two sequences of nucleotides that are substantially complementary may be at least about 75% complementary to each other, and in some cases, are at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, at least about 99.5%, or 100% complementary to each other. In some cases, two molecules that are sufficiently complementary may have a maximum of 40 mismatches (e.g., where one base of the nucleic acid sequence does not have a complementary partner on the other nucleic acid sequence, for example, due to additions, deletions, substitutions, bulges, etc.), and in other cases, the two molecules may have a maximum of 30 mismatches, 20 mismatches, 10 mismatches, or 7 mismatches. In still other cases, the two sufficiently complementary nucleic acid sequences may have a maximum of 0, 1, 2, 3, 4, 5, or 6 mismatches.
By “variants” is intended substantially similar sequences. For “variant” nucleotide sequences, conservative variants include those sequences that, because of the degeneracy of the genetic code, encode the amino acid sequence of the modulator of the invention. Variant nucleotide sequences include synthetically derived sequences, such as those generated, for example, using site-directed mutagenesis. Generally, variants of a particular nucleotide sequence of the invention will have at least about 40%, 50%, 60%, 65%, 70%, generally at least about 75%, 80%, 85%, preferably at least about 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, and more preferably at least about 98%, 99% or more sequence identity to that particular nucleotide sequence as determined by sequence alignment programs described elsewhere herein using default parameters. By “variant” protein is intended a protein derived from the native protein by deletion or addition of one or more amino acids to the N-terminal and/or C-terminal end of the native protein; deletion or addition of one or more amino acids at one or more sites in the native protein; or substitution of one or more amino acids at one or more sites in the native protein. Such variants may result from, for example, genetic polymorphism or human manipulation. Conservative amino acid substitutions will generally result in variants that retain biological function
As used herein, the term “yield” or “plant yield” refers to increased plant growth, and/or increased biomass. In one embodiment, increased yield results from increased growth rate and increased root size. In another embodiment, increased yield is derived from shoot growth. In still another embodiment, increased yield is derived from fruit growth.
5. DESCRIPTION OF THE FIGURES
FIG. 1. Schematic diagram of the nitrogen assimilation pathway.
FIG. 2. Schematic diagram of the effects of transgenic overexpression or underexpression of master regulators CCA1/GLK1/bZIP1 on nitrogen assimilation, usage and storage.
FIG. 3. Network analysis of genes regulated by organic N suggests CCA1 control N-assimilation in plants. In this Cytoscape-generated network, metabolic genes are drawn as triangles (transcription factors), hexagons (metabolic genes) whereas metabolites are shown as white circles. Arrows, diamonds or lines at the end of an edge indicate directionality of the interaction. To simplify, some of the genes connecting to GLK1 and CCA1 are grouped and summarized based on their associated functions (number in parenthesis indicate the number of genes in the group).
FIG. 4. Altered mRNA levels of target genes and binding of CCA1 protein to target gene promoter regions validate predicted regulation by CCA1. FIG. 4A: RT-qPCR was performed on CCA1-ox, glk1 knockout, and wild-type plants to determine mRNA levels for ASN1, GLN1.3, and GDH1. Three biological and two technical replicates were carried out for each sample. mRNA levels were normalized to clathrin (At4g24550). The mean+/−standard error of the mean is shown. FIG. 4B: ChIP assays to show binding of CCA1 to GLN1.3, GDH1 and bZIP1 gene promoter regions. Control: input DNA control (no IP), -Ab: IP without antibody, CCA1: IP with the CCA1 antibody.
FIG. 5. Exposure of seedlings to pulses of inorganic and organic N shifts the phase of the circadian clock. FIGS. 5A-C: Plot of the phase shift of CCA1::LUC expression in response to 4-h pulse of inorganic N (20 mM KNO3/20 mM NH4NO3), 10 mM Glu, or 10 mM Gln against the time at which the pulse was administered to wild-type seedlings. Pulses were administered at 3-hr intervals spanning one complete circadian cycle and data were collected over the next 6 cycles. Phase shifts are double-plotted to emphasize the circadian pattern of the response. Phase advances (the peak in expression occurring earlier) are plotted as positive values and delays are plotted as negative values. FIG. 5D shows the CCA1:LUC expression of control (untreated) seedlings. In all panels, the entraining photocycle (16:8) is indicated by the vertical white (light) and gray (dark) bars. The mean+/−standard error of the mean is shown.
FIG. 6. Proposed model of the interaction between the Arabidopsis circadian clock and N-assimilatory pathway. Arrows indicate influences that affect the function of the two processes. Black arrow: Clock function would affect N-assimilation. This influence is at least partly due to the direct regulatory role of CCA1 on N-assimilation. Grey arrow: N-assimilation would influence clock function through downstream metabolites such as Glu, Gln and possibly other N-metabolites.
FIG. 7. Signaling by inorganic vs. organic nitrogen can be distinguished by using MSX and Glu treatments. (a) Treatments include N±MSX±Glu. (b) A simplified diagram of the N-metabolic pathway from inorganic nitrate (NO3) to organic Glu, and the block by MSX. Below this pathway are the predicted effects of the given treatments on nitrogen metabolism. Arrows indicate progression through the pathway. Line breaks, represented with a short perpendicular line, indicate the step in the pathway blocked by MSX. (c and d). The expected transcript levels for genes induced by inorganic nitrogen (c) vs. genes regulated by Glu or a Glu-derived metabolite (d).
FIG. 8. Analysis of the expression of asparagine synthetase genes. Shown is a comparison of ASN1 (a) and ASN2 (b) mRNA levels in control seedlings (transferred to 1 mM NO3) along with MSX control (treated with NO3 and 1 mM MSX) compared to seedlings treated with a stepwise combination of Nms, MSX, and Glu/Gln. (a) mRNA levels of ASN1 are increased in Nms, are sensitive to MSX treatment, and can be recovered with exogenous application of Glu or Gln. (b) mRNA levels of ASN2 are increased in Nms. However, this expression is insensitive to MSX treatment and is slightly repressed with exogenous application of Glu or Gln. mRNA levels were normalized to EIF4A (At3g13920).
FIG. 9. RT-qPCR confirmation of the regulation for two transcription factors TAZ and bZIP1. Shown is confirmation of TAZ (FIG. 9A) and bZIP1 (FIG. 9B) mRNA levels in control seedlings along with the MSX control and compared to seedlings treated with a stepwise combination of Nms, Nms+MSX, and Nms+MSX+Glu (or Gln). In both cases, although increased expression in the presence of N is blocked in the presence of MSX, this suppression can be overcome by exogenous application of Glu or Gln. Plants transferred to control media do not show mRNA levels different from treatments without MSX. Primers used for RT-qPCR are as follows: TAZ forward, 5′-TCCTCGTCTCGGTCTT-3′ (SEQ ID NO:1); reverse, 5′-CAACCACCAGGGATTC-3′ (SEQ ID NO:2); bZIP forward, 5′-TCAGGTTCCGACATAGATG-3′ (SEQ ID NO:3); reverse, 5′-CCACGGTGTACGTCTACA-3′ (SEQ ID NO:4).
FIG. 10. Analysis of the expression of bZIP1 in the CCA1-ox. To test some of the predictions of our network CCA1-ox and Col-0 plants were collected 3 h after dawn; three biological replicates were taken at each time point. RNA was extracted from whole seedlings (as described in Materials and Methods), and RT-qPCR was performed to measure mRNA levels for bZIP1 (At5g49450). Two technical replicates were carried out for each sample. mRNA levels were normalized to clathrin (At4g24550).
FIG. 11. Circadian regulation of the response of clock gene (CCA1) expression to N-assimilation inhibitors and inorganic and organic N. Mean±SEM luciferase activity of CCA1::LUC in response to exogenous inorganic N, Glu, or Gln is presented. Seedlings were entrained for 8 days in a 16-h white light/8-h dark photoperiod on MS medium containing 1 mM KNO3 before being transferred to continuous light and exposed for 4-h pulses of inorganic N (20 mM KNO3/20 mM NH4NO3), 10 mM Glu, or 10 mM Gln presented at 3-h intervals over one circadian cycle before return to MS medium containing 1 mM KNO3 in continuous light for luciferase measurements for 6 days. Luciferase activity values were normalized by the mean expression value for the treatment. The entraining photocycle is indicated by the vertical white (light) and gray (dark) bars.
FIG. 12. Schematic diagram of how CCA1/GLK1/bZIP1 transcription factors coordinate the nitrogen regulation of genes in the nitrogen assimilation pathway.
6. DETAILED DESCRIPTION
Master control genes (CCA1, GLK1, and bZIP11) that control N-assimilation in response to Glu sensing have been identified in the present invention. As these genes are transcription factor hubs, they coordinate the N-regulation of the N-assimilatory gene network, with genome-wide responses associated with growth and development in plants. Thus, effecting genome-wide changes in N-assimilation, plant growth and development, by the transgenic manipulation of these master control genes in plants effects nitrogen use efficiency in vegetative tissues (leaves & roots) and also in seed. Changes in levels of N-assimilated into Gln effect changes in growth of vegetative tissues, while changes in levels of Asn affect seed development.
Thus, the present invention relates to the transgenic manipulation of these N-responsive master regulatory genes (CCA1, GLK1, and bZIP11) that control N-assimilation, and other related processes in response to N treatments, so as to increase the overall N-assimilation capacity, whether for increased N usage or N storage. The overexpression of these master control genes (e.g., uncoupled from Glu repression) effectively releases N-assimilation from the feedback repression loop by Glu-leading to increased N-assimilation and usage. As these regulatory genes serve to respond to Glu levels by reciprocally regulating the amount of N-assimilated into Gln versus the amount of Gln metabolized to Asn (for N-storage and transport), the manipulation of these genes in transgenic plants can be used to optimize N-assimilation into Gln versus Asn (FIG. 6). Increased N-assimilation is advantageous in all crops. Additionally, in seed crops, the increased synthesis of Asn increases N-transported and stored in seed.
Thus, in one embodiment, the present invention is directed to a method for improving nitrogen assimilation and usage in a plant in which more nitrogen is available for biosynthesis, said method comprising overexpressing GLK1 in the plant. In another embodiment, the present invention is directed to a method for improving nitrogen assimilation and usage in a plant in which more nitrogen is available for biosynthesis, said method comprising overexpressing CCA1 in the plant. In yet another embodiment, the present invention is directed to a method for improving nitrogen assimilation and usage in a plant in which more nitrogen is available for biosynthesis, said method comprising underexpressing bZIP1 in the plant.
In another embodiment, the method for improving nitrogen assimilation and usage in a plant in which more nitrogen is available for biosynthesis comprises overexpressing CCA1 and GLK1 in the plant. In another embodiment, the method for improving nitrogen assimilation and usage in a plant in which more nitrogen is available for biosynthesis comprises overexpressing CCA1 and underexpressing bZIP1 in the plant, or overexpressing GLK1 and underexpressing bZIP1 in the plant. In yet another embodiment, the method for improving nitrogen assimilation and usage in a plant in which more nitrogen is available for biosynthesis comprises overexpressing CCA1, overexpressing GLK1 and underexpressing bZIP1 in the plant.
The present invention is also directed to methods for altering nitrogen assimilation and storage, e.g., increasing nitrogen storage, in a plant. In one embodiment, the method comprises overexpressing bZIP1 in the plant. In another embodiment, the method comprises underexpressing CCA1 in the plant and/or underexpressing GLK1 in the plant. In another embodiment, the method overexpressing bZIP1 and underexpressing CCA1 and/or GLK1 in the plant. In yet another embodiment, the method comprises overexpressing bZIP1, and underexpressing CCA1 and underexpressing GLK1 in the plant.
In certain embodiments, the plant is species of woody, ornamental, decorative, crop, cereal, fruit, or vegetable. In other embodiments, the plant is a species of one of the following genuses: Acorus, Aegilops, Allium, Amborella, Antirrhinum, Apium, Arabidopsis, Arachis, Beta, Betula, Brassica, Capsicum, Ceratopteris, Citrus, Cryptomeria, Cycas, Descurainia, Eschscholzia, Eucalyptus, Glycine, Gossypium, Hedyotis, Helianthus, Hordeum, Ipomoea, Lactuca, Linum, Liriodendron, Lotus, Lupinus, Lycopersicon, Medicago, Mesembryanthemum, Nicotiana, Nuphar, Pennisetum, Persea, Phaseolus, Physcomitrella, Picea, Pinus, Poncirus, Populus, Prunus, Robinia, Rosa, Saccharum, Schedonorus, Secale, Sesamum, Solanum, Sorghum, Stevia, Thellungiella, Theobroma, Triphysaria, Triticum, Vitis, Zea, or Zinnia.
The overexpression of a particular gene can be accomplished by any method known in the art, for example, by transforming a plant cell with a nucleic acid vector comprising the coding sequences of the desired gene operably linked to a promoter active in a plant cell such that the desired gene is expressed at levels higher than normal, i.e., levels found in a control/nontransgenic plant. Such promoters can be constitutively active in all or some plant tissues or can be inducible.
The underexpression of a desired gene can be accomplished by any method known in the art, such as knocking out the gene or mutating the gene transgenically such that lower than normal levels of the gene product is produced in the transgenic cells or plant. For example, such mutations include frame-shift mutations or mutations resulting in a stop codon in the wild-type coding sequence, thus preventing expression of the gene product. Another exemplary mutation would be the removal of the transcribed sequences from the plant genome, for example, by homologous recombination. Another method for underexpressing a gene is transgenically introducing an insertion or deletion into the transcribed sequence or an insertion or deletion upstream or downstream of the transcribed sequence such that expression of the gene product is decreased as compared to wild-type or appropriate control. Additionally, microRNA (native or artificial) can be used to target a particular encoding mRNA for degradation, thus reducing the level of the expressed gene product in the transgenic plant cell.
The present invention is also directed to a transgenic plant produced by any of the foregoing methods.
The present invention is also directed to compositions for modulating gene expression in plants. The compositions comprise constructs for the expression of CCA1, GLK1 or bZIP1. In certain embodiments, a construct of the invention comprises a promoter, such as a tissue specific promoter, which is expressed in a plant cell, such as a leaf cell, and promotes the expression of CCA1, GLK1 or bZIP1.
Any of a variety of promoters can be utilized in the constructs of the invention depending on the desired outcome. Tissue-specific or tissue-preferred promoters, inducible promoters, developmental promoters, constitutive promoters and/or chimeric promoters can be used to direct expression of the gene product in specific cells or organs the plant, when fused to the appropriate cell or organ specific promoter.
Chimeric constructs expressing CCA1, GLK1 or bZIP1 in transgenic plants (using constitutive or inducible promoters) can be used in the compositions and methods provided herein to enhance nitrogen assimilation and usage or increase nitrogen storage.
The present invention is also directed to a transgenic plant-derived commercial product. In one embodiment, the transgenic plant is a tree, and said commercial product is pulp, paper, a paper product, or lumber. In another embodiment, the transgenic plant is tobacco, and said commercial product is a cigarette, cigar, or chewing tobacco. In yet another embodiment, the transgenic plant is a crop, and said commercial product is a fruit or vegetable. In yet another embodiment, the transgenic plant is a grain, and said commercial product is bread, flour, cereal, oat meal, or rice. In another embodiment, the product is a biofuel or a plant oil.
6.1 Master Regulators
CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) gene encodes a MYB-related transcription factor involved in the phytochrome induction of a light-harvesting chlorophyll a/b-protein gene. The nucleotide and amino acid sequences of CCA1 from Arabidopsis are known, see Accession No. At2g46830. Further, orthologous CCA1 genes from other organisms are also known. For example, the CCA1 gene sequences from poplar can be found under Accession Nos. Poptr1#552368 or Poptr1#731468. The use of CCA1 in the present invention refers not only to the Arabidopsis gene but also the orthologous CCA1 gene from other species. Thus, in one embodiment, plant species-specific CCA1 genes can be used in plants of the same species, e.g., tobacco CCA1 can be overexpressed in tobacco. Additionally, such orthologous sequences can be identified and isolated using methods known in the art, such as hybridization methods and then testing the isolated sequences for CCA1 activity, as demonstrated infra. Other methods, such as alignment methods described supra can also be used to identify and isolate orthologous CCA1 sequences.
Golden 2-like genes (GLK) are members of the GARP superfamily of transcription factors. GLK genes are known to be involved in the regulation of chloroplast development in diverse plant species (Fritter et al., 2002, The Plant Journal 31:713-727). The nucleotide and amino acid sequences of GLK1 from Arabidopsis are known, see Accession No. At2g20570. Further, orthologous GLK1 genes from other organisms are also known. For example, the GLK1 gene sequences from poplar and rice can be found under Accession Nos. Poptr1#654401 and Os06g24070, respectively. The use of GLK1 in the present invention refers not only to the Arabidopsis gene but also the orthologous GLK1 gene from other species. Thus, in one embodiment, plant species-specific GLK1 genes can be used in plants of the same species, e.g., tobacco GLK1 can be overexpressed in tobacco. Additionally, such orthologous sequences can be identified and isolated using methods known in the art, such as hybridization methods and then testing the isolated sequences for GLK1 activity, such as DNA binding activity. Other methods, such as alignment methods described supra can also be used to identify and isolate orthologous GLK1 sequences.
bZIP1 is a transcription factor that belongs to the largest bZIP group in Arabadopsis, Group S (Jakoby et al., 2002, Trends Plant Sci 7:106-111). It is thought that Group S bZIP genes are involved in balancing carbohydrate demand and supply (Rook et al., 1998, Plant J 15:253-263). The nucleotide and amino acid sequences of bZIP1 from Arabidopsis are known, see Accession No. At5g49450. The use of bZIP1 in the present invention refers not only to the Arabidopsis gene but also the orthologous bZIP1 gene from other species. Thus, in one embodiment, plant species-specific bZIP1 genes can be used in plants of the same species, e.g., tobacco bZIP1 can be overexpressed in tobacco. Additionally, such orthologous sequences can be identified and isolated using methods known in the art, such as hybridization methods and then testing the isolated sequences for bZIP1 activity, such as DNA binding activity. Other methods, such as alignment methods described supra can also be used to identify and isolate orthologous bZIP1 sequences.
6.2 Modulation of Gene Expression
The methods of the invention involve modulation of the expression of one, two, three or more target nucleotide sequences in a plant, optionally in specific tissues such as vegetative tissues or leaves or seeds. That is, the expression of a target nucleotide sequence of interest may be increased or decreased. In specific embodiments, the target nucleotide sequences are CCA1, GLK1 or bZIP1, which can be increased or decreased.
The target nucleotide sequences may be endogenous or exogenous in origin. By “modulate expression of a target gene” is intended that the expression of the target gene is increased or decreased relative to the expression level in a plant that has not been altered by the methods described herein.
By “increased or over expression” is intended that expression of the target nucleotide sequence is increased over expression observed in conventional transgenic lines for heterologous genes and over endogenous levels of expression for homologous genes. Heterologous or exogenous genes comprise genes that do not occur in the plant of interest in its native state. Homologous or endogenous genes are those that are natively present in the plant genome. Generally, expression of the target sequence is substantially increased. That is expression is increased at least about 25%-50%, preferably about 50%-100%, more preferably about 100%, 200% and greater.
By “decreased expression” or “underexpression” it is intended that expression of the target nucleotide sequence is decreased below expression observed in conventional transgenic lines for heterologous genes and below endogenous levels of expression for homologous genes. Generally, expression of the target nucleotide sequence of interest is substantially decreased. That is expression is decreased at least about 25%-50%, preferably about 50%-100%, more preferably about 100%, 200% and greater.
Expression levels may be assessed by determining the level of a gene product by any method known in the art including, but not limited to determining the levels of the RNA and protein encoded by a particular target gene. For genes that encode proteins, expression levels may determined, for example, by quantifying the amount of the protein present in plant cells, or in a plant or any portion thereof. Alternatively, it desired target gene encodes a protein that has a known measurable activity, then activity levels may be measured to assess expression levels.
6.3 Transformation/Transfection
Any method or delivery system may be used for the delivery and/or transfection of the nucleic acid vectors encoding any of the master regulators of the present invention in the cell. The vectors may be delivered to the plant cell either alone, or in combination with other agents.
Transfection may be accomplished by a wide variety of means, as is known to those of ordinary skill in the art. Such methods include, but are not limited to, Agrobacterium-mediated transformation (e.g., Komari et al., 1998, Curr. Opin. Plant Biol., 1:161), particle bombardment mediated transformation (e.g., Finer et al., 1999, Curr. Top. Microbiol. Immunol., 240:59), protoplast electroporation (e.g., Bates, 1999, Methods Mol. Biol., 111:359), viral infection (e.g., Porta and Lomonossoff, 1996, Mol. Biotechnol. 5:209), microinjection, and liposome injection. Other exemplary delivery systems that can be used to facilitate uptake by a cell of the nucleic acid include calcium phosphate and other chemical mediators of intracellular transport, microinjection compositions, and homologous recombination compositions (e.g., for integrating a gene into a preselected location within the chromosome of the cell). Alternative methods may involve, for example, the use of liposomes, electroporation, or chemicals that increase free (or “naked”) DNA uptake, transformation using viruses or pollen and the use of microprojection. Standard molecular biology techniques are common in the art (e.g., Sambrook et al., 1989, Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, New York). For example, in one embodiment of the present invention, Arabidopsis or another plant species is transformed with a gene encoding CCA1, GLK1 or bZIP1 using Agrobacterium.
One of skill in the art will be able to select an appropriate vector for introducing the encoding nucleic acid sequence in a relatively intact state. Thus, any vector which will produce a plant carrying the introduced encoding nucleic acid should be sufficient. The selection of the vector, or whether to use a vector, is typically guided by the method of transformation selected.
The transformation of plants in accordance with the invention may be carried out in essentially any of the various ways known to those skilled in the art of plant molecular biology. (See, for example, Methods of Enzymology, Vol. 153, 1987, Wu and Grossman, Eds., Academic Press, incorporated herein by reference).
Plant cells and plants can comprise two or more nucleotide sequence constructs. Any means for producing a plant comprising the nucleotide sequence constructs described herein are encompassed by the present invention. For example, a nucleotide sequence encoding the modulator can be used to transform a plant at the same time as the nucleotide sequence encoding the precursor RNA. The nucleotide sequence encoding the precursor mRNA can be introduced into a plant that has already been transformed with the modulator nucleotide sequence. Alternatively, transformed plants, one expressing the modulator and one expressing the RNA precursor, can be crossed to bring the genes together in the same plant. Likewise, viral vectors may be used to express gene products by various methods generally known in the art. Suitable plant viral vectors for expressing genes should be self-replicating, capable of systemic infection in a host, and stable. Additionally, the viruses should be capable of containing the nucleic acid sequences that are foreign to the native virus forming the vector. Transient expression systems may also be used.
Agrobacterium transformation is widely used by those skilled in the art to transform dicotyledonous species. Recently, there has been substantial progress towards the routine production of stable, fertile transgenic plants in almost all economically relevant monocot plants (Toriyarna et al., 1988, Bio/Technology 6:1072-1074; Zhang et al., 1988, Plant Cell Rep. 7:379-384; Zhang et al., 1988, Theor. Appl. Genet. 76:835-840; Shimamoto et al., 1989, Nature 338:274-276; Datta et al., 1990, Bio/Technology 8: 736-740; Christou et al., 1991, Bio/Technology 9:957-962; Peng et al., 1991, International Rice Research Institute, Manila, Philippines, pp. 563-574; Cao et al., 1992, Plant Cell Rep. 11:585-591; Li et al., 1993, Plant Cell Rep. 12:250-255; Rathore et al., 1993, Plant Mol. Biol. 21:871-884; Fromm et al., 1990, Bio/Technology 8:833-839; Tomes et al., 1995, “Direct DNA Transfer into Intact Plant Cells via Microprojectile Bombardment,” in Plant Cell, Tissue, and Organ Culture: Fundamental Methods, ed. Gamborg and Phillips (Springer-Verlag, Berlin); D'Halluin et al., 1992, Plant Cell 4:1495-1505; Walters et al., 1992, Plant Mol. Biol. 18:189-200; Koziel et al., 1993, Biotechnology 11: 194-200; Vasil, I. K., 1994, Plant Mol. Biol. 25:925-937; Weeks et al., 1993, Plant Physiol. 102:1077-1084; Somers et al., 1992, Bio/Technology 10: 1589-1594; WO 92/14828). In particular, Agrobacterium mediated transformation is now emerging also as an highly efficient transformation method in monocots (Hiei et al., 1994, The Plant Journal 6:271-282). See also, Shimamoto, K., 1994, Current Opinion in Biotechnology 5:158-162; Vasil et al., 1992, Bio/Technology 10:667-674; Vain et al., 1995, Biotechnology Advances 13(4):653-671; Vasil et al., 1996, Nature Biotechnology 14:702).
The particular choice of a transformation technology will be determined by its efficiency to transform certain plant species as well as the experience and preference of the person practicing the invention with a particular methodology of choice. It will be apparent to the skilled person that the particular choice of a transformation system to introduce nucleic acid into plant cells is not essential to or a limitation of the invention, nor is the choice of technique for plant regeneration.
6.3.1 Agrobacterium
A CCA1, GLK1 or bZIP1-encoding nucleic acid sequences or a nucleic acid designed to disrupt expression of CCA1, GLK1 or bZIP1 utilized in the present invention can be introduced into plant cells using Ti plasmids of Agrobacterium tumefaciens (A. tumefaciens), root-inducing (Ri) plasmids of Agrobacterium rhizogenes (A. rhizogenes), and plant virus vectors. For reviews of such techniques see, for example, Weissbach & Weissbach, 1988, Methods for Plant Molecular Biology, Academic Press, NY, Section VIII, pp. 421-463; and Grierson & Corey, 1988, Plant Molecular Biology, 2d Ed., Blackie, London, Ch. 7-9, and Horsch et al., 1985, Science, 227:1229.
In using an A. tumefaciens culture as a transformation vehicle, it is most advantageous to use a non-oncogenic strain of Agrobacterium as the vector carrier so that normal non-oncogenic differentiation of the transformed tissues is possible. It is also preferred that the Agrobacterium harbor a binary Ti plasmid system. Such a binary system comprises 1) a first Ti plasmid having a virulence region essential for the introduction of transfer DNA (T-DNA) into plants, and 2) a chimeric plasmid. The chimeric plasmid contains at least one border region of the T-DNA region of a wild-type Ti plasmid flanking the nucleic acid to be transferred. Binary Ti plasmid systems have been shown effective in the transformation of plant cells (De Framond, Biotechnology, 1983, 1:262; Hoekema et al., 1983, Nature, 303:179). Such a binary system is preferred because it does not require integration into the Ti plasmid of A. tumefaciens, which is an older methodology.
In some embodiments, a disarmed Ti-plasmid vector carried by Agrobacterium exploits its natural gene transferability (EP-A-270355, EP-A-01 16718, Townsend et al., 1984, NAR, 12:8711, U.S. Pat. No. 5,563,055).
Methods involving the use of Agrobacterium in transformation according to the present invention include, but are not limited to: 1) co-cultivation of Agrobacterium with cultured isolated protoplasts; 2) transformation of plant cells or tissues with Agrobacterium; or 3) transformation of seeds, apices or meristems with Agrobacterium.
In addition, gene transfer can be accomplished by in planta transformation by Agrobacterium, as described by Bechtold et al., (C. R. Acad. Sci. Paris, 1993, 316:1194). This approach is based on the vacuum infiltration of a suspension of Agrobacterium cells.
In certain embodiments, a CCA1, GLK1, bZIP1-encoding nucleic acid or mutant thereof is introduced into plant cells by infecting such plant cells, an explant, a meristem or a seed, with transformed A. tumefaciens as described above. Under appropriate conditions known in the art, the transformed plant cells are grown to form shoots, roots, and develop further into plants.
Other methods described herein, such as microprojectile bombardment, electroporation and direct DNA uptake can be used where Agrobacterium is inefficient or ineffective. Alternatively, a combination of different techniques may be employed to enhance the efficiency of the transformation process, e.g., bombardment with Agrobacterium-coated microparticles (EP-A-486234) or microprojectile bombardment to induce wounding followed by co-cultivation with Agrobacterium (EP-A-486233).
6.3.2 CaMV
In some embodiments, cauliflower mosaic virus (CaMV) is used as a vector for introducing a desired nucleic acid into plant cells (U.S. Pat. No. 4,407,956). CaMV viral DNA genome can be inserted into a parent bacterial plasmid creating a recombinant DNA molecule which can be propagated in bacteria. After cloning, the recombinant plasmid again can be cloned and further modified by introduction of the desired nucleic acid sequence. The modified viral portion of the recombinant plasmid can then be excised from the parent bacterial plasmid, and used to inoculate the plant cells or plants.
6.3.3 Mechanical and Chemical Means
In some embodiments, a CCA1, GLK1 or bZIP1-encoding nucleic acid or a nucleic acid designed to disrupt expression of CCA1, GLK1 or bZIP1 is introduced into a plant cell using mechanical or chemical means. Exemplary mechanical and chemical means are provided below.
As used herein, the term “contacting” refers to any means of introducing a CCA1, GLK1 or bZIP1-encoding nucleic acid or a nucleic acid designed to disrupt expression of CCA1, GLK1 or bZIP1 into a plant cell, including chemical and physical means as described above. Preferably, contacting refers to introducing the nucleic acid or vector containing the nucleic acid into plant cells (including an explant, a meristem or a seed), via A. tumefaciens transformed with the, e.g., GLK1-encoding nucleic acid as described above.
6.3.3.1 Microinjection
In one embodiment, the CCA1, GLK1 or bZIP1-encoding nucleic acid or the nucleic acid designed to disrupt expression of CCA1, GLK1 or bZIP1 can be mechanically transferred into the plant cell by microinjection using a micropipette. See, e.g., WO 92/09696, WO 94/00583, EP 331083, EP 175966, Green et al., 1987, Plant Tissue and Cell Culture, Academic Press, Crossway et al., 1986, Biotechniques 4:320-334.
6.3.3.2 PEG
In other embodiment, the nucleic acid can also be transferred into the plant cell by using polyethylene glycol (PEG) which forms a precipitation complex with genetic material that is taken up by the cell.
6.3.3.3 Electroporation
Electroporation can be used, in another set of embodiments, to deliver a nucleic acid to the cell, e.g., precursor miRNA, or a nucleotide sequence able to be transcribed to produce CCA1, GLK1 or bZIP1 protein (see, e.g., Fromm et al., 1985, PNA5, 82:5824). “Electroporation,” as used herein, is the application of electricity to a cell, such as a plant protoplast, in such a way as to cause delivery of a nucleic acid into the cell without killing the cell. Typically, electroporation includes the application of one or more electrical voltage “pulses” having relatively short durations (usually less than 1 second, and often on the scale of milliseconds or microseconds) to a media containing the cells. The electrical pulses typically facilitate the non-lethal transport of extracellular nucleic acids into the cells. The exact electroporation protocols (such as the number of pulses, duration of pulses, pulse waveforms, etc.), will depend on factors such as the cell type, the cell media, the number of cells, the substance(s) to be delivered, etc., and can be determined by those of ordinary skill in the art. Electroporation is discussed in greater detail in, e.g., EP 290395, WO 8706614, Riggs et al., 1986, Proc. Natl. Acad. Sci. USA 83:5602-5606; D'Halluin et al., 1992, Plant Cell 4:1495-1505). Other forms of direct DNA uptake can also be used in the methods provided herein, such as those discussed in, e.g., DE 4005152, WO 9012096, U.S. Pat. No. 4,684,611, Paszkowski et al., 1984, EMBO J. 3:2717-2722.
6.3.3.4 Ballistic and Particle Bombardment
Another method for introducing a CCA1, GLK1 or bZIP1-encoding nucleic acid or a nucleic acid designed to disrupt expression of CCA1, GLK1 or bZIP1 into a plant cell is high velocity ballistic penetration by small particles with the nucleic acid to be introduced contained either within the matrix of such particles, or on the surface thereof (Klein et al., 1987, Nature 327:70). Genetic material can be introduced into a cell using particle gun (“gene gun”) technology, also called microprojectile or microparticle bombardment. In this method, small, high-density particles (microprojectiles) are accelerated to high velocity in conjunction with a larger, powder-fired macroprojectile in a particle gun apparatus. The microprojectiles have sufficient momentum to penetrate cell walls and membranes, and can carry RNA or other nucleic acids into the interiors of bombarded cells. It has been demonstrated that such microprojectiles can enter cells without causing death of the cells, and that they can effectively deliver foreign genetic material into intact tissue. Bombardment transformation methods are also described in Sanford et al. (Techniques 3:3-16, 1991) and Klein et al. (Bio/Techniques 10:286, 1992). Although, typically only a single introduction of a new nucleic acid sequence(s) is required, this method particularly provides for multiple introductions.
Particle or microprojectile bombardment are discussed in greater detail in, e.g., the following references: U.S. Pat. No. 5,100,792, EP-A-444882, EP-A-434616; Sanford et al., U.S. Pat. No. 4,945,050; Tomes et al., 1995, “Direct DNA Transfer into Intact Plant Cells via Microprojectile Bombardment,” in Plant Cell, Tissue, and Organ Culture: Fundamental Methods, ed. Gamborg and Phillips (Springer-Verlag, Berlin); and McCabe et al., 1988, Biotechnology 6:923-926.
6.3.3.5 Colloidal Dispersion
In other embodiments, a colloidal dispersion system may be used to facilitate delivery of a nucleic acid into the cell, for example, GLK1, or a nucleotide sequence able to disrupt expression of GLK1. As used herein, a “colloidal dispersion system” refers to a natural or synthetic molecule, other than those derived from bacteriological or viral sources, capable of delivering to and releasing the nucleic acid to the cell. Colloidal dispersion systems include, but are not limited to, macromolecular complexes, beads, and lipid-based systems including oil-in-water emulsions, micelles, mixed micelles, and liposomes. One example of a colloidal dispersion system is a liposome. Liposomes are artificial membrane vessels. It has been shown that large unilamellar vessels (“LUV”), which-range in size from 0.2 to 4.0 microns, can encapsulate large macromolecules within the aqueous interior and these macromolecules can be delivered to cells in a biologically active form (e.g., Fraley et al., 1981, Trends Biochem. Sci., 6:77).
6.3.3.6 Lipids
Lipid formulations for the transfection and/or intracellular delivery of nucleic acids are commercially available, for instance, from QIAGEN, for example as EFFECTENE® (a non-liposomal lipid with a special DNA condensing enhancer) and SUPER-FECT® (a novel acting dendrimeric technology) as well as Gibco BRL, for example, as LIPOFECTIN® and LIPOFECTACE®, which are formed of cationic lipids such as N-[1-(2,3-dioleyloxy)-propyl]-N,N,N-trimethylammonium chloride (“DOTMA”) and dimethyl dioctadecylammonium bromide (“DDAB”). Liposomes are well known in the art and have been widely described in the literature, for example, in Gregoriadis, G., 1985, Trends in Biotechnology 3:235-241; Freeman et al., 1984, Plant Cell Physiol. 29:1353).
6.3.3.7 Other Methods
In addition to the above, other physical methods for the transformation of plant cells are reviewed in the following and can be used in the methods provided herein. Oard, 1991, Biotech. Adv. 9:1-11. See generally, Weissinger et al., 1988, sAnn. Rev. Genet. 22:421-477; Sanford et al., 1987, Particulate Science and Technology 5:27-37; Christou et al., 1988, Plant Physiol. 87:671-674; McCabe et al., 1988, Bio/Technology 6:923-926; Finer and McMullen, 1991, In vitro Cell Dev. Biol. 27P:175-182; Singh et al., 1998, Theor. Appl. Genet. 96:319-324; Datta et al., 1990, Biotechnology 8:736-740; Klein et al., 1988, Proc. Natl. Acad. Sci. USA 85:4305-4309; Klein et al., 1988, Biotechnology 6:559-563; Tomes, U.S. Pat. No. 5,240,855; Buising et al., U.S. Pat. Nos. 5,322,783 and 5,324,646; Klein et al., 1988, Plant Physiol. 91:440-444; Fromm et al., 1990, Biotechnology 8:833-839; Hooykaas-Van Slogteren et al., 1984, Nature (London) 311:763-764; Bytebier et al., 1987, Proc. Natl. Acad. Sci. USA 84:5345-5349; De Wet et al., 1985, The Experimental Manipulation of Ovule Tissues, ed. Chapman et al. (Longman, N.Y.), pp. 197-209; Kaeppler et al., 1990, Plant Cell Reports 9:415-418 and Kaeppler et al., 1992, Theor. Appl. Genet. 84:560-566; Li et al., 1993, Plant Cell Reports 12:250-255 and Christou and Ford, 1995, Annals of Botany 75:407-413; Osjoda et al., 1996, Nature Biotechnology 14:745-750; all of which are herein incorporated by reference.
6.4 Nucleic Acid Constructs
The CCA1, GLK1, bZIP1 sequences of the invention may be provided in nucleotide sequence constructs or expression cassettes for expression in the plant of interest. The cassette will include 5′ and 3′ regulatory sequences operably linked to an encoding nucleotide sequence of the invention.
The expression cassette may additionally contain at least one additional gene to be co-transformed into the organism. Alternatively, the additional gene(s) can be provided on multiple expression cassettes.
In certain embodiments, an expression cassette can be used with a plurality of restriction sites for insertion of the sequences of the invention to be under the transcriptional regulation of the regulatory regions. The expression cassette can additionally contain selectable marker genes (see below).
The expression cassette will generally include in the 5′-3′ direction of transcription, a transcriptional and translational initiation region, a DNA sequence of the invention, e.g., GLK1 or a sequence designed to disrupt expression of GLK1, and a transcriptional and translational termination region functional in plants. The transcriptional initiation region, the promoter, may be native or analogous or foreign or heterologous to the plant host. Additionally, the promoter may be the natural sequence or alternatively a synthetic sequence. By “foreign” is intended that the transcriptional initiation region is not found in the native plant into which the transcriptional initiation region is introduced. As used herein, a chimeric gene comprises a coding sequence operably linked to a transcription initiation region that is heterologous to the coding sequence.
The termination region may be native with the transcriptional initiation region, may be native with the operably linked DNA sequence of interest, or may be derived from another source. Convenient termination regions are available from the Ti-plasmid of A. tumefaciens, such as the octopine synthase and nopaline synthase termination regions. See also Guerineau et al., 1991, Mol. Gen. Genet. 262:141-144; Proudfoot, 1991, Cell 64:671-674; Sanfacon et al., 1991, Genes Dev. 5:141-149; Mogen et al., 1990, Plant Cell 2:1261-1272; Munroe et al., 1990, Gene 91:151-158; Ballas et al., 1989, Nucleic Acids Res. 17:7891-7903; and Joshi et al., 1987, Nucleic Acid Res. 15:9627-9639.
In some embodiments, a nucleic acid (e.g., encoding GLK1 or bZIP1) can be delivered to the cell in a vector. As used herein, a “vector” is any vehicle capable of facilitating the transfer of the nucleic acid to the cell such that the nucleic acid can be processed and/or expressed in the cell. The vector may transport the nucleic acid to the cells with reduced degradation, relative to the extent of degradation that would result in the absence of the vector. The vector optionally includes gene expression sequences or other components (such as promoters and other regulatory elements) able to enhance expression of the nucleic acid within the cell. The invention also encompasses the cells transfected with these vectors, including those cells previously described. In certain embodiments, the cells are transfected or transformed with a vector that specifically (or preferably) overexpresses CCA1 and/or GLK1 in the vegetative tissues of the plant, but not in the majority of other cell types of the plant.
To commence a transformation process in certain embodiments, it is first necessary to construct a suitable vector and properly introduce it into the plant cell. Vector(s) employed in the present invention for transformation of a plant cell include an encoding nucleic acid sequence operably associated with a promoter, such as a leaf-specific promoter. Details of the construction of vectors utilized herein are known to those skilled in the art of plant genetic engineering.
In general, vectors useful in the invention include, but are not limited to, plasmids, phagemids, viruses, other vehicles derived from viral or bacterial sources that have been manipulated by the insertion or incorporation of the nucleotide sequences (or precursor nucleotide sequences) of the invention. Viral vectors useful in certain embodiments include, but are not limited to, nucleic acid sequences from the following viruses: retroviruses; adenovirus, or other adeno-associated viruses; mosaic viruses such as tobamoviruses; potyviruses, nepoviruses, and RNA viruses such as retroviruses. One can readily employ other vectors not named but known to the art. Some viral vectors can be based on non-cytopathic eukaryotic viruses in which non-essential genes have been replaced with the nucleotide sequence of interest. Non-cytopathic viruses include retroviruses, the life cycle of which involves reverse transcription of genomic viral RNA into DNA with subsequent proviral integration into host cellular DNA.
Genetically altered retroviral expression vectors can have general utility for the high-efficiency transduction of nucleic acids. Standard protocols for producing replication-deficient retroviruses (including the steps of incorporation of exogenous genetic material into a plasmid, transfection of a packaging cell lined with plasmid, production of recombinant retroviruses by the packaging cell line, collection of viral particles from tissue culture media, and infection of the cells with viral particles) are well known to those of ordinary skill in the art. Examples of standard protocols can be found in Kriegler, M., 1990, Gene Transfer and Expression, A Laboratory Manual, W.H. Freeman Co., New York, or Murry, E. J. Ed., 1991, Methods in Molecular Biology, Vol. 7, Humana Press, Inc., Cliffton, N.J.
Another-example of a virus for certain applications is the adeno-associated virus, which is a double-stranded DNA virus. The adeno-associated virus can be engineered to be replication-deficient and is capable of infecting a wide range of-cell types and species. The adeno-associated virus further has advantages, such as heat and lipid solvent stability; high transduction frequencies in cells of diverse lineages; and/or lack of superinfection inhibition, which may allow multiple series of transductions.
Another vector suitable for use with the method provided herein is a plasmid vector. Plasmid vectors, have been extensively described in the art and are well-known to those of skill in the art. See, e.g., Sambrook et al., 1989, Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor Laboratory Press. These plasmids may have a promoter compatible with the host cell, and the plasmids can express a peptide from a gene operatively encoded within the plasmid. Some commonly used plasmids include pBR322, pUC18, pUC19, pRC/CMV, SV40, and pBlueScript. Other plasmids are well-known to those of ordinary skill in the art. Additionally, plasmids may be custom-designed, for example, using restriction enzymes and ligation reactions, to remove and add specific fragments of DNA or other nucleic acids, as necessary. The present invention also includes vectors for producing nucleic acids or precursor nucleic acids containing a desired nucleotide sequence (which can, for instance, then be cleaved or otherwise processed within the cell to produce a precursor miRNA). These vectors may include a sequence encoding a nucleic acid and an in vivo expression element, as further described below. In some cases, the in vivo expression element includes at least one promoter.
Where appropriate, the gene(s) for enhanced expression may be optimized for expression in the transformed plant. That is, the genes can be synthesized using plant-preferred codons corresponding to the plant of interest. Methods are available in the art for synthesizing plant-preferred genes. See, for example, U.S. Pat. Nos. 5,380,831, and 5,436,391, and Murray et al., 1989, Nucleic Acids Res. 17:477-498.
Additional sequence modifications are known to enhance gene expression in a cellular host. These include elimination of sequences encoding spurious polyadenylation signals, exon-intron splice site signals, transposon-like repeats, and other such well-characterized sequences that may be deleterious to gene expression. The G-C content of the sequence may be adjusted to levels average for a given cellular host, as calculated by reference to known genes expressed in the host cell. When desired, the sequence is modified to avoid predicted hairpin secondary mRNA structures. However, it is recognized that in the case of nucleotide sequences encoding the miRNA precursors, one or more hairpin and other secondary structures may be desired for proper processing of the precursor into an mature miRNA and/or for the functional activity of the miRNA in gene silencing.
The expression cassettes can additionally contain 5′ leader sequences in the expression cassette construct. Such leader sequences can act to enhance translation. Translation leaders are known in the art and include: picornavirus leaders, for example, EMCV leader (Encephalomyocarditis 5′ noncoding region) (Elroy-Stein et al., 1989, PNAS USA 86:6126-6130); potyvirus leaders, for example, TEV leader (Tobacco Etch Virus) (Allison et al., 1986); MDMV leader (Maize Dwarf Mosaic Virus); Virology 154:9-20), and human immunoglobulin heavy-chain binding protein (BiP), (Macejak et al., 1991, Nature 353:90-94); untranslated leader from the coat protein miRNA of alfalfa mosaic virus (AMV RNA 4) (Jobling et al., 1987, Nature 325:622-625); tobacco mosaic virus leader (TMV) (Gallie et al., 1989, Molecular Biology of RNA, ed. Cech (Liss, New York), pp. 237-256); and maize chlorotic mottle virus leader (MCMV) (Lommel et al., 1991, Virology 81:382-385). See also, Della-Cioppa et al., 1987, Plant Physiol. 84:965-968.
In preparing the expression cassette, the various DNA fragments can be manipulated, so as to provide for the DNA sequences in the proper orientation and, as appropriate, in the proper reading frame. Toward this end, adapters or linkers can be employed to join the DNA fragments or other manipulations may be involved to provide for convenient restriction sites, removal of superfluous DNA, removal of restriction sites, or the like. For this purpose, in vitro mutagenesis, primer repair, restriction, annealing, resubstitutions, e.g., transitions and transversions, may be involved.
An illustrative vector enoding CCA1 for overexpression in plants is described infra. Wang et al., 1998, Cell 93:1207-1217 also describes a vector for overexpressing CCA1 in plants. An illustrative vector enoding GLK1 for overexpression in plants is described infra. Further, Fitter et al., 2002, The Plant Journal 31:713-727 describe an insertion mutant in the GLK1 gene such that expression of GLK1 is disrupted.
6.5 Promoters and Other Regulatory Sequences
In the broad method of the invention, at least one nucleic acid sequence encoding CCA1, GLK1 or bZIP1 or a nucleic acid designed to disrupt expression of same is operably linked with a promoter, such as a leaf-preferred or leaf-specific promoter. It may be desirable to introduce more than one copy of a polynucleotide into a plant for enhanced expression. For example, multiple copies of a GLK1 polynucleotide would have the effect of increasing production of GLK1 even further in the plant. In specific embodiments, the GLK1 polynucleotide is expressed primarily or entirely in vegetative cells of the plant.
In general, promoters are found positioned 5′ (upstream) of the genes that they control. Thus, in the construction of promoter gene combinations, the promoter is preferably positioned upstream of the gene and at a distance from the transcription start site that approximates the distance between the promoter and the gene it controls in the natural setting. As is known in the art, some variation in this distance can be tolerated without loss of promoter function. Similarly, the preferred positioning of a regulatory element, such as an enhancer, with respect to a heterologous gene placed under its control reflects its natural position relative to the structural gene it naturally regulates. In certain specific embodiments, bZIP1 is under the control of a seed-specific promoter, and may optionally comprise other regulatory elements that result in constitutive or inducible expression of bZIP1.
Thus, the nucleic acid, in one embodiment, is operably linked to a gene expression sequence, which directs the expression of the nucleic acid within the cell. A “gene expression sequence,” as used herein, is any regulatory nucleotide sequence, such as a promoter sequence or promoter-enhancer combination, which facilitates the efficient transcription and translation of the nucleotide sequence to which it is operably linked. The gene expression sequence may, for example, be a eukaryotic promoter or a viral promoter, such as a constitutive or inducible promoter. Promoters and enhancers consist of short arrays of DNA sequences that interact specifically with cellular proteins involved in transcription, for instance, as discussed in Maniatis et al., 1987, Science 236:1237. Promoter and enhancer elements have been isolated from a variety of eukaryotic sources including genes in plant, yeast, insect and mammalian cells and viruses (analogous control elements, i.e., promoters, are also found in prokaryotes). In some embodiments, the nucleic acid is linked to a gene expression sequence which permits expression of the nucleic acid in a plant cell. A sequence which permits expression of the nucleic acid in a plant cell is one which is selectively active in the particular plant cell and thereby causes the expression of the nucleic acid in these cells. Those of ordinary skill in the art will be able to easily identify promoters that are capable of expressing a nucleic acid in a cell based on the type of plant cell.
A number of promoters can be used in the practice of the invention. The promoters can be selected based on the desired outcome. Generally, the nucleotide sequence and the modulator sequences can be combined with promoters of choice to alter gene expression if the target sequences in the tissue or organ of choice. Thus, the nucleotide sequence or modulator nucleotide sequence can be combined with constitutive, tissue-preferred, inducible, developmental, or other promoters for expression in plants depending upon the desired outcome.
The selection of a particular promoter and enhancer depends on what cell type is to be used and the mode of delivery. For example, a wide variety of promoters have been isolated from plants and animals, which are functional not only in the cellular source of the promoter, but also in numerous other plant species. There are also other promoters (e.g., viral and Ti-plasmid) which can be used. For example, these promoters include promoters from the Ti-plasmid, such as the octopine synthase promoter, the nopaline synthase promoter, the mannopine synthase promoter, and promoters from other open reading frames in the T-DNA, such as ORF7, etc. Promoters isolated from plant viruses include the 35S promoter from cauliflower mosaic virus. Promoters that have been isolated and reported for use in plants include ribulose-1,3-biphosphate carboxylase small subunit promoter, phaseolin promoter, etc. Thus, a variety of promoters and regulatory elements may be used in the expression vectors of the present invention.
Promoters useful in the compositions and methods provided herein include both natural constitutive and inducible promoters as well as engineered promoters. The CaMV promoters are examples of constitutive promoters. Other constitutive mammalian promoters include, but are not limited to, polymerase promoters as well as the promoters for the following genes: hypoxanthine phosphoribosyl transferase (“HPTR”), adenosine deaminase, pyruvate kinase, and alpha-actin.
Promoters useful as expression elements of the invention also include inducible promoters. Inducible promoters are expressed in the presence of an inducing agent. For example, a metallothionein promoter can be induced to promote transcription in the presence of certain metal ions. Other inducible promoters are known to those of ordinary skill in the art. The in vivo expression element can include, as necessary, 5′ non-transcribing and 5′ non-translating sequences involved with the initiation of transcription, and can optionally include enhancer sequences or upstream activator sequences.
For example, in some embodiments an inducible promoter is used to allow control of nucleic acid expression through the presentation of external stimuli (e.g., environmentally inducible promoters), as discussed below. Thus, the timing and amount of nucleic acid expression can be controlled in some cases. Non-limiting examples of expression systems, promoters, inducible promoters, environmentally inducible promoters, and enhancers are well known to those of ordinary skill in the art. Examples include those described in International Patent Application Publications WO 00/12714, WO 00/11175, WO 00/12713, WO 00/03012, WO 00/03017, WO 00/01832, WO 99/50428, WO 99/46976 and U.S. Pat. Nos. 6,028,250, 5,959,176, 5,907,086, 5,898,096, 5,824,857, 5,744,334, 5,689,044, and 5,612,472. A general descriptions of plant expression vectors and reporter genes can also be found in Gruber et al., 1993, “Vectors for Plant Transformation,” in Methods in Plant Molecular Biology & Biotechnology, Glich et al., Eds., p. 89-119, CRC Press.
For plant expression vectors, viral promoters that can be used in certain embodiments include the 35S RNA and 19S RNA promoters of CaMV (Brisson et al., Nature, 1984, 310:511; Odell et al., Nature, 1985, 313:810); the full-length transcript promoter from Figwort Mosaic Virus (FMV) (Gowda et al., 1989, J. Cell Biochem., 13D: 301) and the coat protein promoter to TMV (Takamatsu et al., 1987, EMBO J. 6:307). Alternatively, plant promoters such as the light-inducible promoter from the small subunit of ribulose bis-phosphate carboxylase (ssRUBISCO) (Coruzzi et al., 1984, EMBO J., 3:1671; Broglie et al., 1984, Science, 224:838); mannopine synthase promoter (Velten et al., 1984, EMBO J., 3:2723) nopaline synthase (NOS) and octopine synthase (OCS) promoters (carried on tumor-inducing plasmids of Agrobacterium tumefaciens) or heat shock promoters, e.g., soybean hsp17.5-E or hsp17.3-B (Gurley et al., 1986, Mol. Cell. Biol., 6:559; Severin et al., 1990, Plant Mol. Biol., 15:827) may be used. Exemplary viral promoters which function constitutively in eukaryotic cells include, for example, promoters from the simian virus, papilloma virus, adenovirus, human immunodeficiency virus, Rous sarcoma virus, cytomegalovirus, the long terminal repeats of Moloney leukemia virus and other retroviruses, and the thymidine kinase promoter of herpes simplex virus. Other constitutive promoters are known to those of ordinary skill in the art.
To be most useful, an inducible promoter should 1) provide low expression in the absence of the inducer; 2) provide high expression in the presence of the inducer; 3) use an induction scheme that does not interfere with the normal physiology of the plant; and 4) have no effect on the expression of other genes. Examples of inducible promoters useful in plants include those induced by chemical means, such as the yeast metallothionein promoter which is activated by copper ions (Mett et al., Proc. Natl. Acad. Sci., U.S.A., 90:4567, 1993); In2-1 and In2-2 regulator sequences which are activated by substituted benzenesulfonamides, e.g., herbicide safeners (Hershey et al., Plant Mol. Biol., 17:679, 1991); and the GRE regulatory sequences which are induced by glucocorticoids (Schena et al., Proc. Natl. Acad. Sci., U.S.A., 88:10421, 1991). Other promoters, both constitutive and inducible will be known to those of skill in the art.
A number of inducible promoters are known in the art. For resistance genes, a pathogen-inducible promoter can be utilized. Such promoters include those from pathogenesis-related proteins (PR proteins), which are induced following infection by a pathogen; e.g., PR proteins, SAR proteins, beta-1,3-glucanase, chitinase, etc. See, for example, Redolfi et al., 1983, Neth. J. Plant Pathol. 89:245-254; Uknes et al., 1992, Plant Cell 4:645-656; and Van Loon, 1985, Plant Mol. Virol. 4:111-116. Of particular interest are promoters that are expressed locally at or near the site of pathogen infection. See, for example, Marineau et al., 1987, Plant Mol. Biol. 9:335-342; Matton et al., 1989, Molecular Plant-Microbe Interactions 2:325-331; Somsisch et al., 1986, Proc. Natl. Acad. Sci. USA 83:2427-2430; Somsisch et al., 1988, Mol. Gen. Genet. 2:93-98; and Yang, 1996, Proc. Natl. Acad. Sci. USA 93:14972-14977. See also, Chen et al., 1996, Plant J. 10:955-966; Zhang et al., 1994, Proc. Natl. Acad. Sci. USA 91:2507-2511; Warner et al., 1993, Plant J. 3:191-201; Siebertz et al., 1989, Plant Cell 1:961-968; U.S. Pat. No. 5,750,386; Cordero et al., 1992, Physiol. Mol. Plant. Path. 41:189-200; and the references cited therein.
Additionally, as pathogens find entry into plants through wounds or insect damage, a wound-inducible promoter may be used in the DNA constructs of the invention. Such wound-inducible promoters include potato proteinase inhibitor (pin II) gene (Ryan, 1990, Ann. Rev. Phytopath. 28:425-449; Duan et al., 1996, Nature Biotechnology 14:494-498); wun1 and wun2, U.S. Pat. No. 5,428,148; win1 and win2 (Stanford et al., 1989, Mol. Gen. Genet. 215:200-208); systemin (McGurl et al., 1992, Science 225:1570-1573); WIPI (Rohmeier et al., 1993, Plant Mol. Biol. 22:783-792; Eckelkamp et al., 1993, FEBS Letters 323:73-76); MPI gene (Corderok et al., 1994, Plant J. 6(2):141-150); and the like. Such references are herein incorporated by reference.
Chemical-regulated promoters can be used to modulate the expression of a gene in a plant through the application of an exogenous chemical regulator. Depending upon the objective, the promoter may be a chemical-inducible promoter, where application of the chemical induces gene expression, or a chemical-repressible promoter, where application of the chemical represses gene expression. Chemical-inducible promoters are known in the art and include, but are not limited to, the maize In2-2 promoter, which is activated by benzenesulfonamide herbicide safeners, the maize GST promoter, which is activated by hydrophobic electrophilic compounds that are used as pre-emergent herbicides, and the tobacco PR-1 a promoter, which is activated by salicylic acid. Other chemical-regulated promoters of interest include steroid-responsive promoters (see, for example, the glucocorticoid-inducible promoter in Schena et al., 1991, Proc. Natl. Acad. Sci. USA 88:10421-10425 and McNellis et al., 1998, Plant J. 14(2):247-257) and tetramiR167e-inducible and tetramiR167e-repressible promoters (see, for example, Gatz et al., 1991, Mol. Gen. Genet. 227:229-237, and U.S. Pat. Nos. 5,814,618 and 5,789,156), herein incorporated by reference.
Where enhanced expression in particular tissues is desired, tissue-preferred promoters can be utilized. Tissue-preferred promoters include those described by Yamamoto et al., 1997, Plant J. 12(2):255-265; Kawamata et al., 1997, Plant Cell Physiol. 38(7):792-803; Hansen et al., 1997, Mol. Gen. Genet. 254(3):337-343; Russell et al., 1997, Transgenic Res. 6(2):157-168; Rinehart et al., 1996, Plant Physiol. 112(3):1331-1341; Van Camp et al., 1996, Plant Physiol. 112(2):525-535; Canevascini et al., 1996, Plant Physiol. 12(2):513-524; Yamamoto et al., 1994, Plant Cell Physiol. 35(5):773-778; Lam, 1994, Results Probl. Cell Differ. 20:181-196; Orozco et al., 1993, Plant Mol. Biol. 23(6): 1129-1138; Matsuoka et al., 1993, Proc Natl. Acad. Sci. USA 90(20):9586-9590; and Guevara-Garcia et al., 1993, Plant J 4(3):495-505.
The particular promoter selected should be capable of causing sufficient expression to result in the production of an effective amount of structural gene product in the transgenic plant, e.g., GLK1 to cause upregulation of genes such as GLN1.3 and increased nitrogen assimilation, biomass, overall plant growth or yield, and/or other phenotypes described herein, as compared to wild type. The promoters used in the vector constructs of the present invention may be modified, if desired, to affect their control characteristics. In certain embodiments, chimeric promoters can be used.
There are promoters known which limit expression to particular plant parts or in response to particular stimuli. One skilled in the art will know of many such plant part-specific promoters which would be useful in the present invention. In certain embodiments, to provide pericycle-specific expression, any of a number of promoters from genes in Arabidopsis can be used. In some embodiments, the promoter from one (or more) of the following genes may be used: (i) At1g11080, (ii) At3g60160, (iii) At1g24575, (iv) At3g45160, or (v) At1g23130. In specific embodiments, we will also use (vi) promoter elements from the GFP-marker line used in Gifford et al. (in preparation) (see also, Bonke et al., 2003, Nature 426, 181-6; Tian et al., 2004, Plant Physiol 135, 25-38). Several of the predicted genes have a number of potential orthologs in rice and poplar and thus are predicted that they will be applicable for use in crop species; (i) Os04g44410, Os10g39560, Os06g51370, Os02g42310, Os01g22980, Os05g06660, and Poptr1#568263, Poptr1#555534, Poptr1#365170; (ii) Os04g49900, Os04g49890, Os01g67580, and Poptr1#87573, Poptr1#80582, Poptr1#565079, Poptr1#99223.
Promoters used in the nucleic acid constructs of the present invention can be modified, if desired, to affect their control characteristics. For example, the CaMV 35S promoter may be ligated to the portion of the ssRUBISCO gene that represses the expression of ssRUBISCO in the absence of light, to create a promoter which is active in leaves but not in roots. The resulting chimeric promoter may be used as described herein. For purposes of this description, the phrase “CaMV 35S” promoter thus includes variations of CaMV 35S promoter, e.g., promoters derived by means of ligation with operator regions, random or controlled mutagenesis, etc. Furthermore, the promoters may be altered to contain multiple “enhancer sequences” to assist in elevating gene expression.
An efficient plant promoter that may be used in specific embodiments is an “overproducing” or “overexpressing” plant promoter. Overexpressing plant promoters that can be used in the compositions and methods provided herein include the promoter of the small sub-unit (“ss”) of the ribulose-1,5-biphosphate carboxylase from soybean (e.g., Berry-Lowe et al., 1982, J. Molecular & App. Genet., 1:483), and the promoter of the chorophyll a-b binding protein. These two promoters are known to be light-induced in eukaryotic plant cells. For example, see Cashmore, Genetic Engineering of plants: An Agricultural Perspective, p. 29-38; Coruzzi et al., 1983, J. Biol. Chem., 258:1399; and Dunsmuir et al., 1983, J. Molecular & App. Genet., 2:285.
The promoters and control elements of, e.g., SUCS (root nodules; broadbean; Kuster et al., 1993, Mol Plant Microbe Interact 6:507-14) for roots can be used in compositions and methods provided herein to confer tissue specificity.
In certain embodiment, two promoter elements can be used in combination, such as, for example, (i) an inducible element responsive to a treatment that can be provided to the plant prior to N-fertilizer treatment, and (ii) a plant tissue-specific expression element to drive expression in the specific tissue alone.
Any promoter of other expression element described herein or known in the art may be used either alone or in combination with any other promoter or other expression element described herein or known in the art. For example, promoter elements that confer tissue specific expression of a gene can be used with other promoter elements conferring constitutive or inducible expression.
6.6 Isolating Related Promoter Sequences
Promoter and promoter control elements that are related to those described in herein can also be used in the compositions and methods provided herein. Such related sequence can be isolated utilizing (a) nucleotide sequence identity; (b) coding sequence identity of related, orthologous genes; or (c) common function or gene products.
Relatives can include both naturally occurring promoters and non-natural promoter sequences. Non-natural related promoters include nucleotide substitutions, insertions or deletions of naturally-occurring promoter sequences that do not substantially affect transcription modulation activity. For example, the binding of relevant DNA binding proteins can still occur with the non-natural promoter sequences and promoter control elements of the present invention.
According to current knowledge, promoter sequences and promoter control elements exist as functionally important regions, such as protein binding sites, and spacer regions. These spacer regions are apparently required for proper positioning of the protein binding sites. Thus, nucleotide substitutions, insertions and deletions can be tolerated in these spacer regions to a certain degree without loss of function.
In contrast, less variation is permissible in the functionally important regions, since changes in the sequence can interfere with protein binding. Nonetheless, some variation in the functionally important regions is permissible so long as function is conserved.
The effects of substitutions, insertions and deletions to the promoter sequences or promoter control elements may be to increase or decrease the binding of relevant DNA binding proteins to modulate transcript levels of a polynucleotide to be transcribed. Effects may include tissue-specific or condition-specific modulation of transcript levels of the polypeptide to be transcribed. Polynucleotides representing changes to the nucleotide sequence of the DNA-protein contact region by insertion of additional nucleotides, changes to identity of relevant nucleotides, including use of chemically-modified bases, or deletion of one or more nucleotides are considered encompassed by the present invention.
Typically, related promoters exhibit at least 80% sequence identity, preferably at least 85%, more preferably at least 90%, and most preferably at least 95%, even more preferably, at least 96%, at least 97%, at least 98% or at least 99% sequence identity. Such sequence identity can be calculated by the algorithms and computers programs described above.
Usually, such sequence identity is exhibited in an alignment region that is at least 75% of the length of a sequence or corresponding full-length sequence of a promoter described herein; more usually at least 80%; more usually, at least 85%, more usually at least 90%, and most usually at least 95%, even more usually, at least 96%, at least 97%, at least 98% or at least 99% of the length of a sequence of a promoter described herein.
The percentage of the alignment length is calculated by counting the number of residues of the sequence in region of strongest alignment, e.g., a continuous region of the sequence that contains the greatest number of residues that are identical to the residues between two sequences that are being aligned. The number of residues in the region of strongest alignment is divided by the total residue length of a sequence of a promoter described herein. These related promoters may exhibit similar preferential transcription as those promoters described herein.
In certain embodiments, a promoter, such as a leaf-preferred or leaf-specific promoter, can be identified by sequence homology or sequence identity to any root specific promoter identified herein. In other embodiments, orthologous genes identified herein as leaf-specific genes (e.g., the same gene or different gene that if functionally equivalent) for a given species can be identified and the associated promoter can also be used in the compositions and methods provided herein. For example, using high, medium or low stringency conditions, standard promoter rules can be used to identify other useful promoters from orthologous genes for use in the compositions and methods provided herein. In specific embodiments, the orthologous gene is a gene expressed only or primarily in the root, such as pericycle cells.
Polynucleotides can be tested for activity by cloning the sequence into an appropriate vector, transforming plants with the construct and assaying for marker gene expression. Recombinant DNA constructs can be prepared, which comprise the polynucleotide sequences of the invention inserted into a vector suitable for transformation of plant cells. The construct can be made using standard recombinant DNA techniques (Sambrook et al., 1989) and can be introduced to the species of interest by Agrobacterium-mediated transformation or by other means of transformation as referenced below.
The vector backbone can be any of those typical in the art such as plasmids, viruses, artificial chromosomes, BACs, YACs and PACs and vectors of the sort described by (a) BAC: Shizuya et al., 1992, Proc. Natl. Acad. Sci. USA 89: 8794-8797; Hamilton et al., 1996, Proc. Natl. Acad. Sci. USA 93: 9975-9979; (b) YAC: Burke et al., 1987, Science 236:806-812; (c) PAC: Sternberg N. et al., 1990, Proc Natl Acad Sci USA. January; 87(1):103-7; (d) Bacteria-Yeast Shuttle Vectors: Bradshaw et al., 1995, Nucl Acids Res 23: 4850-4856; (e) Lambda Phage Vectors: Replacement Vector, e.g., Frischauf et al., 1983, J. Mol. Biol. 170: 827-842; or Insertion vector, e.g., Huynh et al., 1985, In: Glover N M (ed) DNA Cloning: A practical Approach, Vol. 1 Oxford: IRL Press; T-DNA gene fusion vectors: Walden et al., 1990, Mol Cell Biol 1: 175-194; and (g) Plasmid vectors: Sambrook et al., infra.
Typically, the construct comprises a vector containing a sequence of the present invention operationally linked to any marker gene. The polynucleotide was identified as a promoter by the expression of the marker gene. Although many marker genes can be used, Green Fluorescent Protein (GFP) is preferred. The vector may also comprise a marker gene that confers a selectable phenotype on plant cells. The marker may encode biocide resistance, particularly antibiotic resistance, such as resistance to kanamycin, G418, bleomycin, hygromycin, or herbicide resistance, such as resistance to chlorosulfuron or phosphinotricin (see below). Vectors can also include origins of replication, scaffold attachment regions (SARs), markers, homologous sequences, introns, etc.
6.7 Tissue or Cell-Type Preferential Transcription
The invention also provides a method of providing increased transcription of a nucleic acid sequence in a selected tissue, such as vegetative tissues, leaves, seeds, fruit, etc. The method comprises growing a plant having integrated in its genome a nucleic acid construct comprising, an exogeneous gene encoding CCA1, GLK1 or bZIP1, said gene operably associated with a tissue specific promoter, whereby transcription of said gene is increased (or decreased) in said selected tissue.
Specific promoters may be used in the compositions and methods provided herein. As used herein, “specific promoters” refers to a subset of promoters that have a high preference for modulating transcript levels in a specific tissue or organ or cell and/or at a specific time during development of an organism. By “high preference” is meant at least 3-fold, preferably 5-fold, more preferably at least 10-fold still more preferably at least 20-fold, 50-fold or 100-fold increase in transcript levels under the specific condition over the transcription under any other reference condition considered. Typical examples of temporal and/or tissue or organ specific promoters of plant origin that can be used in the compositions and methods of the present invention, include RCc2 and RCc3, promoters that direct root-specific gene transcription in rice (Xu et al., 1995, Plant Mol. Biol. 27:237 and TobRB27, a root-specific promoter from tobacco (Yamamoto et al., 1991, Plant Cell 3:371). Examples of tissue-specific promoters under developmental control include promoters that initiate transcription only in certain tissues or organs, such as roots
“Preferential transcription” is defined as transcription that occurs in a particular pattern of cell types or developmental times or in response to specific stimuli or combination thereof. Non-limitative examples of preferential transcription include: high transcript levels of a desired sequence in root tissues; detectable transcript levels of a desired sequence in certain cell types during embryogenesis; and low transcript levels of a desired sequence under drought conditions. Such preferential transcription can be determined by measuring initiation, rate, and/or levels of transcription.
Promoters and control elements providing preferential transcription in a root can modulate growth, metabolism, development, nutrient uptake, nitrogen fixation, or modulate energy and nutrient utilization in host cells or organisms. In a plant, for example, preferential modulation of genes, transcripts, and/or in a leaf, is useful (1) to modulate root size, shape, and development; (2) to modulate the number of roots, or root hairs; (3) to modulate mineral, fertilizer, or water uptake; (4) to modulate transport of nutrients; or (4) to modulate energy or nutrient usage in relation to other organs and tissues. Up-regulation and transcription down-regulation is useful for these applications. For instance, genes, transcripts, and/or polypeptides that increase growth, for example, may require up-regulation of transcription. In contrast, transcriptional down-regulation may be desired to inhibit nutrient usage in a root to be directed to the leaf instead, for instance.
Typically, promoter or control elements, which provide preferential transcription in cells, tissues, or organs of a root, produce transcript levels that are statistically significant as compared to other cells, organs or tissues. For preferential up-regulation of transcription, promoter and control elements produce transcript levels that are above background of the assay.
Root-preferred promoters are known and can be selected from the many available from the literature. See, for example, Hire et al., 1992, Plant Mol. Biol. 20(2): 207-218 (soybean root-preferred glutamine synthetase gene); Keller and Baumgartner, 1991, Plant Cell 3(10):1051-1061 (root-preferred control element in the GRP 1.8 gene of French bean); Sanger et al., 1990, Plant Mol. Biol. 14(3):433-443 (root-preferred promoter of the mannopine synthase (MAS) gene of Agrobacterium tumefaciens); Miao et al., 1991, Plant Cell 3(1):11-22 (full-length cDNA clone encoding cytosolic glutamine synthetase (GS), which is expressed in roots and root nodules of soybean). Bogusz et al., 1990, Plant Cell 2(7):633-641 (root-preferred promoters from hemoglobin genes from the nitrogen-fixing nonlegume Parasponia andersonii and the related non-nitrogen-fixing nonlegume Trema tomentosa). Leach and Aoyagi, 1991, Plant Science (Limerick) 79(1):69-76 (ro1C and ro1D root-inducing genes of Agrobacterium rhizogenes); Teeri et al., 1989, EMBO J. 8(2):343-350) (octopine synthase and TR2′ gene); (VfENOD-GRP3 gene promoter); Kuster et al., 1995, Plant Mol. Biol. 29(4):759-772 and Capana et al., 1994, Plant Mol. Biol. 25(4):681-691 ro1B promoter. See also U.S. Pat. Nos. 5,837,876; 5,750,386; 5,633,363; 5,459,252; 5,401,836; 5,110,732; and 5,023,179, root-specific glutamine synthetase (see Tingey et al., 1987, EMBO J., 6:1-9; Edwards et al., 1990, PNAS, 87:3439-3463). In addition, promoters of the above-listed orthologous genes in other plant species can be identified and used in the compositions and methods provided herein.
In specific embodiments, the compositions and methods provided herein use leaf-specific promoters operably associated to a nucleotide encoding bZIP1. In certain embodiments, the promoter is a constitutive or inducible promoter. In another specific embodiment, the compositions and methods provided herein use vegetative tissue-specific promoters operably associated to a nucleotide encoding CCA1 and/or GLK1. In certain embodiments, the promoter is a constitutive or inducible promoter.
6.8 Selectable Markers
Using any gene transfer technique, such as the above-listed techniques, an expression vector harboring the nucleic acid may be transformed into a cell to achieve temporary or prolonged expression. Any suitable expression system may be used, so long as it is capable of undergoing transformation and expressing of the precursor nucleic acid in the cell. In one embodiment, a pET vector (Novagen, Madison, Wis.), or a pBI vector (Clontech, Palo Alto, Calif.) is used as the expression vector. In some embodiments an expression vector further encoding a green fluorescent protein (“GFP”) is used to allow simple selection of transfected cells and to monitor expression levels. Non-limiting examples of such vectors include Clontech's “Living Colors Vectors” pEYFP and pEYFP-C.
The recombinant construct of the present invention may include a selectable marker for propagation of the construct. For example, a construct to be propagated in bacteria preferably contains an antibiotic resistance gene, such as one that confers resistance to kanamycin, tetracycline, streptomycin, or chloramphenicol. Suitable vectors for propagating the construct include plasmids, cosmids, bacteriophages or viruses, to name but a few.
In addition, the recombinant constructs may include plant-expressible selectable or screenable marker genes for isolating, identifying or tracking of plant cells transformed by these constructs. Selectable markers include, but are not limited to, genes that confer antibiotic resistances (e.g., resistance to kanamycin or hygromycin) or herbicide resistance (e.g., resistance to sulfonylurea, phosphinothricin, or glyphosate). Screenable markers include, but are not limited to, the genes encoding β-glucuronidase (Jefferson, 1987, Plant Molec Biol. Rep 5:387-405), luciferase (Ow et al., 1986, Science 234:856-859), B and C1 gene products that regulate anthocyanin pigment production (Goff et al., 1990, EMBO J. 9:2517-2522).
In some cases, a selectable marker may be included with the nucleic acid being delivered to the cell. As used herein, the term “selectable marker” refers to the use of a gene that encodes an enzymatic or other detectable activity (e.g., luminescence or fluorescence) that confers the ability to grow in medium lacking what would otherwise be an essential nutrient. A selectable marker may also confer resistance to an antibiotic or drug upon the cell in which the selectable marker is expressed. Selectable markers may be “dominant” in some cases; a dominant selectable marker encodes an enzymatic or other activity (e.g., luminescence or fluorescence) that can be detected in any cell or cell line.
Optionally, a selectable marker may be associated with the CCA1-, GLK1 or bZIP1-encoding nucleic acid. Preferably, the marker gene is an antibiotic resistance gene whereby the appropriate antibiotic can be used to select for transformed cells from among cells that are not transformed. Examples of suitable selectable markers include adenosine deaminase, dihydrofolate reductase, hygromycin-B-phosphotransferase, thymidine kinase, xanthine-guanine phospho-ribosyltransferase and amino-glycoside 3′-O-phosphotransferase II. Other suitable markers will be known to those of skill in the art.
6.9 Selection and Identification of Transformed Plants and Plant Cells
According to the present invention, desired plants may be obtained by engineering the disclosed gene constructs into a variety of plant cell types, including but not limited to, protoplasts, tissue culture cells, tissue and organ explants, pollens, embryos as well as whole plants. In specific embodiments, the gene constructs are engineered into leaves, preferably with the use of a leaf-specific promoter.
In an embodiment of the present invention, the engineered plant material is selected or screened for transformants (those that have incorporated or integrated the introduced gene construct(s)) following the approaches and methods described below. An isolated transformant may then be regenerated into a plant. Alternatively, the engineered plant material may be regenerated into a plant or plantlet before subjecting the derived plant or plantlet to selection or screening for the marker gene traits. Procedures for regenerating plants from plant cells, tissues or organs, either before or after selecting or screening for marker gene(s), are well known to those skilled in the art.
A transformed plant cell, callus, tissue or plant may be identified and isolated by selecting or screening the engineered plant material for traits encoded by the marker genes present on the transforming DNA. For instance, selection may be performed by growing the engineered plant material on media containing inhibitory amount of the antibiotic or herbicide to which the transforming gene construct confers resistance. Further, transformed plants and plant cells may also be identified by screening for the activities of any visible marker genes (e.g., the β-glucuronidase, luciferase, B or C1 genes) that may be present on the recombinant nucleic acid constructs of the present invention. Such selection and screening methodologies are well known to those skilled in the art.
Physical and biochemical methods also may be also to identify plant or plant cell transformants containing the gene constructs of the present invention. These methods include but are not limited to: 1) Southern analysis or PCR amplification for detecting and determining the structure of the recombinant DNA insert; 2) Northern blot, 51 RNase protection, primer-extension or reverse transcriptase-PCR amplification for detecting and examining RNA transcripts of the gene constructs; 3) enzymatic assays for detecting enzyme or ribozyme activity, where such gene products are encoded by the gene construct; 4) protein gel electrophoresis, Western blot techniques, immunoprecipitation, or enzyme-linked immunoassays, where the gene construct products are proteins. Additional techniques, such as in situ hybridization, enzyme staining, and immunostaining, also may be used to detect the presence or expression of the recombinant construct in specific plant organs and tissues. The methods for doing all these assays are well known to those skilled in the art.
6.10 Screening of Transformed Plants for Those with Improved Agronomic Traits
According to the present invention, to obtain plants with improved agronomic characteristics, the transformed plants may be screened for those exhibiting the desired physiological alteration. Alternatively, the transformed plants may be directly screened for those exhibiting the desired agronomic changes. A plant with the desired improvement can be isolated by screening the engineered plants for altered expression pattern or level of CCA1, GLK1 and/or bZIP1, or downstream gene products such as GLN1.3 or ASN1. A plant can also be screened for nutrient uptake, overall increased plant growth rate, enhanced vegetative yield, improved reproductive yields, increased levels of glutamine or asparagine, or increased nitrogen usage or storage. The screening of the engineered plants can involve Southern analysis to confirm the presence and number of transgene insertions; Northern analysis, RNase protection, primer extension, reverse transcriptase/PCR and the like to measure mRNA levels; measuring the amino acid composition, free amino acid pool or total nitrogen content of various plant tissues; measuring growth rates in terms of fresh weight gains over time; or measuring plant yield in terms of total dry weight and/or total seed weight, or a combination of any of the above methods. The procedures and methods for examining these parameters are well known to those skilled in the art.
In other embodiments, the screening of the transformed plants may be for improved agronomic characteristics (e.g., faster growth, greater vegetative or reproductive yields, or improved protein contents, etc.), as compared to unengineered progenitor plants, when cultivated under growth conditions (i.e., cultivated using soils or media containing or receiving sufficient amounts of nitrogen nutrients to sustain healthy plant growth).
Plants exhibiting increased growth and/or yield as compared with wild-type plants can be selected by visual observation, methods provided in the Examples, or other methods known in the art.
A “plant capable of increased yield” refers to a plant that can be induced to express its endogenous CCA1, GLK1 and/or bZIP1 gene to achieve increased yield. The term “promoter inducing amount” refers to that amount of an agent necessary to elevate such gene expression above such expression in a plant cell not contacted with the agent, by stimulating the endogenous promoter. For example, a transcription factor or a chemical agent may be used to elevate gene expression from native or chimeric CCA1, GLK1 and/or bZIP1 promoter, thus inducing the promoter and gene expression.
6.11 Cells
Optionally, germ line cells may be used in the methods described herein rather than, or in addition to, somatic cells. The term “germ line cells” refers to cells in the plant organism which can trace their eventual cell lineage to either the male or female reproductive cell of the plant. Other cells, referred to as “somatic cells” are cells which give rise to leaves, roots and vascular elements which, although important to the plant, do not directly give rise to gamete cells. Somatic cells, however, also may be used. With regard to callus and suspension cells which have somatic embryogenesis, many or most of the cells in the culture have the potential capacity to give rise to an adult plant. If the plant originates from single cells or a small number of cells from the embryogenic callus or suspension culture, the cells in the callus and suspension can therefore be referred to as germ cells. In the case of immature embryos which are prepared for treatment by the methods described herein, certain cells in the apical meristem region of the plant have been shown to produce a cell lineage which eventually gives rise to the female and male reproductive organs. With many or most species, the apical meristem is generally regarded as giving rise to the lineage that eventually will give rise to the gamete cells. An example of a non-gamete cell in an embryo would be the first leaf primordia in corn which is destined to give rise only to the first leaf and none of the reproductive structures.
6.12 Plant Regeneration
Following transformation, a plant may be regenerated, e.g., from single cells, callus tissue or leaf discs, as is standard in the art. Almost any plant can be entirely regenerated from cells, tissues, and organs of the plant. Available techniques are reviewed in Vasil et al., 1984, in Cell Culture and Somatic Cell Genetics of Plants, Vols. I, II, and III, Laboratory Procedures and Their Applications (Academic Press); and Weissbach et al., 1989, Methods For Plant Mol. Biol.
The transformed plants may then be grown, and either pollinated with the same transformed strain or different strains, and the resulting hybrid having expression of the desired phenotypic characteristic identified. Two or more generations may be grown to ensure that expression of the desired phenotypic characteristic is stably maintained and inherited and then seeds harvested to ensure expression of the desired phenotypic characteristic has been achieved.
Normally, a plant cell is regenerated to obtain a whole plant from the transformation process. The term “growing” or “regeneration” as used herein means growing a whole plant from a plant cell, a group of plant cells, a plant part (including seeds), or a plant piece (e.g., from a protoplast, callus, or tissue part).
Regeneration from protoplasts varies from species to species of plants, but generally a suspension of protoplasts is first made. In certain species, embryo formation can then be induced from the protoplast suspension. The culture media will generally contain various amino acids and hormones, necessary for growth and regeneration. Examples of hormones utilized include auxins and cytokinins. Efficient regeneration will depend on the medium, on the genotype, and on the history of the culture. If these variables are controlled, regeneration is reproducible.
Regeneration also occurs from plant callus, explants, organs or parts. Transformation can be performed in the context of organ or plant part regeneration (see Methods in Enzymology, Vol. 118 and Klee et al., Annual Review of Plant Physiology, 38:467, 1987). Utilizing the leaf disk-transformation-regeneration method of Horsch et al., Science, 227:1229, 1985, disks are cultured on selective media, followed by shoot formation in about 2-4 weeks. Shoots that develop are excised from calli and transplanted to appropriate root-inducing selective medium. Rooted plantlets are transplanted to soil as soon as possible after roots appear. The plantlets can be repotted as required, until reaching maturity.
In vegetatively propagated crops, the mature transgenic plants are propagated by utilizing cuttings or tissue culture techniques to produce multiple identical plants. Selection of desirable transgenics is made and new varieties are obtained and propagated vegetatively for commercial use.
In seed propagated crops, mature transgenic plants can be self crossed to produce a homozygous inbred plant. The resulting inbred plant produces seed containing the newly introduced foreign gene(s). These seeds can be grown to produce plants that would produce the selected phenotype, e.g., increased lateral root growth, uptake of nutrients, overall plant growth and/or vegetative or reproductive yields.
Parts obtained from the regenerated plant, such as flowers, seeds, leaves, branches, fruit, and the like are included in the invention, provided that these parts comprise cells comprising the isolated nucleic acid of the present invention. Progeny and variants, and mutants of the regenerated plants are also included within the scope of the invention, provided that these parts comprise the introduced nucleic acid sequences. Transgenic plants expressing the selectable marker can be screened for transmission of the nucleic acid of the present invention by, for example, standard immunoblot and DNA detection techniques. Transgenic lines are also typically evaluated on levels of expression of the heterologous nucleic acid. Expression at the RNA level can be determined initially to identify and quantitate expression-positive plants. Standard techniques for RNA analysis can be employed and include PCR amplification assays using oligonucleotide primers designed to amplify only the heterologous RNA templates and solution hybridization assays using heterologous nucleic acid-specific probes. The RNA-positive plants can then analyzed for protein expression by Western immunoblot analysis using the specifically reactive antibodies of the present invention. In addition, in situ hybridization and immunocytochemistry according to standard protocols can be done using heterologous nucleic acid specific polynucleotide probes and antibodies, respectively, to localize sites of expression within transgenic tissue. Generally, a number of transgenic lines are usually screened for the incorporated nucleic acid to identify and select plants with the most appropriate expression profiles.
A preferred embodiment is a transgenic plant that is homozygous for the added heterologous nucleic acid; i.e., a transgenic plant that contains two added nucleic acid sequences, one gene at the same locus on each chromosome of a chromosome pair. A homozygous transgenic plant can be obtained by sexually mating (selfing) a heterozygous transgenic plant that contains a single added heterologous nucleic acid, germinating some of the seed produced and analyzing the resulting plants produced for altered expression of a polynucleotide of the present invention relative to a control plant (i.e., native, non-transgenic). Back-crossing to a parental plant and out-crossing with a non-transgenic plant are also contemplated.
Transformed plant cells which are derived by any of the above transformation techniques can be cultured to regenerate a whole plant which possesses the transformed genotype. Such regeneration techniques often rely on manipulation of certain phytohormones in a tissue culture growth medium. For transformation and regeneration of maize see, Gordon-Kamm et al., 1990, The Plant Cell, 2:603-618.
Plants cells transformed with a plant expression vector can be regenerated, e.g., from single cells, callus tissue or leaf discs according to standard plant tissue culture techniques. It is well known in the art that various cells, tissues, and organs from almost any plant can be successfully cultured to regenerate an entire plant. Plant regeneration from cultured protoplasts is described in Evans et al., 1983, Protoplasts Isolation and Culture, Handbook of Plant Cell Culture, Macmillan Publishing Company, New York, pp. 124-176; and Binding, Regeneration of Plants, Plant Protoplasts, 1985, CRC Press, Boca Raton, pp. 21-73.
The regeneration of plants containing the foreign gene introduced by Agrobacterium from leaf explants can be achieved as described by Horsch et al., 1985, Science, 227:1229-1231. In this procedure, transformants are grown in the presence of a selection agent and in a medium that induces the regeneration of shoots in the plant species being transformed as described by Fraley et al., 1983, Proc. Natl. Acad. Sci. (U.S.A.), 80:4803. This procedure typically produces shoots within two to four weeks and these transformant shoots are then transferred to an appropriate root-inducing medium containing the selective agent and an antibiotic to prevent bacterial growth. Transgenic plants of the present invention may be fertile or sterile.
The regeneration of plants from either single plant protoplasts or various explants is well known in the art. See, for example, Methods for Plant Molecular Biology, A. Weissbach and H. Weissbach, eds., 1988, Academic Press, Inc., San Diego, Calif. This regeneration and growth process includes the steps of selection of transformant cells and shoots, rooting the transformant shoots and growth of the plantlets in soil. For maize cell culture and regeneration see generally, The Maize Handbook, Freeling and Walbot, Eds., 1994, Springer, New York 1994; Corn and Corn Improvement, 3rd edition, Sprague and Dudley Eds., 1988, American Society of Agronomy, Madison, Wis.
6.13 Plants and Plant Cells
Also provided herein are a plant cell having the nucleotide sequence constructs of the invention. A further aspect of the present invention provides a method of making such a plant cell involving introduction of a vector including the construct into a plant cell. For integration of the construct into the plant genome, such introduction will be followed by recombination between the vector and the plant cell genome to introduce the sequence of nucleotides into the genome. RNA encoded by the introduced nucleic acid construct may then be transcribed in the cell and descendants thereof, including cells in plants regenerated from transformed material. A gene stably incorporated into the genome of a plant is passed from generation to generation to descendants of the plant, so such descendants should show the desired phenotype.
In certain embodiments, a plant cell comprises a GLK1 nucleotide sequence operably associated with a vegetative tissue specific promoter, which is optionally a constitutive or inducible promoter. In other embodiments, a plant cell comprises multiple copies of a GLK1 operably associated with a vegetative tissue specific promoter. In specific embodiments provided herein are plants (and plant cells thereof) that overexpress, contitutionally express and/or inducibly express GLK1 in the vegetative tissues of the plant, as compared to other tissues in the plant and/or as compared to a wild type plant.
The present invention also provides a plant comprising a plant cell as disclosed. Transformed seeds and plant parts are also encompassed.
In addition to a plant, the present invention provides any clone of such a plant, seed, selfed or hybrid progeny and descendants, and any part of any of these, such as cuttings, seed. The invention provides any plant propagule, that is any part which may be used in reproduction or propagation, sexual or asexual, including cuttings, seed and so on. Also encompassed by the invention is a plant which is a sexually or asexually propagated off-spring, clone or descendant of such a plant, or any part or propagule of said plant, off-spring, clone or descendant. Plant extracts and derivatives are also provided.
Any species of woody, ornamental or decorative, crop or cereal, fruit or vegetable plant, and algae (e.g., Chlamydomonas reinhardtii) may be used in the compositions and methods provided herein. Non-limiting examples of plants include plants from the genus Arabidopsis or the genus Oryza. Other examples include plants from the genuses Acorus, Aegilops, Allium, Amborella, Antirrhinum, Apium, Arachis, Beta, Betula, Brassica, Capsicum, Ceratopteris, Citrus, Cryptomeria, Cycas, Descurainia, Eschscholzia, Eucalyptus, Glycine, Gossypium, Hedyotis, Helianthus, Hordeum, Ipomoea, Lactuca, Linum, Liriodendron, Lotus, Lupinus, Lycopersicon, Medicago, Mesembryanthemum, Nicotiana, Nuphar, Pennisetum, Persea, Phaseolus, Physcomitrella, Picea, Pinus, Poncirus, Populus, Prunus, Robinia, Rosa, Saccharum, Schedonorus, Secale, Sesamum, Solanum, Sorghum, Stevia, Thellungiella, Theobroma, Triphysaria, Triticum, Vitis, Zea, or Zinnia.
Plants included in the invention are any plants amenable to transformation techniques, including gymnosperms and angiosperms, both monocotyledons and dicotyledons.
Examples of monocotyledonous angiosperms include, but are not limited to, asparagus, field and sweet corn, barley, wheat, rice, sorghum, onion, pearl millet, rye and oats and other cereal grains.
Examples of dicotyledonous angiosperms include, but are not limited to tomato, tobacco, cotton, rapeseed, field beans, soybeans, peppers, lettuce, peas, alfalfa, clover, cole crops or Brassica oleracea (e.g., cabbage, broccoli, cauliflower, brussel sprouts), radish, carrot, beets, eggplant, spinach, cucumber, squash, melons, cantaloupe, sunflowers and various ornamentals.
Examples of woody species include poplar, pine, sequoia, cedar, oak, etc.
Still other examples of plants include, but are not limited to, wheat, cauliflower, tomato, tobacco, corn, petunia, trees, etc.
In certain embodiments, plants of the present invention are crop plants (for example, cereals and pulses, maize, wheat, potatoes, tapioca, rice, sorghum, millet, cassaya, barley, pea, and other root, tuber, or seed crops. Exemplary cereal crops used in the compositions and methods of the invention include, but are not limited to, any species of grass, or grain plant (e.g., barley, corn, oats, rice, wild rice, rye, wheat, millet, sorghum, triticale, etc.), non-grass plants (e.g., buckwheat flax, legumes or soybeans, etc.). Grain plants that provide seeds of interest include oil-seed plants and leguminous plants. Other seeds of interest include grain seeds, such as corn, wheat, barley, rice, sorghum, rye, etc. Oil seed plants include cotton, soybean, safflower, sunflower, Brassica, maize, alfalfa, palm, coconut, etc. Other important seed crops are oil-seed rape, sugar beet, maize, sunflower, soybean, and sorghum. Leguminous plants include beans and peas. Beans include guar, locust bean, fenugreek, soybean, garden beans, cowpea, mungbean, lima bean, fava bean, lentils, chickpea, etc.
Horticultural plants to which the present invention may be applied may include lettuce, endive, and vegetable brassicas including cabbage, broccoli, and cauliflower, and carnations and geraniums. The present invention may also be applied to tobacco, cucurbits, carrot, strawberry, sunflower, tomato, pepper, chrysanthemum, poplar, eucalyptus, and pine.
The present invention may be used for transformation of other plant species, including, but not limited to, corn (Zea mays), canola (Brassica napus, Brassica rapa ssp.), alfalfa (Medicago sativa), rice (Oryza sativa), rye (Secale cereale), sorghum (Sorghum bicolor, Sorghum vulgare), sunflower (Helianthus annuus), wheat (Triticum aestivum), soybean (Glycine max), tobacco (Nicotiana tabacum, Nicotiana benthamiana), potato (Solanum tuberosum), peanuts (Arachis hypogaea), cotton (Gossypium hirsutum), sweet potato (Ipomoea batatus), cassaya (Manihot esculenta), coffee (Coffea spp.), coconut (Cocos nucifera), pineapple (Ananas comosus), citrus trees (Citrus spp.), cocoa (Theobroma cacao), tea (Camellia sinensis), banana (Musa spp.), avocado (Persea americana), fig (Ficus casica), guava (Psidium guajava), mango (Mangifera indica), olive (Olea europaea), papaya (Carica papaya), cashew (Anacardium occidentale), macadamia (Macadamia integrifolia), almond (Prunus amygdalus), sugar beets (Beta vulgaris), oats, barley, Arabidopsis spp., vegetables, ornamentals, and conifers.
6.14 Cultivation
Methods of cultivation of plants are well known in the art. For example, for the cultivation of wheat see Alcoz et al., 1993, Agronomy Journal 85:1198-1203; Rao and Dao, 1992, J. Am. Soc. Agronomy 84:1028-1032; Howard and Lessman, 1991, Agronomy Journal 83:208-211; for the cultivation of corn see Tollenear et al., 1993, Agronomy Journal 85:251-255; Straw et al., Tennessee Farm and Home Science: Progress Report, Spring 1993, 166:20-24; Miles, S. R., 1934, J. Am. Soc. Agronomy 26:129-137; Dara et al., 1992, J. Am. Soc. Agronomy 84:1006-1010; Binford et al., 1992, Agronomy Journal 84:53-59; for the cultivation of soybean see Chen et al., 1992, Canadian Journal of Plant Science 72:1049-1056; Wallace et al., 1990, Journal of Plant Nutrition 13:1523-1537; for the cultivation of rice see Oritani and Yoshida, 1984, Japanese Journal of Crop Science 53:204-212; for the cultivation of linseed see Diepenbrock and Porksen, 1992, Industrial Crops and Products 1:165-173; for the cultivation of tomato see Grubinger et al., 1993, Journal of the American Society for Horticultural Science 118:212-216; Cerne, M., 1990, Acta Horticulture 277:179-182; for the cultivation of pineapple see Magistad et al., 1932, J. Am. Soc. Agronomy 24:610-622; Asoegwu, S, N., 1988, Fertilizer Research 15:203-210; Asoegwu, S, N., 1987, Fruits 42:505-509; for the cultivation of lettuce see Richardson and Hardgrave, 1992, Journal of the Science of Food and Agriculture 59:345-349; for the cultivation of mint see Munsi, P. S., 1992, Acta Horticulturae 306:436-443; for the cultivation of camomile see Letchamo, W., 1992, Acta Horticulturae 306:375-384; for the cultivation of tobacco see Sisson et al., 1991, Crop Science 31:1615-1620; for the cultivation of potato see Porter and Sisson, 1991, American Potato Journal, 68:493-505; for the cultivation of brassica crops see Rahn et al., 1992, Conference “Proceedings, second congress of the European Society for Agronomy” Warwick Univ., p. 424-425; for the cultivation of banana see Hegde and Srinivas, 1991, Tropical Agriculture 68:331-334; Langenegger and Smith, 1988, Fruits 43:639-643; for the cultivation of strawberries see Human and Kotze, 1990, Communications in Soil Science and Plant Analysis 21:771-782; for the cultivation of songhum see Mahalle and Seth, 1989, Indian Journal of Agricultural Sciences 59:395-397; for the cultivation of plantain see Anjorin and Obigbesan, 1985, Conference “International Cooperation for Effective Plantain and Banana Research” Proceedings of the third meeting. Abidjan, Ivory Coast, p. 115-117; for the cultivation of sugar cane see Yadav, R. L., 1986, Fertiliser News 31:17-22; Yadav and Sharma, 1983, Indian Journal of Agricultural Sciences 53:38-43; for the cultivation of sugar beet see Draycott et al., 1983, Conference “Symposium Nitrogen and Sugar Beet” International Institute for Sugar Beet Research—Brussels Belgium, p. 293-303. See also Goh and Haynes, 1986, “Nitrogen and Agronomic Practice” in Mineral Nitrogen in the Plant-Soil System, Academic Press, Inc., Orlando, Fla., p. 379-468; Engelstad, O. P., 1985, Fertilizer Technology and Use, Third Edition, Soil Science Society of America, p. 633; Yadav and Sharmna, 1983, Indian Journal of Agricultural Sciences, 53:3-43.
6.15 Products of Transgenic Plants
Engineered plants exhibiting the desired physiological and/or agronomic changes can be used directly in agricultural production.
Thus, provided herein are products derived from the transgenic plants or methods of producing transgenic plants provided herein. In certain embodiments, the products are commercial products. Some non-limiting example include genetically engineered trees for e.g., the production of pulp, paper, paper products or lumber; tobacco, e.g., for the production of cigarettes, cigars, or chewing tobacco; crops, e.g., for the production of fruits, vegetables and other food, including grains, e.g., for the production of wheat, bread, flour, rice, corn; and canola, sunflower, e.g., for the production of oils or biofuels.
In certain embodiments, commercial products are derived from a genetically engineered (e.g., comprising overexpression of GLK1 in the vegetative tissues of the plant) species of woody, ornamental or decorative, crop or cereal, fruit or vegetable plant, and algae (e.g., Chlamydomonas reinhardtii), which may be used in the compositions and methods provided herein. Non-limiting examples of plants include plants from the genus Arabidopsis or the genus Oryza. Other examples include plants from the genuses Acorus, Aegilops, Allium, Amborella, Antirrhinum, Apium, Arachis, Beta, Betula, Brassica, Capsicum, Ceratopteris, Citrus, Cryptomeria, Cycas, Descurainia, Eschscholzia, Eucalyptus, Glycine, Gossypium, Hedyotis, Helianthus, Hordeum, Ipomoea, Lactuca, Linum, Liriodendron, Lotus, Lupinus, Lycopersicon, Medicago, Mesembryanthemum, Nicotiana, Nuphar, Pennisetum, Peryea, Phaseolus, Physcomitrella, Picea, Pinus, Poncirus, Populus, Prunus, Robinia, Rosa, Saccharum, Schedonorus, Secale, Sesamum, Solanum, Sorghum, Stevia, Thellungiella, Theobroma, Triphysaria, Triticum, Vitis, Zea, or Zinnia.
In some embodiments, commercial products are derived from a genetically engineered gymnosperms and angiosperms, both monocotyledons and dicotyledons. Examples of monocotyledonous angiosperms include, but are not limited to, asparagus, field and sweet corn, barley, wheat, rice, sorghum, onion, pearl millet, rye and oats and other cereal grains. Examples of dicotyledonous angiosperms include, but are not limited to tomato, tobacco, cotton, rapeseed, field beans, soybeans, peppers, lettuce, peas, alfalfa, clover, cole crops or Brassica oleracea (e.g., cabbage, broccoli, cauliflower, brussel sprouts), radish, carrot, beets, eggplant, spinach, cucumber, squash, melons, cantaloupe, sunflowers and various ornamentals.
In certain embodiments, commercial products are derived from a genetically engineered (e.g., comprising overexpression of bZIP1 in the leaves or seeds of the plant) woody species, such as poplar, pine, sequoia, cedar, oak, etc.
In other embodiments, commercial products are derived from a genetically engineered (e.g., comprising overexpression of CCA1 and GLK1 in the vegetative tissues of the plant) plant including, but are not limited to, wheat, cauliflower, tomato, tobacco, corn, petunia, trees, etc.
In certain embodiments, commercial products are derived from a genetically engineered crop plants, for example, cereals and pulses, maize, wheat, potatoes, tapioca, rice, sorghum, millet, cassaya, barley, pea, and other root, tuber, or seed crops. In one embodiment, commercial products are derived from a genetically engineered (e.g., comprising overexpression of CCA1 and GLK1 and underexpression of bZIP1 in the vegetative tissues of the plant) cereal crops, including, but are not limited to, any species of grass, or grain plant (e.g., barley, corn, oats, rice, wild rice, rye, wheat, millet, sorghum, triticale, etc.), non-grass plants (e.g., buckwheat flax, legumes or soybeans, etc.). In another embodiments, commercial products are derived from a genetically engineered (e.g., comprising overexpression of bZIP1 and optionally underexpression of CCA1 and/or GLK1 in leaf or seed tissue of the plant) grain plants that provide seeds of interest, oil-seed plants and leguminous plants. In other embodiments, commercial products are derived from a genetically engineered grain seed plants, such as corn, wheat, barley, rice, sorghum, rye, etc. In yet other embodiments, commercial products are derived from a genetically engineered (e.g., comprising overexpression of bZIP1 and optionally underexpression of CCA1 and/or GLK1 in leaf or seed tissue of the plant) oil seed plants, such as cotton, soybean, safflower, sunflower, Brassica, maize, alfalfa, palm, coconut, etc. In certain embodiments, commercial products are derived from a genetically engineered oil-seed rape, sugar beet, maize, sunflower, soybean, or sorghum. In some embodiments, commercial products are derived from a genetically engineered leguminous plants, such as beans and peas (e.g., guar, locust bean, fenugreek, soybean, garden beans, cowpea, mungbean, lima bean, fava bean, lentils, chickpea, etc.)
In certain embodiments, commercial products are derived from a genetically engineered horticultural plant of the present invention, such as lettuce, endive, and vegetable brassicas including cabbage, broccoli, and cauliflower, and carnations and geraniums; tomato, tobacco, cucurbits, carrot, strawberry, sunflower, tomato, pepper, chrysanthemum, poplar, eucalyptus, and pine.
In still other embodiments, commercial products are derived from a genetically engineered corn (Zea mays), canola (Brassica napus, Brassica rapa ssp.), alfalfa (Medicago sativa), rice (Oryza sativa), rye (Secale cereale), sorghum (Sorghum bicolor, Sorghum vulgare), sunflower (Helianthus annuus), wheat (Triticum aestivum), soybean (Glycine max), tobacco (Nicotiana tabacum, Nicotiana benthamiana), potato (Solanum tuberosum), peanuts (Arachis hypogaea), cotton (Gossypium hirsutum), sweet potato (Ipomoea batatus), cassaya (Manihot esculenta), coffee (Coffea spp.), coconut (Cocos nucifera), pineapple (Ananas comosus), citrus trees (Citrus spp.), cocoa (Theobroma cacao), tea (Camellia sinensis), banana (Musa spp.), avocado (Peryea americana), fig (Ficus casica), guava (Psidium guajava), mango (Mangifera indica), olive (Olea europaea), papaya (Carica papaya), cashew (Anacardium occidentale), macadamia (Macadamia integrifolia), almond (Prunus amygdalus), sugar beets (Beta vulgaris), oats, barley, Arabidopsis spp., vegetables, ornamentals, and conifers.
6.16 Kits
In one aspect, the present invention provides any of the above-mentioned compositions in kits, optionally including instructions for use of the composition e.g., for the overexpression or underexpression of CCA1, GLK1 or bZIP1. The “kit” typically defines a package including one or more compositions of the invention and the instructions, and/or analogs, derivatives, or functionally equivalent compositions thereof. Thus, for example, the kit can include a description of use of the composition for participation in any technique associated in the overexpression or underexpression of genes. The kit can include a description of use of the compositions as discussed herein. Instructions also may be provided for use of the composition in any suitable technique as previously described. The instructions may be of any form provided in connection with the composition.
The kits described herein may also contain one or more containers, which may contain the inventive composition and other ingredients as previously described. The kits also may contain instructions for mixing, diluting, and/or administrating the compositions in some cases. The kits also can include other containers with one or more solvents, surfactants, preservative and/or diluents (e.g., normal saline (0.9% NaCl), or 5% dextrose) as well as containers for mixing, diluting and/or administrating the compositions.
The compositions of the kit may be provided as any suitable form, for example, as liquid solutions or as dried powders. When the composition provided is a dry powder, the composition may be reconstituted by the addition of a suitable solvent, which may also be provided. In embodiments where liquid forms of the composition are used, the liquid form may be concentrated or ready to use. The solvent will depend on the active compound(s) within the composition. Suitable solvents are well known, for example as previously described, and are available in the literature.
The invention also involves, in another aspect, promotion of the overexpression of a master regulatory gene of the present invention, e.g., CCA1/GLK1/bZIP1, according to any of the systems or methods described herein. As used herein, “promoted” includes all methods of doing business including methods of education, hospital and other clinical instruction, pharmaceutical industry activity including pharmaceutical sales, and any advertising or other promotional activity including written, oral and electronic communication of any form, associated with compositions of the invention. “Instructions” can define a component of promotion, and typically involve written instructions on or associated with packaging of compositions of the invention. Instructions also can include any oral or electronic instructions provided in any manner.
7. EXAMPLE
7.1 Introduction
Nitrogen (N) is an essential nutrient and a metabolic signal that is sensed and transduced resulting in the control of gene expression in plants. Studies using nitrate reductase (NR) mutant plants, have shown that nitrate can serve as a metabolic signal for inorganic N that regulates gene expression in Arabidopsis thaliana and other plant species (Wang et al., 2004, Plant Physiol 136:2512-2522; Wang et al., 2003, Plant Physiol 132:556-567; Forde, 2002, Ann. Rev. Plant Biology 53:203-224; Scheible, et al., 2004, Plant Physiol 136:2483-2499). There is also ample though less direct evidence that the assimilated forms of N such as Glu or Gln may also serve as signals that regulate gene expression in plants (Rawat et al., 1999, Plant J 19:143-152; Oliveira et al., 1999, Plant Physiol 121:301-310). The ability of plants to sense and respond to levels of inorganic and organic N metabolites provides a mechanism to balance the availability of organic N resources within the plant with the need for N uptake. Because nitrate uptake, reduction and its assimilation into organic form require energy, a mechanism that activates this N assimilatory pathway based on sensing levels of organic N available in the plant is an efficient way to control N-use efficiency (Forde, 2002, Ann. Rev. Plant Biology 53:203-224). In plants, the transcription of genes involved in the uptake and assimilation of inorganic N is induced when levels of organic N are low. Conversely, the uptake and reduction of inorganic N are shut off when levels of organic N are high (reviewed in Scheible, et al., 1997, Plant Cell 9:783-798).
