Title:
Immunomodulatory compounds
Kind Code:
A9


Abstract:
The present invention relates to novel heterocyclic compounds, to methods for their preparation, to compositions containing them, and to methods and use for clinical treatment of medical conditions which may benefit from immunomodulation, including rheumatoid arthritis, multiple sclerosis, diabetes, asthma, transplantation, systemic lupus erythematosis and psoriasis. More particularly the present invention relates to novel heterocyclic compounds, which are CD80 antagonists capable of inhibiting the interactions between CD80 and CD28.



Inventors:
Matthews, Ian Richard (Abingdon, GB)
Coulter, Thomas Stephen (Abingdon, GB)
Ghiron, Chiara (Abingdon, GB)
Brennan, Chris James (Abingdon, GB)
Uddin, Muhammed Kamal (Abingdon, GB)
Goran Pettersson, Lars Olof (Lund, SE)
Thrige, Dorthe Da Graca (Lund, SE)
Huxley, Philip (Abingdon, GB)
Application Number:
10/717519
Publication Date:
09/15/2005
Filing Date:
11/21/2003
Assignee:
ACTIVE BIOTECH AB (Lund, SE)
Primary Class:
Other Classes:
546/82, 548/359.5, 514/405
International Classes:
A61K31/4162; A61K31/4745; A61P37/02; C07D471/04; C07D491/04; C07D495/04; C07D519/00; G07D; (IPC1-7): A61K31/4745; A61K31/4162
View Patent Images:



Primary Examiner:
POWERS, FIONA
Attorney, Agent or Firm:
BUCHANAN, INGERSOLL & ROONEY PC (ALEXANDRIA, VA, US)
Claims:
1. A compound of formula (I) or a pharmaceutically veterinarily acceptable salt thereof: embedded image wherein R1 and R3 independently represent H; F; Cl; Br; −NO2; —CN; C1-C6 alkyl optionally substituted by F or Cl; or C1-C6 alkoxy optionally substituted by F; R2 represents H, or optionally substituted C1-C6 alkyl, C3-C7 cycloalkyl or optionally substituted phenyl; Y represents —O—, —S—, N-oxide, or —N(R5)— wherein R5 represents H or C1-C6 alkyl; X represents a bond or a divalent C1-C6 alkylene radical; R4 represents —C(═O)NR6R7, —NR7C(═O)R6, —NR7C(═O)OR6, —NHC(═O)NHR6 or —NHC(═S)NHR6 wherein R6 represents H, or a radical of formula -(Alk)b-Q wherein b is 0 or 1 and Alk is an optionally substituted divalent straight chain or branched C1-C12 alkylene, C2-C12 alkenylene or C2-C12 alkynylene radical which may be interrupted by one or more non-adjacent —O—, —S— or —N(R8)— radicals wherein R8 represents H or C1-C4 alkyl, C3-C4 alkenyl, C3-C4 alkynyl, or C3-C6 cycloalkyl, and Q represents H; —CF3; —OH; —SH; —NR8R8 wherein each R8 may be the same or different; an ester group; or an optionally substituted phenyl, C3-C7 cycloalkyl, C5-C7 cycloalkenyl or heterocyclic ring having from 5 to 8 ring atoms; and R7 represents H or C1-C6 alkyl; or when taken together with the atom or atoms to which they are attached R6 and R7 form an optionally substituted heterocyclic ring having from 5 to 8 ring atoms.

2. A compound as claimed in claim 1 wherein R1 is H, F, Cl, methyl or methoxy.

3. A compound as claimed in claim 1 or claim 2 wherein R2 is H, methyl, methoxy, cyclopropyl, phenyl, or fluoro-, chloro-, methyl, or methoxy-substituted phenyl.

4. A compound as claimed in any of the preceding claims wherein R3 is H, F, Cl, methyl, methoxy, or methylenedioxy.

5. A compound as claimed in any of the preceding claims wherein Y is —O—, —S—, or —N(R5)— wherein R5 represents H or methyl.

6. A compound as claimed in any of the preceding claims wherein X is a bond, or a —CH2— or —CH2CH2— radical.

7. A compound as claimed in any of the preceding claims wherein R4 represents —C(═O)NHR6, —NR7C(═O)R6, —NR7C(═O)OR6, —NHC(═O)NHR6 or —NHC(═S)NHR6 and in these R6 is H or a radical of formula -Alkb-Q wherein b is 0 or 1 and Alk is a —(CH2)n—, —CH((CH2)mCH3)(CH2)n—, —CH((CH2)mCH3)((CH2)pCH3)(CH2)n—, —(CH2)n—O—(CH2)m—, or —(CH2)n—O—(CH2)nO—(CH2)m—, radical where n is 1, 2, 3 or 4 and m and p are independently 0, 1, 2, 3 or 4, and Q represents H, —OH, —COOCH3 phenyl, cyclopropyl, cyclopentyl, cyclohexyl, pyridyl, furyl, thienyl, or oxazolyl. and R7 is H, or when taken together with the nitrogen atom to which they are attached R6 and R7 form a pyrrolidine-2-one or pyrrolidine-2,5-dione ring.

8. A compound as claimed in claim 1 wherein R1 is H, F, or Cl; R2 is H; R3 is H, F, or Cl; Y is —NH—; X is a bond; and R4 represents —C(═O)NHR6, —NR7C(═O)R6, —NR7C(═O)OR6 or —NHC(═O)NHR6 wherein: R6 is H or a radical of formula -Alkb-Q wherein b is 0 or 1 and Alk is a —(CH2)n—, —CH((CH2)mCH3)(CH2)n—, —CH((CH2)mCH3)((CH2)pCH3)(CH2)n—, —(CH2)n—O—(CH2)m—, or —(CH2)n—O—(CH2)n—O—(CH2)m—, radical where n is 1, 2, 3 or 4 and m and p are independently 0, 1, 2, 3 or 4, and Q represents H, —OH, —COOCH3 phenyl, cyclopropyl, cyclopentyl, cyclohexyl, pyridyl, furyl, thienyl, or oxazolyl. and R7 is H, or when taken together with the nitrogen atom to which they are attached R6 and R7 form a pyrrolidine-2-one or pyrrolidine-2,5-dione ring.

9. A compound as claimed in claim 1 wherein R1 is F, R2 is H or cyclopropyl, R3 is H, X is a bond, and R4 is —C(═O)NHR6, —NRHC(═O)R6, or —NHC(═O)NHR6.

10. N-(3-Dimethylamino propyl)-4-(4-cyclopropyl-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl]-benzamide, or pharmaceutically or veterinarily acceptable salt thereof.

11. A compound as claimed in any of claims 1 to 10 for use in the treatment of conditions which benefit from immunomodulation.

12. The use of a compound as claimed in any of claims 1 to 10 in the manufacture of a medicament for the treatment of conditions which benefit from immunomodulation.

13. A method of immunomodulation in humans and non-human primates, comprising administration to a subject in need of such treatment an immunomodulatory effective dose of a compound as claimed in any of claims 1 to 10.

14. A pharmaceutical or veterinary composition comprising a compound as claimed in any of claims 1 to 10 together with a pharmaceutically or veterinarily acceptable excipient or carrier.

Description:

The present invention relates to novel heterocyclic compounds, to methods for their preparation, to compositions containing them, and to methods and use for clinical treatment of medical conditions which may benefit from immunomodulation