Recent microarray studies have shown that nitrate can cause changes in the expression of a large number of genes in Arabidopsis (Wang et al., 2004, Plant Physiol 136:2512-2522; Wang et al., 2003, Plant Physiol 132:556-567). Treatment of Arabidopsis seedlings with low levels of nitrate has been shown to increase the levels of mRNA for hundreds of genes within minutes of exposure. The nitrate-responsive genes include nitrate transporters, NR and nitrite reductase, putative transcription factors, stress responses genes, as well as genes whose products play roles in glycolysis, iron metabolism, and sulfate uptake (Wang et al., 2004, Plant Physiol 136:2512-2522; Wang et al., 2003, Plant Physiol 132:556-567). In a related study, N-starved plants underwent a transcriptome/metabolome analysis 30 min and 3 h after nitrate treatment (Scheible, et al., 2004, Plant Physiol 136:2483-2499). The expression of nitrate transporters (at 30 min) preceded the induction of amino acid biosynthetic genes and the repression of amino acid breakdown genes (at 3 h). In addition, increases in amino acid levels were observed, consistent with the changes in expression of the cognate amino acid biosynthesis genes. Putative nitrate-responsive regulatory factors including transcription factors, protein kinases/phosphatases and trehalose and hormone metabolic genes were also identified in that study. Recently, using a NR-null mutant, it was shown that nitrate and not a product of nitrate reduction and assimilation, regulates the expression of genes involved in energy production, metabolism, glycolysis and gluconeogenesis (Wang et al., 2004, Plant Physiol 136:2512-2522).
Nitrogen metabolism genes can be regulated by negative feedback of the products of N assimilation including downstream organic N metabolites such as Glu or Gln. For example, the expression of the ammonium transporter gene ammonium transporter 1 (AMT1.1) is repressed in treatments with high levels of inorganic N. It has been shown that this repression is blocked by methionine sulfoximine (MSX), a non-metabolizable analog of Glu that irreversibly inhibits glutamine synthetase and hence blocks N assimilation into Gln (Rawat et al., 1999, Plant J 19:143-152). Thus, it appears that organic forms of N may regulate the uptake of N in plants. In addition, the genes encoding asparagine synthetase 1 (ASN1) and 2 (ASN2), are differentially regulated by organic and inorganic N sources. Organic N treatments were shown to positively regulate levels of ASN1 mRNA (Oliveira et al., 1999, Plant Physiol 121:301-310), whereas ASN2 gene expression appears to be responsive to inorganic N sources and not a downstream metabolite (Wang et al., 2004, Plant Physiol 136:2512-2522). Together, these studies prompt a model in which both inorganic as well as organic N sources can each regulate plant gene expression affecting N uptake, reduction and assimilation.
In study presented below, a genomic approach was used to identify gene networks whose expression is regulated by Glu or Glu-derived metabolites (organic N) in plants. Plants were treated with inorganic nitrogen sources in the presence or absence of MSX, which served to inhibit the assimilation of ammonium into Glu/Gln by blocking glutamine synthetase. The rationale for this approach was that a subset of nitrogen-responsive genes responding specifically to an organic signal (e.g. Glu/Gln) would not respond to nitrogen treatment if the synthesis of Glu/Gln was blocked by transient MSX treatments. Network analysis of the genes that responded to organic N revealed that transcription control of gene expression is important for a subnetwork of metabolic genes involved in the synthesis and degradation of asparagine (Asn), an important nitrogen-transport/storage compound synthesized when levels of nitrogen are abundant and degraded when nitrogen reserves are mobilized. The metabolic gene network discovered in this analysis provides molecular evidence for regulation of N-use at the level of gene expression. Moreover, the transcription factors regulated by organic N associated with this network provide a mechanistic link between circadian clock function and N-assimilation in plants.
7.2 Materials and Methods
Plant growth conditions. Wild type Arabidopsis thaliana ecotype Columbia-0 strain was used in all experiments unless indicated otherwise. Seeds were surface sterilized with ethanol and bleach as previously described (Brenner et al., 2000, Plant Physiol. 124:1615-1624) and sowed onto basal MS salts (Sigma, St Louis, Mo.) with 0.5% (w/v) sucrose, 0.8% BactoAgar, and 1 mM KNO3. After 14 days under long day (16 hours light: 8 hours dark) at 22° C., plants were transiently treated for 2 h in the light at the start of their light cycle by transferring them to basal medium with 0.5% sucrose and a combination of inorganic nitrogen sources (20 mM KNO3 and 20 mM NH4NO3) with or without 1 mM MSX (Sigma M-5379), 10 mM glutamate (Sigma G-1501) and/or 10 mM glutamine (Sigma G-3126): N; N+MSX; N+MSX+Glu respectively.
RNA isolation and quantitative real time PCR. RNA was isolated from whole plants with the TRIzol reagent and according to the instructions of the manufacturer (InVitrogen, Carlsbad, Calif.). cDNA synthesis from whole mRNA extractions was carried out according to kit manufacturer instructions (Invitrogen, Catalog number 11146-024). Real time quantitative PCR was carried out with a LightCycler (Roche Diagnostics, Mannheim, Germany) as described previously (Thum, K. E., Shasha, D. E., Lejay, L. V. & Coruzzi, G. M. (2003) Plant Physiol 132, 440-52).
Microarray experiments and analysis. cDNA synthesis, array hybridization, and normalization of the signal intensities were performed according to the instructions provided by Affymetrix (Santa Clara, Calif.). All raw microarray data was processed with MASv5.0 software as follows. Each hybridization was normalized to a median intensity of 150. Each treatment replica was compared with the two baselines to generate 4 comparisons per treatment. Data points with absent/marginal calls (Affymetrix quality control) in both baseline and treatment were removed. Data points with absent call in one hybridization and present call in the other hybridization were eliminated if the probe called present had a signal intensity of <100. The response of each gene was summarized using the Affymetrix change calls “I” for induced, “D” for decreased and “NC” for not changed. Data points were considered only if the change calls were consistent in at least 3 out of the 4 comparisons. This stepwise filtering resulted in a set of 834 genes that were detected and responded consistently in our experiments. We used custom made S-PLUS and PERL functions to analyze and visualize groups of genes with similar expression patterns based on the Affymetrix change calls.
Network analysis. For network analysis, an existing network model of plant gene interactions was used (Gutierrez, R. A., Lejay, L. V., Dean, A., Chiaromonte, F., Shasha, D. E. & Coruzzi, G. M. (2007) Genome Biol. 8(1):R7). In addition, protein:DNA interactions were predicted based as follows: The consensus sequence for transcription binding sites from well curated databases DATF (Guo, A., He, K., Liu, D., Bai, S., Gu, X., Wei, L. & Luo, J. (2005) Bioinformatics 21, 2568-2569) and AGRIS (Davuluri, R., Sun, H., Palaniswamy, S., Matthews, N., Molina, C., Kurtz, M. & Grotewold, E. (2003) BMC Bioinformatics 4, 25) were searched in 1500 base pairs of upstream sequence using the DNA pattern search tool from the RSA tools server with default parameters (van Helden, J. (2003) Nucleic Acids Res 31, 3593-3596). The search was performed in both strands of DNA, the upstream region was not allowed to overlap with the coding region of the upstream gene, motif matches were not allowed to overlap. A motif was considered over-represented if it was present in an upstream sequence more than 3 times the standard deviation above the mean occurrence in all the upstream sequences in the genome. A protein:DNA interaction was predicted when the upstream sequence of the gene contained an over representation of the regulatory motif for that transcription factor and the expression of the transcription factor and putative target gene was highly (≧0.7 or ≦−0.7) and significantly (p≦0.01) correlated. Similar regulatory predictions for other microarray data sets can be generated with the VirtualPlant system (http://www.virtualplant.org) using the “Gene Networks” tool.
Chromatin immunoprecipitation assays (ChIP): Immunoprecipitations (IP) were performed as previously described (Gendrel, A., Lippman, Z., Martienssen, R. A. & Colot, V. (2005) Profiling histone modification patterns in plants using genomic tiling microarrays Nat Methods 2, 219-224). Briefly, two weeks old wild-type and CCA1-ox plants were collected at the beginning of the light cycle and immediately fixed in 1% formaldehyde for 15 min in a vacuum at room temperature. Crosslinking was stopped by the addition of glycine to a final concentration of 0.125 M. Nuclei were prepared for chromatin isolation. The isolated chromatin was sonicated ten times for 20 s each at 100% power (Diagenode Bioruptor) in an ice water bath. A small aliquot of sheared chromatin was removed to serve as control. The diluted chromatin was used for IP with the CCA1 antibody and one control IP without antibody. The primer sequences used for amplification of the CCA1 binding sites in each of the genes tested are listed in Table 1.
| TABLE 1 | |||
| Gene | PUB locus | Primer 1 | Primer 2 |
| bZIP1 | At5g49450 | 5′-GATCGAAAATAAGGAAAGTGGG-3′ | 5′-ACTGGTCACCTATTAAGGAAC-3′ |
| (SEQ ID NO: 5) | (SEQ ID NO: 6) | ||
| TOC1 | At5g61380 | 5′-TGGACGGTGGAGATTAAGTC-3′ | 5′-ACGAAACGAAGCCGAATCCT-3′ |
| (SEQ ID NO: 7) | (SEQ ID NO: 8) | ||
| ZTL | At5g57360 | 5′-AGTCGCCGGAGATTATGAAGACGG- | 5′-GGTTTTATCTACTTGACCCGACAG-3′ |
| 3′ (SEQ ID NO: 9) | (SEQ ID NO: 10) | ||
| GDH1 | At5g18170 | 5′-TGTTTCAATAGCATTAGCCTCCA-3′ | 5′-TGGGGAATGTGACACACATAATC-3′ |
| (SEQ ID NO: 11) | (SEQ ID NO: 12) | ||
| GLN1.3 | At3g17820 | 5′-TTGAATCCGAAGAGGGGAAAA-3′ | 5′-AACAACTGCTACCAATTTCCTTG-3′ |
| (SEQ ID NO: 13) | (SEQ ID NO: 14) | ||
PCR amplifications included 95° C. for 2 min followed by 36 cycles of 95° C. for 15 s, 58° C. (for bZIP1, TOC1 and GDH1) or 60° C. (for ZTL and GLN1.3) for 30 s and 72° C. for 30 s.
Circadian phase response curves: CCA1::LUC seedlings were entrained on MS basal medium plus 0.5% sucrose and 1 mM KNO3 for 8 d in 16/8 h light/dark (100-150 μmol m−2 s−1), after which seedlings were moved into continuous light. At 3-hr intervals, seedlings (n=16 per treatment) were transferred to fresh solid medium plus 2 mL liquid Nms or medium containing 10 mM Glu or 10 mM Gln for 4 hr, then rinsed in liquid entrainment medium 3 times for a total of 30 min and transferred individually to the wells of 96-well microtiter plates containing fresh solid media for luciferase activity measurements which were determined with a Packard TopCount scintillation counter as described (Salome, P. A., et al. (2002) The out of phase 1 mutant defines a role for PHYB in circadian phase control in Arabidopsis Plant Physiol 129, 1674-85). The period and phase of rhythms after the pulses were determined by fast-Fourier transform nonlinear least-square analysis (Plautz, J. D., et al. (1997) Quantitative analysis of Drosophila period gene transcription in living animals J Biol Rhythms 12, 204-17). The phase shifts were calculated as described (Covington, M. F., et al. (2001) ELF3 modulates resetting of the circadian clock in Arabidopsis Plant Cell 13, 1305-15).
7.3 Results
Inorganic versus organic N responses. To uncouple gene responses to inorganic N from those elicited by downstream products of inorganic N assimilation, treatments of Arabidopsis seedlings with combinations of inorganic N (nitrate and ammonium), organic forms of N (e.g., Glu, Gln), and MSX, an inhibitor of glutamine synthetase were performed (King et al., 1993, Plant Pysiol. 102:1279-1286) (FIG. 7). Genes regulated by inorganic N signals should be unaffected by MSX treatment. By contrast, genes responding to a downstream organic N signal should fail to show induction by inorganic N treatments if Glu/Gln synthesis is blocked by MSX. This block of induction by MSX should be relieved by Glu treatment. Following this rationale, two-week-old Arabidopsis seedlings grown on low concentrations of N (1 mM NO3−) were transferred to media containing 40 mM NO3− and 20 mM NH4+ (referred to as “Nms”). Seedlings were then harvested after a 2 h treatment time. This treatment was carried out alone (Nms), in the presence of 1 mM MSX (Nms+MSX) or 1 mM MSX and 10 mM Glu (Nms+MSX+Glu). The Nms treatment consists of the same N source found in standard MS salts which is the established standard amount of N for plant growth (Murashige et al., 1962, Plant Physiol. 15:473-497). A concentration of 1 mM MSX has previously been established as effective in blocking the N repression of AMT1.1 in Arabidopsis seedlings and in decreasing levels of internal organic N (Rawat et al., 1999, Plant J 19:143-152). A concentration of 10 mM for Glu treatments was chosen because this has been shown to be effective in the regulation of N assimilatory genes while not being high enough to be detrimental to plant growth or development (Oliveria et al., 1999, Plant Physiol. 121-301-310). To evaluate the effect of MSX alone, plants were exposed to growth media that contained MSX. To control for the effect of the plant transfer to distinct media, plants were transferred onto media plates without any of the treatment factors. This latter control was used as the base line for the microarray experiments described below.
To evaluate the experimental design, the mRNA level of genes shown to be responsive to organic N (ASN1) or inorganic N (ASN2) was determined by reverse transcription followed by real time quantitative PCR(RT-qPCR). This analysis showed that the ASN1 mRNA level was induced 3.5-fold by the Nms treatment as compared to the control (FIG. 8, compare Nms versus the control). This induction of ASN1 mRNA was blocked when MSX was present (FIG. 8A, Nms+MSX versus Nms). Importantly, when exogenous Glu or Gln was added, ASN1 levels were induced regardless of the presence of MSX (FIG. 8A, compare Control to Nms+MSX+Glu and Nms+MSX+Gln). These results indicate that the induction of ASN1 is due to Glu or a downstream metabolite, as shown previously (Lam et al., 1998, Plant J 16:345-353; Oliveira et al., 2001, Braz J Med Biol Res 34:567-575). In addition, the control treatments showed that MSX alone does not induce expression of ASN1 or ASN2 (FIG. 8). The addition of Glu or Gln partially blocked the induction of ASN2 by the Nms treatment (FIG. 8B), consistent with the negative regulation by amino acids seen previously (Lam et al., 1998, Plant J 16:345-353). Conversely, the induction of ASN2 mRNA by Nms was insensitive to MSX addition (FIG. 8B), suggesting the induction was mediated by an inorganic N source. This finding was consistent with previous data which indicates that ASN2 gene expression correlates with ammonium levels (Wong et al., 2004, Plant Physiol 134:332-812).
Global genomic responses to organic and inorganic nitrogen signals. To investigate global gene expression changes that are mediated by Glu or a Glu-derived metabolite, the plant transcriptome was monitored using the ATH1 Affymetrix gene chip. Total RNA was extracted from plants treated with Nms, Nms+MSX or Nms+MSX+Glu as described above; two biological replicates per treatment were performed. The Nms+MSX+Gln treatments were not analyzed using microarrays because Gln and Glu responses were similar in our hands (FIG. 8). RNA was labeled and hybridized to the microarrays, the raw intensity values were normalized and the data filtered as described in Materials and Methods. A gene was kept in the data set only if its expression was reproducible and reliable across the 3 different treatments (Nms, Nms+MSX, Nms+MSX+Glu). A total of 5,904 genes were identified that passed these stringent quality control criteria. In order to verify the microarray results, we analyzed the mRNA levels of selected genes by RT-qPCR including the TAZ zinc binding (At4g37610) and bZIP (At5g49450) transcription factors with results similar to the microarray data (FIG. 9). As expected, the genomic experiments verified the previous observation that AMT1.1 and ASN1 are regulated by organic N. In addition, the results identified additional genes regulated by organic N as described below.
Genes were categorized based on their response to the treatments using the Affymetrix change calls: induced (I), no change (NC) or decreased (D). Each gene was assigned a three-part code (e.g. I-NC-I) which corresponds to the gene expression response in the Nms, Nms+MSX, and Nms+MSX+Glu treatments respectively. 21 unique patterns of response were found (See Annex) and Table 2 (in each column the gene listed on the left is predicted to control the expression of the gene on the right, i.e., At1g74840 is predicted to control the expression of At2g47060, At5g24800 is predicted to control the expression of At5g13930).
| TABLE 2 | |||||
| At1g74840 | reg0.9 | At2g47060 | At5g24800 | reg0.7 | At5g13930 |
| At5g14540 | reg0.9 | At5g53370 | At5g48655 | reg0.8 | At5g53370 |
| At3g61150 | reg0.7 | At4g30810 | At1g74840 | reg0.8 | At5g01820 |
| At2g46830 | reg0.8 | At5g01820 | At5g24800 | reg0.8 | At2g30040 |
| At5g48655 | reg0.7 | At2g30040 | At2g33710 | reg0.8 | At2g30040 |
| At4g17490 | reg0.8 | At2g30040 | At2g46830 | reg0.7 | At2g30040 |
| At5g44190 | reg0.8 | At2g30040 | At1g74840 | reg0.7 | At4g28100 |
| At2g20570 | reg0.8 | At4g28100 | At1g22070 | reg0.7 | At4g33300 |
| At2g46830 | reg0.7 | At1g06000 | At5g48655 | reg0.9 | At5g11790 |
| At1g74840 | reg0.8 | At4g36640 | At1g22070 | reg0.8 | At4g19810 |
| At5g24800 | reg0.9 | At4g19810 | At5g49450 | reg0.9 | At2g39980 |
| At3g01560 | reg0.7 | At2g15970 | At1g43160 | reg0.9 | At2g15970 |
| At1g22070 | reg0.7 | At2g15970 | At2g20570 | reg0.7 | At2g22240 |
| At5g49450 | reg0.8 | At1g49500 | At2g20570 | reg0.8 | At1g27730 |
| At2g46830 | reg0.9 | At1g27730 | At4g37260 | reg0.7 | At3g04070 |
| At5g24800 | reg0.8 | At2g36290 | At5g48655 | reg0.7 | At5g63790 |
| At5g48655 | reg0.7 | At4g28250 | At2g04880 | reg0.9 | At5g45340 |
| At2g25000 | reg0.7 | At5g45340 | At2g38470 | reg1.0 | At5g45340 |
| At5g44190 | reg0.8 | At1g29670 | At5g14540 | reg0.7 | At1g70330 |
| At1g53910 | reg0.7 | At1g70330 | At5g14540 | reg0.8 | At3g58560 |
| At2g22430 | reg0.8 | At2g30870 | At5g47230 | reg0.7 | At2g30870 |
| At5g61890 | reg0.7 | At2g30870 | At2g22430 | reg0.8 | At2g35930 |
| At2g38470 | reg0.7 | At1g33590 | At3g01560 | reg0.7 | At5g06320 |
| At5g14540 | reg0.7 | At5g06320 | At3g61150 | reg0.7 | At2g44210 |
| At5g49450 | reg1.0 | At2g44080 | At3g61890 | reg0.7 | At5g60850 |
| At1g22070 | reg0.7 | At3g59220 | At2g20570 | reg0.7 | At1g60780 |
| At1g43160 | reg0.7 | At1g74840 | At5g61890 | reg0.8 | At1g74840 |
| At5g49450 | reg0.9 | At4g27410 | At5g14540 | reg0.8 | At2g40270 |
| At4g37260 | reg0.7 | At1g77510 | At5g14540 | reg0.8 | At4g33400 |
| At1g74840 | reg0.7 | At4g36250 | At3g61890 | reg0.9 | At5g05600 |
| At1g74840 | reg0.9 | At1g53310 | At2g22430 | reg0.7 | At4g14960 |
| At1g43160 | reg0.9 | At5g15960 | At5g61890 | reg0.9 | At5g15960 |
| At2g22430 | reg0.8 | At3g23750 | At1g53910 | reg0.8 | At3g23750 |
| At5g61890 | reg0.8 | At3g19680 | At3g01560 | reg0.8 | At2g47180 |
| At1g43160 | reg0.7 | At2g47180 | At5g47230 | reg0.8 | At2g47180 |
| At5g61890 | reg0.7 | At2g47180 | At2g22430 | reg0.7 | At5g01540 |
| At5g14540 | reg0.7 | At2g39530 | At5g48655 | reg0.8 | At2g39530 |
| At2g33710 | reg0.8 | At2g39530 | At1g74840 | reg0.9 | At1g51680 |
| At1g22070 | reg0.8 | At4g39330 | At5g24800 | reg0.7 | At4g39330 |
| At1g53910 | reg0.9 | At4g03260 | At5g14540 | reg0.8 | At1g76670 |
| At2g22430 | reg0.7 | At1g76670 | At3g61150 | reg0.7 | At1g76670 |
| At5g24800 | reg0.9 | At5g02270 | At2g20570 | reg0.8 | At5g35735 |
| At5g61890 | reg0.8 | At3g47960 | At1g74840 | reg0.8 | At3g21230 |
| At2g04880 | reg0.8 | At4g31500 | At4g31800 | reg0.7 | At4g31500 |
| At3g61150 | reg0.8 | At5g63850 | At3g01560 | reg0.7 | At2g46600 |
| At4g37260 | reg0.9 | At1g10760 | At1g74840 | reg0.8 | At3g54640 |
| At5g49450 | reg0.7 | At1g76590 | At1g25560 | reg0.7 | At1g76590 |
| At1g68840 | reg0.8 | At1g76590 | At5g24800 | reg0.7 | At5g44190 |
| At1g74840 | reg0.9 | At5g60920 | At5g14540 | reg0.8 | At5g60920 |
| At1g25560 | reg0.8 | At4g38470 | At1g68840 | reg0.7 | At4g38470 |
| At2g20570 | reg0.8 | At2g37430 | At2g46830 | reg0.9 | At2g37430 |
| At2g04880 | reg0.8 | At2g37430 | At2g25000 | reg0.8 | At2g37430 |
| At2g30250 | reg0.7 | At2g37430 | At2g38470 | reg0.9 | At2g37430 |
| At4g01250 | reg0.7 | At2g37430 | At2g20570 | reg0.9 | At1g74460 |
| At2g46830 | reg0.8 | At1g74460 | At1g22070 | reg0.7 | At4g23630 |
| At3g01560 | reg0.9 | At3g14280 | At5g14540 | reg0.7 | At3g14280 |
| At3g61150 | reg0.7 | At3g14280 | At2g20570 | reg0.7 | At3g14280 |
| At2g46830 | reg0.8 | At3g14280 | At2g04880 | reg0.8 | At5g44070 |
| At2g38470 | reg0.9 | At5g44070 | At4g01250 | reg0.7 | At5g44070 |
| At5g24800 | reg0.7 | At1g73080 | At2g33710 | reg0.9 | At3g56710 |
| At4g17490 | reg0.9 | At3g56710 | At2g20570 | reg0.8 | At3g56710 |
| At2g46830 | reg0.8 | At3g56710 | At1g74840 | reg0.8 | At2g30490 |
| At3g01560 | reg0.7 | At2g42540 | At1g43160 | reg0.9 | At2g42540 |
| At5g61890 | reg0.8 | At2g42540 | At1g74840 | reg0.7 | At1g47128 |
| At2g46830 | reg0.7 | At1g47128 | At5g24800 | reg0.7 | At4g11280 |
| At2g20570 | reg0.7 | At3g60030 | At4g37260 | reg0.7 | At3g47620 |
| At5g49450 | reg0.8 | At3g21870 | At5g14540 | reg0.8 | At4g33050 |
| At5g48655 | reg0.7 | At4g33050 | At3g01560 | reg0.7 | At5g01600 |
| At1g43160 | reg0.8 | At5g01600 | At1g43160 | reg0.8 | At3g05890 |
| At1g74840 | reg0.7 | At2g16630 | At4g37260 | reg0.9 | At2g16630 |
| At2g04880 | reg0.9 | At5g45340 | At2g25000 | reg0.7 | At5g45340 |
| At2g38470 | reg1.0 | At5g45340 | At3g01560 | reg0.7 | At2g22880 |
| At5g48655 | reg0.7 | At2g22880 | At2g04880 | reg0.8 | At3g52400 |
| At1g43160 | reg0.9 | At1g29395 | At5g61890 | reg0.7 | At1g29395 |
| At1g74840 | reg0.8 | At2g16430 | At1g74840 | reg0.8 | At4g23210 |
| At1g22070 | reg0.7 | At4g23210 | At5g49450 | reg0.8 | At4g21150 |
| At1g22070 | reg0.9 | At3g48610 | At5g24800 | reg0.7 | At3g48610 |
| At5g47230 | reg0.7 | At2g38700 | At1g74840 | reg0.7 | At4g30280 |
| At5g14540 | reg0.7 | At1g53500 | At5g48655 | reg0.8 | At1g53500 |
| At3g01560 | reg0.7 | At2g23120 | At5g14540 | reg0.8 | At5g09440 |
| At2g22430 | reg0.8 | At5g09440 | At1g74840 | reg0.8 | At5g09440 |
| At5g24800 | reg0.7 | At2g41630 | At2g20570 | reg0.8 | At1g56150 |
| At2g46830 | reg0.8 | At1g56150 | At5g49450 | reg0.7 | At1g10070 |
| At2g22430 | reg0.8 | At3g21240 | At3g01560 | reg0.8 | At5g16010 |
| At4g37260 | reg0.9 | At5g46710 | At1g25560 | reg0.7 | At5g11420 |
| At1g68840 | reg0.7 | At5g11420 | At5g24800 | reg0.7 | At4g01250 |
| At5g44190 | reg0.7 | At3g07790 | At2g30250 | reg0.7 | At1g13110 |
| At2g38470 | reg0.8 | At1g13110 | At4g01250 | reg0.9 | At1g13110 |
| At1g22070 | reg0.8 | At2g25000 | At2g20570 | reg0.9 | At2g25000 |
| At2g46830 | reg0.8 | At2g25000 | At2g04880 | reg0.8 | At2g25000 |
| At2g38470 | reg0.7 | At2g25000 | At4g31800 | reg0.8 | At2g25000 |
| At5g24800 | reg0.7 | At1g35780 | At3g01560 | reg0.8 | At5g11110 |
| At1g74840 | reg0.9 | At5g11110 | At1g43160 | reg0.8 | At5g11110 |
| At5g47230 | reg0.7 | At5g11110 | At5g61890 | reg0.7 | At5g11110 |
| At3g61890 | reg0.8 | At4g12490 | At1g74840 | reg0.7 | At1g51700 |
| At1g74840 | reg0.8 | At3g55970 | At4g37260 | reg0.7 | At3g55970 |
| At4g17500 | reg0.8 | At5g49910 | At1g22070 | reg0.7 | At4g39800 |
| At1g74840 | reg0.8 | At4g39800 | At2g20570 | reg0.7 | At4g39800 |
| At2g46830 | reg0.8 | At4g39800 | At2g20570 | reg0.8 | At2g13790 |
| At2g46830 | reg0.8 | At2g13790 | At3g61890 | reg1.0 | At1g52400 |
| At2g20570 | reg0.8 | At5g18470 | At2g46830 | reg0.7 | At5g18470 |
| At2g22430 | reg0.9 | At1g53910 | At5g14540 | reg0.9 | At2g40140 |
| At5g48655 | reg0.9 | At2g40140 | At2g20570 | reg0.8 | At2g40890 |
| At2g46830 | reg0.8 | At2g40890 | At1g74840 | reg0.7 | At3g55070 |
| At4g37260 | reg0.7 | At1g68520 | At1g74840 | reg0.9 | At1g14780 |
| At1g43160 | reg0.8 | At1g14780 | At5g61890 | reg0.9 | At1g14780 |
| At2g22430 | reg0.9 | At2g39210 | At2g20570 | reg0.9 | At1g76600 |
| At2g46830 | reg0.9 | At1g76600 | At2g04880 | reg0.9 | At1g76600 |
| At2g25000 | reg0.8 | At1g76600 | At2g38470 | reg0.8 | At1g76600 |
| At5g48655 | reg0.8 | At5g19240 | At2g22430 | reg0.7 | At3g02910 |
| At2g22430 | reg0.9 | At3g63010 | At3g61890 | reg0.9 | At3g49120 |
| At1g74840 | reg0.7 | At3g49120 | At4g37260 | reg0.8 | At3g49120 |
| At5g14540 | reg0.7 | At3g52450 | At2g23320 | reg0.7 | At3g52450 |
| At4g31800 | reg0.9 | At3g52450 | At1g25560 | reg0.7 | At5g58710 |
| At1g68840 | reg0.7 | At5g58710 | At5g49450 | reg0.7 | At5g58710 |
| At3g01560 | reg0.8 | At4g25650 | At1g53910 | reg0.7 | At4g04020 |
| At4g17490 | reg0.7 | At4g04020 | At5g49450 | reg0.9 | At3g47340 |
| At5g49450 | reg0.8 | At1g77120 | At5g48655 | reg0.7 | At2g26190 |
| At4g37260 | reg0.7 | At5g49450 | At1g22070 | reg0.9 | At1g20440 |
| At1g22070 | reg0.7 | At5g59820 | At5g48655 | reg0.8 | At1g26250 |
| At1g74840 | reg0.8 | At5g07010 | At4g37260 | reg0.7 | At5g07010 |
| At1g74840 | reg0.7 | At3g54690 | At2g20570 | reg0.7 | At3g54690 |
| At2g46830 | reg0.8 | At3g54690 | At2g20570 | reg0.8 | At5g26920 |
| At2g46830 | reg0.7 | At5g26920 | At3g61890 | reg0.8 | At2g02990 |
| At1g74840 | reg0.8 | At5g61890 | At5g49450 | reg0.7 | At2g34500 |
| At1g74840 | reg0.8 | At4g01700 | At2g46830 | reg0.7 | At4g01700 |
| At2g20570 | reg0.9 | At5g59730 | At2g46830 | reg0.9 | At5g59730 |
| At3g01560 | reg0.8 | At3g52470 | At5g14540 | reg0.8 | At3g52470 |
| At5g48655 | reg0.7 | At3g52470 | At2g46830 | reg0.8 | At3g02800 |
| At5g49450 | reg0.8 | At4g24800 | At4g37260 | reg0.7 | At4g24800 |
| At2g20570 | reg0.8 | At5g25630 | At2g46830 | reg0.7 | At5g25630 |
| At5g24800 | reg0.7 | At5g28900 | At2g20570 | reg0.7 | At4g27280 |
| At2g46830 | reg0.8 | At4g27280 | At5g49450 | reg0.9 | At3g13450 |
| At5g24800 | reg0.9 | At1g20510 | At2g20570 | reg0.7 | At1g20510 |
| At2g46830 | reg0.8 | At1g20510 | At5g44190 | reg0.7 | At1g20510 |
| At2g46830 | reg0.7 | At1g14730 | At5g61890 | reg0.7 | At1g43160 |
| At3g01560 | reg0.8 | At5g06700 | At5g14540 | reg0.8 | At5g06700 |
| At1g22070 | reg0.7 | At5g06700 | 1g74840 | reg0.8 | At5g06700 |
| At2g20570 | reg0.7 | At5g06700 | 5g24800 | reg0.7 | At4g34450 |
| At1g43160 | reg1.0 | At2g28900 | At1g74840 | reg0.7 | At2g28900 |
| At4g37260 | reg0.7 | At2g28900 | 2g20570 | reg0.7 | At5g42310 |
| At3g01560 | reg0.8 | At5g42310 | At5g14540 | reg0.8 | At5g42310 |
| At3g01560 | reg0.7 | At2g43620 | At3g61890 | reg0.8 | At2g43620 |
| At1g74840 | reg0.7 | At3g23810 | At2g20570 | reg0.8 | At3g17820 |
| At2g46830 | reg0.7 | At3g17820 | At3g01560 | reg0.8 | At3g23820 |
| At5g14540 | reg0.7 | At3g23820 | At1g74840 | reg0.8 | At3g23820 |
| At1g43160 | reg0.7 | At3g23820 | At5g47230 | reg0.8 | At3g23820 |
| At5g61890 | reg0.8 | At3g23820 | At5g49450 | reg0.9 | At3g57520 |
| At3g61890 | reg0.9 | At4g22212 | At2g30250 | reg0.7 | At2g46225 |
| At4g01250 | reg0.7 | At2g46225 | At4g23810 | reg0.8 | At2g46225 |
| At5g49450 | reg0.7 | At4g37260 | At5g24800 | reg0.7 | At2g24940 |
| At1g25560 | reg0.9 | At3g19390 | At1g68840 | reg0.9 | At3g19390 |
| At3g01560 | reg0.8 | At1g21790 | At1g74840 | reg0.8 | At1g21790 |
| At4g37260 | reg0.8 | At1g21790 | At4g37260 | reg0.7 | At1g12780 |
| At2g20570 | reg0.7 | At5g54490 | At2g46830 | reg0.9 | At5g54490 |
| At1g22070 | reg0.9 | At3g11670 | At5g24800 | reg0.7 | At3g11670 |
| At5g49450 | reg-0.7 | At2g30040 | At1g25560 | reg-0.7 | At2g30040 |
| At1g68840 | reg-0.7 | At2g30040 | At2g20570 | reg-0.9 | At1g80180 |
| At2g46830 | reg-0.8 | At1g80180 | At1g22070 | reg-0.7 | At1g06760 |
| At5g24800 | reg-0.8 | At5g61790 | At5g48655 | reg-0.7 | At1g11545 |
| At2g20570 | reg-0.8 | At1g07040 | At2g46830 | reg-0.8 | At1g07040 |
| At4g17500 | reg-0.7 | At2g15970 | At2g22430 | reg-0.9 | At1g49500 |
| At5g44190 | reg-0.7 | At3g04070 | At4g37260 | reg-0.8 | At4g22710 |
| At5g49450 | reg-0.8 | At5g63790 | At5g14540 | reg-0.7 | At3g60320 |
| At5g48655 | reg-0.9 | At3g60320 | At2g22430 | reg-0.7 | At3g60320 |
| At3g01560 | reg-0.7 | At4g37450 | At4g37260 | reg-0.7 | At1g77450 |
| At1g22070 | reg-0.7 | At1g42480 | At2g46830 | reg-0.7 | At5g64570 |
| At2g20570 | reg-0.8 | At5g39610 | At2g22430 | reg-0.8 | At5g39610 |
| At3g61890 | reg-0.7 | At2g34640 | At5g24800 | reg-0.8 | At4g16660 |
| At2g20570 | reg-0.7 | At5g60680 | At2g46830 | reg-0.7 | At5g60680 |
| At4g17500 | reg-0.7 | At2g47180 | At1g22070 | reg-0.9 | At1g09210 |
| At2g04880 | reg-0.7 | At1g68840 | At2g38470 | reg-0.8 | At1g68840 |
| At2g22430 | reg-0.7 | At1g49860 | At5g44190 | reg-0.8 | At1g09240 |
| At1g74840 | reg-0.7 | At1g76690 | At4g37260 | reg-0.9 | At1g76690 |
| At1g43160 | reg-0.9 | At4g31130 | At5g61890 | reg-0.7 | At4g31130 |
| At1g74840 | reg-0.7 | At4g31130 | At4g37260 | reg-0.8 | At4g31130 |
| At5g49450 | reg-0.9 | At2g38470 | At5g44190 | reg-0.7 | At4g39980 |
| At5g14540 | reg-0.9 | At4g12600 | At4g37260 | reg-0.8 | At3g25230 |
| At1g74840 | reg-0.8 | At1g32170 | At2g20570 | reg-0.7 | At1g32170 |
| At2g46830 | reg-0.7 | At1g32170 | At1g43160 | reg-0.7 | At2g29490 |
| At5g47230 | reg-0.7 | At2g29490 | At5g61890 | reg-0.8 | At2g29490 |
| At5g14540 | reg-0.8 | At5g05440 | At5g48655 | reg-0.8 | At5g05440 |
| At2g33710 | reg-0.8 | At4g39675 | At2g20570 | reg-0.7 | At2g04280 |
| At2g46830 | reg-0.7 | At2g04280 | At1g74840 | reg-0.7 | At5g49480 |
| At2g22430 | reg-0.7 | At5g49480 | At3g61150 | reg-0.7 | At5g49480 |
| At5g48655 | reg-0.7 | At1g76590 | At4g37260 | reg-0.7 | At1g32920 |
| At2g33710 | reg-0.9 | At4g38470 | At4g17490 | reg-0.8 | At4g38470 |
| At2g20570 | reg-0.7 | At4g38470 | At2g46830 | reg-0.8 | At4g38470 |
| At5g44190 | reg-0.7 | At5g15410 | At2g20570 | reg-0.7 | At1g67910 |
| At2g46830 | reg-0.8 | At1g67910 | At3g01560 | reg-0.7 | At5g25460 |
| At5g14540 | reg-0.9 | At5g25460 | At5g48655 | reg-0.8 | At5g25460 |
| At3g61150 | reg-0.7 | At3g48990 | At1g22070 | reg-0.8 | At3g48990 |
| At5g24800 | reg-0.8 | At3g48990 | At5g49450 | reg-0.7 | At3g62960 |
| At1g25560 | reg-0.8 | At3g56710 | At1g68840 | reg-0.7 | At3g56710 |
| At5g44190 | reg-0.7 | At3g51550 | At1g74840 | reg-0.8 | At3g62120 |
| At4g17500 | reg-0.7 | At2g42540 | At5g49450 | reg-0.8 | At4g11280 |
| At1g22070 | reg-0.8 | At3g15950 | At3g01560 | reg-0.9 | At3g19130 |
| At1g74840 | reg-0.7 | At4g21620 | At4g37260 | reg-0.8 | At4g21620 |
| At5g14540 | reg-0.7 | At3g21870 | At5g48655 | reg-0.7 | At3g21870 |
| At2g20570 | reg-0.8 | At3g15450 | At2g46830 | reg-0.8 | At3g15450 |
| At4g17500 | reg-0.7 | At5g01600 | At4g17500 | reg-0.7 | At3g05890 |
| At2g46830 | reg-0.8 | At1g22570 | At5g48655 | reg-0.9 | At3g14310 |
| At3g61150 | reg-0.8 | At5g65390 | At2g20570 | reg-0.8 | At1g76160 |
| At2g46830 | reg-0.7 | At1g76160 | At4g17500 | reg-0.7 | At1g29395 |
| At5g49450 | reg-0.7 | At5g66510 | At1g74840 | reg-0.7 | At1g62480 |
| At5g44190 | reg-0.8 | At3g07390 | At2g20570 | reg-0.8 | At2g33830 |
| At2g46830 | reg-0.8 | At2g33830 | At3g61890 | reg-0.7 | At3g06680 |
| At4g37260 | reg-0.8 | At5g23020 | At5g24800 | reg-0.7 | At1g10070 |
| At5g48655 | reg-0.8 | At4g36670 | At2g38470 | reg-0.9 | At4g36670 |
| At1g22070 | reg-0.7 | At2g44310 | At5g24800 | reg-0.7 | At2g44310 |
| At2g22430 | reg-0.7 | At2g44310 | At3g61150 | reg-0.7 | At2g44310 |
| At3g01560 | reg-0.7 | At4g12880 | At5g14540 | reg-0.8 | At4g12880 |
| At5g48655 | reg-0.9 | At4g12880 | At1g74840 | reg-0.8 | At4g13770 |
| At4g37260 | reg-0.7 | At4g13770 | At3g61150 | reg-0.7 | At3g54400 |
| At5g44190 | reg-0.7 | At5g06530 | At5g48655 | reg-0.9 | At5g11420 |
| At2g33710 | reg-0.9 | At5g11420 | At4g17490 | reg-0.8 | At5g11420 |
| At1g74840 | reg-0.7 | At1g19870 | At4g37260 | reg-0.7 | At1g19870 |
| At1g22070 | reg-0.8 | At1g19870 | At4g17500 | reg-0.7 | At1g35780 |
| At4g17500 | reg-0.7 | At5g11110 | At5g49450 | reg-0.9 | At1g10960 |
| At5g44190 | reg-0.7 | At5g06530 | At5g44190 | reg-0.7 | At3g55970 |
| At4g37260 | reg-0.8 | At3g11700 | At3g01560 | reg-0.7 | At5g49910 |
| At3g61890 | reg-0.8 | At4g23180 | At1g74840 | reg-0.9 | At4g23180 |
| At4g37260 | reg-0.7 | At4g23180 | At1g74840 | reg-0.8 | At4g10480 |
| At2g46830 | reg-0.7 | At4g10480 | At2g22430 | reg-0.7 | At5g43970 |
| At3g61150 | reg-0.7 | At5g43970 | At3g61890 | reg-0.7 | At5g11670 |
| At1g22070 | reg-0.7 | At1g56330 | At5g48655 | reg-0.7 | At1g68520 |
| At5g44190 | reg-0.7 | At1g68520 | At3g61150 | reg-0.7 | At1g31420 |
| At5g44190 | reg-1.0 | At2g23810 | At2g20570 | reg-0.9 | At3g15630 |
| At2g46830 | reg-0.8 | At3g15630 | At2g20570 | reg-0.9 | At2g39570 |
| At2g46830 | reg-0.8 | At2g39570 | At5g49450 | reg-0.8 | At1g76600 |
| At1g74840 | reg-0.9 | At2g06850 | At5g14540 | reg-0.7 | At2g37640 |
| At5g48655 | reg-0.7 | At2g37640 | At2g20570 | reg-0.8 | At2g37640 |
| At2g46830 | reg-0.9 | At2g37640 | At1g74840 | reg-0.7 | At1g02930 |
| At4g37260 | reg-0.8 | At1g02930 | At2g33710 | reg-0.8 | At5g58710 |
| At3g61890 | reg-0.7 | At1g64640 | At1g74840 | reg-0.8 | At1g64640 |
| At2g20570 | reg-0.8 | At3g49780 | At2g46830 | reg-0.9 | At3g49780 |
| At5g24800 | reg-0.8 | At3g49780 | At5g24800 | reg-0.7 | At3g47340 |
| At5g44190 | reg-0.8 | At1g09330 | At5g48655 | reg-0.7 | At5g49450 |
| At2g20570 | reg-0.8 | At5g49450 | At2g46830 | reg-0.7 | At5g49450 |
| At5g44190 | reg-0.8 | At5g07010 | At5g49450 | reg-0.7 | At2g29550 |
| At1g22070 | reg-0.7 | At2g34500 | At1g74840 | reg-0.8 | At3g61440 |
| At5g14540 | reg-0.8 | At2g24500 | At5g24800 | reg-0.7 | At5g11520 |
| At4g37260 | reg-0.7 | At1g62380 | At5g48655 | reg-0.7 | At1g80070 |
| At2g22430 | reg-0.8 | At3g13450 | At5g14540 | reg-0.8 | At1g24530 |
| At1g22070 | reg-0.7 | At1g24530 | At5g49450 | reg-0.7 | At1g20510 |
| At5g14540 | reg-0.7 | At1g73120 | At4g17500 | reg-0.7 | At1g43160 |
| At1g74840 | reg-0.9 | At1g53240 | At2g20570 | reg-0.8 | At5g18170 |
| At2g46830 | reg-0.8 | At5g18170 | At3g61890 | reg-0.8 | At2g30010 |
| At1g74840 | reg-0.7 | At2g30010 | At4g37260 | reg-0.9 | At2g30010 |
| At5g49450 | reg-0.7 | At2g30010 | At5g24800 | reg-0.9 | At5g49700 |
| At4g17500 | reg-0.8 | At2g28900 | At5g24800 | reg-0.8 | At1g15690 |
| At5g44190 | reg-0.8 | At4g12480 | At2g04880 | reg-0.9 | At5g22920 |
| At2g25000 | reg-0.7 | At5g22920 | At2g38470 | reg-1.0 | At5g22920 |
| At5g44190 | reg-0.8 | At3g23810 | At5g48655 | reg-0.8 | At3g57520 |
| At2g20570 | reg-0.7 | At3g57520 | At2g46830 | reg-0.8 | At3g57520 |
| At5g24800 | reg-0.7 | At3g57520 | At1g53910 | reg-0.8 | At5g04340 |
| At5g24800 | reg-0.8 | At2g05380 | At2g33710 | reg-0.7 | At3g19390 |
| At4g17490 | reg-0.8 | At3g19390 | At4g37260 | reg-0.8 | At3g12740 |
| At2g20570 | reg-0.7 | At1g12780 | At3g61890 | reg-0.8 | At3g16530 |
The largest pattern was NC-NC-NC, representing 5,070 genes not affected by the treatments. The remainder 20 patterns (834 genes) were grouped into six classes that summarize the types of N-responses observed: (A) inorganic N, (B) inorganic N with Glu feedback, (C) organic N with no exogenous Glu rescue, (D) exogenous Glu, (E) exogenous and endogenous Glu and (F) exogenous and endogenous Glu with opposite effects (Table 3).
| TABLE 3 | ||||||
| Treatment | ||||||
| Nms + | Genes | |||||
| Nms + | MSX + | per | ||||
| Response | Nms | MSX | Glu | Genes | class | |
| A) | Inorganic nitrogen | D | D | D | 100 | 159 |
| I | I | I | 59 | |||
| B) | Inorganic nitrogen | D | D | NC | 30 | 48 |
| and Glu | I | I | NC | 15 | ||
| D | D | I | 3 | |||
| C) | Internal Glu | D | NC | NC | 194 | 334 |
| I | NC | NC | 56 | |||
| NC | I | I | 49 | |||
| NC | D | D | 33 | |||
| D | I | I | 2 | |||
| D) | External Glu | NC | NC | D | 89 | 126 |
| NC | NC | I | 37 | |||
| E) | Internal/External Glu | NC | I | NC | 55 | 164 |
| NC | D | NC | 36 | |||
| D | NC | D | 47 | |||
| I | NC | I | 22 | |||
| I | D | I | 3 | |||
| NC | D | I | 1 | |||
| F) | Opposite | D | NC | I | 2 | 3 |
| Internal/External Glu | I | NC | D | 1 | ||
| 834 | ||||||
| Nitrogen responses. | ||||||
| Patterns of expression based on their response to the Nms, Nms + MSX and Nms + MSX + Glu treatments. | ||||||
| D = decreased; | ||||||
| I = increased; | ||||||
| NC = not changed. | ||||||
| Genes were categorized into six classes based on these patterns. | ||||||
The genes regulated in the experiments were compared to published results (Wang et al., 2004, Plant Physiol 136:2512-2522). This previous study identified 595 genes that responded similarly to nitrate treatment in both a NR-null mutant and wild-type plants. Because the mutant plants cannot assimilate nitrate, the responses observed were attributed to the action of nitrate as a signal and not a downstream metabolite. 80 out of these 595 genes showed consistent and reliable responses in the current experiments. Surprisingly, only 17 of these genes were found regulated by inorganic N signals in both studies (Table 4). Among these, we found nitrite reductase and several high affinity nitrate transporters. The majority, 58 of these 80 genes (73%), belonged to the C, D or E classes in the present studies suggesting that many previously described nitrate-responsive genes may respond to organic N signals (Table 4).
| TABLE 4 | ||
| Regulated by | PUB LOCUS | Gene Name |
| Inorganic N | At4g19170 | 9-cis-epoxycarotenoid dioxygenase, putative/neoxanthin cleavage enzyme, |
| putative/carotenoid cleavage dioxygenase, putative similar to 9-cis- | ||
| epoxycarotenoid dioxygenase [Phaseolus vulgaris][GI: 6715257]; neoxanthin | ||
| cleavage enzyme, Lycopersicon esculentum, PATX: E325797 | ||
| (68417.m02829) | ||
| Inorganic N | At3g61820 | aspartyl protease family protein contains Pfam domain, PF00026: eukaryotic |
| aspartyl protease (68416.m06939) | ||
| Inorganic N | At4g30190 | ATPase 2, plasma membrane-type, putative/proton pump 2, putative/proton- |
| exporting ATPase, putative strong similarity to SP | ||
| Inorganic N | At4g31500 | cytochrome P450 83B1 (CYP83B1) Identical to Cytochrome P450 |
| (SP: O65782)[Arabidopsis thaliana] (68417.m04474) | ||
| Inorganic N | At1g05340 | expressed protein (68414.m00541) |
| Inorganic N | At1g19020 | expressed protein (68414.m02367) |
| Inorganic N | At1g32920 | expressed protein (68414.m04055) |
| Inorganic N | At2g41730 | expressed protein (68415.m05158) |
| Inorganic N | At1g14870 | expressed protein similar to PGPS/D12 [Petunia x hybrida] GI: 4105794; |
| contains Pfam profile PF04749: Protein of unknown function, DUF614 | ||
| (68414.m01778) | ||
| Inorganic N | At5g25350 | F-box family protein contains Pfam PF00646: F-box domain and Pfam PF00560: |
| Leucine Rich Repeat (6 copies); similar to F-box protein | ||
| FBL6 (GI: 4432860) [Homo sapiens] (68418.m03007) | ||
| Inorganic N | At2g15620 | ferredoxin--nitrite reductase, putative strong similarity to ferredoxin--nitrite |
| reductase [Nicotiana tabacum] GI: 19893; contains Pfam profiles | ||
| PF03460: Nitrite/Sulfite reductase ferredoxin-like half domain, PF01077: Nitrite | ||
| and sulphite reductase 4Fe—4S domain (68415.m01789) GDSL-motif | ||
| Inorganic N | At5g55050 | lipase/hydrolase family protein similar to family II lipases EXL3 GI: 15054386, |
| EXL1 GI: 15054382, EXL2 GI: 15054384 from | ||
| [Arabidopsis thaliana]; contains Pfam profile PF00657: GDSL-like | ||
| Lipase/Acylhydrolase (68418.m06861) | ||
| Inorganic N | At5g18600 | glutaredoxin family protein contains glutaredoxin domain, |
| INTERPRO: IPR002109 (68418.m02201) | ||
| Inorganic N | At1g49860 | glutathione S-transferase, putative similar to GI: 860955 from [Hyoscyamus |
| muticus] (Plant Physiol. 109 (1), 253-260 (1995)) (68414.m05590) | ||
| Inorganic N | At1g08090 | high-affinity nitrate transporter (ACH1) identical to trans-membrane nitrate |
| transporter protein AtNRT2: 1 [Arabidopsis thaliana] GI: 3747058, | ||
| high-affinity nitrate transporter ACH1 [Arabidopsis thaliana] GI: 3608362 | ||
| (68414.m00885) | ||
| Inorganic N | At1g12940 | high-affinity nitrate transporter, putative similar to trans-membrane nitrate |
| transporter protein AtNRT2: 1 [Arabidopsis thaliana] GI: 3747058, | ||
| high-affinity nitrate transporter ACH1 [Arabidopsis thaliana] GI: 3608362 | ||
| (68414.m01503) | ||
| Inorganic N | At1g12110 | nitrate/chlorate transporter (NRT1.1) (CHL1) identical to nitrate/chlorate |
| transporter SP: Q05085 from [Arabidopsis thaliana]; contains Pfam | ||
| profile: PF00854 POT family (68414.m01402) | ||
| Organic and | At1g55920 | serine O-acetyltransferase, putative identical to GI: 608677 from [Arabidopsis |
| Inorganic N | thaliana] (68414.m06414) | |
| Organic and | At2g16660 | nodulin family protein similar to nodulin-like protein [Arabidopsis thaliana] |
| Inorganic N | GI: 3329368, nodule-specific protein Nlj70 [Lotus japonicus] | |
| GI: 3329366 (68415.m01912) | ||
| Organic and | At3g45140 | lipoxygenase (LOX2) identical to SP |
| Inorganic N | ||
| Organic and | At5g64410 | oligopeptide transporter OPT family protein similar to SP |
| Inorganic N | ||
| Organic and | At5g65010 | asparagine synthetase 2 (ASN2) identical to asparagine synthetase (ASN2) |
| Inorganic N | [Arabidopsis thaliana] GI: 3859536 (68418.m08178) | |
| Organic N | At1g14780 | expressed protein (68414.m01767) |
| Organic N | At1g22160 | senescence-associated protein-related similar to senescence-associated protein |
| SAG102 (GI: 22331931) [Arabidopsis thaliana] | ||
| (68414.m02770) | ||
| Organic N | At1g31770 | ABC transporter family protein contains Pfam profile: PF00005: ABC transporter |
| (68414.m03899) | ||
| Organic N | At1g32450 | proton-dependent oligopeptide transport (POT) family protein contains Pfam |
| profile: PF00854 POT family (68414.m04005) | ||
| Organic N | At1g47128 | cysteine proteinase (RD21A)/thiol protease identical to SP |
| Organic N | At1g49500 | expressed protein (68414.m05548) |
| Organic N | At1g56150 | auxin-responsive family protein similar to SP: P33082 Auxin-induced protein |
| X15. [Soybean] {Glycine max} (68414.m06450) | ||
| Organic N | At1g67910 | expressed protein (68414.m07755) |
| Organic N | At1g74090 | sulfotransferase family protein similar to SP |
| Organic N | At1g74710 | isochorismate synthase 1 (ICS1)/isochorismate mutase identical to GI: 17223087 |
| and GB: AF078080; contains Pfam profile PF00425: chorismate binding enzyme; | ||
| contains TIGRfam profile TIGR00543: isochorismate synthases; identical to | ||
| cDNA isochorismate synthase 1 | ||
| precursor (ICS1) nuclear gene for plastid product GI: 17223086 (68414.m08655) | ||
| Organic N | At1g77760 | nitrate reductase 1 (NR1) identical to SP |
| Organic N | At1g78000 | sulfate transporter (Sultr1; 2) identical to sulfate transporter Sultr1; 2 [Arabidopsis |
| thaliana] GI: 7768660; contaisn Pfam profiles PF00916: | ||
| Sulfate transporter family and PF01740: STAS domain; contains TIGRfam | ||
| profile TIGR00815: sulfate permease (68414.m09090) | ||
| Organic N | At2g15970 | cold-acclimation protein, putative (FL3-5A3) similar to cold acclimation |
| Organic N | At2g27830 | WCOR413-like protein gamma form [Hordeum vulgare] gi |
| expressed protein (68415.m03374) | ||
| Organic N | At2g28550 | AP2 domain-containing transcription factor RAP2.7 (RAP2.7) nearly identical to |
| AP2 domain transcription factor RAP2.7 (GI: 2281639) | ||
| [Arabidopsis thaliana] (68415.m03469) | ||
| Organic N | At2g30040 | protein kinase family protein contains protein kinase domain, Pfam: PF00069 |
| (68415.m03653) | ||
| Organic N | At2g31790 | UDP-glucoronosyl/UDP-glucosyl transferase family protein contains Pfam |
| profile: PF00201 UDP-glucoronosyl and UDP-glucosyl transferase | ||
| (68415.m03881) | ||
| Organic N | At2g33710 | AP2 domain-containing transcription factor family protein similar to RAP2.6 |
| (GI: 17065542) {Arabidopsis thaliana} (68415.m04132) | ||
| Organic N | At2g33830 | dormancy/auxin associated family protein contains Pfam profile: PF05564 |
| dormancy/auxin associated protein (68415.m04151) | ||
| Organic N | At2g35930 | U-box domain-containing protein similar to immediate-early fungal elicitor |
| protein CMPG1 [Petroselinum crispum] GI: 14582200; contains Pfam | ||
| profile PF04564: U-box domain (68415.m04410) | ||
| Organic N | At2g39200 | seven transmembrane MLO family protein/MLO-like protein 12 (MLO12) |
| identical to SP | ||
| Organic N | At2g39570 | ACT domain-containing protein contains Pfam ACT domain PF01842 |
| (68415.m04854) | ||
| Organic N | At2g40140 | zinc finger (CCCH-type) family protein contains Pfam domain, PF00642: Zinc |
| finger C-x8-C-x5-C-x3-H type (and similar) and Pfam domain, | ||
| PF00023: Ankyrin repeat (68415.m04937) | ||
| Organic N | At2g43100 | aconitase C-terminal domain-containing protein contains Pfam profile PF00694: |
| Aconitase C-terminal domain (68415.m05350) | ||
| Organic N | At3g02910 | expressed protein contains Pfam domain PF03674: Uncharacterised protein |
| family (UPF0131) (68416.m00286) | ||
| Organic N | At3g05200 | zinc finger (C3HC4-type RING finger) family protein (ATL6) contains Pfam |
| profile: PF00097: Zinc finger, C3HC4 type (RING finger) | ||
| (68416.m00567) | ||
| Organic N | At3g10520 | non-symbiotic hemoglobin 2 (HB2) (GLB2) identical to SP |
| Organic N | At3g13930 | dihydrolipoamide S-acetyltransferase, putative similar to dihydrolipoamide S- |
| acetyltransferase [Zea mays] GI: 5669871; contains Pfam profiles PF00198: 2-oxo | ||
| acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin- | ||
| requiring enzyme, PF02817: e3 binding domain | ||
| (68416.m01759) | ||
| Organic N | At3g14940 | phosphoenolpyruvate carboxylase, putative/PEP carboxylase, putative strong |
| similarity to SP | ||
| Organic N | At3g15630 | expressed protein (68416.m01982) |
| Organic N | At3g47520 | malate dehydrogenase [NAD], chloroplast (MDH) identical to chloroplast NAD- |
| malate dehydrogenase [Arabidopsis thaliana] GI: 3256066; | ||
| contains InterPro entry IPR001236: Lactate/malate dehydrogenase; contains | ||
| Pfam profiles PF00056: lactate/malate dehydrogenase, NAD | ||
| binding domain and PF02866: lactate/malate dehydrogenase, alpha/beta C- | ||
| terminal domain (68416.m05168) | ||
| Organic N | At3g48740 | nodulin MtN3 family protein similar to MtN3 GI: 1619602 (root nodule |
| development) from [Medicago truncatula] (68416.m05322) | ||
| Organic N | At3g48990 | AMP-dependent synthetase and ligase family protein similar to peroxisomal- |
| coenzyme A synthetase (FAT2) [gi: 586339] from Saccharomyces cerevisiae; | ||
| contains Pfam AMP-binding enzyme domain PF00501; identical to cDNA; | ||
| identical to cDNA adenosine monophosphate binding | ||
| protein 3 AMPBP3 (AMPBP3)GI: 20799714 (68416.m05351) | ||
| Organic N | At3g49940 | LOB domain protein 38/lateral organ boundaries domain protein 38 (LBD38) |
| identical to SP | ||
| Organic N | At3g58990 | aconitase C-terminal domain-containing protein contains Pfam profile PF00694: |
| Aconitase C-terminal domain (68416.m06575) | ||
| Organic N | At3g60750 | transketolase, putative strong similarity to transketolase 1 [Capsicum annuum] |
| GI: 3559814; contains Pfam profiles PF02779: Transketolase, pyridine binding | ||
| domain, PF02780: Transketolase, C-terminal domain, PF00456: Transketolase, | ||
| thiamine diphosphate binding domain | ||
| (68416.m06796) | ||
| Organic N | At3g61190 | BON1-associated protein 1 (BAP1) identical to BON1-associated protein 1 |
| [Arabidopsis thaliana] GI: 15487384; contains Pfam profile PF00168: | ||
| C2 domain; supporting cDNA gi | ||
| Organic N | At3g61890 | homeobox-leucine zipper protein 12 (HB-12)/HD-ZIP transcription factor 12 |
| identical to homeobox-leucine zipper protein ATHB-12 | ||
| (GI: 6899887) [Arabidopsis thaliana] (68416.m06951) | ||
| Organic N | At4g12280 | copper amine oxidase family protein contains Pfam domain, PF01179: Copper |
| amine oxidase, enzyme domain (68417.m01946) | ||
| Organic N | At4g13510 | ammonium transporter 1, member 1 (AMT1.1) identical to SP |
| Organic N | At4g13770 | cytochrome P450 family protein (68417.m02136) |
| Organic N | At4g24620 | glucose-6-phosphate isomerase, putative similar to glucose-6-phosphate |
| isomerase [Spinacia oleracea] GI: 3413511; contains Pfam profile | ||
| PF00342: glucose-6-phosphate isomerase (68417.m03526) | ||
| Organic N | At4g30470 | cinnamoyl-CoA reductase-related similar to cinnamoyl-CoA reductase from |
| Pinus taeda [GI: 17978649], Saccharum officinarum [GI: 3341511] | ||
| (68417.m04326) | ||
| Organic N | At4g36670 | mannitol transporter, putative similar to mannitol transporter [Apium graveolens |
| var. dulce] GI: 12004316; contains Pfam profile PF00083: | ||
| major facilitator superfamily protein (68417.m05203) | ||
| Organic N | At4g37540 | LOB domain protein 39/lateral organ boundaries domain protein 39 (LBD39) |
| identical to SP | ||
| Organic N | At4g37610 | TAZ zinc finger family protein/BTB/POZ domain-containing protein contains |
| Pfam PF00651: BTB/POZ domain; contains Pfam PF02135: | ||
| TAZ zinc finger; similar to Speckle-type POZ protein (SP: O43791) [Homo | ||
| sapiens] (68417.m05321) | ||
| Organic N | At4g38470 | protein kinase family protein similar to protein kinase [gi: 170047] from Glycine |
| max; contains Pfam protein kinase domain PF00069 | ||
| (68417.m05436) | ||
| Organic N | At4g39800 | inositol-3-phosphate synthase isozyme 1/myo-inositol-1-phosphate synthase 1/ |
| MI-1-P synthase 1/IPS 1 identical to SP | ||
| Organic N | At5g04950 | nicotianamine synthase, putative similar to nicotianamine synthase [Lycopersicon |
| esculentum][GI: 4753801], nicotianamine synthase 2 | ||
| [Hordeum vulgare][GI: 4894912] (68418.m00524) | ||
| Organic N | At5g40850 | urophorphyrin III methylase (UPM1) identical to urophorphyrin III methylase |
| (GI: 1146165) [Arabidopsis thaliana]; similar to s-adenosyl-L-methionine- | ||
| dependent uroporphyrinogen III methyltransferase (GI: 1490606) [Arabidopsis | ||
| thaliana]; similar to Diphthine synthase (Diphtamide biosynthesis | ||
| methyltransferase) (DPH5) (SP: P32469) [Saccharomyces cerevisiae]; contains | ||
| Pfam PF00590: Tetrapyrrole (Corrin/Porphyrin) | ||
| Methylases domain; contains TIGRFAM PF00590: Tetrapyrrole | ||
| (Corrin/Porphyrin) Methylases (68418.m04960) | ||
| Organic N | At5g41670 | 6-phosphogluconate dehydrogenase family protein contains Pfam profiles: |
| PF00393 6-phosphogluconate dehydrogenase C-terminal domain, | ||
| PF03446 NAD binding domain of 6-phosphogluconate (68418.m05063) | ||
| Organic N | At5g45340 | cytochrome P450 family protein similar to SP |
| Organic N | At5g46050 | proton-dependent oligopeptide transport (POT) family protein contains Pfam |
| profile: PF00854 POT family (68418.m05663) | ||
| Organic N | At5g48370 | thioesterase family protein similar to SP |
| Organic N | At5g49480 | sodium-inducible calcium-binding protein (ACP1)/sodium-responsive calcium- |
| binding protein (ACP1) identical to NaCl-inducible Ca2+-binding | ||
| protein GI: 2352828 from [Arabidopsis thaliana] (68418.m06123) | ||
| Organic N | At5g51830 | pfkB-type carbohydrate kinase family protein contains Pfam profile: PF00294 |
| pfkB family carbohydrate kinase (68418.m06426) | ||
| Organic N | At5g54170 | expressed protein weak similarity to SP |
| Organic N | At5g58140 | protein kinase family protein/non phototropic hypocotyl 1-like protein (NPL1) |
| contains Pfam domains, PF00069: Protein kinase domain and PF00785: PAC | ||
| motif; similar to SP: O48963 Nonphototropic hypocotyl protein 1 (Phototropin) | ||
| [Mouse-ear cress] {Arabidopsis thaliana}; | ||
| identical to cDNA non phototropic hypocotyl 1-like (NPL1) GI: 5391441 | ||
| (68418.m07277) | ||
This table contains a list of genes that were previously identified as regulated by inorganic N (Wang et al., 2004 Plant Physiol 136:2512-2522) and that were found regulated in the current study. The first column summarizes the regulatory pattern observed in this study.
Nitrogen signals control amino acid metabolism in Arabidopsis seedlings. To evaluate the biological significance of the observed patterns of response to the treatments, the distribution of functional categories in the six classes defined in Table 3 were analyzed using the BioMaps program (Gutierrez et al., 2007, Genome Biol. 8:R7). To focus on the most prominent biological processes affected, over-represented functional terms (p≦0.01) with 5 or more genes (Table 5) were analyzed. This analysis indicated that inorganic N represses amino acid biosynthesis, and in particular a subset of genes related to the metabolism of S-containing amino acids. In addition, increased levels of internal Glu appears to induce the expression of genes involved in cell wall biosynthesis, especially genes in the xyloglucan:xyloglucosyl transferase family. Internal Glu also appears to repress genes involved in several aspects of metabolism, most prominently amino acid and carbohydrate metabolism. This analysis also showed that genes involved in secondary metabolism are repressed by both internal and external organic N sources. These results indicate that the balance between organic and inorganic N controls the expression of genes involved in N-reduction, N-assimilation and amino acid metabolism in Arabidopsis plants and coordinates N-assimilation with cellular processes including for example, cell wall biosynthesis.
| TABLE 5 | |||
| Response | MIPS Functional Term | p-value | Genes |
| Inorganic nitrogen repression | amino acid biosynthesis (12) | 0.00322 | At3g54640, At4g13890, At3g01120, At5g37600, At3g23810, |
| (100) | At2g36880, At4g39980, At4g15560, At5g23020, At3g17390, | ||
| At5g16570, At3g03780 | |||
| Internal Glu induction (89) | cell wall (7) | 0.00933 | At5g64570, At1g03870, At1g32170, At2g01850, At1g11545, |
| At3g23730, At3g14310 | |||
| Internal Glu repression (246) | METABOLISM (98) | 6.95E−05 | At3g61190, At5g65620, At5g64440, At5g37990, At3g02360, |
| At1g08920, At5g49720, At1g75680, At2g44160, At2g30490, | |||
| At5g35170, At1g77760, At4g00370, At1g51680, At1g15130, | |||
| At3g19420, At4g33580, At1g17840, At3g60750, At2g30870, | |||
| At3g54690, At1g15950, At2g47180, At5g01820, At5g20070, | |||
| At4g24620, At2g46830, At5g05730, At4g21850, At1g06640, | |||
| At5g20980, At5g48370, At4g36640, At4g34050, At2g38010, | |||
| At4g19810, At3g48690, At2g27860, At5g03555, At1g22610, | |||
| At2g36690, At3g63010, At3g21240, At4g29900, At2g39210, | |||
| At1g37130, At4g33680, At1g79380, At4g39800, At4g36250, | |||
| At5g63850, At1g02400, At1g53500, At1g66900, At5g01800, | |||
| At4g14440, At1g11840, At3g44720, At2g20360, At2g22240, | |||
| At5g54960, At4g12280, At5g55910, At1g03590, At2g38700, | |||
| At5g54160, At1g65960, At2g16430, At5g11110, At2g30040, | |||
| At4g11570, At3g21230, At5g58140, At4g39640, At5g37510, | |||
| At4g00360, At3g01560, At4g30440, At2g40890, At2g47880, | |||
| At3g48560, At5g49630, At1g76670, At5g43370, At4g30470, | |||
| At4g25300, At2g29450, At2g40140, At4g39330, At1g07890, | |||
| At4g30280, At1g12000, At2g36290, At5g53370, At1g74710, | |||
| At3g45640, At3g23820, At3g17820 | |||
| amino acid metabolism (28) | 0.00017 | At3g61190, At5g64440, At5g63850, At4g39640, At5g05730, | |
| At4g21850, At1g06640, At5g20980, At1g02400, At2g44160, | |||
| At3g48560, At5g49630, At2g30490, At1g11840, At1g77760, | |||
| At3g44720, At1g22610, At4g25300, At5g54960, At3g60750, | |||
| At2g36690, At1g07890, At3g54690, At1g37130, At1g65960, | |||
| At1g74710, At4g33680, At3g17820 | |||
| complex cofactor binding (13) | 0.00235 | At4g36250, At3g60750, At5g37510, At4g36640, At1g15950, | |
| At2g44160, At3g48560, At1g53500, At2g30490, At2g27860, | |||
| At4g33680, At5g54960, At4g12280 | |||
| C-compound and | 0.00673 | At4g36250, At3g02360, At5g49720, At1g75680, At2g44160, | |
| carbohydrate utilization (36) | At1g53500, At1g11840, At2g20360, At2g22240, At5g54960, | ||
| At4g33580, At3g60750, At3g54690, At1g15950, At5g11110, | |||
| At2g47180, At4g11570, At4g24620, At3g21230, At5g37510, | |||
| At5g20980, At4g30440, At3g48560, At3g48690, At2g27860, | |||
| At1g76670, At4g30470, At4g39330, At4g30280, At3g21240, | |||
| At3g63010, At2g36290, At1g12000, At5g53370, At3g23820, | |||
| At4g39800 | |||
| Internal/External Glu repression | secondary metabolism (19) | 0.0036 | At3g16150, At1g13110, At4g15390, At3g58990, At1g06000, |
| (102) | At4g39950, At4g22710, At4g30210, At1g20510, At2g05710, | ||
| At5g48010, At5g26030, At1g02500, At5g40850, At1g10360, | |||
| At1g05010, At2g34460, At5g47990, At5g45340 | |||
Network analysis reveals a metabolic gene network connected to regulatory transcription factors regulated by organic N. To uncover the mechanism underlying gene regulation in response to sensing Glu or a Glu-derived product, network analysis was used to identify the subnetwork of genes regulated by organic N (FIG. 3). The subnetwork of N-regulated genes using an Arabidopsis multinetwork was generated as described previously (Gutierrez et al., 2007, Genome Biol 8:R7). Cytoscape was used to visualize the resulting subnetworks wherein genes were represented as nodes connected by edges that represented distinct interactions (e.g., metabolic reactions, regulatory interactions). In addition to the interactions described previously (Gutierrez et al., 2007, Genome Biol 8:R7), regulatory connections were predicted between genes and associated transcription factors (see, Materials and Methods). In order to identify putative “master regulators” that control the expression of genes regulated by organic N, the transcription factors regulated in these experiments were ranked based on the number of regulatory connections in the subnetwork (Table 6). At the top of the list, were a Myb family transcription factor (At1g74840), the central clock gene CCA1 (At2g46830) and a golden 2-related transcription factor (GLK1; At2g20570). Interestingly, both CCA1 and GLK1 were predicted to positively affect the expression of a gene for glutamine synthetase (GLN1.3) (which uses Glu in a biosynthetic reaction), and to negatively affect the expression of a glutamate dehydrogenase gene (GDH1) (which catabolizes Glu) (FIG. 3). Moreover, the analysis suggests that both CCA1 and GLK1 block the expression of a bZIP transcription factor (bZIP1) which is predicted to induce the expression of the Gln-dependent ASN1 gene. Thus, Glu regulation of the GLK1 and CCA1 transcription factors appears to coordinate the expression of genes involved in making Gln (GLN1.3) vs. those involved in metabolizing Gln into Glu (ASN1, GDH1). In addition, another gene in this gene subnetwork encodes a putative asparaginase gene (ANS) that controls the degradation of Asn (to Asp and Glu) (FIG. 3). The ASN1 (Asn biosynthesis) and ANS (Asn degradation) genes had inverse expression patterns (correlation=−0.51), suggesting that Glu coordinates the reciprocal regulation of Asn synthesis and degradation by coordinating an antiregulation of the cognate.
| TABLE 6 | ||
| Number of | PUB | |
| connections | LOCUS | Annotation |
| 51 | At1g74840 | myb family transcription factor. |
| 47 | At2g46830 | myb-related transcription factor (CCA1) |
| 46 | At2g20570 | golden2-like transcription factor (GLK1) |
| 31 | At4g37260 | myb family transcription factor (MYB73) |
| 30 | At5g24800 | bZIP1 transcription factor family protein |
| contains | ||
| 30 | At5g49450 | bZIP1 family transcription factor |
| 29 | At5g14540 | proline-rich family protein contains proline |
| rich extensin domains. | ||
| 29 | At5g48655 | zinc finger (C3HC4-type RING finger) |
| family protein | ||
| 24 | At1g22070 | bZIP1 family transcription factor (TGA3) |
| 23 | At3g01560 | proline-rich family protein contains proline |
| rich extensin domains. | ||
| 20 | At2g22430 | homeobox-leucine zipper protein 6 (HB-6) |
| 19 | At5g44190 | myb family transcription factor (GLK2) |
| 16 | At1g43160 | AP2 domain-containing protein RAP2.6 |
| (RAP2.6) | ||
| 15 | At3g61890 | homeobox-leucine zipper protein 12 (HB-12) |
| 14 | At5g61890 | AP2 domain-containing transcription factor |
| family protein similar to RAP2.6 | ||
| 12 | At3g61150 | homeobox-leucine zipper family protein |
| 11 | At2g38470 | WRKY family transcription factor |
| 11 | At4g17500 | ethylene-responsive element-binding |
| protein 1 (ERF1) | ||
| 10 | At2g25000 | WRKY family transcription factor |
Validation of network model predictions highlights the regulatory role of CCA1 in the N-assimilatory pathway. The model in FIG. 3 predicts that CCA1 and/or GLK1 genes are important regulators of genes involved in N-assimilation and over-expression of either one of these genes would repress the expression of ASN1 and GDH1 and induce the expression of the GLN1.3 gene. Conversely, a knockout of the CCA1 or GLK1 gene should increase ASN1 and GDH1 expression levels and diminish GLN1.3 mRNA levels. To test these hypotheses, we used previously characterized CCA1 overexpressor (CCA1-ox) (Wang et al., 1998, Cell 93:1207-1217) and GLK1 gene knockout (glk1) (Fitter et al., 2002, Plant J 31:713-727) lines. A stronger phenotype for the overexpressor lines was anticipated as compared to the knockout, as the model predicts redundancy in the function of CCA1 and GLK1 in regulating ASN1, GDH1 and GLN1.3 gene expression. CCA1-ox, glk1 and wild-type plants were grown for two weeks as above, and samples were collected in the morning (3 h after dawn). Total RNA was extracted from whole seedlings and RT-qPCR was performed to determine mRNA levels for ASN1, GLN1.3 and GDH1 in the three genotypes. As shown in FIG. 4, all three genes tested showed altered expression patterns in the mutant lines utilized (as determined by analysis of variance, p≦0.05) which were consistent with the predicted network model shown in FIG. 1. In addition, bZIP1 mRNA level was also repressed in CCA1-ox (FIG. 10). ASN1, GDH1 and GLN1.3 mRNA levels were not altered in the glk1 line, with the exception of a small increase in GDH1 mRNA levels. This is probably due to the redundant function of GLK1 and CCA1 in regulating the expression of the tested genes. In contrast, and as predicted by the model shown in FIG. 3, ASN1 and GDH1 levels were decreased in the CCA1-ox line. Also consistent with the predictions of the model, GLN1.3 mRNA levels were increased as compared to wild-type in the CCA1-ox.
The network model predicts that the effect of CCA1 on the expression of the target genes will be direct. To test this hypothesis, ChIP assays were used using a CCA1 antibody (FIG. 4B). As controls, it was demonstrated that the ChIP assays could detect binding of CCA1 protein to a region of the TOC1 promoter, a known target of CCA1, but was not able to detect the ZTL promoter which has no circadian oscillation at the mRNA level. Consistent with the model for CCA1, ChIP assays in both wild-type and CCA1-ox lines were able to confirm binding of CCA1 to the promoter regions of GLN1.3, GDH1 and bZIP1 promoters. These results support the model, and indicate that CCA1 regulates expression of bZIP1, GDH1 and GLN1.3 genes directly, and indirectly for ASN1 through bZIP1.
N-nutrient signals act as input to the Arabidopsis circadian clock. CCA1 is a key component of a negative feedback loop at the center of the Arabidopsis circadian clock (McClung, 2006, Plant Cell 18:792-803; Millar, 2004, J. Exp. Bot. 55:277-283). Because the results showed that N-treatments affected CCA1 expression, it was hypothesized that N might serve as an input capable of affecting the circadian clock function. To test this hypothesis, pulses of inorganic or organic N were provided at intervals spanning a circadian cycle and determined the effects on the phase of the oscillation in CCA1::LUC expression. Each treatment resulted in stable phase shifts indicating that N status serves as an input to the circadian clock (FIG. 5 and FIG. 11). Inorganic N and 10 mM Glu treatments conferred slight phase advances whereas 10 mM Gln conferred only delays. The Nms and Glu pulses did not affect the period but the Gln pulse shortened the period as determined by one-way analysis of variance and Dunn's multiple comparison tests. Thus, the clock regulates a number of steps in N metabolism, such as NR expression and activity (Pilgrim et al., 1993, Plant Mol Biol 23:349-64) and ASN1 expression as demonstrated herein (see also Harmer et al., 2000, Science 290:2110-2113). In turn, N status feeds back to the clock, at least in part through its effect on CCA1 expression.
7.4 Discussion
In the present study, genomic and pharmacological approaches were used to distinguish organic from inorganic N responses in Arabidopsis seedlings. The majority of the genes regulated by the N-treatments used in this study (81%) are responding to organic N signals. Among the genes regulated by organic N, we distinguished two classes of genes: (i) genes that responded only to external Glu application and (ii) genes that responded to internal and external sources of Glu (Table 3). The difference in these two expression patterns raises the possibility that there are different mechanisms for sensing internally produced cellular Glu vs. extra cellular Glu that is transported between cells. The other possibility is that the differences in internal vs. external Glu responses observed in this study reflect distinct threshold responses to Glu levels. There is precedence for internal and external Glu sensing mechanisms in other organisms. Bacteria regulate ammonium assimilation via a mechanism involving PII, a sensor that measures levels of α-ketoglutarate and Glu (Arcondeguy et al., 2001, Microbiol Mol Biol Rev 65:80-105). Plants contain a PII protein that is localized to chloroplasts (Hsieh et al., 1998, Proc Natl Acad Sci USA 95:13965-13970), a potential sensor of internal levels of Glu. By contrast, extracellular Glu is sensed by Glu receptors in animal brains (Sykova, 2004, Neuroscience 129:861-876). The presence of Glu receptor genes in plants (Lacombe et al., 2001, Science 292:1486-1487) raises the possibility that Glu receptors in plants may serve to sense levels of external apoplastic transported Glu.
Analysis of the genes regulated by N, identified a gene network with transcription factors that appear to regulate the expression of N-assimilatory genes. New to this study is the finding that the NR genes (NIA1, NIA2) are repressed by organic N, as is GLN1.3, which is involved in Gln biosynthesis. Within this N-regulated network we also found genes involved in N uptake and metabolism including an ammonium transporter (AMT1.1), genes involved in assimilating N into and out of Asn (ASN1, ANS), as well as amino acid transporter genes. Organic N negatively regulated AMT1.1 and ANS, but induced the ASN1 gene. It was therefore hypothesized that in the presence of Glu, or a Glu-derived metabolite, Asn production is optimized and regulated at the level of transcription by increasing levels of ASN1 and decreasing levels of ANS transcripts. These results are consistent with Asn serving as a major N storage compound (Lam et al., 1994, Plant Physiol 106:1347-1357) controlled by the ASN1 gene, and suggest a mechanism to maximize Asn production, degradation and distribution depending on levels of internal sources of organic N.
The network analysis proposed a mechanism for transcriptional regulation of N-assimilation. ASN1 was a predicted target of the transcription factor bZIP1; GDH1, GLN1.3 and bZIP1 were predicted targets of GLK1 and CCA1. Because bZIP1 is also regulated by carbon (Gutierrez et al., 2007, Genome Biol 8:R7), this gene may be an integrator of C and N signaling for regulation of N-assimilation in Arabidopsis. This our network model was validated by measuring mRNA levels of the target genes in CCA1-ox and glk1 knockout lines. As predicted, ASN1 and GDH1 mRNA levels were down regulated and GLN1.3 mRNA was elevated in the CCA1-ox line. In contrast, mRNA levels for these three genes were not affected in the glk1 knockout line. The lack of a molecular phenotype in the glk1 knockout may be explained by the fact that CCA1 and GLK1 are predicted to have the same regulatory function in the subnetwork. The predictions that CCA1 directly targets a number of genes in the network was validated using CCA1 antibodies in ChIP experiments. Because CCA1 is one of the central components of the circadian clock in Arabidopsis, regulation of CCA1 expression in response to organic N suggests that the circadian clock may receive N nutritional inputs in plants. Thus, in addition to light and temperature (Millar, 2004, J. Exp. Bot. 55:277-283; McClung, 2001, Ann. Rev. Plant Physiol Plant Mol. Biol. 52:139-162), nutrients—such as N—may act as input for the clock. The phase response curve analysis results presented herein are consistent with weak (type 1) resetting similar to those observed in response to light pulses in Lemna gibba (Kondo, 1983, Plant Cell Physiol. 24:659-665), KCl or ethanol pulses in Phaseolus coccineus (Bunning and Moser, 1973, Proc Natl Acad Sci USA 70:3387-3389) and cAMP or imidazole pulses in Trifolium repens (Bollig et al., 1978, Planta 141:225-230), strengthening the hypothesis that N status feeds back to the clock, at least in part through its effect on CCA1 expression. In Arabidopsis, light pulses evoke strong delays (˜8 h) in the early night and strong advances (5-10 h) later in the night (Covington et al., 2001, Plant Cell 13:1305-1315). Although the molecular basis of these phase shifts is not definitively established, they may involve induction of CCA1 by light (Wang et al., 1998, Cell 93:1207-1217). In the above-experiments, N treatment would decrease CCA1 mRNA abundance. That this elicits only small phase shifts suggests that posttranscriptional regulation buffers against CCA1 activity changes from reduced mRNA, at least over the time frames tested with our 4-h N pulses. Alternatively, N treatment may also modulate other clock components either at the mRNA, protein abundance or protein activity level in ways that reduce the magnitude of the phase shifts in response to CCA1 mRNA decrease. The emerging view of the circadian clock is as a key integrator of multiple metabolic and physiologic processes (Lam et al., 1994, Plant Physiol 106:1347-1357; Kondo, 1983, Plant Cell Physiol. 24:659-665). As such it receives input not only from environmental stimuli but also from multiple metabolic pathways, many of which are subject to circadian regulation. Thus, the clock regulates a number of steps in N metabolism, such as NR expression and activity (Bunning and Moser, 1973, Proc Natl Acad Sci USA 70:3387-3389) and ASN1 expression. In turn, N status feeds back to the clock, at least in part through its effect on CCA1 expression. This feedback is more subtle than the effects of saturating light pulses and our results are consistent with N status fine tuning clock function rather than conferring large changes such as those observed in response to light (FIG. 6).
Oscillations in the mRNA of genes that code for metabolic enzymes could have an impact on metabolite levels, as recently shown (Gibon et al., 2006, Genome Biology 7:R76). Predicting time of food availability is key for the survival in most animals (Stephan, 2002, J Biol Rhythms 17:284-292). The data presented herein suggest that this may also be the case in Arabidopsis, e.g., anticipating the availability of carbon skeletons produced by photosynthesis to assimilate inorganic N into amino acids. Moreover, this data provides a plausible molecular mechanism for how this could happen in plants via CCA1. The present study thus provides evidence that plant nutrition, like in animals, is tightly linked to circadian functions as previously hypothesized (Harmer et al., 2000, Science 290:2110-2113). Recently, it was shown that the central clock gene Per2 is necessary for food anticipation in mice (Feillet et al., 2006, Curr Biology 16:2016-2022). The present data indicates that the central clock gene CCA1 plays a role in circadian regulation of N-assimilation in plants (FIG. 12). This data is consistent with a model in which the N-assimilatory pathway is a downstream target of the clock with CCA1 being the direct regulatory factor. Moreover, Glu or other Glu-derived signal act as input to the circadian clock providing a link between plant N-nutrition and circadian rhythms.
8. EQUIVALENTS
Although the invention is described in detail with reference to specific embodiments thereof, it will be understood that variations which are functionally equivalent are within the scope of this invention. Indeed, various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description and accompanying drawings. Such modifications are intended to fall within the scope of the appended claims. Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.
All publications, patents and patent applications mentioned in this specification are herein incorporated by reference into the specification to the same extent as if each individual publication, patent or patent application was specifically and individually indicated to be incorporated herein by reference in their entireties.
| Annex: Expression patterns in response to the Nms, Nms MSX, and Nms MSX Glu treatments. | |||||
| Pattern or | |||||
| response to | |||||
| the | |||||
| treatments | |||||
| Nms-Nms/ | |||||
| MSX- | |||||
| # | Class and interpretation | Name/MSX | ProbeID | AGI locus | Gene annotation |
| 1 | (A) Inorganic Nitrogen | I-I-I | 267293_at | At2g23810 | senescence-associated family protein similar to senescence-associated protein 5 [Hemerocallis hybrid cultivar] |
| induction | |||||
| 2 | (A) Inorganic Nitrogen | I-I-I | 267028_at | At2g38470 | WRKY family transcription factor contains Pfam profile: PF03106 WRKY DNA-binding domain; (68415.m04725) |
| induction | |||||
| 3 | (A) Inorganic Nitrogen | I-I-I | 266800_at | At2g22880 | VQ motif-containing protein contains PF05678: VQ motif (68415.m02716) |
| induction | |||||
| 4 | (A) Inorganic Nitrogen | I-I-I | 266097_at | At2g37970 | SOUL heme-binding family protein weak similarity to SOUL protein [Mus musculus] GI: 4886906; contain Pfam profile PF04832: |
| induction | SOUL heme-binding protein (68415.m04660) | ||||
| 5 | (A) Inorganic Nitrogen | I-I-I | 265475_at | At2g15620 | ferredoxin-nitrite reductase, putative strong similarity to ferredoxin--nitrite reductase [Nicotiana tabacum] GI: 19893; contains Pfam |
| induction | profiles PF03460: Nitrite/Sulfite reductase ferredoxin-like half domain, PF01077: Nitrite and sulphite reductase 4Fe—4S domain | ||||
| (68415.m01789) | |||||
| 6 | (A) Inorganic Nitrogen | I-I-I | 265075_at | At1g55450 | embryo-abundant protein-related similar to embryo-abundant protein GI: 1350531 from [Picea glauca] (68414.m06342) |
| induction | |||||
| 7 | (A) Inorganic Nitrogen | I-I-I | 264953_at | At1g77120 | alcohol dehydrogenase (ADH) identical to alcohol dehydrogenase GI: 469467 from (Arabidopsis thaliana) (68414.m06982) |
| induction | |||||
| 8 | (A) Inorganic Nitrogen | I-I-I | 264636_at | At1g65490 | expressed protein (68414.m07431) |
| induction | |||||
| 9 | (A) Inorganic Nitrogen | I-I-I | 264580_at | At1g05340 | expressed protein (68414.m00541) |
| induction | |||||
| 10 | (A) Inorganic Nitrogen | I-I-I | 263475_at | At2g31945 | expressed protein (68415.m03902) |
| induction | |||||
| 11 | (A) Inorganic Nitrogen | I-I-I | 263182_at | At1g005575 | expressed protein (68414.m00576) |
| induction | |||||
| 12 | (A) Inorganic Nitrogen | I-I-I | 262832_s_at | At1g14870 | expressed protein similar to PGPS/D12 [Petunia x hybrida] GI: 4105794; contains Pfam profile PF04749: Protein of unknown |
| induction | function, DUF614 (68414.m01778) | ||||
| 13 | (A) Inorganic Nitrogen | I-I-I | 262571_at | At1g15430 | expressed protein (68414.m01852) |
| induction | |||||
| 14 | (A) Inorganic Nitrogen | I-I-I | 262238_at | At1g48300 | expressed protein (68414.m05395) |
| induction | |||||
| 15 | (A) Inorganic Nitrogen | I-I-I | 261892_at | At1g80840 | WRKY family transcription factor similar to WRKY transcription factor GB: BAA87058 GI: 6472585 from [Nicotiana tabacum] |
| induction | (68414.m09484) | ||||
| 16 | (A) Inorganic Nitrogen | I-I-I | 261749_at | At1g76180 | dehydrin (ERD14) identical to SP|P42763 Dehydrin ERD14 {Arabidopsis thaliana} (68414.m08846) |
| induction | |||||
| 17 | (A) Inorganic Nitrogen | I-I-I | 261648_at | At1g27730 | zinc finger (C2H2 type) family protein (ZAT10)/salt-tolerance zinc finger protein (STZ) identical to salt-tolerance zinc finger protein |
| induction | GB: CAA64820 GI: 1565227 from [Arabidopsis thaliana]; contains Pfam domain, PF00096: Zinc finger, C2H2 type; identical to cDNA | ||||
| zinc finger protein STZ/ZAT10 (STZ/ZAT10) GI: 9965730 (68414.m03389) | |||||
| 18 | (A) Inorganic Nitrogen | I-I-I | 261193_at | At1g32920 | expressed protein (68414.m04055) |
| induction | |||||
| 19 | (A) Inorganic Nitrogen | I-I-I | 260522_x_at | At2g41730 | expressed protein (68415.m05158) |
| induction | |||||
| 20 | (A) Inorganic Nitrogen | I-I-I | 260101_at | At1g73260 | trypsin and protease inhibitor family protein/Kunitz family protein similar to trypsin inhibitor propeptide [Brassica oleracea] |
| induction | GI: 841208; contains Pfam profile PF00197: Trypsin and protease inhibitor (68414.m08478) | ||||
| 21 | (A) Inorganic Nitrogen | I-I-I | 259979_at | At1g76800 | expressed protein (68414.m08913) |
| induction | |||||
| 22 | (A) Inorganic Nitrogen | I-I-I | 259705_at | At1g77450 | no apical meristem (NAM) family protein contains Pfam PF02385: No apical meristem (NAM) domain; similar to GRAB1 protein |
| induction | GB: CAA09371, a novel member of the NAC domain family (68414.m09019) | ||||
| 23 | (A) Inorganic Nitrogen | I-I-I | 259570_at | At1g20440 | dehydrin (COR47) identical to dehydrin COR47 (Cold-induced COR47 protein) [Arabidopsis thaliana] SWISS-PROTT: P31168 |
| induction | (68414.m02547) | ||||
| 24 | (A) Inorganic Nitrogen | I-I-I | 259479_at | At1g19020 | expressed protein (68414.m02367) |
| induction | |||||
| 25 | (A) Inorganic Nitrogen | I-I-I | 259037_at | At3g09350 | armadillo/beta-catenin repeat family protein contains Pfam profile: PF00514 armadillo/beta-catenin-like repeat (68414.m01109) |
| induction | |||||
| 26 | (A) Inorganic Nitrogen | I-I-I | 257206_at | At3g16530 | leguma lectin family protein contains Pfam domain, PF00139: Legume lectins beta domain (68416.m02111) |
| induction | |||||
| 27 | (A) Inorganic Nitrogen | I-I-I | 256337_at | At1g72060 | expressed protein (68414.m08330) |
| induction | |||||
| 28 | (A) Inorganic Nitrogen | I-I-I | 255543_at | At4g01870 | tolB protein-related contains weak similarity to TolB protein precursor (Swiss-Prot: P44677) [Haemophilus influenzae] |
| induction | (68417.m00245) | ||||
| 29 | (A) Inorganic Nitrogen | I-I-I | 254926_at | At4g11280 | 1-aminocyclopropane-1-carboxylate synthase 6/ACC synthase 6 (ACS6) identical to GI: 3746125 (68417.m01824) |
| induction | |||||
| 30 | (A) Inorganic Nitrogen | I-I-I | 254158_at | At4g24380 | expressed protein contains Pfam profile: PF03959 domain of unknown function (DUF341) (68417.m03496) |
| induction | |||||
| 31 | (A) Inorganic Nitrogen | I-I-I | 253915_at | At4g27280 | calcium-binding EF hand family protein similar to EF-hand Ca2+-binding protein CCD1 [Triticum aestivum] GI: 9255753; contains |
| induction | INTERPRO: IPR002048 calcium-binding EF-hand domain (68417.m03915) | ||||
| 32 | (A) Inorganic Nitrogen | I-I-I | 253872_at | At4g27410 | no apical meristem (NAM) family protein (RD26) contains Pfam PF02365: No apical meristem (NAM) domain; Arabidopsis thaliana |
| induction | nap gene, PID: e1234813; identical to cDNA RD26 mRNA for NAM-like protein GI: 1046270 fom [Arabidopsis thaliana] | ||||
| (68417.m05078) | |||||
| 33 | (A) Inorganic Nitrogen | I-I-I | 253161_at | At4g35770 | senescence-associated protein (SEN1) identical to senescence-associated protein GI: 1046270 from [Arabidopsis thaliana] |
| induction | (68417.m05078) | ||||
| 34 | (A) Inorganic Nitrogen | I-I-I | 253046_at | At4g37370 | cytochrome P450, putative similar to Cytochrome P450 91A1 (SP: Q9FG65)[Arabidopsis thaliana]; cytochrome P450, Glycyrrhiza |
| induction | echinata, AB001379 (68417.m05292) | ||||
| 35 | (A) Inorganic Nitrogen | I-I-I | 252908_at | At4g39670 | expressed protein (68417.m05610) |
| induction | |||||
| 36 | (A) Inorganic Nitrogen | I-I-I | 252671_at | At3g44190 | pyridine nucleotide-disulphide oxidoreductase family protein low similarity to dihydrolipoamide dehydrogenase from Clostridium |
| induction | magnum [GI: 472330]; contains Pfam profile PF00070 Pyridine nucleotide-disulphide oxidoreductase domain (68416.m04738) | ||||
| 37 | (A) Inorganic Nitrogen | I-I-I | 252234_at | At3g49780 | phytosulfokines 3 (PSK3) identical to phytosulfokines 3 (PSK3) SP: Q9M2Y0 from [Arabidopsis thaliana] (68418.m05443) |
| induction | |||||
| 38 | (A) Inorganic Nitrogen | I-I-I | 250781_at | At5g05410 | DRE-binding protein (DREB2A) identical to DREB2A GI: 3738230 from [Arabidopsis thaliana]; supported by |
| induction | cDNA: gi_3738229_dbj_AB007790.1 AB007790 (68418.m00583) | ||||
| 39 | (A) Inorganic Nitrogen | I-I-I | 250351_at | At5g12030 | 17.7 kDa class II heat shock protein 17.6A (HSP17.7-CII) identical to heat shock protein 17.6A GI: 3256075 from [Arabidopsis |
| induction | thaliana] (68418.m01406) | ||||
| 40 | (A) Inorganic Nitrogen | I-I-I | 249996_at | At5g18600 | glutaredoxin family protein contains glutaredoxin domain, INTERPRO: IPR002109 (68418.m02201) |
| induction | |||||
| 41 | (A) Inorganic Nitrogen | I-I-I | 249719_at | At5g35735 | auxin-responsive family protein similar to auxin-induced protein AIR12 GI: 11357190 [Arabidopsis thaliana] (68418.m04276) |
| induction | |||||
| 42 | (A) Inorganic Nitrogen | I-I-I | 248790_at | At5g47450 | major intrinsic family protein/MIP family protein contains Pfam profile: MIP PF00230 (68418.m05853) |
| induction | |||||
| 43 | (A) Inorganic Nitrogen | I-I-I | 248164_at | At5g54490 | calcium-binding EF-hand protein, putative similar to EF-hand Ca2+-binding protein CCD1 [Triticum aestivum] GI: 9255753; contains |
| induction | INTERPRO: IPR002048 calcium-binding EF-hand domain (68418.m06785) | ||||
| 44 | (A) Inorganic Nitrogen | I-I-I | 248146_at | At5g54940 | eukaryotic translation initiation factor SUI1, putative similar to SP|P32911 Protein translation factor SUI1 {Saccharomyces |
| induction | cerevisiae}; contains Pfam profile PF01253: Translation initiation factor SUI1 (68418.m06842) | ||||
| 45 | (A) Inorganic Nitrogen | I-I-I | 247655_at | At5g59820 | zinc finger (C2H2 type) family protein (ZAT12) identical to zinc finger protein ZAT12 [Arabidopsis thaliana] |
| induction | gi|1418325|emb|CAA67232 (68418.m07500) | ||||
| 46 | (A) Inorganic Nitrogen | I-I-I | 247488_at | At5g61820 | expressed protein MtN19, Medicago truncatula, EMBL: MTY15367 (68418.m07757) |
| induction | |||||
| 47 | (A) Inorganic Nitrogen | I-I-I | 247351_at | At5g63790 | no apical meristem (NAM) family protein contains Pfam PF02365: No apical meristem (NAM) domain; contains similarity to NAC- |
| induction | domain protein (68418.m08006) | ||||
| 48 | (A) Inorganic Nitrogen | I-I-I | 246744_at | At5g27760 | hypoxia-responsive family protein contains Pfam profile: PF04588 hypoxia induced protein conserved region (68418.m03329) |
| induction | |||||
| 49 | (A) Inorganic Nitrogen | I-I-I | 246293_at | At3g56710 | sigA-binding protein identical to SigA binding protein [Arabidopsis thaliana] gi|6980074|gb|AAF34713; contains Pfam PF05678: VQ |
| induction | motif (68416.m06308) | ||||
| 50 | (A) Inorganic Nitrogen | I-I-I | 246099_at | At5g20230 | plastocyanin-like domain-containing protein (68418.m02408) |
| induction | |||||
| 51 | (A) Inorganic Nitrogen | I-I-I | 245734_at | At1g73480 | hydrolase, alpha/beta fold family protein low similarity to monoglyceride lipase from [Homo sapiens] GI: 14594904, [Mus musculus] |
| induction | GI: 2632162; contains Pfam profile PF00561: hydrolase, alpha/beta fold family (68414.m08507) | ||||
| 52 | (A) Inorganic Nitrogen | I-I-I | 245711_at | At5g04340 | zinc finger (C2H2 type) family protein contains Pfam domain, PF00096: Zinc finger, C2H2 type (68416.m00426) |
| induction | |||||
| 53 | (A) Inorganic Nitrogen | I-I-I | 245250_at | At4g17490 | ethylene-responsive element-binding protein, putative similar to SP|O80341 Ethylene responsive element binding factor 5 (AtERF5) |
| induction | {Arabidopsis thaliana} (68417.m02617) | ||||
| 54 | (A) Inorganic Nitrogen | I-I-I | 245026_at | atpH | ATPase III subunit |
| induction | |||||
| 55 | (A) Inorganic Nitrogen | I-I-I | 245006_at | psaB | PSI P700 apoprotein A2 |
| induction | |||||
| 56 | (A) Inorganic Nitrogen | I-I-I | 245017_at | psaI | PSI I protein |
| induction | |||||
| 57 | (A) Inorganic Nitrogen | I-I-I | 244961_at | ycf5 | hypothetical protein |
| induction | |||||
| 58 | (A) Inorganic Nitrogen | I-I-I | 257536_at | At3g02800 | tyrosine specific protein phosphatase family protein contains tyrosine specific protein phosphatases active site, PROSITE: PS00383 |
| induction | (68416.m00272) | ||||
| 59 | (A) Inorganic Nitrogen | I-I-I | 257540_at | At3g21520 | expressed protein contains Pfam profile PF05078: Protein of unknown function (DUF679) (68416.m02715) |
| induction | |||||
| 60 | (A) Inorganic Nitrogen | D-D-D | 267422_at | At2g35050 + 1 | protein kinase family protein contains Pfam profile: PF00069 Eukeryotic protein kinase domain (68415.m04300) |
| repression | |||||
| 61 | (A) Inorganic Nitrogen | D-D-D | 266588_at | At2g14890 | arabinogalactan-protein (AGP9) identical to gi|10880495|gb|AAG24277 (68415.m01692) |
| repression | |||||
| 62 | (A) Inorganic Nitrogen | D-D-D | 266022_at | At2g05920 | subtilase family protein contains similarity to cucumisin-like serine protease GI: 3176874 from [Arabidopsis thaliana] |
| repression | (68415.m00642) | ||||
| 63 | (A) Inorganic Nitrogen | D-D-D | 265733_at | At2g01190 | octicosapeptide/Phox/Bem1p (PB1) domain-containing protein Pfam profile PF00564: PB1 domain (68415.m00030) |
| repression | |||||
| 64 | (A) Inorganic Nitrogen | D-D-D | 265400_at | At2g10940 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein similar to proline-rich cell wall protein [Medicago sativa] |
| repression | GI: 3818416; contains Pfam profile PF00234 Protease inhibitor/seed storage/LTP family (68415.m01167) | ||||
| 65 | (A) Inorganic Nitrogen | D-D-D | 265042_at | At1g04040 | acid phosphatase class B family protein similar to SP|P15490 STEM 28 kDa glycoprotein precursor (Vegetative storage protein A) |
| repression | {Glycine max}, acid phosphatase [Glycine max] GI: 3341443; contains Pfam profile PF03767: HAD superfamily (subfamily IIIB) | ||||
| phosphatase; supporting cDNA gi|13926197|gb|AF370572.1|AF370572 (68414.m00391) | |||||
| 66 | (A) Inorganic Nitrogen | D-D-D | 265028_at | At1g24530 | transducin family protein/WD-40 repeat family protein similar to Vegetatible incompatibility protein HET-E-1 (SP: Q00808) |
| repression | {Podospora anserina}; contains 7 WD-40 repeats (PF00400) (68414.m03088) | ||||
| 67 | (A) Inorganic Nitrogen | D-D-D | 264857_at | At1g24170 | glycosyl transferase family 6 protein contains Pfam profile: PF01501 glycosyl transferase family 8 (68414.m03049) |
| repression | |||||
| 68 | (A) Inorganic Nitrogen | D-D-D | 264348_at | At1g12110 | nitrate/chlorate transporter (NRT1.1) (CHL1) identical to nitrate/chlorate transporter SP: Q05085 from [Arabidopsis thaliana]; |
| repression | contains Pfam profile: PF00854 POT family (68414.m01402) | ||||
| 69 | (A) Inorganic Nitrogen | D-D-D | 264335_s_at | At1g70320 | ubiquitin-protein figase 2 (UPL2) nearly identical to ubiquitin-protein ligase 2 [Arabidopsis thaliana] GI: 7108523; E3, HECT-domain |
| repression | protein family; similar to ubiquitin-protein ligase 2 GI: 7108523 from [Arabidopsis thaliana] (68414.m08090) | ||||
| 70 | (A) Inorganic Nitrogen | D-D-D | 264309_at | At1g62020 | coatomer protein complex, subunit alpha, putative contains Pfam PF00400: WD domain, G-beta repeat; similar to Coatomer alpha |
| repression | subunit (Alpha-coat protein) (Alpha-COP) (HEPCOP) (HEP-COP) (SP: P53621) [Homo sapiens] (68414.m06995) | ||||
| 71 | (A) Inorganic Nitrogen | D-D-D | 264260_at | At1g09210 | calreticulin 2 (CRT2) identical to SP|Q38858 Calreticulin 2 precursor {Arabidopsis thaliana} (68414.m01028) |
| repression | |||||
| 72 | (A) Inorganic Nitrogen | D-D-D | 264261_at | At1g09240 | nicotianamine synthase, putative similar to nicotianamine synthase [Lycopersicon esculentum][GI: 4753801], nicotianamine |
| repression | synthase 2 [Hordeum vulgare][GI: 4894912] (68414.m01031) | ||||
| 73 | (A) Inorganic Nitrogen | D-D-D | 263989_at | At2g42880 | mitogen-activated protein kinase, putative/MAPK, putative (MPK20) mitogen-activated protein kinase (MAPK)(AtMPK20), |
| repression | PMID: 12119167 (68415.m05309) | ||||
| 74 | (A) Inorganic Nitrogen | D-D-D | 263838_at | At2g36880 | S-adenosylmethionine synthetase, putative similar to S-adenosylmethionine synthetase 3 (Methionine adenosyltransferase 3. |
| repression | AdoMet synthetase 3) [Lycopersicon esculentum] SWISS-PROT: P43282 (68415.m04521) | ||||
| 75 | (A) Inorganic Nitrogen | D-D-D | 263836_at | At2g40330 | Bet v I allergen family protein contains Pfam profile PF00407: Pathogenesis-related protein Bet v I family (68415.m04972) |
| repression | |||||
| 76 | (A) Inorganic Nitrogen | D-D-D | 263662_at | At1g04430 | dehydration-responsive protein-related similar to early-responsive to dehydration stress ERD3 protein [Arabidopsis thaliana] |
| repression | GI: 15320410; contains Pfam profile PF03141: Putative methyltransferase (68414.m00434) | ||||
| 77 | (A) Inorganic Nitrogen | D-D-D | 263590_at | At2g01820 | leucine-rich repeat protein kinase, putative similar to protein kinase TMK1 gi|186888|gb|AAA32876; contains Pfam domains |
| repression | PF00560: Leucine Rich Repeat and PF00069: Protein kinase domain (68415.m00113) | ||||
| 78 | (A) Inorganic Nitrogen | D-D-D | 263326_at | At2g04280 | expressed protein (68415.m00420) |
| repression | |||||
| 79 | (A) Inorganic Nitrogen | D-D-D | 263153_s_at | At1g54010 | myrosinase-associated protein, putative similar to myrosinase-associated protein GI: 1769969 from [Brassica napus]; contains Pfam |
| repression | profile PF00657: Lipase/Acylhydrolase with GDSL-like motif (68414.m06153) | ||||
| 80 | (A) Inorganic Nitrogen | D-D-D | 262504_at | At1g21750 | protein disulfide isomerase, putative similar to SP|P29828 Protein disulfide isomerase precursor (PDI) (EC 5.3.4.1) {Medicago |
| repression | sativa}; isoform contains non-consensus GA donor splice site at intron 9 (68414.m02722) | ||||
| 81 | (A) Inorganic Nitrogen | D-D-D | 262373_at | At1g73120 | expressed protein (68414.m08454) |
| repression | |||||
| 82 | (A) Inorganic Nitrogen | D-D-D | 261728_at | At1g76160 | multi-copper oxidase type I family protein similar to pollen-specific BP10 protein [SP|Q00624][Brassica napus]; contains Pfam |
| repression | profile: PF00394 Multicopper oxidase (68414.m08844) | ||||
| 83 | (A) Inorganic Nitrogen | D-D-D | 261598_at | At1g49750 | leucine-rich repeat family protein contains leucine-rich repeats, Pfam: PF00560 (68414.m05579) |
| repression | |||||
| 84 | (A) Inorganic Nitrogen | D-D-D | 261198_at | At1g12940 | high-affinity nitrate transporter, putative similar to trans-membrane nitrate transporter protein AtNRT2:1 [Arabidopsis thaliana] |
| repression | GI: 3747058, high-affinity nitrate transporter ACH1 [Arabidopsis thaliana] GI: 3608362 (68414.m01503) | ||||
| 85 | (A) Inorganic Nitrogen | D-D-D | 261053_at | At1g01320 | tetratricopeptide repeat (TPR)-containing protein low similarity to SP|P46825 Kinesin light chain (KLC) {Loligo pealeii}; contains |
| repression | Pfam profile PF00515: TPR Domain (68414.m00048) | ||||
| 86 | (A) Inorganic Nitrogen | D-D-D | 261055_at | At1g01300 | aspartyl protease family protein contains Pfam domain, PF00026: eukaryotic aspartyl protease (68414.m00046) |
| repression | |||||
| 87 | (A) Inorganic Nitrogen | D-D-D | 260623_at | At1g08090 | high-affinity nitrate transporter (ACH1) identical to trans-membrane nitrate transporter protein AtNRT2:1 [Arabidopsis thaliana] |
| repression | GI: 3747058, high-affinity nitrate transporter ACH1 [Arabidopais thaliana] GI: 3608362 (68414.m00885) | ||||
| 88 | (A) Inorganic Nitrogen | D-D-D | 259969_at | At1g76550 | pyrophosphate-fructose-6-phosphate 1-phosphotransferase alpha subunit, putative/pyrophosphate-dependent 6- |
| repression | phosphofructose-1-kinase, putative strong similarity to SP|Q41140 Pyrophosphate-fructose 6-phosphate 1-phosphotransferase | ||||
| alpha subunit (EC 2.7.1.90) (PFP) (PPI-PFK) {Ricinus communis}; contains Pfam profile PF00365: Phosphofructokinase | |||||
| (68414.m08908) | |||||
| 89 | (A) Inorganic Nitrogen | D-D-D | 259813_at | At1g49860 | glutathione S-transferase, putative similar to GI: 860955 from [Hyoscyamus muticus] (Plant Physiol. 109 (1), 253-260 (1995)) |
| repression | (68414.m05590) | ||||
| 90 | (A) Inorganic Nitrogen | D-D-D | 259771_at | At1g29470 | dehydration-responsive protein-related similar to early-responsive to dehydration stress ERD3 protein [Arabidopsis thaliana] |
| repression | GI: 15320410; contains Pfam profile PF03141: Putative methyltransferase (68414.m03605) | ||||
| 91 | (A) Inorganic Nitrogen | D-D-D | 259786_at | At1g29660 | GDSL-motif lipase/hydrolase family protein low similarity to family II lipase EXL1 [Arabidopsis thaliana] GI: 15054382, contains |
| repression | InterPro Entry IPR001087 Lipolytic enzyme, G-D-S-L family (68414.m03625) | ||||
| 92 | (A) Inorganic Nitrogen | D-D-D | 259757_at | At1g77510 | protein disulfide isomerase, putative similar to protein disulfide isomerase precursor GB: P29828 GI: 4704786 [Medicago sativa]; |
| repression | Pfam HMM hit: PF00085 Thioredoxins (68414.m09026) | ||||
| 93 | (A) Inorganic Nitrogen | D-D-D | 259343_s_at | At3g03780 | 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase, putative/vitamin-B12-independent methionine synthase, |
| repression | putative/cobalamin-independent methionine synthase, putative very strong similarity to SP|O50008 5- | ||||
| methyltetrahydropteroyltriglutamate-homocysteine methyltransferase (EC 2.1.1.14) (Vitamin-B12-independent methionine | |||||
| synthase isozyme) (Cobalamin-independent methionine synthase isozyme) {Arabidopsis thaliana}; contains Pfam profile PF01717: | |||||
| Methionine synthase, vitamin-B12 independent (68416.m00386) | |||||
| 94 | (A) Inorganic Nitrogen | D-D-D | 259279_at | At3g01120 | cystathionine gamma-synthase, chloroplast/O-succinylhomoserine (Thiol)-lyase (CGS) identical to SP|P55217 Cystathionine |
| repression | gamma-synthase, chloroplast precursor (EC 4.2.99.9) (CGS) (O-succinylhomoserine (Thiol)-lyase) {Arabidopsis thaliana} | ||||
| (68418.m00016) | |||||
| 95 | (A) Inorganic Nitrogen | D-D-D | 259072_at | At3g11700 | beta-Ig-H3 domain-containing protein/fasciclin domain-containing protein contains Pfam profile PF02469: Fascictin domain |
| repression | (68416.m01434) | ||||
| 96 | (A) Inorganic Nitrogen | D-D-D | 259018_at | At3g07390 | auxin-responsive protein/auxin-induced protein (AIR12) identical (with 7 residue gap) to auxin-induced protein AIR12 GI: 11357190 |
| repression | [Arabidopsis thaliana] (68416.m00881) | ||||
| 97 | (A) Inorganic Nitrogen | D-D-D | 258415_at | At3g17390 | S-adenosylmethionine synthetase, putative similar to S-adenosylmethionine synthetase 2 (Methionine adenosyltransferase 2, |
| repression | AdoMet synthetase 2) [Catharanthus roseus] SWISS-PROT: Q96552 (68418.m02222) | ||||
| 98 | (A) Inorganic Nitrogen | D-D-D | 257173_at | At3g23810 | adenosylhomocysteinase, putative/S-adenosyl-L-homocysteine hydrolase, putative/AdoHcyase, putative strong similarity to |
| repression | SP|P50248|SAHH_TOBAC Adenosylhomocysteinase (EC 3.3.1.1) (S-adenosyl-L-homocysteine hydrolase) (AdoHcyase) {Nicotiana | ||||
| sylvestris}; contains Pfam profile PF00670: S-adenosyl-L-homocysteine hydrolase, NAD binding domain (68416.m02993) | |||||
| 99 | (A) Inorganic Nitrogen | D-D-D | 257071_at | At3g28180 | glycosyl transferase family 2 protein similar to beta-(1-3)-glucosyl transferase GB: AAC62210 GI: 3687658 from [Bradyrhizobium |
| repression | japonicum], cellulose synthase from Agrobacterium tumeficiens [gi: 710492] and Agrobacterium radiobacter [gi: 710493]; contains | ||||
| Pfam glycosyl transferase, group 2 family protein domain PF00535 (68416.m03521) | |||||
| 100 | (A) Inorganic Nitrogen | D-D-D | 256847_at | Atg13610 | oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to desacetoxyvindoline 4-hydroxylase [Catharanthus |
| repression | roseus][GI: 1916643], flavonol synthase 1 [SP|Q96330]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain | ||||
| (68416.m01713) | |||||
| 101 | (A) Inorganic Nitrogen | D-D-D | 256482_at | At1g31420 | leucine-rich repeat transmembrane protein kinase, putative contains Pfam profile: PF00069: Eukaryotic protein kinase domain |
| repression | (68414.m03848) | ||||
| 102 | (A) Inorganic Nitrogen | D-D-D | 256216_at | At1g56340 | calreticulin 1 (CRT1) identical to calreticulin (crt1) GI: 2052379 [Arabidopsis thaliana] (68414.m06476) |
| repression | |||||
| 103 | (A) Inorganic Nitrogen | D-D-D | 256224_at | At1g56330 | GTP-binding protein (SAR1B) identical to GTP-binding protein (SAR1B) [Arabidopsis thaliana] SP: Q01474 (68414.m06475) |
| repression | |||||
| 104 | (A) Inorganic Nitrogen | D-D-D | 255595_at | At4901700 | chitinase, putative similar to peanut type II chitinase GI: 1237025 from [Arachis hypogaea] (68417.m00221) |
| repression | |||||
| 105 | (A) Inorganic Nitrogen | D-D-D | 255474_at | At4g02470 | |
| repression | |||||
| 106 | (A) Inorganic Nitrogen | D-D-D | 255492_at | At4g02680 | tetratricopeptide repeat (TPR)-containing protein contains Pfam profile PF00515 TPR Domain (68417.m00363) |
| repression | |||||
| 107 | (A) Inorganic Nitrogen | D-D-D | 255068_at | At4g08920 | cryptochrome 1 apoprotein (CRY1)/flavin-type blue-light photoreceptor (HY4) contains Pfam PF03441: FAD binding domain of |
| repression | DNA photolyase; member of Pfam PF00875: deoxyribodipyrimidine photolyase superfamily; 99% identical to Cryptochrome 1 | ||||
| apoprotein (Blue light photoreceptor) (flavin-type blue-light photoreceptor) (SP: Q43125) [Arabidopsis thaliana] (68417.m01469) | |||||
| 108 | (A) Inorganic Nitrogen | D-D-D | 254925_at | At4g11380 | beta-adaptin, putative strong similarity to SP|Q10567 Adapter-related protein complex 1 beta 1 subunit (Beta-adaptin 1) [Homo |
| repression | sapiens], beta-adaptin [Drosophila melanogaster] GI: 434902; contains Pfam profile: PF01602 Adaptin N terminal region | ||||
| (68417.m01835) | |||||
| 109 | (A) Inorganic Nitrogen | D-D-D | 254805_at | At4g12480 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein identical to pEARLI 1 (Accession No. L43080): an |
| repression | Arabidopsis member of a conserved gene family (PGF95-099), Plant Physiol. 109 (4), 1497 (1995); contains Pfam protease | ||||
| inhibitor/seed storage/LTP family domain PF00234 (68417.m01973) | |||||
| 110 | (A) Inorganic Nitrogen | D-D-D | 254785-at | At4g12730 | fasciclin-like arabinogalactan-protein (FLA2) identical to gi_13377778_gb_AAK20858 (68417.m01999) |
| repression | |||||
| 111 | (A) Inorganic Nitrogen | D-D-D | 254740_s_at | At4g13890 | glycine hydroxymethyltransferase, putative/serine hydroxymethyltransferase, putative/serine/threonine aldolase, putative similar |
| repression | to serine hydroxymethyltransferase [Chlamydomonas reinhardtii] GI: 17066746; contains Pfam profile PF00484: serine | ||||
| hydroxymethyltransferase (68417.m02152) | |||||
| 112 | (A) Inorganic Nitrogen | D-D-D | 254564_at | At4g19170 | 9-cis-epoxycarotenoid dioxygenase, putative/neoxanthin cleavage enzyme, putative/carotenoid cleavage dioxygenase, putative |
| repression | similar to 9-cis-epoxycarotenoid dioxygenase [Phaseolus vulgaris][GI: 6715257]: neoxanthin cleavage enzyme, Lycopersicon | ||||
| esculentum, PATX: E325797 (68417.m02829) | |||||
| 113 | (A) Inorganic Nitrogen | D-D-D | 254166_at | At4g24190 | shepherd protein (SHD)/clavata formation protein, putative nearly identical to SHEPHERD [Arabidopsis thaliana] GI: 19570872; |
| repression | contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 | ||||
| protein (68417.m03472) | |||||
| 114 | (A) Inorganic Nitrogen | D-D-D | 253887_at | At4g27730 | oligopeptide transporter OPT family protein similar to SP|P40900 Sexual differentiation process protein isp4 {Schizosaccharomyces |
| repression | pombe}, oligopeptide transporter Opt1p [Candida albicans] GI: 2367386; contains Pfam profile PF03169: OPT oligopeptide | ||||
| transporter protein (68417.m03985) | |||||
| 115 | (A) Inorganic Nitrogen | D-D-D | 253609_at | At4g30190 | ATPase 2, plasma membrane-type, putative/proton pump 2, putative/proton-exporting ATPase, putative strong similarity to |
| repression | SP|P19456 ATPase 2, plasma membrane-type (EC 3.6.3.6) (Proton pump 2) {Arabidopsis thaliana}; contains InterPro accession | ||||
| IPR001757: ATPase, E1-E2 type; contains Pfam profile PF00690: Cation transporter/ATPase, N-terminus (68417.m04292) | |||||
| 116 | (A) Inorganic Nitrogen | D-D-D | 253534_at | At4g31500 | cytochrome P450 83B1 (CYP83B1) Identical to Cytochrome P450 (SP: O65782)[Arabidopsis thaliana] (68417.m04474) |
| repression | |||||
| 117 | (A) Inorganic Nitrogen | D-D-D | 253050_at | At4g37450 | arabinogalactan-protein (AGP18) identical to gi_11935088_gb_AAG41964 (68417.m05301) |
| repression | |||||
| 118 | (A) Inorganic Nitrogen | D-D-D | 252971_at | At4g38770 | proline-rich family protein (PRP4) similar to proline-rich protein [Arabidopsis thaliana] gi|6782442|gb|AAF28388; contains proline- |
| repression | rich extensin domains, INTERPRO: IPR002965 (68417.m05490) | ||||
| 119 | (A) Inorganic Nitrogen | D-D-D | 252831_at | At4g39980 | 2-dehydro-3-deoxyphosphoheptonate aldolase 1/3-deoxy-D-arabino-heptulosonate 7-phosphate synthase 1/DAHP synthetase 1 |
| repression | (DHS1) nearly identical to SP|P29965 (68417.m05662) | ||||
| 120 | (A) Inorganic Nitrogen | D-D-D | 252255_at | At3949220 | pectinesterase family protein contains Pfam profile: PF01095 pectinesterase (68416.m05379) |
| repression | |||||
| 121 | (A) Inorganic Nitrogen | D-D-D | 251918_at | At3g54040 | photoassimilate-responsive protein-related contains weak similarity to mRNA inducible by sucrose and salicylic acid expressed in |
| repression | sugar-accumulating tobacco plants (GI: 871487) [Nicotiana tabacum] (68416.m05975) | ||||
| 122 | (A) Inorganic Nitrogen | D-D-D | 251899_at | At3g54400 | aspartyl protease family protein contains Pfam profile: PF00026 eukaryotic aspartyl protease (68416.m06015) |
| repression | |||||
| 123 | (A) Inorganic Nitrogen | D-D-D | 251847_at | At3g54640 | tryptophan synthase, alpha subunit (TSA1) identical to gi: 619753 (68416.m06045) |
| repression | |||||
| 124 | (A) Inorganic Nitrogen | D-D-D | 251413_at | At3g60320 | expressed protein contains Pfam profiles: PF04782: protein of unknown function (DUF632), PF04783: protein of unknown function |
| repression | (DUF630) (68416.m06742) | ||||
| 125 | (A) Inorganic Nitrogen | D-D-D | 251287_at | At3g61820 | aspartyl protease family protein contains Pfam domain, PF00026: eukaryotic aspartyl protease (68416.m06939) |
| repression | |||||
| 126 | (A) Inorganic Nitrogen | D-D-D | 251182_at | At3g62800 | DNAJ heat shock family protein similar to DnaJ homolog subfamily B member 11 precursor (SP: Q99KV1)[Mus musculus]; contains |
| repression | Pfam PF00226: DnaJ domain; contains PfaPF01556: DnaJ C terminal regionm (68416.m07032) | ||||
| 127 | (A) Inorganic Nitrogen | D-D-D | 251174_at | At3g63200 | patatin-related low similarity to patatin-like latex protein allergen Hev b 7 - Hevea brasiliensis, EMBL: AF113546; contains patatin |
| repression | domain PF01734 (68416.m07100) | ||||
| 128 | (A) Inorganic Nitrogen | D-D-D | 251109_at | At5g01600 | ferritin 1 (FER1) identical to ferritin [Arabidopsis thaliana] GI: 1246401, GI: 8163920 (68418.m00075) |
| repression | |||||
| 129 | (A) Inorganic Nitrogen | D-D-D | 250437_at | At5g10430 | arabinogalactan-protein (AGP4) identical to gi_3883126_gb_AAC77826 (68418.m01209) |
| repression | |||||
| 130 | (A) Inorganic Nitrogen | D-D-D | 250207_at | At5g13930 | chalcone synthase/naringenin-chalcone synthase identical to SP|P13114 (68418.m01629) |
| repression | |||||
| 131 | (A) Inorganic Nitrogen | D-D-D | 250100_at | At5g16570 | glutamine synthetase, putative similar to glutamine synthetase, cytosolic isozyme (glutamate-ammonia lipase) [Alfalfa] SWISS- |
| repression | PROT: P04078 (68418.m01939) | ||||
| 132 | (A) Inorganic Nitrogen | D-D-D | 249867_at | At5g23020 | 2-isopropylmalate synthase 2 (IMS2) identical to 2-isopropylmalate synthase (IMS2) [Arabidopsis thaliana] GI: 12330689 |
| repression | (68416.m02691) | ||||
| 133 | (A) Inorganic Nitrogen | D-D-D | 249812_at | At5g23830 | MD-2-related lipid recognition domain-containing protein/ML domain-containing protein contains Pfam profile PF02221: ML |
| repression | domain (68418.m02797) | ||||
| 134 | (A) Inorganic Nitrogen | D-D-D | 249677_at | At5g35970 | DNA-binding protein, putative similar to SWISS-PROT: Q60560 DNA-binding protein SMUBP-2 (Immunoglobulin MU binding |
| repression | protein 2, SMUBP-2) [Mesocricetus auratus] (68418.m04332) | ||||
| 135 | (A) Inorganic Nitrogen | D-D-D | 249581_at | At5g37600 | glutamine synthetase, putative similar to glutamine synthetase, cytosolic isozyme (Glutamate-ammonia ligase, GS1) [Lotus |
| repression | japonicus] SWISS-PROT: Q42899 (68418.m04529) | ||||
| 136 | (A) Inorganic Nitrogen | D-D-D | 249037_at | At5g44130 | fasciclin-like arabinogalactan-protein, putative similar to gi_13377784_gb_AAK20861 (68418.m05401) |
| repression | |||||
| 137 | (A) Inorganic Nitrogen | D-D-D | 248942_at | At5g45480 | expressed protein contains Pfam domain, PF04578: Protein of unknown function, DUF594 (68418.m05587) |
| repression | |||||
| 138 | (A) Inorganic Nitrogen | D-D-D | 248721_at | At5g47780 | glycosyl transferase family 8 protein contains Pfam profile: PF01501 glycosyl transferase family 8; strong similarity to unknown |
| repression | protein (emb|CAB71043.1) (68418.m05902) | ||||
| 139 | (A) Inorganic Nitrogen | D-D-D | 248703_at | At5g48430 | expressed protein low similarity to extracellular dermal glycoprotein EDGP precursor [Daucus carota] GI: 285741, SP|P13917 Basic |
| repression | 7S globulin precursor {Glycine max}; expression supported by MPSS (68418.m05988) | ||||
| 140 | (A) Inorganic Nitrogen | D-D-D | 248252_at | At5g53250 | arabinogalactan-protein, putative (AGP22) similar to arabinogalactan protein [Arabidopsis thaliana] gi|10880509|gb|AAG24284 |
| repression | (68418.m06819) | ||||
| 141 | (A) Inorganic Nitrogen | D-D-D | 248118_at | At5g55050 | GDSL-motif lipase/hydrolase family protein similar to family II lipases EXL3 GI: 15054386, EXL1 GI: 15054382, EXL2 GI: 15054384 |
| repression | from [Arabidopsis thaliana]; contains Pfam profile PF00657: GDSL-like Lipase/Acylhydrolase (68418.m06861) | ||||
| 142 | (A) Inorganic Nitrogen | D-D-D | 248037_at | At5g55930 | oligopeptide transporter OPT family protein similar to SP|P40900 Sexual differentiation process protein isp4 {Schizosaccharomyces |
| repression | pombe}, oligopeptide transporter Opt1p [Candida albicans] GI: 2367386; contains Pfam profile PF03169: OPT oligopeptide | ||||
| transporter protein (68418.m06976) | |||||
| 143 | (A) Inorganic Nitrogen | D-D-D | 247517_at | At5g61780 | tudor domain-containing protein/nuclease family protein contains Pfam domains PF00567: Tudor domain and PF00565: |
| repression | Staphylococcal nuclease homologue (68418.m07753) | ||||
| 144 | (A) Inorganic Nitrogen | D-D-D | 247492_at | At5g61890 | AP2 domain-containing transcription factor family protein similar to RAP2.6 (GP: 17065542) {Arabidopsis thaliana}; AP2 domain |
| repression | containing protein, Arabidopsis thaliana, EMBL: ATAF3099 (68418.m07764) | ||||
| 145 | (A) Inorganic Nitrogen | D-D-D | 247494_at | At5g61790 | calnexin 1 (CNX1) identical to calnexin homolog 1, Arabidopsis thaliana, EMBL: AT08315 [SP|P29402] (68418.m07754) |
| repression | |||||
| 146 | (A) Inorganic Nitrogen | D-D-D | 247480_at | At5g62390 | calmodulin-binding family protein contains IQ calmodulin-binding motif, Pfam: PF00612 (68418.m07830) |
| repression | |||||
| 147 | (A) Inorganic Nitrogen | D-D-D | 247189_at | At5g65390 | arabinogalacten-protein (AGP7) (68418: m08224) |
| repression | |||||
| 148 | (A) Inorganic Nitrogen | D-D-D | 246935_at | At5g25350 | F-box family protein contains Pfam PF00646: F-box domain and Pfam PF00560: Leucine Rich Repeat (6 copies); similar to F-box |
| repression | protein FBL6 (GI: 4432860) [Homo sapiens] (68418.m03007) | ||||
| 149 | (A) Inorganic Nitrogen | D-D-D | 246855_at | At5g26280 | meprin and TRAF homology domain-containing protein/MATH domain-containing protein low similarity to ubiquitin-specific |
| repression | protease 12 [Arabidopsis thaliana] GI: 11993471; contains Pfam profile PF00917: MATH domain (68418.m03137) | ||||
| 150 | (A) Inorganic Nitrogen | D-D-D | 246510_at | At5g15410 | cyclic nucleotide-regulated ion channel/cyclic nucleotide-gated channel (CNGC2) identical to cyclic nucleotide-gated cation |
| repression | channel GI: 3894399 from [Arabidopsis thaliana] (68418.m01803) | ||||
| 151 | (A) Inorganic Nitrogen | D-D-D | 245956_s_at | At5g28540 | luminal binding protein 1 (BiP-1) (BP1) SWISS-PROT: Q9LKR3 PMID: 8888624 (68418.m03480) |
| repression | |||||
| 152 | (A) Inorganic Nitrogen | D-D-D | 245915_s_at | At5g19770 | tubulin alpha-3/alpha-5 chain (TUA3) nearly identical to SP|P20383 Tubulin alpha-3/alpha-5 chain {Arabidopsis thaliana} |
| repression | (68418.m02350) | ||||
| 153 | (A) Inorganic Nitrogen | D-D-D | 245874_at | At1g26250 | proline-rich extensin, putative similar to extensin gi|1165322|gb|AAB53156; contains proline-rich extensin domains, |
| repression | INTERPRO: IPR002965 (68414.m03202) | ||||
| 154 | (A) Inorganic Nitrogen | D-D-D | 245688_at | At1g28290 | pollen Ole e 1 allergen and extensin family protein similar to arabinogalactan protein [Daucus carota] GI: 11322245; contains Pfam |
| repression | profile PF01190: Pollen proteins Ole e I family (68414.m03472) | ||||
| 155 | (A) Inorganic Nitrogen | D-D-D | 245281_at | At4g15560 | 1-deoxy-D-xylulose 5-phosphate synthase, putative/1-deoxyxylulose-5-phosphate synthase, putative/DXP-synthase, putative |
| repression | (DEF) (CLA1) identical to SP|Q38854 Probable 1-deoxy-D-xylulose 5-phosphate synthase, chloroplast precursor (EC 4.1.3.37) (1- | ||||
| deoxyxylulose-5-phosphate synthase) (DXP synthase) (DXPS). [Mouse-ear cress] {Arabidopsis thaliana}, DEF (deficient in | |||||
| photosynthesis) protein [Arabidopsis thaliana] GI: 1399261 (68417.m02377) | |||||
| 156 | (A) Inorganic Nitrogen | D-D-D | 245293_at | At4g16660 | heat shock protein 70, putative/HSP70, putative (68417.m02517) |
| repression | |||||
| 157 | (A) Inorganic Nitrogen | D-D-D | 245175_at | At2g47470 | thioredoxin family protein similar to protein disulfide isomerase [Dictyostelium discoideum] GI: 2627440; contains Pfam profile: |
| repression | PF00085 Thioredoxin (68415.m05924) | ||||
| 158 | (A) Inorganic Nitrogen | D-D-D | 245012_at | ndhC | NADH dehydrogenase D3 |
| repression | |||||
| 159 | (A) Inorganic Nitrogen | D-D-D | 244984_at | rps3 chloropl | ribosomal protein S3 |
| repression | |||||
| 1 | (B) Inorganic Nitrogen | I-I-NC | 266296_at | At2g29420 | glutathione S-transferase, putative (68415.m03575) |
| induction and external | |||||
| 2 | (B) Inorganic Nitrogen | I-I-NC | 265499_at | At2g15480 | UDP-glucoronosyl/UDP-glucosyl transferase family protein contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl |
| induction and external | transferase (68415.m01771) | ||||
| 3 | (B) Inorganic Nitrogen | I-I-NC | 263046_at | At2g05380 | glycine-rich protein (GRP3S) identical to cDNA glycine-rich protein 3 short isoform (GRP3S) GI: 4206766 (68415.M00566) |
| induction and external | |||||
| 4 | (B) Inorganic Nitrogen | I-I-NC | 262119_s_at | At1g02930 | glutathione S-transferase, putative similar to glutathione S-transferase GI: 860955 from [Hyoscyamus muticus] (68414.m00260) |
| induction and external | |||||
| 5 | (B) Inorganic Nitrogen | I-I-NC | 260602_at | At1g55920 | serine O-acetyltransferase, putative identical to GI: 608677 from [Arabidopsis thaliana] (68414.m06414) |
| induction and external | |||||
| 6 | (B) Inorganic Nitrogen | I-I-NC | 259875_s_at | At1g76690 | 12-oxophytodienoate reductase (OPR2) identical to 12-oxophytodienoate reductase OPR2 GB: AAC78441 [Arabidopsis thaliana] |
| induction and external | (68414.m08924) | ||||
| 7 | (B) Inorganic Nitrogen | I-I-NC | 259428_at | At1g01560 | mitogen-activated protein kinase, putative/MAPK, putative (MPK11) similar to MAP kinase 5 GI: 4239889 from [Zea mays]; mitogen |
| induction and external | activated protein kinase (MAPK)(AtMPK11), PMID: 12119167 (68414.m00073) | ||||
| 8 | (B) Inorganic Nitrogen | I-I-NC | 256245_at | At3g12580 | heat shock protein 70, putative/HSP70, putative strong similarity to heat shock protein GI: 425194 [Spinacia olerecea] |
| induction and external | (68416.m01567) | ||||
| 9 | (B) Inorganic Nitrogen | I-I-NC | 254256_at | At4g23180 | receptor-like protein kinase 4, putative (RLK4) nearly identical to receptor-like protein kinase 4 [Arabidopsis thaliana] GI: 13506745; |
| induction and external | contains Pfam domain, PF00069: Protein kinase domain; identical to cDNA receptor-like protein kinase 4 (RLK4) GI: 13506744 | ||||
| (68417.m03344) | |||||
| 10 | (B) Inorganic Nitrogen | I-I-NC | 253268_s_at | At4g34135 | UDP-glucoronosyl/UDP-glucosyl transferase family protein contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl |
| induction and external | transferase (68417.m04842) | ||||
| 11 | (B) Inorganic Nitrogen | I-I-NC | 252037_at | At3g51920 | calmodulin-9 (CAM9) identical to calmodulin 9 GI: 5825602 from [Arabidopsis thaliana]; contains Pfam profile PF00036: EF hand |
| induction and external | (68416.m05695) | ||||
| 12 | (B) Inorganic Nitrogen | I-I-NC | 248657_at | At5g48570 | peptidyl-prolyl cis-trans isomerase, putative/FK506-binding protein, putative similar to rof1 [Arabidopsis thaliana] GI: 1373396 |
| induction and external | (68418.m06007) | ||||
| 13 | (B) Inorganic Nitrogen | I-I-NC | 247218_at | At5g65010 | asparagine synthetase 2 (ASN2) identical to asparagine synthetase (ASN2) [Arabidopsis thaliana] GI: 3859536 (68418.m08177) |
| induction and external | |||||
| 14 | (B) Inorganic Nitrogen | I-I-NC | 245252_at | At4g17500 | ethylene-responsive element-binding protein 1 (ERF1)/EREBP-2 protein identical to SP|O80337 Ethylene responsive element |
| induction and external | binding factor 1 (EREBP-2 protein) [Arabidopsis thaliana]; a false single by exon was added to circumvent a single basepair | ||||
| insertion in the genomic sequence, supported by cDNA/genome alignment. (68417.m02618) | |||||
| 15 | (B) Inorganic Nitrogen | I-I-NC | 245011_at | psbG | photosystem II G protein |
| induction and external | |||||
| 16 | (B) Inorganic Nitrogen | D-D-I | 267147_at | At2g38240 | oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to flavonol synthase [Citrus unshiu][gi: 4126403], leucoanthocyanidin |
| repression and extern | dioxygenase [Daucus carota][gi: 5924383]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain (68415.m04696) | ||||
| 17 | (B) Inorganic Nitrogen | D-D-I | 263497_at | At2g42540 | cold-responsive protein/cold-regulated protein (cor15a) identical to cold-regulated protein cor15a [Arabidopsis thaliana] |
| repression and extern | GI: 507149; contains Pfam profile PF02987: Late embryogenesis abundant protein (68415.m05264) | ||||
| 18 | (B) Inorganic Nitrogen | D-D-I | 251770_at | At3g55970 | oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to leucoanthocyanidin dioxygenase, Malus domestics, SP|P51091; |
| repression and extern | contains Pfam profile PF03171: oxidoreductase, 2OG-Fe(II) oxygenase family (68416.m06219) | ||||
| 19 | (B) Inorganic Nitrogen | D-D-NC | 266743_at | At2g02990 | ribonuclease 1 (RNS1) identical to ribonuclease SP: P42813 Ribonuclease 1 precursor (EC 3.1.27.1) {Arabidopsis thaliana} |
| repression and extern | GI: 561998 from [Arabidopsis thaliana] (68415.m00251) | ||||
| 20 | (B) Inorganic Nitrogen | D-D-NC | 266225_at | At2g28900 | mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein contains Pfam PF02466: |
| repression and extern | Mitochondrial import inner membrane translocase subunit Tim17 (68415.m03512) | ||||
| 21 | (13) Inorganic Nitrogen | D-D-NC | 265411_at | At2g16630 | proline-rich family protein contains proline-rich extensin domains, INTERPRO: IPR002965 (68415.m01909) |
| repression and extern | |||||
| 22 | (B) Inorganic Nitrogen | D-D-NC | 265414_at | At2g16660 | nodulin family protein similar to nodulin-like protein [Arabidopsis thaliana] GI: 3329368, nodule-specific protein Nlj70 [Lotus |
| repression and extern | japonicus] GI: 3329366 (68415.m01912) | ||||
| 23 | (B) Inorganic Nitrogen | D-D-NC | 264611_at | At1g04680 | pectate lyase family protein similar to pectate lyase GP: 14531296 from [Fregaria x ananassa] (68414.m00465) |
| repression and extern | |||||
| 24 | (B) Inorganic Nitrogen | D-D-NC | 262784_at | At1g10760 | starch excess protein (SEX1) identical to SEX1 [Arabidopsis thaliana] GI: 12044358: supporting cDNA |
| repression and extern | gi|12044357|gb|AF312027.1|AF312027 (68414.m01231) | ||||
| 25 | (B) Inorganic Nitrogen | D-D-NC | 262068_at | At1g80070 | splicing factor, putative strong similarity to splicing factor Prp8 [Homo sapiens] GI: 3661610; contains Pfam profile PF01398: |
| repression and extern | Mov34/MPN/PAD-1 family (68414.m09373) | ||||
| 28 | (B) Inorganic Nitrogen | D-D-NC | 261485_at | At1g14360 | UDP-galactose/UDP-glucose transporter, putative very similar to UDP-galactose/UDP-glucose transporter (GI: 22651763) |
| repression and extern | {Arabidopsis thaliana} (68414.m01702) | ||||
| 27 | (B) Inorganic Nitrogen | D-D-NC | 259936_at | At1g71220 | UDP-glucose:glycoprotein glucosyltransferase, putative similar to UDP-glucose:glycoprotein glucosyltransferase precursor |
| repression and extern | GB: Q09332 [SP|Q09332] from Drosophila melanogaster, [gi: 7670748] and [gi: 11346464] from Homo sapiens (68414.m08219) | ||||
| 28 | (B) Inorganic Nitrogen | D-D-NC | 259789_at | At1g29395 | stress-responsive protein, putative similar to cold acclimation WCOR413-like protein gamma form [Hordeum vulgare] |
| repression and extern | gi|18449100|gb|AAL69988; similar to stress-regulated protein SAP1 [Xerophyta viscose] gi|21360378|gb|AAM47505 | ||||
| (68414.m03595) | |||||
| 29 | (B) Inorganic Nitrogen | D-D-NC | 259640_at | At1g52400 | glycosyl hydrolase family 1 protein/beta-glucosidase, putative (BG1) contains Pfam PF00232: Glycosyl hydrolase family 1 |
| repression and extern | domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase; identical to GI: 6651430 from [Arabidopsis thaliana] | ||||
| (68414.m05913) | |||||
| 30 | (B) Inorganic Nitrogen | D-D-NC | 259553_x_at | At1g21310 | proline-rich extensin-like family protein contains extensin-like region, Pfam: PF04554 (68414.m02662) |
| repression and extern | |||||
| 31 | (B) Inorganic Nitrogen | D-D-NC | 258040_at | At3g21190 | expressed protein contains Pfam PF03138: Plant protein family. The function of this family of plant proteins is unknown; |
| repression and extern | (68416.m02678) | ||||
| 32 | (B) Inorganic Nitrogen | D-D-NC | 257202_at | At3g23750 | leucine-rich repeat family protein/protein kinase family protein contains Pfam domains PF00560: Leucine Rich Repeat and |
| repression and extern | PF00069: Protein kinase domain (68416.m02986) | ||||
| 33 | (B) Inorganic Nitrogen | D-D-NC | 256787_at | At3g13790 | beta-fructosidase (BFRUCT1)/beta-fructofuranosidase/cell wall invertase identical to beta-fructofuranosidase GI: 402740 from |
| repression and extern | [Arabidopsis thaliana] (68416.m01742) | ||||
| 34 | (B) Inorganic Nitrogen | D-D-NC | 255942_at | At1g22360 | UDP-glucoronosyl/UDP-glucosyl transferase family protein contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl |
| repression and extern | transferase (68414.m02797) | ||||
| 35 | (B) Inorganic Nitrogen | D-D-NC | 254832_at | At4g12490 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein similar to pEARLI 1 (Accession No. L43080): an |
| repression and extern | Arabidopsis member of a conserved gene family (PGF95-099), Plant Physiol 109 (4), 1497 (1995); contains Pfam protease | ||||
| inhibitor/seed storage/LTP family domain PF00234 (88417.m01974) | |||||
| 36 | (B) Inorganic Nitrogen | D-D-NC | 254468_at | At4g20460 | NAD-dependent epimerase/dehydratase family protein similar to UDP-galactose 4-epimerase from Cyamopsis tetragonoloba |
| repression and extern | GI: 3021357 [EMBL: AJ005082], Bacillus subtilis SP|P55180; contains Pfam profile PF01370 NAD dependent | ||||
| epimerase/dehydratase family (68417.m02985) | |||||
| 37 | (B) Inorganic Nitrogen | D-D-NC | 254361_at | At4g22212 | expressed protein (68417.m03211) |
| repression and extern | |||||
| 38 | (B) Inorganic Nitrogen | D-D-NC | 252618_at | At3g45140 | lipoxygenase (LOX2) identical to SP|P38418 (68416.m04872) |
| repression and extern | |||||
| 39 | (B) Inorganic Nitrogen | D-D-NC | 252066_at | At3g51550 | protein kinase family protein contains protein kinase domain, Pfam: PF00069 (68416.m05645) |
| repression and extern | |||||
| 40 | (B) Inorganic Nitrogen | D-D-NC | 251513_at | At3g59220 | pirin, putative similar to SP|O00625 Pirin {Homo sapiens}; contains Pfam profile PF02678: Pirin (68416.m06602) |
| repression and extern | |||||
| 41 | (B) Inorganic Nitrogen | D-D-NC | 251390_at | At3g60860 | guanine nucleotide exchange family protein similar to guanine nucleotide exchange factor, Homo sapiens, GI: 5456754; contains |
| repression and extern | Pfam profile PF01369: Sec7 domain (68416.m06808) | ||||
| 42 | (B) Inorganic Nitrogen | D-D-NC | 250930_at | At5g03160 | DNAJ heat shock N-terminal domain-containing protein similar to P58 protein. Bos primigenius taurus, PIR: A56534; similar to p58 |
| repression and extern | (GI: 1353270) {Homo sapiens}; contains Pfam PF00226: DnaJ domain; contains Pfam PF00515: TPR Domain (68418.m00264) | ||||
| 43 | (B) Inorganic Nitrogen | D-D-NC | 250690_at | At5g06530 | ABC transporter family protein (68418.m00736) |
| repression and extern | |||||
| 44 | (B) Inorganic Nitrogen | D-D-NC | 250661_at | At5g07030 | aspartyl protease family protein contains Pfam profile: PF00026 eukaryotic aspartyl protease (68418.m00796) |
| repression and extern | |||||
| 45 | (B) Inorganic Nitrogen | D-D-NC | 250662_at | At5g07010 | sulfotransferase family protein similar to steroid sulfotransferase 3 [Brassica napus] GI: 3420008, steroid sulfotransferase 1 |
| repression and extern | [Brassica napus] GI: 3420004; contains Pfam profile PF00685: Sulfotransferase domain (68418.m00794) | ||||
| 46 | (B) Inorganic Nitrogen | D-D-NC | 247552_at | At5g60920 | phytochetatin synthetase. putative/COBRA cell expansion protein COB, putative similar to phytochelatin synthetase GI: 29570314; |
| repression and extern | similar to GB: AAK56072; identified in Roudier, et al, Plant Phys. (2002) 130: 538-548 (PMID: 12376623); identical to cDNA putative | ||||
| phytochelatin synthetase GI: 3559804, contains Pfam profile PF04833: Phytochelatin synthetase-like conserved region | |||||
| (68418.m07642) | |||||
| 47 | (B) Inorganic Nitrogen | D-D-NC | 247284_at | At5g64410 | oligopeptide transporter OPT family protein similar to SP|P40900 Sexual differentiation process protein isp4 {Schizosaccharomyces |
| repression and extern | pombe}; contains Pfam profile PF03169: OPT oligopeptide transporter protein (68418.m08091) | ||||
| 48 | (B) Inorganic Nitrogen | D-D-NC | 247251_at | At5g64740 | cellulose synthase, catalytic subunit, putative similar to gi: 2827141 cellulose synthase catalytic subunit (Ath-A), Arabidopsis |
| repression and extern | thaliana (68418.m08141) | ||||
| 1 | (C) Internal Glu induction | I-NC-NC | 267366_at | At2g44310 | calcium-binding EF hand family protein contains INTERPRO: IPR002048 calcium-binding EF-hand domain (68415.m05513) |
| 2 | (C) Internal Glu induction | I-NC-NC | 266983_at | At2g39400 | hydrolase, alpha/beta fold family protein similar to monoglyceride lipase from [Homo sapiens] GI: 14594904, [Mus musculus] |
| GI: 2632162; contains Pfam profile PF00561: hydrolase, alpha/beta fold family (68415.m04835) | |||||
| 3 | (C) Internal Glu induction | I-NC-NC | 266290_at | At2929490 | glutathione S-transferase, putative similar to glutathione S-transferase 103-1A [Arabidopsis thaliana] SWISS-PROT: P46421 |
| (68416.m03582) | |||||
| 4 | (C) Internal Glu induction | I-NC-NC | 265662_at | At2g24500 | zinc finger (C2H2 type) family protein contains Pfam profile: PF00098 zinc finger, C2H2 type (68415.m02927) |
| 5 | (C) Internal Glu induction | I-NC-NC | 265116_at | At1g62480 | vacuolar calcium-binding protein-related contains weak similarity to vacuolar calcium binding protein [Raphanus sativus] |
| gi|9049359|dbj|BAA99394 (68414.m07050) | |||||
| 6 | (C) Internal Glu induction | I-NC-NC | 264561_at | At1g55810 | uracil phosphoribosyltransferase, putative/UMP pyrophosphorylase, putative/UPRTase, putative similar to SP|O65583 Uracil |
| phosphoribosyltransferase (EC 2.4.2.9) (UMP pyrophosphorylase) (UPRTase) {Arabidopsis thaliana}; contains Pfam profile | |||||
| PF00485: Phosphoribulokinase/Uridine kinase family (68414.m06394) | |||||
| 7 | (C) Internal Glu induction | I-NC-NC | 264279_s_at | At1g78820 | curculin-like (mannose-binding) lectin family protein/PAN domain-containing protein similar to S locus glycoprotein [Brassica rapa] |
| GI: 12246840; contains Pfam profile PF01453: Lectin (probable mannose binding) (68414.m09188) | |||||
| 8 | (C) Internal Glu induction | I-NC-NC | 263866_at | At2g36950 | heavy-metal-associated domain-containing protein nearly identical to famesylated protein ATFP2 [GI: 4097545] Pfam profile |
| PF00403: Heavy-metal-associated domain (68415.m04531) | |||||
| 9 | (C) Internal Glu induction | I-NC-NC | 263403_at | At2g04040 | MATE efflux family protein contains Pfam profile: PF01554 uncharacterized membrane protein family (68415.m00385) |
| 10 | (C) Internal Glu induction | I-NC-NC | 263275_at | At2g14170 | methylmalonate-semialdehyde dehydrogenase, putative similar to methylmalonate-semialdehyde dehydrogenase [acylating], |
| mitodiondrial precursor (MMSDH) [Rattus norvegicus] SWISS-PROT: Q02253 (68415.m01578) | |||||
| 11 | (C) Internal Glu induction | I-NC-NC | 263118_at | At1g03090 | methylcrotonyl-CoA carboxylase alpha chain, mitochondrial/3-methylcrotonyl-CoA carboxylase 1 (MCCA) nearly identical to |
| SP|Q42523 Methylcrotonyl-CoA carboxylase alpha chain, mitochondrial precursor (EC 6.4.1.4) (3-Methylcrotonyl-CoA carboxylase | |||||
| 1) (MCCase alpha subunit) (3-methylcrotonyl-CoA:carbon dioxide ligase alpha subunit) {Arabidopsis thaliana} (68414.m00283) | |||||
| 12 | (C) Internal Glu induction | I-NC-NC | 262603_at | At1g15380 | lactoylglutathione lyase family protein/glyoxalase I family protein contains glyoxalase family protein domain, Pfam: PF00903 |
| (68414.m01841) | |||||
| 13 | (C) Internal Glu induction | I-NC-NC | 262517_at | At1g17180 | glutathione S-transferase, putative Second of three repeated putative glutathione transferases. 72% identical to glutathione |
| transferase [Arabidopsis thaliana] (gi|4006934). Location of ests 191A10T7 (gb|R90188) and 171N13T7 (gb|R65532) | |||||
| (68414.m02094) | |||||
| 14 | (C) Internal Glu induction | I-NC-NC | 262518_at | At1g17170 | glutathione S-transferase, putative One of three repeated putative glutathione transferases. 72% identical to glutathione |
| transferase [Arabidopsis thaliana] (gi|4006934) (68414.m02093) | |||||
| 15 | (C) Internal Glu induction | I-NC-NC | 262049_at | At1g80180 | expressed protein (68414.m09384) |
| 16 | (C) Internal Glu induction | I-NC-NC | 260592_at | At1g55850 | cellulose synthase family protein similar to cellulose synthase catalytic subunit [gi: 13925881] from Nicotiana alata, cellulose |
| synthase-5 [gi: 9622882] from Zea mays (68414.m06405) | |||||
| 17 | (C) Internal Glu induction | I-NC-NC | 259996_at | At1g67910 | expressed protein (68414.m07755) |
| 18 | (C) Internal Glu induction | I-NC-NC | 259911_at | At1g72680 | cinnamyl-alcohol dehydrogenase, putative similar to cinnamyl-alcohol dehydrogenase GB: AAC35846 [Medicago sativa], |
| SP|Q08350 [Picea abies] (68414.m08405) | |||||
| 19 | (C) Internal Glu induction | I-NC-NC | 259478_at | At1g18980 | germin-like protein, putative similar to germin-like protein subfamily T member 1 [SP|P92995]; contains PS00725 germin family |
| signature (68414.m02361) | |||||
| 20 | (C) Internal Glu induction | I-NC-NC | 258837_at | At3g07110 | 60S ribosomal protein L13A (RPL13aA) similar to ribosomal protein L13A GB: O49885 [Lupinus luteus] (68416.m00847) |
| 21 | (C) Internal Glu induction | I-NC-NC | 258699_at | At3g09460 | |
| 22 | (C) Internal Glu induction | I-NC-NC | 258284_at | At3g16080 | 60S ribosomal protein L37 (RPL37C) similar to ribosomal protein L37 GB: BAA04888 from [Homo sapiens] (68416.m02032) |
| 23 | (C) Internal Glu induction | I-NC-NC | 257658_at | At3g13230 | expressed protein (68416.m01665) |
| 24 | (C) Internal Glu induction | I-NC-NC | 256965_at | At3g13450 | 2-oxoisovalerate dehydrogenase/3-methyl-2-oxobutanoate dehydrogenase/branched-chain alpha-keto acid dehydrogenase E1 |
| beta subunit (DIN4) identical to branched chain alpha-keto acid dehydrogenase E1 beta subunit [Arabidopsis thaliana] GI: 7021286 | |||||
| (68416.m01692) | |||||
| 25 | (C) Internal Glu induction | I-NC-NC | 256453_at | At1g75270 | dehydroascorbate reductase, putative similar to GI: 6939839 from [Oryza sativa] (68414.m08744) |
| 26 | (C) Internal Glu induction | I-NC-NC | 256385_at | At1g66580 | 60S ribosomal protein L10 (RPL10C) contains Pfam profile: PF00826: Ribosomal L10 (68414.m07565) |
| 27 | (C) Internal Glu induction | I-NC-NC | 256225_at | At1g56220 | dormancy/auxin associated family protein similar to Auxin-repressed 12.5 kDa protein (Swiss-Prot: Q05349) [Fragaria ananassa]; |
| similar to auxin-repressed protein (GI: 927034) [Fragaria x ananassa]; similar to dormancy-associated protein (GI: 2605887) [Pisum | |||||
| sativum] (68414.m06461) | |||||
| 28 | (C) Internal Glu induction | I-NC-NC | 256061_at | At1g07040 | expressed protein (68414.m00750) |
| 29 | (C) Internal Glu induction | I-NC-NC | 254923_at | At4g11240 | serine/threonine protein phosphatase PP1 isozyme 6 (PP1BG) (TOPP6) identical to SP|P48486 Serine/threonine protein |
| phosphatase PP1 isozyme 6 (EC 3.1.3.16) {Arabidopsis thaliana} (68417.m01820) | |||||
| 30 | (C) Internal Glu induction | I-NC-NC | 254831_at | At4g12600 | ribosomal protein L7Ae/L30e/S12e/Gadd45 family protein Similar to NHP2/L7Ae family proteins, see SWISSPROT: P32495 and |
| PMID: 2063628. (68417.m01986) | |||||
| 31 | (C) Internal Glu induction | I-NC-NC | 254318_at | At4g22530 | embryo-abundant protein-related similar to embryo-abundant protein [Picea glauca] GI: 1350531 (68417.m03251) |
| 32 | (C) Internal Glu induction | I-NC-NC | 253203_at | At4g34710 | arginine decarboxylase 2 (SPE2) identical to SP|O23141 Arginine decarboxylase 2 (EC 4.1.1.19) (ARGDC 2) (ADC 2) (ADC-N) |
| {Arabidopsis thaliana} (68417.m04926) | |||||
| 33 | (C) Internal Glu induction | I-NC-NC | 253043_at | At4g37540 | LOB domain protein 39/lateral organ boundaries domain protein 39 (LBD39) identical to SP|Q9SZE8 LOB domain protein 39 |
| {Arabidopsis thaliana} (68417.m05312) | |||||
| 34 | (C) Internal Glu induction | I-NC-NC | 252991_at | At4g38470 | protein kinase family protein similar to protein kinase [gi: 170047] from Glycine max; contains Pfam protein kinase domain PF00069 |
| (68417.m05436) | |||||
| 35 | (C) Internal Glu induction | I-NC-NC | 252882_at | At4g39675 | expressed protein (68417.m05612) |
| 36 | (C) Internal Glu induction | I-NC-NC | 252293_at | At3g48990 | AMP-dependent synthetase and ligase family protein similar to peroxisomal-coenzyme A synthetase (FAT2) [gi: 586339] from |
| Saccharomyces cerevisiae; contains Pfam AMP-binding enzyme domain PF00501; identical to cDNA; identical to cDNA adenosine | |||||
| monophosphate binding protein 3 AMPBP3 (AMPBP3)GI: 20799714 (68416.m05351) | |||||
| 37 | (C) Internal Glu induction | I-NC-NC | 252034_at | At3g52040 | expressed protein (68416.m05708) |
| 38 | (C) Internal Glu induction | I-NC-NC | 251486_at | At3g59540 | 60S ribosomal protein L38 (RPL38B) 60S RIBOSOMAL PROTEIN L38 - Lycopersicon esculentum, EMBL X69979 (68416.m06645) |
| 39 | (C) Internal Glu induction | I-NC-NC | 250670_at | At5g06860 | polygalacturonase inhibiting protein 1 (PGIP1) identical to polygalacturonase inhibiting protein 1 (PGIP1) [Arabidopsis thaliana] |
| gi|7800199|gb|AAF69827; contains leucine rich-repeat (LRR) domains Pfam: PF00560, INTERPRO: IPR001611 (68418.m00776) | |||||
| 40 | (C) Internal Glu induction | I-NC-NC | 250385_at | At5g11520 | aspartate aminotransferase, chloroplast/transaminase A (ASP3) (YLS4) identical to SP|P46644 Aspartate aminotransferase, |
| chloroplast precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis thaliana}; identical to cDNA YLS4 mRNA for aspartate | |||||
| aminotransferase (ASP3), partial cds GI: 13122285 (68418.m01344) | |||||
| 41 | (C) Internal Glu induction | I-NC-NC | 250032_at | At5g18170 | glutamate dehydrogenase 1 (GDH1) identical to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis thaliana] SWISS- |
| PROT: Q43314 (68418.m02133) | |||||
| 42 | (C) Internal Glu induction | I-NC-NC | 249941_at | At5g22270 | expressed protein similar to unknown protein (gb AAF02129.1) (68418.m02595) |
| 43 | (C) Internal Glu induction | I-NC-NC | 249752_at | At5g24660 | expressed protein (68418.m02914) |
| 44 | (C) Internal Glu induction | I-NC-NC | 249467_at | At5g39610 | no apical meristem (NAM) family protein contains Pfam PF02365: No apical meristem (NAM) protein; similar to cup-shaped |
| cotyledon CUC2 (GI: 1944132) [Arabidopsis thaliana] (68418.m04797) | |||||
| 45 | (C) Internal Glu induction | I-NC-NC | 249125_at | At5g43450 | 2-oxoglutarate-dependent dioxygenase, putative similar to 2A6 (GI: 599622) and tomato ethylene synthesis regulatory protein E8 |
| (SP|P10967) (68418.m05312) | |||||
| 46 | (C) Internal Glu induction | I-NC-NC | 249076_at | At5g43970 | expressed protein (68418.m05380) |
| 47 | (C) Internal Glu induction | I-NC-NC | 248607_at | At5g49480 | sodium-inducible calcium-binding protein (ACP1)/sodium-responsive calcium-binding protein (ACP1) identical to NaCl-inducible |
| Ca2+-binding protein GI: 2352828 from [Arabidopsis thaliana] (68418.m06123) | |||||
| 48 | (C) Internal Glu induction | I-NC-NC | 248381_at | At5g51830 | pfkB-type carbohydrate kinase family protein contains Pfam profile: PF00294 pfkB family carbohydrate kinase (68418.m06426) |
| 49 | (C) Internal Glu induction | I-NC-NC | 247283_at | At5g64250 | 2-nitropropane dioxygenase family/NPD family contains Pfam profile PF03060: oxidoreductase, 2-nitropropane dioxygenase |
| (NPD) family (68418.m08071) | |||||
| 50 | (C) Internal Glu induction | I-NC-NC | 247009_at | At5g67600 | expressed protein (68418.m08524) |
| 51 | (C) Internal Glu induction | I-NC-NC | 246745_at | At5g27770 | 60S ribosomal protein L22 (RPL22C) ribosomal protein L22 (cytosolic), Rattus norvegicus, PIR: S52084 (68418.m03330) |
| 52 | (C) Internal Glu induction | I-NC-NC | 246463_at | At5g16970 | NADP-dependent oxidoreductase, putative (P1) identical to probable NADP-dependent oxidoreductase P1, zeta-crystallin homolog |
| [SP|Q39172][gi: 886428], Arabidopsis thaliana similar to allyl alcohol dehydrogenase [Nicotiana tabacum] GI: 6692816; contains | |||||
| Pfam profile PF00107: oxidoreductase, zinc-binding dehydrogenase family (68418.m01988) | |||||
| 53 | (C) Internal Glu induction | I-NC-NC | 245794_at | At1g32170 | xyloglucan:xyloglucosyl transferase, putative/xyloglucan endotransglycosylase, putative/endo-xyloglucan transferase, putative |
| (XTR4) identical to N-terminal partial sequence of xyloglucan endotransglycosylase-related protein XTR4 GI: 1244754 from | |||||
| [Arabidopsis thaliana]; similar to endoxyloglucan transferase [Arabidopsis thaliana] GI: 5533311 (68414.m03957) | |||||
| 54 | (C) Internal Glu induction | I-NC-NC | 245765_at | At1g33600 | leucine-rich repeat family protein contains leucine rich-repeat (LRR) domains Pfam: PF00560. INTERPRO: IPR001611; contains |
| similarity to gi|9294355|dbj|BAB02252 [Arabidopsis thaliana] (68414.m04159) | |||||
| 55 | (C) Internal Glu induction | I-NC-NC | 245512_at | At4g15770 | 60S ribosome subunit biogenesis protein, putative contains similarity to 60S ribosome subunit biogenesis protein NIP7 (Swiss- |
| Prot: Q08962) [Saccharomyces cerevisiae] (68417.m02401) | |||||
| 56 | (C) Internal Glu induction | I-NC-NC | 245038_at | At2g26560 | patatin, putative similar to patatin-like latex allergen [Hevea brasiliensis][PMID: 10589016]; contains patatin domain PF01734 |
| (68415.m03186) | |||||
| 57 | (C) Internal Glu induction | NC-D-D | 267209_at | At2g30930 | expressed protein (68415.m03771) |
| 58 | (C) Internal Glu induction | NC-D-D | 265066_at | At1g03870 | fasciclin-like arabinogalactan-protein (FLA9) identical to gi_13377784_gb_AAK20861 (68414.m00371) |
| 59 | (C) Internal Glu induction | NC-D-D | 264672_at | At1g09750 | chloroplast nucleoid DNA-binding protein-related contains Pfam profile PF00026: Eukaryotic aspartyl protease; b similar to CND41, |
| chloroplast nucleoid DNA binding protein (GI: 2541876) [Nicotiana tabacum] (68414.m01094) | |||||
| 60 | (C) Internal Glu induction | NC-D-D | 264371_at | At1g12090 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein similar to 14 kDa polypeptide [Catharanthus roseus] |
| GI: 407410; contains Pfam protease inhibitor/seed storage/LTP family domain PF00234 (68414.m01399) | |||||
| 61 | (C) Internal Glu induction | NC-D-D | 263598_at | At2g01850 | xyloglucan:xyloglucosyl transferase/xyloglucan endotransglycosylase/endo-xyloglucan transferase (EXGT-A3) identical to |
| endoxyloglucan transferase [Arabidopsis thaliana] GI: 5533313 (68415.m00118) | |||||
| 62 | (C) Internal Glu induction | NC-D-D | 261975_at | At1g64640 | plastocyanin-like domain-containing protein contains InterPro: IPR003245 plastocyanin-like domain (68414.m07328) |
| 63 | (C) Internal Glu induction | NC-D-D | 261825_at | At1g11545 | xyloglucan:xyloglucosyl transferase, putative/xyloglucan endotransglycosylase, putative/endo-xyloglucan transferase, putative |
| similar to endo-xyloglucan transferase GI: 2244732 from [Gossypium hirsutum] (68414.m01326) | |||||
| 64 | (C) Internal Glu induction | NC-D-D | 260221_at | At1g74670 | gibberellin-responsive protein, putative similar to SP|P48890 Gibberellin-regulated protein 4 precursor {Arabidopsis thaliana} |
| GASA4; contains Pfam profile PF02704: Gibberellin regulated protein (68414.m08647) | |||||
| 65 | (C) Internal Glu induction | NC-D-D | 259980_at | At1g76520 | auxin efflux carrier family protein contains auxin efflux carrier domain. Pfam: PF03547 (68414.m08904) |
| 66 | (C) Internal Glu induction | NC-D-D | 259803_at | At1g72150 | SEC14 cytosolic factor family protein/phosphoglyceride transfer family protein similar to SEC14-like protein 2 (Alpha-tocopherol |
| associated protein) (TAP) (bTAP) (Fragment) (SP: P58875) {Bos taurus}; similar to GI: 807956 from [Saccharomyces cerevisiae]; | |||||
| contains Pfam PF00650: CRAL/TRIO domain; contains Pfam PF03765: CRAL/TRIO, N-terminus (68414.m08342) | |||||
| 67 | (C) Internal Glu induction | NC-D-D | 259588_at | At1g27930 | expressed protein contains Pfam profile PF04669: Protein of unknown function (DUF579) (68414.m03422) |
| 68 | (C) Internal Glu induction | NC-D-D | 258410_at | At3g16780 | 60S ribosomal protein L19 (RPL19B) similar to ribosomal protein L19 GB: CAA45090 from [Homo sapiens] (68416.m02142) |
| 69 | (C) Internal Glu induction | NC-D-D | 258369_at | At3g14310 | pectinesterase family protein contains Pfam profiles: PF01095 pectinesterase, PF04043 plant invertase/pectin methylesterase |
| inhibitor; similar to pectin methylesterase GB: Q42534 from [Arabidopsis thaliana] (68416.m01810) | |||||
| 70 | (C) Internal Glu induction | NC-D-D | 257798_at | At3g15950 | DNA topoisomerase-related similar to DNA topoisomerase IV subunit A (GI: 26454107) [Mycoplasma penetrans] (68416.m02017) |
| 71 | (C) Internal Glu induction | NC-D-D | 257203_at | At3g23730 | xyloglucan:xyloglucosyl transferase, putative/xyloglucan endotransglycosylase, putative/endo-xyloglucan transferase, putative |
| similar to xyloglucan endotransglycosylase-related protein GI: 1244780 from [Arabidopsis thaliana] (68416.m02984) | |||||
| 72 | (C) Internal Glu induction | NC-D-D | 257037_at | At3g19130 | RNA-binding protein, putative similar to RNA Binding Protein 47 [Nicotiana plumbaginifolia] GI: 9663769, DNA binding |
| protein ACBF GB: AAC49850 from [Nicotiana tabacum]; contains InterPro entry IPR000504: RNA-binding region RNP-1 | |||||
| (RNA recognition motif) (RRM) (68416.m02429) | |||||
| 73 | (C) Internal Glu induction | NC-D-D | 256543_at | At1g42480 | expressed protein (68414.m04898) |
| 74 | (C) Internal Glu induction | NC-D-D | 255856_at | At1g66940 | protein kinase-related (68414.m07608) |
| 75 | (C) Internal Glu induction | NC-D-D | 254789_at | At4g12880 | plastocyanin-like domain-containing protein (68417.m02016) |
| 76 | (C) Internal Glu induction | NC-D-D | 254726_at | At4g13660 | pinoresinol-lariciresinol reductase, putative similar to pinoresinol-lariciresinol reductase TH1 [Tsuga heterophylla][GI: 7578915]; |
| contains isoflavone reductase domain PF02718 (68417.m02124) | |||||
| 77 | (C) Internal Glu induction | NC-D-D | 252407_at | At3g47520 | malate dehydrogenase [NAD], chloroplast (MDH) identical to chloroplast NAD-malate dehydrogenase [Arabidopsis thaliana] |
| GI: 3256066; contains InterPro entry IPR001236: Lactate/malate dehydrogenase; contains Pfam profiles PF00056: lactate/malate | |||||
| dehydrogenase, NAD binding domain and PF02866: lactate/malate dehydrogenase, alpha/beta C-terminal domain | |||||
| (68416.m05168) | |||||
| 78 | (C) Internal Glu induction | NC-D-D | 252220_at | At3g49940 | LOB domain protein 38/lateral organ boundaries domain protein 38 (LBD38) identical to SP|Q9SN23 LOB domain protein 38 |
| {Arabidopsis thaliana} (68416.m05461) | |||||
| 79 | (C) Internal Glu induction | NC-D-D | 252199_at | At3g50270 | transferase family protein similar to anthranilate N-hydroxycinnamoyl/benzoyltransferase, Dianthus caryophyllus [gi: 2239091]; |
| contains Pfam transferase family domain PF002458 (68416.m05497) | |||||
| 80 | (C) Internal Glu induction | NC-D-D | 251830_at | At3g55010 | phosphoribosylformylglycinamidine cyclo-ligase. chloroplast/phosphoribosyl-aminoimidazole synthetase/AIR synthase (PUR5) |
| identical to phosphoribosylformylglycinamidine cyclo-ligase, chloroplast precursor SP: Q05728 from [Arabidopsis thaliana]; contains | |||||
| Pfam profiles: PF02769 AIR synthase related protein, C-terminal domain, PF00586 AIR synthase related protein, N-terminal | |||||
| domain (68416.m06108) | |||||
| 81 | (C) Internal Glu induction | NC-D-D | 251015_at | At5g02480 | expressed protein p (68418.m00181) |
| 82 | (C) Internal Glu induction | NC-D-D | 250777_at | At5g05440 | expressed protein low similarity to cytokinin-specific binding protein [Vigna radiata] GI: 4190976 (68418.m00586) |
| 83 | (C) Internal Glu induction | NC-D-D | 250366_at | At5g11420 | expressed protein contains Pfam profile PF04862: Protein of unknown function, DUF642 (68418.m01333) |
| 84 | (C) Internal Glu induction | NC-D-D | 248564_at | At5g49700 | DNA-binding protein-related contains Pfam domain PF03479: Domain of unknown function (DUF296), found in AT-hook motifs |
| Pfam: PF02178 (68418.m06153) | |||||
| 85 | (C) Internal Glu induction | NC-D-D | 247555_at | At5g61020 | YT521-B-like family protein contains Pfam profile PF04146: YT521-B-like family (68418.m07655) |
| 86 | (C) Internal Glu induction | NC-D-D | 247266_at | At5g64570 | glycosyl hydrolase family 3 protein (68418.m08115) |
| 87 | (C) Internal Glu induction | NC-D-D | 246919_at | At5g25460 | expressed protein contains Pfam profile PF04862: Protein of unknown function, DUF642 (68418.m03026) |
| 88 | (C) Internal Glu induction | NC-D-D | 246550_at | At5g14920 | gibberellin-regulated family protein similar to SP|P46689 Gibberellin-regulated protein 1 precursor (Arabidopsis thaliana); contains |
| Pfam profile PF02704: Gibberellin regulated protein (68418.m01750) | |||||
| 89 | (C) Internal Glu induction | NC-D-D | 245164_at | At2g33210 | chaperonin, putative similar to SWISS-PROT: Q05046-chaperonin CPN60-2, mitochondrial precursor (HSP60-2) [Cucurbita |
| maxima]; contains Pfam: PF00118 domain, TCP-1/cpn60 chaperonin family (68415.m04069) | |||||
| 90 | (C) Internal Glu Repression | D-I-I | 264213_at | At1g65400 | |
| 91 | (C) Internal Glu Repression | D-I-I | 245329_at | At4g14365 | zinc finger (C3HC4-type RING finger) family protein/ankyrin repeat family protein contains Pfam profile: PF00097 zinc finger, |
| C3HC4 type (RING finger) and Pfam profile: PF00023 ankyrin repeat (68417.m02213) | |||||
| 92 | (C) Internal Glu Repression | D-NC-NC | 267470_at | At2g30490 | trans-cinnamate 4-monooxygenase/cinnamic acid 4-hydroxylase (C4H) (CA4H)/cytochrome P450 73 (CYP73) (CYP73A5) |
| identical to SP|P92994| Trans-cinnamate 4-monooxygenase (EC 1.14.13.11) (Cinnamic acid 4-hydroxylase) (CA4H) (C4H) | |||||
| (P450C4H) (Cytochrome P450 73). {Arabidopsis thaliana}; molecular marker C4H (GB: U71080) (68415.m03714) | |||||
| 93 | (C) Internal Glu Repression | D-NC-NC | 267367_at | At2g44210 | expressed protein Pfam profile PF03080: Arabidopsis proteins of unknown function (68415.m05502) |
| 94 | (C) Internal Glu Repression | D-NC-NC | 267187_s_at | At2g44160 | methylenetetrahydrofolate reductase 2 (MTHFR2) identical to SP|O80585 Methylenetetrahydrofolate reductase (EC 1.5.1.20) |
| {Arabidopsis thaliana} (68415.m05493) | |||||
| 95 | (C) Internal Glu Repression | D-NC-NC | 267154_at | At2g30870 | glutathione S-transferase, putative supported by cDNA GI: 443698 GB: D17673 (68415.m03762) |
| 96 | (C) Internal Glu Repression | D-NC-NC | 267136_at | At2g23460 | extra-large guanine nucleotide binding protein/G-protein (XLG) identical to extra-large G-protein (XLG) [Arabidopsis thaliana] |
| GI: 3201680 (68415.m02801) | |||||
| 97 | (C) Internal Glu Repression | D-NC-NC | 266993_at | At2g39210 | nodulin family protein similar to nodulin-like protein [Arabidopsis thaliana] GI: 3329368, nodule-specific protein Nlj70 [Lotus |
| japonicus] GI: 3329366 (68415.m04816) | |||||
| 98 | (C) Internal Glu Repression | D-NC-NC | 266967_at | At2g39530 | integral membrane protein, putative contains 3 transmembrane domains; contains Pfam PF04535: Domain of unknown function |
| (DUF588): similar to putative ethylene responsive element binding protein (GI: 22135858) [Arabidopsis thaliana] (68415.m04850) | |||||
| 99 | (C) Internal Glu Repression | D-NC-NC | 266857_at | At2g26900 | bile acid:sodium symporter family protein low similarity to SP|Q12908 lleal sodium/bile acid cotransporter {Homo sapiens}; contains |
| Pfam profile PF01758: Sodium Bile acid symporter family (68415.m03227) | |||||
| 100 | (C) Internal Glu Repression | D-NC-NC | 266808_at | At2g29995 | expressed protein (68415.m03649) |
| 101 | (C) Internal Glu Repression | D-NC-NC | 266719_at | At2g46830 | myb-related transcription factor (CCA1) identical to myb-related transcription factor (CCA1) GI: 4090569 from [Arabidopsis thaliana] |
| (68415.m05843) | |||||
| 102 | (C) Internal Glu Repression | D-NC-NC | 266591_at | At2g46225 | expressed protein (68415.m05748) |
| 103 | (C) Internal Glu Repression | D-NC-NC | 266516_at | At2g47880 | glutaredoxin family protein contains INTERPRO Domain IPR002109, Glutaredoxin (thioltransferese) (68415.m05979) |
| 104 | (C) Internal Glu Repression | D-NC-NC | 266414_at | At2g38700 | mevalonate diphosphate decarboxylase (MVD1) identical to mevalonate diphosphate decarboxylase [Arabidopsis thaliana] |
| gi|2288887|emb|CAA74700 (68415.m04753) | |||||
| 105 | (C) Internal Glu Repression | D-NC-NC | 266295_at | At2g29550 | tubulin beta-7 chain (TUB7) identical to GB: M84704 SP|P29515 Tubulin beta-7 chain {Arabidopsis thaliana} (68415.m03589) |
| 106 | (C) Internal Glu Repression | D-NC-NC | 266205_s_at | At2g27860 | expressed protein (68415.m03377) |
| 107 | (C) Internal Glu Repression | D-NC-NC | 266089_at | At2g38010 | ceramidase family protein contains Pfam domain, PF04734: Neutral/alkaline nonlysosomal ceramidase (68415.m04665) |
| 108 | (C) Internal Glu Repression | D-NC-NC | 265286_at | At2g20360 | expressed protein (68415.m02377) |
| 109 | (C) Internal Glu Repression | D-NC-NC | 265208_at | At2g36690 | oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to IDS3 [Hordeum vulgare][GI: 4514655], leucoanthocyanidin |
| dioxygenase [SP|P51091][Malus domestica]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain (68415.m04501) | |||||
| 110 | (C) Internal Glu Repression | D-NC-NC | 264914_at | At1g60780 | clathrin adaptor complexes medium subunit family protein contains Pfam profile: PF00928 adaptor complexes medium subunit |
| family (68414.m06842) | |||||
| 111 | (C) Internal Glu Repression | D-NC-NC | 264833_at | At1g03590 | protein phosphatase 2C family protein/PP2C family protein similar to protein phosphatase-2C; PP2C (GI: 3643088) |
| [Mesembryanthemum crystallinum]; contains Pfam PF00481: Protein phosphatase 2C domain; (68414.m00339) | |||||
| 112 | (C) Internal Glu Repression | D-NC-NC | 264434_at | At1g10340 | ankyrin repeat family protein contains ankyrin repeat domains, Pfam: PF00023 (68414.m01164) |
| 113 | (C) Internal Glu Repression | D-NC-NC | 264386_at | At1g12000 | pyrophosphate-fructose-6-phosphate 1-phosphotransferase beta subunit, putative/pyrophosphate-dependent 6-phosphofructose- |
| 1-kinase, putative strong similarity to SP|Q41141 Pyrophosphate-fructose 6-phosphate 1-phosphotransferase beta subunit (EC | |||||
| 2.7.1.90) (PFP) ((PPI-PFK) {Ricinus communis}; contains Pfam profile PF00365: Phosphofructokinase (68414.m01386) | |||||
| 114 | (C) Internal Glu Repression | D-NC-NC | 264372_at | At1g11840 | lactoylglutathione lyase, putative/glyoxalase I, putative highly similar to putative lactoylglutathione lyase SP: Q39366 from |
| [Brassica oleracea] (68414.m01360) | |||||
| 115 | (C) Internal Glu Repression | D-NC-NC | 264316_at | At1g70330 | equilibrative nucleoside transporter family protein contains similarity to SWISS-PROT: Q14542 equilibrative nucleoside transporter 2 |
| (Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter, Equilibrative NBMPR-insensitive nucleoside | |||||
| transporter, Nucleoside transporter, ei-type, 38 kDa nucleolar protein HNP36, Hydrophobic nucleolar protein, 36 kDa, Delayed-early | |||||
| response protein 12) [Homo sapiens] (68414.m08091) | |||||
| 116 | (C) Internal Glu Repression | D-NC-NC | 264057_at | At2g28550 | AP2 domain-containing transcription factor RAP2.7 (RAP2.7) nearly identical to AP2 domain transcription factor RAP2.7 |
| (GI: 2281639) [Arabidopsis thaliana] (68415.m03468) | |||||
| 117 | (C) Internal Glu Repression | D-NC-NC | 264005_at | At2g22470 | arabinogalactan-protein (AGP2) identical to gi|3883122|gb|AAC77824; supported by cDNA gi|3883121|gb|AF082299 |
| (68415.m02664) | |||||
| 118 | (C) Internal Glu Repression | D-NC-NC | 264006_at | At2g22430 | homeobox-leucine zipper protein 6 (HB-6)/HD-ZIP transcription factor 6 identical to homeobox-leucine zipper protein ATHB-6 (HD- |
| ZIP protein ATHB-6) (SP: P46668) [Arabidopsis thaliana] (68415.m02660) | |||||
| 119 | (C) Internal Glu Repression | D-NC-NC | 263935_at | At2g35930 | U-box domain-containing protein similar to immediate-early fungal elicitor protein CMPG1 [Petroselinum crispum] GI: 14582200; |
| contains Pfam profile PF04564: U-box domain (68415.m04410) | |||||
| 120 | (C) Internal Glu Repression | D-NC-NC | 263900_at | At2g36290 | hydrolase, alpha/beta fold family protein low similarity to 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase [Rhodococcus sp. |
| RHA1] GI: 8978311; contains Pfam profile PF00561: hydrolase, alpha/beta fold family (68415.m04453) | |||||
| 121 | (C) Internal Glu Repression | D-NC-NC | 263804_at | At2g40270 | protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain (68415.m04954) |
| 122 | (C) Internal Glu Repression | D-NC-NC | 263715_at | At2g20570 | golden2-like transcription factor (GLK1) identical to golden2-like transcription factor GI: 13311003 from [Arabidopsis thaliana]; |
| contains Pfam profile: PF00249 Myb-like DNA-binding domain (68415.m02402) | |||||
| 123 | (C) Internal Glu Repression | D-NC-NC | 263626_at | At2g04880 | WRKY family transcription factor (ZAP1) identical to ZAP1 GI: 1064883 from [Arabidopsis thaliana]; contains Pfam profile: PF03106 |
| WRKY DNA-binding domain (68415.m06038) | |||||
| 124 | (C) Internal Glu Repression | D-NC-NC | 263553_at | At2g16430 | purple acid phosphatase (PAP10) identical to purple acid phosphatase (PAP10) GI: 20257482 from [Arabidopsis thaliana] |
| (68415.m01881) | |||||
| 125 | (C) Internal Glu Repression | D-NC-NC | 263536_at | At2g25000 | WRKY family transcription factor contains Pfam profile: PF03106 WRKY DNA-binding domain (68415.m02989) |
| 126 | (C) Internal Glu Repression | D-NC-NC | 263433_at | At2g22240 | inositol-3-phosphate synthase isozyme 2/myo-inositol-1-phosphate synthase 2/MI-1-P synthase 2/IPS 2 identical to SP|Q38862 |
| Myo-inositol-1-phosphate synthase isozyme 2 (EC 5.5.1.4) (MI-1-P synthase 2) (IPS 2) {Arabidopsis thaliana} (68415.m02639) | |||||
| 127 | (C) Internal Glu Repression | D-NC-NC | 262980_at | At1g75680 | glycosyl hydrolase family 9 protein similar to endo-beta-1,4-glucanase GB: AAC12685 GI: 3025470 from [Pinus radiata] |
| (68414.m08792) | |||||
| 128 | (C) Internal Glu Repression | D-NC-NC | 262919_at | At1g79380 | copine-related low similarity to SP|Q99829 Copine I {Homo sapiens} (68414.m09251) |
| 129 | (C) Internal Glu Repression | D-NC-NC | 262637_at | At1g06640 | 2-oxoglutarate-dependent dioxygenase, putative similar to 2AB (GI: 599622) and tomato ethylene synthesis regulatory protein E8 |
| (SP|P10967); contains Pfam profile: PF00671 Iron/Ascorbate oxidoreductase family (68414.m00701) | |||||
| 130 | (C) Internal Glu Repression | D-NC-NC | 262588_at | At1g15130 | hydroxyproline-rich glycoprotein family protein (68414.m01807) |
| 131 | (C) Internal Glu Repression | D-NC-NC | 262376_at | At1g72970 | glucose-methanol-choline (GMC) oxidoreductase family protein similar to mandelonitrile lyase from Prunus serotina [SP|P52706, |
| SP|P52707]; contains Pfam profile PF00732 GMC oxidoreductase (68414.m08439) | |||||
| 132 | (C) Internal Glu Repression | D-NC-NC | 262313_at | At1g70900 | expressed protein (68414.m08181) |
| 133 | (C) Internal Glu Repression | D-NC-NC | 262197_at | At1g53910 | AP2 domain-containing protein RAP2.12 (RAP2.12) identical to AP2 domain containing protein GI: 2281649 from [Arabidopsis |
| thaliana] (68414.m06137) | |||||
| 134 | (C) Internal Glu Repression | D-NC-NC | 262177_at | At1g74710 | isochorismate synthase 1 (ICS1)/isochorismate mutase identical to GI: 17223087 and GB: AF078080; contains Pfam profile |
| PF00425: chorismate binding enzyme; contains TIGRfam profile TIGR00543: isochorismate synthases; identical to cDNA | |||||
| isochorismate synthase 1 precursor (ICS1) nuclear gene for plastid product GI: 17223086 (68414.m08654) | |||||
| 135 | (C) Internal Glu Repression | D-NC-NC | 262051_at | At1g79990 | coatomer protein complex, subunit beta 2 (beta prime), putative contains 7 WD-40 repeats (PF00400) (1 weak); similar to |
| Coatomer beta′ subunit (Beta′-coat protein) (Beta′-COP) (p102) (SP: P35606) [Homo sapiens]; similar to Coatomer beta′ subunit | |||||
| (Beta′-coat protein) (Beta′-COP) (p102) (SP: O55029) [Mus musculus] (68414.m09356) | |||||
| 136 | (C) Internal Glu Repression | D-NC-NC | 262052_at | At1g80020 | |
| 137 | (C) Internal Glu Repression | D-NC-NC | 261970_at | At1g65960 | glutamate decarboxylase 2 (GAD 2) similar to glutamate decarboxylase (gad) GI: 294111 from [Petunia hybrida] (68414.m07484) |
| 138 | (C) Internal Glu Repression | D-NC-NC | 261935_at | At1g22610 | C2 domain-containing protein contains INTERPRO: IPR000008 C2 domain (68414.m02823) |
| 139 | (C) Internal Glu Repression | D-NC-NC | 261792_at | At1g15950 | cinnamoyl-CoA reductase, putative nearly identical to CCR1 (GI: 12034897), similar to cinnamoyl CoA reductase GI: 2058310 from |
| [Eucalyptus gunnii] (68414.m01914) | |||||
| 140 | (C) Internal Glu Repression | D-NC-NC | 261469_at | At1g28340 | leucine-rich repeat family protein contains leucine rich-repeat (LRR) domains Pfam: PF00560, INTERPRO: IPRO01611; contains |
| some similarity to receptor-like protein kinases (68414.m03481) | |||||
| 141 | (C) Internal Glu Repression | D-NC-NC | 261412_at | At1g07890 | L-ascorbate peroxidase 1, cytosolic (APX1) identical to SP|Q05431 L-ascorbate peroxidase, cytosolic (EC 1.11.1.11) (AP) |
| {Arabidopsis thaliana}, L-ascorbate peroxidase [Arabidopsis thaliana] gi|16173|emb|CAA42168; strong similarity to cytosolic | |||||
| ascorbate peroxidase [Spinacia oleracea] gi|1384110|dbj|BAA12890 (68414.m00856) | |||||
| 142 | (C) Internal Glu Repression | D-NC-NC | 261286_at | At1g35780 | expressed protein (68414.m04448) |
| 143 | (C) Internal Glu Repression | D-NC-NC | 260985_at | At1g53500 | NAD-dependent epimerase/dehydratase family protein low similarity to dTDP-D-glucose-4,6-dehydratase from Aneurinibacillus |
| thermoaerophilus GI: 16357481, Sphingomonas sp. GI: 1314581; contains Pfam profile PF01370: NAD dependent | |||||
| epimerase/dehydratase family; putative NDP-rhamnose synthase (rhm2 gene) GI: 31559258 (68414.m06066) | |||||
| 144 | (C) Internal Glu Repression | D-NC-NC | 260880_at | At1g21380 | VHS domain-containing protein/GAT domain-containing protein weak similarity to Hrs [Rattus norvegicus] GI: 8547026; contains |
| Pfam profiles PF00790: VHS domain, PF03127: GAT domain (68414.m02875) | |||||
| 145 | (C) Internal Glu Repression | D-NC-NC | 260839_at | At1g43690 | ubiquitin interaction motif-containing protein contains Pfam profile PF02809: Ubiquitin interaction motif (68414.m05019) |
| 146 | (C) Internal Glu Repression | D-NC-NC | 260800_at | At1g78240 | dehydration-responsive protein-related similar to early-responsive to dehydration stress ERD3 protein [Arabidopsis thaliana] |
| GI: 15320410; contains Pfam profile PF03141: Putative methyltransferase (68414.m09118) | |||||
| 147 | (C) Internal Glu Repression | D-NC-NC | 260693_at | At1g32450 | proton-dependent oligopeptide transport (POT) family protein contains Pfam profile: PF00854 POT family (68414.m04005) |
| 148 | (C) Internal Glu Repression | D-NC-NC | 260618_at | At1g53230 | TCP family transcription factor 3 (TCP3) identical to transcription factor 3 (TCP3) [Arabidopsis thaliana] (GI: 3243274); similar to |
| flower development protein cycloidea (cyc3) GI: 6358611 from [Misopates orontium] (68414.m06032) | |||||
| 149 | (C) Internal Glu Repression | D-NC-NC | 260590_at | At1g53310 | phosphoenolpyruvate carboxylase, putative/PEP carboxylase, putative (PPC1) strong similarity to SP|P29196 |
| Phosphoenolpyruvate carboxylase (EC 4.1.1.31) (PEPCASE) {Solanum tuberosum}; contains Pfam profile PF00311: | |||||
| phosphoenolpyruvate carboxylase (68414.m06042) | |||||
| 150 | (C) Internal Glu Repression | D-NC-NC | 260601_at | At1g55910 | metal transporter, putative (ZIP11) similar to putative zinc transporter ZIP2 [Arabidopsis thaliana] gi|3252868|gb|AAC24198; |
| member of the Zinc (Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID: 11500563 (68414.m06412) | |||||
| 151 | (C) Internal Glu Repression | D-NC-NC | 260401_at | At1g69840 | band 7 family protein strong similarity to hypersensitive-induced response protein [Zea mays] GI: 7716466; contains Pfam profile |
| PF01145: SPFH domain/Band 7 family (68414.m08035) | |||||
| 152 | (C) Internal Glu Repression | D-NC-NC | 260234_at | At1g74460 | GDSL-motif lipase/hydrolase family protein similar to family II lipases EXL3 GI: 15054386, EXL1 GI: 15054382, EXL2 GI: 15054384 |
| from [Arabidopsis thaliana]; contains Pfam profile: PF00657 lipase/acylhydrolase with GDSL-like motif (68414.m08826) | |||||
| 153 | (C) Internal Glu Repression | D-NC-NC | 260048_at | At1g73800 | calmodulin-binding protein similar to calmodulin-binding protein TCB60 GI: 1698548 from [Nicotiana tabacum] (68414.m08544) |
| 154 | (C) Internal Glu Repression | D-NC-NC | 259882_at | At1g76670 | transporter-related low similarity to glucose-6-phosphate/phosphate-translocator precursor [Solanum tuberosum] GI: 2997593. GDP- |
| Mannose transporter [Arabidopsis thaliana] GI: 15487237; contains Pfam profile PF00892: Integral membrane protein | |||||
| (68414.m08921) | |||||
| 155 | (C) Internal Glu Repression | D-NC-NC | 259561_at | At1g21250 | wall-associated kinase 1 (WAK1) identical to wall-associated kinase 1 [Arabidopsis thaliana] GI: 3549626; expressed in leaves and |
| stems & induced by pathogen infection (PMID: 10380805) (68414.m02656) | |||||
| 156 | (C) Internal Glu Repression | D-NC-NC | 259507_at | At1g43910 | AAA-type ATPase family protein contains Pfam profile: ATPase family PF00004 (68414.m05086) |
| 157 | (C) Internal Glu Repression | D-NC-NC | 259383_at | At3g16470 | jacalin lectin family protein contains Pfam profile: PF01419 jacalin-like lectin domain; similar to myrosinase-binding protein homolog |
| [Arabidopsis thaliana] GI: 2997767 (68416.m02101) | |||||
| 158 | (C) Internal Glu Repression | D-NC-NC | 259175_at | At3g01560 | proline-rich family protein contains proline rich extensin domains, INTERPRO: IPR002965 (68416.m00086) |
| 159 | (C) Internal Glu Repression | D-NC-NC | 259070_at | At3g11670 | digalactosyldiacylglycerol synthase 1 (DGD1)/MGDG: MGDG galactosyltransferase/galactolipid galactosyltransferase identical to |
| digalactosyldiacylglycerol synthase (DGD1) GI: 5354158 [Arabidopsis thaliana] (68416.m01430) | |||||
| 160 | (C) Internal Glu Repression | D-NC-NC | 258925_at | At3g10420 | sporulation protein-related similar to hypothetical proteins: GB: P51281 [Chloroplast Porphyra purpurea], GB: BAA16982 |
| [Synechocystis sp], GB: P49540 [Odontella sinensis], GB: AAB82669 [Chloroplast Cyanidium caldarium]; similar to stage III | |||||
| sporulation protein AA (GI: 18145497) [Clostridium perfringens str. 13]; similar to stage III sporulation protein AA (mutants block | |||||
| sporulation after engulfment) (GI: 22777578) [Oceanobacillus iheyensis] (68416.m01249) | |||||
| 161 | (C) Internal Glu Repression | D-NC-NC | 258649_at | At3g09840 | cell division cycle protein 48 (CDC48A) (CDC48) identical to SP|P54809 Cell division cycle protein 48 homolog {Arabidopsis |
| thaliana} (68416.m01174) | |||||
| 162 | (C) Internal Glu Repression | D-NC-NC | 258621_at | At3g02830 | zinc finger (CCCH-type) family protein contains Pfam domain, PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and similar) |
| (68416.m00275) | |||||
| 163 | (C) Internal Glu Repression | D-NC-NC | 258286_at | At3g16060 | kinesin motor family protein similar to kinesin heavy chain member 2 GB: NP_032468 from [Mus musculus]; contains Pfam profile |
| PF00225: Kinesin motor domain (68416.m02030) | |||||
| 164 | (C) Internal Glu Repression | D-NC-NC | 258160_at | At3g17820 | glutamine synthetase (GS1) identical to glutamine synthetase, cytosolic isozyme (glutamate-ammonia ligase, GS1) [Arabidopsis |
| thaliana] SWISS-PROT: Q9LVI8 (68416.m02272) | |||||
| 165 | (C) Internal Glu Repression | D-NC-NC | 258037_at | At3g21230 | 4-coumarate-CoA ligase, putative/4-coumaroyl-CoA synthase, putative (4CL) similar to 4CL2 [gi: 122291365] and 4CL1 |
| [gi: 12229649] from [Arabidopsis thaliana], 4CL1 [gi: 12229631] from Nicotiana tabacum (68416.m02683) | |||||
| 166 | (C) Internal Glu Repression | D-NC-NC | 258047_at | At3g21240 | 4-coumarate-CoA ligase 2/4-coumaroyl-CoA synthase 2 (4CL2) identical to SP|Q9S725 4-coumarate-CoA ligase 2 (EC 6.2.1.12) |
| (4CL 2) (4-coumaroyl-CoA synthase 2) {Arabidopsis thaliana} (68416.m02684) | |||||
| 167 | (C) Internal Glu Repression | D-NC-NC | 258007_at | At3g19420 | expressed protein (68416.m02463) |
| 168 | (C) Internal Glu Repression | D-NC-NC | 257952_at | At3g21770 | peroxidase 30 (PER30) (P30) (PRXR9) identical to SP|Q9LSY7 Peroxidase 30 precursor (EC 1.11.1.7) (Atperox P30) (PRXR9) |
| (ATP7a) {Arabidopsis thaliana} (68416.m02746) | |||||
| 169 | (C) Internal Glu Repression | D-NC-NC | 257849_at | At3g13060 | expressed protein contains Pfam profile PF04146: YT521-B-like family (68416.m01627) |
| 170 | (C) Internal Glu Repression | D-NC-NC | 257104_at | At3g25040 | ER lumen protein retaining receptor, putative/HDEL receptor, putative similar to SP|P35402 ER lumen protein retaining receptor |
| (HDEL receptor) {Arabidopsis thaliana}; contains Pfam profile PF00810: ER lumen protein retaining receptor (68416.m03129) | |||||
| 171 | (C) Internal Glu Repression | D-NC-NC | 257076_at | At3g19680 | expressed protein (68416.m02493) |
| 172 | (C) Internal Glu Repression | D-NC-NC | 256865_at | At3g23820 | NAD-dependent epimerase/dehydratase family protein similar to nucleotide sugar epimerase from Vibrio vulnificus GI: 3093975 |
| [PID: g3093975], WbnF [Escherichia coli] GI: 5739472, CAPI protein {Staphylococcus aureus} SP|P39858; contains Pfam profile: | |||||
| PF01370 NAD dependent epimerase/dehydratase family (68416.m02994) | |||||
| 173 | (C) Internal Glu Repression | D-NC-NC | 256328_at | At3g02360 | 6-phosphogluconate dehydrogenase family protein contains Pfam profiles: PF00393 6-phosphogluconate dehydrogenase C- |
| terminal domain, PF03446 NAD binding domain of 6-phosphogluconate; similar to 6-phosphogluconate dehydrogenase | |||||
| GB: BAA22812 GI: 2529229 [Glycine max] (68416.m00219) | |||||
| 174 | (C) Internal Glu Repression | D-NC-NC | 256322_at | At1g54890 | expressed protein (68414.m06251) |
| 175 | (C) Internal Glu Repression | D-NC-NC | 256186_at | At1g51680 | 4-coumarate-CoA ligase 1/4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524 4-coumarate-CoA ligase 1 (EC 6.2.1.12) |
| (4CL 1) (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana} (68414.m05822) | |||||
| 176 | (C) Internal Glu Repression | D-NC-NC | 255953_at | At1g22070 | bZIP family transcription factor (TGA3) identical to transcription factor GI: 304113 from [Arabidopsis thaliana] (68414.m02780) |
| 177 | (C) Internal Glu Repression | D-NC-NC | 255889_at | At1g17840 | ABC transporter family protein similar to ABC transporter GI: 10280532 from [Homo sapiens] (68414.m02208) |
| 178 | (C) Internal Glu Repression | D-NC-NC | 255690_at | At4g00360 | cytochrome P450, putative (68417.m00050) |
| 179 | (C) Internal Glu Repression | D-NC-NC | 255691_at | At4g00370 | sugar transporter family protein contains Pfam profile PF00083: major facilitator superfamily protein (68417.m00051) |
| 180 | (C) Internal Glu Repression | D-NC-NC | 255671_at | At4g00355 | expressed protein (68417.m00047) |
| 181 | (C) Internal Glu Repression | D-NC-NC | 255364_s_at | At4g04020 | plastid-lipid associated protein PAP, putative/fibrillin, putative strong similarity to plastid-lipid associated proteins PAP1 |
| GI: 14248554, PAP2 GI: 14248556 from [Brassica rapa], fibrillin [Brassica napus] GI: 4139097; contains Pfam profile PF04755: | |||||
| PAP_fibrillin (68417.m00572) | |||||
| 182 | (C) Internal Glu Repression | D-NC-NC | 255332_at | At4g04340 | early-responsive to dehydration protein-related/ERD protein-related similar to ERD4 protein (early-responsive to dehydration |
| stress) [Arabidopsis thaliana] GI: 15375406; contains Pfam profile PF02714: Domain of unknown function DUF221 (68417.m00619) | |||||
| 183 | (C) Internal Glu Repression | D-NC-NC | 254874_at | At4g11570 | haloacid dehalogenase-like hydrolase family protein similar to genetic modifier [Zea mays] GI: 10444400; contains InterPro |
| accession IPR005834: Haloactd dehalogenase-like hydrolase (68417.m01854) | |||||
| 184 | (C) Internal Glu Repression | D-NC-NC | 254833_s_at | At4g12280 | copper amine oxidase family protein contains Pfam domain, PF01179: Copper amine oxidase, enzyme domain (68417.m01946) |
| 185 | (C) Internal Glu Repression | D-NC-NC | 254577_at | At4g19450 | nodulin-related weak similarity to nodule-specific protein Nlj70 [Lotus japonicus] GI: 3329366 (68417.m02861) |
| 186 | (C) Internal Glu Repression | D-NC-NC | 254543_at | At4g19810 | glycosyl hydrolase family 18 protein similar to chitinase/lysozyme GI: 487689 from [Nicotiana tabacum] (68417.m02905) |
| 187 | (C) Internal Glu Repression | D-NC-NC | 254387_at | At4g21850 | methionine sulfoxide reductase domain-containing protein/SelR domain-containing protein low similarity to pilin-like transcription |
| factor [Homo sapiens] GI: 5059062, SP|P14930 Peptide methionine sulfoxide reductase msrA/msrB (EC 1.8.4.6) {Neisseria | |||||
| gonorrhoeae}; contains Pfam profile PF01641: SelR domain (68417.m03159) | |||||
| 188 | (C) Internal Glu Repression | D-NC-NC | 254242_at | At4g23200 | protein kinase family protein contains Pfam PF00069: Protein kinase domain (68417.m03346) |
| 189 | (C) Internal Glu Repression | D-NC-NC | 254243_at | At4g23210 | protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain (68417.m03347) |
| 190 | (C) Internal Glu Repression | D-NC-NC | 254227_at | At4g23630 | reticulon family protein (RTNLB1) weak similarity to Nogo-C protein [Rattus norvegicus] GI: 6822251; contains Pfam profile |
| PF02453: Reticulon (68417.m03403) | |||||
| 191 | (C) Internal Glu Repression | D-NC-NC | 254159_at | At4g24240 | WRKY family transcription factor contains Pfam profile: PF03106 WRKY DNA-binding domain (68417.m03479) |
| 192 | (C) Internal Glu Repression | D-NC-NC | 254141_at | At4g24620 | glucose-6-phosphate isomerase, putative similar to glucose-6-phosphate isomerase [Spinacia oleracea] GI: 3413511; contains |
| Pfam profile PF00342: glucose-6-phosphate isomerase (68417.m03526) | |||||
| 193 | (C) Internal Glu Repression | D-NC-NC | 254053_s_at | At4g25300 | oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to flavonol synthase [Petunia x hybrida][GI: 311658], anthocyanidin |
| synthase [Torenia fournieri][GI: 12583673]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain (68417.m03638) | |||||
| 194 | (C) Internal Glu Repression | D-NC-NC | 254021_at | At4g25650 | Rieske [2Fe—2S] domain-containing protein similar to cell death suppressor protein lls1 from Zea mays [gi: 1935909], Rieske iron- |
| sulfur protein Tic55 from Pisum sativum [gi: 2764524]; contains Pfam PF00355 Rieske [2Fe—2S] domain (68417.m03693) | |||||
| 195 | (C) Internal Glu Repression | D-NC-NC | 253856_at | At4g28100 | expressed protein (68417.m04031) |
| 196 | (C) Internal Glu Repression | D-NC-NC | 253815_at | At4g28250 | beta-expansin, putative (EXPB3) similar to soybean pollen allergen (cim1) protein-soybean, PIR2: S48032; beta-expansin gene |
| family, PMID: 11641069 (68417.m04047) | |||||
| 197 | (C) Internal Glu Repression | D-NC-NC | 253702_at | At4g29900 | calcium-transporting ATPase, plasma membrane-type, putative/Ca2+-ATPase, putative (ACA10) identical to SP|Q9SZR1 |
| Potential calcium-transporting ATPase 10, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 10) {Arabidopsis thaliana}; | |||||
| similar to SP|Q9LF79 Calcium-transporting ATPase 8, plasma membrane-type (EC 3.6.3.8) (Ca(2+)-ATPase isoform 8) | |||||
| {Arabidopsis thaliana} (68417.m04254) | |||||
| 198 | (C) Internal Glu Repression | D-NC-NC | 253628_at | At4g30280 | xyloglucan:xyloglucosyl transferase, putative/xyloglucan endotransglycosylase, putative/endo-xyloglucan transferase, putative |
| similar to xyloglucan endotransglycosylase TCH4 GI: 886116 from [Arabidopsis thaliana] (68417.m04304) | |||||
| 199 | (C) Internal Glu Repression | D-NC-NC | 253631_at | At4g30440 | NAD-dependent epimerase/dehydratase family protein similar to nucleotide sugar epimerase from Vibrio vulniticus GI: 3093975 |
| [PID: g3093975], WbnF [Escherichia coli] GI: 5739472, CAPI protein {Staphylococcus aureus} SP|P39858; contains Pfam profile | |||||
| PF01370 NAD dependent epimerase/dehydratase family (68417.m04323) | |||||
| 200 | (C) Internal Glu Repression | D-NC-NC | 253600_at | At4g30810 | serine carboxypeptidase S10 family protein similar to serine-type carboxypeptidase (SP: P55748) [Hordeum vulgare] |
| (68417.m04365) | |||||
| 201 | (C) Internal Glu Repression | D-NC-NC | 253551_at | At4g30996 | expressed protein (68417.m04401) |
| 202 | (C) Internal Glu Repression | D-NC-NC | 253485_at | At4g31800 | WRKY family transcription factor (68417.m04517) |
| 203 | (C) Internal Glu Repression | D-NC-NC | 253377_at | At4g33300 | disease resistance protein (CC-NBS-LRR class), putative domain signature CC-NBS-LRR exists, suggestive of a disease |
| resistance protein. (68417.m04737) | |||||
| 204 | (C) Internal Glu Repression | D-NC-NC | 253357_at | At4g33400 | dem protein-related/defective embryo and meristems protein-related identical to dem GI: 2190419 from [Lycopersicon esculentum] |
| (68417.m04747) | |||||
| 205 | (C) Internal Glu Repression | D-NC-NC | 253351_at | At4g33700 | CBS domain-containing protein contains Pfam profiles PF00571: CBS domain, PF01595: Domain of unknown function |
| (68417.m04786) | |||||
| 206 | (C) Internal Glu Repression | D-NC-NC | 253300_at | At4g33580 | carbonic anhydrase family protein/carbonate dehydratase family protein similar to SP|P46512 Carbonic anhydrase 1 (EC 4.2.1.1) |
| (Carbonate dehydratase 1) {Flaveria linearis}; contains Pfam profile PF00484: Carbonic anhydrase (68417.m04771) | |||||
| 207 | (C) Internal Glu Repression | D-NC-NC | 253308_at | At4g33680 | aminotransferase class I and II family protein low similarity to Aromatic Aminotransferase from Pyrococcus horikoshii GP|14278621; |
| contains Pfam profile PF00155 aminotransferase, classes I and II (68417.m04784) | |||||
| 208 | (C) Internal Glu Repression | D-NC-NC | 253276_at | At4g34050 | caffeoyl-CoA 3-O-methyltransferase, putative nearly identical to GI: 2960356 [Populus balsamifera subsp. trichocarpa], GI: 684942 |
| [Medicago sativa subsp. sativa] isoform contains a GT-TG intron which removes an internal segment of the protein. | |||||
| (68417.m04831) | |||||
| 209 | (C) Internal Glu Repression | D-NC-NC | 253231_at | At4g34450 | coatomer gamma-2 subunit, putative/gamma-2 coat protein, putative/gamma-2 COP, putative similar to SP|Q9UBF2 Coatomer |
| gamma-2 subunit (Gamma-2 coat protein) (Gamma-2 COP) {Homo sapiens}; contains Pfam profile: PF01602 Adaptin N terminal | |||||
| region (68417.m04896) | |||||
| 210 | (C) Internal Glu Repression | D-NC-NC | 253083_at | At4g36250 | aldehyde dehydrogenase family protein contais aldehyde dehydrogenase (NADP) family protein domain, Pfam: PF00171 |
| (68417.m05156) | |||||
| 211 | (C) Internal Glu Repression | D-NC-NC | 253024_at | At4g38080 | hydroxyproline-rich glycoprotein family protein contains proline-rich extensin domains, INTERPRO: IPR002965; Common family |
| member; At2g22510 [Arabidopsis thaliana] (68417.m05378) | |||||
| 212 | (C) Internal Glu Repression | D-NC-NC | 252921_at | At4g39030 | enhanced disease susceptibility 5 (EDS5)/salicylic acid induction deficient 1 (SID1) identical to SP|Q945F0; contains Pfam profile |
| PF01554: Uncharacterized membrane protein family (68417.m05528) | |||||
| 213 | (C) Internal Glu Repression | D-NC-NC | 252932_at | At4g39080 | vacuolar proton ATPase, putative similar to Swiss-Prot: Q93050 vacuolar proton translocating ATPase 116 kDa subunit A isoform 1 |
| (Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit, Vacuolar proton pump subunit 1, Vacuolar adenosine | |||||
| triphosphatase subunit Ac116) [Homo sapiens] (68417.m05534) | |||||
| 214 | (C) Internal Glu Repression | D-NC-NC | 252943_at | At4g39330 | mannitol dehydrogenase, putative nearly identical to SP|P42734, probable mannitol dehydrogenase (68417.m05568) |
| 215 | (C) Internal Glu Repression | D-NC-NC | 252863_at | At4g39800 | inositol-3-phosphate synthase isozyme 1/myo-inositol-1-phosphate synthase 1/MI-1-P synthase 1/IPS 1 identical to SP|P42801 |
| Inositol-3-phosphate synthase isozyme 1 (EC 5.5.1.4) (Myo-inositol-1-phosphate synthase 1) (MI-1-P synthase 1) (IPS 1) | |||||
| {Arabidopsis thaliana} (68417.m05637) | |||||
| 216 | (C) Internal Glu Repression | D-NC-NC | 252652_at | At3g44720 | prephenate dehydratase family protein similar to bacterial PheA gene products (68416.m04813) |
| 217 | (C) Internal Glu Repression | D-NC-NC | 252377_at | At3g47960 | proton-dependent oligopeptide transport (POT) family protein contains Pfam profile; PF00854 POT family (68416.m05229) |
| 218 | (C) Internal Glu Repression | D-NC-NC | 252343_at | At3g48610 | phosphoesterase family protein low similarity to SP|P95246 Phospholipase C 2 precursor (EC 3.1.4.3) {Mycobacterium |
| tuberculosis}; contains Pfam profile PF04185: Phosphoesterase family (68416.m05307) | |||||
| 219 | (C) Internal Glu Repression | D-NC-NC | 252325_at | At3g48560 | acetolactate synthase, chloroplast/acetohydroxy-acid synthase (ALS) nearly identical to SP|P17597 Acetolactate synthase, |
| chloroplast precursor (EC 2.2.1.6, formerly EC 4.1.3.18) (Acetohydroxy-acid synthase) (ALS) {Arabidopsis thaliana} | |||||
| (68416.m05302) | |||||
| 220 | (C) Internal Glu Repression | D-NC-NC | 252272_at | At3g49670 | leucine-rich repeat transmembrane protein kinase, putative CLAVATA1 receptor kinase, Arabidopsis thaliana, EMBL: ATU96879 |
| (68416.m05429) | |||||
| 221 | (C) Internal Glu Repression | D-NC-NC | 252126_at | At3g50950 | disease resistance protein (CC-NBS-LRR class), putative domain signature CC-NBS-LRR exists, suggestive of a disease |
| resistance protein. (68416.m05578) | |||||
| 222 | (C) Internal Glu Repression | D-NC-NC | 252045_at | At3g52450 | U-box domain-containing protein similar to immediate-early fungal elicitor protein CMPG1 [Petroselinum crispum] GI: 14582200; |
| contains Pfam profile PF04564: U-box domain (68416.m05768) | |||||
| 223 | (C) Internal Glu Repression | D-NC-NC | 252058_at | At3g52470 | harpin-induced family protein/HIN1 family protein/harpin-responsive family protein similar to harpin-induced protein hin1 |
| (GI: 1619321) [Nicotiana tabacum] (68416.m05770) | |||||
| 224 | (C) Internal Glu Repression | D-NC-NC | 251954_at | At3g53670 | expressed protein (68416.m05927) |
| 225 | (C) Internal Glu Repression | D-NC-NC | 251922_at | At3g54030 | protein kinase family protein contains protein kinase domain, Pfam: PF00069 (68416.m05974) |
| 226 | (C) Internal Glu Repression | D-NC-NC | 251855_at | At3g54690 | sugar isomerase (SIS) domain-containing protein/CBS domain-containing protein similar to SP|Q47334 Polysialic acid capsule |
| expression protein kpsF {Escherichia coli}; contains Pfam profiles PF01380: sugar isomerase (SIS) domain, PF00571: CBS domain | |||||
| (68416.m06051) | |||||
| 227 | (C) Internal Glu Repression | D-NC-NC | 251828_at | At3g55070 | expressed protein (68416.m06116) |
| 228 | (C) Internal Glu Repression | D-NC-NC | 251449_at | At3g59920 | Rab GDP dissociation inhibitor (GDI2) identical to Rab GDP dissociation inhibitor AtGDI2 [Arabidopsis thaliana] GI: 2446981 |
| (68416.m06687) | |||||
| 229 | (C) Internal Glu Repression | D-NC-NC | 251450_at | At3g60030 | squamosa promoter-binding protein-like 12 (SPL12) identical to squamosa promoter binding protein-like 12 [Arabidopsis thaliana] |
| GI: 6006395; contains Pfam profiles PF03110: SBP domain, PF00023: Ankyrin repeat (68416.m06704) | |||||
| 230 | (C) Internal Glu Repression | D-NC-NC | 251396_at | At3g60750 | transketolase, putative strong similarity to transketolase 1 [Capsicum annuum] GI: 3559814; contains Pfam profiles PF02779: |
| Transketolase, pyridine binding domain, PF02780: Transketolase, C-terminal domain, PF00456: Transketolase, thiamine | |||||
| diphosphate binding domain (68416.m06796) | |||||
| 231 | (C) Internal Glu Repression | D-NC-NC | 251310_at | At3g61150 | homeobox-leucine zipper family protein/homeodomain GLABRA2 like protein 1 (HD-GL2-1) similar to Anthocyaninless2 (ANL2) |
| (GP: 5702094) Arabidopsis thaliana. EMBL: AF077335 (68416.m06843) | |||||
| 232 | (C) Internal Glu Repression | D-NC-NC | 251200_at | At3g63010 | expressed protein similar to PrMC3 [Pinus radiata] GI: 5487873 (68416.m07078) |
| 233 | (C) Internal Glu Repression | D-NC-NC | 251122_at | At5g01020 | protein kinase family protein contains protein kinase domain, Pfam: PF00069 (68418.m00004) |
| 234 | (C) Internal Glu Repression | D-NC-NC | 251060_at | At5g01820 | CBL-interacting protein kinase 14 (CIPK14) identical to CBL-interacting protein kinase 14 [Arabidopsis thaliana] |
| gi|13249127|gb|AAK16689; contains Pfam profiles PF00069: Protein kinase domain and PF03822: NAF domain; identical to cDNA | |||||
| CBL-interacting protein kinase 14 (CIPK14) GI: 13249126 (68418.m00101) | |||||
| 235 | (C) Internal Glu Repression | D-NC-NC | 251074_at | At5g01800 | saposin B domain-containing protein contains Pfam profiles: PF00026 eukaryotic aspartyl protease, PF03489 surfactant protein B, |
| PF05184 saposin-like type B, region 1 (68418.m00099) | |||||
| 236 | (C) Internal Glu Repression | D-NC-NC | 251020_at | At5g02270 | ABC transporter family protein NBD-like protein POP, Arabidopsis thaliana, EMBL: AF127664 (68418.m00150) |
| 237 | (C) Internal Glu Repression | D-NC-NC | 250926_at | At5g03555 | permease, cytosine/purines, uracil, thiamine, allantoin family protein contains Pfam PF02133: permease, cytosine/purines, uracil, |
| thiamine, allantoin family (68418.m00313) | |||||
| 238 | (C) Internal Glu Repression | D-NC-NC | 250738_at | At5g05730 | anthranilate synthase, alpha subunit, component I-1 (ASA1) identical to SP|P32068 (68418.m00630) |
| 239 | (C) Internal Glu Repression | D-NC-NC | 250645_at | At5g06700 | expressed protein strong similarity to unknown protein (emb|CAB82953.1) (68418.m00757) |
| 240 | (C) Internal Glu Repression | D-NC-NC | 250193_at | At5g14540 | proline-rich family protein contains proline rich extensin domains, INTERPRO: IPR002965 (68418.m01704) |
| 241 | (C) Internal Glu Repression | D-NC-NC | 250054_at | At5g17860 | cation exchanger, putative (CAX7) contains similarity to SWISS-PROT: Q9HC58 NKX3_HUMAN Sodium/potassium/calcium |
| exchanger 3 precursor {Homo sapiens}; Ca2+: Cation Antiporter (CaCA) Family member PMID: 11500563 (68418.m02093) | |||||
| 242 | (C) Internal Glu Repression | D-NC-NC | 249627_at | At5g37510 | NADH-ubiquinone dehydrogenase, mitochondrial, putative similar to NADH-ubiquinone oxidoreductase 75 kDa subunit, |
| mitochondrial from Solanum tuberosum [SP|Q43644] (68418.m04517) | |||||
| 243 | (C) Internal Glu Repression | D-NC-NC | 249599_at | At5g37990 | S-adenosyl-L-methionine:carboxyl methyltransferase family protein similar to defense-related protein cjs1 [Brassica |
| carinata][GI: 14009292], caffeine synthase [Camellia sinensis][GI: 9967143], SAM: jasmonic acid carboxyl methyltransferase | |||||
| [GI: 13676829] (68418.m04575) | |||||
| 244 | (C) Internal Glu Repression | D-NC-NC | 249244_at | At5g42270 | FtsH protease, putative similar to FtsH protease GI: 13183728 from [Medicago sativa] (68418.m05145) |
| 245 | (C) Internal Glu Repression | D-NC-NC | 249247_at | At5g42310 | pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat (68418.m05149) |
| 246 | (C) Internal Glu Repression | D-NC-NC | 249152_s_at | At5g43370 | inorganic phosphate transporter (PHT2) identical to inorganic phosphate transporter [Arabidopsis thaliana] GI: 2780348 |
| (68418.m05301) | |||||
| 247 | (C) Internal Glu Repression | D-NC-NC | 248968_at | At5g45280 | pectinacetylesterase, putative similar to pectinacetylesterase precursor GI: 1431629 from [Vigna radiata] (68418.m05558) |
| 248 | (C) Internal Glu Repression | D-NC-NC | 248697_at | At5g48370 | thioesterase family protein similar to SP|Q9R0X4 48 kDa acyl-CoA thioester hydrolase, mitochondrial precursor (EC 3.1.2.—) {Mus |
| musculus}; contains Pfam profile PF03061: thioesterase family protein (68418.m05976) | |||||
| 249 | (C) Internal Glu Repression | D-NC-NC | 248619_at | At5g49630 | amino acid permease 6 (AAP6) identical to amino acid permease 6 (AAP6) [Arabidopsis thaliana] GI: 1769887 (68418.m06141) |
| 250 | (C) Internal Glu Repression | D-NC-NC | 248573_at | At5g49720 | endo-1,4-beta-glucanase KORRIGAN (KOR)/cellulase (OR16pep) identical to endo-1,4-beta-D-glucanase KORRIGAN |
| [Arabidopsis thaliana] GI: 3978258; similar to endo-1,4-beta-D-glucanase; cellulase GI: 5689613 from [Brassica napus]; identical to | |||||
| cDNA cellulase (OR16pep) GI: 1022806 (68418.m06157) | |||||
| 251 | (C) Internal Glu Repression | D-NC-NC | 248263_at | At5g53370 | pectinesterase family protein (68418.m06632) |
| 252 | (C) Internal Glu Repression | D-NC-NC | 248200_at | At5g54160 | quercetin 3-O-methyltransferase 1/flavonol 3-O-methyltransferase 1/caffeic acid/5-hydroxyferulic acid O-methyltransferase |
| (OMT1) identical to O-methyltransferase 1 [Arabidopsis thaliana][GI: 2781394], SP|Q9FK25 Quercatin 3-O-methyltransferase 1 (EC | |||||
| 2.1.1.76) (AtOMT1) (Flavonol 3-O-methyltransferase 1) (Caffeic acid/5-hydroxyferulic acid O-methyltransferase) {Arabidopsis | |||||
| thaliana} (68418.m06744) | |||||
| 253 | (C) Internal Glu Repression | D-NC-NC | 248138_at | At5g54960 | pyruvate decarboxylase, putative strong similarity to pyruvate decarboxylase 1 [Vitis vinifera] GI: 10732644; contains InterPro entry |
| IPR000399: Pyruvate decarboxylase (68418.m06845) | |||||
| 254 | (C) Internal Glu Repression | D-NC-NC | 248034_at | At5g55910 | protein kinase, putative contains protein kinase domain, Pfam: PF00069 (68418.m06972) |
| 255 | (C) Internal Glu Repression | D-NC-NC | 248009_at | At5g56280 | COP9 signalosome subunit 6/CSN subunit 6 (CSN6A) identical to CSN complex subunit 6A [Arabidopsis thaliana] GI: 18056665, |
| COP9 complex subunit 6 [Arabidopsis thaliana] GI: 15809663; contains Pfam profile PF01398: Mov34/MPN/PAD-1 family; identical | |||||
| to cDNA CSN complex subunit 6A (CSN6A) GI: 18056664 (68418.m07024) | |||||
| 256 | (C) Internal Glu Repression | D-NC-NC | 247853_at | At5g58140 | protein kinase family protein/non phototropic hypocotyl 1-like protein (NPL1) contains Pfam domains, PF00069: Protein kinase |
| domain and PF00785: PAC motif; similar to SP: O48963 Nonphototropic hypocotyl protein 1 (Phototropin) [Mouse-ear cress] | |||||
| {Arabidopsis thaliana}; identical to cDNA non phototropic hypocotyl 1-like (NPL1) GI: 5391441 (68418.m07274) | |||||
| 257 | (C) Internal Glu Repression | D-NC-NC | 247653_at | At5g59950 | RNA and export factor-binding protein, putative (68418.m07517) |
| 258 | (C) Internal Glu Repression | D-NC-NC | 247601_at | At5g60850 | Dof-type zinc finger domain-containing protein similar to zinc finger protein OBP4 gi: 5059396 from [Arabidopsis thaliana]; |
| EMBL: AF155817 (68418.m07633) | |||||
| 259 | (C) Internal Glu Repression | D-NC-NC | 247304_at | At5g63850 | amino acid transporter 4, putative (AAP4) identical to amino acid transporter GI: 608671 from [Arabidopsis thaliana]; |
| (68418.m08015) | |||||
| 260 | (C) Internal Glu Repression | D-NC-NC | 247262_at | At5g64440 | amidase family protein low similarity to enantiomerase-selective amidase [Rhodococcus sp.] GI: 152052; contains Pfam profile |
| PF01425: Amidase (68418.m08095) | |||||
| 261 | (C) Internal Glu Repression | D-NC-NC | 247152_at | At5g65620 | peptidase M3 family protein/thimet oligopeptidase family protein similar to SP|P27237 Oligopeptidase A (EC 3.4.24.70) |
| {Salmonella typhimurium}; contains Pfam profile PF01432: Peptidase family M3 (68418.m08255) | |||||
| 262 | (C) Internal Glu Repression | D-NC-NC | 246990_at | At5g67360 | cucumisin-like serine protease (ARA12) Asp48; almost identical to cucumisin-like serine protease (ARA12) GI: 3176874 from |
| [Arabidopsis thaliana] (68418.m08494) | |||||
| 263 | (C) Internal Glu Repression | D-NC-NC | 246901_at | At5g25630 | pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat (68418.m03050) |
| 264 | (C) Internal Glu Repression | D-NC-NC | 246825_at | At5g26260 | meprin and TRAF homology domain-containing protein/MATH domain-containing protein similar to ubiquitin-specific protease 12 |
| [Arabidopsis thaliana] GI: 11993471; contains Pfam profile PF00917: MATH domain (68418.m03133) | |||||
| 265 | (C) Internal Glu Repression | D-NC-NC | 246651_at | At5g35170 | adenylate kinase family protein contains Pfam profile: PF00406 adenylate kinase (68418.m04168) |
| 266 | (C) Internal Glu Repression | D-NC-NC | 246660_at | At5g35180 | expressed protein (68418.m04169) |
| 267 | (C) Internal Glu Repression | D-NC-NC | 246554_at | At5g15450 | heat shock protein 100, putative/HSP100, putative/heat shock protein clpB, putative/HSP100/ClpB, putative similar to |
| HSP100/ClpB GI: 9651530 [Phaseolus lunatus] (68418.m01808) | |||||
| 268 | (C) Internal Glu Repression | D-NC-NC | 246366_at | At1g51850 | leucine-rich repeat protein kinase, putative similar to light repressible receptor protein kinase [Arabidopsis thaliana] |
| gi|1321686|emb|CAA66376 (68414.m05845) | |||||
| 269 | (C) Internal Glu Repression | D-NC-NC | 246228_at | At4g36430 | peroxidase, putative identical to peroxidase [Arabidopsis thaliana] gi|6822093|emb|CAB71009; identical to cDNA class III |
| peroxidase ATP31, GI: 17530561 (68417.m05175) | |||||
| 270 | (C) Internal Glu Repression | D-NC-NC | 246247_at | At4g36640 | SEC14 cytosolic factor family protein/phosphoglyceride transfer family protein similar to polyphosphoinositide binding protein |
| Ssh1p (GI: 2739044) {Glycine max, SEC14 cytosolic factor (Phosphatidylinositol/phosphatidylcholine transfer protein) (PI/PCTP) | |||||
| (SP: P24859) [Kluyveromyces lactis] and to SEC14 cytosolic factor (SP: P53989) [Candida glabrata] (68417.m05200) | |||||
| 271 | (C) Internal Glu Repression | D-NC-NC | 246185_at | At5g20980 | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase, putative/vitamin-B12-independent methionine synthase, |
| putative/cobalamin-independent methionine synthase, putative strong similarity to SP|O50008 5- | |||||
| methyltetrahydropteroyltriglutamate-homocysteine methyltransferase (EC 2.1.1.14) (Vitamin-B12-independent methionine | |||||
| synthase isozyme) (Cobalamin-independent methionine synthase isozyme) {Arabidopsis thaliana}; contains Pfam profile PF01717: | |||||
| Methionine synthase, vitamin-B12 independent (68418.m02494) | |||||
| 272 | (C) Internal Glu Repression | D-NC-NC | 246126_at | At5g20070 | MutT/nudix family protein low similarity to SP|Q19427 NADH pyrophosphatase (EC 3.6.1.—) (Caenorhabditis elegans); contains |
| Pfam profile PF00293: NUDIX domain (68418.m02390) | |||||
| 273 | (C) Internal Glu Repression | D-NC-NC | 246050_s_at | At5g28900 | calcium-binding EF hand family protein contains Pfam profile: PF00036 EF hand (68418.m03562) |
| 274 | (C) Internal Glu Repression | D-NC-NC | 245971_at | At5g20730 | auxin-responsive factor (ARF7) identical to auxin response factor 7 GI: 4104929 from [Arabidopsis thaliana] (68418.m02462) |
| 275 | (C) Internal Glu Repression | D-NC-NC | 245885_at | At5g09440 | phosphate-responsive protein, putative similar to phi-1 (phosphate-induced gene) [Nicotiana tabacum] GI: 3759184; contains Pfam |
| profile PF04674: Phosphate-induced protein 1 conserved region (68418.m01093) | |||||
| 276 | (C) Internal Glu Repression | D-NC-NC | 245875_at | At1g26240 | proline-rich extensin-like family protein similar to hydroxyproline-rich glycoprotein precursor gi|727264|gb|AAA87902; contains |
| proline-rich extensin domains, INTERPRO: IPR002965 (68414.m03201) | |||||
| 277 | (C) Internal Glu Repression | D-NC-NC | 245803_at | At1g47128 | cysteine proteinase (RD21A)/thiol protease identical to SP|P43297 Cysteine proteinase RD21A precursor (EC 3.4.22.—) |
| {Arabidopsis thaliana}, thiol protease RD21A SP: P43297 from [Arabidopsis thaliana] (68414.m05222) | |||||
| 278 | (C) Internal Glu Repression | D-NC-NC | 245780_at | At1g45688 | expressed protein (68414.m05201) |
| 279 | (C) Internal Glu Repression | D-NC-NC | 245759_at | At1g66900 | expressed protein (68414.m07603) |
| 280 | (C) Internal Glu Repression | D-NC-NC | 245612_at | At4g14440 | enoyl-CoA hydratase/isomerase family protein low similarity to PhaB [Pseudomonas putida] GI: 3253198, SP|P31551 Carnitine |
| racemase {Escherichia coli}; contains Pfam profile PF00378 enoyl-CoA hydratase/isomerase family protein (68417.m02227) | |||||
| 281 | (C) Internal Glu Repression | D-NC-NC | 245270_at | At4g14960 | tubulin alpha-6 chain (TUA6) nearly identical to SP|P29511 Tubulin alpha-6 chain {Arabidopsis thaliana} (68417.m02298) |
| 282 | (C) Internal Glu Repression | D-NC-NC | 245218_s_at | At1g58842 | |
| 283 | (C) Internal Glu Repression | D-NC-NC | 245201_at | At1g67840 | ATP-binding region, ATPase-like domain-containing protein contains Pfam profile PF02518: ATPase, histidine kinase-, DNA gyrase |
| B-, and HSP90-like domain protein; similar to ESTs gb|AI995396.1, dbj|AV557393.1, and dbj|AV557055.1 (68414.m07743) | |||||
| 284 | (C) Internal Glu Repression | D-NC-NC | 245211_at | At5g12370 | exocyst complex component Sec10-related low similarity to SP|O00471 Exocyst complex component Sec10 (hSec10) {Homo |
| sapiens} (68418.m01455) | |||||
| 285 | (C) Internal Glu Repression | D-NC-NC | 245101_at | At2g40890 | cytochrome P450 98A3, putative (CYP98A3) identical to Cytochrome P450 98A3 (SP|O22203) [Arabidopsis thaliana]; similar to |
| gi: 17978651 from Pinus taeda (68415.m05046) | |||||
| 286 | (C) Internal Glu Repression | NC-I-I | 267451_at | At2g33710 | AP2 domain-containing transcription factor family protein similar to RAP2.6 (GI: 17065542) (Arabidopsis thaliana) (68415.m04132) |
| 287 | (C) Internal Glu Repression | NC-I-I | 267246_at | At2g30250 | WRKY family transcription factor (68415.m03682) |
| 288 | (C) Internal Glu Repression | NC-I-I | 267261_at | At2g23120 | expressed protein (68415.m02758) |
| 289 | (C) Internal Glu Repression | NC-I-I | 267083_at | At2g41100 | touch-responsive protein/calmodulin-related protein 3, touch-induced (TCH3) identical to calmodulin-related protein 3, touch- |
| induced SP: P25071 from [Arabidopsis thaliana] (68415.m05076) | |||||
| 290 | (C) Internal Glu Repression | NC-I-I | 266832_at | At2g30040 | protein kinase family protein contains protein kinase domain, Pfam: PF00069 (68415.m03653) |
| 291 | (C) Internal Glu Repression | NC-I-I | 266749_at | At2g47060 | serine/threonine protein kinase, putative similar to Pto kinase interactor 1 (Pti1)[Lycopersicon esculentum] |
| gi|3668069|gb|AAC61805 (68415.m05879) | |||||
| 292 | (C) Internal Glu Repression | NC-I-I | 266299_at | At2g29450 | glutathione S-transferase (103-1A) identical to Swiss-Prot: P46421 glutathione S-transferase 103-1A [Arabidopsis thaliana] |
| (68415.m03578) | |||||
| 293 | (C) Internal Glu Repression | NC-I-I | 266172_at | At2g39010 | aquaporin, putative similar to plasma membrane aquaporin 2b GI: 7209560 from [Raphanus sativus] (68415.m04796) |
| 294 | (C) Internal Glu Repression | NC-I-I | 266010_at | At2g37430 | zinc finger (C2H2 type) family protein (ZAT11) contains Pfam domain, PF00096: Zinc finger, C2H2 type (68415.m04590) |
| 295 | (C) Internal Glu Repression | NC-I-I | 265670_s_at | At2g32210 | expressed protein (68415.m03936) |
| 296 | (C) Internal Glu Repression | NC-I-I | 265480_at | At2g15970 | cold-acclimation protein, putative (FL3-5A3) similar to cold acclimation WCOR413-like protein gamma form [Hordeum vulgare] |
| gi|18449100|gb|AAL69988; similar to stress-regulated protein SAP1 [Xerophyta viscosa] gi|21360376|gb|AAM47505; identical to | |||||
| cDNA cold acclimation protein WCOR413-like protein alpha form GI: 10121840, cold acclimation protein homolog [Arabidopsis | |||||
| thaliana] GI: 11127595 (68415.m01828) | |||||
| 297 | (C) Internal Glu Repression | NC-I-I | 265460_at | At2g46600 | calcium-binding protein, putative similar to EF-hand Ca2+-binding protein CCD1 [Triticum aestivum] GI: 9255753 (68415.m05812) |
| 298 | (C) Internal Glu Repression | NC-I-I | 264652_at | At1g08920 | sugar transporter, putative similar to ERD6 protein {Arabidopsis thaliana} GI: 3123712, sugar-porter family proteins 1 and 2 |
| [Arabidopsis thaliana] GI: 14585699, GI: 14585701; contains Pfam profile PF00083: major facilitator superfamily protein | |||||
| (68414.m00992) | |||||
| 299 | (C) Internal Glu Repression | NC-I-I | 263478_at | At2g31880 | leucine-rich repeat transmembrane protein kinase, putative (68415.m03895) |
| 300 | (C) Internal Glu Repression | NC-I-I | 263379_at | At2g40140 | zinc finger (CCCH-type) family protein contains Pfam domain, PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and similar) and Pfam |
| domain, PF00023: Ankyrin repeat (68415.m04937) | |||||
| 301 | (C) Internal Glu Repression | NC-I-I | 263320_at | At2g47180 | galactinol synthase, putative similar to galactinol synthase, isoform GoIS-1 GI: 5608497 from [Ajuga reptans] (68415.m05892) |
| 302 | (C) Internal Glu Repression | NC-I-I | 262360_at | At1g73080 | leucine-rich repeat transmembrane protein kinase, putative similar to receptor protein kinase GI: 1389566 from [Arabidopsis |
| thaliana] (68414.m08450) | |||||
| 303 | (C) Internal Glu Repression | NC-I-I | 261979_at | At1g37130 | nitrate reductase 2 (NR2) identical to SP|P11035 Nitrate reductase 2 (formerly EC 1.6.6.1) (NR2) {Arabidopsis thaliana} |
| (68414.m04639) | |||||
| 304 | (C) Internal Glu Repression | NC-I-I | 259681_at | At1g77760 | nitrate reductase 1 (NR1) identical to SP|P11832 Nitrate reductase 1 (formerly EC 1.6.6.1) (NR1){Arabidopsis thaliana} |
| (68414.m09053) | |||||
| 305 | (C) Internal Glu Repression | NC-I-I | 259445_at | At1g02400 | gibberellin 2-oxidase, putative/GA2-oxidase, putative similar to GA2ox2 [GI: 4678368]; similar to dioxygenase GI: 1666096 from |
| [Marah macrocarpus]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain (68414.m00186) | |||||
| 306 | (C) Internal Glu Repression | NC-I-I | 258436_at | At3g16720 | zinc finger (C3HC4-type RING finger) family protein contains Pfam domain, PF00097: Zinc finger, C3HC4 type (RING finger) |
| (68416.m02135) | |||||
| 307 | (C) Internal Glu Repression | NC-I-I | 258362_at | At3g14280 | expressed protein (68416.m01807) |
| 308 | (C) Internal Glu Repression | NC-I-I | 256526_at | At1g66090 | disease resistance protein (TIR-NBS class), putative domain signature TIR-NBS exists, suggestive of a disease resistance protein. |
| (68414.m07501) | |||||
| 309 | (C) Internal Glu Repression | NC-I-I | 256017_at | At1g19180 | expressed protein (68414.m02387) |
| 310 | (C) Internal Glu Repression | NC-I-I | 255568_at | At4g01250 | WRKY family transcription factor contains Pfam profile: PF03106 WRKY DNA-binding domain (68417.m00164) |
| 311 | (C) Internal Glu Repression | NC-I-I | 253643_at | At4g29780 | expressed protein (68417.m04241) |
| 312 | (C) Internal Glu Repression | NC-I-I | 253638_at | At4g30470 | cinnamoyl-CoA reductase-related similar to cinnamoyl-CoA reductase from Pinus taeda [GI: 17978649], Saccharum officinarum |
| [GI: 3341511] (68417.m04326) | |||||
| 313 | (C) Internal Glu Repression | NC-I-I | 253414_at | At4g33050 | calmodulin-binding family protein contains Pfam profile PF00612: IQ calmodulin-binding motif (68417.m04703) |
| 314 | (C) Internal Glu Repression | NC-I-I | 253284_at | At4g34150 | C2 domain-containing protein similar to calcium-dependent protein kinase [Dunaliella tertiolecta] GI: 6644464; contains Pfam profile |
| PF00168: C2 domain (68417.m04846) | |||||
| 315 | (C) Internal Glu Repression | NC-I-I | 252906_at | At4g39640 | gamma-glutamyltranspeptidase family protein similar to SP|P19440 Gamma-glutamyltranspeptidase 1 precursor (EC 2.3.2.2) |
| (Gamma-glutamyltransferase 1) (CD224 antigen) {Homo sapiens}; contains Pfam profilePF01019: Gamma-glutamyltranspeptidase | |||||
| (68417.m05602) | |||||
| 316 | (C) Internal Glu Repression | NC-I-I | 252592_at | At3g45640 | mitogen-activated protein kinase, putative/MAPK, putative (MPK3) identical to mitogen-activated protein kinase homolog |
| (AtMPK3)[Arabidopsis thaliana] SWISS-PROT: Q39023; PMID: 12119167 (68416.m04929) | |||||
| 317 | (C) Internal Glu Repression | NC-I-I | 252474_at | At3g46620 | zinc finger (C3HC4-type RING finger) family protein contains Pfam domain, PF00097: Zinc finger, C3HC4 type (RING finger) |
| (68416.m05061) | |||||
| 318 | (C) Internal Glu Repression | NC-I-I | 252483_at | At3g46600 | scarecrow transcription factor family protein scarecrow-like 11 - Arabidopsis thaliana, EMBL: AF036307 (68416.m05058) |
| 319 | (C) Internal Glu Repression | NC-I-I | 252315_at | At3g48690 | expressed protein similar to PrMC3 [Pinus radiata] GI: 5487873 (68416.m05317) |
| 320 | (C) Internal Glu Repression | NC-I-I | 251336_at | At3g61190 | BON1-associated protein 1 (BAP1) identical to BON1-associated protein 1 [Arabidopsis thaliana] GI: 15487384; contains Pfam |
| profile PF00168: C2 domain; supporting cDNA gi|15487383|gb|AY045765.1| (68416.m06848) | |||||
| 321 | (C) Internal Glu Repression | NC-I-I | 251272_at | At3g61890 | homeobox-leucine zipper protein 12 (HB-12)/HD-ZIP transcription factor 12 identical to homeobox-leucine zipper protein ATHB-12 |
| (GI: 6899887) [Arabidopsis thaliana] (68416.m06951) | |||||
| 322 | (C) Internal Glu Repression | NC-I-I | 251054_at | At5g01540 | lectin protein kinase, putative similar to receptor lectin kinase 3 [Arabidopsis thaliana] gi|4100060|gb|AAD00733; contains protein |
| kinase domain, Pfam: PF00069: contains legume lectins alpha and beta domains, Pfam: PF00138 and Pfam: PF00139 | |||||
| (68418.m00069) | |||||
| 323 | (C) Internal Glu Repression | NC-I-I | 250676_at | At5g06320 | harpin-induced family protein/HIN1 family protein/harpin-responsive family protein/NDR1/HIN1-like protein 3 similar to harpin- |
| induced protein hin1 (GI: 1819321)[Nicotiana tabacum] (68418.m00708) | |||||
| 324 | (C) Internal Glu Repression | NC-I-I | 250541_at | At5g09520 | hydroxyproline-rich glycoprotein family protein contains proline-rich extensin domains. INTERPRO: IPR002965 (68418.m01101) |
| 325 | (C) Internal Glu Repression | NC-I-I | 249918_at | At5g19240 | expressed protein (68418.m02291) |
| 326 | (C) Internal Glu Repression | NC-I-I | 249237_at | At5g42050 | expressed protein similar to gda-1 [Pisum sativum] GI: 2765418 (68418.m05119) |
| 327 | (C) Internal Glu Repression | NC-I-I | 247708_at | At5g59550 | zinc finger (C3HC4-type RING finger) family protein contains Pfam domain, PF00097: Zinc finger, C3HC4 type (RING finger) |
| (68418.m07462) | |||||
| 328 | (C) Internal Glu Repression | NC-I-I | 247279_at | At5g64310 | arabinogalactan-protein (AGP1) identical to gi: 3883120 gb: AAC77823 (68418.m08078) |
| 329 | (C) Internal Glu Repression | NC-I-I | 246962_s_at | At5g24800 | bZIP transcription factor family protein contains Pfam profile: PF00170 bZIP transcription factor; identical to cDNA bZIP protein |
| BZO2H2 GI: 10954096 (68418.m02928) | |||||
| 330 | (C) Internal Glu Repression | NC-I-I | 246821_at | At5g26920 | calmodulin-binding protein similar to calmodulin-binding protein TCB60 GI: 1698548 from [Nicotiana tabacum] (68418.m03210) |
| 331 | (C) Internal Glu Repression | NC-I-I | 246289_at | At3g56880 | VQ motif-containing protein contains PF05678: VQ motif (68416.m06327) |
| 332 | (C) Internal Glu Repression | NC-I-I | 245904_at | At5g11110 | sucrose-phosphate synthase, putative similar to sucrose-phosphate synthase isoform 1, Citrus unshiu, PIR: S72648 |
| (68418.m01297) | |||||
| 333 | (C) Internal Glu Repression | NC-I-I | 245051_at | At2g23320 | WRKY family transcription factor identical to WRKY DNA-binding protein 15 GI: 13506742 from [Arabidopsis thaliana] |
| (68415.m02784) | |||||
| 334 | (C) Internal Glu Repression | NC-I-I | 245041_at | At2g26530 | expressed protein (68415.m03183) |
| 1 | (D) External Glu induction | NC-NC-I | 266371_at | At2g41410 | calmodulin, putative identical to SP|P30188 Calmodulin-like protein {Arabidopsis thaliana} (68415.m05110) |
| 2 | (D) External Glu induction | NC-NC-I | 266116_at | At2g02180 | tobamovirus multiplication protein 3 (TOM3) identical to tobamovirus multiplication protein (TOM3) GI: 15425641 from [Arabidopsis |
| thaliana] (68415.m00154) | |||||
| 3 | (D) External Glu induction | NC-NC-I | 264415_at | At1g43160 | AP2 domain-containing protein RAP2.6 (RAP2.6) identical to AP2 domain containing protein RAP2.6 GI: 2281637 from [Arabidopsis |
| thaliana] (68414.m04973) | |||||
| 4 | (D) External Glu induction | NC-NC-I | 263901_at | At2g36320 | zinc finger (AN1-like) family protein contains Pfam domain, PF01428: AN1-like Zinc finger (68415.m04458) |
| 5 | (D) External Glu induction | NC-NC-I | 263228_at | At1g30700 | FAD-binding domain-containing protein similar to SP|P30986 reticuline oxidase precursor (Berberine-bridge-forming enzyme) (BBE) |
| (Tetrahydroprotoberberine synthase) [Eschscholzia californica]; contains PF01565 FAD binding domain (68414.m03753) | |||||
| 6 | (D) External Glu induction | NC-NC-I | 262901_at | At1g59910 | formin homology 2 domain-containing protein/FH2 domain-containing protein contains formin homology 2 domain, Pfam: PF02128 |
| (68414.m06749) | |||||
| 7 | (D) External Glu induction | NC-NC-I | 262887_at | At1g14780 | expressed protein (68414.m01767) |
| 8 | (D) External Glu induction | NC-NC-I | 262677_at | At1g75860 | expressed protein (68414.m08811) |
| 9 | (D) External Glu induction | NC-NC-I | 262496_at | At1g21790 | expressed protein (68414.m02727) |
| 10 | (D) External Glu induction | NC-NC-I | 262383_at | At1g72940 | disease resistance protein (TIR-NBS class), putative domain signature TIR-NBS exists, suggestive of a disease resistance protein. |
| (68414.m08436) | |||||
| 11 | (D) External Glu induction | NC-NC-I | 262166_at | At1g74840 | myb family transcription factor similar to myb-related transcription activator GI: 9279717 from [Arabidopsis thaliana] (68414.m08672) |
| 12 | (D) External Glu induction | NC-NC-I | 262133_at | At1g78000 | sulfate transporter (Sultr1;2) identical to sulfate transporter Sultr1;2 [Arabidopsis thaliana] GI: 7768660: contaisn Pfam profiles |
| PF00916: Sulfate transporter family and PF01740: STAS domain; contains TIGRfam profile TIGR00815: sulfate permease | |||||
| (68414.m09089) | |||||
| 13 | (D) External Glu induction | NC-NC-I | 261211_at | At1g12780 | UDP-glucose 4-epimerase/UDP-galactose 4-epimerase/Galactowaldenase identical to SP|Q42605 [GB: CAA90941] from |
| [Arabidopsis thaliana] (Arch. Biochem. Biophys. 327 (1), 27-34 (1996)) (68414.m01484) | |||||
| 14 | (D) External Glu induction | NC-NC-I | 260556_at | At2g43620 | chitinase, putative similar to basic endochitinase CHB4 precursor SP: Q06209 from [Brassica napus] (68415.m05422) |
| 15 | (D) External Glu induction | NC-NC-I | 259977_at | At1g76590 | zinc-binding family protein similar to zinc-binding protein [Pisum sativum] GI: 16117799; contains Pfam profile PF04640: Protein of |
| unknown function, DUF597 (68414.m08912) | |||||
| 16 | (D) External Glu induction | NC-NC-I | 258805_at | At3g04010 | glycosyl hydrolase family 17 protein similar to beta-1,3-glucanase GB: S12402 [Nicotiana sp], GB: CAA03908 [Citrus sinensis], |
| GB: S44364 [Lycopersicon esculentum] (68416.m00422) | |||||
| 17 | (D) External Glu induction | NC-NC-I | 258751_at | At3g05890 | hydrophobic protein (RCI2B)/low temperature and salt responsive protein (LTI6B) identical to SP|Q9ZNS6 Hydrophobic protein |
| RCI2B (Low temperature and salt responsive protein LTI6B) {Arabidopsis thaliana} (68416.m00662) | |||||
| 18 | (D) External Glu induction | NC-NC-I | 257087_at | At3g20500 | calcineurin-like phosphoesterase family protein contains Pfam profile: PF00149 calcineurin-like phosphoesterase (68416.m02596) |
| 19 | (D) External Glu induction | NC-NC-I | 256417_s_at | At3g11170 | omega-3 fatty acid desaturase, chloroplast (FAD7) (FADD) identical to omega-3 fatty acid desaturase, chloroplast precursor |
| SP: P46310 [Arabidopsis thaliana (Mouse-ear cress)]; identical to Pfam profile PF00487: Fatty acid desaturase; identical to cDNA | |||||
| plastid fatty acid desaturase GI: 809491 (68416.m01355) | |||||
| 20 | (D) External Glu induction | NC-NC-I | 256185_at | At1g51700 | Dof-type zinc finger domain-containing protein (ADOF1) identical to cDNA adof1 mRNA for dof zinc finger protein, GI: 3608260; |
| contains Pfam profile PF02701: Dof domain, zinc finger (68414.m05826) | |||||
| 21 | (D) External Glu induction | NC-NC-I | 254850_at | At4g12000 | expressed protein (68417.m01909) |
| 22 | (D) External Glu induction | NC-NC-I | 254667_at | At4g18280 | glycine-rich cell wall protein-related glycine-rich protein 1.0 precursor, Phaseolus vulgaris, PIR1: S01821 (68417.m02713) |
| 23 | (D) External Glu induction | NC-NC-I | 252368_at | At3g48520 | cytochrome P450 family protein similar to Cytochrome P450 94A1 (P450-dependent fatty acid omega-hydroxylase) (SP: O81117) |
| {Vicia sativa}; contains Pfam profile: PF00067 cytochrome P450 (68416.m05296) | |||||
| 24 | (D) External Glu induction | NC-NC-I | 252291_s_at | At3g49120 | peroxidase, putative identical to peroxidase [Arabidopsis thaliana] gi|405811|emb|CAA50677 (68416.m05366) |
| 25 | (D) External Glu induction | NC-NC-I | 252040_at | At3g52060 | expressed protein contains Pfam profile PF03267: Arabidopsis protein of unknown function, DUF266 (68416.m05711) |
| 26 | (D) External Glu induction | NC-NC-I | 250793_at | At5g05600 | oxidoreductase, 2OG-Fe(11) oxygenase family protein similar to flavonol synthase [Citrus unshiu][gi: 4126403], leucoanthocyanidin |
| dioxygenase [Daucus carota][gi: 5924383]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain (68418.m00609) | |||||
| 27 | (D) External Glu induction | NC-NC-I | 250260_at | At5g13810 | glutaredoxin family protein (68418.m01613) |
| 28 | (D) External Glu induction | NC-NC-I | 249889_at | At5g22540 | expressed protein contains Pfam profile PF03140: Plant protein of unknown function (68418.m02630) |
| 29 | (D) External Glu induction | NC-NC-I | 248870_at | At5g46710 | zinc-binding family protein similar zinc-binding protein [Pisum sativum] GI: 16117799; contains Pfam profile PF04640: Protein of |
| unknown function, DUF597 (68418.m05755) | |||||
| 30 | (D) External Glu induction | NC-NC-I | 248199_at | At5g54170 | expressed protein weak similarity to SP|Q9UKL6 Phosphatidylcholine transfer protein (PC-TP) {Homo sapiens} (68418.m06745) |
| 31 | (D) External Glu induction | NC-NC-I | 247706_at | At5g59480 | haloacid dehalogenase-like hydrolase family protein low similarity to SP|P53078 SSM1 protein {Saccharomyces cerevisiae}; |
| contains InterPro accession IPR005834: Haloacid dehalogenase-like hydrolase (68418.m07454) | |||||
| 32 | (D) External Glu induction | NC-NC-I | 246481_s_at | At5g15960 | stress-responsive protein (KIN1)/stress-induced protein (KIN1) identical to SP|P18612 Stress-induced KIN1 protein (Arabidopsis |
| thaliana) (68418.m01866) | |||||
| 33 | (D) External Glu induction | NC-NC-I | 246488_at | At5g16010 | 3-oxo-5-alpha-steroid 4-dehydrogenase family protein/steroid 5-alpha-reductase family protein similar to steroid 5alpha-Reductase |
| Rattus norvegicus, PIR: A34239 [SP|24008]; contains Pfam 3-oxo-5-alpha-steroid 4-dehydrogenase domain PF02544 | |||||
| (68418.m01872) | |||||
| 34 | (D) External Glu induction | NC-NC-I | 246253_at | At4g37260 | myb family transcription factor (MYB73) contains Pfam profile: PF00249 myb-like DNA-binding domain (68417.m05274) |
| 35 | (D) External Glu induction | NC-NC-I | 246097_at | At5g20270 | expressed protein contains Pfam domain, PF03006: Uncharacterised protein family (Hly-III/UPF0073) (68418.m02413) |
| 36 | (D) External Glu induction | NC-NC-I | 245781_at | At1g45976 | expressed protein (68414.m05206) |
| 37 | (D) External Glu induction | NC-NC-I | 245244_at | At1g44350 | IAA-amino acid hydrolase 6, putative (ILL6)/IAA-Ala hydrolase, putative virtually identical to gr1-protein from [Arabidopsis thaliana] |
| GI: 3559811; similar to IAA-amino acid hydrolase GI: 3421384 from [Arabidopsis thaliana]; contains TIGRfam profile TIGR01891: | |||||
| amidohydrolase; contains Pfam profile PF01546: Peptidase family M20/M25/M40; identical to cDNA IAA-amino acid conjugate | |||||
| hydrolase-like protein (ILL6), partial cds GI: 17978837 (68414.m05110) | |||||
| 38 | (D) External Glu repression | NC-NC-D | 267130_at | At2g23390 | expressed protein (68415.m02793) |
| 39 | (D) External Glu repression | NC-NC-D | 267050_at | At2g41060 | RNA recognition motif (RRM)-containing protein similar to UBP1 interacting protein 1a [Arabidopsis thaliana] GI: 19574236; contains |
| InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) (68415.m05070) | |||||
| 40 | (D) External Glu repression | NC-NC-D | 266957_at | At2g34640 | expressed protein (68415.m04255) |
| 41 | (D) External Glu repression | NC-NC-D | 266856_at | At2g26910 | ABC transporter family protein similar to PDR5-like ABC transporter GI: 1514643 from [Spirodela polyrhiza] (68415.m03228) |
| 42 | (D) External Glu repression | NC-NC-D | 266709_at | At2g03120 | signal peptide peptidase family protein contains Pfam domain PF04258: Membrane protein of unknown function (DUF435) |
| (68415.m00265) | |||||
| 43 | (D) External Glu repression | NC-NC-D | 266395_at | At2g43100 | aconitase C-terminal domain-containing protein contains Pfam profile PF00694: Aconitase C-terminal domain (68415.m05350) |
| 44 | (D) External Glu repression | NC-NC-D | 266215_at | At2g06850 | xyloglucan:xyloglucosyl transferase/xyloglucan endotransglycosylase/endo-xyloglucan transferase (EXT) (EXGT-A1) identical to |
| endo-xyloglucan transferase (ext) GI: 469484 and endoxyloglucan transferase (EXGT-A1) GI: 5533309 from [Arabidopsis thaliana] | |||||
| (68415.m00767) | |||||
| 45 | (D) External Glu repression | NC-NC-D | 266093_at | At2g37990 | ribosome biogenesis regulatory protein (RRS1) family protein contains Pfam profile PF04939: Ribosome biogenesis regulatory |
| protein (RRS1); similar to Ribosome biogenesis regulatory protein homolog (Swiss-Prot: Q15050) [Homo sapiens] (68415.m04663) | |||||
| 46 | (D) External Glu repression | NC-NC-D | 264738_at | At1g62250 | expressed protein (68414.m07022) |
| 47 | (D) External Glu repression | NC-NC-D | 264360_at | At1g03310 | isoamylase, putative/starch debranching enzyme, putative similar to isoamylase isoform 2 [Solanum tuberosum] GI: 27728147. |
| isoamylase from [Triticum aestivum] GI: 17932898, [Hordeum vulgare] GI: 21314275, [Oryza sativa] GI: 3252794; contains Pfam | |||||
| profiles PF00128: Alpha amylase catalytic domain, PF02922: Isoamylase N-terminal domain; ESTs gb|H36690, gb|AA712462, | |||||
| gb|AA651230 and gb|N95932 come from this gene (68414.m00309) | |||||
| 48 | (D) External Glu repression | NC-NC-D | 263957_at | At2g35880 | expressed protein (68415.m04405) |
| 49 | (D) External Glu repression | NC-NC-D | 263924_at | At2g36530 | enolase identical to SWISS-PROT: P25696 enolase (EC 4.2.1.11) (2-phosphoglycerate dehydratase)(2-phospho-D-glycerate hydrolyase) |
| [Arabidopsis thaliana] (68415.m04481) | |||||
| 50 | (D) External Glu repression | NC-NC-D | 283714_at | At2g20610 | aminotransferase, putative similar to nicotianamine aminotransferase from Hordeum vulgare [GI: 6498122, GI: 6469087]; contains |
| Pfam profile PF00155 aminotransferase, classes I and II (68415.m02411) | |||||
| 51 | (D) External Glu repression | NC-NC-D | 263477_at | At2g31790 | UDP-glucoronosyl/UDP-glucosyl transferase family protein contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl |
| transferase (68415.m03881) | |||||
| 52 | (D) External Glu repression | NC-NC-D | 262947_at | At1g75750 | gibberellin-regulated protein 1 (GASA1)/gibberellin-responsive protein 1 identical to SP|P46689 Gibberellin-regulted protein 1 |
| precursor {Arabidopsis thaliana}; supporting cDNA gi|887938|gb|U11766.1|ATU11766 (68414.m08798) | |||||
| 53 | (D) External Glu repression | NC-NC-D | 262840_at | At1g14900 | high-mobility-group protein/HMG-I/Y protein nearly identical to high-mobility-group protein HMG-I/Y protein [Arabidopsis thaliana] |
| GI: 1429211; contains Pfam profiles PF00538: linker histone H1 and H5 family, PF02178: AT hook motif (68414.m01781) | |||||
| 54 | (D) External Glu repression | NC-NC-D | 262433_s_at | At1g47500 | RNA-binding protein 47 (RBP47), putative similar to DNA binding protein GI: 1899187 from [Nicotiana tabacum] (68414.m05272) |
| 55 | (D) External Glu repression | NC-NC-D | 262274_at | At1g68720 | cytidine/deoxycytidylate deaminase family protein contains Pfam profile PF00383: Cytidine and deoxycytidylte deaminase zinc- |
| binding region (68414.m07851) | |||||
| 56 | (D) External Glu repression | NC-NC-D | 26094_at | At1g56110 | nucleolar protein Nop56, putative similar to XNop56 protein [Xenopus laevis] GI: 14799394; contains Pfam profile PF01798: |
| Putative snoRNA binding domain (68414.m06443) | |||||
| 57 | (D) External Glu repression | NC-NC-D | 261859_at | At1g50490 | ubiquitin-conjugating enzyme 20 (UBC20) nearly identical to ubiquitin-conjugating enzyme UBC20 [Arabidopsis thaliana] |
| GI: 22530867; contains Pfam profile PF00179: Ubiquitin-conjugating enzyme (68414.m05682) | |||||
| 58 | (D) External Glu repression | NC-NC-D | 261603_at | At1g9600 | RNA-binding protein 47 (RBP47), putative similar to DNA binding protein ACBF GB: U90212 GI: 1899187 from [Nicotiana tabacum] |
| (68414.m05561) | |||||
| 59 | (D) External Glu repression | NC-NC-D | 261350_at | At1g79770 | expressed protein (68414.m09308) |
| 60 | (D) External Glu repression | NC-NC-D | 260967_at | At1g12230 | transaldolase, putative similar to Swiss-Prot: P30148 transaldolase B (EC 2.2.1.2) [Escherichia coli O157: H7] (68414.m01415) |
| 61 | (D) External Glu repression | NC-NC-D | 260830_at | At1g06760 | histone H1, putative similar to histone H1-1 GB: CAA44312 GI: 16314 from [Arabidopsis thaliana]; identical to cDNA H1-1C mRNA |
| for histone H1-1 (partial) GI: 732560 (68414.m00718) | |||||
| 62 | (D) External Glu repression | NC-NC-D | 260637_at | At1g62380 | 1-aminocyclopropane-1-carboxylate oxidase, putative/ACC oxidase, putative nearly identical to ACC oxidase (ACC ox1) |
| GI: 587086 from [Brassica oleracea] (68414.m07038) | |||||
| 63 | (D) External Glu repression | NC-NC-D | 260615_at | At1g53240 | malate dehydrogenase [NAD], mitochondrial identical to mitochondrial NAD-dependent malate dehydrogenase GI: 3929649 |
| SP|Q9ZP06 from [Arabidopsis thaliana]; contains InterPro entry IPR001236: Lactate/malate dehydrogenase (68414.m06033) | |||||
| 64 | (D) External Glu repression | NC-NC-D | 260487_at | At1g51510 | RNA-binding protein, putative similar to RNA-binding protein 8 (Ribonucleoprotein RBM8) SP: Q9Y5S9 from [Homo sapiens], RNA- |
| binding protein Y14 [Xenopus laevis] GI: 11034807; contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA | |||||
| recognition motif) (RRM) (68414.m05797) | |||||
| 65 | (D) External Glu repression | NC-NC-D | 260385_at | At1g74090 | sulfotransferase family protein similar to SP|P52837 Flavonol 4′-sulfotransferase (EC 2.8.2.—) (F4-ST) {Flaveria chloraefolia}; |
| contains Pfam profile PF00685: Sulfotransferase domain (68414.m08581) | |||||
| 66 | (D) External Glu repression | NC-NC-D | 260347_at | At1g69420 | zinc finger (DHHC type) family protein contains Pfam profile: PF01529: DHHC zinc finger domain (68414.m07974) |
| 67 | (D) External Glu repression | NC-NC-D | 260285_at | At1g80560 | 3-isopropylmalate dehydrogenase, chloroplast, putative strong similarity to 3-ISOPROPYLMALATE DEHYDROGENASE |
| PRECURSOR GB: P29102 SP|P29102 from [Brassica napus] (68414.m09445) | |||||
| 68 | (D) External Glu repression | NC-NC-D | 259375_at | At3g16370 | GDSL-motif lipase/hydrolase family protein similar to family II lipases EXL3 GI: 15054386, EXL1 GI: 15054382, EXL2 GI: 15054384 |
| from [Arabidopsis thaliana]; contains Pfam profile: PF00657 Lipase Acylhydrolase with GDSL-like motif (68416.m02071) | |||||
| 69 | (D) External Glu repression | NC-NC-D | 259381_s_at | At3g16390 | jacalin lectin family protein similar to myrosinase-binding protein homolog [Arabidopsis thaliana] GI: 2997767, epithiospecifier |
| [Arabidopsis thaliana] GI: 16118845; contains Pfam profiles PF01419 jacalin-like lectin family, PF01344 Ketch motif | |||||
| (68416.m02075) | |||||
| 70 | (D) External Glu repression | NC-NC-D | 258920_at | At3g10520 | non-symbiotic hemoglobin 2 (HB2) (GLB2) identical to SP|O24521 Non-symbiotic hemoglobin 2 (Hb2) (ARAth GLB2) {Arabidopsis |
| thaliana} (68416.m01262) | |||||
| 71 | (D) External Glu repression | NC-NC-D | 258521_at | At3g06680 | 60S ribosomal protein L29 (RPL29B) similar to 60S ribosomal protein L29 GB: P25886 from (Rattus norvegicus) (68416.m00788) |
| 72 | (D) External Glu repression | NC-NC-D | 258208_at | At3g13930 | dihydrolipoamide S-acetyltransferase, putative similar to dihydrolipoamide S-acetytransferase [Zea mays] GI: 5669871; contains |
| Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin-requiring enzyme, | |||||
| PF02817: e3 binding domain (68416.m01759) | |||||
| 73 | (D) External Glu repression | NC-NC-D | 257822_at | At3g25230 | peptidyl-prolyl cis-trans isomerase/FK506-binding protein (ROF1) identical to rotamase FKBP (ROF1) GB: U49453 [Arabidopsis |
| thaliana] (Mol. Gen. Genet. 252 (5), 510-517 (1996)) (68416.m03152) | |||||
| 74 | (D) External Glu repression | NC-NC-D | 257774_at | At3g29250 | short-chain dehydrogenase/reductase (SDR) family protein similar to 3-beta-hydroxysteroiddehydrogenase GI: 15983819 from |
| [Digitalis lanata]; contains Pfam profile: PF00106 short chain dehydrogenase (68416.m03670) | |||||
| 75 | (D) External Glu repression | NC-NC-D | 257311_at | At3g26570 | phosphate transporter family protein contains Pfam profile: PF01384 phosphate transporter family (68416.m03316) |
| 76 | (D) External Glu repression | NC-NC-D | 257174_at | At3g27190 | uracil phosphoribosyltransferase, putative/UMP pyrophosphorylase, putative/UPRTase, putative similar to SP|O65583 Uracil |
| phosphoribosyltransferase (EC 2.4.2.9) (UMP pyrophosphorylase) (UPRTase) {Arabidopsis thaliana}; contains Pfam profile | |||||
| PF00485: Phosphoribulokinase/Uridine kinase family (68416.m03400) | |||||
| 77 | (D) External Glu repression | NC-NC-D | 257125_at | At3g20050 | T-complex protein 1 alpha subunit/TCP-1-alpha/chaperonin (CCT1) identical to SWISS-PROT: P28769-T-complex protein 1, |
| alpha subunit (TCP-1-alpha) [Arabidopsis thaliana] (68416.m02536) | |||||
| 78 | (D) External Glu repression | NC-NC-D | 256628_at | At3g20000 | porin family protein low similarity to haymaker protein [Mus musculus] GI: 17834089, mitochondrial outer membrane protein MOM35 |
| [Mus musculus] GI: 6650562; contains Pfam profile PF01459: Eukaryotic porin (68416.m02530) | |||||
| 79 | (D) External Glu repression | NC-NC-D | 256547_at | At3g14840 | leucine-rich repeat family protein/protein kinase family protein contains Pfam domains PF00560: Leucine Rich Repeat and |
| PF00069: Protein kinase domain; contains 2 predicted transmembrane domains (68416.m01874) | |||||
| 80 | (D) External Glu repression | NC-NC-D | 256341_at | At1g72040 | deoxynucleoside kinase family contains Pfam profile: PF01712 deoxynucleoside kinase (68414.m08327) |
| 81 | (D) External Glu repression | NC-NC-D | 256299_at | At1g89530 | expansin, putative (EXP1) identical to expansin (At-EXP1) [Arabidopsis thaliana] GI: 1041702; alpha-expansin gene family, |
| PMID: 11641069 (68414.m07993) | |||||
| 82 | (D) External Glu repression | NC-NC-D | 256072_at | At1g18080 | WD-40 repeat family protein/auxin-dependent protein (ARCA)/guanine nucleotide-binding protein beta subunit, putative identical |
| to SP|O24456 Guanine nucleotide-binding protein beta subunit-like protein (WD-40 repeat auxin-dependent protein ARCA) | |||||
| {Arabidopsis thaliana}; contains 7 WD-40 repeats (PF00400) (68414.m02238) | |||||
| 83 | (D) External Glu repression | NC-NC-D | 255783_at | At1g19870 | calmodulin-binding family protein contains Pfam profile: PF00612 IQ calmodulin-binding motif (68414.m02492) |
| 84 | (D) External Glu repression | NC-NC-D | 255482_at | At4g02510 | chloroplast outer membrane protein, putative similar to chloroplast protein import component Toc159 [Pisum sativum] GI: 8489806, |
| chloroplast outer envelope protein 86 [Pisum sativum] GI: 599958, GTP-binding protein [Pisum sativum] GI: 576509 (68417.m00343) | |||||
| 85 | (D) External Glu repression | NC-NC-D | 255052_at | At4g09720 | Ras-related GTP-binding protein, putative similar to GTP-binding protein RAB7A from [Lotus japonicus] (68417.m01596) |
| 86 | (D) External Glu repression | NC-NC-D | 255020_at | At4g10320 | isoleucyl-tRNA synthetase, putative/isoleucine-tRNA ligase, putative similar to SP|P41252 Isoleucyl-tRNA synthetase, |
| cytoplasmic (EC 6.1.1.5) (Isoleucine-tRNA ligase) (IleRS) (IRS) {Homo sapiens}; contains Pfam profile PF00133: tRNA | |||||
| synthetases class I (I, L, M and V) (68417.m01697) | |||||
| 87 | (D) External Glu repression | NC-NC-D | 254981_at | At4g10480 | nascent polypeptide associated complex alpha chain protein, putative/alpha-NAC, putative similar to alpha-NAC, non-muscle form |
| [Mus musculus] GI: 1666690; contains Pfam profiles PF01849: NAC domain, PF00627: UBA/TS-N domain (68417.m01720) | |||||
| 88 | (D) External Glu repression | NC-NC-D | 254811_at | At4g12230 | esterase/lipase/thioesterase family protein low similarity to 2-hydroxy-6-ketonona-2,4-dienedoic acid hydrolase; OhpC |
| [Rhodococcus sp.] GI: 8926386; contains Interpro entry IPR000379 (68417.m01940) | |||||
| 89 | (D) External Glu repression | NC-NC-D | 254887_at | At4g13770 | cytochrome P450 family protein (68417.m02136) |
| 90 | (D) External Glu repression | NC-NC-D | 254609_at | At4g18970 | GDSL-motif lipase/hydrolase family protein similar to family II lipases EXL3 GI: 15054386, EXL1 GI: 15054382, EXL2 GI: 15054384 |
| from [Arabidopsis thaliana]; contains Pfam profile PF00657: GDSL-like Lipase/Acylhydrolase (68417.m02794) | |||||
| 91 | (D) External Glu repression | NC-NC-D | 254372_at | At4g21620 | glycine-rich protein (68417.m03134) |
| 92 | (D) External Glu repression | NC-NC-D | 254080_at | At4g25630 | fibrillarin 2 (FIB2) identical to fibrillarin 2 GI: 9965655 from [Arabidopsis thaliana] (68417.m03691) |
| 93 | (D) External Glu repression | NC-NC-D | 253777_at | At4g28450 | transducin family protein/WD-40 repeat family protein SOF1 (involved in rRNA processing) protein-yeast (68417.m04071) |
| 94 | (D) External Glu repression | NC-NC-D | 253666_at | At4g30270 | MERI-5 protein (MERI-5) (MERI58)/endo-xyloglucan transferase/xyloglucan endo-1,4-beta-D-glucanase (SEN4) identical to endo |
| xyloglucan transferase gi: 944810, SP|P24806 MERI-5 protein precursor (Endo-xyloglucan transferase) (Xyloglucan endo-1,4-beta- | |||||
| D-glucanase) {Arabidopsis thaliana} (68417.m04303) | |||||
| 95 | (D) External Glu repression | NC-NC-D | 253562_at | At4g31130 | expressed protein (68417.m04419) |
| 96 | (D) External Glu repression | NC-NC-D | 253233_at | At4g34290 | SWIB complex BAF60b domain-containing protein contains Pfam profile PF02201: BAF60b domain of the SWIB complex |
| (68417.m04874) | |||||
| 97 | (D) External Glu repression | NC-NC-D | 253013_at | At4g37910 | heat shock protein 70, mitochondrial, putative/HSP70, mitochondrial, putative strong similarity to SP|Q01899 Heat shock 70 kDa |
| protein, mitochondrial precursor {Phaseotus vulgaris} (68417.m05361) | |||||
| 98 | (D) External Glu repression | NC-NC-D | 252712_at | At3g43800 | glutathione S-transferase, putative glutathione transferase, papaya, PIR: T09781 (68416.m04681) |
| 99 | (D) External Glu repression | NC-NC-D | 252661_at | At3g44450 | expressed protein (68416.m04777) |
| 100 | (D) External Glu repression | NC-NC-D | 252492_at | At3g46740 | chloroplast outer envelope protein, putative similar to chloroplastic outer envelope membrane protein (OEP75) [Pisum sativum] |
| GI: 633607; contains Pfam profile PF01103: outer membrane protein, OMP85 family (68416.m05074) | |||||
| 101 | (D) External Glu repression | NC-NC-D | 252327_at | At3g48740 | nodulin MtN3 family protein similar to MtN3 GI: 1619602 (root nodule development) from [Medicago truncatula] (68416.m05322) |
| 102 | (D) External Glu repression | NC-NC-D | 252239_at | At3g49990 | expressed protein (68416.m05466) |
| 103 | (D) External Glu repression | NC-NC-D | 252182_at | At3g50670 | U1 small nuclear ribonucleoprotein 70 (U1-70k) (68416.m05542) |
| 104 | (D) External Glu repression | NC-NC-D | 252115_at | At3g51600 | nonspecific lipid transfer protein 5 (LTP5) identical to SP|Q9XFS7 Nonspecific lipid-transfer protein 5 (LTP 5) {Arabidopsis thaliana} |
| (68416.m05654) | |||||
| 105 | (D) External Glu repression | NC-NC-D | 251963_at | At3g53570 | protein kinase (AFC1) (AME2) identical to protein kinase AFC1 (EC 2.7.1.—) [Arabidopsis thaliana] (68416.m05915) |
| 106 | (D) External Glu repression | NC-NC-D | 251638_at | At3g57490 | 40S ribosomal protein S2 (RPS2D) 40S ribosomal protein S2 - Arabidopsis thaliana, SWISSPROT: RS2_ARATH (68416.m06400) |
| 107 | (D) External Glu repression | NC-NC-D | 251322_at | At3g61440 | cysteine synthase, putative/O-acetylserine (thiol)-lyase, putative/O-acetylserine sulfhydrylase, putative identical to cysteine |
| synthase (EC 4.2.99.8) [Arabidopsis thaliana] GI: 5824334; contains Pfam profile PF00291: Pyridoxal-phosphate dependent | |||||
| enzyme (68416.m06881) | |||||
| 108 | (D) External Glu repression | NC-NC-D | 251264_at | At3g62120 | tRNA synthetase class II (G, H, P and S) family protein similar to SP|P07814 Bifunctional aminoacyl-tRNA synthetase [Includes: |
| Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate-tRNA ligase); Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline-tRNA ligase)] | |||||
| {Homo sapiens}; contains Pfam profiles PF00587: tRNA synthetase class II core domain (G, H, P, S and T), PF03129: Anticodon | |||||
| binding domain (68416.m06979) | |||||
| 109 | (D) External Glu repression | NC-NC-D | 250973_at | At5g02870 | 60S ribosomal protein L4/L1 (RPL4D) 60S roibosomal protein L4, Arabidopsis thaliana, EMBL: CAA79104 (68418.m00230) |
| 110 | (D) External Glu repression | NC-NC-D | 250936_at | At5g03120 | expressed protein (68418.m00260) |
| 111 | (D) External Glu repression | NC-NC-D | 250918_at | At5g03610 | GDSL-motif lipase/hydrolase family protein low similarity to SP|P40602 Anther-specific proline-rich protein APG precursor |
| {Arabidopsis thaliana}; contains Pfam profile PF00657: GDSL-like Lipase/Acylhydrolase (68418.m00320) | |||||
| 112 | (D) External Glu repression | NC-NC-D | 250832_at | At5g04950 | nicotianamine synthase, putative similar to nicotianamine synthase [Lycopersicon esculentum][GI: 4753801], nicotianamine |
| synthase 2 [Hordeum vulgare][GI: 4894912] (68418.m00524) | |||||
| 113 | (D) External Glu repression | NC-NC-D | 248797_at | At5g47210 | nuclear RNA-binding protein, putative similar to nuclear RNA binding protein GI: 6492264 from [Arabidopsis thaliana] |
| (68418.m05821) | |||||
| 114 | (D) External Glu repression | NC-NC-D | 248582_at | At5g49910 | heat shock protein 70/HSP70 (HSC70-7) identical to heat shock protein 70 [Arabidopsis thaliana] GI: 6746592 (68418.m06180) |
| 115 | (D) External Glu repression | NC-NC-D | 247965_at | At5g56540 | arabinogalactan-protein (AGP14) identical to gi|10880505|gb|AAG24262 (68418.m07056) |
| 116 | (D) External Glu repression | NC-NC-D | 247900_at | At5g57290 | 60S acidic ribosomal protein P3 (RPP3B) (68418.m07157) |
| 117 | (D) External Glu repression | NC-NC-D | 247569_at | At5g61240 | leucine-rich repeat family protein contains leucine rich-repeat (LRR) domains Pfam: PF00560, INTERPRO: IPR001611; contains |
| similarity to Hcr2-0B [Lycopersicon esculentum] gi|3894387|gb|AAC78593 (68418.m07681) | |||||
| 118 | (D) External Glu repression | NC-NC-D | 247157_at | At5g65770 | nuclear matrix constituent protein-related low similarity to nuclear matrix constituent protein 1 (NMCP1) [Daucus carota] GI: 2190187 |
| (68418.m08276) | |||||
| 119 | (D) External Glu repression | NC-NC-D | 247076_at | At5g66510 | bacterial transferase hexapeptide repeat-containing protein contains Pfam profile PF00132: Bacterial transferase hexapeptide (four |
| repeats) (68418.m08386) | |||||
| 120 | (D) External Glu repression | NC-NC-D | 246860_at | At5g25840 | expressed protein (68418.m03066) |
| 121 | (D) External Glu repression | NC-NC-D | 246845_at | At5g26707 | |
| 122 | (D) External Glu repression | NC-NC-D | 246809_s_at | At5g27140 | SAR DNA-binding protein, putative strong similarity to SAR DNA-binding protein-1 [Pisum sativum] GI: 3132696; contains Pfam |
| profile PF01798: Putative snoRNA binding domain (68418.m03239) | |||||
| 123 | (D) External Glu repression | NC-NC-D | 245714_at | At5g04280 | glycine-rich RNA-binding protein (68418.m00421) |
| 124 | (D) External Glu repression | NC-NC-D | 245350_at | At4g16830 | nuclear RNA-binding protein (RGGA) identical to nuclear RNA binding protein GI: 6492264 from [Arabidopsis thaliana] |
| (68417.m02540) | |||||
| 125 | (D) External Glu repression | NC-NC-D | 245277_at | At4g15550 | UDP-glucose:indole-3-acetate beta-D-glucosyltransferase (IAGLU) identical to UDP-glucose:indole-3-acetate beta-D- |
| glucosyltransferase (iaglu) GI: 2149126 from [Arabidopsis thaliana] (68417.m02376) | |||||
| 126 | (D) External Glu repression | NC-NC-D | 244980_at | rpl36 | ribosomal protein L36 |
| 1 | (E) Internal/External Glu | I-D-I | 249923_at | At5g19120 | expressed protein low similarity to extracellular dermal glycoprotein EDGP precursor [Daucus carota] GI: 285741, SP|P13917 Basic |
| induction | 7S globulin precursor {Glycine max} (68418.m02275) | ||||
| 2 | (E) Internal/External Glu | I-D-I | 249862_at | At5g22920 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profiles: PF05495 CHY zinc finger, PF00097 zinc finger, C3HC4 |
| induction | type (RING finger) (68418.m02680) | ||||
| 3 | (E) Internal/External Glu | I-D-I | 246114_at | At5g20250 | raffinose synthase family protein/seed imbibition protein, putative (din10) similar to seed imbibition protein GB: AAA32975 |
| induction | GI: 167100 from [Hordeum vulgare]; contains nonconsensus AT donor splice site at intron 1; contains Pfam profile PF05691: | ||||
| Raffinose synthase or seed imbibition protein Sip1; identical to cDNA seed imbibition protein (din10) partial cds GI: 10834551 | |||||
| (68418.m02410) | |||||
| 4 | (E) Internal/External Glu | I-NC-I | 267461_at | At2g33830 | dormancy/auxin associated family protein contains Pfam profile: PF05564 dormancy/auxin associated protein (68415.m04150) |
| induction | |||||
| 5 | (E) Internal/External Glu | I-NC-I | 266995_at | At2g34500 | cytochrome P450 family protein similar to Cytochrome P450 61 (C-22 sterol desaturase) (SP: P54781) {Saccharomyces cerevisiae} |
| induction | (68415.m04237) | ||||
| 6 | (E) Internal/External Glu | I-NC-I | 266984_at | At2g39570 | ACT domain-containing protein contains Pfam ACT domain PF01842 (68415.m04854) |
| induction | |||||
| 7 | (E) Internal/External Glu | I-NC-I | 266259_at | At2g27830 | expressed protein (68415.m03374) |
| induction | |||||
| 8 | (E) Internal/External Glu | I-NC-I | 265111_at | At1g62510 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein contains Pfam protease inhibitor/seed storage/LTP family |
| induction | domain PF00234 (68414.m07053) | ||||
| 9 | (E) Internal/External Glu | I-NC-I | 264524_at | At1g10070 | branched-chain amino acid aminotransferase 2/branched-chain amino acid transaminase 2 (BCAT2) identical to SP|Q9M439 |
| induction | Branched-chain amino acid aminotransferase 2, chloroplast precursor (EC 2.6.1.42) (Atbcat-2) {Arabidopsis thaliana}; contains | ||||
| Pfam profile: PF01063 aminotransferase class IV (68414.m01136) | |||||
| 10 | (E) Internal/External Glu | I-NC-I | 263157_at | At1g54100 | aldehyde dehydrogenase, putative/antiquitin, putative strong similarity to SP|Q41247 Aldehyde dehydrogenase family 7 member |
| induction | A1 (EC 1.2.1.3) (Antiquitin 1) (Brassica turgor-responsive/drought-induced gene 26 protein) (Btg-26) {Brassica napus}; similar to | ||||
| turgor-responsive protein 28G (aldehyde dehydrogenase family 7 member A1) [Pisum sativum] SWISS-PROT: P25795 | |||||
| (68414.m06166) | |||||
| 11 | (E) Internal/External Glu | I-NC-I | 262399_at | At1g49500 | expressed protein (68414.m05548) |
| induction | |||||
| 12 | (E) Internal/External Glu | I-NC-I | 261135_at | At1g19610 | plant defensin-fusion protein, putative (PDF1.4) plant defensin protein family member, personal communication, Bart Thomma |
| induction | (Bart.Thomma@agr.kuleuven.ac.be); similar to SWISS-PROT: P30224, Cysteine-rich antifungal protein 1 precursor | ||||
| (AFP1)[Arabidopsis thaliana] (68414.m02443) | |||||
| 13 | (E) Internal/External Glu | I-NC-I | 259431_at | At1g01620 | plasma membrane intrinsic protein 1C (PIP1C)/aquaporin PIP1.3 (PIP1.3)/transmembrane protein B (TMPB) identical to plasma |
| induction | membrane intrinsic protein 1c SP: Q08733 from [Arabidopsis thaliana] (68414.m00079) | ||||
| 14 | (E) Internal/External Glu | I-NC-I | 258939_at | At3g10020 | expressed protein (68416.m01202) |
| induction | |||||
| 15 | (E) Internal/External Glu | I-NC-I | 258527_at | At3g06850 | branched chain alpha-keto acid dehydrogenase E2 subunit (din3) identical to branched chain alpha-keto acid dehydrogenase E2 |
| induction | subunit (din3) [Arabidopsis thaliana] GI: 7021284 (68416.m00812) | ||||
| 16 | (E) Internal/External Glu | I-NC-I | 258402_at | At3g15450 | expressed protein similar to auxin down-regulated protein ARG10 [Vigna radiata] GI: 2970051, wali7 (aluminum-induced protein) |
| induction | [Triticum aestivum] GI: 451193 (68416.m01960) | ||||
| 17 | (E) Internal/External Glu | I-NC-I | 258225_at | At3g15630 | expressed protein (68416.m01982) |
| induction | |||||
| 16 | (E) Internal/External Glu | I-NC-I | 258005_at | At3g19390 | cysteine proteinase, putative/thiol protease, putative contains similarity to cysteine proteinase RD21A (thiol protease) GI: 435619, |
| induction | SP: P43297 from [Arabidopsis thaliana] (68416.m02459) | ||||
| 19 | (E) Internal/External Glu | I-NC-I | 256894_at | At3g21870 | cyclin family protein similar to cyclin 2 [Trypanosoma brucei] GI: 7339572, cyclin 6 [Trypanosoma cruzi] GI: 12005317; contains |
| induction | Pfam profile PF00134: Cyclin, N-terminal domain (68416.m02758) | ||||
| 20 | (E) Internal/External Glu | I-NC-I | 253279_at | At4g34030 | methylcrotonyl-CoA carboxylase beta chain, mitochondrial/3-methylcrotonyl-CoA carboxylase 2 (MCCB) identical to SP|Q9LDD8 |
| induction | Methylcrotonyl-CoA carboxylase beta chain, mitochondrial precursor (EC 6.4.1.4) (3-Methylcrotonyl-CoA carboxylase 2) (MCCase | ||||
| beta subunit) (3-methylcrotonyl-CoA:carbon dioxide ligase beta subunit) {Arabidopsis thaliana}; contains Pfam profile: PF01039 | |||||
| carboxyl transferase domain (68417.m04829) | |||||
| 21 | (E) Internal/External Glu | I-NC-I | 253061_at | At4g37610 | TAZ zinc finger family protein/BTB/POZ domain-containing protein contains Pfam PF00851: BTB/POZ domain; contains Pfam |
| induction | PF02135: TAZ zinc finger; similar to Speckle-type POZ protein (SP: O43791) [Homo sapiens] (68417.m05321) | ||||
| 22 | (E) Internal/External Glu | I-NC-I | 252415_at | At3g47340 | asparagine synthetase 1 [glutamine-hydrolyzing]/glutamine-dependent asparagine synthetase 1 (ASN1) identical to SP|P49078 |
| induction | Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4) (Glutamine-dependent asparagine synthetase) {Arabidopsis thaliana} | ||||
| (68416.m05145) | |||||
| 23 | (E) Internal/External Glu | I-NC-I | 251642_at | At3g57520 | alkaline alpha galactosidase, putative similar to alkaline alpha galactosidase II [Cucumis melo] GI: 29838631; contains Pfam profile |
| induction | PF05691: Raffinose synthase or seed imbibition protein Sip1 (68416.m06403) | ||||
| 24 | (E) Internal/External Glu | I-NC-I | 248606-at | At5g49450 | bZIP family transcription factor similar to bZIP transcription factor GI: 1769891 from [Arabidopsis thaliana] (68418.m06118) |
| induction | |||||
| 25 | (E) Internal/External Glu | I-NC-I | 247585_at | At5g60680 | expressed protein contains Pfam profile PF04520: Protein of unknown function, DUF584 (68418.m07615) |
| induction | |||||
| 26 | (E) Internal/External Glu | NC-D-I | 255742_at | At1g25560 | AP2 domain-containing transcription factor, putative similar to DNA-binding protein RAV2 GI: 3868859 from [Arabidopsis thaliana] |
| induction | (68414.m03173) | ||||
| 27 | (E) Internal/External Glu | NC-D-NC | 267523_at | At2g30610 | |
| induction | |||||
| 28 | (E) Internal/External Glu | NC-D-NC | 267337_at | At2g39980 | transferase family protein contains Pfam profile PF02458 transferase family (68415.m04913) |
| induction | |||||
| 29 | (E) Internal/External Glu | NC-D-NC | 267230_at | At2g44080 | expressed protein (68415.m05482) |
| induction | expansin, putative (EXP3) identical to Alpha-expansin 3 precursor (AI-EXP3)[Arabidopsis thaliana] SWISS-PROT: O80932; alpha- | ||||
| 30 | (E) Internal/External Glu | NC-D-NC | 267158_at | At2g37640 | expansin gene family, PMID: 11641069 (68415.m04617) |
| induction | |||||
| 31 | (E) Internal/External Glu | NC-D-NC | 266463_at | At2g47840 | tic20 protein-related similar to Tic20 (GI: 3769673) [Pisum sativum] (68415.m05971) |
| induction | |||||
| 32 | (E) Internal/External Glu | NC-D-NC | 265384_at | At2g20760 | expressed protein (68415.m02440) |
| induction | |||||
| 33 | (E) Internal/External Glu | NC-D-NC | 264508_at | At1g09570 | phytochrome A (PHYA) identical to SP|P14712 Phytochrome A {Arabidopsis thaliana} (68414.m01073) |
| induction | |||||
| 34 | (E) Internal/External Glu | NC-D-NC | 264188_at | At1g54690 | histone H2A, putative strong similarity to histone H2A GI: 3204129 SP|O65759 from Cicer arietinum, Picea abies SP|P35063; |
| induction | contains Pfam profile PF00125 Core histone H2A/H2B/H3/H4 (68414.m06235) | ||||
| 35 | (E) Internal/External Glu | NC-D-NC | 263710_at | At1g09330 | expressed protein contains 3 transmembrane domains; contains Pfam profile PF05832: Eukaryotic protein of unknown function |
| induction | (68414.m01044) | ||||
| 36 | (E) Internal/External Glu | NC-D-NC | 263597_at | At2g01870 | expressed protein (68415.m00120) |
| induction | |||||
| 37 | (E) Internal/External Glu | NC-D-NC | 262215_at | At1g74790 | expressed protein contains similarity to hedgehog-interacting protein GI: 4868122 from [Mus musculus] (68414.m08685) |
| induction | |||||
| 38 | (E) Internal/External Glu | NC-D-NC | 262024_at | At1g35620 | thioredoxin family protein similar to SP|Q43116 Protein disulfide isomerase precursor (PDI) (EC 5.3.4.1) {Ricinus communis}; |
| induction | contains Pfam profile PF00085: Thioredoxin (68414.m04425) | ||||
| 39 | (E) Internal/External Glu | NC-D-NC | 261937_at | At1g22570 | proton-dependent oligopeptide transport (POT) family protein contains Pfam profile: PF00854 POT family (68414.m02818) |
| induction | |||||
| 40 | (E) Internal/External Glu | NC-D-NC | 260410_at | At1g69870 | proton-dependent oligopeptide transport (POT) family protein contains Pfam profile: PF00854 POT family (68414.m08041) |
| induction | |||||
| 41 | (E) Internal/External Glu | NC-D-NC | 260266_at | At1g68520 | zinc finger (B-box type) family protein contains Pfam profile: PF00643 B-box zinc finger (68414.rn07827) |
| induction | |||||
| 42 | (E) Internal/External Glu | NC-D-NC | 260037_at | At1g68840 | DNA-binding protein RAV2 (RAV2)/AP2 domain-containing protein RAP2.8 identical to RAV2 GI: 3868859 from [Arabidopsis |
| induction | thaliana], AP2 domain containing protein RAP2.8 [Arabidopsis thaliana] GI: 2281641; contains Pfam profile: PF00847 AP2-domain | ||||
| (68414.m07873) | |||||
| 43 | (E) Internal/External Glu | NC-D-NC | 259504_at | At1g15690 | pyrophosphate-energized vacuolar membrane proton pump/pyrophosphate-energized inorganic pyrophosphatase (AVP-3) |
| induction | identical to pyrophosphate-energized vacuolar membrane proton pump (pyrophosphate-energized inorganic pyrophosphatase) | ||||
| SP: P31414 from [Arabidopsis thaliana] (68414.m01883) | |||||
| 44 | (E) Internal/External Glu | NC-D-NC | 258809_at | At3g04070 | no apical meristem (NAM) family protein contains Pfam PF02365: No apical meristem (NAM) domain; similar to NAM |
| induction | GB: CAA63101 [Petunia x hybrida] (68416.m00430) | ||||
| 45 | (E) Internal/External Glu | NC-D-NC | 258299_at | At3g23410 | alcohol oxidase-related similar to long chain fatty alcohol oxidase from Candida cloacae [GI: 698351], Candida tropicalis |
| induction | [GI: 6983594] (68416.m02951) | ||||
| 46 | (E) Internal/External Glu | NC-D-NC | 257044_at | At3g19720 | dynamin family protein identical to cDNA dynamin-like protein (ARC5) GI: 30349145; contains Pfam profile PF00350: Dynamin |
| induction | family (68416.m02497) | ||||
| 47 | (E) Internal/External Glu | NC-D-NC | 255540_at | At4g01800 | preprotein translocase secA subunit, putative similar to preprotein translocase secA subunit, chloroplast [precursor] SP: Q9STI0 |
| induction | from [Arabidopsis thaliana]; non-consensus GA donor splice site at exon 4 (68417.m00237) | ||||
| 48 | (E) Internal/External Glu | NC-D-NC | 254456_at | At4g21150 | ribophorin II (RPN2) family protein contains Pfam domain PF05817: Ribophorin II (RPN2) (68417.m03057) |
| induction | |||||
| 49 | (E) Internal/External Glu | NC-D-NC | 254250_at | At4g23290 | protein kinase family protein contains Pfam domain PF00069: Protein kinase domain (68417.m03356) |
| induction | |||||
| 50 | (E) Internal/External Glu | NC-D-NC | 254089_at | At4g24800 | MA3 domain-containing protein similar to programmed cell death 4 protein [Gallus gallus] GI: 12958564; contains Pfam profile |
| induction | PF02847: MA3 domain (68417.m03552) | ||||
| 51 | (E) Internal/External Glu | NC-D-NC | 253871_at | At4g27440 | protochlorophyllide reductase B, chloroplast/PCR B/NADPH-protochlorophyllide oxidoreductase B (PORB) identical to |
| induction | SP: P21218 protochlorophyllide reductase B, chloroplast precursor (EC 1.3.1.33) (PCR B) (NADPH-protochlorophyllide | ||||
| oxidoreductase B) (POR B) [Arabidopsis thaliana] (68417.m03944) | |||||
| 52 | (E) Internal/External Glu | NC-D-NC | 253135_at | At4g35830 | aconitate hydratase, cytoplasmic/citrate hydro-lyase/aconitase (ACO) identical to SP|Q42560 Aconitate hydratase, cytoplasmic |
| induction | (EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase) {Arabidopsis thaliana}; contains Pfam profiles PF00330: Aconitase family (aconitate | ||||
| hydratase), PF00694: Aconitase C-terminal domain (68417.m05090) | |||||
| 53 | (E) Internal/External Glu | NC-D-NC | 252425_at | At3g47620 | TCP family transcription factor, putative auxin-induced basic helix-loop-helix transcription factor - Gossypium hirsutum, |
| induction | EMBL: AF165924 (68416.m05184) | ||||
| 54 | (E) Internal/External Glu | NC-D-NC | 252171_at | At3g50600 | |
| induction | |||||
| 55 | (E) Internal/External Glu | NC-D-NC | 251373_at | At3g60530 | zinc finger (GATA type) family protein identical to cDNA for GATA transcription factor 4 GI: 2959735 (68416.m06770) |
| induction | |||||
| 56 | (E) Internal/External Glu | NC-D-NC | 249266_at | At5g41670 | 6-phosphogluconate dehydrogenase family protein contains Pfam profiles: PF00393 6-phosphogluconate dehydrogenase C- |
| induction | terminal domain, PF03446 NAD binding domain of 6-phosphogluconate (68418.m05062) | ||||
| 57 | (E) Internal/External Glu | NC-D-NC | 248932_at | At5g46050 | proton-dependent oligopeptide transport (POT) family protein contains Pfam profile: PF00854 POT family (68418.m05663) |
| induction | |||||
| 58 | (E) Internal/External Glu | NC-D-NC | 247874_01 | At5g57710 | heat shock protein-related contains similarity to 101 kDa heat shock protein; HSP101 [Triticum aestivum] |
| induction | gi|11561808|gb|AAC83689 (68418.m07214) | ||||
| 59 | (E) Internal/External Glu | NC-D-NC | 247791_at | At5g58710 | peptidyl-protyl cis-trans isomerase, putative/cyclophilin, putative/rotamase, putative (ROC7) similar to cyclophilin [Arabidopsis |
| induction | thaliana] gi|2443755|gb|AA871401 (68418.m07355) | ||||
| 60 | (E) Internal/External Glu | NC-D-NC | 247611_at | At5g60710 | zinc finger (C3HC4-type RING finger) family protein low similarity to SP|P97280 Inter-alpha-trypsin inhibitor heavy chain H3 |
| induction | precursor {Mesocricetus auratus}; contains Pfam profiles PF00097: Zinc finger, C3HC4 type (RING finger), PF00092: von | ||||
| Willebrand factor type A domain (68418.m07618) | |||||
| 61 | (E) Internal/External Glu | NC-D-NC | 246580_at | At1g31770 | ABC transporter family protein contains Pfam profile: PF00005: ABC transporter (68414.m03899) |
| induction | |||||
| 62 | (E) Internal/External Glu | NC-D-NC | 246238_at | At4g36670 | mannitol transporter, putative similar to mannitol transporter [Apium graveolens var. dulce] GI: 12004316; contains Nam profile |
| induction | PF00083: major facilitator superfamily protein (68417.m05203) | ||||
| 63 | (E) Internal/External Glu | D-NC-D | 266892_at | At2g26080 | glycine dehydrogenase [decarboxylating], putative/glycine decarboxylase, putative/glycine cleavage system P-protein, putative |
| repression | strong similarity to SP|P26969 Glycine dehydrogenase (decarboxylating), mitochondrial precursor (EC 1.4.4.2) {Pisum sativum}; | ||||
| contains Pfam profile PF02347: Glycine cleavage system P-protein (68415.m03131) | |||||
| 64 | (E) Internal/External Glu | D-NC-D | 266805_at | At2g30010 | expressed protein (68415.m03651) |
| repression | |||||
| 65 | (E) Internal/External Glu | D-NC-D | 264956_at | At1g76990 | ACT domain containing protein low similarity to uridylyltransferase SP|P56884 from Rhizobium meliloti; contains Pfam ACT domain |
| repression | PF01842 (68414.m08964) | ||||
| 66 | (E) Internal/External Glu | D-NC-D | 264901_at | At1g23090 | sulfate transporter, putative similar to sulfate transporter [Arabidopsis thaliana] GI: 2285885; contains Pfam profiles PF00916: |
| repression | Sulfate transporter family, PF01740: STAS domain (68414.m02887) | ||||
| 67 | (E) Internal/External Glu | D-NC-D | 264903_at | At1g23190 | phosphoglucomutase, cytoplasmic, putative/glucose phosphomutase, putative strong similarity to SP|P93805 |
| repression | Phosphoglucomutase, cytoplasmic 2 (EC 5.4.2.2) (Glucose phosphomutase 2) (PGM 2) (Zea mays); contains InterPro accession | ||||
| IPR006352: Phosphoglucosamine mutase (68414.m02897) | |||||
| 68 | (E) Internal/External Glu | D-NC-D | 263865_at | At2g36910 | multidrug resistance P-glycoprotein (PGP1) identical to P-glycoprotein GI: 3849833 from [Arabidopsis thaliana]; homologous to |
| repression | mammalian mdr gene, contains ATP-binding cassette; related to multi drug resistance proteins (68415.m04527) | ||||
| 69 | (E) Internal/External Glu | D-NC-D | 263777_at | At2g46450 | cyclic nucleotide-regulated ion channel, putative (CNGC12) similar to cyclic nucleotide and calmodulin-regulated ion channel |
| repression | (cngc3) GI: 4581201 from [Arabidopsis thaliana] (68415.m05780) | ||||
| 70 | (E) Internal/External Glu | D-NC-D | 263348_at | At2g05710 | aconitate hydratase, cytoplasmic, putative/citrate hydro-lyase/aconitase, putative nearly identical to SP|P49608 Aconitate |
| repression | hydratase, cytoplasmic (EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase) {Cucurbita maxima}; contains Plant profiles PF00330: | ||||
| Aconitase family (aconitate hydratase), PF00694: Aconitase C-terminal domain (68415.m00611) | |||||
| 71 | (E) Internal/External Glu | D-NC-D | 262793_at | At1g13110 | cytochrome P450 71B7 (CYP71B7) identical to (SP: Q96514) cytochrome P450 71B7 [Arabidopsis thaliana]; PF|00067 Cytochrome |
| repression | P450 family. ESTs gb|T44875, gb|T04814, gb|R65111, gb|T44310 and gb|T04541 come from this gene; identical to cDNA | ||||
| cytochrome P450 GI: 1523795, ATCYP71B7 (68414.m01520) | |||||
| 72 | (E) Internal/External Glu | D-NC-D | 262374_s_at | At1g72930 | Toll-Interleukin-Resistance (TIR) domain-containing protein domain signature TIR exists, suggestive of a disease resistance |
| repression | protein. (68414.m08435) | ||||
| 73 | (E) Internal/External Glu | D-NC-D | 261569_at | At1g01060 | myb family transcription factor contains Pfam profile: PF00249 myb-like DNA-binding domain; identical to cDNA LATE |
| repression | ELONGATED HYPOCOTYL MYB transcription factor GI: 3281645 (68414.m00006) | ||||
| 74 | (E) Internal/External Glu | D-NC-D | 260955_at | At1g06000 | UDP-glucoronosyl/UDP-glucosyl transferase family protein contains similarity to UDPG glucosyltransferase GB: AAB62270 |
| repression | GI: 2232354 from [Solanum berthaultii] (68414.m00628) | ||||
| 75 | (E) Internal/External Glu | D-NC-D | 260913_at | At1g02500 | S-adenosylmethionine synthetase 1 (SAM1) identical to S-adenosylmethionine synthetase 1 (Methionine adenosyltransferase 1, |
| repression | AdoMet synthetase 1) [Arabidopsis thaliana] SWISS-PROT: P23686 (68414.m00200) | ||||
| 76 | (E) Internal/External Glu | D-NC-D | 260896_at | At1g29310 | protein transport protein sec61, putative similar to PfSec61 [Plasmodium falciparum] GI: 3057044; contains Pfam profile PF00344: |
| repression | eubacterial secY protein (68414.m03583) | ||||
| 77 | (E) Internal/External Glu | D-NC-D | 260162_at | At1g79830 | expressed protein weak similarity to TATA element modulatory factor (TMF) (Swiss-Prot: P82094) [Homo sapiens] (68414.m09326) |
| repression | |||||
| 78 | (E) Internal/External Glu | D-NC-D | 259958_at | At1g53730 | leucine-rich repeat transmembrane protein kinase, putative similar to GI: 3360289 from [Zea mays] (Plant Mol. Biol. 37 (5), 749-761 |
| repression | (1998)) (68414.m06114) | ||||
| 79 | (E) Internal/External Glu | D-NC-D | 259788_at | At1g29670 | GDSL-motif lipase/hydrolase family protein similar to family II lipase EXL1 GI: 15054382 from [Arabidopsis thaliana]; contains Pfam |
| repression | profile: PF00657 Lipase/Acylhydrolase with GDSL-like motif (68414.m03626) | ||||
| 80 | (E) Internal/External Glu | D-NC-D | 259228_at | At3g07720 | kelch repeat-containing protein similar to epithiospecifier (GI: 16118838) [Arabidopsis thaliana]; contains Pfam PF01344: Kelch |
| repression | motif (5 repeats) (88416.m00931) | ||||
| 81 | (E) Internal/External Glu | D-NC-D | 258609_at | At3g02910 | expressed protein contains Pfam domain PF03674: Uncharacterised protein family (UPF0131) (68416.m00286) |
| repression | |||||
| 82 | (E) Internal/External Glu | D-NC-D | 258338_at | At3g16150 | L-asparaginase, putative/L-asparagine amidohydrolase, putative similar to Swiss-Prot: P30384 L-asparaginase (EC 3.5.1.1) (L- |
| repression | asparagine amidohydrolase) [Lupinus angustifolius] (88416.m02039) | ||||
| 83 | (E) Internal/External Glu | D-NC-D | 257217_at | At3g14940 | phosphoenolpyruvate carboxylase, putative/PEP carboxylase, putative strong similarity to SP|P29198 Phosphoenolpyruvate |
| repression | carboxylase (EC 4.1.1.31) (PEPCASE) (Solanum tuberosum); contains Pfam profile PF00311: phosphoenolpyruvate carboxylase | ||||
| (68416.m01890) | |||||
| 84 | (E) Internal/External Glu | D-NC-D | 257008_at | At3g14210 | myrosinase-associated protein, putative similar to GB: CAA71238 from [Brassica napus]; contains Pfam profile: PF00857 |
| repression | Lipase/Acylhydrolase with GDSL-like motif (68416.m01796) | ||||
| 85 | (E) Internal/External Glu | D-NC-D | 256228_at | At1g56190 | phosphoglycerate kinase, putative similar to SP|P41758 Phosphoglycerate kinase, chloroplast precursor (EC 2.7.2.3) |
| repression | |||||
| 86 | (E) Internal/External Glu | D-NC-D | 255957_at | At1g22160 | {Chlamydomonas reinhardtii}; contains Pfam profile PF00162: phosphoglycerate kinase (68414.m06458) |
| repression | senescence-associated protein-related similar to senescence-associated protein SAG102 (GI: 22331931) [Arabidopsis thaliana] | ||||
| (68414.m02770) | |||||
| 87 | (E) Internal/External Glu | D-NC-D | 255615_at | At4g01290 | expressed protein (68417.m00170) |
| repression | |||||
| 88 | (E) Internal/External Glu | D-NC-D | 255421_at | At4g03260 | leucine-rich repeat family protein contains leucine rich repeat (LRR) domains. Pfam: PF00560 (68417.m00445) |
| repression | |||||
| 89 | (E) Internal/External Glu | D-NC-D | 254723_at | At4g13510 | ammonium transporter 1, member 1 (AMT1.1) identical to SP|P54144 High affinity ammonium transporter (AtAMT1; 1) {Arabidopsis |
| repression | thaliana} (68417.m02107) | ||||
| 90 | (E) Internal/External Glu | D-NC-D | 254705_at | At4g17870 | expressed protein (68417.m02664) |
| repression | |||||
| 91 | (E) Internal/External Glu | D-NC-D | 254275_at | At4g22670 | tetratricopeptide repeat (TPR)-containing protein similar to Hsc70-interacting protein (Hip) from (Homo sapiens) SP|P50502, {Rattus |
| repression | norvegicus) SP|P50503; contains Pfam profile PF00515: tetratricopeptide repeat (TPR) domain (68417.m03272) | ||||
| 92 | (E) Internal/External Glu | D-NC-D | 254174_at | At4g24120 | transporter, putative similar to iron-phytosiderophore transporter protein yellow stripe 1 [Zea mays] GI: 10770865; contains Pfam |
| repression | profile PF03169: OPT oligopeptide transporter protein (68417.m03462) | ||||
| 93 | (E) Internal/External Glu | D-NC-D | 253521_at | At4g31300 | 20S proteasome beta subunit A (PBA1)(PRCD) identical to cDNA proteasome subunit prod GI: 2511593 (68417.m04441) |
| repression | |||||
| 94 | (E) Internal/External Glu | D-NC-D | 253387_at | At4g33010 | glycine dehydrogenase [decarboxylating], putative/glycine decarboxylase, putative/glycine cleavage system P-protein, putative |
| repression | strong similarity to SP|P49361 Glycine dehydrogenase [decarboxylating] A, mitochondrial precursor (EC 1.4.4.2) {Flaveria pringlei}; | ||||
| contains Pfam profile PF02347: Glycine cleavage system P-protein (68417.m04695) | |||||
| 95 | (E) Internal/External Glu | D-NC-D | 253205_at | At4g34490 | cyclase-associated protein (cap1) identical to cyclase-associated protein (cap1) GI: 3169136 from [Arabidopsis thaliana] |
| repression | (68417.m04903) | ||||
| 96 | (E) Internal/External Glu | D-NC-D | 252827_at | At4g39950 | cytochrome P450 79B2, putative (CYP79B2) identical to cytochrome P450 (79B2) SP: O81346 from [Arabidopsis thaliana] |
| repression | (68417.m05657) | ||||
| 97 | (E) Internal/External Glu | D-NC-D | 251562_at | At3g57890 | tubulin-specific chaperone C-related contains weak similarity to Tubulin-specific chaperone C (Tubulin-folding cofactor C) (CFC) |
| repression | (Swiss-Prot: Q15814) [Homo sapiens] (68416.m06453) | ||||
| 98 | (E) Internal/External Glu | D-NC-D | 251581_at | At3g58560 | endonuclease/exonuclease/phosphatase family protein similar to SP|P31384 Glucose-repressible alcohol dehydrogenase |
| repression | transcriptional effector (Carbon catabolite repressor protein 4) {Saccharomyces cerevisiae}; contains Pfam profile PF03372: | ||||
| Endonuclease/Exonuclease/phosphatase family (68416.m06527) | |||||
| 99 | (E) Internal/External Glu | D-NC-D | 251524_at | At3g58990 | aconitase C-terminal domain-containing protein contains Pfam profile PF00694: Aconitase C-terminal domain (68416.m06575) |
| repression | |||||
| 100 | (E) Internal/External Glu | D-NC-D | 251197_at | At3g62960 | glutaredoxin family protein contains INTERPRO Domain IPR002109, Glutaredoxin (thioltransferase) (68416.m07073) |
| repression | |||||
| 101 | (E) Internal/External Glu | D-NC-D | 250337_at | At5g11790 | Ndr family protein similar to SP|O23969 Pollen specific protein SF21 (Halianthus annuus); contains Pfam profile PF03096: Ndr |
| repression | family (68418.m01376) | ||||
| 102 | (E) Internal/External Glu | D-NC-D | 250339_at | At5g11670 | matate oxidoreductase, putative similar to NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME) (SP|P12628) {Phaseolus |
| repression | vulgaris) (68418.m01364) | ||||
| 103 | (E) Internal/External Glu | D-NC-D | 250253_at | At5g13840 | lecithin:cholesterol acyltransferase family protein/LACT family protein similar to SP|P40345 Phospholipid:diacylglycerol |
| repression | acyltransferase (EC 2.3.1.158) (PDAT) (Saccharomyces cerevisiae); contains Pfam profile PF02450: Lecithin:cholesterol | ||||
| acyltransferase (phosphatidylcholine-sterol acyltransferase) (68418.m01582) | |||||
| 104 | (E) Internal/External Glu | D-NC-D | 249035_at | At5g44190 | myb family transcription factor (GLK2) contains Pfam profile: PF00249 myb-like DNA-binding domain (68418.m05407) |
| repression | |||||
| 105 | (E) Internal/External Glu | D-NC-D | 248727_at | At5g47990 | cytochrome P450 family protein similar to Cytochrome P450 93A3 (P450 CP5) (SP: O81973) [Glycine max]; (68418.m05929) |
| repression | |||||
| 106 | (E) Internal/External Glu | D-NC-D | 248729_at | At5g48010 | pentacyclic triterpene synthase, putative similar to pentacyclic triterpene synthase [gi: 6650207] [PMID: 11247608] Contains Pfam |
| repression | domain PF00432: Prenyltransferase and squalene oxidase repeat (68418.m05933) | ||||
| 107 | (E) Internal/External Glu | D-NC-D | 245736_at | At1g73330 | protease inhibitor, putative (DR4) identical to Dr4 GI: 489114 from [Arabidopsis thaliana]; contains Pfam profile PF00197: Trypsin |
| repression | and protease inhibitor (68414.m08488) | ||||
| 108 | (E) Internal/External Glu | D-NC-D | 245555_at | At4g15390 | transferase family protein similar to alcohol acyltransferase [Fragaria x ananassa][GI: 10121328][PMID: 10810141]. |
| repression | deacetylvindoline 4-O-acetyltransferase [Catharanthus roseus][GI: 4091808][PMID: 9681034] (68417.m02351) | ||||
| 109 | (E) Internal/External Glu | D-NC-D | 245021_at | psbJ | PSII component |
| repression | |||||
| 110 | (E) Internal/External Glu | NC-I-NC | 267381_at | At2g26190 | calmodulin-binding family protein contains Pfam profile PF00612: IQ calmodulin-binding motif (68415.m03145) |
| Repression | |||||
| 111 | (E) Internal/External Glu | NC-I-NC | 267005_at | At2g34460 | flavin reductase-related low similarity to SP|P30043 Flavin reductase {Homo sapiens} (68415.m04229) |
| Repression | |||||
| 112 | (E) Internal/External Glu | NC-I-NC | 265472_at | At2g15580 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) |
| Repression | (68415.m01784) | ||||
| 113 | (E) Internal/External Glu | NC-I-NC | 265194_at | At1g05010 | 1-aminocyclopropane-1-carboxylate oxidase/ACC oxidase/ethylene-forming enzyme (ACO) (EAT1) Identical to 1- |
| Repression | aminocyclopropane-1-carboxylate oxidase (ACC oxidase) gb|X66719 (EAT1). ESTs gb|T43073, gb|T5714, gb|R90435, gb|R44023, | ||||
| gb|AA597926, gb|AI099676, gb|AA650810 and gb|29725 come from this gene (68414.m00502) | |||||
| 114 | (E) Internal/External Glu | NC-I-NC | 264435_at | At1g10360 | glutathione S-transferase, putative similar to glutathione S-transferase (sp|Q03666|GTX4_TOBAC); similar to EST gb|H36275 |
| Repression | gb: AB039930. (68414.m01167) | ||||
| 115 | (E) Internal/External Glu | NC-I-NC | 264107_s_at | At2g13790 | leucine-rich repeat family protein/protein kinase family protein (68415.m01522) |
| Repression | |||||
| 116 | (E) Internal/External Glu | NC-I-NC | 263582_at | At2g17120 | peptidoglycan-binding LysM domain-containing protein contains Pfam profile P101478: LysM domain; supporting cDNA |
| Repression | gi|16226688|gb|AF428464.1|AF428484 (68415.m01976) | ||||
| 117 | (E) Internal/External Glu | NC-I-NC | 263534_at | At2g24940 | cytochrome b5 domain-containing protein similar to SP|P70580 Membrane associated progesterone receptor component 1 (Rattus |
| Repression | norvegicus) : contains Pfam profile PF00173: Heme/Steroid binding domain (68415.m02982) | ||||
| 118 | (E) Internal/External Glu | NC-I-NC | 263150_at | At1g54050 | 17.4 kDa class III heat shock protein (HSP17.4-CIII) contains Pfam profile: PF00011 Hsp20/alpha crystallin family; identified as |
| Repression | class CIII in Scharf, K. D., et al, Cell Stress & Chaperones (2001) 6: 225-237. (68414.m06159) | ||||
| 119 | (E) Internal/External Glu | NC-I-NC | 262831_at | At1g14730 | cytochrome B561 family similar to cytochrome GB: AAD11424 GI: 4206110 [Mesembryanthemum crystallinum]; contains Pfam |
| Repression | domain, PF03188: Cytochrome b561 (68414.m01761) | ||||
| 120 | (E) Internal/External Glu | NC-I-NC | 262341_at | At1g64230 | ubiquitin-conjugating enzyme, putative identical or nearly so to Ubiquitin-conjugating enzymes SP|P35132, SP|P35131, SP|P35133 |
| Repression | from {Arabidopsis thaliana}; contains Pfam profile PF00179: Ubiquitin-conjugating enzyme (68414.m07276) | ||||
| 121 | (E) Internal/External Glu | NC-I-NC | 262235_at | At1g48350 | ribosomal protein L18 family protein similar to ribosomal protein L18 GI: 3980238 from [Thermotoga maritima] (68414.m05401) |
| Repression | |||||
| 122 | (E) Internal/External Glu | NC-I-NC | 262092_at | At1g56150 | auxin-responsive family protein similar to SP: P33082 Auxin-induced protein X15. [Soybean] (Glycine max) (68414.m06450) |
| Repression | |||||
| 123 | (E) Internal/External Glu | NC-I-NC | 261564_at | At1g01720 | no apical meristem (NAM) family protein contains Pfam PF02365: No apical meristem (NAM) domain; similar to NAC domain |
| Repression | protein NAM GB: AAD17313 GI: 4325282 from [Arabidopsis thaliana] (68414.m00090) | ||||
| 124 | (E) Internal/External Glu | NC-I-NC | 261484_at | At1g14400 | ubiquitin-conjugating enzyme 1 (UBC1) E2; identical to gi: 431259, SP: P25865 (68414.m01707) |
| Repression | |||||
| 125 | (E) Internal/External Glu | NC-I-NC | 261445_at | At1g28380 | expressed protein (68414.m03487) |
| Repression | |||||
| 126 | (E) Internal/External Glu | NC-I-NC | 261143_at | At1g19770 | purine permease-related low similarity to purine permease [Arabidopsis thaliana] GI: 7620007; contains Pfam profile PF03151: |
| Repression | Domain of unknown function, DUF250 (68414.m02471) | ||||
| 127 | (E) Internal/External Glu | NC-I-NC | 261081_at | At1g07350 | transformer serine/arginine-rich ribonucleoprotein, putative similar to GB: Y09506 from [Nicotiana tabacum] (Plant Mol. Biol. 35 (3), |
| Repression | 261-269 (1997)) (68414.m00783) | ||||
| 128 | (E) Internal/External Glu | NC-I-NC | 260831_at | At1g06830 | glutaredoxin family protein contains INTERPRO Domain IPR002109, Glutaredoxin (thioltransferase) (68414.m00728) |
| Repression | |||||
| 129 | (E) Internal/External Glu | NC-I-NC | 260481_at | At1g10960 | ferredoxin, chloroplast, putative strong similarity to FERREDOXIN PRECURSOR GB: P16972 [SP|P16972] from [Arabidopsis |
| Repression | thaliana] (68414.m01258) | ||||
| 130 | (E) Internal/External Glu | NC-I-NC | 260180_at | At1g70660 | ubiquitin-conjugating enzyme family protein similar to TRAF6-regulated IKK activator 1 beta Uev1A [Homo sapiens] GI: 10880969; |
| Repression | contains Pfam profile PF00179: Ubiquitin-conjugating enzyme (68414.m08146) | ||||
| 131 | (E) Internal/External Glu | NC-I-NC | 259518_at | At1g20510 | 4-coumarate-CoA ligase family protein/4-coumaroyl-CoA synthase family protein similar to SP|P14912 and SP|P14913 from |
| Repression | Petroselinum crispum: contains Pfam AMP-binding enzyme domain PF00501 (68414.m02555) | ||||
| 132 | (E) Internal/External Glu | NC-I-NC | 259312_at | At3g05200 | zinc finger (C3HC4-type RING finger) family protein (ATL6) contains Pfam profile: PF00097: Zinc finger, C3HC4 type (RING finger) |
| Repression | (68416.m00567) | ||||
| 133 | (E) Internal/External Glu | NC-I-NC | 259249_at | At3g07790 | DGCR14-related similar to DGCR14 protein (DiGeorge syndrome critical region 14) (ES2 protein) (Swiss-Prot: Q96DF8) [Homo |
| Repression | sapiens] (68416.m00951) | ||||
| 134 | (E) Internal/External Glu | NC-I-NC | 258897_at | At3g05730 | expressed protein (68416.m00642) |
| Repression | |||||
| 135 | (E) Internal/External Glu | NC-I-NC | 258787_al | At3g11840 | U-box domain-containing protein low similarity to immediate-early fungal elicitor protein CMPG1 [Petroselinum crispum] |
| Repression | GI: 14582200; contains Pfam profile PF04564: U-box domain (68416.m01451) | ||||
| 136 | (E) Internal/External Glu | NC-I-NC | 258452_at | At3g22370 | alternative oxidase 1a, mitochondrial (AOX1A) identical to GB: Q39219 [SP|Q39219] from [Arabidopsis thaliana] (68416.m02824) |
| Repression | |||||
| 137 | (E) Internal/External Glu | NC-I-NC | 257700_at | At3g12740 | LEM3 (ligand-effect modulator 3) family protein/CDC50 family protein Similar to GI: 4585976; GI: 4966357; GI: 4835763; |
| Repression | GI: 9757735 from [Arabidopsis thaliana] (68416.m01591) | ||||
| 138 | (E) Internal/External Glu | NC-I-NC | 256663_at | At3g12050 | Aha1 domain-containing protein contains Pfam PF05146: Aha1 domain; similar to Protein C14orf3 (HSPC322) (Swiss- |
| Repression | Prot: O95433) [Homo sapiens] (68416.m01496) | ||||
| 139 | (E) Internal/External Glu | NC-I-NC | 256178_s_at | At1g51780 | IAA-amino acid hydrolase 5/auxin conjugate hydrolase (ILL5) identical to auxin conjugate hydrolase ILL5 [Arabidopsis thaliana] |
| Repression | gi|5725649|gb|AAD48152; contains nonconsensus AT acceptor splice site at exon3 (68414.m05835) | ||||
| 140 | (E) Internal/External Glu | NC-I-NC | 256046_at | At1g07135 | glycine-rich protein (68414.m00759) |
| Repression | |||||
| 141 | (E) Internal/External Glu | NC-I-NC | 254861_at | At4g12040 | zinc finger (AN1-like) family protein contains Pfam domains, PF01428: AN1-like Zinc finger and PF01754: A20-like zinc finger |
| Repression | (68417.m01915) | ||||
| 142 | (E) Internal/External Glu | NC-I-NC | 254331_s_at | At4g22710 | cytochrome P450 family protein contains Pfam profile: PF00067 cytochrome p450 (68417.m03276) |
| Repression | |||||
| 143 | (E) Internal/External Glu | NC-I-NC | 254231_at | At4g23810 | WRKY family transcription factor AR411 - Arabidopsis thaliana (thale cress), PID: g1669603 (68417.m03423) |
| Repression | |||||
| 144 | (E) Internal/External Glu | NC-I-NC | 254120_at | At4g24570 | mitochondrial substrate carrier family protein contains Pfam profile: PF00153 mitochondrial carrier protein (68417.m03521) |
| Repression | |||||
| 145 | (E) Internal/External Glu | NC-I-NC | 253664_at | At4g30210 | NADPH-cytochrome p450 reductase, putative/NADPH-ferrihemoprotein reductase, putative similar to NADPH-cytochrome P450 |
| Repression | oxydoreductase from (Populus balsamifera subsp. trichocarpa x Populus deltoides] GI: 13183564, GI: 13183566 (68417.m04296) | ||||
| 146 | (E) Internal/External Glu | NC-I-NC | 253482_at | At4g31985 | 60S ribosomal protein L39 (RPL39C) (68417.m04549) |
| Repression | |||||
| 147 | (E) Internal/External Glu | NC-I-NC | 253273_at | At4g34180 | cyclase family protein contains Pfam profile: PF04199 putative cyclase (68417.m04850) |
| Repression | |||||
| 148 | (E) Internal/External Glu | NC-I-NC | 252422_at | At3g47550 | zinc finger (C3HC4-type RING finger) family protein contains Pfam domain, PF00097: Zinc finger, C3HC4 type (RING finger) |
| Repression | (68416.m05171) | ||||
| 149 | (E) Internal/External Glu | NC-I-NC | 252053_at | At3g52400 | syntaxin, putative (SYP122) similar to SP|Q9ZSD4 Syntaxin 121 (AtSYP121) (Syntaxin-related protein At-Syr1) {Arabidopsis |
| Repression | thaliana} (68416.m05763) | ||||
| 150 | (E) Internal/External Glu | NC-I-NC | 251727_at | At3g56290 | expressed protein (68416.m06257) |
| Repression | |||||
| 151 | (E) Internal/External Glu | NC-I-NC | 249983_at | At5g18470 | curculin-like (mannose-binding) lectin family protein contains Pfam profile: PF01453 lectin (probable mannose binding) |
| Repression | (68418.m02175) | ||||
| 152 | (E) Internal/External Glu | NC-I-NC | 249325_at | At5g40850 | urophorphyrin III methylase (UPM1) identical to urophorphyrin III methylase (GI: 1146165) [Arabidopsis thaliana]; similar to s- |
| Repression | adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase (GI: 1490606) [Arabidopsis thaliana]; similar to Diphthine | ||||
| synthase (Diphtamide biosynthesis methyltransferase) (DPH5) (SP: P32469) [Saccharomyces cerevisiae]; contains Pfam PF00590: | |||||
| Tetrapyrrole (Corrin/Porphyrin) Methylases domain; contains TIGRFAM PF00590: Tetrapyrrole (Corrin/Porphyrin) Methylases | |||||
| (68418.m04960) | |||||
| 153 | (E) Internal/External Glu | NC-I-NC | 249122_at | At5g43850 | acireductone dioxygenase (ARD/ARD′) family protein similar to iron-deficiency induced gene [Hordeum vulgare] GI: 14522834, SIPL |
| Repression | [Homo sapiens] GI: 16551383: contains Pfam profile PF03079: ARD/ARD′ family (68418.m05361) | ||||
| 154 | (E) Internal/External Glu | NC-I-NC | 249078_at | At5g44070 | phytochelatin synthase 1 (PCS1) identical to phytochelatin synthase [Arabidopsis thaliana] gi|18254401|gb|AAL66747; identical to |
| Repression | cDNA phytochelatin synthase, GI: 18254400 (68418.m05392) | ||||
| 155 | (E) Internal/External Glu | NC-I-NC | 248964_at | At5g45340 | cytochrome P450 family protein similar to SP|Q42569|C901_ARATH Cytochrome P450 90A1 (SP: Q42569) [Arabidopsis thaliana]; |
| Repression | contains Pfam profile: PF00067: Cytochrome P450 (68418.m05585) | ||||
| 156 | (E) Internal/External Glu | NC-I-NC | 248799_at | At5g47230 | ethylene-responsive element-binding factor 5 (ERF5) identical to SP|O80341 Ethylene responsive element binding factor 5 |
| Repression | (AtERF5) [Arabidopsis thaliana] (68418.m05824) | ||||
| 157 | (E) Internal/External Glu | NC-I-NC | 248665_at | At5g48855 | zinc finger (C3HC4-type RING finger) family protein contains Pram profile: PF00097 zinc finger, C3HC4 type (RING finger) |
| Repression | (68418.m06017) | ||||
| 158 | (E) Internal/External Glu | NC-I-NC | 248327_at | At5g52750 | heavy-metal-associated domain-containing protein Pfam profile PF00403: Heavy-metal-associated domain (68418.m06547) |
| Repression | |||||
| 159 | (E) Internal/External Glu | NC-I-NC | 247693_at | At5g59730 | exocyst subunit EXO70 family protein leucine zipper-containing protein, Lycopersicon esculentum, PIR: S21495 contains Pfam |
| Repression | domain PF03081: Exo70 exocyst complex subunit; (68418.m07487) | ||||
| 160 | (E) Internal/External Glu | NC-I-NC | 247320_at | At5g64040 | photosystem I reaction center subunit PSI-N, chloroplast, putative/PSI-N, putative (PSAN) SP: P49107; Plant Physicol. 109 (3), |
| Repression | 1126 (1995); similar to SP|P31093 Photosystem I reaction centre subunit N, chloroplast precursor (PSI-N) {Hordeum vulgare} | ||||
| (68418.m08040) | |||||
| 161 | (E) Internal/External Glu | NC-I-NC | 246870_at | At5g26030 | ferrochelatase I identical to Swiss-Prot: P42043 ferrochelatase I, chloroplast/mitochondrial precursor (EC 4.99.1.1) (Protoheme ferro |
| Repression | lyase) (Heme synthetase) [Arabidopsis thaliana] (68418.m03097) | ||||
| 162 | (E) Internal/External Glu | NC-I-NC | 245768_at | At1g33590 | disease resistance protein-related/LRR protein-related contains leucine rich-repeat domains Pfam: PF00580. |
| Repression | INTERPRO: IPR001611; similar to Hcr2-5D [Lycopersicon esculentum] gi|3894393|gb|AAC78596 (68414.m04158) | ||||
| 163 | (E) Internal/External Glu | NC-I-NC | 245247_at | At4g17230 | scarecrow-like transcription factor 13 (SCLL-13) (68417.m02591) |
| Repression | |||||
| 164 | (E) Internal/External Glu | NC-I-NC | 245114_at | At2g41630 | transcription initiation factor IIB-1/general transcription factor TFIIB-1 (TFIIB1) identical to transcription initiation factor IIB-1 |
| Repression | |||||
| (TFIIB1) SP: P48512 from [Arabidopsis thaliana] (68415.m05144) | |||||
| 1 | (F) Internal Glu induction/ | I-NC-D | 254549_at | At4g19880 | glutathione S-transferase-related contains weak hit to Pfam profile PF00043: Glutathione S-transferase, C-terminal domain |
| External Glu repre | (68417.m02914) | ||||
| 2 | (F) Internal Glu repression/ | D-NC-I | 266992_at | At2g39200 | seven transmembrane MLO family protein/MLO-like protein 12 (MLO12) identical to SP|O80961 MLO-like protein 12 (AtMlo12) |
| External Glu ind | {Arabidopsis thaliana}, membrane protein Mlo12 [Arabidopsis thaliana] gi|14091594|gb|AAK53805; similar to MLO protein SWISS- | ||||
| PROT: P93766, NCBI_gi: 1877221 [Hordeum vulgare][Barley] {68415.m04815} | |||||
| 3 | (F) Internal Glu repression/ | D-NC-I | 253277_at | At4g34230 | cinnamyl-alcohol dehydrogenase, putative similar to cinnamyl alcohol dehydrogenase, Nicotiana tabacum [SP|P30359], Populus |
| External Glu ind | deltoides, PATCHX: G288753 (68417.m04864) | ||||
| 1 | Not regulated | NC-NC-NC | 267630_at | At2g42130 | expressed protein contains weak hit to Pfam PF04755: PAP_fibrillin (68415.m05211) |
| 2 | Not regulated | NC-NC-NC | 267638_at | At2g42210 | mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein weak similarity to SP|P25710 NADH- |
| ubiquinone oxidoreductase 21.3 kDa subunit (EC 1.6.5.3) (EC 1.6.99.3) (Neurospora crassa), SP|Q12328 Mitochondrial import | |||||
| inner membrane translocase subunit TIM22 {Saccharomyces cerevisiae}; contains Pfam profile PF02466: Mitochondrial import | |||||
| inner membrane translocase subunit Tim17 (68415.m05224) | |||||
| 3 | Not regulated | NC-NC-NC | 267640_at | At2g32950 | COP1 regulatory protein photomorphogenesis repressor, identical to COP1 regulatory protein/FUSCA protein FUS1 GI: 402685 |
| SP: P43254 (68415.m04039) | |||||
| 4 | Not regulated | NC-NC-NC | 267596_s_at | At2g33050 | leucine-rich repeat family protein contains leucine rich-repeat domains Pfam: PF00560, INTERPR: IPR001611; similar to Hcr2-0B |
| [Lycopersicon esculentum] gi|3894387|gb|AAC78593 (68415.m04053) | |||||
| 5 | Not regulated | NC-NC-NC | 267601_at | At2g32980 | expressed protein (68415.m04042) |
| 6 | Not regulated | NC-NC-NC | 267607_s_at | At2g26740 | epoxide hydrolase, soluble (sEH) identical to ATsEH [Arabidopsis thaliana] GI: 1109600 (68415.m03207) |
| 7 | Not regulated | NC-NC-NC | 287608_at | At2g26770 | plectin-related contains weak similarity to Swiss-Prot: Q9JI55 plectin 1 (PLTN, PCN, 300-kDa intermediate filament-associated |
| protein, IFAP300)[Cricetulus griseus] (68415.m03210) | |||||
| 8 | Not regulated | NC-NC-NC | 267609_at | At2g26780 | expressed protein contains Pfam profile TBP (TATA-binding protein) -interacting protein 120 (TIP120); contains TIGRFAM profile |
| TIGR01612: reticulocyte binding protein (68415.m03212) | |||||
| 9 | Not regulated | NC-NC-NC | 267610_at | At2g26650 | potassium channel protein 1 (AKT1) identical to AKT1 [Arabidopsis thaliana] gi|563112|gb|AAA96810; member of the 1 pore, 6 |
| transmembrane (1P/6TM-Shaker-type) K+ channel family, PMID: 11500563 (68415.m03197) | |||||
| 10 | Not regulated | NC-NC-NC | 267617_at | At2g26670 | heme oxygenase 1 (HO1) (HY1) identical to plastid heme oxygenase (HY1) [Arabidopsis thaliana] GI: 4877362, heme oxygenase 1 |
| [Arabidopsis thaliana] GI: 4530591 GB: AF132475; annotation updated per Seth J. Davis at University of Wisconsin-Madison | |||||
| (68415.m03199) | |||||
| 11 | Not regulated | NC-NC-NC | 267619_at | At2g26730 | leucine-rich repeat transmembrane protein kinase, putative (68415.m03206) |
| 12 | Not regulated | NC-NC-NC | 267624_at | At2g39660 | protein kinase, putative similar to protein kinase gi|166809|gb|AAA18853 (68415.m04864) |
| 13 | Not regulated | NC-NC-NC | 267591_at | At2g39705 | expressed protein (68415.m04871) |
| 14 | Not regulated | NC-NC-NC | 267562_at | At2g39670 | radical SAM domain-containing protein similar to hypothetical protein PIR/S76698/S76698 contains Pfam profile PF04055: radical |
| SAM domain protein (68415.m04866) | |||||
| 15 | Not regulated | NC-NC-NC | 267577_at | At2g30710 | RabGAP/TBC domain-containing protein similar to SP|Q08484 GTPase-activating protein GYP1 {Saccharomyces cerevisiae}; |
| contains Pfam profile PF00566: TBC domain (68415.m03746) | |||||
| 16 | Not regulated | NC-NC-NC | 267580_at | At2g41990 | expressed protein (68415.m05194) |
| 17 | Not regulated | NC-NC-NC | 267583_at | At2g41960 | expressed protein (68415.m05191) |
| 18 | Not regulated | NC-NC-NC | 267586_at | At2g41950 | expressed protein (68415.m05190) |
| 19 | Not regulated | NC-NC-NC | 267533_at | At2g42070 | MutT/nudix family protein similar to SP|Q58549 ADP-ribose pyrophosphatase (EC 3.6.1.13) {Methanococcus jannaschi}; contains |
| Pfam profile PF00293: NUDIX domain (68415.m05202) | |||||
| 20 | Not regulated | NC-NC-NC | 267535_at | At2g41940 | zinc finger (C2H2 type) family protein contains Pfam domain, PF00096: Zinc finger, C2H2 type (68415.m05188) |
| 21 | Not regulated | NC-NC-NC | 267539_at | At2g42030 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) |
| (68415.m05198) | |||||
| 22 | Not regulated | NC-NC-NC | 267544_at | At2g32720 | cytochrome b5, putative similar to Cytochrome B5 SP: P49098 from [Nicotiana tabacum] (68415.m04004) |
| 23 | Not regulated | NC-NC-NC | 267553_s_at | At2g32650 | expressed protein contains Pfam PF05899: Protein of unknown function (DUF861) (68415.m03988) |
| 24 | Not regulated | NC-NC-NC | 267561_at | At2g45590 | protein kinase family protein contains protein kinase domain, Pfam: PF00069 (68415.m05669) |
| 25 | Not regulated | NC-NC-NC | 267498_at | At2g45720 | armadillo/beta-catenin repeat family protein contains Pfam profile PF00514: Armadillo/beta-catenin-like repeat (68415.m05686) |
| 26 | Not regulated | NC-NC-NC | 267504_at | At2g45530 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) |
| (68415.m05662) | |||||
| 27 | Not regulated | NC-NC-NC | 267506_at | At2g45520 | expressed protein (68415.m05661) |
| 28 | Not regulated | NC-NC-NC | 267507_at | At2g45710 | 40S ribosomal protein S27 (RPS27A) (68415.m05685) |
| 29 | Not regulated | NC-NC-NC | 267510_at | At2g45640 | sin3 associated polypeptide p18 family protein similar to Sin3 associated polypeptide p18 (2HOR0202) (Swiss-Prot: O00422) [Homo |
| sapiens] (68415.m05675) | |||||
| 30 | Not regulated | NC-NC-NC | 267511_at | At2g45670 | calcineurin B subunit-related contains Pfam PF00036: EF hand domain and Prosite PS00018: EF-hand calcium-binding domain: |
| contains Pfam profile PF01553: Acyltransferase; weak similarity to Calcineurin B subunit isoform 2 (Protein phosphatase 2B | |||||
| regulatory subunit 2) (Protein phosphatase 3 regulatory subunit B alpha isoform 2) (Swiss-Prot: Q63811) [Mus musculus] | |||||
| (68415.m05678) | |||||
| 31 | Not regulated | NC-NC-NC | 267513_at | At2g45620 | nucleotidyltransferase family protein low similarity to SP|O13833| Caffeine-induced death protein 1 {Schizosaccharomyces pombe}; |
| contains Pfam profiles PF03828: PAP/25A associated domain, PF01909: Nudeotidyltransferase domain (68415.m05672) | |||||
| 32 | Not regulated | NC-NC-NC | 267515_at | At2g45680 | TCP family transcription factor, putative similar to PCF2 (GI: 2580440) [Oryza sativa] (68415.m05680) |
| 33 | Not regulated | NC-NC-NC | 267517_at | At2g30510 | |
| 34 | Not regulated | NC-NC-NC | 267520_at | At2g30460 | expressed protein contains 4 predicted transmembrane domains; similar to c_pp004044298r (GI: 14597790) [Physcornitrella |
| patens] (68415.m03710) | |||||
| 35 | Not regulated | NC-NC-NC | 267526_at | At2g30570 | photosystem II reaction center W (PsbW) protein-related similar to photosystem II reaction center W protein SP: Q41387 from |
| [Spinacia oleracea] (68415.m03723) | |||||
| 36 | Not regulated | NC-NC-NC | 267472_at | At2g02850 | plastocyanin-like domain-containing protein/plantacyanin, putative similar to plantacyanin GI: 3395754 from [Spinacia oleracea] |
| (68415.m00234) | |||||
| 37 | Not regulated | NC-NC-NC | 267486_at | At2g02800 | protein kinase (APK2b) identical to protein kinase APK2b [Arabidopsis thaliana] gi|2852449|dbj|BAA24695 (68415.m00224) |
| 38 | Not regulated | NC-NC-NC | 267490_at | At2g19130 | S-locus lectin protein kinase family protein contains Pfam domains PF00954: S-locus glycoprotein family, PF00069: Protein kinase |
| domain and PF01453: Lectin (probable mannose binding) (68415.m02233) | |||||
| 39 | Not regulated | NC-NC-NC | 267442_at | At2g19080 | metaxin-related contains 1 transmembrane domain: similar to Metaxin 1 (component of a preprotein import complex) (Swiss- |
| Prot: P47802) [Mus musculus]; (68415.m02228) | |||||
| 40 | Not regulated | NC-NC-NC | 267448_s_at | At2g33840 | tRNA synthetase class I (W and Y) family protein similar to SP|P54577 Tyrosyl-tRNA synthetase (EC 6.1.1.1) (Tyrosyl-tRNA ligase) |
| (TyrRS) {Homo sapiens}; contains Pfam profile PF00579: tRNA synthetases class I (W and Y) (68415.m04153) | |||||
| 41 | Not regulated | NC-NC-NC | 267448_at | At2g33700 | protein phosphatase 2C, putative/PP2C, putative contains PF00481: Protein phosphatase 2C domain: similar to protein |
| phosphatase-2C (PP2C) (GI: 3643085) [Mesembryanthemum crystallinum] (68415.m04130) | |||||
| 42 | Not regulated | NC-NC-NC | 267459_at | At2g33850 | expressed protein contains 1 transmembrane domain; similar to Protein E6 (Swiss-Prot: Q01197) [Gossypium hirsutum] |
| (68415.m04155) | |||||
| 43 | Not regulated | NC-NC-NC | 267463_at | At2g33845 | DNA-binding protein-related contains weak similarity to G-quartet DNA binding protein 3 [tetrahymena thermophila] |
| gi|4583503|gb|AAD25098 (68415.m04154) | |||||
| 44 | Not regulated | NC-NC-NC | 267435_at | At2g33800 | ribosomal protein S5 family protein contains Pfam profiles PF03719: Ribosomal protein S5, C-terminal domain, PF00333: |
| Ribosomal protein S5, N-terminal domain (68415.m04147) | |||||
| 45 | Not regulated | NC-NC-NC | 267405_at | At2g33740 | copper-binding protein (CUTA) identical to copper-binding protein CUTA GI: 12963361 from [Arabidopsis thaliana], contains Pfam |
| profile: PF03091 CutA1 divalent ion tolerance protein (68415.m04136) | |||||
| 46 | Not regulated | NC-NC-NC | 287413_at | At2g34960 | amino acid permease family protein similar to cationic amino acid transporter 3 [Rattus norvegicus] GI: 2116552; contains Pfam |
| profile PF00324: Amino acid permease (68415.m04290) | |||||
| 47 | Not regulated | NC-NC-NC | 267419_at | At2g35010 | thioredoxin family protein similar to SP|Q42443 Thioredoxin H-type (TRX-H) {Oryza sativa}; contains Pfam profile: PF00085 |
| Thioredoxin (68415.m04295) | |||||
| 48 | Not regulated | NC-NC-NC | 287421_at | At2g35040 | AICARFT/IMPCHase bienzyme family protein similar to SP|P12048 Bifunctional purine biosynthesis protein purH [Includes: |
| Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3) (AICAR transformylase); IMP cyclohydrolase (EC | |||||
| 3.5.4.10) (Inosinicase) (IMP synthetase) (ATIC)] {Bacillus subtilis}; contains Pfam profiles PF01808: AICARFT/IMPCHase | |||||
| bienzyme, PF02142: MGS-like domain (68415.m04299) | |||||
| 49 | Not regulated | NC-NC-NC | 267423_at | At2g35060 | potassium transporter family protein similar to HAK2 [Hordeum vulgare] GI: 7108599, potassium transporter HAK2p |
| [Mesemdyanthemum crystallinum] gi|14091471|gb|AAK53759; KUP/HAK/KT Transporter family member, PMID: 11500563; | |||||
| contains Pfam profile PF02705: K+ potassium transporter (68415.m04301) | |||||
| 50 | Not regulated | NC-NC-NC | 267430_at | At2g34860 | chaperone protein dnaJ-related contains Pfam PF00684: DnaJ central domain (4 repeats); similar to Chaperone protein dnaJ |
| (Heat shock(protein 40) (SP: Q9UXR9) {Methanosarcina thermophila} (68415.m04280) | |||||
| 51 | Not regulated | NC-NC-NC | 287432_at | At2g35020 | UTP-glucose-1-phosphate uridylyltransferase family protein similar to SP|Q16222 UDP-N-acetylhexosamine pyrophosphorytase |
| (Antigen X) {Homo sapiens}; contains Pfam profile PF01704: UTP-glucose-1-phosphate uridylyttransferase (68415.m04296) | |||||
| 52 | Not regulated | NC-NC-NC | 267401_at | At2g26210 | ankyrin repeat family protein contains ankyrin repeats, Pfam: PF00023 (68415.m03147) |
| 53 | Not regulated | NC-NC-NC | 267374_at | At2g26230 | uricase/urate oxidase/nodulin 35, putative identical to uricase SP: O04420 from [Arabidopsis thaliana] (68415.m03149) |
| 54 | Not regulated | NC-NC-NC | 267375_at | At2g28300 | guanine nucleotide binding protein (G-protein) alpha-1 subunit/GP-alpha-1 (GPA1) identical to SP|P18064 Guanine nucleotide- |
| binding protein alpha-1 subunit (GP-alpha-1) {Arabidopsis thaliana} (68415.m03156) | |||||
| 55 | Not regulated | NC-NC-NC | 267377_at | At2g26250 | beta-katoacyl-CoA synthase family (FIDDLEHEAD) (FDH) identical to GB: AJ010713 (fiddlehead protein) (68415.m03151) |
| 56 | Not regulated | NC-NC-NC | 287385_at | At2g44380 | DC1 domain-containing protein highly similar to GP|2435515|AF024504; contains Pfam profile PF03107: DC1 domain |
| (68415.m05520) | |||||
| 57 | Not regulated | NC-NC-NC | 267393_at | At2g44500 | expressed protein contains Pfam PF03138: Plant protein family. The function of this family of plant proteins is unknown; previously |
| annotated as ‘axi 1 protein from Nicotiana tabacum-related’ based on similarity to axi 1 protein (GB: X80301) (GI: 559920) from | |||||
| [Nicotiana tabacum], which, due to scienitific fraud was retracted. Retraction in: Schell J. EMBO J 1999 May 17; 18(10): 2908. | |||||
| PMID: 10400497. (68415.m05532) | |||||
| 58 | Not regulated | NC-NC-NC | 267385_at | At2g44530 | ribose-phosphate pyrophosphokinase, putative/phosphoribosyl diphosphate synthetase, putative very strong similarity to |
| phosphoribosyl pyrophosphate synthase [Spinacia oleracea] GI: 4902849; contains Pfam profile PF00156: Phosphoribosyl | |||||
| transferase domain (88415.m05539) | |||||
| 59 | Not regulated | NC-NC-NC | 267342_at | At2g44520 | UbiA prenyltransferase family protein similar to SP|Q12887 Protoheme IX farnesyltransferase, mitochondrial precursor (EC 2.5.1.—) |
| (Heme O synthase) {Homo sapiens}, SP|P21592 COX10 {Saccharomyces cerevisiae} (68415.m05535) | |||||
| 60 | Not regulated | NC-NC-NC | 267346_at | At2g39940 | coronatine-insensitive 1/COI1 (FBL2) E3 ubiquitin ligase SCF complex F-box subunit; identical to LRR-containing F-box protein |
| GI: 3158394 from [Arabidopsis thaliana] (68415.m04908) | |||||
| 61 | Not regulated | NC-NC-NC | 267348_at | At2g39960 | microsomal signal peptidase 25 kDa subunit, putative (SPC25) identical to Probable microsomal signal peptidase 25 kDa subunit |
| (EC 3.4.—.—) (SPase 25 kDa subunit) (SPC25) (Swiss-Prot: P58684) [Arabidopsis thaliana]; contains non-consensus AT-AC splice | |||||
| sites; contains 1 transmembrane domain; (68415.m04910) | |||||
| 62 | Not regulated | NC-NC-NC | 267358_at | At2g39890 | proline transporter 1 (ProT1) identical to protine transporter 1 GI: 1769901 from [Arabidopsis thaliana] (68415.m04903) |
| 63 | Not regulated | NC-NC-NC | 287360_at | At2g40060 | expressed protein (68415.m04922) |
| 64 | Not regulated | NC-NC-NC | 267363_at | At2g39970 | peroxisomal membrane protein (PMP36) identical to 36 kDa-peroxisornal membrane protein (PMP36) GI: 15146342 from |
| [Arabidopsis thaliana] (68415.m04911) | |||||
| 65 | Not regulated | NC-NC-NC | 267338_at | At2g39990 | eukaryotic translation initiation factor 3 subunit 5/eIF-3 epsilon/eIF3f (TIF3F1) identical to SP|O04202 Eukaryotic translation |
| initiation factor 3 subunit 5 (eIF-3 epsilon) (eIF3 p32 subunit) (eIF3f) {Arabidopsis thaliana}: contains Pfam profile PF01398: | |||||
| Mov34/MPN/PAD-1 family (68415.m04914) | |||||
| 66 | Not regulated | NC-NC-NC | 267312_at | At2g34750 | RNA polymerase I specific transcription initiation factor RRN3 family protein contains Pfam PF05327: RNA polymerase I specific |
| transcription initiation factor RRN3; similar to RRN3 (GI: 7670100) [Homo sapiens] similar to RNA polymerase I specific transcription | |||||
| initiation factor RRN3 (Swiss-Prot: P36070) [Saccharomyces cerevisiae] (68415.m04267) | |||||
| 67 | Not regulated | NC-NC-NC | 267315_at | At2g34720 | CCAAT-binding transcription factor (CBF-B/NF-YA) family protein contains Pfam profile: PF02045 CCAAT-binding transcription |
| factor (CBF-B/NF-YA) subunit B (68415.m04284) | |||||
| 68 | Not regulated | NC-NC-NC | 267323_at | At2g19340 | membrane protein, putative contains 3 transmembrane domains; (68415.m06034) |
| 69 | Not regulated | NC-NC-NC | 267330_at | At2g19270 | expressed protein (68415.m02249) |
| 70 | Not regulated | NC-NC-NC | 287309_at | At2g19385 | expressed protein weak similarity to Cell growth regulating nucleolar protein (Swiss-Prot: Q08288) [Mus musculus] (68415.m02261) |
| 71 | Not regulated | NC-NC-NC | 267278_at | At2g19350 | expressed protein (68415.m02258) |
| 72 | Not regulated | NC-NC-NC | 287280_at | At2g19450 | diacylglycerol O-acyltransferase/acyl CoA: diacylglycerol acyltransferase (DGAT) identical to gi: 5050913, gi: 6625553 |
| (68415.m02272) | |||||
| 73 | Not regulated | NC-NC-NC | 267290_at | At2g23750 | SET domain-containing protein similar to SP|O60018 Cryptic loci regulator 4 (Histone-lysine N-methyltransferase) |
| {Schizosaccharomyces pombe}; contains Pfam profile PF00856: SET domain (68415.m02835) | |||||
| 74 | Not regulated | NC-NC-NC | 267297_at | At2g23780 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (68415.m02840) |
| 75 | Not regulated | NC-NC-NC | 287302_at | At2g30100 | ubiquitin family protein low similarity to SP|Q9UQ13 Leucine-rich repeat protein SHOC-2 (Ras-binding protein Sur-8) {Homo |
| sapiens}; contains Pfam profiles PF00240: Ubiquitin family, PF01535: PPR repeat, PF00560: Leucine Rich Repeat | |||||
| (68415.m03863) | |||||
| 76 | Not regulated | NC-NC-NC | 267306_at | At2g30060 | Ran-binding protein 1b (RanBP1b) nearly identical to atranbp 1b [Arabidopsis thaliana] GI: 2058284 (68415.m03856) |
| 77 | Not regulated | NC-NC-NC | 267307_at | At2g30210 | laccase, putative/diphenol oxidase, putative similar to laccase [Populus balsamifera subsp. trichocarpa][GI: 3805960] |
| (68415.m03674) | |||||
| 78 | Not regulated | NC-NC-NC | 267277_at | At2g30050 | transducin family protein/WD-40 repeat family protein similar to SEC13-related protein (SP: P55735) [Homo sapiens] |
| (68415.m03664) | |||||
| 79 | Not regulated | NC-NC-NC | 287247_at | At2g30170 | expressed protein (68415.m03671) |
| 80 | Not regulated | NC-NC-NC | 267251_at | At2g23070 | casein kinase II alpha chain, putative similar to casein kinase II, alpha chain (CK II) [Zea mays] SWISS-PROT: P28523; contains |
| protein kinase domain, Pfam: PF00069 (68415.m02750) | |||||
| 81 | Not regulated | NC-NC-NC | 267257_at | At2g23080 | casein kinase II alpha chain, putative identical to probable casein kinase II, alpha chain [Arabidopsis thaliana] SWISS- |
| PROT: O64817; similar to casein kinase II, alpha chain 1 [Arabidopsis thaliana] SWISS-PROT: Q08467 (68415.m02751) | |||||
| 82 | Not regulated | NC-NC-NC | 267258_at | At2g23140 | armadillo/beta-catenin repeat family protein/U-box domain-containing protein contains Pfam domain, PF00514: Armadillo/beta- |
| catenin-like repeats and Pfam, PF04564: U-box domain (68415.m02763) | |||||
| 83 | Not regulated | NC-NC-NC | 267260_at | At2g23130 | arabinogalactan-protein (AGP17) identical to gi_11935088_gb_AAG41983 (68415.m02759) |
| 84 | Not regulated | NC-NC-NC | 267262_at | At2g22990 | sinapoylglucose:malate sinapoyltransferase (SNG1) similar to serine carboxypeptidase I precursor (SP: P37890) [Oryza sativa]; |
| contains Pfam profile PF00450: Serine carboxypeptidase; identical to cDNA sinapoylglucose:malate sinapoyltansferase (SNG1) | |||||
| GI: 8699618 (68415.m02733) | |||||
| 85 | Not regulated | NC-NC-NC | 267265_at | At2g22980 | serine carboxypeptidase S10 family protein similar to serine carboxypeptidase I precursor (SP: P37890) from [Oryza sativa] |
| (68415.m02731) | |||||
| 86 | Not regulated | NC-NC-NC | 267266_at | At2g23150 | NRAMP metal ion transporter 3 (NRAMP3) identical to metal transporter Nramp3 [Arabidopsis thaliana] gi|6468012|gb|AAF13278: |
| member of the natural resistance-associated macrophage protein (NRAMP) metal transporter family, PMID: 11500563 | |||||
| (88415.m02765) | |||||
| 87 | Not regulated | NC-NC-NC | 287226_at | At2g44010 | expressed protein (68415.m05472) |
| 88 | Not regulated | NC-NC-NC | 267233_s_at | At2g43920 | thiol methyltransferase, putative similar to thiol methyltransferase 1 GI: 14583119 from [Brassica oleracea] (68415.m05459) |
| 89 | Not regulated | NC-NC-NC | 267238_at | At2g44100 | Rab GDP dissociation inhibitor (GDI1) identical to GDP dissociation inhibitor [Arabidopsis thaliana] GI: 1655424 (68415.m05484) |
| 90 | Not regulated | NC-NC-NC | 267211_at | At2g44065 | ribosomal protein L2 family protein similar to ribosomal protein L2 [Gossypium arboreum] GI: 17644114; contains Pfam profile |
| PF03947: Ribosomal Proteins L2. C-terminal domain (68415.m05479) | |||||
| 91 | Not regulated | NC-NC-NC | 267212_at | At2g44060 | late embryogenesis abundant family protein/LEA family protein similar to ethylene-responsive late embryogenesis-like protein |
| [Lycopersicon esculentum] GI: 1684830; contains Pfam profile PF03168: Late embryogenesis abundant protein (68415.m05477) | |||||
| 92 | Not regulated | NC-NC-NC | 267185_at | At2g43950 | expressed protein (68415.m05463) |
| 93 | Not regulated | NC-NC-NC | 267188_at | At2g44050 | 6,7-dimethyl-8-ribityllumazine synthase/DMRL synthase/lumazine synthase/riboflavin synthase identical to 6,7-dimethyl-8- |
| ribityllumazine synthase, chloroplast [precursor] SP: O80575 from [Arabidopsis thaliana] (68415.m05476) | |||||
| 94 | Not regulated | NC-NC-NC | 267189_at | At2g44180 | methionyl aminopeptidase, putative/methionine aminopeptidase, putative/peptidase M, putative similar to SP|P50579 Methionine |
| aminopeptidase 2 (EC 3.4.11.18) (MetAP 2) {Homo sapiens}; contains Pfam profile PF00557: metallopeptidase family M24 | |||||
| (68415.m05496) | |||||
| 95 | Not regulated | NC-NC-NC | 267196_at | At2g30950 | FtsH protease (VAR2) identical to zinc dependent protease VAR2 GI: 7650138 from [Arabidopsis thaliana] (68415.m03775) |
| 96 | Not regulated | NC-NC-NC | 267208_at | At2g30980 | shaggy-related protein kinase delta/ASK-delta/ASK-dzeta (ASK4) identical to shaggy-related protein kinase delta (ASK-delta) |
| (ASK-dzeta) [Arabidopsis thaliana] SWISS-PROT: Q39010 (68415.m03778) | |||||
| 97 | Not regulated | NC-NC-NC | 267151_at | At2g30970 | aspartate aminotransferase, mitochondrial/transaminase A (ASP1) identical to SP|P46643 Aspartate aminotransferase, |
| mitochondrial precursor (EC 2.6.1.1) (Transaminase A) {Arabidopsis thaliana} (68415.m03777) | |||||
| 98 | Not regulated | NC-NC-NC | 267152_at | At2g31040 | ATP synthase protein I-related contains weaks similarity to Swiss-Prot: P08443 ATP synthase protein I [Synechococcus sp.] |
| (68415.m03786) | |||||
| 99 | Not regulated | NC-NC-NC | 267153_at | At2g30860 | glutathione S-transferase, putative identical to GB: Y12295 (68415.m03761) |
| 100 | Not regulated | NC-NC-NC | 267161_at | At2g37680 | phytochrome A specific signal transduction component (PAT3)/far-red elongated hypocotyl protein 1 (FHY1) identical to |
| phytochrome A specific signal transduction component PAT3 [Arabidopsis thaliana] gi|19421998|gb|AAL87850; identical to far-red | |||||
| elongated hypocotyl protein 1 [Arabidopsis thaliana] gi|17148773|gb|AAL35819 (68415.m04621) | |||||
| 101 | Not regulated | NC-NC-NC | 267171_at | At2g37590 | Dof-type zinc finger domain-containing protein (68415.m04612) |
| 102 | Not regulated | NC-NC-NC | 267175_s_at | At2g37620 | actin 1 (ACT1) identical to SP|P10671 Actin 1 (Actin 3) {Arabidopsis thaliana} (68415.m04615) |
| 103 | Not regulated | NC-NC-NC | 267179_at | At2g37550 | arabidopsis pde1 suppressor 1 protein (ASP1) identical to arabidopsis pde1 suppressor 1 (Asp1) from GI: 4519792 [Arabidopsis |
| thaliana]; contains InterPro accession IPR001164: Human Rev interacting-like protein (hRIP) (68415.m04605) | |||||
| 104 | Not regulated | NC-NC-NC | 267180_at | At2g37570 | expressed protein (68415.m04608) |
| 105 | Not regulated | NC-NC-NC | 267181_at | At2g37760 | aldo/keto reductase family protein similar to chalcone reductase [Sesbania rostrata][GI: 2792155], and aldose reductase ALDRXV4 |
| [Xerophyte viscosa][GI: 4539944], [Hordeum vulgare][GI: 728592] (68415.m04633) | |||||
| 106 | Not regulated | NC-NC-NC | 267121_at | At2g23540 | GDSL-motif lipase/hydrolase family protein similar to family II lipase EXL1 GI: 15054382 from [Arabidopsis thaliana]; contains Pfam |
| profile PF00657: GDSL-like Lipase/Acylhydrolase (68415.m02809) | |||||
| 107 | Not regulated | NC-NC-NC | 267127_at | At2g23610 | esterase, putative similar to ethylene-induced esterase [Citrus sinensis] GI: 14279437, polyneuridine aldehyde esterase [Rauvolfia |
| serpentina] GI: 6651393; contains Pfam profile PF00561: hydrolase, alpha/beta fold family (68415.m02817) | |||||
| 108 | Not regulated | NC-NC-NC | 267128_at | At2g23620 | esterase, putative similar to ethylene-induced esterase [Citrus sinensis] GI: 14279437, polyneuridine aldehyde esterase [Rauvolfia |
| serpentina] GI: 6651393; contains Pfam profile PF00561: hydrolase, alpha/beta fold family (68415.m02818) | |||||
| 109 | Not regulated | NC-NC-NC | 267132_at | At2g23420 | nicotinate phosphoribosyltransferase family protein/NAPRTase family protein contains Pfam domain PF04095: Nicotinate |
| phosphoribosyltransferase (NAPRTase) (68415.m02796) | |||||
| 110 | Not regulated | NC-NC-NC | 267134_at | At2g23450 | protein kinase family protein contains protein kinase domain, Pfam: PF00069 (68415.m02799) |
| 111 | Not regulated | NC-NC-NC | 267142_at | At2g38290 | ammonium transporter 2 (AMT2) identical to SP|Q9M6N7 Ammonium transporter 2 (AtAMT2) {Arabidopsis thaliana} |
| (68415.m04703) | |||||
| 112 | Not regulated | NC-NC-NC | 267145_at | At2g38130 | GCN5-related N-acetyltransferase, putative similar to SP|Q03503 L-A virus GAG protein N-acetyltransferase (EC 2.3.1.—) |
| {Saccharomyces cerevisiae}; contains Pfam profile PF00583: acetyltransferase, GNAT family (68415.m04680) | |||||
| 113 | Not regulated | NC-NC-NC | 267088_at | At2g38140 | chloroplast 30S ribosomal protein S31 (PSRP4) (68415.m04682) |
| 114 | Not regulated | NC-NC-NC | 267090_at | At2g38270 | CAX-interacting protein, putative identical to cDNA CAXIP1-like protein GI: 27752306; contains Pfam profile PF00462: Glutaredoxin; |
| contains TIGRfam profile TIGR00365: glutaredoxin-related protein (68415.m04700) | |||||
| 115 | Not regulated | NC-NC-NC | 267092_at | At2g38120 | amino acid permease, putative (AUX1) identical to AUX1 GI: 1531758 from [Arabidopsis thaliana] (68415.m04679) |
| 116 | Not regulated | NC-NC-NC | 267093_at | At2g38170 | calcium exchanger (CAX1) identical to high affinity calcium antiporter CAX1 [Arabidopsis thaliana] gi|9256741|gb|AAB05913, |
| except a possible frameshift at base 58008. Sequence has been confirmed with 5 sequencing reads.; Ca2+: Cation Antiporter | |||||
| (CaCA) Family member PMID: 11500563 (68415.m04685) | |||||
| 117 | Not regulated | NC-NC-NC | 267094_at | At2g38080 | laccase, putative/diphenol oxidase, putative similar to diphenol oxidase [Nicotiana tabacum][GI: 1685087] (68415.m04674) |
| 118 | Not regulated | NC-NC-NC | 267095_at | At2g38280 | AMP deaminase, putative/myoadenylate deaminase, putative similar to SP|P15274 AMP deaminase (EC 3.5.4.6) (Myoadenylate |
| deaminase) {Saccharomyces cerevisiae}; contains Pfam profile PF00962: Adenosine/AMP deaminase (68415.m04701) | |||||
| 119 | Not regulated | NC-NC-NC | 267096_at | At2g38180 | GDSL-motif lipase/hydrolase family protein similar to SP|P41734 Isoamyl acetate-hydrolyzing esterase (EC 3.1.—.—) {Saccharomyces |
| cerevisiae}; contains Pfam profile PF00657: Lipase/Acylhydrolase with GDSL-like motif (68415.m04688) | |||||
| 120 | Not regulated | NC-NC-NC | 267102_at | At2g41500 | WD-40 repeat family protein/small nuclear ribonucleoprotein Prp4p-related similar to U4/U6 small nuclear ribonucleoprotein hPrp4 |
| (GP: 2708305) {Homo sapiens}; contains Pfam PF00400: WD domain, G-beta repeat (7 | |||||
| copies)|19877698|gb|AU238529.1|AU238529 (68415.m05127) | |||||
| 121 | Not regulated | NC-NC-NC | 267106_s_at | At2g14740 | vacuolar sorting receptor, putative nearly identical to vacuolar sorting receptor homolog [Arabidopsis thaliana] GI: 1737220; contains |
| a calcium-binding EGF-like domain signature (68415.m01662) | |||||
| 122 | Not regulated | NC-NC-NC | 267057_at | At2g32500 | expressed protein (68415.m03971) |
| 123 | Not regulated | NC-NC-NC | 267059_at | At2g32520 | dienelactone hydrolase family protein low similarity to dienelactone hydrolase [Pseudomonas resinovorans] GI: 13094163; contains |
| Pfam profile PF01738: Dienelactone hydrolase family (68415.m03973) | |||||
| 124 | Not regulated | NC-NC-NC | 267062_at | At2g32600 | hydroxyproline-rich glycoprotein family protein similar to SWISS-PROT: Q15428 (68415.m03980) |
| 125 | Not regulated | NC-NC-NC | 267063_at | At2g41120 | expressed protein (68415.m05079) |
| 126 | Not regulated | NC-NC-NC | 267064_at | At2g41110 | calmodulin-2/3/5 (CAM2) (CAL1) almost identical to Calmodulin-2/3/5 SP: P25069 from [Arabidopsis thaliana] (68415.m05078) |
| 127 | Not regulated | NC-NC-NC | 267069_at | At2g41010 | VQ motif-containing protein contains PF05678: VQ motif (68415.m05065) |
| 128 | Not regulated | NC-NC-NC | 267073_at | At2g41160 | ubiquitin-associated (UBA)/TS-N domain-containing protein contains Pfam profile PF00627: UBA/TS-N domain (68415.m05084) |
| 129 | Not regulated | NC-NC-NC | 267077_at | At2g40970 | myb family transcription factor contains Pfam profile: PF00249 myb-like DNA-binding domain (68415.m05060) |
| 130 | Not regulated | NC-NC-NC | 267080_at | At2g41190 | amino acid transporter family protein low similarity to vesicular GABA transporter [Rattus norvegicus] GI: 2587061; belongs to |
| INTERPRO: IPR002422 amino acid/polyamine transporter, family II (68415.m05087) | |||||
| 131 | Not regulated | NC-NC-NC | 267084_at | At2g41180 | sigA-binding protein-related low similarity to SigA binding protein [Arabidopsis thaliana] GI: 6980074; contains Pfam domain |
| PF05678: VQ motif (68415.m05086) | |||||
| 132 | Not regulated | NC-NC-NC | 267052_at | At2g38410 | VHS domain-containing protein/GAT domain-containing protein weak similarity to hepatocyte growth factor-regulated tyrosine |
| kinase substrate HRS isoform 2 [Homo sapiens] GI: 9022389; contains Pfam profiles PF00790: VHS domain, PF03127: GAT | |||||
| domain (68415.m04718) | |||||
| 133 | Not regulated | NC-NC-NC | 267027_at | At2g38330 | MATE efflux family protein low similarity to enhanced disease susceptibility 5 [Arabidopsis thaliana] GI: 16589070; contains Pfam |
| profile PF01554: Uncharacterized membrane protein family (68415.m04709) | |||||
| 134 | Not regulated | NC-NC-NC | 267033_at | At2g38450 | expressed protein (68415.m04722) |
| 135 | Not regulated | NC-NC-NC | 266996_at | At2g34490 | cytochrome P450 family protein similar to Cytochrome P450 61 (C-22 sterol desaturase) (SP: P54781) {Saccharomyces cerevisiae}; |
| contains Pfam profile: PF00067 cytochrome P450; supported by full-length cDNA: Ceres: 158108, (68415.m04235) | |||||
| 136 | Not regulated | NC-NC-NC | 267000_at | At2g34310 | expressed protein (68415.m04197) |
| 137 | Not regulated | NC-NC-NC | 267002_s_at | At2g34430 | chlorophyll A-B binding protein/LHCII type I (LHB1B1) identical to photosystem II type I chlorophyll a/b binding protein [Arabidopsis |
| thaliana] GI: 16366 (68415.m04223) | |||||
| 138 | Not regulated | NC-NC-NC | 267004_at | At2g34260 | transducin family protein/WD-40 repeat family protein similar to Vegetatible incompatibility protein HET-E-1 (SP: Q00808) |
| {Podospora anserina} (68415.m04191) | |||||
| 139 | Not regulated | NC-NC-NC | 266988_at | At2g39310 | jacalin lectin family protein similar to myrosinase-binding protein homolog [Arabidopsis thaliana] GI: 2997767; contains Pfam profile |
| PF01419 jacalin-like lectin domain (68415.m04826) | |||||
| 140 | Not regulated | NC-NC-NC | 266991_at | At2g39290 | phosphatidylglycerolphosphate synthase (PGS1) identical to phosphatidylglycerolphosphate synthase GI: 13365519 from |
| [Arabidopsis thaliana] (68415.m04824) | |||||
| 141 | Not regulated | NC-NC-NC | 266963_at | At2g39450 | cation efflux family protein contains cation efflux family protein domain, Pfam: PF01545 (68415.m04842) |
| 142 | Not regulated | NC-NC-NC | 266975_at | At2g39380 | exocyst subunit EXO70 family protein contains Pfam domain PF03081: Exo70 exocyst complex subunit; (68415.m04833) |
| 143 | Not regulated | NC-NC-NC | 266977_at | At2g39420 | esterase/lipase/thioesterase family protein similar to monoglyceride lipase from [Homo sapiens] GI: 14594904, [Mus musculus] |
| GI: 2632162; contains Interpro entry IPR000379 (68415.m04839) | |||||
| 144 | Not regulated | NC-NC-NC | 266978_at | At2g39430 | disease resistance-responsive protein-related/dirigent protein-related contains similarity to disease resistance response protein |
| 206-d [Pisum sativum] gi|508844|gb|AAB18669; contains similarity to dirigent protein [Thuja plicata] gi|6694709|gb|AAF25365 | |||||
| (68415.m04840) | |||||
| 145 | Not regulated | NC-NC-NC | 266979_at | At2g39470 | photosystem II reaction center PsbP family protein (68415.m04844) |
| 146 | Not regulated | NC-NC-NC | 266938_at | At2g18950 | homogentisate phytylprenyltransferase family protein (HPT1)/tocopherol phytyltransferase family protein (TPT1) identical to |
| gi: 17104828; contains Pfam profile PF01040: UbiA prenyltransferase family; identical to cDNA tocopherol polyprenyltransferase | |||||
| (TPT1) GI: 17104827 (68415.m02212) | |||||
| 147 | Not regulated | NC-NC-NC | 266948_at | At2g18850 | SET domain-containing protein low similarity to ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit N- |
| methyltransferase I [Spinacia oleracea] GI: 3403236; contains Pfam profile PF00856: SET domain (68415.m02196) | |||||
| 148 | Not regulated | NC-NC-NC | 266950_at | At2g16910 | hydroxyproline-rich glycoprotein family protein (68415.m02206) |
| 149 | Not regulated | NC-NC-NC | 266955_at | At2g34520 | ribosomal protein S14 mitochondrial family protein identical to ribosomal protein S14 {Arabidopsis thaliana} NCBI_gi: 4583554 |
| (68415.m04240) | |||||
| 150 | Not regulated | NC-NC-NC | 266956_at | At2g34510 | expressed protein contains Pfam profile PF04862: Protein of unknown function, DUF642 (68415.m04239) |
| 151 | Not regulated | NC-NC-NC | 266958_at | At2g34630 | geranyl diphosphate synthase, putative/GPPS, putative/dimethylallyltransferase, putative/prenyl transferase, putative identical |
| to GI: 11322965 A. thaliana geranyl diphosphate synthase (68415.m04254) | |||||
| 152 | Not regulated | NC-NC-NC | 266899_at | At2g34620 | mitochondrial transcription termination factor-related/mTERF-related contains Pfam profile PF02536: mTERF (68415.m04253) |
| 153 | Not regulated | NC-NC-NC | 266904_at | At2g34590 | transketolase family protein similar to SP|O66113 Pyruvate dehydrogenase E1 component, beta subunit (EC 1.2.4.1). {Zymomonas |
| mobilis}; contains Pfam profiles PF02779: Transketolase, pyridine binding domain, PF02780: Transketolase, C-terminal domain | |||||
| (68415.m04250) | |||||
| 154 | Not regulated | NC-NC-NC | 266911_at | At2g45910 | protein kinase family protein/U-box domain-containing protein contains Pfam profiles PF00069 Eukaryotic protein kinase domain, |
| PF04564: U-box domain; supported by tandem duplication of (GI: 3386604) (TIGR_Ath1: At2g45920) [Arabidopsis thaliana] | |||||
| (68415.m05709) | |||||
| 155 | Not regulated | NC-NC-NC | 266924_at | At2g45730 | eukaryotic initiation factor 3 gamma subunit family protein contains Pfam profile PF04189: Eukaryotic initiation factor 3, gamma |
| subunit (68415.m05688) | |||||
| 156 | Not regulated | NC-NC-NC | 266896_at | At2g45810 | DEAD/DEAH box helicase, putative (68415.m05697) |
| 157 | Not regulated | NC-NC-NC | 266898_at | At2g45990 | expressed protein (68415.m05718) |
| 158 | Not regulated | NC-NC-NC | 266876_at | At2g44820 | expressed protein (68415.m05578) |
| 159 | Not regulated | NC-NC-NC | 266885_at | At2g44610 | Ras-related GTP-binding protein, putative similar to GTP-binding protein GI: 623586 from [Nicotiana tabacum]; contains an ADP- |
| ribosylation factors family signature for proteins involved in protein trafficking (68415.m05553) | |||||
| 160 | Not regulated | NC-NC-NC | 266887_at | At2g44650 | chloroplast chaperonin 10 (cpn10) identical to chloroplast chaperonin 10 GI: 14041813 from [Arabidopsis thaliana] (68415.m05557) |
| 161 | Not regulated | NC-NC-NC | 266889_at | At2g44640 | expressed protein (68415.m05556) |
| 162 | Not regulated | NC-NC-NC | 266890_at | At2g44620 | acyl carrier protein, mitochondrial/ACP/NADH-ubiquinone oxidoreductase 9.6 kDa subunit identical to SP|P53665 Acyl carrier |
| protein, mitochondrial precursor (ACP) (NADH-ubiquinone oxidoreductase 9.6 kDa subunit) (MtACP-1) {Arabidopsis thaliana}; | |||||
| identical to cDNA acyl carrier protein precursor GI: 468285 (68415.m05554) | |||||
| 163 | Not regulated | NC-NC-NC | 266838_at | At2g25980 | jacalin lectin family protein similar to myrosinase-binding protein homolog [Arabidopsis thaliana] GI: 2997767; contains Pfam profile |
| PF01419 jacalin-like lectin domain (68415.m03120) | |||||
| 164 | Not regulated | NC-NC-NC | 266845_at | At2g26110 | expressed protein (68415.m03133) |
| 165 | Not regulated | NC-NC-NC | 266846_at | At2g25970 | KH domain-containing protein (68415.m03117) |
| 166 | Not regulated | NC-NC-NC | 266847_at | At2g26060 | transducin family protein/WD-40 repeat family protein contains 7 WD-40 repeats (PF00400); similar to WD40-repeat containing |
| protein Ciao 1 (SP: O76071) [Homo sapiens] (68415.m03129) | |||||
| 167 | Not regulated | NC-NC-NC | 266848_at | At2g25950 | expressed protein (68415.m03115) |
| 168 | Not regulated | NC-NC-NC | 266855_at | At2g26920 | ubiquitin-associated (UBA)/TS-N domain-containing protein contains Pfam profile PF00627; UBA/TS-N domain (68415.m03229) |
| 169 | Not regulated | NC-NC-NC | 266861_at | At2g26830 | choline/ethanolamine kinase family protein similar to SP|Q9HBU6 Ethanolamine kinase (EC 2.7.1.82) (EKI){Homo sapiens}, |
| GmCK3p [Glycine max] GI: 1438883; contains Pfam profile PF01633: Choline/ethanolamine kinase (68415.m03219) | |||||
| 170 | Not regulated | NC-NC-NC | 266863_at | At2g26930 | 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (CMK) (CDPMEK) identical to 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, |
| chloroplast precursor (CMK) (4-(cytidine-5′-diphospho)-2-C-methyl-D-erythritol kinase)(CDPMEK) [Arabidopsis thaliana] SWISS- | |||||
| PROT: O81014 (68415.m03230) | |||||
| 171 | Not regulated | NC-NC-NC | 266865_at | At2g29980 | omega-3 fatty acid desaturase, endoplasmic reticulum (FAD3) identical to SP: 48623 (68415.m03646) |
| 172 | Not regulated | NC-NC-NC | 266835_at | At2g29990 | pyridine nucleotide-disulphide oxidoreductase family protein similar to SP|P32340 Rotenone-insensitive NADH-ubiquinone |
| oxidoreductase, mitochondrial precursor (EC 1.6.5.3) (Internal NADH dehydrogenase) {Saccharomyces cerevisiae}; contains Pfam | |||||
| profile PF00070: Pyridine nucleotide-disulphide oxidoreductase (68415.m03648) | |||||
| 173 | Not regulated | NC-NC-NC | 266806_at | At2g30000 | expressed protein contains Pfam domain PF03660: Uncharacterised protein family (UPF0123) (68415.m03650) |
| 174 | Not regulated | NC-NC-NC | 266813_at | At2g44920 | thylakoid lumenal 15 kDa protein, chloroplast identical to SP|O22160 Thylakoid lumenal 15 kDa protein, chloroplast precursor (p15) |
| {Arabidopsis thaliana}; contains 8 pentapeptide repeats (68415.m05591) | |||||
| 175 | Not regulated | NC-NC-NC | 266815_at | At2g44900 | armadillo/beta-catenin repeat family protein/F-box family protein contains similarity to F-box protein FBL2 GI: 6010699 from [Rattus |
| norvegicus]; contains Pfam profiles PF00514: Armadillo/beta-catenin-like repeat, PF00646: F-box domain (68415.m05589) | |||||
| 176 | Not regulated | NC-NC-NC | 266819_at | At2g44870 | expressed protein (68415.m05586) |
| 177 | Not regulated | NC-NC-NC | 266799_at | At2g22860 | phytosulfokines 2 (PSK2) identical to phytosulfokines 2 (PSK2) from [Arabidopsis thaliana] (68415.m02714) |
| 178 | Not regulated | NC-NC-NC | 266790_at | At2g28950 | expansin, putative (EXP6) similar to expansin GI: 2828241 from [Brassica napus]; contains Pfam profile PF01357: Pollen allergen |
| (68415.m03521) | |||||
| 179 | Not regulated | NC-NC-NC | 266791_at | At2g02960 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile PF00097: Zinc finger, C3HC4 type (RING finger); |
| contains PROSITE PS00190: Cytochrome c family heme-binding site signature (68415.m00244) | |||||
| 180 | Not regulated | NC-NC-NC | 266792_at | At2g02860 | sucrose transporter/sucrose-proton symporter (SUC3) identical to sucrose transporter [Arabidopsis thaliana] GI: 8052190; similar |
| to sucrose transporters from [Oryza sativa (japonica cultivar-group)] GI: 2723471, [Zea mays] GI: 5771354, [Triticum aestivum] | |||||
| GI: 19548165; contains Pfam profile PF00083: major facilitator superfamily protein (68415.m00235) | |||||
| 181 | Not regulated | NC-NC-NC | 266796_at | At2g02880 | mucin-related similar to putative mucin GI: 18071389 [Oryza sativa] (68415.m00238) |
| 182 | Not regulated | NC-NC-NC | 266770_at | At2g03090 | expansin, putative (EXP15) identical to SWISS-PROT: O80622 alpha-expansin 15 precursor (At-EXP15)[Arabidopsis thaliana]; |
| alpha-expansin gene family, PMID: 11641069 (68415.m00262) | |||||
| 183 | Not regulated | NC-NC-NC | 266744_at | At2g02970 | nucleoside phosphatase family protein/GDA1/CD39 family protein low similarity to SP|P55772 Ectonucleoside triphosphate |
| diphosphohydrolase 1 (EC 3.6.1.5) (Ecto-apyrase) {Mus musculus}; contains Pfam profile PF01150: GDA1/CD39 (nucleoside | |||||
| phosphatase) family (68415.m00249) | |||||
| 184 | Not regulated | NC-NC-NC | 266745_at | At2g02950 | phytochrome kinase substrate 1 (PKS1) identical to Swiss-Prot: Q9SWI1 phytochrome kinase substrate 1 [Arabidopsis thaliana] |
| (68415.m00242) | |||||
| 185 | Not regulated | NC-NC-NC | 266747_at | At2g02870 | kelch repeat-containing F-box family protein weak similarity to Kelch-like protein 5 (Swiss-Prot: Q96PQ7) [Homo sapiens]; contains |
| Pfam profiles PF01344: Kelch motif, PF00646: F-box domain (68415.m00237) | |||||
| 186 | Not regulated | NC-NC-NC | 266758_at | At2g46920 | protein phosphatase 2C family protein/PP2C family protein similar to protein phosphatase-2c (GI: 3608412) [Mesembryanthemum |
| crystallinum]; contains Pfam PF00481: Protein phosphatase 2C domain (68415.m05860) | |||||
| 187 | Not regulated | NC-NC-NC | 266761_at | At2g47130 | short-chain dehydrogenase/reductase (SDR) family protein similar to 3-beta-hydroxysteroiddehydrogenase GI: 15983819 from |
| [Digitalis lanata] (68415.m05886) | |||||
| 188 | Not regulated | NC-NC-NC | 266767_at | At2g46910 | plastid-lipid associated protein PAP/fibrillin family protein contains Pfam profile PF04755: PAP_fibrillin (68415.m05858) |
| 189 | Not regulated | NC-NC-NC | 266768_s_at | At2g47110 | ubiquitin extension protein 6 (UBQ6)/40S ribosomal protein S27A (RPS27aB) identical to GI: 166936 (68415.m05883) |
| 190 | Not regulated | NC-NC-NC | 266735_at | At2g46930 | pectinacetylesterase, putative similar to pectinacetylesterase precursor GI: 1431629 from [Vigna radiata] (68415.m05862) |
| 191 | Not regulated | NC-NC-NC | 266712_at | At2g46750 | FAD-binding domain-containing protein low similarity to SP|P58710 L-gulonolactone oxidase (EC 1.1.3.8) {Mus musculus}; contains |
| Pfam profile PF01565: FAD binding domain (68415.m05833) | |||||
| 192 | Not regulated | NC-NC-NC | 266716_at | At2g46820 | expressed protein (68415.m05842) |
| 193 | Not regulated | NC-NC-NC | 266720_s_at | At2g46790 | pseudo-response regulator 9 (APRR9)/timing of CAB expression 1-like protein (TL1) identical to pseudo-response regulator 9 |
| GI: 10281000 from [Arabidopsis thaliana], timing of CAB expression 1-like protein [Arabidopsis thaliana] GI: 9247022; contains Pfam | |||||
| profile PF00072: Response regulator receiver domain; identical to cDNA timing of CAB expression 1-like protein GI: 9247021 | |||||
| (68415.m05837) | |||||
| 194 | Not regulated | NC-NC-NC | 266679_at | At2g03270 | DNA-binding protein, putative similar to Swiss-Prot: Q60560 DNA-binding protein SMUBP-2 (Immunoglobulin MU binding protein 2) |
| (SMUBP-2) (Insulin II gene enhancer-binding protein)(RIPE3B-binding complex 3B2 P110 subunit) (RIP-1)[Mesocricetus auratus]; | |||||
| identical to putative helicase (atpc-2 gene) cDNA NCBI_gi: 11191230 (68415.m00280) | |||||
| 195 | Not regulated | NC-NC-NC | 266683_at | At2g19950 | expressed protein contains 2 transmembrane domains; weak similarity to HPSR2-heavy chain potential motor protein (GI: 871048) |
| [Giardia intestinalis] (68415.m02332) | |||||
| 196 | Not regulated | NC-NC-NC | 266685_at | At2g19710 | expressed protein contains Pfam profile: PF03398 eukaryotic protein of unknown function, DUF292 (68415.m02303) |
| 197 | Not regulated | NC-NC-NC | 266687_at | At2g19670 | protein arginine N-methyltransferase, putative similar to protein arginine N-methyltransferase 1-variant 1 [Homo sapiens] |
| GI: 7453577 (68415.m02299) | |||||
| 198 | Not regulated | NC-NC-NC | 266694_at | At2g19790 | clathrin adaptor complex small chain family protein contains Pfam profile: PF01217 clathrin adaptor complex small chain |
| (68415.m02312) | |||||
| 199 | Not regulated | NC-NC-NC | 266695_at | At2g19810 | zinc finger (CCCH-type) family protein contains Pfam domain, PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and similar) |
| (68415.m02316) | |||||
| 200 | Not regulated | NC-NC-NC | 266696_at | At2g19680 | mitochondrial ATP synthase g subunit family protein contains Pfam profile: PF04718 mitochondrial ATP synthase g subunit |
| (68415.m02300) | |||||
| 201 | Not regulated | NC-NC-NC | 266698_at | At2g19830 | SNF7 family protein contains Pfam domain, PF03357: SNF7 family (68415.m02319) |
| 202 | Not regulated | NC-NC-NC | 266699_at | At2g19730 | 60S ribosomal protein L28 (RPL28A) (68415.m02305) |
| 203 | Not regulated | NC-NC-NC | 266700_at | At2g19740 | 60S ribosomal protein L31 (RPL31A) (68415.m02306) |
| 204 | Not regulated | NC-NC-NC | 266701_at | At2g19760 | profilin 1 (PRO1) (PFN1) (PRF1)/allergen Ara t 8 identical to profilin 1 (Allergen Ara t 8) SP: Q42449 GI: 1353770 from [Arabidopsis |
| thaliana] (68415.m02309) | |||||
| 205 | Not regulated | NC-NC-NC | 266702_at | At2g19860 | hexokinase 2 (HXK2) identical to hexokinase 2 [Arabidopsis thaliana] Swiss-Prot: P93834 (68415.m02322) |
| 206 | Not regulated | NC-NC-NC | 266704_at | At2g19940 | semialdehyde dehydrogenase family protein similar to N-acetyl-glutamyl-phosphate reductase [Campylobacter jejuni] GI: 6650362; |
| contains Pfam profiles PF02774: Semialdehyde dehydrogenase dimerisation domain, PF01118: Semialdehyde dehydrogenase | |||||
| NAD binding domain (68415.m02330) | |||||
| 207 | Not regulated | NC-NC-NC | 266705_at | At2g19750 | 40S ribosomal protein S30 (RPS30A) (68415.m02307) |
| 208 | Not regulated | NC-NC-NC | 266678_at | At2g25870 | haloacid dehalogenase-like hydrolase family protein contains Pfam profiles PF00702: haloacid dehalogenase-like hydrolase, |
| PF02130: Uncharacterized protein family UPF0054 (68415.m03105) | |||||
| 209 | Not regulated | NC-NC-NC | 266648_at | At2g25840 | tRNA synthetase class I (W and Y) family protein contains Pfam profile: PF00579 tRNA synthetases class I (W and Y) |
| (68415.m03100) | |||||
| 210 | Not regulated | NC-NC-NC | 266649_at | At2g25810 | tonoplast intrinsic protein, putative similar to tonoplast intrinsic protein GI: 4584429 from [Nicotiana tabacum] (68415.m03097) |
| 211 | Not regulated | NC-NC-NC | 266652_at | At2g25750 | |
| 212 | Not regulated | NC-NC-NC | 266657_at | At2g25910 | 3′-5′ exonuclease domain-containing protein/K homology domain-containing protein/KH domain-containing protein contains Pfam |
| profiles PF01612: 3′-5′ exonuclease, PF00013: KH domain (68415.m03109) | |||||
| 213 | Not regulated | NC-NC-NC | 266660_at | At2g25920 | expressed protein (68415.m03110) |
| 214 | Not regulated | NC-NC-NC | 266672_at | At2g29650 | inorganic phosphate transporter, putative similar to brain specific Na+-dependent inorganic phosphate cotransporter [Rattus |
| norvegicus] GI: 507415; contains Pfam profile PF00083: major facilitator superfamily protein (68415.m03602) | |||||
| 215 | Not regulated | NC-NC-NC | 266673_at | At2g29630 | thiamine biosynthesis family protein/thiC family protein contains Pfam profile: PF01964 ThiC family (68415.m03599) |
| 216 | Not regulated | NC-NC-NC | 266646_at | At2g29700 | pleckstrin homology (PH) domain-containing protein (PH1) identical to AtPH1 [Arabidopsis thaliana] GI: 5926716; contains Pfam |
| profile PF00169: PH domain (68415.m03610) | |||||
| 217 | Not regulated | NC-NC-NC | 266624_s_at | At2g35390 | ribose-phosphate pyrophosphokinase 1/phosphoribosyl diphosphate synthetase 1 (PRSI) identical to phosphoribosyl diphosphate |
| synthetase 1 (ribose-phosphate pyrophosphokinase 1 (PRS I) [Arabidopsis thaliana] GI: 633140, SP|Q42581 (68415.m04338) | |||||
| 218 | Not regulated | NC-NC-NC | 266635_at | At2g35470 | expressed protein (68415.m04345) |
| 219 | Not regulated | NC-NC-NC | 266639_at | At2g35520 | defender against cell death 2 (DAD2) identical to defender against cell death 2 (DAD-2, AtDAD2) [Arabidopsis thaliana] SWISS- |
| PROT: O22622 (68415.m04350) | |||||
| 220 | Not regulated | NC-NC-NC | 266641_at | At2g35605 | SWIB complex BAF60b domain-containing protein contains Pfam profile PF02201: BAF60b domain of the SWIB complex |
| (68415.m04363) | |||||
| 221 | Not regulated | NC-NC-NC | 266642_at | At2g35410 | 33 kDa ribonucleoprotein, chloroplast, putative/RNA-binding protein cp33, putative similar to SP|P19684 33 kDa |
| ribonucleoprotein, chloroplast precursor {Nicotiana sylvestris}; contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA | |||||
| recognition motif) (RRM) (68415.m04340) | |||||
| 222 | Not regulated | NC-NC-NC | 266608_at | At2g35500 | shikimate kinase-related low similarity to shikimate kinase precursor from Lycopersicon esculentum [SP|Q00497] (68415.m04348) |
| 223 | Not regulated | NC-NC-NC | 266613_at | At2g14900 | gibberellin-regulated family protein similar to SP|P46690 Gibberellin-regulated protein 4 precursor {Arabidopsis thaliana} GASA4; |
| contains Pfam profile PF02704: Gibberellin regulated protein (68415.m01694) | |||||
| 224 | Not regulated | NC-NC-NC | 266587_at | At2g14880 | SWIB complex BAF60b domain-containing protein contains Pfam profile PF02201: BAF60b domain of the SWIB complex |
| (68415.m01691) | |||||
| 225 | Not regulated | NC-NC-NC | 266605_at | At2g46020 | transcription regulatory protein SNF2, putative similar to SP|P22082 Transcription regulatory protein SNF2 (SWI/SNF complex |
| component SNF2) {Saccharomyces cerevisiae}; contains Pfam profiles PF00271: Helicase conserved C-terminal domain, | |||||
| PF00176: SNF2 family N-terminal domain (68415.m05724) | |||||
| 226 | Not regulated | NC-NC-NC | 266581_at | At2g46140 | late embryogenesis abundant protein, putative/LEA protein, putative similar to SP|P46518 Late embryogenesis abundant protein |
| Lea14-A {Gossypium hirsutum}; contains Pfam profile PF03168: Late embryogenesis abundant protein (68415.m05738) | |||||
| 227 | Not regulated | NC-NC-NC | 266582_at | At2g46090 | diacylglycerol kinase family protein contains INTERPRO domain, IPR001206, DAG-kinase catalytic domain (68415.m05733) |
| 228 | Not regulated | NC-NC-NC | 266553_at | At2g46170 | reticulon family protein (RTNLB5) weak similarity to Nogo-C protein [Rattus norvegicus] GI: 6822251; contains Pfam profile |
| PF02453: Reticulon (68415.m05741) | |||||
| 229 | Not regulated | NC-NC-NC | 266554_s_at | At2g46280 | eukaryotic translation initiation factor 3 subunit 2/TGF-beta receptor interacting protein 1/eIF-3 beta/eIF3i/TRIP-1 (TIF3I1) |
| identical to eukaryotic translation initiation factor 3 subunit 2 (SP: Q38684) {Arabidopsis thaliana}; contains Pfam PF00400: WD | |||||
| domain, G-beta repeat (5 copies) (68415.m05755) | |||||
| 230 | Not regulated | NC-NC-NC | 266557_at | At2g46080 | expressed protein (68415.m05732) |
| 231 | Not regulated | NC-NC-NC | 266566_at | At2g24040 | hydrophobic protein, putative/low temperature and salt responsive protein, putative similar to SP|Q9ZNQ7 Hydrophobic protein |
| RCI2A (Low temperature and salt responsive protein LTI6A) {Arabidopsis thaliana}; contains Pfam profile PF01679: | |||||
| Uncharacterized protein family (68415.m02872) | |||||
| 232 | Not regulated | NC-NC-NC | 266572_at | At2g23840 | HNH endonuclease domain-containing protein contains Pfam profile PF01844: HNH endonuclease (68415.m02848) |
| 233 | Not regulated | NC-NC-NC | 266575_at | At2g24060 | translation initiation factor 3 (IF-3) family protein similar to SP|P33319 Translation initiation factor IF-3 {Proteus vulgaris}; contains |
| Pfam profiles PF00707: Translation initiation factor IF-3, C-terminal domain, PF05198: Translation initiation factor IF-3, N-terminal | |||||
| domain (68415.m02874) | |||||
| 234 | Not regulated | NC-NC-NC | 266576_at | At2g23940 | expressed protein contains 2 transmembrane domains; contains Pfam profile PF05620: Protein of unknown function (DUF788) |
| (68415.m02858) | |||||
| 235 | Not regulated | NC-NC-NC | 266520_at | At2g23980 | cyclic nucleotide-regulated ion channel/cyclic nucleotide-gated channel (CNGC6) identical to cyclic nucleotide and calmodulin- |
| regulated ion channel (cngc6) GI: 4581207 from [Arabidopsis thaliana] (68415.m02863) | |||||
| 236 | Not regulated | NC-NC-NC | 266521_at | At2g24020 | expressed protein contains Pfam domain PF02575: Uncharacterized BCR, YbaB family COG0718 (68415.m02869) |
| 237 | Not regulated | NC-NC-NC | 266534_at | At2g16940 | RNA recognition motif (RRM)-containing protein (68415.m01952) |
| 238 | Not regulated | NC-NC-NC | 266536_at | At2g16900 | expressed protein (68415.m01946) |
| 239 | Not regulated | NC-NC-NC | 266537_at | At2g16860 | GCIP-interacting family protein similar to GCIP-interacting protein mp29 (GI: 27372623) [Mus musculus]; similar to GCIP-interacting |
| protein P29 (GI: 11967379) [Homo sapiens] (68415.m01939) | |||||
| 240 | Not regulated | NC-NC-NC | 266541_at | At2g35110 | HEM protein-related weak similarity to Membrane-associated protein Hem (Dhem-2) (Swiss-Prot: P55162) [Drosophila |
| melanogaster]; weak similarity to Nck-associated protein 1 (NAP 1) (p125Nap1) (Membrane-associated protein HEM-2) (Swiss- | |||||
| Prot: P55161)[Rattus norvegicus] (68415.m04307) | |||||
| 241 | Not regulated | NC-NC-NC | 266546_at | At2g35270 | DNA-binding protein-related contains Pfam domain PF03479: Domain of unknown function (DUF296), found in AT-hook motifs |
| Pfam: PF02178 (68415.m04326) | |||||
| 242 | Not regulated | NC-NC-NC | 266547_at | At2g35320 | expressed protein (68415.m04331) |
| 243 | Not regulated | NC-NC-NC | 266551_at | At2g35260 | expressed protein (68415.m04325) |
| 244 | Not regulated | NC-NC-NC | 266517_at | At2g35120 | glycine cleavage system H protein, mitochondrial, putative similar to SP|Q39732 Glycine cleavage system H protein, mitochondrial |
| precursor {Flaveria anomala}; contains Pfam profile PF01597: Glycine cleavage H-protein (68415.m04308) | |||||
| 245 | Not regulated | NC-NC-NC | 266519_at | At2g35155 | expressed protein (68415.m04312) |
| 246 | Not regulated | NC-NC-NC | 266509_at | At2g47940 | DegP2 protease (DEGP2) identical to DegP2 protease GI: 13172275 from [Arabidopsis thaliana]; identical to cDNA DegP2 protease |
| (DEGP2) nuclear gene for chloroplast product GI: 13172274 (68415.m05995) | |||||
| 247 | Not regulated | NC-NC-NC | 266510_at | At2g47990 | transducin family protein/WD-40 repeat family protein similar to Vegetatible incompatibility protein HET-E-1 (SP: Q00808) |
| {Podospora anserina}; contains 5 WD-40 repeats (PF00400); similar to beta transducin-like protein HET-E2C*4 | |||||
| (GP: 17225206)[Podospora anserina] (68415.m06006) | |||||
| 248 | Not regulated | NC-NC-NC | 266512_at | At2g47690 | NADH-ubiquinone oxidoreductase-related contains weak similarity to NADH-ubiquinone oxidoreductase 15 kDa subunit (EC |
| 1.6.5.3) (EC 1.6.99.3) (Complex I-15 kDa) (CI-15 kDa) (Swiss-Prot: O43920) [Homo sapiens (68415.m05956) | |||||
| 249 | Not regulated | NC-NC-NC | 266513_at | At2g47700 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type(RING finger) |
| (68415.m05957) | |||||
| 250 | Not regulated | NC-NC-NC | 266514_at | At2g47890 | zinc finger (B-box type) family protein (68415.m05981) |
| 251 | Not regulated | NC-NC-NC | 266482_at | At2g47640 | small nuclear ribonucleoprotein D2, putative/snRNP core protein D2, putative/Sm protein D2, putative similar to small nuclear |
| ribonucleoprotein Sm D2 (snRNP core protein D2) (Sm-D2) [Mus musculus] SWISS-PROT: P43330 (68415.m05944) | |||||
| 252 | Not regulated | NC-NC-NC | 266483_at | At2g47910 | expressed protein (68415.m05987) |
| 253 | Not regulated | NC-NC-NC | 266485_at | At2g47630 | esterase/lipase/thioesterase family protein similar to monoglyceride lipase from [Homo sapiens] GI: 14594904, [Mus musculus] |
| GI: 2632162; contains Interpro entry IPR000379 (68415.m05942) | |||||
| 254 | Not regulated | NC-NC-NC | 266459_at | At2g47970 | NPL4 family protein contains Pfam domain, PF05021: NPL4 family (68415.m06001) |
| 255 | Not regulated | NC-NC-NC | 266460_at | At2g47930 | hydroxyproline-rich glycoprotein family protein contains proline-rich extensin domains, INTERPRO: IPR002965 (68415.m05993) |
| 256 | Not regulated | NC-NC-NC | 266468_at | At2g47960 | expressed protein (68415.m05999) |
| 257 | Not regulated | NC-NC-NC | 266474_at | At2g31110 | expressed protein (68415.m03799) |
| 258 | Not regulated | NC-NC-NC | 266476_at | At2g31090 | expressed protein (68415.m03797) |
| 259 | Not regulated | NC-NC-NC | 266478_at | At2g31170 | tRNA synthetase class I (C) family protein similar to cysteine-tRNA ligase [Escherichia coli] GI: 41203: contains Pfam profile |
| PF01406: tRNA synthetases class I (C) (68415.m03805) | |||||
| 260 | Not regulated | NC-NC-NC | 266481_at | At2g31070 | TCP family transcription factor, putative similar to TCP1 protein (GI: 20269127) {Lupinus albus} and cycloidea (GI: 12002867) |
| [Lycopersicon esculentum] (68415.m03791) | |||||
| 261 | Not regulated | NC-NC-NC | 266445_at | At2g43105 | |
| 262 | Not regulated | NC-NC-NC | 266449_at | At2g43080 | oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to prolyl 4-hydroxylase, alpha subunit, from Homo sapiens |
| [GI: 18073925]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain (68415.m05346) | |||||
| 263 | Not regulated | NC-NC-NC | 266451_at | At2g43090 | aconitase C-terminal domain-containing protein contains Pfam profile PF00694: Aconitase C-terminal domain (68415.m05348) |
| 264 | Not regulated | NC-NC-NC | 266394_at | At2g43130 | Ras-related protein (ARA-4)/small GTP-binding protein, putative identical to SP: P28187 Ras-related protein ARA-4 {Arabidopsis |
| thaliana} (68415.m05356) | |||||
| 265 | Not regulated | NC-NC-NC | 266407_at | At2g38560 | transcription factor S-II (TFIIS) domain-containing protein similar to SP|P49373 Transcription elongation factor S-II (TFIIS) |
| {Schizosaccharomyces pombe}; contains Pfam profile PF01096: Transcription factor S-II (TFIIS) (68415.m04737) | |||||
| 266 | Not regulated | NC-NC-NC | 266410_at | At2g38770 | expressed protein (68415.m04760) |
| 267 | Not regulated | NC-NC-NC | 266413_at | At2g38740 | haloacid dehalogenase-like hydrolase family protein similar to SP|P71447 Beta-phosphoglucomutase (EC 5.4.2.6) {Lactococcus |
| lactis}; contains InterPro accession IPR005834: Haloacid dehalogenase-like hydrolase (68415.m04757) | |||||
| 268 | Not regulated | NC-NC-NC | 266417_at | At2g38550 | expressed protein contains Pfam profile PF03647: Uncharacterised protein family (UPF0136) (68415.m04736) |
| 269 | Not regulated | NC-NC-NC | 266418_at | At2g38750 | annexin 4 (ANN4) nearly identical to annexin (AnnAt4) [Arabidopsis thaliana] GI: 6503084; contains Pfam profile PF00191: Annexin |
| (68415.m04758) | |||||
| 270 | Not regulated | NC-NC-NC | 266421_at | At2g38540 | nonspecific lipid transfer protein 1 (LTP1) identical to SP|Q42589 (68415.m04735) |
| 271 | Not regulated | NC-NC-NC | 266363_at | At2g41250 | haloacid dehalogenase-like hydrolase family protein low similarity to SP|Q94915 Rhythmically expressed gene 2 protein (DREG-2) |
| [Drosophila melanogaster); contains InterPro accession IPR005834: Haloacid dehalogenase-like hydrolase (68415.m05094) | |||||
| 272 | Not regulated | NC-NC-NC | 266370_at | At2g41350 | expressed protein (68415.m05104) |
| 273 | Not regulated | NC-NC-NC | 266356_at | At2g32300 | uclacyanin I identical to uclacyanin I GI: 3399767 from [Arabidopsis thaliana]; contains Pfam profile PF02298: Plastocyanin-like |
| domain; identical to cDNA uclacyanin I GI: 3399766 (68415.m03949) | |||||
| 274 | Not regulated | NC-NC-NC | 266359_at | At2g32260 | cholinephosphate cytidylyltransferase, putative/phosphorylcholine transferase, putative/CTP: phosphocholine cytidylyltransferase, |
| putative strong similarity to CTP: phosphocholine cytidylyltransferase [Brassica napus] GI: 1418125; contains Pfam profile PF01467: | |||||
| Cytidylyltransferase (68415.m03943) | |||||
| 275 | Not regulated | NC-NC-NC | 266334_at | At2g32380 | expressed protein (68415.m03957) |
| 276 | Not regulated | NC-NC-NC | 266342_at | At2g01540 | C2 domain-containing protein similar to zinc finger and C2 domain protein GI: 9957238 from [Arabidopsis thaliana] (68415.m00078) |
| 277 | Not regulated | NC-NC-NC | 266345_at | At2g01410 | hypothetical protein (68415.m00060) |
| 278 | Not regulated | NC-NC-NC | 266328_at | At2g01600 | epsin N-terminal homology (ENTH) domain-containing protein contains Pfam PF01417: ENTH domain. ENTH (Epsin N-terminal |
| homology) domain; similar to clathrin assembly protein AP180 (GI: 6492344) [Xenopus laevis] (68415.m00084) | |||||
| 279 | Not regulated | NC-NC-NC | 266329_at | At2g01590 | expressed protein (68415.m00083) |
| 280 | Not regulated | NC-NC-NC | 266331_at | At2g01570 | gibberellin response modulator (RGA1)/gibberellin-responsive modulator identical to GB: Y11338, member of SCARECROW |
| family (68415.m00081) | |||||
| 281 | Not regulated | NC-NC-NC | 266300_at | At2g01420 | auxin transport protein, putative similar to auxin transport protein PIN7[Arabidopsis thaliana] gi|5817305|gb|AAD52697 |
| (68415.m00062) | |||||
| 282 | Not regulated | NC-NC-NC | 266303_at | At2g27060 | leucine-rich repeat transmembrane protein kinase, putative (68415.m03251) |
| 283 | Not regulated | NC-NC-NC | 266306_at | At2g26970 | exonuclease family protein contains exonuclease domain, Pfam: PF00929 (68415.m03235) |
| 284 | Not regulated | NC-NC-NC | 266310_at | At2g26990 | COP9 signalosome complex subunit 2/CSN complex subunit 2 (CSN2) proteasome, COP9-complex and elF3-domain protein: |
| identical to CSN complex subunit 2 [Arabidopsis thaliana] GI: 18056655; identical to cDNA CSN complex subunit 2 (CSN2) | |||||
| GI: 18056654 (68415.m03241) | |||||
| 285 | Not regulated | NC-NC-NC | 266314_at | At2g27040 | PAZ domain-containing protein/piwi domain-containing protein similar to SP|Q9QZ81 Eukaryotic translation initiation factor 2C 2 |
| {Rattus norvegicus}; contains Pfam profiles PF02171: Piwi domain, PF02170: PAZ domain (68415.m03249) | |||||
| 286 | Not regulated | NC-NC-NC | 266315_at | At2g27100 | C2H2 zinc-finger protein SERRATE (SE) identical to C2H2 zinc-finger protein SERRATE GI: 14488602 from [Arabidopsis thaliana] |
| (68415.m03256) | |||||
| 287 | Not regulated | NC-NC-NC | 266317_at | At2g27030 | calmodulin-2/3/5 (CAM5) (TCH1) identical to calmodulin GI: 474183 from [Arabidopsis thaliana]. SP|P25069 Calmodulin-2/3/5 |
| {Arabidopsis thaliana} (68415.m03245) | |||||
| 288 | Not regulated | NC-NC-NC | 266319_s_at | At2g46720 | fatty acid elongase 3-ketoacyl-CoA synthase, putative similar to GI: 4091810; contains Pfam profile PF02797: Chalcone and |
| stilbene synthases, C-terminal domain (68415.m05829) | |||||
| 289 | Not regulated | NC-NC-NC | 266322_at | At2g46690 | auxin-responsive family protein similar to indole-3-acetic acid induced protein ARG7 (SP: P32295) [Phaseolus aureus] |
| (68415.m05826) | |||||
| 290 | Not regulated | NC-NC-NC | 266271_at | At2g29440 | glutathione S-transferase, putative (68415.m03577) |
| 291 | Not regulated | NC-NC-NC | 266275_at | At2g29370 | tropinone reductase, putative/tropine dehydrogenase, putative similar to SP|P50162 Tropinone reductase-I (EC 1.1.1.206) (TR-I) |
| (Tropine dehydrogenase) {Datura stramonium} (68415.m03568) | |||||
| 292 | Not regulated | NC-NC-NC | 266277_at | At2g29310 | tropinone reductase, putative/tropine dehydrogenase, putative similar to tropinone reductase SP: P50165 from [Datura |
| stramonium] (68415.m03560) | |||||
| 293 | Not regulated | NC-NC-NC | 266279_at | At2g29290 | tropinone reductase, putative/tropine dehydrogenase, putative similar to tropinone reductase SP: P50165 from [Datura |
| stramonium] (68415.m03558) | |||||
| 294 | Not regulated | NC-NC-NC | 266285_at | At2g29180 | expressed protein (68415.m03547) |
| 295 | Not regulated | NC-NC-NC | 266289_at | At2g29390 | sterol 4-alpha-methyl-oxidase 1 (SMO1) nearly identical to sterol 4-alpha-methyl-oxidase GI: 18973469 from [Arabidopsis thaliana]; |
| identical to cDNA sterol 4-alpha-methyl-oxidase (SMO) partial cds, GI: 16973431 (68415.m03570) | |||||
| 296 | Not regulated | NC-NC-NC | 266291_at | At2g29320 | tropinone reductase, putative/tropine dehydrogenase, putative similar to tropinone reductase SP: P50165 from [Datura |
| stramonium] (68415.m03561) | |||||
| 297 | Not regulated | NC-NC-NC | 266293_at | At2g29360 | tropinone reductase, putative/tropine dehydrogenase, putative similar to tropinone reductase SP: P50165 from [Datura |
| stramonium] (68415.m03567) | |||||
| 298 | Not regulated | NC-NC-NC | 266237_at | At2g29540 | DNA-directed RNA polymerase I(A) and III(C) 14 kDa subunit (RPAC14) identical to RNA polymerase I(A) and III(C) 14 kDa subunit |
| (AtRPAC14) [Arabidopsis thaliana] GI: 1184686 (68415.m03588) | |||||
| 299 | Not regulated | NC-NC-NC | 266238_at | At2g29400 | serine/threonine protein phosphatase PP1 isozyme 1 (TOPP1)/phosphoprotein phosphatase 1 identical to SP|P30366| |
| Serine/threonine protein phosphatase PP1 isozyme 1 (EC 3.1.3.16) {Arabidopsis thaliana}, phosphoprotein phosphatase 1 | |||||
| GI: 166572 [Arabidopsis thaliana] (68415.m03573) | |||||
| 300 | Not regulated | NC-NC-NC | 266239_at | At2g29530 | mitochondrial import inner membrane translocase (TIM10) identical to mitochondrial import inner membrane translocase subunit |
| Tim10 [Arabidopsis thaliana] Swiss-Prot: Q9ZW33; contains Pfam domain, PF02953: Tim10/DDP family zinc finger (68415.m03587) | |||||
| 301 | Not regulated | NC-NC-NC | 266247_at | At2g27660 | DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain (68415.m03352) |
| 302 | Not regulated | NC-NC-NC | 266253_at | At2g27840 | histone deacetylase-related/HD-related similar to nucleolar histone deacetylase HD2-p39 [Zea mays] GI: 2257756; contains non- |
| consensus donor splice site AT at exon2 and acceptor splice site AC at exon3. (68415.m03375) | |||||
| 303 | Not regulated | NC-NC-NC | 266257_at | At2g27820 | prephenate dehydratase family protein (68415.m03373) |
| 304 | Not regulated | NC-NC-NC | 266260_at | At2g27600 | AAA-type ATPase family protein/vacuolar sorting protein-related similar to SP|P46467 SKD1 protein (Vacuolar sorting protein 4b) |
| {Mus musculus}; contains Pfam profiles PF00004: ATPase AAA family, PF04212: MIT domain (68415.m03346) | |||||
| 305 | Not regulated | NC-NC-NC | 266261_at | At2g27580 | zinc finger (AN1-like) family protein contains Pfam domains, PF01428: AN1-like Zinc finger and PF01754: A20-like zinc finger |
| (68415.m03342) | |||||
| 306 | Not regulated | NC-NC-NC | 266206_at | At2g27730 | expressed protein contains 1 transmembrane domain; similar to Unknown mitochondrial protein At2g27730 (Swiss-Prot: Q9ZUX4) |
| [Arabidopsis thaliana] similar to F1F0-ATPase inhibitor protein (GI: 5821432) [Oryza sativa) (68415.m03361) | |||||
| 307 | Not regulated | NC-NC-NC | 266216_at | At2g28810 | Dof-type zinc finger domain-containing protein similar to zinc finger protein OBP2 GI: 5059394 from [Arabidopsis thaliana] |
| (68415.m03503) | |||||
| 308 | Not regulated | NC-NC-NC | 266221_at | At2g28780 | NAD-dependent epimerase/dehydratase family protein similar to UDP-glucuronic acid decarboxylase Uxs1p from Filobasidiella |
| neoformans GI: 14318327: contains Pfam profile PF01370 NAD dependent epimerase/dehydratase family (68415.m03497) | |||||
| 309 | Not regulated | NC-NC-NC | 266223_at | At2g28790 | osmotin-like protein, putative similar to SP|Q41350 Osmotin-like protein precursor {Lycopersicon esculentum}; contains Pfam profile |
| PF00314: Thaumatin family (68415.m03500) | |||||
| 310 | Not regulated | NC-NC-NC | 266224_at | At2g28800 | chloroplast membrane protein (ALBINO3) Oxa1p homolog {PMID: 11148275}; identical to chloroplast membrane protein ALBINO3 |
| [Arabidopsis thaliana] GI: 2209332 (68415.m03501) | |||||
| 311 | Not regulated | NC-NC-NC | 266228_at | At2g28910 | CAX-interacting protein 4 (CAXIP4) contains Pfam domain PF00098: Zinc knuckle; identical to cDNA CAX-interacting protein 4 |
| GI: 27651998 (68415.m03513) | |||||
| 312 | Not regulated | NC-NC-NC | 266229_at | At2g28840 | ankyrin repeat family protein contains ankyrin repeats, Pfam: PF00023 (68415.m03506) |
| 313 | Not regulated | NC-NC-NC | 266202_at | At2g02400 | cinnamoyl-CoA reductase family similar to cinnamoyl-CoA reductase from Pinus taeda [GI: 17978649], Eucalyptus gunnii |
| [GI: 2058311] (68415.m00180) | |||||
| 314 | Not regulated | NC-NC-NC | 266180_at | At2g02470 | PHD finger family protein contains Pfam domain, PF00628: PHD-finger (68415.m00186) |
| 315 | Not regulated | NC-NC-NC | 266181_at | At2g02390 | glutathione S-transferase zeta 1 (GSTZ1) (GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione S-transferase zeta-class 1 |
| (EC 2.5.1.18) (AIGSTZ1) (Maleylacetone isomerase) (EC 5.2.1.—) (MAI) {Arabidopsis thallium}; contains Pfam profiles PF02798: | |||||
| Glutathione S-transferase, N-terminal domain and PF00043: Glutathione S-transferase, C-terminal domain (68415.m00177) | |||||
| 316 | Not regulated | NC-NC-NC | 266188_at | At2g39000 | GCN5-related N-acetyltransferase (GNAT) family protein contains Pfam profile PF00583: acetyltransferase, GNAT family |
| (68415.m04793) | |||||
| 317 | Not regulated | NC-NC-NC | 266189_at | At2g39020 | GCN5-related N-acetyltransferase (GNAT) family protein similar to SP|Q9SMB8 Tyramine N-feruloyltransferase 4/11 (EC 2.3.1.110) |
| (Hydroxycinnamoyl-CoA: tyramine N-hydroxycinnamoyltransferase) {Nicotiana tabacum}; contains Pfam profile PF00583: | |||||
| acetyltransferase, GNAT family (68415.m04797) | |||||
| 318 | Not regulated | NC-NC-NC | 266192_at | At2g39070 | |
| 319 | Not regulated | NC-NC-NC | 266193_at | At2g39080 | expressed protein (68415.m04802) |
| 320 | Not regulated | NC-NC-NC | 266168_at | At2g38870 | protease inhibitor, putative similar to SP|P24076 Glu S. griseus protease inhibitor (BGIA) {Momordica charantia}; contains Pfam |
| profile PF00280: Potato inhibitor I family (68415.m04776) | |||||
| 321 | Not regulated | NC-NC-NC | 266158_at | At2g28070 | ABC transporter family protein (68415.m03408) |
| 322 | Not regulated | NC-NC-NC | 266165_at | At2g28190 | superoxide dismutase [Cu—Zn], chloroplast (SODCP)/copper/zinc superoxide dismutase (CSD2) identical to |
| GP: 3273753: AF061519 (68415.m03423) | |||||
| 323 | Not regulated | NC-NC-NC | 266138_at | At2g28060 | protein kinase-related similar to GAL83 protein [Solanum tuberosum] GI: 5702015; contains Pfam profile PF04739: 5′-AMP- |
| activated protein kinase, beta subunit, complex-interacting region; supporting cDNA gi|22652783|gb|AF491295.1| (68415.m03406) | |||||
| 324 | Not regulated | NC-NC-NC | 266117_at | At2g02170 | remorin family protein contains Pfam domain, PF03763: Remorin, C-terminal region (68415.m00153) |
| 325 | Not regulated | NC-NC-NC | 266118_at | At2g02130 | plant defensin-fusion protein, putative (PDF2.3) plant defensin protein family member, personal communication, Bart Thomma |
| (Bart. Thomma@agr.kuleuven.ac.be) (68415.m00149) | |||||
| 326 | Not regulated | NC-NC-NC | 266119_at | At2g02100 | plant defensin-fusion protein, putative (PDF2.2) plant defensin protein family member, personal communication, Bart Thomma |
| (Bart.Thomma@agr.kuleuven.ac.be); similar to SWISS-PROT: O65740 (68415.m00146) | |||||
| 327 | Not regulated | NC-NC-NC | 266120_at | At2g02070 | zinc finger (C2H2 type) family protein contains Pfam domain, PF00096: Zinc finger, C2H2 type (68415.m00143) |
| 328 | Not regulated | NC-NC-NC | 266121_at | At2g02160 | zinc finger (CCCH-type) family protein contains Pfam domain, PF00642: Zinc forger C-x8-C-x5-C-x3-H type (and similar) |
| (68415.m00152) | |||||
| 329 | Not regulated | NC-NC-NC | 266123_at | At2g45180 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein similar to 14 kDa polypeptide [Catharanthus roseus] |
| GI: 407410; contains Pfam protease inhibitor/seed storage/LTP family domain PF00234 (68415.m05625) | |||||
| 330 | Not regulated | NC-NC-NC | 266137_at | At2g45010 | expressed protein weak similarity to PGPS/D12 [Petunia x hybrida] GI: 4105794; contains Pfam profile PF04749: Protein of |
| unknown function, DUF614 (68415.m05604) | |||||
| 331 | Not regulated | NC-NC-NC | 266103_at | At2g45140 | vesicle-associated membrane protein, putative/VAMP, putative similar to VAP27 GI: 6688926 [Nicotiana plumbaginifolia] |
| (68415.m05616) | |||||
| 332 | Not regulated | NC-NC-NC | 266079_at | At2g37860 | expressed protein (68415.m04647) |
| 333 | Not regulated | NC-NC-NC | 266090_at | At2g38000 | chaperone protein dnaJ-related weak similarity to Chaperone protein dnaJ (Swiss-Prot: Q9ZFC5) [Methylovorus sp.] |
| (68415.m04664) | |||||
| 334 | Not regulated | NC-NC-NC | 266094_at | At2g37975 | expressed protein (68415.m04661) |
| 335 | Not regulated | NC-NC-NC | 266095_at | At2g38050 | 3-oxo-5-alpha-steroid 4-dehydrogenase, putative/steroid 5-alpha-reductase, putative identical to gi: 1280611; contains a steroid 5- |
| alpha reductase, C-terminal domain (68415.m04671) | |||||
| 336 | Not regulated | NC-NC-NC | 266096_at | At2g38020 | vacuoleless1 (VCL1) contains Pfam profiles: PF04841 Vps16, N-terminal region, PF04840: Vps16, C-terminal region; identical to |
| cDNA VCL1 (VCL1) GI: 13877132 (68415.m04667) | |||||
| 337 | Not regulated | NC-NC-NC | 266101_at | At2g37940 | expressed protein (68415.m04657) |
| 338 | Not regulated | NC-NC-NC | 266057_at | At2g40660 | tRNA-binding region domain-containing protein similar to SP|Q12904 Multisynthetase complex auxiliary component p43 [Contains; |
| Endothelial-monocyte activating polypeptide li (EMAP-II) (Small inducible cytokine subfamily E member 1)] {Homo sapiens}; | |||||
| contains Pfam profile PF01588: Putative tRNA binding domain (68415.m05017) | |||||
| 339 | Not regulated | NC-NC-NC | 266059_at | At2g40765 | expressed protein (68415.m05029) |
| 340 | Not regulated | NC-NC-NC | 266062_at | At2g18730 | diacylglycerol kinase, putative contains INTERPRO domain, IPR001206, DAG-kinase catalytic domain (68415.m02181) |
| 341 | Not regulated | NC-NC-NC | 266073_at | At2g18770 | expressed protein (68415.m02185) |
| 342 | Not regulated | NC-NC-NC | 266074_at | At2g18740 | small nuclear ribonucleoprotein E, putative/snRNP-E, putative/Sm protein E, putative similar to SWISS-PROT: P08578 small |
| nuclear ribonucleoprotein E (snRNP-E) (Sm protein E, Sm-E, SmE) [Chicken] (68415.m02182) | |||||
| 343 | Not regulated | NC-NC-NC | 266017_at | At2g18690 | expressed protein (68415.m02177) |
| 344 | Not regulated | NC-NC-NC | 266018_at | At2g18710 | preprotein translocase secY subunit, chloroplast (CpSecY) Identical to SP|Q38885 Preprotein translocase secY subunit, chloroplast |
| precursor (CpSecY) {Arabidopsis thaliana} (68415.m02179) | |||||
| 345 | Not regulated | NC-NC-NC | 266035_at | At2g05990 | enoyl-[acyl-carrier protein] reductase [NADH], chloroplast, putative/NADH-dependent enoyl-ACP reductase, putative strong |
| similarity to enoyl-[acyl-carrier protein] reductase [NADH] SP: P80030 from [Brassica napus] (68415.m00651) | |||||
| 346 | Not regulated | NC-NC-NC | 266038_s_at | At2g05840 | 20S proteasome alpha subunit A2 (PAA2) identical to GB: AF043519 (68415.m00632) |
| 347 | Not regulated | NC-NC-NC | 266037_at | At2g05940 | protein kinase, putative similar to auxin-regulated dual specificity cytosolic kinase [Lycopersicon esculentum] |
| gi|14484938|gb|AAK62821 (68415.m00645) | |||||
| 348 | Not regulated | NC-NC-NC | 266015_at | At2g24190 | short-chain dehydrogenase/reductase (SDR) family protein contains INTERPRO family IPR002198 short-chain |
| dehydrogenase/reductase (SDR) superfamily (68415.m02890) | |||||
| 349 | Not regulated | NC-NC-NC | 265990_at | At2g24280 | serine carboxypeptidase S2B family protein contains Pfam profile: PF05577 Serine carboxypeptidase S2B (68415.m02901) |
| 350 | Not regulated | NC-NC-NC | 265998_at | At2g24270 | NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, putative similar to NADP-dependent glyceraldehyde-3-phosphate |
| dehydrogenase (NON-phosphorylating glyceraldehyde 3-phosphate; glyceraldehyde-3-phosphate dehydrogenase [NADP+]) | |||||
| [Nicotiana plumbaginifolia] SWISS-PROT: P93338 (68415.m02899) | |||||
| 351 | Not regulated | NC-NC-NC | 265999_at | At2g24100 | expressed protein (68415.m02879) |
| 352 | Not regulated | NC-NC-NC | 266000_at | At2g24180 | cytochrome P450 family protein (68415.m02889) |
| 353 | Not regulated | NC-NC-NC | 266001_at | At2g24150 | expressed protein contains Pfam profile PF03006: Uncharacterised protein family (Hly-III/UPF0073) (68415.m02885) |
| 354 | Not regulated | NC-NC-NC | 265953_at | At2g37490 | |
| 355 | Not regulated | NC-NC-NC | 265958_at | At2g37250 | adenylate kinase family protein contains Pfam profile: PF00406 adenylate kinase (68415.m04570) |
| 356 | Not regulated | NC-NC-NC | 265959_at | At2g37240 | expressed protein weak similarity to fmHP (GI: 22902096) [Takifugu rubripes] (68415.m04569) |
| 357 | Not regulated | NC-NC-NC | 265960_at | At2g37470 | histone H2B, putative strong similarity to histone H2B from Lycopersicon esculentum GI: 3021483, GI: 3021485, Capsicum annuum |
| SP|O49118; contains Pfam profile PF00125 Core histone H2A/H2B/H3/H4 (68415.m04596) | |||||
| 358 | Not regulated | NC-NC-NC | 265962_at | At2g37460 | nodulin MtN21 family protein similar to MtN21 GI: 2598575 (root nodule development) from [Medicago truncatula] (68415.m04595) |
| 359 | Not regulated | NC-NC-NC | 265963_s_at | At2g37270 | 40S ribosomal protein S5 (RPS5A) identical to GP: 3043428 (68415.m04572) |
| 360 | Not regulated | NC-NC-NC | 265965_at | At2g37500 | arginine biosynthesis protein ArgJ family contains Pfam profile: PF01960 ArgJ family (68415.m04599) |
| 361 | Not regulated | NC-NC-NC | 265966_at | At2g37220 | 29 kDa ribonucleoprotein, chloroplast, putative/RNA-binding protein cp29, putative similar to SP|Q43349 29 kDa |
| ribonucleoprotein, chloroplast precursor (RNA-binding protein cp29) {Arabidopsis thaliana} (68415.m04566) | |||||
| 362 | Not regulated | NC-NC-NC | 265968_at | At2g37410 | mitochondrial import inner membrane translocase (TIM17) nearly identical to SP|Q9SP35 Mitochondrial import inner membrane |
| translocase subunit TIM17 {Arabidopsis thaliana} (68415.m04587) | |||||
| 363 | Not regulated | NC-NC-NC | 265930_at | At2g18510 | pre-mRNA splicing factor, putative similar to SP|Q15427 Splicing factor 3B subunit 4 (Spliceosome associated protein 49) (SAP 49) |
| (SF3b50) (Pre-mRNA splicing factor SF3b 49 kDa subunit) {Homo sapiens}; contains Pfam profile PF00076: RNA recognition motif. | |||||
| (a.k.a. RRM, RBD, or RNP domain) (68415.m02157) | |||||
| 364 | Not regulated | NC-NC-NC | 265940_at | At2g19480 | nucleosome assembly protein (NAP), putative similar to nucleosome assembly protein 1 [Glycine max] GI: 1161252; contains Pfam |
| profile PF00956: Nudeosome assembly protein (NAP) (68415.m02277) | |||||
| 365 | Not regulated | NC-NC-NC | 265943_at | At2g19570 | cytidine deaminase (CDD)/cytidine aminohydrolase identical to cytidine deaminase (CDD) [Arabidopsis thaliana] GI: 3046700 |
| (68415.m02286) | |||||
| 366 | Not regulated | NC-NC-NC | 265947_at | At2g19540 | transducin family protein/WD-40 repeat family protein contains WD-40 repeats (PF00400); similar to Glutamate-rich WD repeat |
| protein (GRWD) (SP: Q9BQ67)[Homo sapiens] (68415.m02283) | |||||
| 367 | Not regulated | NC-NC-NC | 265918_at | At2g15090 | fatty acid elongase, putative similar to fatty acid elongase 1 [GI: 881615] (68415.m01720) |
| 368 | Not regulated | NC-NC-NC | 265920_s_at | At2g15120 | |
| 369 | Not regulated | NC-NC-NC | 265894_at | At2g15050 | lipid transfer protein, putative similar to SP|Q42641 nonspecific lipid-transfer protein A precursor (LTP A) (Wax-associated protein |
| 9A) {Brassica oleracea}; contains Pfam Protease inhibitor/seed storage/LTP family domain PF00234 (68415.m01714) | |||||
| 370 | Not regulated | NC-NC-NC | 265895_at | At2g15000 | expressed protein (68415.m01707) |
| 371 | Not regulated | NC-NC-NC | 265909_at | At2g25720 | expressed protein (68415.m03083) |
| 372 | Not regulated | NC-NC-NC | 265911_at | At2g25670 | expressed protein (68415.m03076) |
| 373 | Not regulated | NC-NC-NC | 265887_at | At2g25710 | holocarboxylase synthetase 1 (HCS1) identical to holocarboxylase synthetase 1 (hcs1) [Arabidopsis thaliana] GI: 19698365 |
| (68415.m03081) | |||||
| 374 | Not regulated | NC-NC-NC | 265858_at | At2g01720 | ribophorin I family protein similar to SP|P04843 Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit |
| precursor (EC 2.41.119) (Ribophorin I) {Homo sapiens}; contains Pfam profile PF04597: Ribophorin I (68415.m00100) | |||||
| 375 | Not regulated | NC-NC-NC | 265866_at | At2g01710 | DNAJ heat shock N-terminal domain-containing protein simlar to AHM1 [Triticum aestivum] GI: 6691467; contains Pfam profile |
| PF00226: DnaJ domain (68415.m00099) | |||||
| 376 | Not regulated | NC-NC-NC | 265867_at | At2g01620 | expressed protein (68415.m00088) |
| 377 | Not regulated | NC-NC-NC | 265868_at | At2g01650 | zinc finger (C2H2 type) family protein contains Pfam domain, PF00096: Zinc finger. C2H2 type (68415.m00091) |
| 378 | Not regulated | NC-NC-NC | 265874_at | At2g01640 | expressed protein (68415.m00090) |
| 379 | Not regulated | NC-NC-NC | 265683_at | At2g42310 | expressed protein (68415.m05237) |
| 380 | Not regulated | NC-NC-NC | 265852_at | At2g42350 | zinc finger (C3HC4-type RING finger) family protein contains Pfam domain, PF00097; Zinc finger, C3HC4 type (RING finger) |
| (68415.m05241) | |||||
| 381 | Not regulated | NC-NC-NC | 265855_at | At2g42390 | protein kinase C substrate, heavy chain-related similar to Protein kinase C substrate, 80 kDa protein, heavy chain (PKCSH) (80K-H |
| protein) (Swiss-Prot: P14314) [Homo sapiens]; contains 1 transmembrane domain; (68415.m05246) | |||||
| 382 | Not regulated | NC-NC-NC | 265857_s_at | At2g42500 | serine/threonine protein phosphatase PP2A-3 catalytic subunit (PP2A3) identical to SP|Q07100 Serine/threonine protein |
| phosphatase PP2A-3 catalytic subunit (EC 3.1.3.18) {Arabidopsis thaliana}; contains Pfam profile PF00149: Ser/Thr protein | |||||
| phosphatase (68415.m05258) | |||||
| 383 | Not regulated | NC-NC-NC | 265844_at | At2g35620 | leucine-rich repeat transmembrane protein kinase, putative similar to somatic embryogenesis receptor-like kinase 1 (SERK1) [Zea |
| mays] gi|13897318|emb|CAC37640; contains leucine rich repeat (LRR) domains, Pfam: PF00560; contains protein kinase domain, | |||||
| Pfam: PF00069 (68415.m04368) | |||||
| 384 | Not regulated | NC-NC-NC | 265825_at | At2g35635 | ubiquitin family protein contains INTERPRO: IPR000626 ubiquitin domain (68415.m04370) |
| 385 | Not regulated | NC-NC-NC | 265826_at | At2g35795 | DNAJ heat shock N-terminal domain-containing protein contains Pfam profile PF00226 DnaJ domain (68415.m04394) |
| serine carboxypeptidase S10 family protein similar to Serine carboxypeptidase II chains A and B (SP: P08819) (EC 3.4.16.6) | |||||
| 386 | Not regulated | NC-NC-NC | 265795_at | At2g35780 | [Triticum aestivum (Wheat)]; (68415.m04390) |
| 387 | Not regulated | NC-NC-NC | 265798_at | At2g35790 | expressed protein (68415.m04392) |
| 388 | Not regulated | NC-NC-NC | 265800_at | At2g35630 | microtubule organization 1 protein (MOR1) identical to microtubule organization 1 protein GI: 14317953 from [Arabidopsis thaliana] |
| (68415.m04369) | |||||
| 389 | Not regulated | NC-NC-NC | 265805_s_at | At2g18020 | 60S ribosomal protein L8 (RPL8A) (68415.m02094) |
| 390 | Not regulated | NC-NC-NC | 265810_at | At2g18090 | PHD finger family protein/SWIB complex BAF60b domain-containing protein/GYF domain-containing protein contains Pfam |
| profiles PF02201: BAF60b domain of the SWIB complex, PF02213: GYF domain, PF00628: PHD-finger (68415.m02103) | |||||
| 391 | Not regulated | NC-NC-NC | 265816_s_at | At2g18110 | elongation factor 1-beta, putative/EF-1-beta, putative nearly identical to eEF-1beta [Arabidopsis thaliana] GI: 398606 |
| (68415.m02105) | |||||
| 392 | Not regulated | NC-NC-NC | 265818_at | At2g18040 | peptidyl-prolyl cis-trans isomerase (PIN1)/cyclophilin/rotamase identical to Chain A, Solution Structure Of Pin1at From |
| 393 | Not regulated | NC-NC-NC | 265819_at | At2g17972 | Arabidopsis Thaliana GI: 22218833; contains Pfam profile PF00639: PPIC-type PPIASE domain (68415.m02097) |
| expressed protein (68415.m02086) | |||||
| 394 | Not regulated | NC-NC-NC | 265768_at | At2g48020 | sugar transporter, putative similar to ERD6 protein {Arabidopsis thaliana} GI: 3123712, sugar-porter family proteins 1 and 2 |
| [Arabidopsis thaliana] GI: 14585699, GI: 14585701: contains Pfam profile PF00083: major facilitator superfamily protein | |||||
| (68415.m06010) | |||||
| 395 | Not regulated | NC-NC-NC | 265770_at | At2g48100 | exonuclease family protein contains Pfam domain PF00929: exonuclease (68415.m06020) |
| 396 | Not regulated | NC-NC-NC | 265773_at | At2g48070 | expressed protein (68415.m06016) |
| 397 | Not regulated | NC-NC-NC | 265776_at | At2g07340 | prefoldin-related KE2 family protein contains similarity to Swiss-Prot: O60925 prefoldin subunit 1 [Homo sapiens]; contains Pfam |
| domain, PF01920: KE2 family protein (68415.m00841) | |||||
| 398 | Not regulated | NC-NC-NC | 265777_at | At2g07350 | |
| 399 | Not regulated | NC-NC-NC | 265790_at | At2g01170 | amino acid permease family protein weak similarity to GABA permease [Emericella nidulans] GI: 4972245; contains Pfam profile |
| PF00324: Amino add permease (68415.m00025) | |||||
| 400 | Not regulated | NC-NC-NC | 265736_at | At2g01250 | 60S ribosomal protein L7 (RPL7B) (68415.m00037) |
| 401 | Not regulated | NC-NC-NC | 265739_at | At2g01270 | thioredoxin family protein low similarity to quiescin [Homo sapiens] GI: 13257405; contains Pfam profiles PF00065: Thioredoxin, |
| PF04777: Erv1/Alr family (68415.m00040) | |||||
| 402 | Not regulated | NC-NC-NC | 265740_at | At2g01150 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) |
| (68415.m00024) | |||||
| 403 | Not regulated | NC-NC-NC | 265741_at | At2g01320 | ABC transporter family protein (68415.m00046) |
| 404 | Not regulated | NC-NC-NC | 265742_at | At2g01290 | expressed protein (68415.m00043) |
| 405 | Not regulated | NC-NC-NC | 265759_at | At2g13100 | glycerol-3-phosphate transporter, putative/glycerol 3-phosphate permease, putative similar to glycerol-3-phosphate transporter |
| (glycerol 3-phosphate permease) [Homo sapiens] GI: 7543982; contains Pfam profile PF00083: major facilitator superfamily protein | |||||
| (68415.m01436) | |||||
| 406 | Not regulated | NC-NC-NC | 265703_at | At2g03430 | ankyrin repeat family protein contains ankyrin repeats, Pfam: PF00023 (68415.m00301) |
| 407 | Not regulated | NC-NC-NC | 265704_at | At2g03420 | expressed protein (68415.m00300) |
| 408 | Not regulated | NC-NC-NC | 265707_at | At2g03390 | uvrB/uvrC motif-containing protein contains pfam profile PF02151: UvrB/uvrC motif (68415.m00298) |
| 409 | Not regulated | NC-NC-NC | 265712_s_at | At2g03330 | expressed protein (68415.m00292) |
| 410 | Not regulated | NC-NC-NC | 265716_at | At2g03350 | expressed protein contains Pfam profile PF04398: Protein of unknown function, DUF538 (68415.m00294) |
| 411 | Not regulated | NC-NC-NC | 265720_at | At2g40110 | yippee family protein similar to mdgl-1 [Mus musculus] GI: 10441648, Yippee protein [Drosophila melanogaster] GI: 5713279; |
| contains Pfam profile PF03226: Yippee putative zinc-binding protein (68415.m04930) | |||||
| 412 | Not regulated | NC-NC-NC | 265722_at | At2g40100 | chlorophyll A-B binding protein (LHCB4.3) identical to Lhcb4: 3 protein [Arabidopsis thaliana] GI: 4741956; contains Pfam profile: |
| PF00504 chlorophyll A-B binding protein (68415.m04929) | |||||
| 413 | Not regulated | NC-NC-NC | 265697_at | At2g32170 | expressed protein; supported by cDNA GI: 20259498 (68415.m03932) |
| 414 | Not regulated | NC-NC-NC | 265669_at | At2g32040 | integral membrane transporter family protein contains 9 transmembrane domains; contains Pfam PF03092: BT1 family; contains |
| TIGRFAMS TIGR00788: folate/biopterin transporter, similar to high affinity folic acid/methotrexate transporter 5 (GI: 21898554) | |||||
| [Leishmania tarentolae] (68415.m03914) | |||||
| 415 | Not regulated | NC-NC-NC | 265671_at | At2g32060 | 40S ribosomal protein S12 (RPS12C) (68415.m03916) |
| 416 | Not regulated | NC-NC-NC | 265673_at | At2g32090 | lactoylglutathione lyase family protein/glyoxalase I family protein contains glyoxalase family protein domain, Pfam: PF00903 |
| (68415.m03922) | |||||
| 417 | Not regulated | NC-NC-NC | 265676_at | At2g32070 | CCR4-NOT transcription complex protein, putative similar to SWISS-PROT: Q60809 CCR4-NOT transcription complex, subunit 7 |
| (CCR4-associated factor 1, (CAF1) [Mus musculus] (68415.m03919) | |||||
| 418 | Not regulated | NC-NC-NC | 265677_at | At2g32080 | PUR alpha-1 protein identical to PUR alpha-1 GI: 5081612 from [Arabidopsis thaliana]; contains Pfam profile: PF04845 PurA |
| ssDNA and RNA-binding protein (68415.m03920) | |||||
| 419 | Not regulated | NC-NC-NC | 265679_at | At2g32240 | expressed protein contains Pfam profile: PF04508 viral A-type inclusion protein repeat (68415.m03940) |
| 420 | Not regulated | NC-NC-NC | 265685_at | At2g24430 | no apical meristem (NAM) family protein contains Pfam PF02365: No apical meristem (NAM) domain; (68415.m02919) |
| 421 | Not regulated | NC-NC-NC | 265691_at | At2g24330 | expressed protein (68415.m02908) |
| 422 | Not regulated | NC-NC-NC | 265663_at | At2g24290 | expressed protein (68415.m02903) |
| 423 | Not regulated | NC-NC-NC | 265664_at | At2g24420 | DNA repair ATPase-related contains 2 transmembrane domains; similar to DNA double-strand break repair rad50 ATPase. (Swiss- |
| Prot: O33600) [Sulfolobus acidocaldarius] (68415.m02917) | |||||
| 424 | Not regulated | NC-NC-NC | 265637_at | At2g27490 | dephospho-CoA kinase family similar to Dephospho-CoA kinase (EC 2.7.1.24) (Dephosphocoenzyme A kinase). (Swiss- |
| Prot: Q92BF2) [Listeria innocua]; contains Pfam profile PF01121: Dephospho-CoA kinase (68415.m03322) | |||||
| 425 | Not regulated | NC-NC-NC | 265642_at | At2g27450 | carbon-nitrogen hydrolase family protein low similarity to beta-alanine synthase [Drosophila melanogaster] GI: 14334063; contains |
| Pfam profile PF00795: hydrolase, carbon-nitrogen family (68415.m03317) | |||||
| 426 | Not regulated | NC-NC-NC | 265646_at | At2g27360 | lipase, putative similar to lipase [Arabidopsis thaliana] GI: 1145627; contains Pfam profile PF00657: Lipase/Acylhydrolase with |
| GDSL-like motif (68415.m03296) | |||||
| 427 | Not regulated | NC-NC-NC | 265648_at | At2g27500 | glycosyl hydrolase family 17 protein similar to glucan endo-1,3-beta-glucosidase precursor SP: P52409 from [Triticum aestivum] |
| (68415.m03324) | |||||
| 428 | Not regulated | NC-NC-NC | 265649_at | At2g27510 | ferredoxin, putative similar to non-photosynthetic ferredoxin from Citrus sinensis [GI: 1360725], Ferredoxin, root R-B2 from |
| Raphanus sativus [SP|P14937]; contains Pfam profile PF00111 2Fe—2S iron-sulfur cluster binding domain (68415.m03327) | |||||
| 429 | Not regulated | NC-NC-NC | 265617_at | At2g25520 | phosphate translocator-related low similarity to SP|P52178 Triose phosphate/phosphate translocator, non-green plastid, chloroplast |
| precursor (CTPT) {Brassica oleracea}, phosphoenolpyruvate/phosphate translocator precursor [Mesembryanthemum crystallinum] | |||||
| GI: 9295275 (68415.m03055) | |||||
| 430 | Not regulated | NC-NC-NC | 265626_at | At2g27260 | expressed protein (68415.m03276) |
| 431 | Not regulated | NC-NC-NC | 265628_at | At2g27290 | expressed protein (68415.m03280) |
| 432 | Not regulated | NC-NC-NC | 265630_at | At2g27350 | OTU-like cysteine protease family protein contains Pfam profile PF02338: OTU-like cysteine protease (68415.m03291) |
| 433 | Not regulated | NC-NC-NC | 265575_at | At2g14260 | proline iminopeptidase identical to GP: 1710151: U72711 (68415.m01594) |
| 434 | Not regulated | NC-NC-NC | 265593_s_at | At2g20130 | expressed protein contains Pfam domain, PF04367: Protein of unknown function (DUF502) (68415.m02352) |
| 435 | Not regulated | NC-NC-NC | 265594_at | At2g20060 | ribosomal protein L4 family protein contains Pfam profile PF00573: ribosomal protein L4/L1 family (68415.m02344) |
| 436 | Not regulated | NC-NC-NC | 265595_at | At2g20140 | 26S protease regulatory complex subunit 4, putative similar to Swiss-Prot: P48601 26S protease regulatory subunit 4 (P26S4) |
| [Drosophila melanogaster] (68415.m02353) | |||||
| 437 | Not regulated | NC-NC-NC | 265571_s_at | At2g28230 | expressed protein (68415.m03427) |
| 438 | Not regulated | NC-NC-NC | 265573_at | At2g26200 | zinc finger (C2H2 type) family protein contains Pfam domain, PF00096: Zinc finger, C2H2 type (68415.m03424) |
| 439 | Not regulated | NC-NC-NC | 265569_at | At2g05620 | expressed protein (68415.m00598) |
| 440 | Not regulated | NC-NC-NC | 265510_at | At2g05630 | autophagy 8d (APG8d) identical to autophagy 8d [Arabidopsis thaliana] GI: 19912157; contains Pfam profile PF02991: Microtubule |
| associated protein 1A/1B, light chain 3 (68415.m00599) | |||||
| 441 | Not regulated | NC-NC-NC | 265538_at | At2g15860 | expressed protein and genefinder (68415.m01818) |
| 442 | Not regulated | NC-NC-NC | 265495_at | At2g15695 | expressed protein contains Pfam PF05705: Eukaryotic protein of unknown function (DUF829) (68415.m01797) |
| 443 | Not regulated | NC-NC-NC | 265474_at | At2g15690 | pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat (68415.m01796) |
| 444 | Not regulated | NC-NC-NC | 265476_at | At2g15570 | thioredoxin M-type 3, chloroplast (TRX-M3) identical to SP|Q9SEU7 Thioredoxin M-type 3, chloroplast precursor (TRX-M3) |
| {Arabidopsis thaliana} (68415.m01783) | |||||
| 445 | Not regulated | NC-NC-NC | 265450_at | At2g46620 | AAA-type ATPase family protein contains Pfam profile: ATPase family PF00004 (68415.m05815) |
| 446 | Not regulated | NC-NC-NC | 265456_at | At2g46505 | expressed protein (68415.m05794) |
| 447 | Not regulated | NC-NC-NC | 265457_at | At2g46550 | expressed protein (68415.m05807) |
| 448 | Not regulated | NC-NC-NC | 265458_at | At2g46580 | pyridoxine 5′-phosphate oxidase-related similar to pyridoxine 5′-phosphate oxidase (GI: 3237304) [Rattus norvegicus]; similar to |
| pyridoxamine 5′-phosphate oxidase (GI: 22294052) [Thermosynechococcus elongatus BP-1] (68415.m05810) | |||||
| 449 | Not regulated | NC-NC-NC | 265461_at | At2g46500 | phosphatidylinositol 3- and 4-kinase family protein/ubiquitin family protein contains INTERPRO: IPR000626 ubiquitin domain |
| (68415.m05790) | |||||
| 450 | Not regulated | NC-NC-NC | 265471_at | At2g37130 | peroxidase 21 (PER21) (P21) (PRXR5) identical to SP|Q42580 Peroxidase 21 precursor (EC 1.11.1.7) (Atperox P21) (PRXR5) |
| (ATP2a/ATP2b) {Arabidopsis thaliana} (68415.m04555) | |||||
| 451 | Not regulated | NC-NC-NC | 265416_at | At2937120 | DNA-binding S1FA family protein contains Pfam profile; PF04689 DNA binding protein S1FA (68415.m04554) |
| 452 | Not regulated | NC-NC-NC | 265417_at | At2g20920 | expressed protein (68415.m02467) |
| 453 | Not regulated | NC-NC-NC | 265419_at | At2g20840 | secretory carrier membrane protein (SCAMP) family protein contains Pfam domain, PF04144: SCAMP family (68415.m02456) |
| 454 | Not regulated | NC-NC-NC | 265426_at | At2g20740 | expressed protein (68415.m02436) |
| 455 | Not regulated | NC-NC-NC | 265442_at | At2g20940 | expressed protein (68415.m02469) |
| 456 | Not regulated | NC-NC-NC | 265415_at | At2g20890 | expressed protein (68415.m02462) |
| 457 | Not regulated | NC-NC-NC | 265385_at | At2g20900 | diacylglycerol kinase, putative contains INTERPRO domain, IPR001206, DAG-kinase catalytic domain (68415.m02463) |
| 458 | Not regulated | NC-NC-NC | 265386_at | At2g20930 | expressed protein (68415.m02468) |
| 459 | Not regulated | NC-NC-NC | 265390_at | At2g20820 | expressed protein (68415.m02450) |
| 460 | Not regulated | NC-NC-NC | 265391_at | At2g20810 | glycosyl transferase family 6 protein contains Pfam profile: PF01501 glycosyl transferase family 6 (68415.m02448) |
| 461 | Not regulated | NC-NC-NC | 265392_at | At2g20860 | lipoic acid synthase (LIP1) identical to gi: 3928758 contains Pfam profile PF04055: radical SAM domain protein (68415.m02458) |
| 462 | Not regulated | NC-NC-NC | 265393_at | At2g20830 | expressed protein weak similarity to formiminotransferase cyclodeaminase (GI: 3980064) [Gallus gallus] (68415.m02453) |
| 463 | Not regulated | NC-NC-NC | 265394_at | At2g20725 | CAAX amino terminal protease family protein contains Pfam profile PF02517 CAAX amino terminal protease family protein |
| (68415.m02435) | |||||
| 464 | Not regulated | NC-NC-NC | 265402_at | At2g10950 | BSD domain-containing protein contains Pfam profile PF03909: BSD domain (68415.m01169) |
| 465 | Not regulated | NC-NC-NC | 265382_at | At2g16790 | shikimate kinase family protein similar to thermosensitive glucokinase from Escherichia coli [SP|P39208] [SP|P46859]; contains |
| Pfam profile PF01202: shikimate kinase (68415.m01925) | |||||
| 466 | Not regulated | NC-NC-NC | 265352_at | At2g16600 | peptidyl-prolyl cis-trans isomerase, cytosolic/cydophilin/rotamase (ROC3) identical to cytosotic cyclophilin [Arabidopsis thaliana] |
| GI: 1305455 (68415.m01906) | |||||
| 467 | Not regulated | NC-NC-NC | 265354_at | At2g16700 | actin-depolymerizing factor 5 (ADF5) identical to SP|Q9ZNT3 Actin-depolymerizing factor 5 (ADF-5) (AtADF5) {Arabidopsis |
| thaliana} (68415.m01916) | |||||
| 468 | Not regulated | NC-NC-NC | 265356_at | At2g16595 | translocon-associated protein (TRAP), putative similar to Swiss-Prot: P45434 translocon-associated protein, alpha subunit precursor |
| (TRAP-alpha, Signal sequence receptor alpha subunit, SSR-alpha) [Arabidopsis thaliana] (68415.m01904) | |||||
| 469 | Not regulated | NC-NC-NC | 265357_at | At2g16740 | ubiquilin-conjugating enzyme, putative strong similarity to SP|P35133 Ubiquitin-conjugating enzyme E2-17 kDa 10 (EC 6.3.2.19) |
| (Ubiquitin-protein ligase 10) (Ubiquitin carrier protein 10) (Arabidopsis thaliana); contains Pfam profile PF00179: Ubiquitin- | |||||
| conjugating enzyme (68415.m01920) | |||||
| 470 | Not regulated | NC-NC-NC | 265374_at | At2g06520 | membrane protein, putative contains 2 transmembrane domains; (68415.m00723) |
| 471 | Not regulated | NC-NC-NC | 265375_at | At2g06530 | SNF7 family protein contains Pfam domain, PF03357: SNF7 family (68415.m00724) |
| 472 | Not regulated | NC-NC-NC | 265334_at | At2g18370 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein similar to lipid-transfer protein [Nicotiana glauca] |
| GI: 6782436; contains Pfam protease inhibitor/seed storage/LTP family domain PF00234 (68415.m02140) | |||||
| 473 | Not regulated | NC-NC-NC | 265338_at | At2g18400 | ribosomal protein L6 family protein (68415.m02143) |
| 474 | Not regulated | NC-NC-NC | 265340_at | At2g18330 | AAA-type ATPase family protein contains Pfam profile: PF00004 ATPase family associated with various cellular activities (AAA) |
| (68415.m02136) | |||||
| 475 | Not regulated | NC-NC-NC | 265319_at | At2g22670 | auxin-responsive protein/indoleacetic acid-induced protein 8 (IAA8) identical to SP|Q38826 Auxin-responsive protein IAA8 |
| (Indoleacetic acid-induced protein 8) {Arabidopsis thaliana} (68415.m02686) | |||||
| 476 | Not regulated | NC-NC-NC | 265289_at | At2g22690 | expressed protein (68415.m02689) |
| 477 | Not regulated | NC-NC-NC | 265291_at | At2g22720 | expressed protein (68415.m02691) |
| 478 | Not regulated | NC-NC-NC | 265307_at | At2g20310 | expressed protein (68415.m02372) |
| 479 | Not regulated | NC-NC-NC | 265284_at | At2g20230 | expressed protein (68415.m02364) |
| 480 | Not regulated | NC-NC-NC | 265288_at | At2g20270 | glutaredoxin family protein contains glutaredoxin domain, Pfam: PF00462 (68415.m02368) |
| 481 | Not regulated | NC-NC-NC | 265258_at | At2g20390 | expressed protein (68415.m02380) |
| 482 | Not regulated | NC-NC-NC | 265259_at | At2g20330 | transducin family protein/WD-40 repeat family protein similar to Transcriptional repressor rco-1 (SP: P78706) [Neurospora crassa]; |
| similar to TUP1(GB: AF079369); contains 6 WD-40 repeats (PF00400) (68415.m02374) | |||||
| 483 | Not regulated | NC-NC-NC | 265265_at | At2g42900 | expressed protein (68415.m05314) |
| 484 | Not regulated | NC-NC-NC | 265266_at | At2g42890 | RNA recognition motif (RRM)-containing protein (68415.m05311) |
| 485 | Not regulated | NC-NC-NC | 265270_at | At2g42910 | ribose-phosphate pyrophosphokinase 4/phosphoribosyl diphosphate synthetase 4 (PRS4) identical to phosphoribosyl |
| diphosphate synthase (prs4) [Arabidopsis thaliana] GI: 4902472 (68415.m05316) | |||||
| 486 | Not regulated | NC-NC-NC | 265274_at | At2g28450 | zinc finger (CCCH-type) family protein contains Pfam domain, PF00842: Zinc finger C-x8-C-x5-C-x3-H type (and similar) |
| (68415.m03456) | |||||
| 487 | Not regulated | NC-NC-NC | 265281_at | At2g28370 | expressed protein contains Pfam PF04535: Domain of unknown function (DUF588); similar to putative ethylene responsive |
| element binding protein (GI: 22135858) [Arabidopsis thaliana] (68415.m03448) | |||||
| 488 | Not regulated | NC-NC-NC | 265256_at | At2g28390 | SAND family protein similar to Sand (GI: 3928166) [Takifugu rubripes]; contains Pfam PF03164: SAND family protein |
| (68415.m03450) | |||||
| 489 | Not regulated | NC-NC-NC | 265226_at | At2g28430 | expressed protein (68415.m03454) |
| 490 | Not regulated | NC-NC-NC | 265228_s_at | At2g07698 | ATP synthase alpha chain, mitochondrial, putative very strong similarity to SP|P23413 ATP synthase alpha chain, mitochondrial |
| (EC 3.6.3.14) {Brassica campestris}; contains Pfam profiles PF00006: ATP synthase alpha/beta family nucleotide-binding domain, | |||||
| PF00306: ATP synthase ab C terminal, PF02874: ATP synthase alpha/beta family beta-barrel domain (68415.m00949) | |||||
| 491 | Not regulated | NC-NC-NC | 265230_s_at | At2g07707 | hypothetical protein contains Pfam profile PF02328: YMF19 hypothetical plant mitochondrial protein (68415.m00957) |
| 492 | Not regulated | NC-NC-NC | 285247_at | At2g43030 | ribosomal protein L3 family protein contains Pfam profile PF00297: ribosomal protein L3 (68415.m05340) |
| 493 | Not regulated | NC-NC-NC | 265248_at | At2g43010 | phytochrome-interacting factor 4 (PIF4)/basic helix-loop-helix protein 9 (bHLH9)/short under red-light 2 (SRL2) identical to |
| SP|Q8W2F3 Phytochrome-interacting factor 4 (Basic helix-loop-helix protein 9) (bHLH9) (Short under red-light 2) {Arabidopsis | |||||
| thaliana}; supporting cDNA gi|18026965|gb|AF251694.1|AF251694 (68415.m05337) | |||||
| 494 | Not regulated | NC-NC-NC | 265252_at | At2g01980 | sodium proton exchanger, putative (NHX7) (SOS1) identical to putative Na+/H+ antiporter SOS1 [Arabidopsis thaliana] |
| gi|8515714|gb|AAF76139; Member of The Monovalent Cation:Proton Antiporter (CPA1) Family, PMID: 11500563 (68415.m00133) | |||||
| 495 | Not regulated | NC-NC-NC | 265219_at | At2g02050 | NADH-ubiquinone oxidoreductase B18 subunit, putative contains Pfam PF05676: NADH-ubiquinone oxidoreductase B18 subunit |
| (NDUFB7); similar to NADH-ubiquinone oxidoreductase B18 subunit (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-B18) (CI-B18) (Cell | |||||
| adhesion protein SQM1) (Swiss-Prot: P17568) [Homo sapiens]; similar to NADH:ubiquinoe oxidoreductase NDUFB7 subunit | |||||
| (GI: 9651635) [Homo sapiens] (68415.m00140) | |||||
| 496 | Not regulated | NC-NC-NC | 265220_at | At2g02040 | peptide transporter (PTR2-B)/oligopeptide transporter 1-1, putative (OPT1-1) identical to peptide transporter PTR2-B SP: P46032 |
| from [Arabidopsis thaliana]; contains Pfam profile: PF00854 POT family; identical to cDNA NT1 GI: 510237 (68415.m00139) | |||||
| 497 | Not regulated | NC-NC-NC | 265203_at | At2g38630 | expressed protein contains Pfam profile: PF01925 domain of unknown function DUF81 (68415.m04492) |
| 498 | Not regulated | NC-NC-NC | 265210_at | At2g38620 | 60S ribosomal protein L24 (RPL24A) (68415.m04490) |
| 499 | Not regulated | NC-NC-NC | 265212_at | At1g05030 | hexose transporter, putative similar to hexose transporters from Nicotiana tabacum (GI: 8347244), Solanum tuberosum |
| (GI: 8347246), Arabidopsis thaliana (GI: 8347250); contains Pfam profile PF00083: major facilitator superfamily protein | |||||
| (68414.m00504) | |||||
| 500 | Not regulated | NC-NC-NC | 265214_at | At1g05000 | tyrosine specific protein phosphatase family protein contains tyrosine specific protein phosphatases active site, PROSITE: PS00383 |
| (68414.m00501) | |||||
| 501 | Not regulated | NC-NC-NC | 265218_at | At1g05050 | |
| 502 | Not regulated | NC-NC-NC | 265192_at | At1g05060 | expressed protein (68414.m00507) |
| 503 | Not regulated | NC-NC-NC | 265193_at | At1g05070 | expressed protein (68414.m00509) |
| 504 | Not regulated | NC-NC-NC | 265172_at | At1g23820 | spermidine synthase 1 (SPDSYN1)/putrescine aminopropyltransferase 1 identical to SP|Q9ZUB3 Spermidine synthase 1 (EC |
| 2.5.1.16) (Putrescine aminopropyltransferase 1) (SPDSY 1) {Arabidopsis thaliana} (68414.m03004) | |||||
| 505 | Not regulated | NC-NC-NC | 265183_at | At1g23750 | DNA-binding protein-related contains weak similarity to G-quartet DNA binding protein 3 [Tetrahymena thermophila] |
| gi|4583503|gb|AAD25098 (68414.m02997) | |||||
| 506 | Not regulated | NC-NC-NC | 265184_at | At1g23710 | expressed protein (68414.m02993) |
| 507 | Not regulated | NC-NC-NC | 265187_at | At1g23490 | ADP-ribosylation factor identical to SP: Q9SRC3 ADP-ribosylation factor 1-like [Arabidopsis thaliana], ADP-ribosylation factor |
| GI: 166586 [Arabidopsis thaliana] (68414.m02948) | |||||
| 508 | Not regulated | NC-NC-NC | 265188_at | At1g23800 | aldehyde dehydrogenase, mitochondrial (ALDH3) nearly identical to mitochondrial aldehyde dehydrogenase ALDH3 [Arabidopsis |
| thaliana] gi|19850249|gb|AAL99612; contains Pfam profile PF00171: aldehyde dehydrogenase (NAD) family protein | |||||
| (68414.m03002) | |||||
| 509 | Not regulated | NC-NC-NC | 265190_at | At1g23780 | F-box family protein contains Pfam PF00646: F-box domain: similar to SP: Q9Y3I1 F-box only protein 7 {Homo sapiens}; similar to |
| SKP1 interacting partner 2 (SKIP2) TIGR_Ath1: At5g67250 (68414.m03000) | |||||
| 510 | Not regulated | NC-NC-NC | 265132_at | At1g23830 | expressed protein (68414.m03006) |
| 511 | Not regulated | NC-NC-NC | 265148_at | At1g51160 | synbindin, putative similar to Swiss-Prot: Q9ES56 synbindin (TRS23 homolog) [Mus musculus] (68414.m05752) |
| 512 | Not regulated | NC-NC-NC | 265150_at | At1g51200 | zinc finger (AN1-like) family protein contains Pfam domains, PF01428: AN1-like Zinc finger and PF01754: A20-like zinc finger |
| (68414.m05759) | |||||
| 513 | Not regulated | NC-NC-NC | 265162_at | At1g30910 | molybdenum cofactor sulfurase family protein weak similarity to molybdenum cofactor sulfurase (LOS5/ABA3) [Arabidopsis |
| thaliana] GI: 15407262; contains Pfam profiles PF03476: MOSC N-terminal beta barrel domain, PF03473: MOSC domain | |||||
| (68414.m03781) | |||||
| 514 | Not regulated | NC-NC-NC | 265129_at | At1g30970 | zinc finger (C2H2 type) family protein contains Pfam domain PF00096: Zinc finger, C2H2 type (68414.m03792) |
| 515 | Not regulated | NC-NC-NC | 265130_at | At1g30890 | integral membrane HRF1 family protein contains Pfam domain PF03878: Hrf1 family (68414.m03779) |
| 516 | Not regulated | NC-NC-NC | 265101_at | At1g30880 | expressed protein similar to ESTs gb|R30049 and gb|T46176 (68414.m03778) |
| 517 | Not regulated | NC-NC-NC | 265102_at | At1g30870 | cationic peroxidase, putative similar to cationic peroxidase (gi|1232069); similar to EST gb|AI100412 (68414.m03776) |
| 518 | Not regulated | NC-NC-NC | 265113_at | At1g62460 | |
| 519 | Not regulated | NC-NC-NC | 265117_at | At1g62500 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein similar to auxin down regulated GB: X69640 GI: 296442 |
| from [Glycine max]; contains Pfam profile PF00234: Protease inhibitor/seed storage/LTP family (68414.m07052) | |||||
| 520 | Not regulated | NC-NC-NC | 265121_at | At1g62560 | flavin-containing monooxygenase family protein/FMO family protein similar to flavin-containing monooxygenase GB: AAA21178 |
| GI: 349534 SP|P32417 from [Oryctolagus cuniculus]; contains Pfam profile PF00743 Flavin-binding monooxygenase-like | |||||
| (68414.m07058) | |||||
| 521 | Not regulated | NC-NC-NC | 265071_at | At1g55520 | transcription initiation factor IID-2 (TFIID-2)/TATA-box factor 2/TATA sequence-binding protein 2 (TBP2) identical to Swiss- |
| Prot: P28148|TF22_ARATH Transcription initiation factor TFIID-2 (TATA-box factor 2) (TATA sequence-binding protein 2) (TBP-2) | |||||
| [Arabidopsis thaliana] (68414.m06351) | |||||
| 522 | Not regulated | NC-NC-NC | 265073_at | At1g55480 | expressed protein (68414.m06346) |
| 523 | Not regulated | NC-NC-NC | 265092_at | At1g03910 | expressed protein low similarity to cactin [Drosophila melanogaster] GI: 7673675; expression supported by MPSS (68414.m00376) |
| 524 | Not regulated | NC-NC-NC | 265036_at | At1g04080 | hydroxyproline-rich glycoprotein family protein Contains similarity to pre-mRNA processing protein PRP39 gp L29224 from |
| S. cerevisiae. ESTs gb|R64908 and gb|T88158, gb|N38703 and gb|AA651043 come from this gene (68414.m00396) | |||||
| 525 | Not regulated | NC-NC-NC | 265037_at | At1g03860 | prohibitin, putative similar to SP|P24142 Prohibitin (B-cell receptor associated protein 32) (BAP 32) {Rattus norvegicus}; contains |
| Pfam profile PF01145: SPFH domain/Band 7 family (68414.m00368) | |||||
| 526 | Not regulated | NC-NC-NC | 265043_at | At1g03900 | expressed protein (68414.m00374) |
| 527 | Not regulated | NC-NC-NC | 265048_at | At1g52050 | jacalin lectin family protein similar to myrosinase-binding protein homolog [Arabidopsis thaliana] GI: 2997767; contains Pfam profile |
| PF01419 jacalin-like lectin domain (68414.m05872) | |||||
| 528 | Not regulated | NC-NC-NC | 265057_at | At1g52140 | expressed protein (68414.m05883) |
| 529 | Not regulated | NC-NC-NC | 265032_at | At1g61580 | 60S ribosomal protein L3 (RPL3B) identical to ribosomal protein GI: 806279 from [Arabidopsis thaliana] (68414.m06939) |
| 530 | Not regulated | NC-NC-NC | 265033_at | At1g61520 | chlorophyll A-B binding protein/LHCI type III (LHCA3.1) nearly identical to PSI type III chlorophyll a/b-binding protein GI: 430947; |
| contains Pfam profile: PF00504 chlorophyll A-B binding protein; similar to PSI type III chlorophyll a/b-binding protein GI: 430947 | |||||
| from [Arabidopsis thaliana] (68414.m06931) | |||||
| 531 | Not regulated | NC-NC-NC | 265035_at | At1g61620 | expressed protein contains Pfam profile: PF01363 FYVE zinc finger (68414.m06943) |
| 532 | Not regulated | NC-NC-NC | 265005_at | At1g61667 | expressed protein contains Pfam profile PF04398: Protein of unknown function, DUF538 (68414.m06953) |
| 533 | Not regulated | NC-NC-NC | 265006_at | At1g61570 | mitochondrial import inner membrane translocase (TIM13) identical to mitochondrial import inner membrane translocase subunit |
| Tim13 [Arabidopsis thaliana] Swiss-Prot: Q9XH48; contains Pfam domain, PF02953: Tim10/DDP family zinc finger (68414.m06938) | |||||
| 534 | Not regulated | NC-NC-NC | 265013_at | At1g24460 | myosin-related contains TIGRFAM TIGR01612: reticulocyte binding protein; similar to Myosin heavy chain, non-muscle (Zipper |
| protein) (Myosin II) (SP: Q99323) {Drosophila melanogaster} similar to EST gb|T76116 (68414.m03081) | |||||
| 535 | Not regulated | NC-NC-NC | 265014_at | At1g24430 | transferase family protein similar to deacetylvindoline 4-O-acetyltransferase from Catharanthus roseus GI: 4091808 GB: AAC99311, |
| acetyl CoA:benzylalcohol acetyltransferase Clarkia breweri GI: 3170250, acetyl-CoA:benzylalcohol acetyltranferase Clarkia | |||||
| concinna GI: 6166328: contains Pfam profile PF02458 transferase family (68414.m03076) | |||||
| 536 | Not regulated | NC-NC-NC | 265021_at | At1g24610 | SET domain-containing protein low similarity to SP|Q43088 Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N- |
| methyltransferase, chloroplast precursor (EC 2.1.1.127) {Pisum sativum}; contains Pfam profile PF00856: SET domain | |||||
| (68414.m03096) | |||||
| 537 | Not regulated | NC-NC-NC | 265025_at | At1g24575 | expressed protein (68414.m03093) |
| 538 | Not regulated | NC-NC-NC | 265027_at | At1g24450 | ribonuclease III family protein contains similarity to Swiss-Prot: P51837 ribonuclease III (EC 3.1.26.3) (RNase III) [Coxiella burnetii] |
| (68414.m03080) | |||||
| 539 | Not regulated | NC-NC-NC | 264977_at | At1g27090 | glycine-rich protein (68414.m03302) |
| 540 | Not regulated | NC-NC-NC | 264980_at | At1g27190 | leucine-rich repeat transmembrane protein kinase, putative similar to CLV1 receptor kinase GB: AAB58929 GI: 2160756 from |
| [Arabidopsis thaliana] (68414.m03312) | |||||
| 541 | Not regulated | NC-NC-NC | 264984_at | At1g27000 | bZIP family transcription factor (68414.m03292) |
| 542 | Not regulated | NC-NC-NC | 264990_at | At1g27210 | expressed protein (68414.m03314) |
| 543 | Not regulated | NC-NC-NC | 264996_at | At1g67230 | expressed protein (68414.m07652) |
| 544 | Not regulated | NC-NC-NC | 264998_at | At1g67330 | expressed protein contains Pfam profile PF04669: Protein of unknown function (DUF579) (68414.m07664) |
| 545 | Not regulated | NC-NC-NC | 265001_at | At1g67350 | expressed protein contains similarity to MHC class II antigen GI: 9502037 from [Aotus nancymaae] (68414.m07666) |
| 546 | Not regulated | NC-NC-NC | 264970_at | At1g67280 | lactoylglutathione lyase, putative/glyoxalase I, putative similar to putative lactoylglutathione lyase SP: Q39366, GI: 2494843 from |
| [Brassica oleracea] (68414.m07657) | |||||
| 547 | Not regulated | NC-NC-NC | 264942_at | At1g67340 | zinc finger (MYND type) family protein/F-box family protein (68414.m07665) |
| 548 | Not regulated | NC-NC-NC | 264944_at | At1g76940 | RNA recognition motif (RRM)-containing protein contains Pfam PF00076: RNA recognition motif. (a.k.a. RRM, RBD, or RNP |
| domain); similar to RNA-binding protein with multiple splicing homolog (RBP-MS) (HEart, RRM Expressed Sequence) (Hermes) | |||||
| (Swiss-Prot: Q9YGI5) [Xenopus laevis]; similar to RNA-binding protein with multiple splicing (RBP-MS) (Swiss-Prot: Q93062) [Homo | |||||
| sapiens] (68414.m08957) | |||||
| 549 | Not regulated | NC-NC-NC | 264952_at | At1g76900 | F-box family protein/tubby family protein similar to Tubby protein (SP: P50586) {Mus musculus}; similar to Chain A, C-Terminal |
| Domain Of Mouse Brain Tubby Protein (GI: 6730158) [Mus musculus]; similar to Tubby related protein 1 (Tubby-like protein 1) | |||||
| (Swiss-Prot: O00294) [Homo sapiens]; similar to phosphodiesterase (GI: 467578) [Mus musculus]; contains Pfam profile: PF01167: | |||||
| Tub family; contains Pfam PF00646: F-box domain (68414.m08949) | |||||
| 550 | Not regulated | NC-NC-NC | 264954_at | At1g77060 | mutase family protein similar to carboxyvinyl-carboxyphosphonate phosphorylmutase GB: O49290 from [Arabidopsis thaliana]; |
| similar to carboxyphosphonoenolpyruvate mutase (GI: 47149) [Streptomyces hygroscopicus]; contains Prosite PS00161: Isocitrate | |||||
| lyase signature (68414.m08972) | |||||
| 551 | Not regulated | NC-NC-NC | 264955_at | At1g76920 | F-box family protein (FBX3) contains similarity to stamina pistilloidia GI: 4101570, the pea ortholog of Fim and UFO from [Pisum |
| sativum] (68414.m08954) | |||||
| 552 | Not regulated | NC-NC-NC | 264959_at | At1g77090 | thylakoid lumenal 29.8 kDa protein identical to SP|O49292|TL30_ARATH (Arabidopsis thaliana); contains a PsbP domain |
| AF370571; SIMILAR TO GI: 13926195-F22K20.16 (68414.m08978) | |||||
| 553 | Not regulated | NC-NC-NC | 264916_at | At1g60810 | ATP citrate-lyase-related similar to ATP citrate-lyase GI: 949989 from [Rattus norvegicus] (68414.m06845) |
| 554 | Not regulated | NC-NC-NC | 264922_s_at | At1g60830 | U2 snRNP auxiliary factor large subunit, putative similar to GI: 3850823 from (Nicotiana plumbaginifolia) (J. Biol. Chem. 273 (51), |
| 34603-34610 (1998)) (68414.m06846) | |||||
| 555 | Not regulated | NC-NC-NC | 264928_at | At1g60710 | aldo/keto reductase family protein contains Pfam profile PF00248: oxidoreductase, aldo/keto reductase family (68414.m06834) |
| 556 | Not regulated | NC-NC-NC | 264935_at | At1g61150 | expressed protein similar to Protein C20orf11 (Swiss-Prot: Q9NWU2) [Homo sapiens] (68414.m06889) |
| 557 | Not regulated | NC-NC-NC | 264936_at | At1g61140 | SNF2 domain-containing protein/helicase domain-containing protein/zinc finger protein-related similar to ATPase [Homo sapiens] |
| GI: 531196; contains Pfam profiles PF00271: Helicase conserved C-terminal domain, PF00176: SNF2 family N-terminal domain, | |||||
| PF00097: Zinc finger, C3HC4 type (RING finger) (68414.m06888) | |||||
| 558 | Not regulated | NC-NC-NC | 264883_s_at | At1g61250 | secretory carrier membrane protein (SCAMP) family protein (SC3) contains Pfam domain, PF04144: SCAMP family |
| (68414.m06902) | |||||
| 559 | Not regulated | NC-NC-NC | 264894_at | At1g23040 | hydroxyproline-rich glycoprotein family protein contains proline-rich domains, INTERPRO: IPR000694 (68414.m02878) |
| 560 | Not regulated | NC-NC-NC | 264897_at | At1g23220 | dynein light chain type 1 family protein similar to SP|O02414 Dynein light chain LC6, flagellar outer arm {Anthocidaris crassispina}; |
| contains Pfam profile PF01221: Dynein light chain type 1 (68414.m02904) | |||||
| 561 | Not regulated | NC-NC-NC | 264904_s_at | At2g17420 | thioredoxin reductase 2/NADPH-dependent thioredoxin reductase 2 (NTR2) identical to SP|Q39242 (68415.m02010) |
| 562 | Not regulated | NC-NC-NC | 264908_at | At2g17440 | leucine-rich repeat family protein contains Pfam PF00560: Leucine Rich Repeats (68415.m02012) |
| 563 | Not regulated | NC-NC-NC | 264875_at | At2g17390 | ankyrin repeat family protein contains ankyrin repeats, Pfam: PF00023 (68415. m02008) |
| 564 | Not regulated | NC-NC-NC | 264847_at | At2g17380 | clathrin assembly protein AP19 identical to clathrin assembly protein AP19 GI: 2231698 from [Arabidopsis thaliana] (68415.m02007) |
| 565 | Not regulated | NC-NC-NC | 264848_at | At2g17350 | expressed protein (68415.m02004) |
| 566 | Not regulated | NC-NC-NC | 264850_at | At2g17340 | pantothenate kinase-related contains Pfam domain, PF01937: Protein of unknown function; supported by tandem duplication of |
| pantothenate kinase-related protein (TIGR_Ath1: At2g17320) [Arabidopsis thaliana] (68415.m02003) | |||||
| 567 | Not regulated | NC-NC-NC | 264854_at | At2g17450 | zinc finger (C3HC4-type RING finger) family protein contains Pfam domain, PF00097: Zinc finger, C3HC4 type (RING finger) |
| (68415.m02014) | |||||
| 568 | Not regulated | NC-NC-NC | 264862_at | At1g24330 | armadillo/beta-catenin repeat family protein/U-box domain-containing family protein contains Pfam domain, PF00514: |
| Armadillo/beta-catenin-like repeats and Pfam, PF04564: U-box domain (68414.m03069) | |||||
| 569 | Not regulated | NC-NC-NC | 264865_at | At1g24120 | DNAJ heat shock protein, putative similar to Altered Response to Gravity [Arabidopsis thaliana] GI: 4249662; contains Pfam profile |
| PF00226 DnaJ domain (68414.m03043) | |||||
| 570 | Not regulated | NC-NC-NC | 264869_at | At1g24350 | expressed protein contains Pfam domain, PF02681: Uncharacterized BCR, COG1963 (68414.m03071) |
| 571 | Not regulated | NC-NC-NC | 264871_at | At1g24180 | pyruvate dehydrogenase E1 component alpha subunit, mitochondrial, putative similar to SP|P52901 Pyruvate dehydrogenase E1 |
| component alpha subunit, mitochondrial precursor (EC 1.2.4.1) (PDHE1-A) {Arabidopsis thaliana}; contains Pfam profile PF00676: | |||||
| Dehydrogenase E1 component (68414.m03050) | |||||
| 572 | Not regulated | NC-NC-NC | 264818_at | At1g03530 | expressed protein similar to hypothetical protein GB: O14360 (68414.m00334) |
| 573 | Not regulated | NC-NC-NC | 264820_at | At1g03475 | coproporphyrinogen III oxidase, putative/coproporphyrinogenase, putative/coprogen oxidase, putative similar to |
| coproporphyrinogen III oxidase, chloroplast [precursor] from Glycine max [SP|P35055], Nicotiana tabacum [SP|Q42946], Hordeum | |||||
| vulgare [SP|Q42840], ESTs gb|AA586260 and dbj|D48620; contains Pfam domain coproporphyrinogen III oxidase, aerobic | |||||
| [PF01218] (68414.m00329) | |||||
| 574 | Not regulated | NC-NC-NC | 264824_at | At1g03420 | expressed protein similar to gb|T45484, emb|Z30724, and emb|Z30531 (68414.m00322) |
| 575 | Not regulated | NC-NC-NC | 264828_at | At1g03380 | expressed protein (68414.m00317) |
| 576 | Not regulated | NC-NC-NC | 264834_at | At1g03730 | expressed protein similar to ESTs gb|AA605440 and gb|H37232 (68414.m00353) |
| 577 | Not regulated | NC-NC-NC | 264836_at | At1g03610 | expressed protein (68414.m00341) |
| 578 | Not regulated | NC-NC-NC | 264837_at | At1g03600 | photosystem II family protein similar to SP: P74367 {Synechocystis sp.}; similar to ESTs emb|Z27038, gb|AA451546, emb|Z29876, |
| gb|T45359 and gp|R90316 (68414.m00340) | |||||
| 579 | Not regulated | NC-NC-NC | 264838_at | At1g03430 | two-component phosphorelay mediator, putative strong similarity to ATHP1 [Arabidopsis thatiana] GI: 4156241 (68414.m00323) |
| 580 | Not regulated | NC-NC-NC | 264840_at | At1g03440 | leucine-rich repeat family protein contains leucine rich-repeat domains Pfam: PF00560, INTERPRO: IPR001611 (68414.m00324) |
| 581 | Not regulated | NC-NC-NC | 264843_at | At1g03400 | 2-oxoglutarate-dependent dioxygenase, putative similar to 2A6 (GI: 599622) and tomato ethylene synthesis regulatory protein E8 |
| (SP|P10967); similar to ESTs emb|Z34690, gb|T04168, gb|H37738, gb|T76913, gb|T43801, amd gb|T21964 (68414.m00320) | |||||
| 582 | Not regulated | NC-NC-NC | 264845_at | At1g03675 | |
| 583 | Not regulated | NC-NC-NC | 264789_at | At2g17870 | cold-shock DNA-binding family protein contains Pfam domains, PF00313: ‘Cold-shock’ DNA-binding domain and PF00098: Zinc |
| knuckle (68415.m02070) | |||||
| 584 | Not regulated | NC-NC-NC | 264796_at | At1g08690 | |
| 585 | Not regulated | NC-NC-NC | 264799_at | At1g08550 | violaxanthin de-epoxidase precursor, putative (AVDE1) similar to EST gb|N37612 (68414.m00948) |
| 586 | Not regulated | NC-NC-NC | 264802_at | At1g08560 | syntaxin-related protein KNOLLE (KN)/syntaxin 111 (SYP111) identical to SP|Q42374 Syntaxin-related protein KNOLLE (Syntaxin |
| 111) (AtSYP111) {Arabidopsis thaliana}; BAC F22O13 has a deletion of a cytosine at position 7887 (68414.m00949) | |||||
| 587 | Not regulated | NC-NC-NC | 264803_at | At1g08580 | expressed protein (68414.m00951) |
| 588 | Not regulated | NC-NC-NC | 264807_at | At1g08700 | presenilin family protein similar to SP|P52166 Presenilin set-12 {Caenorhabditis elegans}; contains Pfam profile PF01080: |
| Presenilin (68414.m00966) | |||||
| 589 | Not regulated | NC-NC-NC | 264808_at | At1g08770 | prenylated rab acceptor (PRA1) family protein weak similarity to prenylated Rab acceptor 1 (PRA1) [Homo sapiens] GI: 4877285; |
| contains Pfam profile PF03208: Prenylated rab acceptor (PRA1) (68414.m00976) | |||||
| 590 | Not regulated | NC-NC-NC | 264809_at | At1g08830 | superoxide dismutase [Cu—Zn] (SODCC)/copper/zinc superoxide dismutase (CSD1) identical to SWISS-PROT: P24704 |
| (68414.m00983) | |||||
| 591 | Not regulated | NC-NC-NC | 264810_at | At1g08750 | GPI-anchor transamidase, putative similar to SP|P49018 GPI-anchor transamidase (EC 3.—.—.—) (GPI transamidase) |
| {Saccharomyces cerevisiae}; contains Pfam profile PF01650: Peptidase C13 family (68414.m00972) | |||||
| 592 | Not regulated | NC-NC-NC | 264811_at | At1g08640 | expressed protein (68414.m00959) |
| 593 | Not regulated | NC-NC-NC | 264778_at | At1g08780 | prefoldin, putative similar to Swiss-Prot: Q9NQP4 prefoldin subunit 4 (Protein C-1) [Homo sapiens] (68414.m00977) |
| 594 | Not regulated | NC-NC-NC | 264782_at | At1g08810 | myb family transcription factor (MYB60) (68414.m00980) |
| 595 | Not regulated | NC-NC-NC | 264767_at | At1g61380 | S-locus protein kinase, putative similar to Ki domain interacting kinase 1 [Zea mays] gi|2735017|gb|AAB93834; contains S-locus |
| glycoprotein family domain, Pfam: PF00954 (68414.m06917) | |||||
| 596 | Not regulated | NC-NC-NC | 264772_at | At1g22930 | T-complex protein 11 contains Pfam PF05794: T-complex protein 11 (68414.m02866) |
| 597 | Not regulated | NC-NC-NC | 264724_at | At1g22920 | COP9 signalosome subunit 5B/CSN subunit 5B (CSN5B)/c-JUN coactivator protein AJH1, putative (AJH1) COP9 complex |
| subunit CSN5-1; identical to Arabidopsis homologs of a c-Jun coactivator AJH1 GI: 3641314 from [Arabidopsis thaliana]; identical to | |||||
| cDNA CSN complex subunit 5B (CSN5B) GI: 18056662; contains Pfam profile PF01398: Mov34/MPN/PAD-1 family | |||||
| (68414.m02864) | |||||
| 598 | Not regulated | NC-NC-NC | 264725_at | At1g22885 | expressed protein (68414.m02858) |
| 599 | Not regulated | NC-NC-NC | 264727_at | At1g22840 | cytochrome c, putative similar to cytochrome c [Pumpkin, Winter squash] SWISS-PROT: P00051 (68414.m02852) |
| 600 | Not regulated | NC-NC-NC | 264728_at | At1g22850 | expressed protein (68414.m02853) |
| 601 | Not regulated | NC-NC-NC | 264736_at | At1g62200 | proton-dependent oligopeptide transport (POT) family protein contains Pfam profile: PF00854 POT family; contains non- |
| consensus GA donor site at intron 4 (68414.m07016) | |||||
| 602 | Not regulated | NC-NC-NC | 264716_at | At1g70170 | matrixin family protein similar to SP|P29136 Metalloendoproteinase 1 precursor (EC 3.4.24.—) (SMEP1) {Glycine max}; contains |
| InterPro accession IPR001818: Matrixin (68414.m08074) | |||||
| 603 | Not regulated | NC-NC-NC | 264692_at | At1g70000 | DNA-binding family protein contains Pfam domains, PF00249: Myb-like DNA-binding domain and PF00098: Zinc knuckle |
| (68414.m08056) | |||||
| 604 | Not regulated | NC-NC-NC | 264699_at | At1g69980 | expressed protein (68414.m08054) |
| 605 | Not regulated | NC-NC-NC | 264700_at | At1g70100 | expressed protein (68414.m08065) |
| 606 | Not regulated | NC-NC-NC | 264701_at | At1g70160 | expressed protein similar to hypothetical protein GI: 4455225 from [Arabidopsis thaliana] (68414.m08073) |
| 607 | Not regulated | NC-NC-NC | 264703_at | At1g69960 | serine/threonine protein phosphatase PP2A-5 catalytic subunit (PP2A5) identical to SP|Q04951|P2A5_ARATH Serine/threonine |
| protein phosphatase PP2A-5 catalytic subunit (EC 3.1.3.16) {Arabidopsis thaliana}; contains Pfam profile PF00149: Ser/Thr protein | |||||
| phosphatase (68414.m08051) | |||||
| 608 | Not regulated | NC-NC-NC | 264705_at | At1g09620 | tRNA synthetase class I (I, L, M and V) family protein similar to cytosolic leucyl-tRNA synthetase [Candida albicans] GI: 9858190; |
| contains Pfam profile PF00133: tRNA synthetases class I (I, L, M and V) (68414.m01079) | |||||
| 609 | Not regulated | NC-NC-NC | 264708_at | At1g09740 | ethylene-responsive protein, putative similar to ER6 protein [Lycopersicon esculentum] GI: 5669654; contains Pfam profile |
| PF00582: universal stress protein family (68414.m01093) | |||||
| 610 | Not regulated | NC-NC-NC | 264709_at | At1g09770 | myb family transcription factor contains Pfam profile: PF00249 myb-like DNA-binding domain (68414.m01096) |
| 611 | Not regulated | NC-NC-NC | 264687_at | At1g09850 | cysteine protease, papain-like (XBCP3) identical to papain-like cysteine peptidase XBCP3 GI: 14600257 from [Arabidopsis |
| thaliana]; contains Pfam profiles PF00112: Papain family cysteine protease and PF00396: Granulin (68414.m01109) | |||||
| 612 | Not regulated | NC-NC-NC | 264664_at | At1g09660 | KH domain-containing quaking protein, putative similar to GB: AAC67357 (68414.m01084) |
| 613 | Not regulated | NC-NC-NC | 264668_at | At1g09780 | 2,3-biphosphoglycerate-independent phosphoglycerate mutase, putative/phosphoglyceromutase, putative strong similarity to |
| SP|Q42908 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1) (Phosphoglyceromutase) | |||||
| {Mesembryanthemum crystallinum}; contains Pfam profile PF01676: Metalloenzyme superfamily (68414.m01097) | |||||
| 614 | Not regulated | NC-NC-NC | 264669_at | At1g09630 | Ras-related GTP-binding protein, putative similar to GTP-binding protein GI: 1370146 from [Lotus japonicus] (68414.m01080) |
| 615 | Not regulated | NC-NC-NC | 264670_s_at | At1g09640 | elongation factor 1B-gamma, putative/eEF-1B gamma, putative Similar to elongation factor 1-gamma (gb|EF1G_XENLA). ESTs |
| gb|T20564, gb|T45940, gb|T04527 come from this gene (68414.m01081) | |||||
| 616 | Not regulated | NC-NC-NC | 264676_at | At1g09870 | histidine add phosphatase family protein contains Pfam profile PF00328: Histidine acid phosphatase; similar to multiple inositol |
| polyphosphate phosphatase (GI: 4105496)[Mus musculus]; EST gb|R64758 comes from this gene (68414.m01111) | |||||
| 617 | Not regulated | NC-NC-NC | 264679_s_at | At1g09690 | 60S ribosomal protein L21 (RPL21C) Similar to ribosomal protein L21 (gb|L38826). ESTs gb|AA395597, gb|ATTS5197 come from |
| this gene (68414.m01088) | |||||
| 618 | Not regulated | NC-NC-NC | 264685_at | At1g65610 | endo-1,4-beta-glucanase, putative/cellulase, putative similar to endo-1,4-beta-glucanase GI: 2065530 from [Lycopersicon |
| esculentum] (68414.m07442) | |||||
| 619 | Not regulated | NC-NC-NC | 264627_at | At1g65520 | enoyl-CoA hydratase/isomerase family protein low similarity to enoyl-CoA hydratase [Escherichia coli]GI: 2764828, carnitine |
| racemase SP|P31551 [Escherichia coli]; contains Pfam profile PF00378 enoyl-CoA hydratase/isomerase family protein | |||||
| (68414.m07434) | |||||
| 620 | Not regulated | NC-NC-NC | 264639_at | At1g65650 | ubiquitin carboxyl-terminal hydrolase family 1 protein similar to 26S proteasome regulatory complex subunit p37A [Drosophila |
| melanogaster] GI: 6434962; contains Pfam profile PF01088: Ubiquitin carboxyl-terminal hydrolase, family 1 (68414.m07448) | |||||
| 621 | Not regulated | NC-NC-NC | 264641_at | At1g09130 | ATP-dependent Clp protease proteolytic subunit, putative similar to nClpP5 GI: 5360595 from [Arabidopsis thaliana] |
| (68414.m01017) | |||||
| 622 | Not regulated | NC-NC-NC | 264644_at | At1g08960 | cation exchanger, putative (CAX11) similar to sodium/calcium exchanger protein [Mus musculus] gi|13925861|gb|AAK49407; |
| Ca2+:Cation Antiporter (CaCA) Family member PMID: 11500563 (68414.m00996) | |||||
| 623 | Not regulated | NC-NC-NC | 264645_at | At1g08940 | phosphoglycerate/bisphosphoglycerate mutase family protein contains Pfam profile PF00300: phosphoglycerate mutase family |
| (68414.m00995) | |||||
| 624 | Not regulated | NC-NC-NC | 264649_at | At1g09060 | transcription factor jumonji (jmjC) domain-containing protein contains Pfam domain, PF02373: jmjC domain (68414.m01010) |
| 625 | Not regulated | NC-NC-NC | 264651_at | At1g08880 | histone H2A, putative Strong similarity to histone H2A Cicer arietinum SP|O65759, Picea abies SP|P35063; contains Pfam profile |
| PF00125 Core histone H2A/H2B/H3/H4; ESTs gb|ATTS3874, gb|T46627, gb|T14194 come from this gene (68414.m00988) | |||||
| 626 | Not regulated | NC-NC-NC | 264653_at | At1g08980 | amidase family protein similar to component of chloroplast outer membrane translocon Toc64 [Pisum sativum] GI: 7453538; |
| contains Pfam profile PF01425: Amidase; supporting cDNA gi|11493701|gb|AF202077.1|AF202077 (68414.m01001) | |||||
| 627 | Not regulated | NC-NC-NC | 264656_at | At1g09010 | glycoside hydrolase family 2 protein low similarity to mannosidase [gi: 5359712] from Cellulomonas fimi (68414.m01005) |
| 628 | Not regulated | NC-NC-NC | 264657_at | At1g09100 | 26S protease regulatory subunit 6A, putative identical to SP: O04019 from [Arabidopsis thaliana] (68414.m01016) |
| 629 | Not regulated | NC-NC-NC | 264595_at | At1g04750 | synaptobrevin family protein similar to Vesicle-associated membrane protein 722 (AtVAMP722) Synaptobrevin-related protein 1 |
| (SP: P47192) {Arabidopsis thaliana}; (68414.m00470) | |||||
| 630 | Not regulated | NC-NC-NC | 284609_at | At1g04530 | expressed protein (68414.m00445) |
| 631 | Not regulated | NC-NC-NC | 264613_at | At1g04640 | biotin/lipoate A/B protein ligase family protein similar to lipoyltransferase (LIP2p) [Arabidopsis thaliana] GI: 15887052; contains Pfam |
| profile PF03099: Biotin/lipoate A/B protein ligase family (68414.m00460) | |||||
| 632 | Not regulated | NC-NC-NC | 264615_at | At1g04590 | expressed protein isofonn contains GG acceptor splice site at intron 7 (68414.m00452) |
| 633 | Not regulated | NC-NC-NC | 264619_at | At2g17760 | aspartyl protease family protein contains Eukaryotic and viral aspartyl proteases active site, PROSITE: PS00141 (68415.m02057) |
| 634 | Not regulated | NC-NC-NC | 264622_at | At2g17790 | vacuolar protein sorting-associated protein 35 family protein/VPS35 family protein similar to vacuolar protein sorting 35 [Mus |
| musculus] GI: 11875394; contains Pfam profile PF03635: Vacuolar protein sorting-associated protein 35 (68415.m02062) | |||||
| 635 | Not regulated | NC-NC-NC | 264591_at | At2g17670 | pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat (68415.m02045) |
| 636 | Not regulated | NC-NC-NC | 264594_at | At2g17640 | serine O-acetyltransferase, putative (SAT-106) similar to Arabidopsis thaliana serine acetyltransferase GI: 905391 (68415.m02040) |
| 637 | Not regulated | NC-NC-NC | 264570_at | At1g05350 | thiF family protein low similarity to SP|P30138 Adenylyltransferase thiF (EC 2.7.7.—) {Escherichia coli}; contains Pfam profile |
| PF00899: ThiF family (68414.m00542) | |||||
| 638 | Not regulated | NC-NC-NC | 264575_at | At1g05190 | ribosomal protein L6 family protein Similar to Mycobacterium RipF (gb|Z84395). ESTs gb|T75785, gb|R30580, gb|T04698 come from |
| this gene (68414.m00523) | |||||
| 639 | Not regulated | NC-NC-NC | 264576_at | At1g05360 | expressed protein Similar to Arabidopsis hypothetical protein PID: e326839 (gb|297337) contains transmembrane domains |
| (68414.m00543) | |||||
| 640 | Not regulated | NC-NC-NC | 264579_at | At1g05205 | expressed protein (68414.m00525) |
| 641 | Not regulated | NC-NC-NC | 264584_at | At1g05140 | membrane-associated zinc metalloprotease, putative similar to Hypothetical zinc metalloprotease All3971 (SP: Q8YQ64) [strain |
| PCC 7120] {Anabaena sp.}; Similar to Synechocystis hypothetical protein (gb|D80908); contains Pfam PF00595: PDZ domain (Also | |||||
| known as DHR or GLGF); contains TIGRFAM TIGR00054: membrane-associated zinc metalloprotease, putative (68414.m00517) | |||||
| 642 | Not regulated | NC-NC-NC | 264585_at | At1g05180 | auxin-resistance protein AXR1 (AXR1) identical to SP|P42744 Auxin-resistance protein AXR1 {Arabidopsis thaliana}; contains Pfam |
| profile PF00899: ThiF family; identical to cDNA ubiquitin activating enzyme E1-related protein (AXR1) GI: 304103 (68414.m00521) | |||||
| 643 | Not regulated | NC-NC-NC | 264545_at | At1g55670 | photosystem I reaction center subunit V, chloroplast, putative/PSI-G, putative (PSAG) identical to SP|Q9S7N7; similar to |
| SP|Q00327 Photosystem I reaction center subunit V, chloroplast precursor (PSI-G) (Photosystem I 9 kDa protein) {Hordeum | |||||
| vulgare}; contains Pfam profile PF01241: Photosystem I psaG/psaK (68414.m06372) | |||||
| 644 | Not regulated | NC-NC-NC | 264504_at | At1g09430 | ATP-citrate synthase (ATP-citrate (pro-S-)-lyase/citrate cleavage enzyme), putative similar to ATP-citrate-lyase (GI: 16648642) |
| [Arabidopsis thaliana]; similar to ATP-citrate (pro-S-)-lyase (EC 4.1.3.8) (Citrate cleavage enzyme)(SP: Q91V92) {Mus musculus}; | |||||
| Location of EST gb|Z34587 (68414.m01055) | |||||
| 645 | Not regulated | NC-NC-NC | 264510_at | At1g09530 | phytochrome interacting factor 3 (PIF3) identical to phytochrome interacting factor 3 (PIF3) GI: 3929585 from [Arabidopsis thaliana] |
| (68414.m01068) | |||||
| 646 | Not regulated | NC-NC-NC | 264515_at | At1g10080 | |
| 647 | Not regulated | NC-NC-NC | 264523_at | At1g10030 | integral membrane family protein contains Pfam PF03694: Erg28 like protein (68414.m01131) |
| 648 | Not regulated | NC-NC-NC | 264529_at | At1g30820 | CTP synthase, putative/UTP-ammonia ligase, putative similar to SP|P17812 CTP synthase (EC 6.3.4.2) (UTP-ammonia ligase) |
| {Homo sapiens}; contains Pfam profile PF00117: glutamine amidotransferase class-I; similar to ESTs gb|AA660762, gb|AA220982, | |||||
| dbj|AU008137, gb|AI054783, and gb|AA100804 (68414.m03768) | |||||
| 649 | Not regulated | NC-NC-NC | 264474_s_at | At1g67090 | ribulose bisphosphate carboxylase small chain 1A/RuBisCO small subunit 1A (RBCS-1A) (ATS1A) identical to SP|P10795 |
| Ribulose bisphosphate carboxylase small chain 1A, chloroplast precursor (EC 4.1.1.39) (RuBisCO small subunit 1A) {Arabidopsis | |||||
| thaliana} (68414.m07629) | |||||
| 650 | Not regulated | NC-NC-NC | 264476_at | At1g77130 | glycogenin glucosyltransferase (glycogenin)-related contains similarity to glycogenin-1 from Mus musculus [SP|Q9R062], Rattus |
| norvegicus [SP|O08730], Homo sapiens [SP|P46976] (68414.m08985) | |||||
| 651 | Not regulated | NC-NC-NC | 264484_at | At1g77260 | dehydration-responsive protein-related similar to early-responsive to dehydration stress ERD3 protein [Arabidopsis thaliana] |
| GI: 15320410; contains Pfam profile PF03141: Putative methyltransferase (68414.m08996) | |||||
| 652 | Not regulated | NC-NC-NC | 264438_at | At1g27400 | 60S ribosomal protein L17 (RPL17A) similar to GB: P51413 from [Arabidopsis thaliana]; similar to ESTs gb|L33542 and |
| gb|AA660016 (68414.m03340) | |||||
| 653 | Not regulated | NC-NC-NC | 264439_at | At1g27450 | adenine phosphoribosyltransferase 1 (APT1) nearly identical to SP|P31186 Adenine phosphoribosyltransferase 1 (EC 2.4.2.7) |
| (APRT) {Arabidopsis thaliana} (68414.m03346) | |||||
| 654 | Not regulated | NC-NC-NC | 264440_at | At1g27340 | F-box family protein contains Pfam PF00646: F-box domain; similar to fim protein; similar to ESTs gb|T42445, gb|T76780, |
| gb|AA650733, and emb|Z17748 (68414.m03330) | |||||
| 655 | Not regulated | NC-NC-NC | 264442_at | At1g27480 | lecithin:cholesterol acyltransferase family protein/LACT family protein similar to LCAT-like lysophospholipase (LLPL) [Homo |
| sapiens] GI: 4589720; contains Pfam profile PF02450: Lecithin:cholesterol acyltransferase {phosphatidylcholine-sterol | |||||
| acyltransferase} (68414.m03350) | |||||
| 656 | Not regulated | NC-NC-NC | 264443_at | At1g27385 | expressed protein (68414.m03338) |
| 657 | Not regulated | NC-NC-NC | 264446_at | At1g27310 | nuclear transport factor 2 (NTF2), putative similar to Swiss-Prot: P33331 nuclear transport factor 2 (NTF-2) (Nuclear transport factor |
| P10) [Saccharomyces cerevisiae] (68414.m03327) | |||||
| 658 | Not regulated | NC-NC-NC | 264463_at | At1g10150 | expressed protein similar to ESTs gb|T20511, gb|T45308, gb|H36493, and gb|AA651176 (68414.m01144) |
| 659 | Not regulated | NC-NC-NC | 264467_at | At1g10140 | expressed protein similar to EST gb|AA598098 (68414.m01143) |
| 660 | Not regulated | NC-NC-NC | 264468_at | At1g10310 | short-chain dehydrogenase/reduclase (SDR) family protein contains INTERPRO family IPR002198 short-chain |
| dehydrogenase/reductase (SDR) superfamily (68414.m01161) | |||||
| 661 | Not regulated | NC-NC-NC | 264407_at | At1g10180 | expressed protein (68414.m01148) |
| 662 | Not regulated | NC-NC-NC | 264421_at | At1g43170 | 60S ribosomal protein L3 (RPL3A) identical to ribosomal protein GI: 166858 from [Arabidopsis thaliana] (68414.m04974) |
| 663 | Not regulated | NC-NC-NC | 264422_at | At1g43130 | expressed protein contains Pfam domain, PF04367: Protein of unknown function (DUF502) (68414.m04968) |
| 664 | Not regulated | NC-NC-NC | 264424_at | At1g61740 | expressed protein contains Pfam profile: PF01925 domain of unknown function DUF81; identical to cDNA hypothetical protein, |
| partial GI: 4079631 (68414.m06963) | |||||
| 665 | Not regulated | NC-NC-NC | 264427_at | At1g61770 | DNAJ heat shock N-terminal domain-containing protein similar to SP|Q9UBS4 DnaJ homolog subfamily B member 11 precursor |
| Homo sapiens; contains Pfam profile PF00226 DnaJ domain (68414.m06966) | |||||
| 666 | Not regulated | NC-NC-NC | 264429_at | At1g61670 | expressed protein similar to membrane protein PTM1 precursor isolog GI: 1931644 from [Arabidopsis thaliana] (68414.m06956) |
| 667 | Not regulated | NC-NC-NC | 264399_at | At1g61780 | postsynaptic protein-related similar to postsynaptic protein CRIPT GI: 3098551 from [Rattus norvegicus] (68414.m06967) |
| 668 | Not regulated | NC-NC-NC | 264374_at | At2g25180 | two-component responsive regulator family protein/response regulator family protein contains Pfam profile: PF00072 response |
| regulator receiver domain (68415.m03011) | |||||
| 669 | Not regulated | NC-NC-NC | 264376_at | At2g25070 | protein phosphatase 2C, putative/PP2C, putative (68415.m02999) |
| 670 | Not regulated | NC-NC-NC | 284381_at | At2g25100 | ribonuclease HII family protein contains Pfam profile: PF01351 ribonuclease HII (68415.m03003) |
| 671 | Not regulated | NC-NC-NC | 264396_at | At1g12050 | fumarylacetoacetase, putative similar to fumarylacetoacetase (Fumarylacetoacetate hydrolase, Beta-diketonase, FAA)[Rattus |
| norvegicus] SWISS-PROT: P25093 (68414.m01391) | |||||
| 672 | Not regulated | NC-NC-NC | 264397_at | At1g11820 | glycosyl hydrolase family 17 protein similar to elicitor inducible chitinase Nt-SubE76 GI: 11071974 from [Nicotiana tabacum] |
| (68414.m01358) | |||||
| 673 | Not regulated | NC-NC-NC | 264373_at | At1g11890 | vesicle transport protein SEC22, putative identified as SEC22 by Raikhel, NV, et al. in Plant Physiol. 124: 1558-69 (2000); similar to |
| vesicle trafficking protein gb|U91538 from Mus musculus; ESTs gb|F15494 and gb|F14097 come from this gene (68414.m01371) | |||||
| 674 | Not regulated | NC-NC-NC | 264349_at | At1g11930 | alanine racemase family protein contains Pfam domain, PF01168: Alanine racemase, N-terminal domain (68414.m01378) |
| 675 | Not regulated | NC-NC-NC | 264350_at | At1g11870 | seryl-tRNA synthetase, putative/serine-tRNA ligase, putative similar to PIR|T03949 serine-tRNA lipase (EC 6.1.1.11) serS {Zea |
| mays}; contains Pfam profiles PF00587: tRNA synthetase class II core domain (G, H, P, S and T), PF02403: Seryl-tRNA | |||||
| synthetase N-terminal domain (68414.m01367) | |||||
| 676 | Not regulated | NC-NC-NC | 264351_at | At1g03370 | C2 domain-containing protein/GRAM domain-containing protein contains Pfam profiles PF00168: C2 domain; contains PF02893: |
| GRAM domain; similar to Chain A, Crystal Structure Of Synaptotagmin III C2aC2B Length(GI: 6980525); similar to Synaptotagmin III | |||||
| (SytIII) (Swiss-Prot: P40748) [Rattus norvegicus] (68414.m00316) | |||||
| 677 | Not regulated | NC-NC-NC | 264355_at | At1g03210 | phenazine biosynthesis PhzC/PhzF family protein contains Pfam profile: PF02567 phenazine biosynthesis-like protein |
| (68414.m00299) | |||||
| 678 | Not regulated | NC-NC-NC | 264357_at | At1g03360 | exonuclease family protein similar to Exosome complex exonuclease RRP4 (Ribosome RNA processing protein 4)(SP: P38792) |
| {Saccharomyces cerevisiae} (68414.m00315) | |||||
| 679 | Not regulated | NC-NC-NC | 264362_at | At1g03290 | expressed protein ESTs gb|H36966, gb|R65511, gb|T42324 and gb|T20569 come from this gene (68414.m00307) |
| 680 | Not regulated | NC-NC-NC | 264366_at | At1g03250 | expressed protein (68414.m00303) |
| 681 | Not regulated | NC-NC-NC | 264367_at | At1g03350 | BSD domain-containing protein contains Pfam profile PF03909: BSD domain (68414.m00314) |
| 682 | Not regulated | NC-NC-NC | 264337_at | At1g70350 | expressed protein (68414.m08093) |
| 683 | Not regulated | NC-NC-NC | 264340_at | At1g70280 | NHL repeat-containing protein contains Pfam profile PF01436: NHL repeat (68414.m08085) |
| 684 | Not regulated | NC-NC-NC | 264313_at | At1g70410 | carbonic anhydrase, putative/carbonate dehydratase, putative similar to SP|P42737 Carbonic anhydrase 2 (EC 4.2.1.1) |
| (Carbonate dehydratase 2) {Arabidopsis thaliana}; contains Pfam profile PF00484: Carbonic anhydrase (68414.m08099) | |||||
| 685 | Not regulated | NC-NC-NC | 264317_at | At1g70310 | spermidine synthase 2 (SPDSYN2)/putrescine aminopropyltransferase 2 identical to SP|O48661 Spermidine synthase 2 (EC |
| 2.5.1.16) (Putrescine aminopropyltransferase 2) (SPDSY 2) {Arabidopsis thaliana} (68414.m08089) | |||||
| 686 | Not regulated | NC-NC-NC | 264322_at | At1g04190 | tetratricopeptide repeat (TPR)-containing protein low similarity to protein antigen LmSTI1 [Leishmania major] GI: 1698880; contains |
| Pfam profile PF00515 TPR Domain; EST gb|Z47802 and gb|Z48402 come from this gene (68414.m00409) | |||||
| 687 | Not regulated | NC-NC-NC | 264326_at | At1g04230 | expressed protein (68414.m00413) |
| 688 | Not regulated | NC-NC-NC | 264330_at | At1g04120 | ABC transporter family protein Strong similarity to MRP-like ABC transporter gb|U92650 from A. thaliana and canalicular multi-drug |
| resistance protein gb|L49379 from Rattus norvegicus (68414.m00401) | |||||
| 689 | Not regulated | NC-NC-NC | 264307_at | At1g61900 | expressed protein contains similarity to glutamic acid/alanine-rich protein GI: 6707830 from [Trypanosome congolense] |
| (68414.m06983) | |||||
| 690 | Not regulated | NC-NC-NC | 264289_at | At1g61890 | MATE efflux family protein similar to ripening regulated protein DDTFR18 [Lycopersicon esculentum] GI: 12231296; contains Pfam |
| profile PF01554: Uncharacterized membrane protein family (68414.m06982) | |||||
| 691 | Not regulated | NC-NC-NC | 264300_at | At1g78670 | gamma-glutamyl hydrolase, putative/gamma-Glu-X carboxypeptidase, putative/conjugase, putative similar to gamma glutamyl |
| hydrolase GI: 1679658 SP|P93164 from [Glycine max] (68414.m09170) | |||||
| 692 | Not regulated | NC-NC-NC | 264303_s_at | At1g78870 | ubiquitin-conjugating enzyme, putative nearly identical to ubiquitin-conjugating enzyme E2 [Catharanthus roseus] GI: 5381319; |
| contains Pfam profile PF00179: Ubiquitin-conjugating enzyme (68414.m09193) | |||||
| 693 | Not regulated | NC-NC-NC | 264306_at | At1g78890 | expressed protein (68414.m09196) |
| 694 | Not regulated | NC-NC-NC | 264248_at | At1g78700 | brassinosteroid signalling positive regulator-related contains similarity to BZR1 protein [Arabidopsis thaliana] |
| gi|20270971|gb|AAM18490 (68414.m09173) | |||||
| 695 | Not regulated | NC-NC-NC | 264250_at | At1g78680 | gamma-glutamyl hydrolase (GGH1)/gamma-Glu-X carboxypeptidase/conjugase identical to SP|O65355 Gamma-glutamyl |
| hydrolase precursor (EC 3.4.19.9) (Gamma-Glu-X carboxypeptidase) (Conjugase) (GH) {Arabidopsis thaliana} (68414.m09171) | |||||
| 696 | Not regulated | NC-NC-NC | 264255_at | At1g09140 | SF2/ASF-like splicing modulator (SRP30) nearly identical to SF2/ASF-like splicing modulator Srp30 [Arabidopsis thaliana] |
| GI: 4775270 (68414.m01018) | |||||
| 697 | Not regulated | NC-NC-NC | 264256_at | At1g09270 | importin alpha-1 subunit, putative (IMPA4) similar to importin alpha-1 subunit (Karyopherin alpha-1 subunit, KAP alpha) |
| [Arabidopsis thaliana] SWISS-PROT: Q96321 (68414.m01035) | |||||
| 698 | Not regulated | NC-NC-NC | 264244_at | At1g60440 | eukaryotic pantothenate kinase family protein similar to pantothenate kinase GI: 4191500 from [Aspergillus nidulans]; contains Pfam |
| profile PF03630: Fumble (68414.m06804) | |||||
| 699 | Not regulated | NC-NC-NC | 264246_at | At1g60140 | glycosyl transferase family 20 protein/trehalose-phosphatase family protein contains Pfam profile: PF02358 trehalose- |
| phosphatase (68414.m06775) | |||||
| 700 | Not regulated | NC-NC-NC | 264219_at | At1g60420 | DC1 domain-containing protein contains Pfam domain PF03107: DC1 domain (68414.m06802) |
| 701 | Not regulated | NC-NC-NC | 264221_s_at | At1g60170 | pre-mRNA processing ribonucleoprotein binding region-containing protein similar to U4/U6 snRNP-associated 61 kDa protein |
| [Homo sapiens] GI: 18249847; contains Pfam profile PF01798: Putative snoRNA binding domain (68414.m06778) | |||||
| 702 | Not regulated | NC-NC-NC | 264229_at | At1g67480 | kelch repeat-containing F-box family protein similar to SKP1 interacting partner 6 [Arabidopsis thaliana] GI: 10716957; contains |
| Pfam profiles PF01344: Kelch motif, PF00646: F-box domain (68414.m07685) | |||||
| 703 | Not regulated | NC-NC-NC | 264233_at | At1g67430 | 60S ribosomal protein L17 (RPL17B) similar to ribosomal protein GI: 19101 from [Hordeum vulgare] (68414.m07675) |
| 704 | Not regulated | NC-NC-NC | 264236_at | At1g54680 | expressed protein (68414.m06234) |
| 705 | Not regulated | NC-NC-NC | 264239_at | At1g54770 | expressed protein (68414.m06245) |
| 706 | Not regulated | NC-NC-NC | 264189_s_at | At1g54630 | acyl carrier protein 3, chloroplast (ACP-3) nearly identical to SP|P25702 Acyl carrier protein 3, chloroplast precursor (ACP) |
| {Arabidopsis thaliana} (68414.m06230) | |||||
| 707 | Not regulated | NC-NC-NC | 264190_at | At1g54830 | CCAAT-box binding transcription factor Hap5a, putative similar to heme activated protein GI: 6289057 from (Arabidopsis thaliana) |
| GI: 14577940 CCAAT-binding protein subunit HAP5 {Hypocrea jecorina} similar to Transcription factor GB: CAA74053 GI: 2398533 | |||||
| from [Arabidopsis thaliana] similarity to transcription factor Hap5a similar to transcription factor Hap5a [Arabidopsis | |||||
| thaliana](GI: 6523090) (68414.m06251) | |||||
| 708 | Not regulated | NC-NC-NC | 264192_at | At1g54710 | expressed protein contains 3 WD-40 repeats (PF00400) (1 weak) submitForm( ); (68414.m06237) |
| 709 | Not regulated | NC-NC-NC | 264193_at | At1g54610 | protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain (68414.m06228) |
| 710 | Not regulated | NC-NC-NC | 264195_at | At1g22690 | gibberellin-responsive protein, putative similar to SP|P46688 Gibberellin-regulated protein 2 precursor {Arabidopsis thaliana}; |
| contains Pfam profile PF02704: Gibberellin regulated protein (68414.m02835) | |||||
| 711 | Not regulated | NC-NC-NC | 264205_at | At1g22790 | expressed protein identical to hypothetical protein GB: CAA72910 (68414:m02847) |
| 712 | Not regulated | NC-NC-NC | 264207_at | At1g22750 | expressed protein (68414.m02842) |
| 713 | Not regulated | NC-NC-NC | 264210_at | At1g22640 | myb family transcription factor {MYB4} similar to myb-related protein GI: 1020155 from [Arabidopsis thaliana] (68414.m02828) |
| 714 | Not regulated | NC-NC-NC | 264211_at | At1g22770 | gigantea protein (GI) identical to gigantea protein SP: Q9SQI2 from [Arabidopsis thaliana] (68414.m02845) |
| 715 | Not regulated | NC-NC-NC | 264156_at | At1g65280 | DNAJ heat shock N-terminal domain-containing protein contains Pfam profile PF00226 DnaJ domain (68414.m07402) |
| 716 | Not regulated | NC-NC-NC | 264158_at | At1g65260 | PspA/IM30 family protein contains Pfam PF04012: PspA/IM30 family profile; similar to Membrane-associated 30 kDa protein, |
| chloroplast precursor (M30) (Swiss-Prot: Q03943) [Pisum sativum]; similar to phage shock protein A (GI: 28806161) [Vibrio | |||||
| parahaemolyticus]; similar to Phage shock protein A. (Swiss-Prot: P23853) [Shigella flexneri] (68414.m07398) | |||||
| 717 | Not regulated | NC-NC-NC | 264159_at | At1g65270 | expressed protein (68414.m07399) |
| 718 | Not regulated | NC-NC-NC | 264164_at | At1g65295 | expressed protein (68414.m07404) |
| 719 | Not regulated | NC-NC-NC | 264165_at | At1g65410 | ABC transporter family protein contains similarity to toluene tolerance protein Ttg2A GI: 4336798 from [Pseudomonas putida] |
| (68414.m07421) | |||||
| 720 | Not regulated | NC-NC-NC | 264168_at | At1g02080 | transcriptional regulator-related contains Pfam PF04054: CCR4-Not complex component, Not1; contains TIGRFAM TIGR01612: |
| reticulocyte binding protein; similar to General negative regulator of transcription subunit 1 (SP: P25655) {Saccharomyces | |||||
| cerevisiae}; Location of ESTs gb|T44328 and gb|AA395265 (68414.m00130) | |||||
| 721 | Not regulated | NC-NC-NC | 264170_at | At1g02260 | transmembrane protein, putative similar to P protein (Melanocyte-specific transporter protein) (SP: Q04671) {Homo sapiens}; |
| contains 8 transmembrane domains (68414.m00165) | |||||
| 722 | Not regulated | NC-NC-NC | 264172_at | At1g02120 | GRAM domain-containing protein-related contains low similarity to PF02893: GRAM domain (68414.m00138) |
| 723 | Not regulated | NC-NC-NC | 264173_at | At1g02160 | expressed protein (68414.m00143) |
| 724 | Not regulated | NC-NC-NC | 264177_at | At1g02150 | pentatricopeptide (PPR) repeat-containing protein low similarity to DNA-binding protein [Triticum aestivum] GI: 6958202; contains |
| Pfam profile: PF01535 PPR repeat (68414.m00141) | |||||
| 725 | Not regulated | NC-NC-NC | 264178_at | At1g02170 | latex-abundant family protein (AMC1)/caspase family protein contains similarity to latex-abundant protein [Hevea brasiliensis] |
| gb: AAD13216; contains Pfam profile PF00656: ICE-like protease (caspase) p20 domain (68414.m00145) | |||||
| 726 | Not regulated | NC-NC-NC | 264152_at | At1g02140 | mago nashi family protein similar to Mago Nashi, Genbank Accession Number U03559; contains Pfam PF02792: Mago nashi |
| protein domain (68414.m00140) | |||||
| 727 | Not regulated | NC-NC-NC | 264121_at | At1g02280 | GTP-binding protein (TOC33) identical to atToc33 protein (GI: 11557973) [Arabidopsis thaliana]; Carboxyl-terminal end highly |
| similar to GTP-binding protein SP: U43377, location of EST gb|AA394770 and gb|R30089; identical to cDNA for chloroplast atToc33 | |||||
| protein GI: 11557972 (68414.m00169) | |||||
| 728 | Not regulated | NC-NC-NC | 264122_at | At1g02130 | Ras-related protein (ARA-5)/small GTP-binding protein, putative identical to Ras-related protein ARA-5 SP: P28188 from |
| [Arabidopsis thaliana] (68414.m00139) | |||||
| 729 | Not regulated | NC-NC-NC | 264123_at | At1g02270 | endonuclease/exonuclease/phosphatase family protein/calcium-binding EF hand family protein contains Pfam profiles: PF03372 |
| endonuclease/exonuclease/phosphatase family, PF00036 EF hand (68414.m00167) | |||||
| 730 | Not regulated | NC-NC-NC | 264130_at | At1g79160 | expressed protein (68414.m09230) |
| 731 | Not regulated | NC-NC-NC | 264140_at | At1g79210 | 20S proteasome alpha subunit B, putative nearly identical to SP|O23708 Proteasome subunit alpha type 2 (EC 3.4.25.1) (20S |
| proteasome alpha subunit B) {Arabidopsis thaliana} and to At1g16470 (68414.m09235) | |||||
| 732 | Not regulated | NC-NC-NC | 264120_at | At1g79340 | latex-abundant protein, putative (AMC7)/caspase family protein similar to latex-abundant protein [Hevea brasiliensis] |
| gb: AAD13216; contains Pfam domain, PF00656: ICE-like protease (caspase) p20 domain (68414.m09246) | |||||
| 733 | Not regulated | NC-NC-NC | 264092_at | At1g79040 | photosystem II 10 kDa polypeptide identical to photosystem II 10 kDa polypeptide, chloroplast [precursor] SP: P27202 from |
| [Arabidopsis thaliana]; contains Pfam profile: PF04725 photosystem II 10 kDa polypeptide PsbR (68414.m09216) | |||||
| 734 | Not regulated | NC-NC-NC | 264097_s_at | At1g79010 | NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial (TYKY) identical to SP|Q42599 NADH-ubiquinone oxidoreductase |
| 23 kDa subunit, mitochondrial precursor (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-23 KD) (CI-23 KD) (Complex I-28.5 KD) | |||||
| (CI-28.5 KD) {Arabidopsis thaliana} (68414.m09213) | |||||
| 735 | Not regulated | NC-NC-NC | 264098_at | At1g79260 | expressed protein (68414.m09240) |
| 736 | Not regulated | NC-NC-NC | 264099_at | At1g79050 | DNA repair protein recA identical to DNA repair protein recA, chloroplast [Precursor] SP: Q39199 from [Arabidopsis thaliana]; |
| contains Pfam profile: PF00154 recA bacterial DNA recombination protein (68414.m09217) | |||||
| 737 | Not regulated | NC-NC-NC | 264102_at | At1g79270 | expressed protein contains Pfam profile PF04146: YT521-B-like family (68414.m09241) |
| 738 | Not regulated | NC-NC-NC | 264084_at | At2g31240 | tetratricopeptide repeat (TPR)-containing protein contains Pfam profile PF00515: TPR Domain (68415.m03815) |
| 739 | Not regulated | NC-NC-NC | 264089_at | At2g31200 | actin-depolymerizing factor 6 (ADF6) identical to SP|Q9ZSK2 Actin-depolymerizing factor 6 (ADF-6) (AtADF6) {Arabidopsis |
| thaliana} (68415.m03810) | |||||
| 740 | Not regulated | NC-NC-NC | 264059_at | At2g31305 | expressed protein (68415.m03822) |
| 741 | Not regulated | NC-NC-NC | 264061_at | At2g27970 | cyclin-dependent kinase, putative/CDK, putative similar to Cks1 protein [Arabidopsis thaliana] gi|2274859|emb|CAA03859 |
| (68415.m03390) | |||||
| 742 | Not regulated | NC-NC-NC | 264069_at | At2g28000 | RuBisCO subunit binding-protein alpha subunit, chloroplast/60 kDa chaperonin alpha subunit/CPN-60 alpha identical to SWISS- |
| PROT: P21238-RuBisCO subunit binding-protein alpha subunit, chloroplast precursor (60 kDa chaperonin alpha subunit, CPN-60 | |||||
| alpha) [Arabidopsis thaliana] (68415.m03393) | |||||
| 743 | Not regulated | NC-NC-NC | 264070_at | At2g27960 | cyclin-dependent kinase/CDK (CKS1) identical to Cks1 protein [Arabidopsis thaliana] gi|2274859|emb|CAA03859 (68415.m03389) |
| 744 | Not regulated | NC-NC-NC | 264076_at | At2g28540 | expressed protein (68415.m03467) |
| 745 | Not regulated | NC-NC-NC | 264078_at | At2g28470 | beta-galactosidase, putative/lactase, putative similar to Beta-galactosidase precursor SP: P48980 from [Lycopersicon esculentum] |
| (68415.m03460) | |||||
| 746 | Not regulated | NC-NC-NC | 264080_at | At2g28520 | vacuolar proton ATPase, putative similar to Swiss-Prot: Q93050 vacuolar proton translocating ATPase 116 kDa subunit A isoform 1 |
| (Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit, Vacuolar proton pump subunit 1, Vacuolar adenosine | |||||
| triphosphatase subunit Ac116) [Homo sapiens] (68415.m03465) | |||||
| 747 | Not regulated | NC-NC-NC | 264056_at | At2g28510 | Dof-type zinc finger domain-containing protein similar to elicitor-responsive Dof protein ERDP GI: 6092016 from [Pisum sativum] |
| (68415.m03464) | |||||
| 748 | Not regulated | NC-NC-NC | 264028_at | At2g03680 | expressed protein Alternative splicing exists based on EST evidence (68415.m00327) |
| 749 | Not regulated | NC-NC-NC | 264037_at | At2g03750 | sulfotransferase family protein similar to similar to steroid sulfotransferase 3 [Brassica napus] GI: 3420008, steroid sulfotransferase |
| 1 [Brassica napus] GI: 3420034; contains Pfam profile PF00685: Sulfotransferase domain (68415.m00335) | |||||
| 750 | Not regulated | NC-NC-NC | 264038_at | At2g03690 | coenzyme Q biosynthesis Coq4 family protein/ubiquinone biosynthesis Coq4 family protein contains Pfam profile PF05019: |
| Coenzyme Q (ubiquinone) biosynthesis protein Coq4 (68415.m00328) | |||||
| 751 | Not regulated | NC-NC-NC | 264040_at | At2g03730 | ACT domain-containing protein (ACR5) contains Pfam ACT domain PF01842 (68415.m00333) |
| 752 | Not regulated | NC-NC-NC | 264048_at | At2g22400 | NOL1/NOP2/sun family protein contains Pfam profile PF01189: NOL1/NOP2/sun family (68415.m02656) |
| 753 | Not regulated | NC-NC-NC | 263995_at | At2g22540 | short vegetative phase protein (SVP) identical to cDNA short vegetative phase protein (SVP) GI: 10944319; (68415.m02673) |
| 754 | Not regulated | NC-NC-NC | 263998_at | At2g22510 | hydroxyproline-rich glycoprotein family protein similar to proline-rich cell wall protein [Gossypium barbadense] |
| gi|451544|gb|AAA79364; contains proline-rich extensin domains, INTERPRO: IPR002965 (68415.m02670) | |||||
| 755 | Not regulated | NC-NC-NC | 264003_at | At2g22475 | GRAM domain-containing protein/ABA-responsive protein-related similar to ABA-responsive protein [Hordeum vulgare] |
| GI: 4103635; contains Pfam profile PF02893: GRAM domain (68415.m02665) | |||||
| 756 | Not regulated | NC-NC-NC | 264008_at | At2g21120 | expressed protein (68415.m02506) |
| 757 | Not regulated | NC-NC-NC | 264012_at | At2g21080 | expressed protein (68415.m02502) |
| 758 | Not regulated | NC-NC-NC | 264018_at | At2g21170 | triosephosphate isomerase, chloroplast, putative similar to Triosephosphate isomerase, chloroplast precursor. SP|P48496 from |
| Spinacia oleracea, SP|P46225 from Secale cereale (68415.m02511) | |||||
| 759 | Not regulated | NC-NC-NC | 264019_at | At2g21130 | peptidyl-prolyl cis-trans isomerase/cyclophilin (CYP2)/rotamase identical to cyclophilin [Arabidopsis thaliana] |
| gi|2443757|gb|AAB71402: similar to peptidyl-prolyl cis-trans isomerase, PPlase (cyclophilin, cyclosporin A-binding protein) | |||||
| [Arabidopsis thaliana] SWISS-PROT: P34790 (68415.m02507) | |||||
| 760 | Not regulated | NC-NC-NC | 264020_at | At2g21160 | translocon-associated protein alpha (TRAP alpha) family protein contains Pfam profile: PF03896 translocon-associated protein |
| (TRAP), alpha subunit (68415.m02510) | |||||
| 761 | Not regulated | NC-NC-NC | 264022_at | At2g21185 | expressed protein (68415.m02513) |
| 762 | Not regulated | NC-NC-NC | 264024_at | At2g21180 | expressed protein (68415.m02512) |
| 763 | Not regulated | NC-NC-NC | 263978_at | At2g42680 | ethylene-responsive transcriptional coactivator, putative similar to ethylene-responsive transcriptional coactivator [Lycopersicon |
| esculentum] gi|5669634|gb|AAD46402 (68415.m05283) | |||||
| 764 | Not regulated | NC-NC-NC | 263980_at | At2g42770 | peroxisomal membrane 22 kDa family protein contains Mpv17/PMP22 family domain, Pfam: PF04117 (68415.m05296) |
| 765 | Not regulated | NC-NC-NC | 263986_at | At2g42790 | citrate synthase, glyoxysomal, putative strong similarity to SP|P49299 Citrate synthase, glyoxysomal precursor {Cucurbita maxima}; |
| contains Pfam profile PF00285: Citrate synthase (68415.m05298) | |||||
| 766 | Not regulated | NC-NC-NC | 263987_at | At2g42690 | lipase, putative similar to lipase [Dianthus caryophyllus] GI: 4103627; contains Pfam profile PF01764: Lipase (68415.m05285) |
| 767 | Not regulated | NC-NC-NC | 263990_at | At2g42810 | serine/threonine protein phosphatase, putative similar to SP|P53042 Serine/threonine protein phosphatase 5 (EC 3.1.3.16) (PP5) |
| (Protein phosphatase T) (PPT) {Rattus norvegicus}: contains Pfam profiles PF00149: Ser/Thr protein phosphates., PF00515: TPR | |||||
| Domain (68415.m05300) | |||||
| 768 | Not regulated | NC-NC-NC | 263938_at | At2g35900 | expressed protein (68415.m04407) |
| 769 | Not regulated | NC-NC-NC | 263939_at | At2g36070 | mitochondrial import inner membrane translocase subunit TIM44, putative contains similarity to Swiss-Prot: O35857 import inner |
| membrane translocase subunit TIM44, mitochondrial precursor [Mus musculus]; contains Pfam domian PF04280: Mitochondrial | |||||
| import inner membrane, translocase subunit TIM44 (68415.m04429) | |||||
| 770 | Not regulated | NC-NC-NC | 263949_at | At2g36060 | ubiquitin-conjugating enzyme family protein similar to DNA-binding protein CROC-1B [Homo sapiens] GI: 1066082; contains Pfam |
| profile PF00179: Ubiquitin-conjugating enzyme (68415.m04427) | |||||
| 771 | Not regulated | NC-NC-NC | 263951_at | At2g35960 | harpin-induced family protein/HIN1 family protein/harpin-responsive family protein similar to harpin-induced protein hin1 |
| (GI: 1619321) [Nicotiana tabacum] (68415.m04414) | |||||
| 772 | Not regulated | NC-NC-NC | 263954_at | At2g35840 | sucrose-phosphatase 1 (SPP1) identical to sucrose-phosphatase (SPP1) [Arabidopsis thaliana] GI: 11127757 (68415.m04400) |
| 773 | Not regulated | NC-NC-NC | 263956_at | At2g35940 | homeodomain-containing protein contains ‘Homeobox’ domain signature, Prosite: PS00027 (68415.m04411) |
| 774 | Not regulated | NC-NC-NC | 263958_at | At2g38240 | pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat (68415.m04448) |
| 775 | Not regulated | NC-NC-NC | 263928_at | At2g36330 | integral membrane protein, putative contains 4 transmembrane domains; contains Pfam PF04535: Domain of unknown function |
| (DUF588); similar to putative ethylene responsive element binding protein (GI: 22135858) [Arabidopsis thaliana] (68415.m04459) | |||||
| 776 | Not regulated | NC-NC-NC | 263929_at | At2g36310 | inosine-uridine preferring nucleoside hydrolase family protein similar to Chain A, Crystal Structure Of Nucleoside Hydrolase From |
| Leishmania Major GI: 8569431; contains Pfam profile PF01156: Inosine-uridine preferring nucleoside hydrolase (68415.m04457) | |||||
| 777 | Not regulated | NC-NC-NC | 263930_at | At2g36300 | integral membrane Yip1 family protein contains Pfam domain, PF04893: Yip1 domain (68415.m04455) |
| 778 | Not regulated | NC-NC-NC | 263904_at | At2g36380 | ABC transporter family protein related to multi drug resistance proteins and P-glycoproteins (68415.m04484) |
| 779 | Not regulated | NC-NC-NC | 263906_at | At2g36250 | chloroplast division protein FtsZ (FtsZ2-1) identical to chloroplast division protein AtFtsZ2-1 [Arabidopsis thaliana] GI: 15636809, |
| plastid division protein FtsZ [Arabidopsis thaliana] GI: 14195704 (68415.m04449) | |||||
| 780 | Not regulated | NC-NC-NC | 263920_at | At2g36410 | expressed protein contains Pfam profile PF04949: Family of unknown function (DUF662) (68415.m04469) |
| 781 | Not regulated | NC-NC-NC | 263921_at | At2g36460 | fructose-bisphosphate aldolase, putative similar to PIR|S65073 fructose-bisphosphate aldolase (EC 4.1.2.13) isoenzyme C-1, |
| cytosolic [Oryza sativa]; contains Pfam profile PF00274 Fructose-bisphosphate aldolase class-I (68415.m04475) | |||||
| 782 | Not regulated | NC-NC-NC | 263922_s_at | At2g36580 | pyruvate kinase, putative similar to pyruvate kinase, cytosolic isozyme [Glycine max] SWISS-PROT: Q42806 (68415.m04486) |
| 783 | Not regulated | NC-NC-NC | 263898_at | At2g21950 | SKP1 interacting partner 6 (SKIP6) identical to SKP1 interacting partner 6 GI: 10716957 from [Arabidopsis thaliana] |
| (68415.m02608) | |||||
| 784 | Not regulated | NC-NC-NC | 263873_at | At2g21860 | violaxanthin de-epoxidase-related contains weak similarity to violaxanthin de-epoxidase precursor gi|1438875|gb|AAC49373 |
| (68415.m02597) | |||||
| 785 | Not regulated | NC-NC-NC | 263874_at | At2g21870 | expressed protein (68415.m02598) |
| 786 | Not regulated | NC-NC-NC | 263880_at | At2g21960 | expressed protein (68415.m02609) |
| 787 | Not regulated | NC-NC-NC | 263886_at | At2g36960 | myb family transcription factor contains Pfam profile: PF00249 myb-like DNA-binding domain (68415.m04532) |
| 788 | Not regulated | NC-NC-NC | 263867_at | At2g36830 | major intrinsic family protein/MIP family protein contains Pfam profile: MIP PF00230 (68415.m04516) |
| 789 | Not regulated | NC-NC-NC | 263840_at | At2g36885 | expressed protein (68415.m04522) |
| 790 | Not regulated | NC-NC-NC | 263842_at | At2g36835 | expressed protein (68415.m04517) |
| 791 | Not regulated | NC-NC-NC | 263856_at | At2g04410 | expressed protein (68415.m00445) |
| 792 | Not regulated | NC-NC-NC | 263859_at | At2g04360 | expressed protein (68415.m00437) |
| 793 | Not regulated | NC-NC-NC | 263862_at | At2g04550 | dual specificity protein phosphatase family protein contains Pfam profile: PF00782 dual specificity phosphates, catalytic domain |
| (68415.m00461) | |||||
| 794 | Not regulated | NC-NC-NC | 263808_at | At2g04340 | expressed protein (68415.m00431) |
| 795 | Not regulated | NC-NC-NC | 263809_at | At2g04570 | GDSL-motif lipase/hydrolase family protein similar to family II lipase EXL3 (GI: 15054388), EXL1 (GI: 15054382), EXL2 |
| (GI: 15054384) [Arabidopsis thaliana] ; contains Pfam profile PF00657: Lipase/Acylhydrolase with GDSL-like motif (68415.m00465) | |||||
| 796 | Not regulated | NC-NC-NC | 263810_at | At2g04520 | eukaryotic translation initiation factor 1A, putative/eIF-1A, putative/eIF-4C, putative strong similarity to translation initiation factor |
| (eIF-1A) [Beta vulgaris] GI: 17977975; contains Pfam profile PF01176: Eukaryotic initiation factor 1A (68415.m00458) | |||||
| 797 | Not regulated | NC-NC-NC | 263811_at | At2g04350 | long-chain-fatty-acid-CoA ligase family protein/long-chain acyl-CoA synthetase family protein (LACS8) similar to LACS 4 |
| [SP|O35547] from Rattus norvegicus, LACS 4 [S9|O60488] from Homo sapiens; contains Pfam HMM hit: AMP-binding enzymes | |||||
| PF00501 (68415.m00433) | |||||
| 798 | Not regulated | NC-NC-NC | 263821_s_at | At2g09990 | 40S ribosomal protein S16 (RPS16A) Same as GB: Q42340 (68415.m01037) |
| 799 | Not regulated | NC-NC-NC | 263824_at | At2g40360 | transducin family protein/WD-40 repeat family protein contains 4 WD-40 repeats (PF00400); similar to block of proliferation |
| protein Bop1 (GI: 1679772) [Mus musculus] (68415.m04977) | |||||
| 800 | Not regulated | NC-NC-NC | 263826_at | At2g40410 | Ca(2+)-dependent nuclease, putative similar to Ca(2+)-dependent nuclease [Arabidopsis thaliana] GI: 7684292 (68415.m04983) |
| 801 | Not regulated | NC-NC-NC | 263831_at | At2g40300 | ferritin, putative similar to ferritin subunit cowpea2 precursor [Vigna unguiculata] GI: 2970654; contains Pfam profile PF00210: |
| Ferritin-like domain (68415.m04964) | |||||
| 802 | Not regulated | NC-NC-NC | 263835_at | At2g40290 | eukaryotic translation initiation factor 2 subunit 1, putative/eIF-2A, putative/eIF-2-alpha, putative similar to Swiss-Prot: P05198 |
| eukaryotic translation initiation factor 2 subunit 1 (Eukaryotic translation initiation factor 2 alpha subunit) (eIF-2-alpha) (EIF-2alpha) | |||||
| (EIF-2A) [Homo sapiens] (68415.m04960) | |||||
| 803 | Not regulated | NC-NC-NC | 263802_at | At2g40430 | expressed protein identical to Protein At2g40430 (Swiss-Prot: O22892) [Arabidopsis thaliana]; similar to Glioma tumor suppressor |
| candidate region gene 2 protein (p60) (Swiss-Prot: Q9NZM5) [Homo sapiens] (68415.m04986) | |||||
| 804 | Not regulated | NC-NC-NC | 263805_at | At2g40400 | expressed protein similar to GI: 7572912 (At3g56140)[Arabidopsis thaliana] (68415.m04981) |
| 805 | Not regulated | NC-NC-NC | 263774_at | At2g40280 | dehydration-responsive family protein similar to early-responsive to dehydration stress ERD3 protein [Arabidopsis thaliana] |
| GI: 15320410; contains Pfam profile PF03141: Putative methyltransferase (68415.m04958) | |||||
| 806 | Not regulated | NC-NC-NC | 263776_s_at | At2g46440 | cyclic nucleotide-regulated ion channel, putative (CNGC11) similar to cyclic nucleotide and calmodulin-regulated ion channel |
| (cngc3) GI: 4581201 from [Arabidopsis thaliana] (68415.m05779) | |||||
| 807 | Not regulated | NC-NC-NC | 263778_at | At2g46470 | OXA1 protein, putative similar to Oxa1 protein [Arabidopsis thaliana] GI: 10176922 (68415.m05783) |
| 808 | Not regulated | NC-NC-NC | 263785_at | At2g46390 | expressed protein (68415.m05774) |
| 809 | Not regulated | NC-NC-NC | 263786_at | At2g46370 | auxin-responsive GH3 family protein similar to auxin-responsive GH3 product [Glycine max] GI: 18591; contains Pfam profile |
| PF03321: GH3 auxin-responsive promoter (68415.m05770) | |||||
| 810 | Not regulated | NC-NC-NC | 263787_at | At2g46420 | expressed protein (68415.m05777) |
| 811 | Not regulated | NC-NC-NC | 263796_at | At2g24540 | kelch repeat-containing F-box family protein similar to SKP1 interacting partner 4 [Arabidopsis thaliana] GI: 10716953: contains |
| Pfam profiles PF01344: Kelch motif, PF00646: F-box domain (68415.m02931) | |||||
| 812 | Not regulated | NC-NC-NC | 263797_at | At2g24570 | WRKY family transcription factor identical to WRKY transcription factor 17 GI: 15991743 from [Arabidopsis thaliana] |
| (68415.m02934) | |||||
| 813 | Not regulated | NC-NC-NC | 263799_at | At2g24550 | expressed protein weak similarity to MTD1 [Medicago truncatula] GI: 9294810 (68415.m02932) |
| 814 | Not regulated | NC-NC-NC | 263750_at | At2g21530 | forkhead-associated domain-containing protein/FHA domain-containing protein (68415.m02562) |
| 815 | Not regulated | NC-NC-NC | 263756_at | At2g21270 | ubiquitin fusion degradation UFD1 family protein similar to SP|P70362 Ubiquitin fusion degradation protein 1 homolog (UB fusion |
| protein 1) {Mus musculus}; contains Pfam profile PF03152: Ubiquitin fusion degradation protein UFD1 (68415.m02532) | |||||
| 816 | Not regulated | NC-NC-NC | 263759_at | At2g21290 | expressed protein (68415.m02534) |
| 817 | Not regulated | NC-NC-NC | 263760_at | At2g21280 | expressed protein similar to YfhF (GI: 2804536) [Bacillus subtilis] (68415.m02533) |
| 818 | Not regulated | NC-NC-NC | 263761_at | At2g21330 | fructose-bisphosphate aldolase, putative strong similarity to plastidic fructose-bisphosphate aldolase (EC 4.1.2.13) from Nicotiana |
| paniculata (NPALDP1) [GI: 4827251], Oryza sativa, PIR2: T02057 [SP|Q40677] (68415.m02538) | |||||
| 819 | Not regulated | NC-NC-NC | 263764_at | At2g21410 | vacuolar proton ATPase, putative similar to vacuolar proton ATPase 100-kDa subunit from Dictyostelium discoideum |
| P|1384136|gb|AAB49621 (68415.m02548) | |||||
| 820 | Not regulated | NC-NC-NC | 263766_at | At2g21440 | RNA recognition motif (RRM)-containing protein contains InterPro entry IPR000504: RNA-binding region RNP-1 {RNA recognition |
| motif} (RRM) (68415.m02551) | |||||
| 821 | Not regulated | NC-NC-NC | 263712_at | At2g20585 | expressed protein (68415.m02405) |
| 822 | Not regulated | NC-NC-NC | 263717_at | At2g13560 | malate oxidoreductase, putative similar to NAD-dependent malic enzyme 62 kDa isoform, mitochondrial precursor (EC 1.1.1.39) |
| (NAD-ME) (SP: P37221) {Solanum tuberosum} (68415.m01495) | |||||
| 823 | Not regulated | NC-NC-NC | 263722_at | At2g13650 | GDP-mannose transporter (GONST1) identical to GDP-mannose transporter SP: Q941R4 from [Arabidopsis thaliana] |
| (68415.m01504) | |||||
| 824 | Not regulated | NC-NC-NC | 263726_at | At2g13610 | ABC transporter family protein (68415.m01500) |
| 825 | Not regulated | NC-NC-NC | 263727_at | At2g13540 | mRNA cap-binding protein (ABH1) identical to mRNA cap binding protein [Arabidopsis thaliana] GI: 15192738; contains Pfam profile |
| PF02854: MIF4G domain; identical to cDNA nuclear cap-binding protein CBP80 GI: 8515770 (68415.m01493) | |||||
| 826 | Not regulated | NC-NC-NC | 263737_at | At1g60010 | expressed protein (68414.m06761) |
| 827 | Not regulated | NC-NC-NC | 263685_at | At1g26830 | cullin, putative similar to Cutlin homolog 3 (CUL-3) SP: Q13618, GI: 3639052 from [Homo sapiens]; contains Pfam profile PF00888: |
| Cullin family (68414.m03270) | |||||
| 828 | Not regulated | NC-NC-NC | 263686_at | At1g26910 | 60S ribosomal protein L10 (RPL108) Nearly identical to ribosomal protein L10.e, Wilm's tumor suppressor homologue, gi|17682 |
| (Z15157), however differences in sequence indicate this is a different member of the L10 family (68414.m03281) | |||||
| 829 | Not regulated | NC-NC-NC | 263888_at | At1g26920 | expressed protein Location of EST 228A16T7A, gb|N65686 (68414.m03282) |
| 830 | Not regulated | NC-NC-NC | 263691_at | At1g26880 | 60S ribosomal protein L34 (RPL34A) identical to GB: Q42351, location of EST 105E2T7, gb|T22624 (68414.m03278) |
| 831 | Not regulated | NC-NC-NC | 263693_at | At1g31200 | expressed protein (68414.m03819) |
| 832 | Not regulated | NC-NC-NC | 263701_at | At1g31160 | zinc-binding protein, putative/protein kinase C inhibitor, putative similar to 14 kDa zinc-binding protein (Protein kinase C inhibitor, |
| PKCI) [Zea Mays] Swiss-Prot: P42856 (68414.m03812) | |||||
| 833 | Not regulated | NC-NC-NC | 263703_at | At1g31170 | parB-like nuclease domain-containing protein contains Pfam profile PF02195: ParB-like nuclease domain (68414.m03815) |
| 834 | Not regulated | NC-NC-NC | 263704_at | At1g31130 | expressed protein (68414.m03809) |
| 835 | Not regulated | NC-NC-NC | 263705_at | At1g31190 | inositol monophosphatase family protein similar to SP|P29218 Myo-inositol-1(or 4)-monophosphatase (EC 3.1.3.25) (Inositol |
| monophosphatase) {Homo sapiens}; contains Pfam profile PF00459: Inositol monophosphatase family; EST gb|AA597395 comes | |||||
| from this gene (68414.m03818) | |||||
| 836 | Not regulated | NC-NC-NC | 263709_at | At1g09310 | expressed protein contains Pfam profile PF04398: Protein of unknown function, DUF538 (68414.m01042) |
| 837 | Not regulated | NC-NC-NC | 263678_at | At1g04420 | aldo/keto reductase family protein Similar to SP|Q46933 Tas protein {Escherichia coli}, Babesia aldo-keto reductase SP|P40690; |
| contains Pfam profile PF00248: oxidoreductase, aldo/keto reductase family (68414.m00433) | |||||
| 838 | Not regulated | NC-NC-NC | 263651_at | At1g04340 | lesion inducing protein-related similar to ORF, able to induce HR-like lesions [Nicotiana tabacum] (68414.m00424) |
| 839 | Not regulated | NC-NC-NC | 263654_at | At1g04300 | meprin and TRAF homology domain-containing protein/MATH domain-containing protein weak similarity to ubiquitin-specific |
| protease 12 [Arabidopsis thaliana] GI: 11993471; contains Pfam PF00917; Meprin And TRAF-Homology (MATH) domain | |||||
| (68414.m00421) | |||||
| 840 | Not regulated | NC-NC-NC | 263660_at | At1g04260 | prenylated rab acceptor (PRA1) family protein weak similarity to prenylated Rab acceptor [Mus musculus] GI: 7716652; contains |
| Pfam profile PF03208: Prenylated rab acceptor (PRA1) (68414.m00417) | |||||
| 841 | Not regulated | NC-NC-NC | 263661_at | At1g04290 | thioesterase family protein contains Pfam profile PF03061: thioesterase family protein; EST gb|T45093 comes from this gene |
| (68414.m00420) | |||||
| 842 | Not regulated | NC-NC-NC | 263663_at | At1g04410 | malate dehydrogenase, cytosolic, putative strong similarity to malate dehydrogenase from Mesembryanthemum crystallinum |
| [SP|O24047], Medicago sativa [SP|O48905], Prunus persica [GI: 15982948]; contains InterPro entry IPR001236: Lactate/malate | |||||
| dehydrogenase (68414.m00432) | |||||
| 843 | Not regulated | NC-NC-NC | 263666_at | At1g04510 | transducin family protein/WD-40 repeat family protein contains 6 WO-40 repeats (PF00400); similar to cell cycle control protein |
| cwf6 (SP: O14011) [Schizosaccharomyces pombe (Fission yeast)] (68414.m00442) | |||||
| 844 | Not regulated | NC-NC-NC | 263667_at | At1g04270 | 40S ribosomal protein S15 (RPS15A) Strong similarity to Oryza 40S ribosomal protein S15. ESTs gb|R29788, gb|ATTS0365 come |
| from this gene (68414.m00418) | |||||
| 845 | Not regulated | NC-NC-NC | 263688_at | At1g04350 | 2-oxoglutarate-dependent dioxygenase, putative Similar to Arabidopsis 2A6 (gb|X83096) and to tomato ethylene synthesis |
| regulatory protein E8 (SP|P10967); EST gb|T76913 comes from this gene (68414.m00425) | |||||
| 846 | Not regulated | NC-NC-NC | 263669_at | At1g04400 | cryptochrome 2 apoprotein (CRY2)/blue light photoreceptor (PHH1) 97% identical to photolysase (PHH1) (SP: Q96524) and |
| cryptochrome 2 apoprotein (CRY2) (SP: U43397). ESTs gb|W43661 and gb|Z25638 come from this gene; contains Pfam profiles | |||||
| PF03441: FAD binding domain of DNA photolyase and PF00875: deoxyribodipyrimidine photolyase; identical to cDNA Cvi | |||||
| cryptochrome 2 (CRY2) GI: 18026275 (68414.m00430) | |||||
| 847 | Not regulated | NC-NC-NC | 263673_at | At2g04800 | expressed protein (68415.m00493) |
| 848 | Not regulated | NC-NC-NC | 263647_at | At2g04690 | cellular repressor of E1A-stimulated genes (GREG) family contains 1 transmembrane domain; similar to CREG2 (GI: 24371079) |
| [Homo sapiens] and (GI: 24371081) [Mus muscutus]; similar to cellular repressor of E1A-stimulated genes CREG (GI: 3550343) | |||||
| [Homo sapiens] (68415.m00479) | |||||
| 849 | Not regulated | NC-NC-NC | 283821_at | At2g04830 | DNA-directed RNA polymerase II, putative similar to SP|Q24320 DNA-directed RNA polymerase II 14.4 kDa polypeptide (EC |
| 2.7.7.6) (RP86) {Drosophila melanogaster}; contains PfaM profile PF01192: RNA polymerases K/14 to 18 kDa subunit | |||||
| (68415.m00473) | |||||
| 850 | Not regulated | NC-NC-NC | 263624_at | At2g04700 | ferredoxin thioredoxin reductase catalytic beta chain family protein contains Pfam profile: PF02943 ferredoxin thioredoxin |
| reductase catalytic beta (68415.m00480) | |||||
| 851 | Not regulated | NC-NC-NC | 263831_at | At2g04900 | expressed protein (68415.m00509) |
| 852 | Not regulated | NC-NC-NC | 263632_at | At2g04795 | expressed protein (68415.m00492) |
| 853 | Not regulated | NC-NC-NC | 263636_at | At2g11910 | expressed protein (68415.m01277) |
| 854 | Not regulated | NC-NC-NC | 263637_at | At2g11890 | expressed protein (68415.m01276) |
| 855 | Not regulated | NC-NC-NC | 263589_at | At2g25280 | expressed protein (68415.m03024) |
| 856 | Not regulated | NC-NC-NC | 263591_at | At2g01910 | microtubule associated protein (MAP65/ASE1) family protein low similarity to protein regulating cytokinesis 1 (PRC1) [Homo |
| sapiens] GI: 2865521; contains Pfam profile PF03999: Microtubule associated protein (MAP65/ASE1 family) (68415.m00125) | |||||
| purple acid phosphatase, putative contains Pfam profile: PF00149 calcineurin-like phosphoesterase; identical to purple acid | |||||
| 857 | Not regulated | NC-NC-NC | 263595_at | At2g01890 | phosphatase (GI: 20257479) [Arabidopsis thaliana]; (68415.m00122) |
| 858 | Not regulated | NC-NC-NC | 263557_at | At2g16400 | homeodomain-containing protein (68415.m01877) |
| 859 | Not regulated | NC-NC-NC | 263550_at | At2g17033 | pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat (68415.m01964) |
| 860 | Not regulated | NC-NC-NC | 263552_x_at | At2g24980 | proline-rich extensin-like family protein contains proline-rich extensin domains, INTERPRO: IPR002965 (68415.m02987) |
| 861 | Not regulated | NC-NC-NC | 263529_at | At2g24765 | ADP-ribosylation factor 3 (ARF3) identical to GP: 453191 ADP-ribosylation factor 3 {Arabidopsis thaliana}; contains domain |
| PF00025: ADP-ribosylation factor family (68415.m02959) | |||||
| 862 | Not regulated | NC-NC-NC | 263532_s_at | At5g37350 | RIO1 family protein similar to extragenic suppressor of the bimD6 mutation (SUDD) [Emericella nidulans] GI: 2338556, RIO1 |
| [Saccharomyces cerevisiae] GI: 1359602: contains Pfam profile PF01163: RIO1 family (68416.m04486) | |||||
| 863 | Not regulated | NC-NC-NC | 263533_at | At2g24820 | Rieske [2Fe—2S] domain-containing protein similar to Rieske iron-sulfur protein Tic55 from Pisum sativum [gi: 2764524]; contains |
| Pfam PF00355 Rieske [2Fe—2S] domain (68415.m02969) | |||||
| 864 | Not regulated | NC-NC-NC | 263537_at | At2g24790 | zinc finger (B-box type) family protein (68415.m02963) |
| 865 | Not regulated | NC-NC-NC | 263541_at | At2g24860 | chaperone protein dnaJ-related similar to Tsi1-interacting protein TSIP1 (GI: 4337001) [Nicotiana tabacum] (68415.m02973) |
| 866 | Not regulated | NC-NC-NC | 263549_at | At2g21650 | myb family transcription factor contains PFAM profile: PF00249 myb-like DNA-binding domain (68415.m02575) |
| 867 | Not regulated | NC-NC-NC | 263516_at | At2g21600 | RER1B protein identical to SP|O48671 RER1B protein (AtRER1B) {Arabidopsis thaliana} (68415.m02569) |
| 868 | Not regulated | NC-NC-NC | 263517_at | At2g21620 | universal stress protein (USP) family protein/responsive to dessication protein (RD2) strong similarity to RD2 protein [Arabidopsis |
| thaliana] GI: 15320408; contains Pfam profile PF00582: universal stress protein family; identical to cDNA RD2 GI: 15320407 | |||||
| (68415.m02571) | |||||
| 869 | Not regulated | NC-NC-NC | 263519_at | At2g21580 | 40S ribosomal protein S25 (RPS25B) (68415.m02567) |
| 870 | Not regulated | NC-NC-NC | 263491_at | At2g42600 | phosphoenolpyruvate carboxylase, putative/PEP carboxylase, putative (PPC2) strong similarity to phosphoenolpyruvate |
| carboxylase [Brassica napus] GI: 507808; contains Pfam profile PF00311: phosphoenolpyruvate carboxylase (68415.m05271) | |||||
| 871 | Not regulated | NC-NC-NC | 263493_at | At2g42520 | DEAD box RNA helicase, putative similar to SP|O00571 DEAD-box protein 3 (Helicase-like protein 2) {Homo sapiens}, DEAD box |
| RNA helicase DDX3 [Homo sapiens] GI: 3523150; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase | |||||
| conserved C-terminal domain (68415.m05262) | |||||
| 872 | Not regulated | NC-NC-NC | 263494_at | At2g42590 | 14-3-3 protein GF14 mu (GRF9) identical to GF14 mu GI: 3551052, SP: Q96299 from [Arabidopsis thaliana] (68415.m05270) |
| 873 | Not regulated | NC-NC-NC | 263495_at | At2g42530 | cold-responsive protein/cold-regulated protein (cor15b) nearly identical to cold-regulated gene cor15b [Arabidopsis thaliana] |
| GI: 456016; contains Pfam profile PF02987: Late embryogenesis abundant protein (68415.m05263) | |||||
| 874 | Not regulated | NC-NC-NC | 263496_at | At2g42570 | expressed protein (68415.m05268) |
| 875 | Not regulated | NC-NC-NC | 263513_at | At2g12400 | expressed protein (68415.m01339) |
| 876 | Not regulated | NC-NC-NC | 263488_at | At2g31840 | expressed protein (68415.m03888) |
| 877 | Not regulated | NC-NC-NC | 263459_at | At2g31820 | ankyrin repeat family protein contains ankyrin repeat domains, Pfam: PF00023 (68415.m03886) |
| 878 | Not regulated | NC-NC-NC | 263461_at | At2g31800 | ankyrin protein kinase, putative similar to ankyrin-kinase [Medicago truncatula] gi|18700701|gb|AAL78674; contains Pfam profile |
| PF00023: Ankyrin repeat identical to cDNA calcineurin B-like protein 10 (CBL10) GI: 29150247; blastp match of 67% identity and | |||||
| 1.9e−200 P-value to GP|18700701|gb|AAL78674.1|AF458699_1|AF458699 ankyrin-kinase {Medicago truncatula} (68415.m03882) | |||||
| 879 | Not regulated | NC-NC-NC | 263466_at | At2g31740 | expressed protein (68415.m03876) |
| 880 | Not regulated | NC-NC-NC | 263472_at | At2g31950 | |
| 881 | Not regulated | NC-NC-NC | 263473_at | At2g31750 | UDP-glucoronosyl/UDP-glucosyl transferase family protein contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl |
| transferase (68415.m03877) | |||||
| 882 | Not regulated | NC-NC-NC | 263482_at | At2g03980 | GDSL-motif lipase/hydrolase family protein similar to Anther-specific proline-rich protein APG from Brassica napus (SP|P40603), |
| Arabidopsis thaliana (GI: 22599); contains Pfam profile PF00857: Lipase/Acylhydrolase with GDSL-like motif (68415.m00365) | |||||
| 883 | Not regulated | NC-NC-NC | 263483_at | At2904030 | heat shock protein, putative strong similarity to heat shock protein [Arabidopsis thaliana] GI: 1906830; contains Pfam profiles |
| PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein (68415.m00371) | |||||
| 884 | Not regulated | NC-NC-NC | 263428_at | At2g22310 | ubiquitin-specific protease 4 (UBP4) identical to GI: 2347100 (68415.m02647) |
| 885 | Not regulated | NC-NC-NC | 263429_at | At2g22250 | aminotransferase class I and II family protein similar to aspartate aminotransferase from Bacillus stearothermophilus SP|Q59228, |
| Thermus aquaticus SP|O33822; contains Pfam profile PF00155 aminotransferase, classes I and II (68415.m02641) | |||||
| 886 | Not regulated | NC-NC-NC | 263442_at | At2g28605 | expressed protein (68415.m03477) |
| 887 | Not regulated | NC-NC-NC | 263424_at | At2g31510 | IBR domain-containing protein/ARIADNE-like protein ARI7 (ARI7) identical to ARIADNE-like protein ARI7 [Arabidopsis thaliana] |
| GI: 29125028; contains similarity to Swiss-Prot: Q94981 ariadne-1 protein (Ari-1) [Drosophila melanogaster]:contains Pfam profile | |||||
| PF01485: IBR domain (68415.m03850) | |||||
| 888 | Not regulated | NC-NC-NC | 263425_at | At2g31530 | secY family protein low similarity to SP|P31159 Preprotein translocase secY subunit {Synechococcus sp}; contains Pfam profile |
| PF00344: eubacterial secY protein (68415.m03852) | |||||
| 889 | Not regulated | NC-NC-NC | 263396_at | At2g31710 | expressed protein (68415.m03870) |
| 890 | Not regulated | NC-NC-NC | 263398_at | At2g31680 | Ras-related GTP-binding protein, putative similar to GTP-binding protein GI: 289370 from [Brassica napus] (68415.m03867) |
| 891 | Not regulated | NC-NC-NC | 263399_at | At2g31490 | expressed protein (68415.m03846) |
| 892 | Not regulated | NC-NC-NC | 263400_s_at | At2g31610 | 40S ribosomal protein S3 (RPS3A) (68415.m03862) |
| 893 | Not regulated | NC-NC-NC | 263406_at | At2904160 | subtilisin-like protease (AIR3) almost identical to subtilisin-like protease AIR3 GI: 4218991 from [Arabidopsis thaliana] , missing 200 |
| aa at N-terminus (68415.m00400) | |||||
| 894 | Not regulated | NC-NC-NC | 263410_at | At2g04039 | expressed protein (68415.m00383) |
| 895 | Not regulated | NC-NC-NC | 263411_at | At2g28710 | zinc finger (C2H2 type) family protein contains Pfam domain, PF00096: Zinc finger, C2H2 type (68415.m03490) |
| 896 | Not regulated | NC-NC-NC | 263413_at | At2g21240 | expressed protein (68415.m02524) |
| 897 | Not regulated | NC-NC-NC | 263414_at | At2g21230 | bZIP family transcription factor contains a bZIP transcription factor basic domain signature (PDOC00036) (68415.m02520) |
| 898 | Not regulated | NC-NC-NC | 263415_at | At2g17250 | expressed protein weak similarity to Ribosome biogenesis protein MAK21 (Swiss-Prot: Q12176) [Saccharomyces cerevisiae] |
| (68415.m01992) | |||||
| 899 | Not regulated | NC-NC-NC | 263419_at | At2g17220 | protein kinase, putative similar to protein kinase APK1A [Arabidopsis thaliana] Swiss-Prot: Q06548 (68415.m01988) |
| 900 | Not regulated | NC-NC-NC | 263420_at | At2g17240 | expressed protein (68415.m01991) |
| 901 | Not regulated | NC-NC-NC | 263422_s_at | At2g17200 | ubiquitin family protein weak similarity to PLIC-2 (ubiquitin-like type II) [Homo sapiens] GI: 9937505; contains Pfam profiles |
| PF00240: Ubiquitin family, PF00627: UBA/TS-N domain (68415.m01986) | |||||
| 902 | Not regulated | NC-NC-NC | 263371_at | At2g20490 | nucleolar RNA-binding Nop10p family protein similar to Nop10p (GI: 8096260) [Homo sapiens] (68415.m02392) |
| 903 | Not regulated | NC-NC-NC | 263334_at | At2g03820 | nonsense-mediated mRNA decay NMD3 family protein contains Pfam profile: PF04981 NMD3 family (68415.m00343) |
| 904 | Not regulated | NC-NC-NC | 263344_at | At2904940 | scramblase-related weak similarity to Phospholipid scramblase 1 (PL scrambles 1) (Ca(2)-dependent phospholipid scrambles 1) |
| (Transplantability associated protein 1) (TRA1) (NOR1) (Swiss-Prot: Q9JJ00) [Mus musculus); weak similarity to Phospholipid | |||||
| scrambles 4 (PL scrambles 4) (Ca(2)-dependent phospholipid scrambles 4) (Swiss-Prot: Q9NRO2) [Homo sapiens] | |||||
| (68415.m00516) | |||||
| 905 | Not regulated | NC-NC-NC | 263345_s_at | At2g05070 | chlorophyll A-B binding protein/LHCII type II (LHCB2.2) identical to Lhcb2 protein [Arabidopsis thaliana] GI: 4741946; contains |
| Pfam profile PF00504: Chlorophyll A-B binding protein (68415.m00529) | |||||
| 906 | Not regulated | NC-NC-NC | 263350_at | At2g13360 | serine-glyoxylate aminotransferase-related similar to serine-glyoxylate aminotransferase (GI: 21535798) [Methylobacterium |
| dichloromethanicum; contains TIGRFAM TIGR01364: phosphoserine aminotransferase; contains Pfam PF00266: | |||||
| aminotransferase, class V|(68415.m01474) | |||||
| 907 | Not regulated | NC-NC-NC | 263351_at | At2g22120 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) |
| (68415.m02826) | |||||
| 908 | Not regulated | NC-NC-NC | 263356_at | At2g22090 | UBP1 interacting protein 1a (UBA1a) nearly identical to UBP1 interacting protein 1a [Arabidopsis thaliana] GI: 19574238; contains |
| InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM); based on cDNA of partial mRNA for UBP1 | |||||
| interacting protein 1a (uba1a) GI: 19574235 (68415.m02823) | |||||
| 909 | Not regulated | NC-NC-NC | 263359_at | At2g15230 | lipase family protein similar to SP|P07098 Triacylglycerol lipase, gastric precursor (EC 3.1.1.3) {Homo sapiens}; contains Pfam |
| profile PF04083: ab-hydrolase associated lipase region (68415.m01737) | |||||
| 910 | Not regulated | NC-NC-NC | 263303_at | At2g15240 | UNC-50 family protein contains Pfam profile PF05216: UNC-50 family; contains 5 transmembrane domains; similar to inner nuclear |
| membrane RNA-binding protein unc-50 related protein (GI: 2735550) [Rattus norvegicus] (68415.m01738) | |||||
| 911 | Not regulated | NC-NC-NC | 263321_at | At2g47170 | ADP-ribosylation factor 1 (ARF1) identical to ADP-ribosylation factor ARF1({Arabidopsis thaliana} (SP: P36397) (GP: 166586) |
| 912 | Not regulated | NC-NC-NC | 263325_at | At2g04240 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger. C3HC4 type (RING finger) |
| (68415.m00412) | |||||
| 913 | Not regulated | NC-NC-NC | 263330_at | At2g15320 | leucine-rich repeat family protein contains leucine rich-repeat (LRR) domains Pfam: PF00560, INTERPRO: IPR001611 |
| (68415.m01747) | |||||
| 914 | Not regulated | NC-NC-NC | 263331_at | At2g15270 | expressed protein (68415.m01741) |
| 915 | Not regulated | NC-NC-NC | 263274_at | At2g11520 | protein kinase family protein contains protein kinase domain, Pfam: PF00069 (68415.m01242) |
| 916 | Not regulated | NC-NC-NC | 263286_at | At2g36160 | 40S ribosomal protein S14 (RPS14A) (68415.m04438) |
| 917 | Not regulated | NC-NC-NC | 263288_at | At2g36130 | peptidyl-prolyl cis-trans isomerase, putative/cyclophilin, putative/rotamase, putative contains Pfam domain, PF03160: peptidyl- |
| prolyl cis-trans isomerase, cyclophilin-type (68415.m04436) | |||||
| 918 | Not regulated | NC-NC-NC | 263296_at | At2g38800 | calmodulin-binding protein-related contains similarity to potato calmodulin-binding protein PCBP GI: 17933110 from [Solanum |
| tuberosum] (68415.m04764) | |||||
| 919 | Not regulated | NC-NC-NC | 263267_at | At2g16510 | vacuolar ATP synthase 16 kDa proteolipid subunit 5/V-ATPase 16 kDa proteolipid subunit 5 (AVAP5) identical to SP|P59227 |
| Vacuolar ATP synthase 16 kDa proteolipid subunit 1/3/5 (EC 3.6.3.14) (V-ATPase 16 kDa proteolipid subunit 1/3/5) {Arabidopsis | |||||
| thaliana} GI: 926929; contains Pfam profile PF00137: ATP synthase subunit C (68415.m01893) | |||||
| 920 | Not regulated | NC-NC-NC | 263243_at | At2g31350 | hydroxyacylglutathione hydrolase, putative/glyoxalase II, putative similar to glyoxalase II isozyme [Arabidopsis thaliana] |
| gi|2570338|gb|AAC49865 (68415.m03829) | |||||
| 921 | Not regulated | NC-NC-NC | 263253_at | At2g31370 | bZIP transcription factor (POSF21) identical to GB: Q04088 (68415.m03832) |
| 922 | Not regulated | NC-NC-NC | 263255_at | At1g10490 | expressed protein contains Pfam profile PF05127: Putative ATPase (DUF699) (68414.m01181) |
| 923 | Not regulated | NC-NC-NC | 263256_at | At1g10500 | hesB-like domain-containing protein similar to IscA (putative iron-sulfur duster assembly protein) [Azotobacter vinelandii] |
| GI: 2271523; contains Pfam profile PF01521: HesB-like domain (68414.m01182) | |||||
| 924 | Not regulated | NC-NC-NC | 263235_at | At1g10430 | serine/threonine protein phosphatase PP2A-1 catalytic subunit (PP2A1) identical to SP|Q07098 Serine/threonine protein |
| phosphatase PP2A-1 catalytic subunit (EC 3.1.3.16) {Arabidopsis thaliana}; contains Pfam profile PF00149: Ser/Thr protein | |||||
| phosphatase (68414.m01175) | |||||
| 925 | Not regulated | NC-NC-NC | 263206_at | At1g10590 | DNA-binding protein-related contains weak similarity to G-quartet DNA binding protein 3 [Tetrahymena thermophila] |
| gi|4583503|gb|AAD25098 (68414.m01194) | |||||
| 926 | Not regulated | NC-NC-NC | 263208_at | At1g10480 | zinc finger (C2H2 type) family protein contains Pfam profile: PF00096 zinc finger, C2H2 type (68414.m01180) |
| 927 | Not regulated | NC-NC-NC | 263209_at | At1g10522 | expressed protein (68414.m01185) |
| 928 | Not regulated | NC-NC-NC | 263222_at | At1930640 | protein kinase, putative contains protein kinase domain, Pfam: PF00069 (68414.m03747) |
| 929 | Not regulated | NC-NC-NC | 263224_at | At1g30580 | expressed protein (68414.m03741) |
| 930 | Not regulated | NC-NC-NC | 263226_at | At1g30690 | SEC14 cytosolic factor family protein/phosphoglyceride transfer family protein contains Pfam PF00650: CRAL/TRIO domain; |
| contains Pfam PF03765: CRAL/TRIO, N-terminus; similar to cytosolic factor (Phosphatidylinositol/phosphatidylcholine transfer | |||||
| protein) (PI/PCTP) (SP: P24280) [Saccharomyces cerevisiae]; ESTs gb|T76582, gb|N06574 and gb|Z25700 come from this gene | |||||
| (68414.m03752) | |||||
| 931 | Not regulated | NC-NC-NC | 263227_at | At1g30750 | expressed protein (68414.m03758) |
| 932 | Not regulated | NC-NC-NC | 263205_at | At1g05500 | C2 domain-containing protein similar to Ca2+-dependent lipid-binding protein (CLB1) GI: 2789434 from [Lycopersicon esculentum] |
| (68414.m00561) | |||||
| 933 | Not regulated | NC-NC-NC | 263179_at | At1g05710 | ethylene-responsive protein, putative similar to ethylene-inducible ER33 protein [Lycopersicon esculentum] |
| gi|5669656|gb|AAD46413; identical to bHLH transcription factor (bHLH-alpha gene) (68414.m00592) | |||||
| 934 | Not regulated | NC-NC-NC | 263180_at | At1g05620 | inosine-uridine preferring nucleoside hydrolase family protein similar to Chain A, Crystal Structure Of Nucleoside Hydrolase From |
| Leishmania MajorGI: 8569431; contains Pfam profile PF01156: Inosine-uridine preferring nucleoside hydrolase (68414.m00583) | |||||
| 935 | Not regulated | NC-NC-NC | 263181_at | At1g05720 | selenoprotein family protein contains Prosite PS00190: Cylochrome c family heme-binding site signature; similar to 15 kDa |
| selenoprotein (GI: 12314088) {Homo sapiens} (68414.m00596) | |||||
| 936 | Not regulated | NC-NC-NC | 263183_at | At1g05570 | callose synthase 1 (CALS1)/1,3-beta-glucan synthase 1 nearly identical to callose synthase 1 catalytic subunit [Arabidopsis |
| thaliana] GI: 13649388 (68414.m00575) | |||||
| 937 | Not regulated | NC-NC-NC | 263185_at | At1g05520 | transport protein, putative similar to Swiss-Prot: Q15436 protein transport protein Sec23A [Homo sapiens] (68414.m00585) |
| 938 | Not regulated | NC-NC-NC | 263193_at | At1g38050 | expressed protein (68414.m04479) |
| 939 | Not regulated | NC-NC-NC | 263172_at | At1g54170 | ataxin-2-related similar to SCA2 (GI: 1770390) [Homo sapiens]; similar to ataxin-2 (GI: 3005020) [Mus musculus] (68414.m06175) |
| 940 | Not regulated | NC-NC-NC | 263145_at | At1g54090 | exocyst subunit EXO70 family protein contains Pfam domain PF03081: Exo70 exocyst complex subunit (68414.m06164) |
| 941 | Not regulated | NC-NC-NC | 263152_at | At1g54060 | expressed protein similar to 6b-interacting protein 1 (NtSIP1) [Nicotiana tabacum] GI: 18149189 (68414.m06160) |
| 942 | Not regulated | NC-NC-NC | 263154_at | At1g54110 | cation exchanger, putative (CAX10) Ca2+: Cation Antiporter (CaCA) Family member PMID: 11500563 (68414.m06168) |
| 943 | Not regulated | NC-NC-NC | 263156_at | At1g54030 | GDSL-motif lipase, putative similar to myrosinase-associated proteins GI: 1769968, GI: 1769970, GI: 1216391, GI: 1216389 from |
| [Brassica napus]; contains Pfam profile PF00657: GDSL-like Lipase/Acylhydrolase (68414.m06156) | |||||
| 944 | Not regulated | NC-NC-NC | 263162_at | At1g54150 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) |
| (68414.m06173) | |||||
| 945 | Not regulated | NC-NC-NC | 263170_at | At1g03000 | AAA-type ATPase family protein contains Pfam domain, PF00004: ATPase, AAA family (‘A’TPases ‘A’ssociated with diverse |
| cellular ‘A’ctivities) (68414.m00271) | |||||
| 946 | Not regulated | NC-NC-NC | 263113_at | At1g03150 | GCN5-related N-acetyltransferase (GNAT) family protein similar to SP|P07347 N-terminal acatyttransferase complex ARD1 subunit |
| (Arrest-defective protein 1) {Saccharomyces cerevisiae}; contains Pfam profile PF00583: acetyltransferase. GNAT family | |||||
| (68414.m03292) | |||||
| 947 | Not regulated | NC-NC-NC | 263120_at | At1g78490 | cytochrome P450 family protein similar to Cytochrome P450 90A1 (SP: Q42569) [Arabidopsis thaliana] (68414.m09149) |
| 948 | Not regulated | NC-NC-NC | 263122_at | At1g78510 | solanesyl diphosphate synthase (SPS) identical to solanesyl diphosphate synthase [Arabidopsis thaliana] GI: 19911233; contains |
| Pfam profile PF00348: Polyprenyl synthetase (68414.m09151) | |||||
| 949 | Not regulated | NC-NC-NC | 263126_at | At1g78460 | SOUL heme-binding family protein weak similarity to SOUL protein [Mus musculus] GI: 4886906; contains Pfam profile PF04832: |
| SOUL heme-binding protein (68414.m09143) | |||||
| 950 | Not regulated | NC-NC-NC | 263128_at | At1g78600 | zinc finger (B-box type) family protein similar to zinc finger protein GI: 3618316 from [Oryza sativa] (68414.m09160) |
| 951 | Not regulated | NC-NC-NC | 263129_at | At1g78620 | integral membrane family protein contains Pfam domain PF01940: Integral membrane protein (68414.m09162) |
| 952 | Not regulated | NC-NC-NC | 263132_at | At1g78560 | bile acid:sodium symporter family protein low similarity to SP|Q12908 lieal sodium/bile acid cotransporter {Homo sapiens}; contains |
| Pfam profile PF01758: Sodium Bile acid Symporter family (68414.m09156) | |||||
| 953 | Not regulated | NC-NC-NC | 263136_at | At1g78580 | alpha,alpha-trehalose-phosphate synthase, UDP-forming, putative/trehalose-6-phosphate synthase, putative/UDP-glucose- |
| glucosephosphate glucosyltransferase, putative similar to trehalose-6-phosphate synthase SL-TPS/P [Selaginella lepidophylla] | |||||
| GI: 4100325; contains Pfam profiles PF00982: Glycosyltransferase family 20, PF02358: Trehalose-phosphatase (68414.m09158) | |||||
| 954 | Not regulated | NC-NC-NC | 263137_at | At1g78660 | gamma-glutamyl hydrolase, putative/gamma-Glu-X carboxypeptidase, putative/conjugase, putative similar to SP|O65355 |
| Gamma-glutamyl hydrolase precursor (EC 3.4.19.9) (Gamma-Glu-X carboxypeptidase) (Conjugase) (GH) {Arabidopsis thaliana} | |||||
| (68414.m09167) | |||||
| 955 | Not regulated | NC-NC-NC | 263111_s_at | At1g65190 | protein kinase family protein contains similarity to protein kinases (68414.m07391) |
| 956 | Not regulated | NC-NC-NC | 263096_at | At2g16060 | non-symbiotic hemoglobin 1 (HB1) (GLB1) identical to SP|O24520 Non-symbiotic hemoglobin 1 (Hb1) (ARAth GUB1) {Arabidopsis |
| thaliana) (68415.m01841) | |||||
| 957 | Not regulated | NC-NC-NC | 263097_at | At2g16070 | expressed protein (68415.m01842) |
| 958 | Not regulated | NC-NC-NC | 263074_at | At2g17560 | high mobility group protein gamma (HMGgamma)/HMG protein gamma nearly identical to HMG protein (HMGgamma) |
| [Arabidopsis thaliana] GI: 2832355 (68415.m02032) | |||||
| 959 | Not regulated | NC-NC-NC | 263076_at | At2g17520 | protein kinase family protein/Ire1 homolog-2 (IRE1-2) contains protein kinase domain, Pfam: PF00069; identical to Ire1 homolog-2 |
| [Arabidopsis thaliana] GI: 15277139, cDNA Ire1 homolog-2 GI: 15277138 (68415.m02028) | |||||
| 960 | Not regulated | NC-NC-NC | 263077_at | At2g17510 | ribonuclease II family protein similar to SP|P37202 Mitotic control protein dis3 {Schizasaccharomyces pombe}; contains Pfam |
| profile PF00773: RNB-like protein (68415.m02025) | |||||
| 961 | Not regulated | NC-NC-NC | 263047_at | At2g17630 | phosphoserine aminotransferase, putative similar to Phosphoserine aminotransferase, chloroplast precursor (PSAT) (SP: Q96255) |
| [Arabidopsis thaliana]; contains TIGRFAM TIGR01364: phosphoserine aminotransferase; contains Pfam PF00266: | |||||
| aminotransferase, class V (68415.m02039) | |||||
| 962 | Not regulated | NC-NC-NC | 263019_at | At1g23870 | glycosyl transferase family 20 protein/trehalose-phosphatase family protein contains Pfam profile: PF02358 trehalose- |
| phosphatase (68414.m03011) | |||||
| 963 | Not regulated | NC-NC-NC | 263023_at | At1g23960 | expressed protein contains Pfam profile PF04776: Protein of unknown function (DUF626) (68414.m03025) |
| 964 | Not regulated | NC-NC-NC | 263035_at | At1g23860 | splicing factor RSZp21 (RSZP21)/9G8-like SR protein (SR221) nearly identical to 9G8-like splicing factor SRZ21 [Arabidopsis |
| thaliana] GI: 3435096, RSZp21 protein [Arabidopsis thaliana] GI: 2582843 (68414.m03009) | |||||
| 965 | Not regulated | NC-NC-NC | 263036_at | At1g23890 | NHL repeat-containing protein contains Pfam profile PF01436: NHL repeat (68414.m03013) |
| 966 | Not regulated | NC-NC-NC | 263037_at | At1g23230 | expressed protein (68414.m02906) |
| 967 | Not regulated | NC-NC-NC | 263044_at | At1g23360 | UbiE/COQ5 methyltransferase family protein similar to 2-hexaprenyl-1,4-naphthoquinone methyltransferase GB: BAA25267 |
| GI: 2982680 from [Micrococcus luteus]; contains Pfam profile PF01209: methlytransferase, UbiE/COQ5 family (68414.m02921) | |||||
| 968 | Not regulated | NC-NC-NC | 263010_at | At1g23330 | expressed protein (68414.m02917) |
| 969 | Not regulated | NC-NC-NC | 263014_at | At1g23400 | expressed protein (68414.m02930) |
| 970 | Not regulated | NC-NC-NC | 263015_at | At1g23430 | |
| 971 | Not regulated | NC-NC-NC | 262985_s_at | At1g23290 | 60S ribosomal protein L27A (RPL27aB) similar to 60S RIBOSOMAL PROTEIN L27A GB: P49637 GI: 1710530 from [Arabidopsis |
| thaliana] (68414.m02913) | |||||
| 972 | Not regulated | NC-NC-NC | 263000_at | At1g54350 | ABC transporter family protein similar to hypothetical ABC transporter ATP-binding protein GI: 9955395 from [Microcystis |
| aeruginosa] (68414.m06196) | |||||
| 973 | Not regulated | NC-NC-NC | 263001_at | At1g54360 | expressed protein (68414.m06197) |
| 974 | Not regulated | NC-NC-NC | 262954_at | At1g54500 | rubredoxin family protein similar to SP|P00270 Rubredoxin (Rd) {Desulfovibrio gigas}; contains Pfam profile PF00301: Rubredoxin |
| (68414.m06216) | |||||
| 975 | Not regulated | NC-NC-NC | 262955_at | At1g54520 | expressed protein (68414.m06218) |
| 976 | Not regulated | NC-NC-NC | 262956_at | At1g54270 | eukaryotic translation initiation factor 4A-2/eIF-4A-2 similar to eukaryotic translation initiation factor 4A GI: 19696 from [Nicotiana |
| plumbeginifolia] (68414.m06187) | |||||
| 977 | Not regulated | NC-NC-NC | 262957_s_at | At1g54250 | DNA-directed RNA polymerase I, II, and III, putative similar to SP|P52434 DNA-directed RNA potymerases I, II, and III 17.1 kDa |
| polypeptide (EC 2.7.7.6) (RPB17) (RPB8) {Homo sapiens}; contains Pfam profile PF03870: RNA polymerase Rpb8 | |||||
| (68414.m06185) | |||||
| 978 | Not regulated | NC-NC-NC | 262958_at | At1g54410 | dehydrin family protein contains Pfam domain, PF00257: Dehydrin (68414.m06207) |
| 979 | Not regulated | NC-NC-NC | 262959_at | At1g54290 | eukaryotic translation initiation factor SUI1, putative similar to P|P32911 Protein translation factor SUI1 {Saccharomyces |
| cerevisiae}; contains Pfam profile PF01253: Translation initiation factor SUI1 (68414.m06189) | |||||
| 980 | Not regulated | NC-NC-NC | 262960_at | At1g54320 | LEMS (ligand-effect modulator 3) family protein/CDC50 family protein Similar to GI: 11994416; GI: 4966357; GI: 4835763; |
| GI: 9757735 from [Arabidopsis thaliana] (68414.m06193) | |||||
| 981 | Not regulated | NC-NC-NC | 262964_at | At1g54380 | spliceosome protein-related contains Pfam domain, PF04938: Survival motor neuron (SMN) interacting protein 1 (SIP1) |
| (68414.m06200) | |||||
| 982 | Not regulated | NC-NC-NC | 262966_at | At1g75760 | ER lumen protein retaining receptor family protein similar to SP|P33946 ER lumen protein retaining receptor 1 (KDEL receptor 1) |
| {Bos taurus}, contains Pfam profile PF00810: ER lumen protein retaining receptor (68414.m08799) | |||||
| 983 | Not regulated | NC-NC-NC | 262975_at | At1g75540 | zinc finger (B-box type) family protein similar to zinc finger protein GB: BAA33202 GI: 3618312 from [Oryza sativa] (68414.m08779) |
| 984 | Not regulated | NC-NC-NC | 262978_at | At1g75780 | tubulin beta-1 chain (TUB1) nearly identical to SP|P12411 Tubulin beta-1 chrain {Arabidopsis thaliana} (68414.m08801) |
| 985 | Not regulated | NC-NC-NC | 262948_at | At1g75560 | zinc knuckle (CCHC-type) family protein contains Pfam domain, PF00098: Zinc knuckle (68414.m08781) |
| 986 | Not regulated | NC-NC-NC | 262929_at | At1g65720 | expressed protein (68414.m07459) |
| 987 | Not regulated | NC-NC-NC | 262930_at | At1g65690 | harpin-induced protein-related/HIN1-related/harpin-responsive protein-related similar to hin1 homolog (GI: 13122296) |
| [Arabidopsis thaliana]; similar to hin1 (GI: 22830759) [Nicotiana tabacum]; contains 1 transmembrane domain; (68414.m07458) | |||||
| 988 | Not regulated | NC-NC-NC | 262931_at | At1g65700 | small nuclear ribonucleoprotein putative/snRNP, putative/Sm protein, putative similar to U6 snRNA-associated Sm-like protein |
| LSm8 [Homo sapiens] SWISS-PROT: O95777 (68414.m07457) | |||||
| 989 | Not regulated | NC-NC-NC | 262932_at | At1g65820 | microsomal glutathione s-transferase, putative similar to MGST3_HUMAN SP: O14880 (68414.m07469) |
| 990 | Not regulated | NC-NC-NC | 262937_at | At1g79560 | FtsH protease, putative contains similarity to chloroplast FtsH protease GI: 5804762 from [Nicotiana tabacum] (68414.m09275) |
| 991 | Not regulated | NC-NC-NC | 262944_at | At1g79550 | phosphoglycerate kinase, putative similar to SP|P41758 Phosphoglycerate kinase, chloroplast precursor (EC 2.7.2.3) |
| {Chlamydomonas reinhardtii}; contains Pfam profile PF00162: phosphoglycerate kinase (68414.m09273) | |||||
| 992 | Not regulated | NC-NC-NC | 262945_at | At1g79510 | expressed protein (68414.m09268) |
| 993 | Not regulated | NC-NC-NC | 262946_at | At1g79390 | expressed protein (68414.m09252) |
| 994 | Not regulated | NC-NC-NC | 262920_at | At1g79500 | 2-dehydro-3-deoxyphosphooctonate aldolase/phospho-2-dehydro-3-deoxyoctonate aldolase/3-deoxy-D-manno-octulosonic acid |
| 8-phosphate synthetase (KDSA) identical to Swiss-Prot: Q9AV97 2-dehydro-3-deoxyphosphooctonate aldolase (EC 4.12.16) | |||||
| (Phospho-2-dehydro-3-deoxyoctonate aldolase) (3-deoxy-D-manno-octulosonic acid 6-phosphate synthetase) (KDO-8-phosphate | |||||
| synthetase) (KDO 8-P synthase) [Arabidopsis thaliana] (68414.m09265) | |||||
| 995 | Not regulated | NC-NC-NC | 262899_at | At1g59870 | ABC transporter family protein similar to PDR5-like ABC transporter GI: 1514643 from [Spirodeta polyrhiza] (68414.m06745) |
| 996 | Not regulated | NC-NC-NC | 262906_at | At1g59760 | ATP-dependent RNA heticase, putative similar to SP|P47047 ATP-dependent RNA helicase DOB1 {Saccharomyces cerevisiae}, |
| HUA enhancer 2 [Arabidopsis thaliana] GI: 16024938; contains Pfam profiles PF00270: DEAD/DEAH box helicese. PF00271: | |||||
| Helicase conserved C-terminal domain (68414.m06729) | |||||
| 997 | Not regulated | NC-NC-NC | 262909_at | At1g59830 | serine/threonine protein phosphatase PP2A-2 catalytic subunit (PP2A2) identical to SP|Q07099 Serine/threonine protein |
| phosphatase PP2A-2 catalytic subunit (EC 3.1.3.16) {Arabidopsis thaliana}; contains Pfam profile PF00149: Ser/Thr protein | |||||
| phosphatase (68414.m06736) | |||||
| 998 | Not regulated | NC-NC-NC | 262910_at | At1g59710 | expressed protein contains Pfam profile: PF04601 protein of unknown function (DUF569 (68414: m06718) |
| 999 | Not regulated | NC-NC-NC | 262913_at | At1g59960 | aldo/keto reductase, putative similar to NADPH-dependent codeinone reductase GI: 6478210 [Papaver somniferum] , NAD(P)H |
| dependent 6′-deoxychalcone synthase [Glycine max][GI: 187213] (68414.m06754) | |||||
| 1000 | Not regulated | NC-NC-NC | 262859_at | At1g64790 | translational activator family protein similar to HsGCN1 [Homo sapiens] GI: 2282576 (68414.m07346) |
| 1001 | Not regulated | NC-NC-NC | 262860_at | At1g64810 | expressed protein contains Pfam PF05634: Arabidopsis thaliana protein of unknown function (DUF794) (68414.m07348) |
| 1002 | Not regulated | NC-NC-NC | 262875_at | At1g64970 | expressed protein (68414.m07364) |
| 1003 | Not regulated | NC-NCNC | 262876_at | At1g64750 | DSS1/SEM1 family protein contains Pfam profile PF05160: DSS1/SEM1 family (66414.m07341) |
| 1004 | Not regulated | NC-NC-NC | 262878_at | At1g64770 | expressed protein (68414.m07344) |
| 1005 | Not regulated | NC-NC-NC | 262880_at | At1g64880 | ribosomal protein S5 family protein contains similarity to 30S ribosomal protein S5 GI: 6969105 from [Campylobacter jejuni] |
| (68414.m07355) | |||||
| 1006 | Not regulated | NC-NC-NC | 262881_at | At1g64890 | integral membrane transporter family protein contains 11 transmembrane domains; contains Pfam PF03092: BT1 family; contains |
| TIGRFAMS TIGR00788: folate/biopterin transporter (68414.m07356) | |||||
| 1007 | Not regulated | NC-NC-NC | 262882_at | At1g64900 | cytoduome P450, putative similar to cytochrome p450 GI: 438240 from [Solanum melongena] (68414.m07357) |
| 1008 | Not regulated | NC-NC-NC | 262885_at | At1g64740 | tubulin alpha-1 chain (TUA1) nearly identical to SP|P11139 Tubulin alpha-1 chain {Arabidopsis thaliana} (68414.m07340) |
| 1009 | Not regulated | NC-NC-NC | 262830_at | At1g14700 | purple acid phosphatase, putative contains Pfam profile: PF00149 calcineurin-like phosphoesterase; similar to purple acid |
| phosphatase (GI: 20257479) [Arabidopsis thaliana] (68414.m01757) | |||||
| 1010 | Not regulated | NC-NC-NC | 262837_at | At1g14830 | dynamin-like protein C (DL1C) nearly identical to dynamin-like protein C [Arabidopsis thaliana] GI: 19589772 (68414.m01774) |
| 1011 | Not regulated | NC-NC-NC | 262841_at | At1g14810 | semialdehyde dehydrogenase family protein similar to SP: O31219 Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11) (ASA |
| dehydrogenase) (ASADH) {Legionella pneumophila}; contains Pfam profiles PF02774: Semialdehyde dehydrogenase dimerisation | |||||
| domain, PF01118: Semialdehyde dehydrogenase NAD binding domain (68414.m01770) | |||||
| 1012 | Not regulated | NC-NC-NC | 262842_at | At1g14720 | xyloglucan: xyloglucosyl transferase/xyloglucan endotransglycosylase/endo-xyloglucan transferase (XTR2) identical to |
| endoxyloglucan transferase [Arabidopsis thaliana] GI: 5533311 (68414.m01760) | |||||
| 1013 | Not regulated | NC-NC-NC | 262844_at | At1g14890 | invertase/pectin methylesterase inhibitor family protein similar to pectinesterase GB: X85216 GI: 732912 SP|Q43111 [Phaseolus |
| vulgaris] , SP|Q42534 from Arabidopsis thaliana; contains Pfam profile PF04043: Plant invertase/pectin methylesterase inhibitor | |||||
| (68414.m01780) | |||||
| 1014 | Not regulated | NC-NC-NC | 262845_at | At1g14740 | expressed protein (68414.m01762) |
| 1015 | Not regulated | NC-NC-NC | 262848_at | At1g14685 | expressed protein (68414.m01748) |
| 1016 | Not regulated | NC-NCNC | 262796_at | At1g20850 | cysteine endopeptidase, papain-type (XCP2) identical to papain-type cysteine endopeptidase XCP2 GI: 6708183 from [Arabidopsis |
| thaliana] (68414.m02612) | |||||
| 1017 | Not regulated | NC-NC-NC | 262797_at | At1g20840 | transporter-related low similarity to D-xylose proton-symporter [Lactobacillus brevis] GI: 2895856; contains Pfam profile PF00083: |
| major facilitator superfamily protein (68414.m02611) | |||||
| 1018 | Not regulated | NC-NC-NC | 262801_at | At1g21010 | expressed protein (68414.m02829) |
| 1019 | Not regulated | NC-NC-NC | 262805_at | At1g20900 | DNA-binding protein-related contains Pfam domain PF03479: Domain of unknown function (DUF296), found in AT-hook motifs |
| Pfam: PF02178 (68414.m02617) | |||||
| 1020 | Not regulated | NC-NC-NC | 262813_at | At1g11670 | MATE efflux family protein similar to ripening regulated protein DDTFR18 [Lycopersicon esculentum] GI: 12231296; contains Pfam |
| profile PF01554: Uncharacterized membrane protein family; EST gb|W43487 comes from this gene (68414.m01340) | |||||
| 1021 | Not regulated | NC-NC-NC | 262814_at | At1g11660 | heat shock protein, putative strong similarity to gb|Z70314 heat-shock protein from Arabidopsis thaliana and is a member of the |
| PF|00012 Hsp70 protein family (68414.m01339) | |||||
| 1022 | Not regulated | NC-NC-NC | 262823_at | At1g11750 | ATP-dependent Clp protease proteolytic subunit (ClpP) identical to ATP-dependent Clp protease proteolytic subunit GI: 2827888 |
| from [Arabidopsis thaliana]; contains Pfam profile PF00574: Clp protease; contains TIGRfam profile TIGR00493: ATP-dependent | |||||
| Clp protease, proteolytic subunit ClpP (68414.m01348) | |||||
| 1023 | Not regulated | NC-NC-NC | 262826_at | At1g13080 | cytothome P450 family protein identical to gb|O78605 cytochrome P450 monooxygenase from Arabidopsis thaliana and is a |
| member of the PF|00067 Cytochrome P450 family. ESTs 91|Z18072, gb|Z35218 and gb|T43466 come from this gene | |||||
| (68414.m01516) | |||||
| 1024 | Not regulated | NC-NC-NC | 262780_at | At1g13090 | cytochrome P450 71B28, putative (CYP71B28) Identical to Cytochrome P450 (SP: Q9SAE3) [Arabidopsis thaliana]; strong similarity |
| to gb|X97864 cytochrome P450 from Arabidopsis thaliana and is a member of the PF|00067 Cytochrome P450 family. ESTs | |||||
| gb|N65665, gb|T14112, gb|T76255, gb|T20906 and gb|AI100027 come from this gene (68414.m01518) | |||||
| 1025 | Not regulated | NC-NC-NC | 262781_s_at | At1g13060 | 20S proteasome beta subunit E1 (PBE1) (PRCE) identical to GB: O23717; identical to cDNA proteasome subunit prce GI: 2511595 |
| (68414.m01514) | |||||
| 1026 | Not regulated | NC-NC-NC | 262782_at | At1g13195 | zinc finger (C3HC4-type RING finger) family protein similar to MTD2 [Medicago truncatula] GI: 9294812; contains Pfam profile |
| PF00097: Zinc finger, C3HC4 type (RING finger) (68414.m01530) | |||||
| 1027 | Not regulated | NC-NC-NC | 262786_at | At1g10740 | expressed protein (68414.m01225) |
| 1028 | Not regulated | NC-NC-NC | 262790_at | At1g10840 | eukaryotic translation initiation factor 3 subunit 3/eIF-3 gamma/eIF3h (TIF3H1) identical to SP|Q9C5Z2 Eukaryotic translation |
| initiation factor 3 subunit 3 (eIF-3 gamma) (eIF3 p38 subunit) (eIF3h) {Arabidopsis thaliana}; contains Pfam profile PF01398: | |||||
| Mov34/MPN/PAD-1 family (68414.m01245) | |||||
| 1029 | Not regulated | NC-NC-NC | 262791_at | At1g10830 | sodium symporter-related contains five transmembrane domains; Interpro IPR001991 Sodium:dicarboxylate symporter, EST |
| gb|F13926 comes from this gene (68414.m01244) | |||||
| 1030 | Not regulated | NC-NC-NC | 262736_at | At1g28570 | GDSL-motif lipase, putative similar to lipase [Arabidopsis thaliana] GI: 1145627; contains InterPro Entry IPR001087 Lipolytic |
| enzyme, G-D-S-L family (68414.m03517) | |||||
| 1031 | Not regulated | NC-NC-NC | 262745_at | At1g28600 | lipase, putative similar to lipase [Arabidopsis thaliana] GI: 1145627; contains InterPro Entry IPR001087 Lipolytic enzyme, G-D-S-L |
| family (68414.m03522) | |||||
| 1032 | Not regulated | NC-NC-NC | 262749_at | At1g28580 | GDSL-motif lipase, putative similar to lipase [Arabidopsis thaliana] GI: 1145627; contains InterPro Entry IPR001087 Lipolytic |
| enzyme, G-D-S-L family (68414.m03519) | |||||
| 1033 | Not regulated | NC-NC-NC | 262750_at | At1g28710 | expressed protein similar to GI: 2827651, GI: 7527728, GI: 4406788, GI: 6063544. GI: 10764853 from [Arabidopsis thaliana] |
| (68414.m03536) | |||||
| 1034 | Not regulated | NC-NC-NC | 262754_at | At1g16350 | inosine-5′-monophosphate dehydrogenase, putative strong similarity to SP|P47996 gb|L34684 inosine monophosphate |
| dehydrogenase (IMPDH) from Arabidopsis thaliana member of the PF|00478 IMP dehydrogenase family (68414.m01956) | |||||
| 1035 | Not regulated | NC-NC-NC | 262705_at | At1g16260 | protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain (68414.m01947) |
| 1036 | Not regulated | NC-NC-NC | 262706_at | At1g16280 | DEAD/DEAH box helicase, putative similar to gb|L13612 DEAD-box protein (dbp45A) from Drosophila melanogaster and is a |
| member of PF|00270 DEAD/DEAH box helicase family (68414.m01949) | |||||
| 1037 | Not regulated | NC-NC-NC | 262707_at | At1g16290 | expressed protein (68414.m01950) |
| 1038 | Not regulated | NC-NC-NC | 262708_at | At1g16190 | DNA repair protein RAD23, putative similar to DNA repair by nucleotide excision (NER) RAD23 protein, isoform II GI: 1914685 from |
| [Daucus carota] (68414.m01939) | |||||
| 1039 | Not regulated | NC-NC-NC | 262710_at | At1g16210 | expressed protein ESTs gb|T04357 and gb|AA595092 come from this gene (68414.m01941) |
| 1040 | Not regulated | NC-NC-NC | 262712_at | At1g16460 | mercaptopyruvate sulfurtransferase (MST2) (RDH2) identical to mercaptopyruvate sulfurtransferase GI: 6009983 and thiosulfate |
| sulfurtransferase GI: 5817004 from [Arabidopsis thaliana]; contains PF|00581 Rhodanese-like domain (68414.m01968) | |||||
| 1041 | Not regulated | NC-NC-NC | 262721_at | At1g43560 | thioredoxin family protein contains Pfam profile: PF00085 Thioredoxin; similar to thioredoxin GI: 142153 from [Synechococcus |
| PCC6301] (68414.m05000) | |||||
| 1042 | Not regulated | NC-NC-NC | 262725_at | At1g43580 | expressed protein (68414.m05003) |
| 1043 | Not regulated | NC-NC-NC | 262727_at | At1g75800 | pathogenesis-related thaumatin family protein similar to receptor serine/threonine kinase PR5K [Arabidopsis thaliana] GI: 1235680; |
| contains Pfam profile: PF00314 Thaumatin family (68414.m08805) | |||||
| 1044 | Not regulated | NC-NC-NC | 262700_at | At1g76020 | expressed protein (68414.m08826) |
| 1045 | Not regulated | NC-NC-NC | 262676_at | At1g75950 | E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 (At1) E3 ubiquitin ligase; skp1a; identical to Skp1a GI: 3068807, Skp1p |
| GI: 1432083 and UIP1 GI: 3719209 from [Arabidopsis thaliana]; contains Pfam profiles PF01466: Skp1 family, dimensation domain | |||||
| and PF03931: Skp1 family, tetramerisation domain; (68414.m08821) | |||||
| 1046 | Not regulated | NC-NC-NC | 262678_at | At1g75810 | expressed protein (68414.m08806) |
| 1047 | Not regulated | NC-NC-NC | 262682_at | At1g75900 | family II extracellular lipase 3 (EXL3) EXL3 (PMID: 11431566); similar to anter-specific proline-rich protein (APG) SP: P40602 |
| [Arabidopsis thaliana] (68414.m08816) | |||||
| 1048 | Not regulated | NC-NC-NC | 262684_s_at | At1g76030 | vacuolar ATP synthase subunit B/V-ATPase B subunit/vacuolar proton pump B subunit/V-ATPase 57 kDa subunit identical to |
| SP|P11574 Vacuolar ATP synthase subunit B (EC 3.6.3.14) (V-ATPase B subunit) (Vacuolar proton pump B subunit) (V-ATPase | |||||
| 57 kDa subunit) {Arabidopsis thaliana} (68414.m08827) | |||||
| 1049 | Not regulated | NC-NC-NC | 262686_at | At1g75990 | 26S proteasome regulatory subunit S3, putative (RPN3) similar to 26S proteasome regulatory subunit S3 SP: P93768 [Nicotiana |
| tabacum (Common tobacco)] (68414.m08824) | |||||
| 1050 | Not regulated | NC-NC-NC | 262691_at | At1g62740 | stress-inducible protein, putative similar to sti (stress inducible protein) [Glycine max] GI: 872116; contains Pfam profile PF00515 |
| TPR Domain (68414.m07081) | |||||
| 1051 | Not regulated | NC-NC-NC | 262693_at | At1g62780 | expressed protein (68414.m07086) |
| 1052 | Not regulated | NC-NC-NC | 262667_at | At1g62810 | copper amine oxidase, putative similar to copper amine oxidase [Cicer arietinum] gi|3819099|emb|CAA08855 (68414.m07091) |
| 1053 | Not regulated | NC-NC-NC | 262639_at | At1g62820 | calmodulin, putative similar to calmodulin SP: P04465 from [Trypanosoma brucei gambiense]; contains INTERPRO: IPR002048 |
| calcium-binding EF-hand domain (68414.m07092) | |||||
| 1054 | Not regulated | NC-NC-NC | 262645_at | At1g62750 | elongation factor Tu family protein similar to elongation factor G SP: P34811 [Glycine max (Soybean)] (68414.m07082) |
| 1055 | Not regulated | NC-NC-NC | 262660_at | At1g14000 | protein kinase family protein/ankyrin repeat family protein contains Pfam profiles: PF00069 protein kinase domain, PF00023 |
| ankyrin repeat (68414: m01652) | |||||
| 1056 | Not regulated | NC-NC-NC | 262607_at | At1g13990 | expressed protein (68414.m01650) |
| 1057 | Not regulated | NC-NC-NC | 262609_at | At1g13930 | expressed protein weakly similar to drought-induced protein SDi-6 (PIR: S71562) common sunflower (fragment) (68414.m01635) |
| 1058 | Not regulated | NC-NC-NC | 262612_at | At1g14150 | oxygen evolving enhancer 3 (PsbQ) family protein extrinsic pll protein; contains Pfam profile PF05757: Oxygen evolving enhancer |
| protein 3 (PsbQ) (68414.m01672) | |||||
| 1059 | Not regulated | NC-NC-NC | 262618_at | At1g06560 | NOL1/NOP2/sun family protein contains Pfam profile PF01189: NOL1/NOP2/sun family (68414.m00695) |
| 1060 | Not regulated | NC-NC-NC | 262621_at | At1g06530 | myosin heavy chain-related similar to myosin heavy chain (GI: 1408194) {Placopecten magellanicus}; similar to Myosin heavy chain, |
| clone 203 (Fragment) (SP: P39922){Hydra attenuata}; contains one transmembrane domain (68414.m00692) | |||||
| 1061 | Not regulated | NC-NC-NC | 262624_at | At1g06450 | CCR4-NOT transcription complex protein, putative similar to SWISS-PROT: Q9UFF9 CCR4-NOT transcription complex, subunit 8 |
| (CAF1-like protein, CALIFp) [Homo sapiens] (68414.m00683) | |||||
| 1062 | Not regulated | NC-NC-NC | 262626_at | At1g06430 | FtsH protease, putative similar to zinc dependent protease GI: 7650138 from [Arabidopsis thaliana] (68414: m00680) |
| 1063 | Not regulated | NC-NC-NC | 262629_at | At1g06460 | 31.2 kDa small heat shock family protein/hsp20 family protein contains Pfam profile: PF00011 Hsp20/alpha crystallin family |
| (68414.m00684) | |||||
| 1064 | Not regulated | NC-NC-NC | 262632_at | At1g06680 | photosystem II oxygen-evolving complex 23 (OEC23) JBC 14: 211-238 (2002); identical to 23 kDa polypeptide of oxygen-evolving |
| comlex (OEC) GB: CAA66785 GI: 1769905 [Arabidopsis thaliana] (68414.m00708) | |||||
| 1065 | Not regulated | NC-NC-NC | 262634_at | At1g06690 | aldo/keto reductase family protein contains Pfam profile PF00248: oxidoreductase, aldo/keto reductase family (68414.m00710) |
| 1066 | Not regulated | NC-NC-NC | 262638_at | At1g06650 | 2-oxoglutarate-dependent dioxygenase, putative similar to 2A6 (GI: 599622) and tomato ethylene synthesis regulatory protein E8 |
| (SP|P10967); contains Pfam profile: PF00671 Iron/Ascorbate oxidoreductase family (68414.m00704) | |||||
| 1067 | Not regulated | NC-NC-NC | 262581_at | At1g15370 | expressed protein (68414.m01840) |
| 1068 | Not regulated | NC-NC-NC | 262583_at | At1g15110 | phosphatidyl serine synthase family protein contains Pfam profile: PF03034 phosphatidyl serine synthase (68414.m01804) |
| 1069 | Not regulated | NC-NC-NC | 262584_at | At1g15440 | transducin family protein/WD-40 repeat family protein Strong similarity to gb X95263 Periodic tryptophan protein 2 gene (PWP2) |
| from Homo sapiens and contains 6 WD40, G-beta repeat domains (68414.m01855) | |||||
| 1070 | Not regulated | NC-NC-NC | 262590_at | At1g15100 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) |
| (68414.m01803) | |||||
| 1071 | Not regulated | NC-NC-NC | 262592_at | At1g15400 | expressed protein ESTs gb|H37295 and gb|R64895 come from this gene (68414.m01844) |
| 1072 | Not regulated | NC-NC-NC | 262593_at | At1g15120 | ubiquinol-cytochrome C reductase complex 7.8 kDa protein, putative/mitochondrial hinge protein, putative similar to SP|P48504 |
| Ubiquinol-cytochrome C reductase complex 7.8 kDa protein (EC 1.10.2.2) (Mitochondrial hinge protein) (CR7) {Solanum | |||||
| tuberosum}; contains Pfam profile PF02320: Ubiquinol-cytochrome C reductase hinge protein (68414.m01805) | |||||
| 1073 | Not regulated | NC-NC-NC | 262594_at | At1g15250 | 60S ribosomal protein L37 (RPL37A) almost identical to GB: Q43292 (68414.m01825) |
| 1074 | Not regulated | NC-NC-NC | 262598_at | At1g15260 | expressed protein EST gb|N65467 comes from this gene (68414.m01826) |
| 1075 | Not regulated | NC-NC-NC | 262599_at | At1g15350 | expressed protein (68414.m01836) |
| 1076 | Not regulated | NC-NC-NC | 262600_at | At1g15340 | methyl-CpG-binding domain-containing protein contains Pfam profile PF01429: Methyl-CpG binding domain (68414.m01835) |
| 1077 | Not regulated | NC-NC-NC | 262602_at | At1g15270 | expressed protein ESTs gb|AA650895, gb|AA720043 and gb|R29777 come from this gene (68414.m01827) |
| 1078 | Not regulated | NC-NC-NC | 262572_at | At1g15140 | oxidoreductase NAD-binding domain-containing protein Contains Pfam profile PF00175: Oxidoreductase NAD-binding domain; |
| ESTs gb|H76345 and gb|AA651465 come from this gene (68414.m01808) | |||||
| 1079 | Not regulated | NC-NC-NC | 262573_at | At1g15390 | peptide deformylase, mitochondrial/polypeptide deformylase 1A (PDF1A) nearly identical to SP|Q9FV53 Peptide deformylase, |
| mitochondrial precursor (EC 3.5.1.88) (PDF) (Polypeptide deformylase) {Arabidopsis thaliana}; contains Pfam profile PF01327: | |||||
| polypeptide deformylase; supporting cDNA gi|11320951|gb|AF250959.1|AF250959 (68414.m01843) | |||||
| 1080 | Not regulated | NC-NC-NC | 262574_at | At1g15230 | expressed protein ESTs gb|R30529, gb|Z48463, gb|Z48467, gb|AA597369 and gb|AA394772 come from this gene |
| (68414.m01823) | |||||
| 1081 | Not regulated | NC-NC-NC | 262544_at | At1g15425 | |
| 1082 | Not regulated | NC-NC-NC | 262551_at | At1g31340 | ubiquitin family protein contains INTERPRO: IPR000626 ubiquitin domain (68414.m03835) |
| 1083 | Not regulated | NC-NC-NC | 262557_at | At1g31330 | photosystem I reaction center subunit III family protein contains Pfam profile: PF02507: photosystem I reaction center subunit III |
| (68414.m03833) | |||||
| 1084 | Not regulated | NC-NC-NC | 262562_at | At1g34220 | expressed protein contains Pfam profile: PF03398 eukaryotic protein of unknown function, DUF292 (68414.m04246) |
| 1085 | Not regulated | NC-NC-NC | 262563_at | At1g34210 | somatic embryogenesis receptor-like kinase 2 (SERK2) nearly identical to somatic embryogenesis receptor-like kinase 2 |
| [Arabidopsis thaliana] GI: 14573457; contains Pfam domains PF00560: Leucine Rich Repeat and PF00069: Protein kinase domain; | |||||
| identical to cDNA somatic embryogenesis receptor-like kinase 2 (SERK2) GI: 14573456 (68414.m04245) | |||||
| 1086 | Not regulated | NC-NC-NC | 262566_at | At1g34310 | transcriptional factor B3 family protein/auxin-responsive factor AUXAAA-related contains Pfam profile: PF02309 AUX/IAA family |
| (68414.m04257) | |||||
| 1087 | Not regulated | NC-NC-NC | 262567_at | At1g34300 | lectin protein kinase family protein contains Pfam domains, PF01453: Lectin (probable mannose binding) and PF00069: Protein |
| kinase domain (68414.m04256) | |||||
| 1088 | Not regulated | NC-NC-NC | 262514_at | At1g34190 | no apical meristem (NAM) family protein contains Pfam PF02365: No apical meristem (NAM) protein; similar to NAM protein |
| GI: 6066595 [Petunia hybrida]; nam-like protein 9 (GI: 21105746) [Petunia x hybrida]; NAC1 GI: 7716952 [Medicago truncatula] | |||||
| (68414.m04241) | |||||
| 1089 | Not regulated | NC-NC-NC | 262519_at | At1g17160 | plkB-type carbohydrate kinase family protein contains Pfam profile: PF00294 pfkB family carbohydrate kinase (68414.m02092) |
| 1090 | Not regulated | NC-NC-NC | 262521_at | At1g17130 | cell cycle control protein-related contains similarity to Swiss-Prot: Q9P7C5 cell cycle control protein cwf16 [Schizosaccharomyces |
| pombe] (68414.m02087) | |||||
| 1091 | Not regulated | NC-NC-NC | 262523_at | At1g17110 | ubiquitin-specific protease 15 (UBP15) almost identical to ubiquitin-specific protease 15 GI: 11993475 [Arabidopsis thaliana], 7 |
| amino acid difference (68414.m02085) | |||||
| 1092 | Not regulated | NC-NC-NC | 262524_at | At1g17070 | D111/G-patch domain-containing protein Similar to SP|Q9ERA6 Tuftelin-interacting protein 11 {Mus musculus}; contains Pfam |
| profile PF01585: G-patch domain (68414.m02077) | |||||
| 1093 | Not regulated | NC-NC-NC | 262526_at | At1g17050 | geranyl diphosphate synthase, putative/GPPS. putative/dimethylallyltransferase, putative/prenyl transferase, putative similar to |
| GI: 11322965; Except for first 55 amino acids, 52% identical to Prenyl transferase (Cyanophora paradoxa) (gi 99282). Location of | |||||
| est 120E9T7 (gb|T43950) (68414.m02073) | |||||
| 1094 | Not regulated | NC-NC-NC | 262532_at | At1g17210 | expressed protein distantly related to dentin phosphoryn [Homo sapiens] (GI: 4322670) (68414.m02097) |
| 1095 | Not regulated | NC-NC-NC | 262537_s_at | At1g17280 | ubiquitin-conjugating enzyme, putative similar to ubiquitin conjugating enzyme 6 from [Homo sapiens] GI: 14029267, [Mus |
| musculus] GI: 14029263; contains Pfam profile PF00179: Ubiquitin-conjugating enzyme (68414.m02105) | |||||
| 1096 | Not regulated | NC-NC-NC | 262539_at | At1g17200 | integral membrane family protein Location of est 136A23T7 (gb|T45563); contains TIGRFAM TIGR01569: plant integral membrane |
| protein TIGR01569; contains Pfam PF04535: Domain of unknown function (DUF588) (68414.m02096) | |||||
| 1097 | Not regulated | NC-NC-NC | 262482_at | At1g17020 | oxidoreductase, 2OG-Fe(II) oxygenase family protein similar to flavonol synthase [Petunia x hybrida][GI: 311658], |
| leucoanthocyanidin dioxygenase [Malus domestica][SP|P51091]; contains PF03171 2OG-Fe(II) oxygenase superfamily domain | |||||
| (68414.m02067) | |||||
| 1098 | Not regulated | NC-NC-NC | 262487_at | At1g21610 | wound-responsive family protein similar to wound-responsive protein 14.05 (GI: 16506638) [Castanea sativa]; ESTs gb T42839 and |
| gb|AA395192 come from this gene (68414.m02702) | |||||
| 1099 | Not regulated | NC-NC-NC | 262488_at | At1g21820 | |
| 1100 | Not regulated | NC-NC-NC | 262489_at | At1g21830 | expressed protein EST gb|T21171 comes from this gene (68414.m02731) |
| 1101 | Not regulated | NC-NC-NC | 262497_at | At1g21720 | 20S proteasome beta subunit C1 (PBC1).(PRCT) almost identical to GB: AAC32069 from [Arabidopsis thaliana], EST gb|T76747 |
| comes from this gene; identical to cDNA proteasome subunit prct GI: 2511567 (68414.m02719) | |||||
| 1102 | Not regulated | NC-NC-NC | 262499_at | At1g21770 | expressed protein (68414.m02725) |
| 1103 | Not regulated | NC-NC-NC | 262500_at | At1g21760 | F-box family protein Contains PF|00646 F-box domain. ESTs gb|Z37267, gb|R90412, gb|Z37268 and gb|T88189 come from this |
| gene similar to SKP1 interacting partner 2 (SKIP2) TIGR_Ath1: At5g67250 (68414.m02724) | |||||
| 1104 | Not regulated | NC-NC-NC | 262501_at | At1g21690 | replication factor C 37 kDa, putative Similar to SWISS-PROT: P35249 activator 1 37 kDa subunit (Replication factor C 37 kDa |
| subunit, A1 37 kDa subunit, RF-C 37 kDa subunit, RFC37) [Homo sapiens]; contains Pfam domain, PF00004: ATPase, AAA family | |||||
| (88414.m02714) | |||||
| 1105 | Not regulated | NC-NC-NC | 262503_at | At1g21670 | expressed protein similar to TolB protein precursor (SP: P50601) {Pseudomonas aeruginosa} (68414.m02712) |
| 1106 | Not regulated | NC-NC-NC | 262451_at | At1g11140 | |
| 1107 | Not regulated | NC-NC-NC | 262453_at | At1g11240 | expressed protein (68414.m01287) |
| 1108 | Not regulated | NC-NC-NC | 262455_at | At1g11310 | seven transmembrane MLO family protein/MLO-like protein 2 (MLO2) identical to membrane protein Mlo2 [Arabidopsis thaliana] |
| gi|14091574|gb|AAK53795; similar to Mlo [Hordeum vulgare subsp. vulgare] gi|1877221|emb|CAB06083 SWISS-PROT: P93766 | |||||
| (68414.m01299) | |||||
| 1109 | Not regulated | NC-NC-NC | 262457_at | At1g11200 | expressed protein contains Pfam profile PF03619: Domain of unknown function (68414.m01283) |
| 1110 | Not regulated | NC-NC-NC | 262458_at | At1g11280 | S-locus protein kinase, putative similar to receptor protein kinase [Ipomoea trifida] gi|836954|gb|AAC23542; contains protein kinase |
| domain, Pfam: PF00069; contains S-locus glycoprotein family domain, Pfam: PF00954 (68414.m01294) | |||||
| 1111 | Not regulated | NC-NC-NC | 262473_at | At1g50250 | cell division protein ftsH homolog 1, chloroplast (FTSH1) (FTSH) identical to SP: Q39102 Cell division protein ftsH homolog 1. |
| chloroplast precursor (EC 3.4.24.—) [Arabidopsis thaliana] (68414.m05634) | |||||
| 1112 | Not regulated | NC-NC-NC | 262476_at | At1g50370 | serine/threonine protein phosphatase, putative nearly identical to serine/threonine protein phosphatase [Arabidopsis thaliana] |
| GI: 14582206 (68414.m05646) | |||||
| 1113 | Not regulated | NC-NC-NC | 262417_at | At1g50170 | expressed protein similar to Cbi protein (GI: 3724039) [Bacillus megaterium]; similar to YlnE protein (GI: 2462961) [Bacillus subtilis]; |
| similar to NirR (GI: 4433637) [Staphylococcus camosus] (68414.m05626) | |||||
| 1114 | Not regulated | NC-NC-NC | 262418_at | At1g50320 | thioredoxin X nearly identical to thioredoxin X GB: AAF15952 GI: 6539616 from [Arabidopsis thaliana] (68414.m05641) |
| 1115 | Not regulated | NC-NC-NC | 262422_at | At1g23440 | pyrrolidone-carboxylate peptidase family protein similar to Pyrrolidone-carboxylate peptidase (Swiss-Prot: O58321) [Pyrococcus |
| horikoshii]; contains Prosite PS00141: Eukaryotic and viral aspartyl proteases active site (68414.m02936) | |||||
| 1116 | Not regulated | NC-NC-NC | 262426_s_at | At1g47630 | |
| 1117 | Not regulated | NC-NC-NC | 262427_s_at | At1g47600 | glycosyl hydrolase family 1 protein contains Pfam PF00232: Glycosyl hydrolase family 1 domain; TIGRFAM TIGR01233: 6- |
| phospho-beta-galactosidase; similar to thioglucosidase (GI: 871992) [Arabidopsis thaliana] (68414.m05285) | |||||
| 1118 | Not regulated | NC-NC-NC | 262432_at | At1g47530 | ripening-responsive protein, putative similar to ripening regulated protein DDTFR18 [Lycopersicon esculentum] GI: 12231296; |
| contains Pfam profile PF01554: Uncharacterized membrane protein family (68414.m05275) | |||||
| 1119 | Not regulated | NC-NC-NC | 262434_at | At1g47670 | amino acid transporter family protein similar to lysine and histidine specific transporter GI: 2576361 from [Arabidopsis thaliana]; |
| contains Pfam profile PF01490: Transmembrane amino acid transporter protein (68414.m05296) | |||||
| 1120 | Not regulated | NC-NC-NC | 262442_at | At1g47420 | expressed protein identical to hypothetical protein GB: AAD46040 GI: 5668814 from [Arabidopsis thaliana] (68414.m05252) |
| 1121 | Not regulated | NC-NC-NC | 262414_at | At1g49430 | long-chain-fatty-acid--CoA ligase/long-chain acyl-CoA synthetase nearly identical to acyl CoA synthetase (MF45P) GI: 1617268 |
| from [Brassica napus] (68414.m05541) | |||||
| 1122 | Not regulated | NC-NC-NC | 262415_at | At1g49400 | ribosomal protein S17 family protein similar to 40S ribosomal protein S17 GI: 1620985 from [Nicotiana plumbaginitolia] |
| (68414.m05537) | |||||
| 1123 | Not regulated | NC-NC-NC | 262388_at | At1g49320 | BURP domain-containing protein similarity to SP|Q08298 Dehydration-responsive protein RD22 precursor {Arabidopsis thaliana}; |
| contains Pfam profile PF03181: BURP domain (68414.m05528) | |||||
| 1124 | Not regulated | NC-NC-NC | 262394_at | At1g49510 | expressed protein (68414.m05549) |
| 1125 | Not regulated | NC-NC-NC | 262396_at | At1g49470 | expressed protein contains Pfam profile PF04819: Family of unknown function (DUF716) (Plant viral-response family) |
| (68414.m05544) | |||||
| 1126 | Not regulated | NC-NC-NC | 262397_at | At1g49380 | cytochrome c biogenesis protein family contains Pfam PF05140: ResB-like family; similar to CCS1 (GI: 2749939) [Chlamydomonas |
| reinhardtii]; similar to Ccs1 (GI: 6137102) [Synechococcus sp. PCC 6301] (68414.m05535) | |||||
| 1127 | Not regulated | NC-NC-NC | 262400_at | At1g49480 | transcriptional factor B3 family protein contains Pfam profile PF02362: B3 DNA binding domain (68414.m05546) |
| 1128 | Not regulated | NC-NC-NC | 262401_at | At1g49300 | Ras-related GTP-binding protein, putative contains Pfam profile: PF00071 Ras family (68414.m05526) |
| 1129 | Not regulated | NC-NC-NC | 262407_at | At1g34630 | expressed protein (68414.m04305) |
| 1130 | Not regulated | NC-NC-NC | 262408_at | At1g34750 | protein phosphatase 2C, putative/PP2C, putative similar to GB: AAD17805 from (Lotus japonicus) (Proc. Natl. Acad. Sci. U.S.A. 96 |
| (4), 1738-1743 (1999)) (68414.m04321) | |||||
| 1131 | Not regulated | NC-NC-NC | 262413_at | At1g34780 | protein disulfide isomerase-related contains weak similarity to Pfam: P08003 protein disulfide isomerase A4 precursor (Protein ERp- |
| 72, ERp72) [Mus musculus] (68414.m04329) | |||||
| 1132 | Not regulated | NC-NC-NC | 262355_at | At1g72820 | mitochondrial substrate carrier family protein contains Pfam profile: PF00153 mitochondrial carrier protein (68414.m08419) |
| 1133 | Not regulated | NC-NC-NC | 262367_at | At1g73030 | SNF7 family protein contains Pfam domain, PF03357: SNF7 family (68414.m08445) |
| 1134 | Not regulated | NC-NC-NC | 262368_at | At1g73060 | expressed protein (68414.m08448) |
| 1135 | Not regulated | NC-NC-NC | 262354_at | At1g64200 | vacuolar ATP synthase subunit E, putative/V-ATPase E subunit, putative/vacuolar proton pump E subunit, putative similar to |
| SP|Q39258 Vacuolar ATP synthase subunit E (EC 3.6.3.14) (V-ATPase E subunit) (Vacuolar proton pump E subunit) {Arabidopsis | |||||
| thaliana}; contains Pfam profile PF01991: ATP synthase (E/31 kDa) subunit (68414.m07273) | |||||
| 1136 | Not regulated | NC-NC-NC | 262329_at | At1g64090 | reticulon family protein (RTNLB3) weak similarity to SP|O95197 Reticulon protein 3 (Neuroendocrine-specific protein-like) {Homo |
| sapiens}; contains Pfam profile PF02453: Reticulon (68414.m07260) | |||||
| 1137 | Not regulated | NC-NC-NC | 262340_at | At1g64105 | no apical meristem (NAM) family protein contains Pfam PF02365: No apical meristem (NAM) domain (68414.m07262) |
| 1138 | Not regulated | NC-NC-NC | 262343_at | At1g64040 | serine/threonine protein phosphatase PP1 isozyme 3 (TOPP3)/phosphoprotein phosphatase 1 identical to SP|P48483 |
| Serine/threonine protein phosphatase PP1 isozyme 3 (EC 3.1.3.16) {Arabidopsis thaliana}, phosphoprotein phosphatase 1 | |||||
| GB: AAA32838 GI: 166799 from [Arabidopsis thaliana] (68414.m07254) | |||||
| 1139 | Not regulated | NC-NC-NC | 262346_at | At1g63980 | D111/G-patch domain-containing protein contains Pfam profile PF01585: G-patch domain (68414.m07247) |
| 1140 | Not regulated | NC-NC-NC | 262349_at | At2g48130 | protease inhibitor/seed storage/lipid transfer protein (LTP) family protein contains Pfam protease inhibitor/seed storage/LTP family |
| domain PF00234 (68415.m06025) | |||||
| 1141 | Not regulated | NC-NC-NC | 262319_s_at | At1g27540 | F-box family protein similar to F-box protein family, AtFBX7 (GI: 20197899) [Arabidopsis thaliana]; confirmed by FLcDNA |
| GI: 16604421; contains uncharacterized Arabidoppsis domain shared by 33 Arabidopsis proteins (68414.m03357) | |||||
| 1142 | Not regulated | NC-NC-NC | 262297_at | At1g27600 | glycosyl transferase family 43 protein similar to Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Rattus |
| norvegicus [SP|O35789], Homo sapiens [SP|Q9P2W7]; contains Pfam domain Glycosyltransferase family 43 [PF03380] | |||||
| (68414.m03367) | |||||
| 1143 | Not regulated | NC-NC-NC | 262298_at | At1g27530 | expressed protein Similar to gb|AF151884 CGI-126 protein from Homo sapiens. EST gb|Z18048 comes from this gene |
| (68414.m03356) | |||||
| 1144 | Not regulated | NC-NC-NC | 262304_at | At1g70890 | major latex protein-related/MLP-related low similarity to major latex protein {Papaver somniferum}[GI: 294062]; contains Pfam |
| profile PF00407: Pathogenesis-related protein Bet v I family (68414.m08179) | |||||
| 1145 | Not regulated | NC-NC-NC | 262306_s_at | At1g70980 | asparaginyl-tRNA synthetase, cytoplasmic, putative/asparagine-tRNA ligase, putative similar to SYNC1 protein GI: 5670315 |
| [SP|Q9SW96] from [Arabidopsis thaliana] (68414.m08188) | |||||
| 1146 | Not regulated | NC-NC-NC | 262308_at | At1g71010 | phosphatidylinositol-4-phosphate 5-kinase family protein low similarity to phosphatidylinositol 3,5-kinase [Candida albicans] |
| GI: 14571648; contains Pfam profile PF01504: Phosphatidylinositol-4-phosphate 5-Kinase (68414.m08192) | |||||
| 1147 | Not regulated | NC-NC-NC | 262312_at | At1g70830 | Bet v I allergen family protein similar to Csf-2 [Cucumis sativus][GI: 5762258][J Am Soc Hortic Sci 124, 136-139 (1999)]; contains |
| Pfam profile PF00407: Pathogenesis-related protein Bet v I family (68414.m08170) | |||||
| 1148 | Not regulated | NC-NC-NC | 262288_at | At1g70760 | inorganic carbon transport protein-related contains weak similarity to Swiss-Prot: P27372 inorganic carbon transport protein |
| [Synechocystis sp.] (68414.m08156) | |||||
| 1149 | Not regulated | NC-NC-NC | 262289_at | At1g70770 | expressed protein (68414.m08158) |
| 1150 | Not regulated | NC-NC-NC | 262260_at | At1g70850 | Bet v I allergen family protein similar to Csf-2 [Cucumis sativus][GI: 5762258][J Am Soc Hortic Sci 124, 136-139 (1999)] contains |
| Pfam profile PF00407: Pathogenesis-related protein Bet v I family (68414.m08173) | |||||
| 1151 | Not regulated | NC-NC-NC | 262280_at | At1g68580 | agenet domain-containing protein/bromo-adjacent homology (BAH) domain-containing protein contains Pfam profile PF01426; |
| BAH domain and PF05641: Agenet domain (68414.m07835) | |||||
| 1152 | Not regulated | NC-NC-NC | 262281_at | At1g68570 | proton-dependent oligopeptide transport (POT) family protein contains Pfam profile: PF00854 POT family (68414.m07834) |
| 1153 | Not regulated | NC-NC-NC | 262283_at | At1g68590 | plastid-specific 30S ribosomal protein 3, putative/PSRP-3, putative similar to SP|P82412 Plastid-specific 30S ribosomal protein 3, |
| chloroplast precursor (PSRP-3) {Spinacia oleracea}; contains Pfam profile PF04839: Plastid and cyanobacterial ribosomal protein | |||||
| (PSRP-3/Ycf65) (68414.m07838) | |||||
| 1154 | Not regulated | NC-NC-NC | 262286_at | At1g68585 | expressed protein (68414.m07837) |
| 1155 | Not regulated | NC-NC-NC | 262237_at | At1g48320 | thioesterase family protein similar to ComAB [Bacillus licheniformis] GI: 1834379; contains Pfam profile PF03061: thioesterase |
| family protein (68414.m05397) | |||||
| 1156 | Not regulated | NC-NC-NC | 262251_at | At1g53760 | expressed protein (68414.m06117) |
| 1157 | Not regulated | NC-NC-NC | 262253_s_at | At1g53880 | eukaryotic translation initiation factor 2B family protein/elF-2B family protein similar to SP|Q64270 Translation initiation factor elF- |
| 2B alpha subunit {Rattus norvegicus}; contains Pfam profiles PF04525: Protein of unknown function (DUF567), PF01008: Initiation | |||||
| factor 2 subunit family (68414.m06133) | |||||
| 1158 | Not regulated | NC-NC-NC | 262254_at | At1g53920 | GDSL-motif lipase/hydrolase family protein similar to Anther-specific proline-rich proteins SP|P40603 SP|P40602 from {Arabidopsis |
| thaliana}; contains Pfam profile PF00657: GDSL-like Lipase/Acylhydrotase (68414.m06138) | |||||
| 1159 | Not regulated | NC-NC-NC | 262258_at | At1g53850 | 20S proteasome alpha subunit E1 (PAE1) identical to 20S proteasome subunit PAE1 GI: 3421087 from [Arabidopsis thaliana] |
| (68414.m06129) | |||||
| 1160 | Not regulated | NC-NC-NC | 262225_at | At1g53840 | pectinesterase family protein contains Pfam profile: PF01095 pectinesterase (68414.m06128) |
| 1161 | Not regulated | NC-NC-NC | 262203_at | At2g01060 | myb family transcription factor contains Pfam profile: PF00249 myb-like DNA-binding domain (68415.m00011) |
| 1162 | Not regulated | NC-NC-NC | 262204_at | At2g01100 | expressed protein (68415.m00016) |
| 1163 | Not regulated | NC-NC-NC | 262168_at | At1g74730 | expressed protein (68414.m08659) |
| 1164 | Not regulated | NC-NC-NC | 262172_at | At1g74970 | ribosomal protein S9 (RPS9) identical to ribosomal protein S9 [Arabidopsis thaliana] GI: 5456946 (68414.m08703) |
| 1165 | Not regulated | NC-NC-NC | 262173_at | At1g74920 | betaine-aldehyde dehydrogenase, putative identical to betaine-aldehyde dehydrogenase, chloroplast precursor (BADH) |
| [Arabidopsis thaliana] SWISS-PROT: Q9S795; strong similarity to betaine aldehyde dehydrogenase [Amaranthus hypochondriacus] | |||||
| GI: 2388710 (68414.m08691) | |||||
| 1166 | Not regulated | NC-NC-NC | 262174_at | At1g74910 | ADP-glucose pyrophosphorylase family protein contains Pfam profile PF00483: Nucleotidyl transferase; low similarity to mannose-1- |
| phosphate guanylyltransferase [Hypocrea jecorina] GI: 3323397 (68414.m08685) | |||||
| 1167 | Not regulated | NC-NC-NC | 262175_at | At1g74880 | expressed protein (68414.m08679) |
| 1168 | Not regulated | NC-NC-NC | 262176_at | At1g74960 | 3-ketoacyl-ACP synthase, putative similar to 3-ketoacyl-ACP synthase [Cuphea pulchemma] gi|3800747|gb|AAC68860; identical to |
| cDNA beta-ketoacyl-ACP synthetase 2 nuclear gene for plastid product GI: 14582700 (68414.m08699) | |||||
| 1169 | Not regulated | NC-NC-NC | 262180_at | At1g78050 | phosphoglycerate/bisphosphoglycerate mutase family protein similar to SP|P31217 Phosphoglycerate mutase 1 (EC 5.4.2.1) |
| {Escherichia coli O157: H7}; contains Pfam profile PF00300: phosphoglycerate mutase family (68414.m09095) | |||||
| 1170 | Not regulated | NC-NC-NC | 262181_at | At1g78060 | glycosyl hydrolase family 3 protein similar to xylosidase GI: 2102655 from [Aspergillus niger] (68414.m09096) |
| 1171 | Not regulated | NC-NC-NC | 262195_at | At1g78040 | pollen Ole a 1 allergen and extensin family protein contains Pfam domain, PF01190: Pollen proteins Ole a I family (68414.m09094) |
| 1172 | Not regulated | NC-NC-NC | 262137_at | At1g77920 | bZIP family transcription factor contains Pfam profile: PF00170 bZIP transcription factor (68414.m09080) |
| 1173 | Not regulated | NC-NC-NC | 262145_at | At1g52730 | transducin family protein/WD-40 repeat family protein contains 7 WD-40 repeats (PF00400); similar to UNR-interacting protein |
| (WD-40 repeat protein PT-WD) (SP: Q9Y3F4) [Homo sapiens] (68414.m05958) | |||||
| 1174 | Not regulated | NC-NC-NC | 262157_at | At1g52670 | biotin/lipoyl attachment domain-containing protein similar to SP|Q06881 Biotin carboxyl carrier protein of acetyl-CoA carboxylase |
| (BCCP) {Anabaena sp.}; contains Pfam profile PF00364: Biotin-requiring enzyme (68414.m05947) | |||||
| 1175 | Not regulated | NC-NC-NC | 262159_at | At1g52720 | expressed protein (68414.m05956) |
| 1176 | Not regulated | NC-NC-NC | 262160_at | At1g52590 | expressed protein (68414.m05937) |
| 1177 | Not regulated | NC-NC-NC | 262161_at | At1g52600 | signal peptidase, putative similar to SP|P13679 Microsomal signal peptidase 21 kDa subunit (EC 3.4.—.—) {Canis familians}; contains |
| Pfam profile PF00461: Signal peptidase I (68414.m05938) | |||||
| 1178 | Not regulated | NC-NC-NC | 262107_at | At1g02750 | drought-responsive family protein contains similarity to drought-induced mRNA, Di19 [Arabidopsis thaliana] |
| gi|469110|emb|CAA55321 (68414.m00229) | |||||
| 1179 | Not regulated | NC-NC-NC | 262109_at | At1g02730 | cellulose synthase family protein similar to cellulose synthase catalytic subunit [gi: 13925881] from Nicotiana alata, cellulose |
| synthase-4 [gi: 9622880] from Zea mays (68414.m00226) | |||||
| 1180 | Not regulated | NC-NC-NC | 262110_at | At1g02840 | pre-mRNA splicing factor SF2 (SF2)/SR1 protein identical to SP|O22315 Pre-mRNA splicing factor SF2 (SR1 protein) (Arabidopsis |
| thaliana) (68414.m00244) | |||||
| 1181 | Not regulated | NC-NC-NC | 262112_at | At1g02870 | expressed protein (68414.m00252) |
| 1182 | Not regulated | NC-NC-NC | 262113_at | At1g02820 | late embryogenesis abundant 3 family protein/LEA3 family protein similar to late embryogenis abundant protein 5 GI: 2981167 |
| from [Nicotiana tabacum]; contains Pfam profile PF03242: Late embryogenesis abundant protein (68414.m00242) | |||||
| 1183 | Not regulated | NC-NC-NC | 262114_at | At1g02860 | SPX (SYG1/Pho81/XPR1) domain-containing protein/zinc finger (C3HC4-type RING finger) protein-related weak similarity to |
| tripartite motif protein TRIM13 [Mus musculus] GI: 12407427, gpStaf50 [Homo sapiens] GI: 899300; contains Pfam profiles | |||||
| PF03105: SPX domain, PF00097: Zinc finger, C3HC4 type (RING finger) (68414.m00251) | |||||
| 1184 | Not regulated | NC-NC-NC | 262118_at | At1g02816 | expressed protein contains Pfam profile PF04398: Protein of unknown function, DUF538 (68414.m00241) |
| 1185 | Not regulated | NC-NC-NC | 262117_at | At1g02780 | 60S ribosomal protein L19 (RPL19A) similar to ribosomal protein L19 GI: 36127 from [Homo sapiens] (68414.m00233) |
| 1186 | Not regulated | NC-NC-NC | 262118_at | At1g02850 | glycosyl hydrolase family 1 protein contains Pfam PF00232: Glycosyl hydrolase family 1 domain; TIGRFAM TIGR01233: 6- |
| phospho-beta-galactosidase; similar to hydroxyisourate hydrolase (GI: 19569603) [Glycine max] (68414.m00247) | |||||
| 1187 | Not regulated | NC-NC-NC | 262077_at | At1g59610 | dynamin-like protein, putative (ADL3) strong similarity to dynamin-like protein 6 (ADL6) [Arabidopsis |
| thaliana] GI: 6651399; contains Pfam profiles PF01031: Dynamin central region, PF00350: Dynamin family, PF02212: Dynamin | |||||
| GTPase effector domain, | |||||
| PF00169: PH domain; identical to cDNA dynamin-like protein ADL3, GI: 4803835 (68414.m06704) | |||||
| 1188 | Not regulated | NC-NC-NC | 262078_at | At1g59520 | expressed protein (CW7) (68414.m06684) |
| 1189 | Not regulated | NC-NC-NC | 262096_at | At1g56010 | transcription activator NAC1 (NAC1) contains Pfam PF02365: No apical meristem (NAM) domain; identical to NAC1 GB: AAF21437 |
| GI: 6649236 from [Arabidopsis thaliana] (68414.m06427) | |||||
| 1190 | Not regulated | NC-NC-NC | 262064_at | At1g56075 | |
| 1191 | Not regulated | NC-NC-NC | 262039_at | At1g80050 | adenine phosphoribosyltransferase 2 (APT2) identical to SP|Q42563 Adenine phosphoribosyltransferase 2 (EC 2.4.2.7) (APRT) |
| {Arabidopsis thaliana} (68414.m09371) | |||||
| 1192 | Not regulated | NC-NC-NC | 262044_s_at | At1g80210 | expressed protein (68414.m09387) |
| 1193 | Not regulated | NC-NC-NC | 262048_at | At1g80230 | cytochrome c oxidase family protein contains Pfam domain, PF01215: Cytochrome c oxidase subunit Vb (68414.m09389) |
| 1194 | Not regulated | NC-NC-NC | 262053_at | At1g79940 | DNAJ heat shock N-terminal domain-containing protein/sec63 domain-containing protein similar to SP|Q9UGP8 Translocation |
| protein SEC63 homolog {Homo sapiens}; contains Pfam profiles PF00226 DnaJ domain, PF02889 Sec63 domain (68414.m09342) | |||||
| 1195 | Not regulated | NC-NC-NC | 262055_at | At1g79970 | expressed protein (68414.m09348) |
| 1196 | Not regulated | NC-NC-NC | 262057_at | At1g80040 | expressed protein (68414.m09369) |
| 1197 | Not regulated | NC-NC-NC | 262058_at | At1g79975 | expressed protein (68414.m09352) |
| 1198 | Not regulated | NC-NC-NC | 262059_at | At1g80030 | DNAJ heat shock protein, putative similar to SP|Q05646 Chaperone protein dnaJ {Erysipelothrix rhusiopathiae}; contains Pfam |
| profiles PF00226: DnaJ domain. PF01556: DnaJ C terminal region, PF00684: DnaJ central domain (4 repeats) (68414.m09366) | |||||
| 1199 | Not regulated | NC-NC-NC | 262038_at | At1g35580 | beta-fructofuranosidase, putative/invertase, putative/saccharase, putative/beta-fructosidase, putative similar to neutral invertase |
| [Daucus carota] GI: 4200165; contains Pfam profile PF04853: Plant neutral invertase (68414.m04417) | |||||
| 1200 | Not regulated | NC-NC-NC | 262026_at | At1g35670 | calcium-dependent protein kinase 2 (CDPK2) identical to calcium-dependent protein kinase [Arabidopsis thaliana] |
| gi|604881|dbj|BAA04830; contains protein kinase domain, Pfam: PF00069; contains EF hand domain (calcium-binding EF-hand). | |||||
| Pfam: PF00036, INTERPRO: IPR002048 (68414.m04435) | |||||
| 1201 | Not regulated | NC-NC-NC | 262029_at | At1g35880 | 50S ribosomal protein L21, chloroplast/CL21 (RPL21) identical to 50S ribosomal protein L21, chloroplast precursor (CL21) |
| {Arabidopsis thaliana} SWISS-PROT: P51412 (68414.m04436) | |||||
| 1202 | Not regulated | NC-NC-NC | 261981_at | At1g33811 | GDSL-motif lipase/hydrolase family protein similar to family II lipases EXL3 GI: 15054386, EXL1 GI: 15054382, EXL2 GI: 15054384 |
| from [Arabidopsis thaliana]; contains Pfam profile PF00657: GDSL-like Lipase/Acylhydrolase (68414.m04180) | |||||
| 1203 | Not regulated | NC-NC-NC | 261962_at | At1g33780 | expressed protein similar to At3g29240 [Arabidopsis thaliana]; contains Pfam profile PF02622: Uncharacterized ACR, COG1678 |
| (68414.m04175) | |||||
| 1204 | Not regulated | NC-NC-NC | 261999_at | At1g33800 | expressed protein contains Pfam profile PF04669: Protein of unknown function (DUF579) (68414.m04178) |
| 1205 | Not regulated | NC-NC-NC | 262005_at | At1g64550 | ABC transporter family protein similar to ABC transporter protein GB: AAF31030 GI: 6699653 from [Leishmania major] |
| (68414.m07317) | |||||
| 1206 | Not regulated | NC-NC-NC | 261972_at | At1g64600 | expressed protein similar to Hypothetical 72.2 kDa protein in RPS27A-GPM1 intergenic region (Swiss-Prot: P36056) |
| [Saccharomyces cerevisiae] (68414.m07322) | |||||
| 1207 | Not regulated | NC-NC-NC | 261945_at | At1g64530 | RWP-RK domain-containing protein similar to nodule inception protein [Lotus japonicus] GI: 6448579; contains Pfam profile: |
| PF02042 RWP-RK domain (68414.m07315) | |||||
| 1208 | Not regulated | NC-NC-NC | 261948_at | At1g64680 | expressed protein (68414.m07332) |
| 1209 | Not regulated | NC-NC-NC | 261949_at | At1g64670 | hydrolase, alpha/beta fold family protein low similarity to 2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase [Pseudomonas |
| putida] GI: 2822275; contains Pfam profile PF00561: hydrolase, alpha/beta fold family (68414.m07331) | |||||
| 1210 | Not regulated | NC-NC-NC | 261954_at | At1g64510 | ribosomal protein S6 family protein similar to plastid ribosomal protein S6 precursor GB: AAF64311 GI: 7582401 from [Spinacia |
| oleracea] (68414.m07313) | |||||
| 1211 | Not regulated | NC-NC-NC | 261955_at | At1g64520 | 26S proteasome regulatory subunit, putative (RPN12) similar to 26S proteasome regulatory complex subunit p30 GB: AAF08395 |
| GI: 6434966 from [Drosophila melanogaster] (68414.m07314) | |||||
| 1212 | Not regulated | NC-NC-NC | 261958_at | At1g64590 | short-chain dehydrogenase/reductase (SDR) family protein contains INTERPRO family IPR002198 short-chain |
| dehydrogenase/reductase (SDR) superfamily (68414.m07321) | |||||
| 1213 | Not regulated | NC-NC-NC | 261958_at | At1g64500 | glutaredoxin family protein (68414.m07312) |
| 1214 | Not regulated | NC-NC-NC | 261919_at | At1g65980 | peroxiredoxin type 2, putative strong similarity to type 2 peroxiredoxin [Brassica rapa subsp. pekinensis] GI: 4928472; contains |
| Pfam profile: PF00578 AhpC/TSA (alkyl hydroperoxide reductase and thiol-specific antioxidant) family (68414.m07486) | |||||
| 1215 | Not regulated | NC-NC-NC | 261920_at | At1g65930 | isocitrate dehydrogenase, putative/NADP+ isocitrate dehydrogenase, putative strong similarity to isocitrate dehydrogenase |
| SP|Q40345 from [Medicago sativa] (68414.m07481) | |||||
| 1216 | Not regulated | NC-NC-NC | 261924_at | At1g22550 | proton-dependent oligopeptide transport (POT) family protein contains Pfam profile: PF00854 POT family (68414.m02816) |
| 1217 | Not regulated | NC-NC-NC | 261925_at | At1g22540 | proton-dependent oligopeptide transport (POT) family protein contains Pfam profile: PF00854 POT family (68414.m02815) |
| 1218 | Not regulated | NC-NC-NC | 261930_at | At1g22440 | alcohol dehydrogenase, putative similar to alcohol dehydrogenase ADH GI: 7705214 from [Lycopersicon esculentum]; contains |
| Pfam zinc-binding dehydrogenase domain PF00107 (68414.m02805) | |||||
| 1219 | Not regulated | NC-NC-NC | 261939_at | At1g22450 | cytochrome c oxidase subunit 6b, putative (COX6b) nearly identical to subunit 6b of cytochrome c oxidase [Arabidopsis thaliana] |
| GI: 6518353 (68414.m02806) | |||||
| 1220 | Not regulated | NC-NC-NC | 261940_at | At1g22520 | expressed protein contains Pfam PF04418: Domain of unknown function (DUF543) (68414.m02813) |
| 1221 | Not regulated | NC-NC-NC | 261882_at | At1g80770 | expressed protein (68414.m09476) |
| 1222 | Not regulated | NC-NC-NC | 261885_at | At1g80930 | MIF4G domain-containing protein/MA3 domain-containing protein similar to SP|Q9P6R9 Cell cycle control protein cwf22 |
| {Schizosaccharomyces pombe}; contains Pfam profiles PF02854: MIF4G domain, PF02847: MA3 domain (68414.m09495) | |||||
| 1223 | Not regulated | NC-NC-NC | 261886_s_at | At1g80700 | expressed protein (68414.m09469) |
| 1224 | Not regulated | NC-NC-NC | 261887_at | At1g80780 | CCR4-NOT transcription complex protein, putative similar to SWISS-PROT: Q60809 CCR4-NOT transcription complex, subunit 7 |
| (CCR4-associated factor 1, (CAF1) [Mus musculus] (68414.m09477) | |||||
| 1225 | Not regulated | NC-NC-NC | 261891_at | At1g80790 | XH/XS domain-containing protein/XS zinc finger domain-containing protein contains Pfam domains PF03469: XH domain, |
| PF03468: XS domain and PF03470: XS zinc finger domain (68414.m09479) | |||||
| 1226 | Not regulated | NC-NC-NC | 261895_at | At1g80830 | NRAMP metal ion transporter 1 (NRAMP1) identical to NRAMP1 protein [Arabidopsis thaliana] gi|7108911|gb|AAF36535; member |
| of the natural resistance-associated macrophage protein (NRAMP) metal transporter family, PMID: 11500563 (68414.m09483) | |||||
| 1227 | Not regulated | NC-NC-NC | 261896_at | At1g80670 | transducin family protein/WD-40 repeat family protein contains 5 WD-40 repeats (PF00400) (1 weak); similar to Hypothetical |
| RAE1-like protein. (SP: Q38942) [Arabidopsis thaliana]; similar to mRNA-associated protein mmp 41 ((mRNA export protein) | |||||
| (GB: AAC28126) (GI: 1903456)(RAE1) (MRNP41) (SP: P78406) [Homo sapiens] (68414.m09486) | |||||
| 1228 | Not regulated | NC-NC-NC | 261897_at | At1g80950 | phospholipid/glycerol acyltransferase family protein low similarity to SP|Q59601 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC |
| 2.3.1.51) {Neisseria gonorrhoeae}; contains Pfam profile PF01553: Acyltransferase (68414.m09498) | |||||
| 1229 | Not regulated | NC-NC-NC | 261900_at | At1g80940 | expressed protein (68414.m09496) |
| 1230 | Not regulated | NC-NC-NC | 261902_at | At1g80860 | expressed protein (68414.m09486) |
| 1231 | Not regulated | NC-NC-NC | 261904_at | At1g65040 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) |
| (68414.m07372) | |||||
| 1232 | Not regulated | NC-NC-NC | 261852_at | At1g50440 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) |
| (68414.m05653) | |||||
| 1233 | Not regulated | NC-NC-NC | 261854_at | At1g50670 | OTU-like cysteine protease family protein contains Pfam profile PF02338: OTU-like cysteine protease (68414.m05698) |
| 1234 | Not regulated | NC-NC-NC | 261855_at | At1g50510 | indigoidine synthase A family protein similar to lndA protein (GI: 7576263) [Erwinia chrysanthemi]; contains Pfam profile PF04227: |
| Indigoidine synthase A like protein (68414.m05665) | |||||
| 1235 | Not regulated | NC-NC-NC | 261858_at | At1g50570 | C2 domain-containing protein low similarity to cold-regulated gene SRC2 [Glycine max] GI: 2055230; contains Pfam profile |
| PF00168: C2 domain (68414.m05675) | |||||
| 1236 | Not regulated | NC-NC-NC | 261860_at | At1g50600 | scarecrow-like transcription factor 5 (SCL5) similar to SCARECROW GB: AAB06318 GI: 1497987 from [Arabidopsis thaliana] |
| (68414.m05683) | |||||
| 1237 | Not regulated | NC-NC-NC | 261861_at | At1g50450 | expressed protein (68414.m05655) |
| 1238 | Not regulated | NC-NC-NC | 261864_s_at | At1g50480 | formate-tetrahydrofolate ligase/10-formyltetrahydrofolate synthetase (THFS) identical to 10-formyltetrahydrofolate synthetase |
| (Arabidopsis thaliana) GI: 5921663 (68414.m05660) | |||||
| 1239 | Not regulated | NC-NC-NC | 261865_at | At1g50430 | 7-dehydrocholesterol reductase/7-DHC reductase/sterol delta-7-reductase (ST7R)/dwarf5 protein (DWF5) identical to |
| SP|Q9LDU6 7-dehydrocholesterol reductase (EC 1.3.1.21) (7-DHC reductase) (Sterol delta-7-reductase) (Dwarf5 protein) | |||||
| {Arabidopsis thaliana} (68414.m05652) | |||||
| 1240 | Not regulated | NC-NC-NC | 261866_at | At1g50420 | scarecrow-like transcription factor 3 (SCL3) identical to GB: AAD24404 GI: 4580515 from [Arabidopsis thaliana] |
| (Plant J. 18 (1), 111-119 (1999)) (68414.m05651) | |||||
| 1241 | Not regulated | NC-NC-NC | 261867_at | At1g50575 | lysine decarboxylase family protein contains Pfam profile PF03641: lysine decarboxylase family (68414.m05677) |
| 1242 | Not regulated | NC-NC-NC | 261871_at | At1g11440 | expressed protein (68414.m01314) |
| 1243 | Not regulated | NC-NC-NC | 261821_at | At1g11530 | thioredoxin family protein similar to thioredoxin H-type from Arabidopsis thaliana SP|P29448, Nicotiana tabacum SP|Q07090; |
| contains Pfam profile: PF00085 Thioredoxin (68414.m01324) | |||||
| 1244 | Not regulated | NC-NC-NC | 261822_at | At1g11380 | expressed protein contains Pfam profile PF04749: Protein of unknown function, DUF614 (68414.m01307) |
| 1245 | Not regulated | NC-NC-NC | 261823_at | At1g11400 | expressed protein (68414.m01309) |
| 1246 | Not regulated | NC-NC-NC | 261824_at | At1g11430 | plastid developmental protein DAG, putative similar to DAG protein, chloroplast precursor [Garden snapdragon] SWISS- |
| PROT: Q38732 (68414.m01313) | |||||
| 1247 | Not regulated | NC-NC-NC | 261827_at | At1g11480 | eukaryotic translation initiation factor-related contains weak similarity to Swiss-Prot: P23588 eukaryotic translation initiation factor 4B |
| (eIF-4B) [Homo sapiens] (68414.m01319) | |||||
| 1248 | Not regulated | NC-NC-NC | 261828_at | At1g11360 | universal stress protein (USP) family protein contains Pfam PF00582: universal stress protein family domain; similar to ethylene- |
| responsive ER6 protein (GI: 5669654) [Lycopersicon esculentum] (68414.m01304) | |||||
| 1249 | Not regulated | NC-NC-NC | 261831_at | At1g10630 | ADP-ribosylation factor, putative similar to ADP-ribosylation factor GI: 166586 from [Arabidopsis thaliana] (68414.m01205) |
| 1250 | Not regulated | NC-NC-NC | 261832_at | At1g10650 | expressed protein (68414.m01207) |
| 1251 | Not regulated | NC-NC-NC | 261838_at | At1g16030 | heat shock protein 70, putative/HSP70, putative similar to heat shock protein hsp70 GI: 1771478 from [Pisum sativum] |
| (68414.m01924) | |||||
| 1252 | Not regulated | NC-NC-NC | 261843_at | At1g16180 | TMS membrane family protein/tumour differentially expressed (TDE) family protein contains Pfam domain, PF03348: TMS |
| membrane protein/tumour differentially expressed protein (TDE) (68414.m01938) | |||||
| 1253 | Not regulated | NC-NC-NC | 261787_at | At1g16020 | expressed protein (68414.m01921) |
| 1254 | Not regulated | NC-NC-NC | 261788_at | At1g15980 | expressed protein (68414.m01917) |
| 1255 | Not regulated | NC-NC-NC | 261790_at | At1g16000 | expressed protein (68414.m01919) |
| 1256 | Not regulated | NC-NC-NC | 261791_at | At1g16170 | expressed protein (68414.m01937) |
| 1257 | Not regulated | NC-NC-NC | 261793_at | At1g16080 | expressed protein (68414.m01929) |
| 1258 | Not regulated | NC-NC-NC | 261794_at | At1g16060 | ovule development protein, putative similar to ovule development protein AINTEGUMENTA (GI: 1209099)[Arabidopsis thaliana] |
| (68414.m01926) | |||||
| 1259 | Not regulated | NC-NC-NC | 261795_at | At1g16010 | magnesium transporter CorA-like family protein (MRS2-1) low similarity to SP|Q01926 RNA splicing protein MRS2, mitochondrial |
| precursor {Saccharomyces cerevisiae}; contains Pfam profile PF01544: CorA-like Mg2+ transporter protein (68414.m01920) | |||||
| 1260 | Not regulated | NC-NC-NC | 261801_at | At1g30520 | acyl-activating enzyme 14 (AAE14) identical to acyl-activating enzyme 14 [Arabidopsis thaliana]; similar to SP|Q42524 4-coumarate- |
| CoA ligase 1 (EC 6.2.1.12) (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}; contains Pfam profile PF00501: AMP-binding | |||||
| enzyme; identical to cDNA acyl-activating enzyme 14 (At1g30520) GI: 29893263 (68414.m03734) | |||||
| 1261 | Not regulated | NC-NC-NC | 261806_at | At1g30510 | ferredoxin--NADP(+) reductase, putative/adrenodoxin reductase, putative strong similarity to SP|P41345 Ferredoxin--NADP |
| reductase, root isozyme, chloroplast precursor (EC 1.18.1.2) (FNR) {Oryza sativa}, ferredoxin-NADP reductase precursor [Zea | |||||
| mays] GI: 500751 (68414.m03730) | |||||
| 1262 | Not regulated | NC-NC-NC | 261808_at | At1g30480 | DNA-damage-repair/toleration protein, chloroplast (DRT111) nearly identical to SP|P42698 DNA-damage-repair/toleration protein |
| DRT111, chloroplast precursor {Arabidopsis thaliana}; contains Pfam profiles PF01585: G-patch domain, PF00076: RNA | |||||
| recognition motif. (a.k.a. RRM, RBD, or RNP domain) (68414.m03726) | |||||
| 1263 | Not regulated | NC-NC-NC | 261810_at | At1g08130 | DNA ligase/polydeoxyribonucleotide synthase [ATP] identical to SP|Q42572 DNA ligase (EC 6.5.1.1) {Polydeoxyribonucleotide |
| synthase [ATP]} {Arabidopsis thaliana}; contains Pfam profiles: PF01068 ATP dependent DNA ligase domain, PF04679 ATP | |||||
| dependent DNA ligase C terminal region, PF04675 DNA ligase N terminus (68414.m00892) | |||||
| 1264 | Not regulated | NC-NC-NC | 261815_at | At1g08325 | |
| 1265 | Not regulated | NC-NC-NC | 261756_at | At1g08320 | bZIP family transcription factor contains Pfam profile: PF00170 bZIP transcription factor (68414.m00920) |
| 1266 | Not regulated | NC-NC-NC | 261757_at | At1g08210 | aspartyl protease family protein contains Pfam profile PF00026: Eukaryotic aspartyl protease; similar to CND41, chloroplast |
| nucleoid DNA binding protein (GI: 2541876) {Nicotiana tabacum} (68414.m00907) | |||||
| 1267 | Not regulated | NC-NC-NC | 261758_at | At1g08250 | prephenate dehydratase family protein contains similarity to prephenate dehydratase GI: 1008717 from [Amycolatopsis |
| methanolica] (68414.m00910) | |||||
| 1268 | Not regulated | NC-NC-NC | 261769_at | At1g76100 | plastocyanin identical to plastocyanin GI: 1865683 from [Arabidopsis thaliana] (68414.m08837) |
| 1269 | Not regulated | NC-NC-NC | 261770_at | At1g76140 | prolyl oligopeptidase, putative/prolyl endopeptidase, putative/post-proline cleaving enzyme, putative similar to SP|Q9QUR6 Prolyl |
| endopeptidase (EC 3.4.21.26) (Post-proline cleaving enzyme) {Mus musculus}; contains Pfam profiles PF00326: prolyl | |||||
| oligopeptidase family, PF02897: Prolyl oligopeptidase, N-terminal beta-propeller domain (68414.m08842) | |||||
| 1270 | Not regulated | NC-NC-NC | 261771_at | At1g76150 | maoC-like dehydratase domain-containing protein contains similarity to Swiss-Prot: P51659 estradiol 17 beta-dehydrogenase 4 (17- |
| beta-HSD 4) (17-beta-hydroxysteroid dehydrogenase 4) [Homo sapiens]; contains Pfam profile PF01575: MaoC like domain | |||||
| (68414.m08843) | |||||
| 1271 | Not regulated | NC-NC-NC | 261748_at | At1g76070 | expressed protein (68414.m08834) |
| 1272 | Not regulated | NC-NC-NC | 261751_at | At1g76080 | thioredoxin family protein low similarity to thioredoxin (TRX) [Fasciola hepatica] GI: 6687568; contains Pfam profile PF00085: |
| Thioredoxin (68414.m08835) | |||||
| 1273 | Not regulated | NC-NC-NC | 261754_at | At1g76130 | alpha-amylase, putative/1,4-alpha-D-glucan glucanohydrolase, putative strong similarity to alpha-amylase GI: 7532799 from [Malus x |
| domestica]; contains Pfam profile PF00128: Alpha amylase, catalytic domain (68414.m08841) | |||||
| 1274 | Not regulated | NC-NC-NC | 261723_at | At1g76200 | expressed protein (68414.m08849) |
| 1275 | Not regulated | NC-NC-NC | 261725_at | At1g76300 | small nuclear ribonucleoprotein D3, putative/snRNP core protein D3, putative/Sm protein D3, putative similar to SWISS- |
| PROT: P43331 small nuclear ribonucleoprotein Sm D3 (snRNP core protein D3, Sm-D3) [Mouse] (68414.m08862) | |||||
| 1276 | Not regulated | NC-NC-NC | 261733_at | At1g47830 | clathrin coat assembly protein, putative similar to clathrin coat assembly protein AP17 GB: CAA65533 GI: 2959358 from [Zea mays]; |
| contains Pfam profile: PF01217 clathrin adaptor complex small chain (68414.m05324) | |||||
| 1277 | Not regulated | NC-NC-NC | 261739_at | At1g47750 | peroxisomal biogenesis factor 11 family protein/PEX11 family protein contains Pfam PF05648: Peroxisomal biogenesis factor 11 |
| (PEX11) (68414.m05310) | |||||
| 1278 | Not regulated | NC-NC-NC | 261740_at | At1g47740 | expressed protein (68414.m05308) |
| 1279 | Not regulated | NC-NC-NC | 261744_at | At1g08490 | cysteine desulfurase, putative similar to nitrogen fixation protein (nifS) GB: D64004 GI: 1001701 from [Synechocystis sp]; contains |
| TIGRFAM TIGR01364: phosphoserine aminotransferase; contains Pfam PF00266: aminotransferase, class V (68414.m00940) | |||||
| 1280 | Not regulated | NC-NC-NC | 261746_at | At1g08380 | expressed protein (68414.m00927) |
| 1281 | Not regulated | NC-NC-NC | 261720_at | At1g08460 | histone deacetylase family protein (HDA8) identical to HDA8 [Arabidopsis thaliana] GI: 21360988low similarity to SP|Q9Z2V5 |
| Histone deacetylase 6 (ND6) (Histone deacetylase mHDA2) {Mus musculus}; contains Pfam profile PF00850: Histone deacetylase | |||||
| family; supporting cDNA gi|21360987|gb|AF510167.1| (68414.m00936) | |||||
| 1282 | Not regulated | NC-NC-NC | 261721_at | At1g08480 | expressed protein (68414.m00939) |
| 1283 | Not regulated | NC-NC-NC | 261692_at | At1g08450 | catreticulin 3 (CRT3) identical to similar to SP|O04153 Calreticulin 3 precursor {Arabidopsis thaliana} (68414.m00934) |
| 1284 | Not regulated | NC-NC-NC | 261693_at | At1g08370 | hydroxyproline-rich glycoprotein family protein contains proline-rich extensin domains, INTERPRO: IPR002965; contains some |
| similarity to transcription factor [Danio rerio] gi|15617376|emb|CAC69871 (68414.m00926) | |||||
| 1285 | Not regulated | NC-NC-NC | 261694_at | At1g08360 | 60S ribosomal protein L10A (RPL10aA) similar to 60S ribosomal protein L10A GB: AAC73045 GI: 3860277 from [Arabidopsis |
| thaliana] (68414.m00925) | |||||
| 1286 | Not regulated | NC-NC-NC | 261895_at | At1g08520 | magnesium-chelatase subunit chID, chloroplast, putative/Mg-protoporphyrin IX chelatase, putative (CHLD) similar to Mg-chelatase |
| SP|O24133 from Nicotiana tabacum, GB: AF014399 GI: 2318116 from [Pisum sativum] (68414.m00943) | |||||
| 1287 | Not regulated | NC-NC-NC | 261696_at | At1g08470 | strictosidine synthase family protein similar to strictosidine synthase [Rauvolfia serpentina][SP|P15324] (68414.m00938) |
| 1288 | Not regulated | NC-NC-NC | 261709_at | At1g32790 | RNA-binding protein, putative similar to RNA-binding protein GB: CAB40027 GI: 4539439 from [Arabidopsis thaliana] |
| (68414.m04042) | |||||
| 1289 | Not regulated | NC-NC-NC | 261711_at | At1g32700 | zinc-binding family protein similar to zinc-binding protein [Pisum sativum] GI: 16117799; contains Pfam profile PF04640: Protein of |
| unknown function, DUF597 (68414.m04032) | |||||
| 1290 | Not regulated | NC-NC-NC | 261718_at | At1g18390 | protein kinase family protein contains protein kinase domain, Pfam: PF00069 (68414.m02297) |
| 1291 | Not regulated | NC-NC-NC | 261661_at | At1g18360 | hydrolase, alpha/beta fold family protein similar to monoglyceride lipase from [Homo sapiens] GI: 14594904, [Mus musculus] |
| GI: 2632162, [Rattus norvegicus] GI: 19697886; contains Pfam profile PF00561: hydrolase, alpha/beta fold family (68414.m02294) | |||||
| 1292 | Not regulated | NC-NC-NC | 261666_at | At1g18440 | peptidyl-tRNA hydrolase family protein contains Pfam profile PF01195: peptidyl-tRNA hydrolase (68414.m02301) |
| 1293 | Not regulated | NC-NC-NC | 261687_at | At1g18460 | lipase family protein similar to triacylglycerol lipase, gastric precursor (EC 3.1.1.3) {Canis familiaris} [SP|P80035] (68414.m02303) |
| 1294 | Not regulated | NC-NC-NC | 261672_at | At1g18450 | actin-related protein 4 (ARP4) neary identical to actin-related protein 4 (ARP4) [Arabidopsis thaliana] GI: 21427463; contains Pfam |
| profile PF00022: Actin; supporting cDNA gi|21427462|gb|AF507912.1| (68414.m02302) | |||||
| 1295 | Not regulated | NC-NC-NC | 261674_at | At1g18270 | ketose-bisphosphate aldolase class-II family protein low similarity to KbaY (tagatose-1,6-bisphosphate aldolase) [Escherichia coli] |
| GI: 8895753; contains Pfam profile PF01116: Fructose-bisphosphate aldolase class-II (68414.m02280) | |||||
| 1296 | Not regulated | NC-NC-NC | 261677_at | At1g18470 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (68414.m02304) |
| 1297 | Not regulated | NC-NC-NC | 261682_at | At1g47330 | expressed protein contains Pfam profile PF01595: Domain of unknown function (68414.m05240) |
| 1298 | Not regulated | NC-NC-NC | 261659_at | At1g50030 | target of rapamycin protein (TOR) identical to pTOR [Arabidopsis thaliana] GI: 12002902; contains Pfam profiles PF00454 |
| Phosphatidylinositol 3- and 4-kinase, PF02259 FAT domain, PF02260 FATC domain (68414.m05614) | |||||
| 1299 | Not regulated | NC-NC-NC | 261634_at | At1g49970 | ATP-dependent Clp protease proteolytic subunit (ClpR1) (nClpP5) identical to nClpP5 GB: BAA82069 GI: 5380595 from |
| [Arabidopsis thaliana]; identical to cDNA nClpP5 (nuclear encoded ClpP5) GI: 5360594 (68414.m05607) | |||||
| 1300 | Not regulated | NC-NC-NC | 261635_at | At1g50020 | expressed protein (68414.m05613) |
| 1301 | Not regulated | NC-NC-NC | 261637_at | At1g49950 | DNA-binding protein, putative contains similarity to DNA-binding protein PcMYB1 [Petroselinum crispum] gi|2224899|gb|AAB81699 |
| (68414.m05602) | |||||
| 1302 | Not regulated | NC-NC-NC | 261638_at | At1g49975 | expressed protein (68414.m05608) |
| 1303 | Not regulated | NC-NC-NC | 261642_at | At1g27680 | glucose-1-phosphate adenylyltransferase large subunit 2 (APL2)/ADP-glucose pyrophosphorylase identical to SP|P55230 |
| (68414.m03383) | |||||
| 1304 | Not regulated | NC-NC-NC | 261644_s_at | At1g27830 | |
| 1305 | Not regulated | NC-NC-NC | 261655_at | At1g01940 | peptidyl-prolyl cis-trans isomerase cyclophilin-type family protein contains Pfam domain, PF00160: peptidyl-prolyl cis-trans |
| isomerase, cyclophilin-type (68414.m00112) | |||||
| 1306 | Not regulated | NC-NC-NC | 261622_at | At1g01970 | pentatricopeptide (PPR) repeat-containing protein low similarity to 67 kD chloroplastic RNA-binding protein RSP67.2 [Raphanus |
| sativus] GI: 9755888; contains Pfam profile PF01535: PPR repeat (68414.m00115) | |||||
| 1307 | Not regulated | NC-NC-NC | 261626_at | At1g01990 | expressed protein (68414.m00117) |
| 1308 | Not regulated | NC-NC-NC | 261627_at | At1g01910 | anion-transporting ATPase, putative similar to SP|O43681 Arsenical pump-driving ATPase (EC 3.6.3.16) (Arsenite-translocating |
| ATPase) (Arsenical resistance ATPase) (Arsenite-transporting ATPase) (ARSA) (ASNA-I) {Homo sapiens}; contains Pfam profile | |||||
| PF02374; Anion-transporting ATPase (68414.m00108) | |||||
| 1309 | Not regulated | NC-NC-NC | 261606_at | At1g49570 | peroxidase, putative identical to peroxidase ATP5a [Arabidopsis thaliana] gi|1546702|emb|CAA67341; similar to peroxidase SWISS |
| PROT: P80679 from [Armoracia rusticana] (68414.m05558) | |||||
| 1310 | Not regulated | NC-NC-NC | 261607_at | At1g49860 | expressed protein (68414.m05569) |
| 1311 | Not regulated | NC-NC-NC | 261612_at | At1g49710 | fucosyltransferase-like protein, putative/FucT2, putative/FucTB, putative (FUT12) identical to Putative fucosyltransferase-like |
| protein (FucTB) (FucT2) (AtFUT12) (Swiss-Prot: Q9FX97) [Arabidopsis thaliana]; similar to glycoprotain 3-alpha-L- | |||||
| fucosyltransferase A (SP: Q9LJK1) [Arabidopsis thaliana]; contains Pfam profile PF00852: Fucosyl transferase (68414.m05573) | |||||
| 1312 | Not regulated | NC-NC-NC | 261620_s_at | At1g33140 | 60S ribosomal protein L9 (RPL90A/C) similar to RIBOSOMAL PROTEIN L9 GB: P49209 from [Arabidopsis thaliana] |
| (68414.m04093) | |||||
| 1313 | Not regulated | NC-NC-NC | 261566_at | At1g33230 | expressed protein (68414.m04106) |
| 1314 | Not regulated | NC-NC-NC | 261570_at | At1g01120 | fatty acid elongase 3-ketoacyl-CoA synthase 1 (KCS1) nearly identical to GB: AAC99312 GI: 4091810 from [Arabidopsis thaliana] |
| (68414.m00015) | |||||
| 1315 | Not regulated | NC-NC-NC | 261577_at | At1g01080 | 33 kDa ribonucleoprotein, chloroplast, putative/RNA-binding protein cp33, putative similar to 33 KDA RIBONUCLEOPROTEIN |
| GB: P19684 from [Nicotiana sylvestris] (68414.m00010) | |||||
| 1316 | Not regulated | NC-NC-NC | 261582_at | At1g01160 | SSXT protein-related/transcription co-activator-related similar to SYT/SSX4 fusion protein (GI: 11127695) [Homo sapiens]; |
| supporting cDNA gi|21539891|gb|AY102640.1|; contains Pfam profile PF05030: SSXT protein (N-terminal region) (68414.m00026) | |||||
| 1317 | Not regulated | NC-NC-NC | 261583_at | At1g01090 | pyruvate dehydrogenase E1 component alpha subunit, chloroplast identical to pyruvate dehydrogenase E1 alpha subunit |
| GB: AAB86803 GI: 2454182 from [Arabidopsis thaliana]; identical to cDNA pyruvate dehydrogenase E1 alpha subunit mRNA, | |||||
| nuclear gene encoding plastid protein GI: 2454181 (68414.m00011) | |||||
| 1318 | Not regulated | NC-NC-NC | 261591_at | At1g01740 | protein kinase family protein low similarity to protein kinase [Arabidopsis thaliana] GI: 2852449; contains Pfam profile: PF00069 |
| Protein kinase domain (68414.m00093) | |||||
| 1319 | Not regulated | NC-NC-NC | 261563_at | At1g01630 | SEC14 cytosolic factor, putative/phosphoglyceride transfer protein, putative contains Pfam PF00650: CRAL/TRIO domain and |
| PF03765: CRAL/TRIO, N-terminus; similar to polyphosphoinositide binding protein Ssh2p GB: AAB94599 GI: 2739046 from | |||||
| [Glycine max] (68414.m00080) | |||||
| 1320 | Not regulated | NC-NC-NC | 261534_at | At1g01820 | peroxisomal biogenesis factor 11 family protein/PEX11 family protein contains Pfam PF05648: Peroxisomal biogenesis factor 11 |
| (PEX11) (68414.m00101) | |||||
| 1321 | Not regulated | NC-NC-NC | 261535_at | At1g01725 | expressed protein (68414.m00091) |
| 1322 | Not regulated | NC-NC-NC | 261536_at | At1g01790 | K+ efflux antiporter, putative (KEA1) identical to GB: AAD01191 GI: 4101473 from [Arabidopsis thaliana]; Monovalent cation:proton |
| antiporter family 2 (CPA2 family) member, PMID: 11500563 (68414.m00098) | |||||
| 1323 | Not regulated | NC-NC-NC | 261537_at | At1g01800 | short-chain dehydrogenase/reductase (SDR) family protein similar to carbonyl reductase GI: 1049108 from [Mus musculus] |
| (68414.m00099) | |||||
| 1324 | Not regulated | NC-NC-NC | 261540_at | At1g63610 | expressed protein (68414.m07191) |
| 1325 | Not regulated | NC-NC-NC | 261530_at | At1g63460 | glutathione peroxidase, putative contains Pfam profile: PF00255 glutathione peroxidases (68414.m07176) |
| 1326 | Not regulated | NC-NC-NC | 261509_at | At1g71740 | hypothetical protein (68414.m08292) |
| 1327 | Not regulated | NC-NC-NC | 261513_at | At1g71840 | transducin family protein/WD-40 repeat family protein contains Pfam profile: PF00560 Leucine Rich Repeat (4 copies); Pfam |
| profile: PF00069 Eukaryotic protein kinase domain; Pfam profile: PF00400 WD domain, G-beta repeat (7 copies) (68414.m08302) | |||||
| 1328 | Not regulated | NC-NC-NC | 261515_at | At1g71800 | cleavage stimulation factor, putative similar to cleavage stimulation factor 64 kilodalton subunit GB: AAD47839 GI: 5713194 from |
| [Drosophila melanogaster], SP|P33240 Cleavage stimulation factor, 64 kDa subunit {Homo sapiens}; contains InterPro entry | |||||
| IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) (68414.m08298) | |||||
| 1329 | Not regulated | NC-NC-NC | 261517_at | At1g71780 | expressed protein (68414.m08296) |
| 1330 | Not regulated | NC-NC-NC | 261519_at | At1g71810 | ABC1 family protein contains Pfam domain, PF03109: ABC1 family (68414.m08299) |
| 1331 | Not regulated | NC-NC-NC | 261522_at | At1g71710 | inositol polyphosphate 5-phosphatase, putative similar to inositol polyphosphate 5-phosphatase I [Arabidopsis thaliana] |
| GI: 10444261 (68414.m08289) | |||||
| 1332 | Not regulated | NC-NC-NC | 261523_at | At1g71860 | protein tyrosine phosphatase 1 (PTP1) identical to protein tyrosine phosphatase 1 GI: 3170531 from [Arabidopsis thaliana]; contains |
| Pfam profile: PF00102 protein-tyrosine phosphatase (68414.m08304) | |||||
| 1333 | Not regulated | NC-NC-NC | 261524_at | At1g14300 | expressed protein contains Pfam PF04063: Domain of unknown function (DUF383) and PF04064: Domain of unknown function |
| (DUF384) (68414.m01695) | |||||
| 1334 | Not regulated | NC-NC-NC | 261526_at | At1g14370 | protein kinase (APK2a) identical to protein kinase APK2a GI: 2852447 from [Arabidopsis thaliana] (68414.m01703) |
| 1335 | Not regulated | NC-NC-NC | 261481_at | At1g14260 | zinc finger (C3HC4-type RING finger) family protein contains Pfam domain, PF00097: Zinc finger, C3HC4 type (RING finger) |
| (68414.m01689) | |||||
| 1336 | Not regulated | NC-NC-NC | 261483_at | At1g14270 | CAAX amino terminal protease family protein contains Pfam profile PF02517: CAAX amino terminal protease family |
| (68414.m01691) | |||||
| 1337 | Not regulated | NC-NC-NC | 261486_at | At1g14510 | PHD finger family protein contains Pfam domain, PF00628: PHD-finger (68414.m01720) |
| 1338 | Not regulated | NC-NC-NC | 261487_at | At1g14340 | RNA recognition motif (RRM)-containing protein (68414.m01699) |
| 1339 | Not regulated | NC-NC-NC | 261489_at | At1g14450 | expressed protein contains similarity to cytochrome c oxidase subunit I GI: 5678701 from [Loligo pealei] (68414.m01714) |
| 1340 | Not regulated | NC-NC-NC | 261490_at | At1g14320 | 60S ribosomal protein L10 (RPL10A)/Wilm's tumor suppressor protein-related similar to tumor suppressor GI: 575354 from [Oryza |
| sativa] (68414.m01697) | |||||
| 1341 | Not regulated | NC-NC-NC | 261492_at | At1g14290 | acid phosphatase, putative similar to acid phosphatase [Lupinus albus] GI: 5360721; contains Pfam profile PF01598 sterol |
| desaturase (68414.m01694) | |||||
| 1342 | Not regulated | NC-NC-NC | 261493_at | At1g14410 | DNA-binding protein-related similar to DNA-binding protein p24 GI: 9651810 from [Solanum tuberosum] (68414.m01709) |
| 1343 | Not regulated | NC-NC-NC | 261495_at | At1g28410 | expressed protein (68414.m03493) |
| 1344 | Not regulated | NC-NC-NC | 261439_at | At1g28395 | expressed protein (68414.m03489) |
| 1345 | Not regulated | NC-NC-NC | 261440_at | At1g28510 | expressed protein (68414.m03505) |
| 1346 | Not regulated | NC-NC-NC | 261408_s_at | At1g07820 | histone H4 identical to histone H4 from Lycopersicon esculentum GI: 297150, Lolium temulentum SP|P02308, Acropora formosa |
| GI: 455652, Citrus jambhiri GI: 16797797 (68414.m00847) | |||||
| 1347 | Not regulated | NC-NC-NC | 261409_at | At1g07640 | Dof-type zinc finger domain-containing protein identical to zinc finger protein OBP2 GI: 5059394 from [Arabidopsis thaliana] |
| (68414.m00819) | |||||
| 1348 | Not regulated | NC-NC-NC | 261411_at | At1g07790 | histone H2B, putative strong similarity to histone H2B Arabidopsis thaliana GI: 2407802, Gossypium hirsutum SP|O22582. |
| Lycopersicon esculentum GI: 3021489, Capsicum annum SP|O49118; contains Pfam profile PF00125 Core histone | |||||
| H2A/H2B/H3/H4 (68414.m00843) | |||||
| 1349 | Not regulated | NC-NC-NC | 261413_at | At1g07630 | protein phosphatase 2C family protein/PP2C family protein similar to protein phosphatase-2c (GI: 3608412) [Mesembryanthemum |
| crystallinum]; contains Pfam PF00481: Protein phosphatase 2C domain (68414.m00818) | |||||
| 1350 | Not regulated | NC-NC-NC | 261415_at | At1g07750 | cupin family protein similar to legumin (11S-globulin) from Ginkgo biloba [GI: 949889], 11S globulin from Avena sativa [GI: 472867] |
| (68414.m00837) | |||||
| 1351 | Not regulated | NC-NC-NC | 261417_at | At1g07700 | thioredoxin family protein low similarity to thioredoxin [Gallus gallus] GI: 212766; contains Pfam profile: PF00085 Thioredoxin |
| (68414.m00827) | |||||
| 1352 | Not regulated | NC-NC-NC | 261418_at | At1g07830 | ribosomal protein L29 family protein similar to GB: CAA83057 from [Saccharomyces cerevisiae] (68414.m00849) |
| 1353 | Not regulated | NC-NC-NC | 261420_at | At1g07720 | beta-ketoacyl-CoA synthase family protein similar to GB: AAC99312 from [Arabidopsis thaliana] (Plant J. (1999) in press) |
| (68414.m00832) | |||||
| 1354 | Not regulated | NC-NC-NC | 261422_at | At1g18730 | expressed protein (68414.m02336) |
| 1355 | Not regulated | NC-NC-NC | 261424_at | At1g18700 | DNAJ heat shock N-terminal domain-containing protein low similarity to SP|Q9QYI8 DnaJ homolog subfamily B member 7 Mus |
| musculus; contains Pfam profile PF00226 DnaJ domain (68414.m02333) | |||||
| 1356 | Not regulated | NC-NC-NC | 261425_at | At1g18880 | proton-dependent oligopeptide transport (POT) family protein contains Pfam profile: PF00854 POT family (68414.m02350) |
| 1357 | Not regulated | NC-NC-NC | 261405_at | At1g18740 | expressed protein (68414.m02337) |
| 1358 | Not regulated | NC-NC-NC | 261406_at | At1g18800 | nucleosome assembly protein (NAP) family protein similar to SP|Q01105|SET protein (HLA-DR associated protein II) (PHAPII) |
| (Phosphatase 2A inhibitor I2PP2A) {Homo sapiens}; contains Pfam profile PF00956: Nucleosome assembly protein (NAP) | |||||
| (68414.m02343) | |||||
| 1359 | Not regulated | NC-NC-NC | 261376_at | At1g18660 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (68414.m02326) |
| 1360 | Not regulated | NC-NC-NC | 261377_at | At1g18850 | expressed protein (68414.m02347) |
| 1361 | Not regulated | NC-NC-NC | 261378_at | At1g18890 | calcium-dependent protein kinase 1 (CDPK1) identical to calcium-dependent protein kinase [Arabidopsis thaliana] |
| gi|604880|dbj|BAA04829; contains protein kinase domain, Pfam: PF00069; contains EF hand domain (calcium-binding EF-hand), | |||||
| Pfam: PF00036, INTERPRO: IPR002048 (68414.m02351) | |||||
| 1362 | Not regulated | NC-NC-NC | 261379_at | At1g18720 | expressed protein similar to YGL010w-like protein GI: 2982301 from [Picea mariana] (68414.m02335) |
| 1363 | Not regulated | NC-NC-NC | 261386_at | At1g05430 | expressed protein (68414.m00551) |
| 1364 | Not regulated | NC-NC-NC | 261397_at | At1g79820 | hexose transporter, putative similar to hexose transporter GI: 8347246 from (Solanum tuberosum); contains Pfam profile PF00083: |
| major facilitator superfamily protein (68414.m09322) | |||||
| 1365 | Not regulated | NC-NC-NC | 261403_at | At1g79590 | syntaxin 52 (SYP52) identical to Swiss-Prot: Q94KK7 syntaxin 52 (AtSYP52) [Arabidopsis thaliana] (68414.m09280) |
| 1366 | Not regulated | NC-NC-NC | 261344_at | At1g79710 | integral membrane transporter family protein similar to high affinity folic acid/methotrexate transporter 5 (GI: 21898554) [Leishmania |
| tarentolae]; Interpro IPR001991/PR00173 Sodium:dicarboxylater symporter family (68414.m09296) | |||||
| 1367 | Not regulated | NC-NC-NC | 261346_at | At1g79720 | aspartyl protease family protein contains Pfam domain, PF00026: eukaryotic aspartyl protease (68414.m09298) |
| 1368 | Not regulated | NC-NC-NC | 261348_at | At1g79810 | Pex2/Pex12 N-terminal domain-containing protein/zinc finger (C3HC4-type RING finger) family protein contains Pfam profiles |
| PF00097: zinc finger C3HC4 type (RING finger), PF04757: Pex2/Pex12 amino terminal region (68414.m09318) | |||||
| 1369 | Not regulated | NC-NC-NC | 261351_at | At1g79790 | haloacid dehalogenase-like hydrolase family protein contains InterPro accession IPR005834: Haloacid dehalogenase-like |
| hydrolase (68414.m09312) | |||||
| 1370 | Not regulated | NC-NC-NC | 261352_at | At1g79650 | DNA repair protein RAD23, putative similar to DNA repair by nucleotide excision (NER) RAD23 protein, isoform II GI: 1914685 from |
| [Daucus carota]; contains Pfam profiles PF00240: Ubiquitin family, PF00627: UBA/TS-N domain (68414.m09287) | |||||
| 1371 | Not regulated | NC-NC-NC | 261354_at | At1g79690 | MutT/nudix family protein contains Pfam NUDIX domain [PF00293]; very low similarity to Chain A and Chain B of Escherichia coli |
| isopentenyl diphosphate:dimethylallyl diphosphate isomerase [gi: 15826361] [gi: 15826360] (68414.m09294) | |||||
| 1372 | Not regulated | NC-NC-NC | 261362_s_at | At1g41880 | 60S ribosomal protein L35a (RPL35aB) identical to GB: CAB81600 from [Arabidopsis thaliana] (68414.m04836) |
| 1373 | Not regulated | NC-NC-NC | 261373_at | At1g53000 | cytidylyltransferase family contains Pfam profile: PF02348 cytidylyltransferase (68414.m05999) |
| 1374 | Not regulated | NC-NC-NC | 261314_at | At1g52980 | GTP-binding family protein contains Pfam domain, PF01926: GTPase of unknown function (68414.m05995) |
| 1375 | Not regulated | NC-NC-NC | 261315_at | At1g53170 | ethylene-responsive element-binding factor 8/ERF transcription factor 8 (ERF8) identical to ERF transcription factor 8 |
| GI: 10567108 from [Arabidopsis thaliana] (68414.m06025) | |||||
| 1376 | Not regulated | NC-NC-NC | 261320_at | At1g53120 | RNA-binding S4 domain-containing protein (68414.m06015) |
| 1377 | Not regulated | NC-NC-NC | 261322_at | At1g44750 | purine permease family protein similar to purine permease [Arabidopsis thaliana] GI: 7620007; contains Pfam profile PF03151: |
| Domain of unknown function, DUF250 (68414.m05126) | |||||
| 1378 | Not regulated | NC-NC-NC | 261323_at | At1g44760 | universal stress protein (USP) family protein contains Pfam profile PF00582: universal stress protein family (68414.m05128) |
| 1379 | Not regulated | NC-NC-NC | 261324_at | At1g44770 | expressed protein (68414.m05129) |
| 1380 | Not regulated | NC-NC-NC | 261326_s_at | At1g44180 | aminoacylase, putative/N-acyl-L-amino-acid amidohydrolase, putative similar to aminoacylase-1 (N-acyl-L-amino-acid |
| amidohydrolase, ACY-1) [Homo sapiens] SWISS-PROT: Q03154 (68414.m05103) | |||||
| 1381 | Not regulated | NC-NC-NC | 261328_at | At1g44835 | YbaK/prolyl-tRNA synthetase family protein contains Pfam PF04073: YbaK/prolyl-tRNA synthetases associated domain; similar to |
| 25.7 kDa protein (GI: 7271117) [Cicer arietinum] (68414.m05136) | |||||
| 1382 | Not regulated | NC-NC-NC | 261334_at | At1g44960 | expressed protein (68414.m05153) |
| 1383 | Not regulated | NC-NC-NC | 261338_at | At1g44920 | expressed protein (68414.m05147) |
| 1384 | Not regulated | NC-NC-NC | 261293_at | At1g36980 | expressed protein (68414.m04610) |
| 1385 | Not regulated | NC-NC-NC | 261294_at | At1g48430 | dihydroxyacetone kinase family protein similar to dihydroxyacetone kinases; contains Pfam profiles PF02733: DAK1 domain, |
| PF02734: DAK2 domain (68414.m05414) | |||||
| 1386 | Not regulated | NC-NC-NC | 261295_at | At1g48450 | expressed protein (68414.m05416) |
| 1387 | Not regulated | NC-NC-NC | 261299_at | At1g48550 | vacuolar protein sorting-associated protein 26 family protein/VPS26 family protein contains Pfam profile PF03643: Vacuolar |
| protein sorting-associated protein 26 (68414.m05429) | |||||
| 1388 | Not regulated | NC-NC-NC | 261301_at | At1g48570 | zinc finger (Ran-binding) family protein contains Pfam domain, PF00641: Zn-finger in Ran binding protein and others |
| (68414.m05431) | |||||
| 1389 | Not regulated | NC-NC-NC | 261304_at | At1g48440 | expressed protein (68414.m05415) |
| 1390 | Not regulated | NC-NC-NC | 261308_at | At1g48480 | leucine-rich repeat transmembrane protein kinase, putative contains similarity to many predicted protein kinases (68414.m05419) |
| 1391 | Not regulated | NC-NC-NC | 261309_at | At1g48600 | phosphoethanolamine N-methyltransferase 2, putative (NMT2) vary similar to [PEM2_ARATH Putative phosphoethanolamine N- |
| methyltransferase 2 (EC 2.1.1.103) (SP: Q944H0){Arabidopsis thaliana}; very similar to Halotolerance protein Hal3b | |||||
| (SP: P94063)[Arabidopsis thaliana]; to similar to GB: AAF61950 from [Spinacia oleracea] (68414.m05434) | |||||
| 1392 | Not regulated | NC-NC-NC | 261279_at | At1g05850 | chitinase-like protein 1 (CTL1) similar to class I chitinase GI: 7798856 from [Halimolobos perplexa var. perplexa]; contains Pfam |
| profile PF00182: Chitinase class I; identical to cDNA chitinase-like protein 1 (CTL1) CTL1-ELP1 allele GI: 17226328 | |||||
| (68414.m00612) | |||||
| 1393 | Not regulated | NC-NC-NC | 261252_at | At1g05810 | Ras-related protein (ARA-1) (ARA)/small GTP-binding protein, putative nearly identical to SP: P19892 Ras-related protein ARA-1 |
| [Arabidopsis thaliana] (Gene 76: 313-319(1989)) (68414.m00608) | |||||
| 1394 | Not regulated | NC-NC-NC | 261253_at | At1g05840 | aspartyl protease family protein contains Pfam PF00026: Eukaryotic aspartyl protease (68414.m00611) |
| 1395 | Not regulated | NC-NC-NC | 261254_at | At1g05805 | basic helix-loop-helix (bHLH) family protein (68414.m00607) |
| 1396 | Not regulated | NC-NC-NC | 261267_at | At1g26750 | expressed protein (68414.m03257) |
| 1397 | Not regulated | NC-NC-NC | 261270_at | At1g26630 | eukaryotic translation initiation factor 5A, putative/eIF-5A, putative strong similariy to SP|Q9AXQ6 Eukaryotic translation initiation |
| factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} (68414.m03243) | |||||
| 1398 | Not regulated | NC-NC-NC | 261220_at | At1g19970 | ER lumen protein retaining receptor family protein similar to SP|P33946 ER lumen protein retaining receptor 1 (KDEL receptor 1) |
| {Bos taurus}; contains Pfam profile PF00810: ER lumen protein retaining receptor (68414.m02502) | |||||
| 1399 | Not regulated | NC-NC-NC | 261225_at | At1g20100 | expressed protein (68414.m02515) |
| 1400 | Not regulated | NC-NC-NC | 261227_at | At1g20200 | 26S proteasome regulatory subunit S3, putative (RPN3) similar to SP: Q06384 from [Daucus carota] (68414.m02524) |
| 1401 | Not regulated | NC-NC-NC | 261238_at | At1g32810 | expressed protein (68414.m04044) |
| 1402 | Not regulated | NC-NC-NC | 261190_at | At1g32990 | ribosomal protein L11 family protein similar to chloroplast ribosomal protein L11 GI: 21312 from [Spinacia oleracea] |
| (68414.m04063) | |||||
| 1403 | Not regulated | NC-NC-NC | 261196_at | At1g12860 | basic helix-loop-helix (bHLH) family protein/F-box family protein contains Pfam profiles: PF00646 F-box domain, PF00010 helix- |
| loop-helix DNA-binding domain (68414.m01494) | |||||
| 1404 | Not regulated | NC-NC-NC | 261197_at | At1g12900 | glyceraldehyde 3-phosphate dehydrogenase, chloroplast, putative/NADP-dependent glyceraldehydephosphate dehydrogenase, |
| putative similar to SP|P25856 Glyceraldehyde 3-phosphate dehydrogenase A, chloroplast precursor (EC 1.2.1.13) (NADP- | |||||
| dependent glyceraldehydephosphate dehydrogenase subunit A) {Arabidopsis thaliana}; contains Pfam profiles PF02800: | |||||
| Glyceraldehyde 3-phosphate dehydrogenase C-terminal domain. PF00044: Glyceraldehyde 3-phosphate dehydrogenase NAD | |||||
| binding domain (68414.m01498) | |||||
| 1405 | Not regulated | NC-NC-NC | 261202_at | At1g12910 | flower pigmentation protein (AN11) contains 3 WD-40 repeats (PF00400); identical to GB: AAC18912 from [Arabidopsis thaliana] |
| (Genes Dev. 11 (11), 1422-1434 (1997)) (68414.m01499) | |||||
| 1406 | Not regulated | NC-NC-NC | 261203_at | At1g12845 | expressed protein (68414.m01492) |
| 1407 | Not regulated | NC-NC-NC | 261204_s_at | At3g26618 | eukaryotic release factor 1 family protein/eRF1 family protein contains Pfam profiles: PF03463 eRF1 domain 1, PF03464 eRF1 |
| domain 2, PF03465 eRF1 domain 3 (68416.m03325) | |||||
| 1408 | Not regulated | NC-NC-NC | 261206_at | At1g12800 | S1 RNA-binding domain-containing protein contains Pfam domain, PF00575: S1 RNA binding domain (68414.m01486) |
| 1409 | Not regulated | NC-NC-NC | 261207_at | At1g12830 | expressed protein (68414.m01490) |
| 1410 | Not regulated | NC-NC-NC | 261209_at | At1g12810 | proline-rich family protein contains proline rich extensin domains, INTERPRO: IPR002965 (68414.m01488) |
| 1411 | Not regulated | NC-NC-NC | 261210_at | At1g12840 | vacuolar ATP synthase subunit C (VATC)/V-ATPase C subunit/vacuolar proton pump C subunit (DET3) identical to vacuolar ATP |
| synthase subunit C SP: Q9SDS7 from [Arabidopsis thaliana] (68414.m01491) | |||||
| 1412 | Not regulated | NC-NC-NC | 261183_at | At1g34550 | expressed protein contains Pfam profile PF04765: Protein of unknown function (DUF616); expression supported by MPSS |
| (68414.m04294) | |||||
| 1413 | Not regulated | NC-NC-NC | 261165_at | At1g34430 | dihydrolipoamide S-acetyltransferase, putative similar to dihydrolipoamide S-acetyltransferase (LTA2) [Arabidopsis thaliana] |
| GI: 5881963; contains Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin- | |||||
| requiring enzyme, PF02817: e3 binding domain (68414.m04277) | |||||
| 1414 | Not regulated | NC-NC-NC | 261173_at | At1g04830 | RabGAP/TBC domain-containing protein low similarity to SP|Q08484 GTPase-activating protein GYP1 {Saccharomyces |
| cerevisiae}; contains Pfam profile PF00566: TBC domain (68414.m00479) | |||||
| 1415 | Not regulated | NC-NC-NC | 261176_at | At1g04780 | ankyrin repeat family protein contains Pfam PF00023: Ankyrin repeat (68414.m00474) |
| 1416 | Not regulated | NC-NC-NC | 261177_at | At1g04770 | male stenlity MS5 family protein similar to male sterility MS5 [Arabidopsis thaliana] GI: 3859112; contains Pfam profile PF00515 |
| TPR Domain (68414.m00473) | |||||
| 1417 | Not regulated | NC-NC-NC | 261153_at | At1g04850 | ubiquitin-associated (UBA)/TS-N domain-containing protein weak similarity to SP|P45974 Ubiquitin carboxyl-terminal hydrolase 5 |
| (EC 3.1.2.15) {Homo sapiens}; contains Pfam profile PF00627 UBA/TS-N domain (68414.m00481) | |||||
| 1418 | Not regulated | NC-NC-NC | 261154_at | At1g04970 | lipid-binding serum glycoprotein family protein low similarity to SP|P17213 Bactericidal permeability-increasing protein precursor |
| (BPI) {Homo sapiens}; contains Pfam profile PF02886: LBP/BPI/CETP family, C-terminal domain (68414.m00495) | |||||
| 1419 | Not regulated | NC-NC-NC | 261155_at | At1g04960 | expressed protein (68414.m00494) |
| 1420 | Not regulated | NC-NC-NC | 261126_at | At1g04950 | TATA box-binding protein-associated factor (TAF) family protein contains Pfam profile: PF02969 TATA box binding protein |
| associated factor (68414.m00492) | |||||
| 1421 | Not regulated | NC-NC-NC | 261128_at | At1g04860 | ubiquitin-specific protease 2 (UBP2) identical to GI: 11993463 (68414.m00482) |
| 1422 | Not regulated | NC-NC-NC | 261130_at | At1g04870 | protein arginine N-methyltransferase family protein similar to SP|Q96LAB Protein arginine N-methyltransferase 6 (EC 2.1.1.—) |
| {Homo sapiens} (68414.m00483) | |||||
| 1423 | Not regulated | NC-NC-NC | 261132_at | At1g19800 | expressed protein contains Pfam PF02405: Domain of unknown function DUF140; similar to TOLUENE TOLERANCE PROTEIN |
| TTG2B {Brucella melitensis 16M} (GI: 17982923) (68414.m02477) | |||||
| 1424 | Not regulated | NC-NC-NC | 261136_at | At1g19600 | pfkB-type carbohydrate kinase family protein contains Pfam profile: PF00294 pfkB family carbohydrate kinase (68414.m02441) |
| 1425 | Not regulated | NC-NC-NC | 261149_s_at | At1g19570 | dehydroascorbate reductase, putative similar to GB: BAA90672 from (Oryza sativa) (68414.m02437) |
| 1426 | Not regulated | NC-NC-NC | 261105_at | At1g63000 | expressed protein (68414.m07114) |
| 1427 | Not regulated | NC-NC-NC | 261107_at | At1g63010 | SPX (SYG1/Pho81/XPR1) domain-containing protein contains Pfam profile PF03105: SPX domain (68414.m07115) |
| 1428 | Not regulated | NC-NC-NC | 261110_at | At1g75440 | ubiquitin-conjugating enzyme 16 (UBC16) E2; identical to gi: 2801444, GB: AAC39325 from [Arabidopsis thaliana] (Plant Mol. Biol. |
| 23 (2), 387-396 (1993)) (68414.m08763) | |||||
| 1429 | Not regulated | NC-NC-NC | 261119_at | At1g75350 | ribosomal protein L31 family protein similar to SP: O46917 from [Guillardia theta] (68414.m08752) |
| 1430 | Not regulated | NC-NC-NC | 261120_at | At1g75410 | BEL1-like homeodomain 3 protein (BLH3) identical to BEL1-like homeodomain 3 (GI: 13877515) [Arabidopsis thaliana] |
| (68414.m08760) | |||||
| 1431 | Not regulated | NC-NC-NC | 261122_at | At1g75330 | ornithine carbamoyltransferase, chloroplast/ornithine transcarbamylase/OTCase (OTC) identical to SP|O50039 Ornithine |
| carbamoyltransferase, chloroplast precursor (EC 2.1.3.3) (OTCase) (Ornithine transcarbamylase) {Arabidopsis thaliana} | |||||
| (68414.m08750) | |||||
| 1432 | Not regulated | NC-NC-NC | 261089_at | At1g07570 | protein kinase (APK1a) identical to Protein kinase APK1A from [Arabidopsis thaliana] SWISS-PROT: Q06548 (68414.m00610) |
| 1433 | Not regulated | NC-NC-NC | 261064_at | At1g07510 | FtsH protease, putative similar to AAA-metalloprotease FtsH [Pisum sativum] GI: 15021761; contains Pfam profiles PF01434: |
| Peptidase family M41, PF00004: ATPase AAA family (68414.m00804) | |||||
| 1434 | Not regulated | NC-NC-NC | 261070_at | At1g07390 | leucine-rich repeat family protein contains leucine rid-repeat (LRR) domains Pfam: PF00560, INTERPRO: IPR001611; contains |
| similarity to Hcr2-5D [Lycopersicon esculentum] gi|3894393|gb|AAC78596 (68414.m00786) | |||||
| 1435 | Not regulated | NC-NC-NC | 261075_at | At1g07280 | expressed protein (68414.m00774) |
| 1436 | Not regulated | NC-NC-NC | 261078_at | At1g07320 | 50S ribosomal protein L4, chloroplast (CL4) identical to SP: O50061 from [Arabidopsis thaliana] (68414.m00779) |
| 1437 | Not regulated | NC-NC-NC | 261079_s_at | At1g07470 | transcription factor IIA large subunit, putative/TFIIA large subunit, putative nearly identical to transcription factor IIA large subunit |
| GI: 2826884 from [Arabidopsis thaliana]; contains Pfam profile: PF03153 transcription factor IIA, alpha/beta subunit | |||||
| (68414.m00797) | |||||
| 1438 | Not regulated | NC-NC-NC | 261083_at | At1g07310 | C2 domain-containing protein contains similarity to shock protein SRC2 [Glycine max] gi|2055230|dbj|BAA19769; contains Pfam |
| profile PF00168: C2 domain (68414.m00778) | |||||
| 1439 | Not regulated | NC-NC-NC | 261084_at | At1g07440 | tropinone reductase, putative/tropine dehydrogenase, putative similar to tropinone reductase SP: P50165 from [Datura |
| stramonium] (68414.m00794) | |||||
| 1440 | Not regulated | NC-NC-NC | 261060_at | At1g17340 | phosphoinositide phosphatase family protein contains similarity to phosphoinositide phosphatase SAC1 [Rattus norvegicus] |
| gi|11095248|gb|AAG29810; contains Pfam domain, PF02383: Sacl homology domain; identical to cDNA SAC domain protein 5 | |||||
| (SAC5) GI: 31415726 (68414.m02113) | |||||
| 1441 | Not regulated | NC-NC-NC | 261031_at | At1g17360 | COP1-interacting protein-related similar to COP1-Interacting Protein 7 (CIP7) (GI: 3327870) [Arabidopsis thaliana] (68414.m02116) |
| 1442 | Not regulated | NC-NC-NC | 261036_at | At1g17470 | developmentally regulated GTP-binding protein (DRG1) identical to developmentally regulated GTP binding protein (DRG1) |
| [Arabidopsis thaliana] GI: 2345150 (68414.m02143) | |||||
| 1443 | Not regulated | NC-NC-NC | 261038_at | At1g17490 | expressed protein (68414.m02147) |
| 1444 | Not regulated | NC-NC-NC | 261040_at | At1g17370 | oligouridylate-binding protein, putative similar to oligouridylate binding protein [Nicotiana plumbaginifolia] GI: 6996560; contains |
| InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) (68414.m02118) | |||||
| 1445 | Not regulated | NC-NC-NC | 261048_at | At1g01420 | UDP-glucoronosyt/UDP-glucosyl transferase family protein contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl |
| transferase (68414.m00057) | |||||
| 1446 | Not regulated | NC-NC-NC | 261026_at | At1g01240 | expressed protein (68414.m00039) |
| 1447 | Not regulated | NC-NC-NC | 261027_at | At1g01340 | cyclic nucleotide-regulated ion channel (CNGC10) (ACBK1) almost identical to CaM-regulated potassium ion channel (ACBK1) |
| GI: 8515883 from [Arabidopsis thaliana]; contains Pfam domain, PF00520: Ion transport protein (68414.m00049) | |||||
| 1448 | Not regulated | NC-NC-NC | 261017_at | At1g26570 | UDP-glucose 6-dehydrogenase, putative strong similarity to SP|Q96558 UDP-glucose 6-dehydrogenase (EC 1.1.1.22) (UDP-Glc |
| dehydrogenase) (UDP-GlcDH) (UDPGDH) {Glycine max}; contains Pfam profiles PF03721: UDP-glucase/GDP-mannose | |||||
| dehydrogenase family NAD binding domain, PF00984: UDP-glucose/GDP-mannose dehydrogenase family central domain, | |||||
| PF03720: UDP-glucose/GDP-mannose dehydrogenase family UDP binding domain (68414.m03237) | |||||
| 1449 | Not regulated | NC-NC-NC | 261018_at | At1g26550 | peptidyl-prolyl cis-trans isomerase PPIC-type family protein similar to SP|Q9Y237 Peptidyl-prolyl cis-trans isomerase NIMA- |
| interacting 4 (EC 5.2.1.8) (Rotamase Pin4) (PPlase Pin4) (Parvulin 14) {Homo sapiens}; contains Pfam profile PF00639: PPIC-type | |||||
| PPIASE domain (68414.m03235) | |||||
| 1450 | Not regulated | NC-NC-NC | 261023_at | At1g12200 | flavin-containing monooxygenase family protein/FMO family protein low similarity to FMO2 from Homo sapiens [SP|Q99518]; |
| contains Pfam profile: PF00743 Flavin-binding monooxygenase-like (68414.m01412) | |||||
| 1451 | Not regulated | NC-NC-NC | 260993_at | At1g12140 | flavin-containing monooxygenase family protein/FMO family protein similar to flavin-containing monooxygenase [Cavia porcellus] |
| GI: 191259; contains Pfam profile PF00743: Flavin-binding monooxygenase-like (68414.m01406) | |||||
| 1452 | Not regulated | NC-NC-NC | 260968_at | At1g12250 | thylakoid lumenal protein-related weak similarity to SP|O22160 Thylakoid lumenal 15 kDa protein, chloroplast precursor (p15) |
| {Arabidopsis thaliana}; contains Pfam profile PF00805: Pentapeptide repeats (8 copies) (68414.m01417) | |||||
| 1453 | Not regulated | NC-NC-NC | 260975_at | At1g53430 | leucine-rich repeat family protein/protein kinase family protein contains Pfam domains PF00560: Leucine Rich Repeat and |
| PF00069: Protein kinase domain (68414.m06056) | |||||
| 1454 | Not regulated | NC-NC-NC | 260979_at | At1g53510 | mitogen-activated protein kinase, putative/MAPK, putative (MPK18) mitogen-activated protein kinase (MAPK)(AtMPK18), |
| PMID: 12119167 (68414.m06068) | |||||
| 1455 | Not regulated | NC-NC-NC | 260981_at | At1g53460 | expressed protein (68414.m06060) |
| 1456 | Not regulated | NC-NC-NC | 260982_at | At1g53520 | chalcone-flavanone isomerase-related low similarity to GI: 499036 (Vitis vinifera) (68414.m06070) |
| 1457 | Not regulated | NC-NC-NC | 260988_at | At1g53570 | mitogen-activated protein kinase kinase kinase (MAPKKK), putative (MAP3Ka) identical to MEK kinase (MAP3Ka)[Arabidopsis |
| thaliana] gi|4204912|gb|AAD10848 (68414.m06080) | |||||
| 1458 | Not regulated | NC-NC-NC | 260964_at | At1g45050 | ubiquitin-conjugating enzyme 15 (UBC15) E2; identical to ubiquitin-conjugating enzyme 15 GI: 2801442 from [Arabidopsis thaliana] |
| (68414.m05165) | |||||
| 1459 | Not regulated | NC-NC-NC | 260938_at | At1g45170 | expressed protein contains similarity to vacuolating cytotoxin (vacA) GI: 6634155 from [Helicobacter pylori] (68414.m05179) |
| 1460 | Not regulated | NC-NC-NC | 260940_at | At1g45000 | 26S proteasome regulatory complex subunit p42D, putative similar to 26S proteasome regulatory complex subunit p42D |
| [Drosophila melanogaster] gi|6434958|gb|AAF08391 (68414.m05158) | |||||
| 1461 | Not regulated | NC-NC-NC | 260944_at | At1g45130 | beta-galactosidase, putative/lactase, putative similar to beta-galactosidase [Lycopersicon esculentum] GI: 7939619, beta- |
| galactosidase BG1 GI: 15081596 from [Vitis vinifera]; contains Pfam profile PF01301: Glycosyl hydrolases family 35 | |||||
| (68414.m05173) | |||||
| 1462 | Not regulated | NC-NC-NC | 260946_at | At1g06010 | expressed protein (68414.m00629) |
| 1463 | Not regulated | NC-NC-NC | 260949_at | At1g06110 | F-box family protein contains similarity to F-box protein FBX3 GI: 6103643 from [Homo sapiens]; similar to SKP1 interacting partner |
| 2 (SKIP2) TIGR_Ath1: At5g67250 (68414.m00640) | |||||
| 1464 | Not regulated | NC-NC-NC | 260953_at | At1g06070 | bZIP transcription factor, putative (bZIP69) similar to transcriptional activator RF2a GB: AF005492 GI: 2253277 from [Oryza sativa]; |
| contains Pfam profile PF00170: bZIP transcription factor (68414.m00636) | |||||
| 1465 | Not regulated | NC-NC-NC | 260956_at | At1g06040 | zinc finger (B-box type) family protein/salt-tolerance protein (STO) identical to SP|Q96288 Salt-tolerance protein [Arabidopsis |
| thaliana]; contains Pfam profile PF00643: B-box zinc finger (68414.m00632) | |||||
| 1466 | Not regulated | NC-NC-NC | 260958_at | At1g06060 | RanBPM-related similar to RANBPM {GI: 13194576}[Homo sapiens] (68414.m00635) |
| 1467 | Not regulated | NC-NC-NC | 260959_at | At1g06050 | expressed protein (68414.m00634) |
| 1468 | Not regulated | NC-NC-NC | 260927_at | At1g05940 | amino acid permease family protein low similarity to SP|P30823 High-affinity cationic amino acid transporter-1 (CAT-1) {Rattus |
| norvegicus}; contains Pfam profile PF00324: Amino acid permease (68414.m00623) | |||||
| 1469 | Not regulated | NC-NC-NC | 260912_at | At1g02560 | ATP-dependent Clp protease proteolytic subunit (ClpP1) identical to nClpP1 GB: BAA82065 GI: 5360579 from [Arabidopsis |
| thaliana]; contains Pfam profile PF00574: Clp protease; contains TIGRfam profile TIGR00493: ATP-dependent Clp protease, | |||||
| proteolytic subunit ClpP (68414.m00207) | |||||
| 1470 | Not regulated | NC-NC-NC | 260914_at | At1g02640 | glycosyl hydrolase family 3 protein similar to beta-xylosidase GB: Z84377 GI: 2102655 from [Aspergillus niger] (68414.m00214) |
| 1471 | Not regulated | NC-NC-NC | 260915_at | At1g02660 | lipase class 3 family protein contains Pfam profile PF01764: Lipase (68414.m00216) |
| 1472 | Not regulated | NC-NC-NC | 260916_at | At1g02475 | expressed protein (68414.m00198) |
| 1473 | Not regulated | NC-NC-NC | 260919_at | At1g21520 | expressed protein (68414.m02691) |
| 1474 | Not regulated | NC-NC-NC | 260923_at | At1g21570 | zinc finger (CCCH-type) family protein contains Pfam domain, PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and similar) |
| (68414.m02697) | |||||
| 1475 | Not regulated | NC-NC-NC | 260924_at | At1g21590 | protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain (68414.m02699) |
| 1476 | Not regulated | NC-NC-NC | 260899_at | At1g21370 | expressed protein (68414.m02673) |
| 1477 | Not regulated | NC-NC-NC | 260902_at | At1g21440 | mutase family protein similar to carboxyvinyl-carboxyphosphonate phosphorylmutase GB: O49290 from [Arabidopsis thaliana]; |
| similar to carboxyphosphonoenolpyruvate mutase (GI: 47149) [Streptomyces hygroscopicus]; contains Prosite PS00161: Isocitrate | |||||
| lyase signature (68414.m02681) | |||||
| 1478 | Not regulated | NC-NC-NC | 260872_at | At1g21350 | expressed protein (68414.m02669) |
| 1479 | Not regulated | NC-NC-NC | 260877_at | At1g21500 | expressed protein (68414.m02689) |
| 1480 | Not regulated | NC-NC-NC | 260878_at | At1g21450 | scarecrow-like transcription factor 1 (SCL1) identical to scarecrow-like 1 GB: AAF21043 GI: 6644390 from [Arabidopsis thaliana] |
| (68414.m02682) | |||||
| 1481 | Not regulated | NC-NC-NC | 260889_at | At1g29130 | |
| 1482 | Not regulated | NC-NC-NC | 260894_at | At1g29220 | transcriptional regulator family protein similar to Transcriptional regulator protein HCNGP (Swiss-Prot: Q02614) [Mus musculus] |
| (68414.m03574) | |||||
| 1483 | Not regulated | NC-NC-NC | 260897_at | At1g29330 | ER lumen protein retaining receptor (ERD2)/HDEL receptor identical to SP: P35402 ER lumen protein retaining receptor (HDEL |
| receptor) {Arabidopsis thaliana} (68414.m03585) | |||||
| 1484 | Not regulated | NC-NC-NC | 260898_at | At1g29070 | ribosomal protein L34 family protein similar to plastid ribosomal protein L34 precursor GB: AAF64157 GI: 7578860 from [Spinacia |
| oleracea] (68414.m03558) | |||||
| 1485 | Not regulated | NC-NC-NC | 260871_at | At1g29040 | expressed protein (68414.m03554) |
| 1486 | Not regulated | NC-NC-NC | 260840_at | At1g29050 | expressed protein similar to hypothetical protein GB: AAB67625 GI: 2342727 from [Arabidopsis thaliana] (68414.m03556) |
| 1487 | Not regulated | NC-NC-NC | 260842_at | At1g29150 | 26S proteasome regulatory subunit, putative (RPN6) similar to 19S proteosome subunit 9 GB: AAC34120 GI: 3450889 from |
| [Arabidopsis thaliana] (68414.m03567) | |||||
| 1488 | Not regulated | NC-NC-NC | 260843_at | At1g29060 | expressed protein (681414.m03557) |
| 1489 | Not regulated | NC-NC-NC | 260844_at | At1g29260 | peroxisomal targeting signal type 2 receptor (PEX7) identical to peroxisomal targeting signal type 2 receptor (Pex7p) (GI: 9502414) |
| [Arabidopsis thaliana]; WD-40 repeat protein family member, contains 6 WD-40 repeats (PF00400); similar to peroxismal targeting | |||||
| signal 2 receptor (PTS2R) (Peroxin-7) (PEX7)(SP: O00628) [Homo sapiens] (68414.m03578) | |||||
| 1490 | Not regulated | NC-NC-NC | 260855_at | At1g21920 | MORN (Membrane Occupation and Recognition Nexus) repeat-containing protein/phosphatidylinositol-4-phosphate 5-kinase- |
| related similar to phosphatidylinositol-phosphate 5-kinase AtPIP5K1 [Arabidopsis thaliana] GI: 3702691; contains Pfam profile | |||||
| PF02493: MORN repeat (68414.m02743) | |||||
| 1491 | Not regulated | NC-NC-NC | 260856_at | At1g21910 | AP2 domain-containing transcription factor family protein similar to TINY GB: CAA64359 GI: 1246403 from [Arabidopsis thaliana] |
| (68414.m02742) | |||||
| 1492 | Not regulated | NC-NC-NC | 260868_at | At1g43860 | expressed protein (68414.m05053) |
| 1493 | Not regulated | NC-NC-NC | 260813_at | At1g43700 | VirE2-interacting protein (VIP1) identical to VirE2-interacting protein VIP1 GB: AAF37279 GI: 7258340 from [Arabidopsis thaliana] |
| (68414.m05020) | |||||
| 1494 | Not regulated | NC-NC-NC | 260814_at | At1g43710 | serine decarboxylase identical to serine decarboxylase [Arabidopsis thaliana] GI: 15011302; contains Pfam profile PF00282: |
| Pyridoxal-dependent decarboxylase conserved domain (68414.m05021) | |||||
| 1495 | Not regulated | NC-NC-NC | 260817_at | At1g06900 | peptidase M16 family protein/insulinase family protein contains Pfam domain, PF05193: Peptidase M16 inactive domain; similar to |
| insulin-degrading enzyme (Insulysin, Inulinase, Insulin protease) [Mouse] SWISS-PROT: Q9JHR7 (68414.m00733) | |||||
| 1496 | Not regulated | NC-NC-NC | 260818_at | At1g06890 | transporter-related low similarity to SP|Q9NTN3 UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter {Homo sapiens}, |
| SP|Q95YI5 UDP-sugar transporter UST74c {Drosophila melanogaster}, SP|Q18779 UDP-sugar transporter sqv-7 {Caenorhabditis | |||||
| elegans}; contains 8 predicted transmembrane domains (68414.m00732) | |||||
| 1497 | Not regulated | NC-NC-NC | 260822_at | At1g06790 | RNA polymerase Rpb7 N-terminal domain-containing protein similar to SP|P35718 DNA-directed RNA polymerase III 25 kDa |
| polypeptide (EC 2.7.7.6) {Saccharomyces cerevisiae}; contains Pfam profile PF03876: RNA polymerase Rpb7, N-terminal domain | |||||
| (68414.m00722) | |||||
| 1498 | Not regulated | NC-NC-NC | 260832_at | At1g06780 | glycosyl transferase family 8 protein contains Pfam profile: PF01501 glycosyl transferase family 8 (68414.m00721) |
| 1499 | Not regulated | NC-NC-NC | 260835_at | At1g06700 | serine/threorine protein kinase, putative similar to Pto kinase interactor 1 (Pti1)[Lycopersicon esculentum] |
| gi|3668069|gb|AAC61805 (68414.m00712) | |||||
| 1500 | Not regulated | NC-NC-NC | 260780_at | At1g14610 | valyl-tRNA synthetase/valine-tRNA ligase (VALRS) nearly identical to SP|P93736 Valyl-tRNA synthetase (EC 6.1.1.9) (Valine- |
| tRNA ligase) (ValRS) {Arabidopsis thaliana} (68414.m01737) | |||||
| 1501 | Not regulated | NC-NC-NC | 260781_at | At1g14620 | expressed protein (68414.m01738) |
| 1502 | Not regulated | NC-NC-NC | 260782_at | At1g14570 | UBX domain-containing protein contains Pfam profiles PF00789: UBX domain, PF02809: Ubiquitin interaction motif |
| (68414.m01732) | |||||
| 1503 | Not regulated | NC-NC-NC | 260784_at | At1g06180 | myb family transcription factor identical to GB: CAA90748 GI: 1263093 from [Arabidopsis thaliana]; contains PFAM profile: PF00249 |
| (68414.m00650) | |||||
| 1504 | Not regulated | NC-NC-NC | 260785_at | At1g06200 | expressed protein (68414.m00652) |
| 1505 | Not regulated | NC-NC-NC | 260793_at | At1g06190 | expressed protein (68414.m00651) |
| 1506 | Not regulated | NC-NC-NC | 260794_at | At1g06210 | VHS domain-containing protein/GAT domain-containing protein weak similarity to SP|Q9UJY5 ADP-ribosylation factor binding |
| protein GGA1 {Homo sapiens}; contains Pfam profiles PF00790: VHS domain, PF03127: GAT domain (68414.m00653) | |||||
| 1507 | Not regulated | NC-NC-NC | 260803_at | At1g78340 | glutathione S-transferase, putative similar to glutathione transferase GI: 2853218 from [Carica papaya] (68414.m09129) |
| 1508 | Not regulated | NC-NC-NC | 260807_at | At1g78310 | VQ motif-containing protein contains PF05678: VQ motif (68414.m09126) |
| 1509 | Not regulated | NC-NC-NC | 260775_at | At1g78300 | 14-3-3 protein GF14 omega (GRF2) identical to GF14omega isoform GI: 487791 from [Arabidopsis thaliana] (68414.m09125) |
| 1510 | Not regulated | NC-NC-NC | 260747_at | At1g78420 | expressed protein (68414.m09138) |
| 1511 | Not regulated | NC-NC-NC | 260751_at | At1g49040 | stomatal cytokinesis defective/SCD1 protein (SCD1) contains Pfam PF02141: DENN (AEX-3) domain: contains Pfam PF00400: |
| WD domain, G-beta repeat (8 copies); identical to stomatal cytokinesis defective [Arabidopsis thaliana] GI: 19743728; supporting | |||||
| cDNA gi|19743727|gb|AY082605.1|; PMID 12874123 (68414.m05498) | |||||
| 1512 | Not regulated | NC-NC-NC | 260756_at | At1g48970 | eukaryotic translation initiation factor 2B family protein/eIF-2B family protein similar to guanine nucleotide exchange factor, eIF-2B, |
| delta subunit [Mus musculus] GI: 529428; contains Pfam profile PF01008: Initiation factor 2 subunit family (68414.m05489) | |||||
| 1513 | Not regulated | NC-NC-NC | 260766_at | At1g48960 | universal stress protein (USP) family protein contains Pfam PF00582: universal stress protein family; similar to hypothetical protein |
| GI: 7770340 from [Arabidopsis thaliana] (68414.m05487) | |||||
| 1514 | Not regulated | NC-NC-NC | 260767_s_at | At1g49140 | NADH-ubiquinone oxidoreductase-related similar to NADH-ubiquinone oxidoreductase 12 kDa subunit, mitochondrial precursor (EC |
| 1.6.5.3) (EC 1.6.99.3) (Complex I-12KD) (CI-12KD) (Swiss-Prot: Q03015)[Neurospora crassa] (68414.m05509) | |||||
| 1515 | Not regulated | NC-NC-NC | 260769_at | At1g49010 | myb family transcription factor contains Pfam profile: PF00249 myb-tike DNA-binding domain (68414.m05495) |
| 1516 | Not regulated | NC-NC-NC | 260772_at | At1g49050 | aspartyl protease family protein contains Pfam PF00026: Eukaryotic aspartyl protease; contains similarity to nucellin GI: 2290203 |
| from [Hordeum vulgare] (68414.m05500) | |||||
| 1517 | Not regulated | NC-NC-NC | 260714_at | At1g14980 | 10 kDa chaperonin (CPN10) identical to SP: P34893 from [Arabidopsis thaliana] (68414.m01790) |
| 1518 | Not regulated | NC-NC-NC | 260718_at | At1g48110 | expressed protein contains Pfam profile PF04146: YT521-B-like family (68414.m05359) |
| 1519 | Not regulated | NC-NC-NC | 260724_at | At1g48140 | dolichol-phosphate mannosyltransferase-related contains weak similarity to Swiss-Prot: Q9P2X0 dolichol-phosphate |
| mannosyltransferase subunit 3 (Dolichol-phosphate mannose synthase subunit 3, Dolichyl-phosphate beta-D-mannosyltransferase | |||||
| subunit 3, Mannose-P-dolichol synthase subunit 3, MPD synthase subunit 3, DPM synthase complex subunit 3, Prostin 1 [Homo | |||||
| sapiens] (68414.m05372) | |||||
| 1520 | Not regulated | NC-NC-NC | 260730_at | At1g48030 | dihydrolipoamide dehydrogenase 1, mitochondrial/lipoamide dehydrogenase 1 (MTLPD1) identical to GB: AAF34795 |
| [gi: 12704696] from [Arabidopsis thaliana] (68414.m05350) | |||||
| 1521 | Not regulated | NC-NC-NC | 260711_at | At1g17580 | myosin, putative similar to myosin GI: 433663 from (Arabidopsis thaliana) (68414.m02165) |
| 1522 | Not regulated | NC-NC-NC | 260682_at | At1g17510 | expressed protein (68414.m02151) |
| 1523 | Not regulated | NC-NC-NC | 260696_at | At1g32520 | expressed protein (68414.m04013) |
| 1524 | Not regulated | NC-NC-NC | 260700_at | At1g32260 | expressed protein identical to hypothetical protein GB: AAF81322 GI: 8920600 from [Arabidopsis thaliana] (68414.m03969) |
| 1525 | Not regulated | NC-NC-NC | 260704_at | At1g32470 | glycine cleavage system H protein, mitochondrial, putative similar to SP|P25855 Glycine cleavage system H protein 1. |
| mitochondrial precursor {Arabidopsis thaliana}; contains Pfam profile PF01597: Glycine cleavage H-protein (68414.m04007) | |||||
| 1526 | Not regulated | NC-NC-NC | 260705_at | At1g32400 | senescence-associated family protein contains Pfam profile PF03335: Tetraspanin family (68414.m03997) |
| 1527 | Not regulated | NC-NC-NC | 260707_s_at | At1g32410 | vacuolar protein sorting 55 family protein/VPS55 family protein contains Pfam domain PF04133: Vacuolar protein sorting 55 |
| (68414.m03999) | |||||
| 1528 | Not regulated | NC-NC-NC | 260709_at | At1g32500 | ATP-binding-cassette transporter, putative similar to ATP-binding-cassette transporter (ABC1) described in PMID; 11156608 |
| (68414.m04010) | |||||
| 1529 | Not regulated | NC-NC-NC | 260652_at | At1g32360 | zinc finger (CCCH-type) family protein contains Pfam domain, PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and similar) |
| (68414.m03989) | |||||
| 1530 | Not regulated | NC-NC-NC | 260653_at | At1g32440 | pyruvate kinase, putative similar to pyruvate kinase isozyme G, chloroplast precursor [Nicotiana tabacum] SWISS-PROT: Q40546 |
| (68414.m04004) | |||||
| 1531 | Not regulated | NC-NC-NC | 260665_at | At1g19360 | expressed protein (68414.m02409) |
| 1532 | Not regulated | NC-NC-NC | 260667_at | At1g19440 | very-long-chain fatty acid condensing enzyme, putative similar to GB: AAD37122 from [Arabidopsis thaliana] (68414.m02422) |
| 1533 | Not regulated | NC-NC-NC | 260671_at | At1g19310 | zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 zinc finger, C3HC4 type (RING finger) |
| (68414.m02401) | |||||
| 1534 | Not regulated | NC-NC-NC | 260676_at | At1g19450 | integral membrane protein, putative/sugar transporter family protein similar to GB: U43629 GI: 1209756 integral membrane protein |
| from [Beta vulgaris]; contains Pfam profile PF00083: major facilitator superfamily protein; contains TIGRfam TIGR00879: Sugar | |||||
| transporter (68414.m02423) | |||||
| 1535 | Not regulated | NC-NC-NC | 260619_at | At1g08110 | lactoylglutathione lyase, putative/glyoxalase I, putative similar to lactoylglutathione lyase SP: O04885 from [Brassica juncea] |
| (68414.m00888) | |||||
| 1536 | Not regulated | NC-NC-NC | 260626_at | At1g08040 | expressed protein contains Pfam profile PF05212: Protein of unknown function (DUF707) (68414.m00878) |
| 1537 | Not regulated | NC-NC-NC | 260635_at | At1g62420 | expressed protein contains Pfam profile PF04720: Protein of unknown function (DUF506) (68414.m07042) |
| 1538 | Not regulated | NC-NC-NC | 260638_at | At1g62390 | octicosapeptide/Phox/Bem1p (PB1) domain-containing protein/tetratricopeptide repeat (TPR)-containing protein contains Pfam |
| profiles PF00564: PB1 domain, PF00515: TPR Domain (68414.m07039) | |||||
| 1539 | Not regulated | NC-NC-NC | 260644_at | At1g53290 | galactosyltransferase family protein contains Pram profile: PF01762 galactosyltransferase; contains similarity to Avr9 elicitor |
| response protein GI: 4138265 from [Nicotiana tabacum] (68414.m06040) | |||||
| 1540 | Not regulated | NC-NC-NC | 260616_at | At1g53280 | DJ-1 family protein similar to DJ-1 protein [Homo sapiens] GI: 1780755; similar to DJ-1 beta (GI: 18642508) [Drosophila |
| melanogaster]; contains Pfam profile: PF01965 ThiJ/PfpI family; TIGRFAM TIGR01383: DJ-1 family protein (68414.m06038) | |||||
| 1541 | Not regulated | NC-NC-NC | 260587_at | At1g53210 | sodium/calcium exchanger family protein/calcium-binding EF hand family protein contains Pfam profiles: PF01699 sodium/calcium |
| exchanger protein, PF00036 EF hand (68414.m06031) | |||||
| 1542 | Not regulated | NC-NC-NC | 260588_at | At1g53320 | F-box family protein/tubby family protein (TULP7) similar to Tubby related protein 2 (Tubby-like protein 2) (P4-6 protein) |
| (Fragment) (SP: P46686) [Mus musculus]; similar to phosphodiesterase (GI: 467578) [Mus musculus]; similar to Tubby protein | |||||
| homolog 1. (Swiss-Prot: Q09306) [Caenorhabditis elegants] contains Pfam profile: PF01167: Tub family: contains Pfam PF00648: F- | |||||
| box domain (68414.m06043) | |||||
| 1543 | Not regulated | NC-NC-NC | 260595_at | At1g55890 | pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat (68414.m06410) |
| 1544 | Not regulated | NC-NC-NC | 260603_at | At1g55960 | expressed protein weak similarity to SP|P53808 Phosphatidylcholine transfer protein (PC-TP) {Mus musculus} (68414.m06418) |
| 1545 | Not regulated | NC-NC-NC | 260604_at | At1g55840 | SEC14 cytosolic factor (SEC14)/phosphoglyceride transfer protein similar to polyphosphoinositide binding protein SEC14 homolog |
| Ssh1p (GB: AAB94598) [Glycine max]; identified in Eur J Biochem 1998 Dec 1; 258(2): 402-10 as AtSEC14, characterized by | |||||
| functional complementation in S. cerevisiae. (68414.m06404) | |||||
| 1546 | Not regulated | NC-NC-NC | 260605_at | At2g43780 | expressed protein (68415.m05442) |
| 1547 | Not regulated | NC-NC-NC | 260606_at | At2g43770 | transducin family protein/WD-40 repeat family protein contains 7 WD-40 repeats (PF00400); similar to U5 snRNP-specific 40 kDa |
| protein (GI: 3820594) (Homo sapiens) (68415.m05441) | |||||
| 1548 | Not regulated | NC-NC-NC | 260586_at | At2g43630 | expressed protein (68415.m05423) |
| 1549 | Not regulated | NC-NC-NC | 260582_at | At2g43850 | ankyrin protein kinase, putative (APK1) similar to ankyrin-kinase [Medicago truncatula] gi|18700701|gb|AAL78674; contains Pfam |
| profile PF00069: Protein kinase domain; contains Pfam profile PF00023: Ankyrin repeat (68415.m05451) | |||||
| 1550 | Not regulated | NC-NC-NC | 260566_at | At2g43750 | cysteine synthase, chloroplast/O-acetylserine (thiol)-lyase/O-acetylserine sulfhydrylase/cpACS1 (OASB) identical to SP|P47999 |
| Cysteine synthase, chloroplast precursor (EC 4.2.99.8) (O-acetylserine sulfhydrylase) (O-acetylserine (Thiol)-lyase) (cpACS1) | |||||
| {Arabidopsis thaliana}; identical to cDNA O-acetylserine lyase (At.OAS.7-4) GI: 6983575 (68415.m05439) | |||||
| 1551 | Not regulated | NC-NC-NC | 260571_at | At2g43790 | mitogen-activated protein kinase, putative/MAPK, putative (MPK6) identical to mitogen-activated protein kinase homolog 6 |
| (AtMPK6)[Arabidopsis thaliana] SWISS-PROT: Q39028; PMID: 12119167 (68415.m05443) | |||||
| 1552 | Not regulated | NC-NC-NC | 260572_at | At2g43760 | molybdopterin biosynthesis MoaE family protein contains Pfam profile: PF02391 molybdopterin converting factor, subunit 2 |
| (68415.m05440) | |||||
| 1553 | Not regulated | NC-NC-NC | 260578_at | At2g47350 | PAPA-1-like family protein/zinc finger (HIT type) family protein contains Pfam domains, PF04795: PAPA-1-like conserved region |
| and PF04438: HIT zinc finger (68415.m05910) | |||||
| 1554 | Not regulated | NC-NC-NC | 260579_at | At2g47380 | cytochrome c oxidase subunit Vc family protein/COX5C family protein contains Pfam profile: PF05799 cytochrome c oxidase |
| subunit Vc (COX5C) (68415.m05914) | |||||
| 1555 | Not regulated | NC-NC-NC | 260525_at | At2g47250 | RNA helicase, putative similar to SP|P53131 Pre-mRNA splicing factor RNA helices. PRP43 (Helicase JA1) (Saccharomyces |
| cerevisiae); contains Pfam profiles PF04406: Helicase associated domain (HA2), PF00271: Helicase conserved C-terminal domain | |||||
| (68415.m05900) | |||||
| 1556 | Not regulated | NC-NC-NC | 260529_at | At2g47400 | CP12 domain-containing protein contains Pfam profile: PF02672 CP12 domain (68415.m05916) |
| 1557 | Not regulated | NC-NC-NC | 260532_at | At2g47330 | DEAD/DEAH box helicase, putative similar to RNA helicase [Rattus norvegicus] GI: 897915; contains Pfam profiles PF00270: |
| DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain (68415.m05908) | |||||
| 1558 | Not regulated | NC-NC-NC | 260538_at | At2g43460 | 60S ribosomal protein L38 (RPL38A) (68415.m05401) |
| 1559 | Not regulated | NC-NC-NC | 260542_at | At2g43560 | immunophilin/FKBP-type peptidyl-prolyl cis-trans isomerase family protein identical to Probable FKBP-type peptidyl-prolyl cis-trans |
| isomerase 2, chloroplast precursor (Ppiase) (Rotamase) (SP: O22870)[Arabidopsis thaliana]; contains Pfam PF00254: peptidyl- | |||||
| protyl cis-trans isomerase, FKBP-type (68415.m05412) | |||||
| 1560 | Not regulated | NC-NC-NC | 260543_at | At2g43330 | sugar transporter family protein similar to SP|Q96QE2 Proton myo-inositol co-transporter (Hmit) [Homo sapiens], SP|Q01440 |
| Membrane transporter D1 {Leishmania donovani}; contains Pfam profile PF00083: major facilitator superfamily protein | |||||
| (68415.m05388) | |||||
| 1561 | Not regulated | NC-NC-NC | 260544_at | At2g43540 | expressed protein (68415.m05410) |
| 1562 | Not regulated | NC-NC-NC | 260545_at | At2g43350 | glutathione peroxidase, putative (68415.m05390) |
| 1563 | Not regulated | NC-NC-NC | 260546_at | At2g43520 | trypsin inhibitor, putative similar to SP|P26780 Trypsin inhibitor 2 precursor (MTI-2) {Sinapis alba} (68415.m05411) |
| 1564 | Not regulated | NC-NC-NC | 260547_at | At2g43550 | trypsin inhibitor, putative similar to SP|P26780 Trypsin inhibitor 2 precursor (MTI-2) {Sinapis alba} (68415.m05411) |
| 1565 | Not regulated | NC-NC-NC | 260548_at | At2g43360 | biotin synthase (BioB) (BIO2) identical to SP|P54967 Pfam profile PF04055: radical SAM domain protein (68415.m05391) |
| 1566 | Not regulated | NC-NC-NC | 260549_at | At2g43535 | trypsin inhibitor, putative similar to SP|P26780 Trypsin inhibitor 2 precursor (MTI-2) {Sinapis alba} (68415.m05409) |
| 1567 | Not regulated | NC-NC-NC | 260550_at | At2g43420 | 3-beta hydroxysteroid dehydrogenase/isomerase family protein contains Pfam profile PF01073 3-beta hydroxysteroid |
| dehydrogenase/isomerase domain; similar to NAD(P)-dependent steroid dehydrogenase from Homo sapiens [SP|Q15738], Mus | |||||
| musculus [SP|Q9R1J0] (68415.m05396) | |||||
| 1568 | Not regulated | NC-NC-NC | 260552_at | At2g43430 | hydroxyacylglutathione hydrolase, mitochondrial/glyoxalase II (GLX2-1) identical to SP|O24495 Hydroxyacylglutathione hydrolase, |
| mitochondrial precursor (EC 3.1.2.6) (Glyoxalase II) (Glx II) {Arabidopsis thaliana} (68415.m05397) | |||||
| 1569 | Not regulated | NC-NC-NC | 260553_at | At2g41800 | expressed protein contains Pfam profile PF04862: Protein of unknown function, DUF642 (68415.m05166) |
| 1570 | Not regulated | NC-NC-NC | 260554_at | At2g41790 | peptidase M16 family protein/insulinase family protein contains Pfam domain, PF05193: Peptidase M16 inactive domain; similar to |
| insulin-degrading enzyme (Insulysin, Insulinase, Insulin protease) [Mouse] SWISS-PROT: Q9JHR7 (68415.m05165) | |||||
| 1571 | Not regulated | NC-NC-NC | 260523_at | At2g41720 | pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat (68415.m05156) |
| 1572 | Not regulated | NC-NC-NC | 260497_at | At2g41840 | 40S ribosomal protein S2 (RPS2C) (68415.m05171) |
| 1573 | Not regulated | NC-NC-NC | 260498_at | At2g41710 | ovule development protein, putative similar to ovule development protein AINTEGUMENTA (GI: 1209099) [Arabidopsis |
| thaliana]; Pfam domain (PF00847) (68415.m05154) | |||||
| 1574 | Not regulated | NC-NC-NC | 260499_at | At2g41760 | expressed protein (68415.m05162) |
| 1575 | Not regulated | NC-NC-NC | 260502_at | At1g47270 | F-box family protein/tubby family protein contains Pfam profiles: PF00646 F-box domain, PF01167 Tub family; similar to Chain A, |
| C-Terminal Domain Of Mouse Brain Tubby Protein Length(GI: 6730158) [Mus musculus]; similar to phosphodiesterase (GI: 467578) | |||||
| [Mus musculus]; similar to Tubby protein homolog (Swiss-Prot: O88808) [Rattus norvegicus] (68414.m05233) | |||||
| 1576 | Not regulated | NC-NC-NC | 260503_at | At1g47250 | 20S proteasome alpha subunit F2 (PAF2) (PRC2B) (PRS1) identical to GB: AAC32063 from [Arabidopsis thaliana] (Genetics 149 |
| (2), 677-692 (1998)); identical to cDNA proteasome subunit prc2b GI: 2511585 (68414.m05231) | |||||
| 1577 | Not regulated | NC-NC-NC | 260507_at | At1g47200 | MFP1 attachment factor, putative contains similarity to MFP1 attachment factor 1 GI: 7546725 from [Lycopersicon esculentum] |
| similar to MFP1 attachment factor 1 [Glycine max] gi|7546729|gb|AAF63659 (68414.m05223) | |||||
| 1578 | Not regulated | NC-NC-NC | 260508_at | At1g47260 | bacterial transferase hexapeptide repeat-containing protein contains Pfam profile PF00132: Bacterial transferase hexapeptide (four |
| repeats) (68414.m05232) | |||||
| 1579 | Not regulated | NC-NC-NC | 260509_at | At1g47240 | NRAMP metal ion transporter 2, putative (NRAMP2) similar to metal transporter Nramp3 [Arabidopsis thaliana] |
| gi|6468012|gb|AAF13278; member of the natural resistance-associated macrophage protein (NRAMP) metal transporter family, | |||||
| PMID: 11500563 (68414.m05230) | |||||
| 1580 | Not regulated | NC-NC-NC | 260511_at | At1g51570 | C2 domain-containing protein contains INTERPRO: IPR000008 C2 domain (68414.m05804) |
| 1581 | Not regulated | NC-NC-NC | 260517_at | At1g51420 | sucrose-phosphatase, putative similar to sucrose-phosphatase (SPP1) [Arabidopsis thaliana] GI: 11127757; contains Pfam profile |
| PF05116: Sucrose-6F-phosphate phosphohydrolase (68414.m05788) | |||||
| 1582 | Not regulated | NC-NC-NC | 260519_at | At1g51540 | ketch repeat-containing protein contains Pfam profile PF01344: Kelch motif (68414.m05801) |
| 1583 | Not regulated | NC-NC-NC | 260465_at | At1g10910 | pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat (68414.m01253) |
| 1584 | Not regulated | NC-NC-NC | 260466_at | At1g10900 | phosphatidylinositol-4-phosphate 5-kinase family protein similar to phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1 |
| [Arabidopsis thaliana] GI: 3702891; contains Pfam profiles PF01504: Phosphatidylinositol-4-phosphate 5-Kinase, PF02493: MORN | |||||
| repeat (68414.m01252) | |||||
| 1585 | Not regulated | NC-NC-NC | 260476_at | At1g11060 | expressed protein (68414.m01267) |
| 1586 | Not regulated | NC-NC-NC | 260482_at | At1g10950 | endomembrane protein 70, putative (68414.m01257) |
| 1587 | Not regulated | NC-NC-NC | 260442_at | At1g68220 | expressed protein (68414.m07793) |
| 1588 | Not regulated | NC-NC-NC | 260443_at | At1g68185 | ubiquitin-related similar to ubiquitin-like protein smt3/pmt3 SP: O13351 from [Fission yeast] (68414.m07789) |
| 1589 | Not regulated | NC-NC-NC | 260444_at | At1g68300 | universal stress protein (USP) family protein similar to ER6 protein [Lycopersicon esculentum] GI: 5669654; contains Pfam profile |
| PF00582: universal stress protein family (68414.m07802) | |||||
| 1590 | Not regulated | NC-NC-NC | 260426_at | At1g72370 | 40S ribosomal protein SA (RPSaA) identical to laminin receptor-like protein GB: U01955 [Arabidopsis thaliana]; identical to cDNA |
| laminin receptor homologue GI: 16379 (68414.m08371) | |||||
| 1591 | Not regulated | NC-NC-NC | 260428_at | At1g72340 | eukaryotic translation initiation factor 2B family protein/eIF-2B family protein similar to SP|Q64270 Translation initiation factor eIF- |
| 2B alpha subunit (Rattus norvegicus); contains Pfam profile PF01008: Initiation factor 2 subunit family (68414.m08368) | |||||
| 1592 | Not regulated | NC-NC-NC | 260403_at | At1g69810 | WRKY family transcription factor (68414.m08032) |
| 1593 | Not regulated | NC-NC-NC | 260409_at | At1g69935 | expressed protein (68414.m08048) |
| 1594 | Not regulated | NC-NC-NC | 260413_at | At1g69800 | CBS domain-containing protein low similarity to SP|Q9UGI9 5′-AMP-activated protein kinase, gamma-3 subunit (AMPK gamma-3 |
| chain) (AMPK gamma3) {Homo sapiens}; contains Pfam profile PF00571: CBS domain (68414.m08031) | |||||
| 1595 | Not regulated | NC-NC-NC | 260418_s_at | At1g69750 | cox19 family protein contains Pfam profile PF05490: Cox19 protein (68414.m08026) |
| 1596 | Not regulated | NC-NC-NC | 260419_at | At1g69730 | protein kinase family protein contains Pfam profile: PF00069 Eukaryotic protein kinase domain (68414.m08024) |
| 1597 | Not regulated | NC-NC-NC | 260421_at | At1g69640 | acid phosphatase, putative similar to GI: 5360721 from [Lupinus albus] (68414.m08012) |
| 1598 | Not regulated | NC-NC-NC | 260395_at | At1g69780 | homeobox-leucine zipper protein 13 (HB-13)/HD-ZIP transcription factor 13 identical to homeobox gene 13 protein (GP: 12325190) |
| [Arabidopsis thaliana] (68414.m08029) | |||||
| 1599 | Not regulated | NC-NC-NC | 260368_at | At1g69700 | ABA-responsive protein (HVA22c) identical to AIHVA22c [Arabidopsis thaliana] GI: 4884936 (68414.m08021) |
| 1600 | Not regulated | NC-NC-NC | 260369_at | At1g69620 | 60S ribosomal protein L34 (RPL34B) similar to SP: Q42351 from [Arabidopsis thaliana] (68414.m08008) |
| 1601 | Not regulated | NC-NC-NC | 260370_at | At1g69740 | porphobilinogen synthase, putative/delta-aminolevulinic acid dehydratase, putative similar to delta-aminolevulinic acid |
| dehydratase (Alad) GI: 493019 [SP|P43210] from Glycine max, SP|P24493 from Spinacia olerecea, SP|P30124 from Pisum sativum | |||||
| (68414.m08025) | |||||
| 1602 | Not regulated | NC-NC-NC | 260371_at | At1g69690 | TCP family transcription factor, putative similar to PCF1 (GI: 2580438) and PCF2 [(GI: 2580440) Oryza sativa] (68414.m08020) |
| 1603 | Not regulated | NC-NC-NC | 260374_at | At1g73960 | expressed protein similar to TATA binding protein associated factor (GI: 2827282) [Homo sapiens]; similar to Transcription initiation |
| factor TFIID 150 kDa subunit (TAFII-150) (TAFII150) (Swiss-Prot: Q24325) [Drosophila melanogaster] (68414.m08565) | |||||
| 1604 | Not regulated | NC-NC-NC | 260379_at | At1g73880 | UDP-glucoronosyl/UDP-glucosyl transferase family protein contains Pfam profile: PF00201 UDP-glucoronosyl and UDP-glucosyl |
| transferase (68414.m08556) | |||||
| 1605 | Not regulated | NC-NC-NC | 260383_s_at | At1g74060 | 60S ribosomal protein L6 (RPL6B) similar to 60S ribosomal protein L6 (YL 16 like) GB: CAB57309 from [Cyanophora paradoxa] |
| (68414.m08578) | |||||
| 1606 | Not regulated | NC-NC-NC | 280390_at | At1g73940 | expressed protein (68414.m08563) |
| 1607 | Not regulated | NC-NC-NC | 260391_at | At1g74020 | strictosidine synthase family protein similar to strictosidine synthase [Rauvolfia serpentina][SP|P15324]; contains strictosidine |
| synthase domain PF03088 (68414.m08572) | |||||
| 1608 | Not regulated | NC-NC-NC | 260393_at | At1g73920 | lipase family protein similar to lipase GB: CAA74737 [SP|O46108] from [Drosophila melanogaster] (68414.m08560) |
| 1609 | Not regulated | NC-NC-NC | 260336_at | At1g73990 | peptidase U7 family protein similar to protease IV GB: AAA57008 from [Escherichia coli]; contains Pfam profile PF01343: Peptidase |
| family U7 (68414.m08569) | |||||
| 1610 | Not regulated | NC-NC-NC | 260338_at | At1g69250 | nuclear transport factor 2 (NTF2) family protein/RNA recognition motif (RRM)-containing protein contains Pfam profiles PF02136: |
| Nuclear transport factor 2 (NTF2) domain, PF00076: RNA recognition motif (a.k.a. RRM, RBD, or RNP domain) (68414.m07935) | |||||
| 1611 | Not regulated | NC-NC-NC | 260339_at | At1g69220 | serine/threonine protein kinase, putative identical to serine/threonine kinase [Arabidopsis thaliana] gi|2352084|gb|AAB68776 |
| (68414.m07925) | |||||
| 1612 | Not regulated | NC-NC-NC | 260345_at | At1g69270 | leucine-rich repeat family protein/protein kinase family protein contains Pfam domains PF00560: Leucine Rich Repeat end |
| PF00069: Protein kinase domain (68414.m07941) | |||||
| 1613 | Not regulated | NC-NC-NC | 260350_at | At1g69410 | eukaryotic translation initiation factor 5A, putative/eIF-5A, putative strong similarity to eukaryotic initiation factor 5A (2) (Nicotiana |
| plumbaginifolia) GI: 19702, SP|Q9AXQ6| Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} | |||||
| (68414.m07972) | |||||
| 1614 | Not regulated | NC-NC-NC | 260352_at | At1g69295 | beta-1,3-glucanase-related low similarity to elicitor inducible beta-1,3-glucanase NtEIG-E76 [Nicotiana tabacum] GI: 11071974 |
| (68414.m07947) | |||||
| 1615 | Not regulated | NC-NC-NC | 260356_at | At1g69390 | chloroplast division protein, putative (MinE1) identical to chloroplast division protein homolog MinE1 GI: 17511220 from [Arabidopsis |
| thaliana] (68414.m07966) | |||||
| 1616 | Not regulated | NC-NC-NC | 260358_at | At1g69340 | appr-1-p processing enzyme family protein contains Pfam domain PF01661: Appr-1-p processing enzyme family (68414.m07956) |
| 1617 | Not regulated | NC-NC-NC | 260360_at | At1g69370 | chorismate mutase, putative similar to gi: 5732016 and SP|P42738; contains Pfam profile: PF01817: Chorismate mutase |
| (68414.m07962) | |||||
| 1618 | Not regulated | NC-NC-NC | 260362_at | At1g70530 | protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain (68414.m08117) |
| 1619 | Not regulated | NC-NC-NC | 260303_at | At1g70520 | protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain (68414.m08116) |
| 1620 | Not regulated | NC-NC-NC | 260304_at | At1g70480 | expressed protein contains Pfam profile PF02713: Domain of unknown function DUF220 (68414.m08109) |
| 1621 | Not regulated | NC-NC-NC | 260305_at | At1g70490 | ADP-ribosylation factor, putative nearly identical to ADP-ribosylation factor 1 GB: P36397 [Arabidopsis thaliana], ADP-ribosylation |
| factor GI: 166586 [Arabidopsis thaliana] (68414.m08110) | |||||
| 1622 | Not regulated | NC-NC-NC | 260308_at | At1g70610 | ABC transporter (TAP1) contains Pfam profile: PF00005 ABC transporters; similar to TAP1 protein (transporter of processed |
| antigen) GB: AAD53033 (Oncorhynchus mykiss); identical to cDNA transporter associated with antigen processing-like protein | |||||
| (TAP1) GI: 19335721 (68414.m08135) | |||||
| 1623 | Not regulated | NC-NC-NC | 260309_at | At1g70580 | glutamate:glyoxylate aminotransferase 2 (GGT2) identical to glutamate:glyoxylate aminotransferase 2 [Arabidopsis thaliana] |
| GI: 24461829; similar to alanine aminotransferase from Panicum miliaceum [SP|P34106], GI: 4730884 from Oryza sativa; contains | |||||
| Pfam profile PF00155: aminotransferase, classes I and II (68414.m08127) | |||||
| 1624 | Not regulated | NC-NC-NC | 260310_at | At1g70590 | F-box family protein contains Pf |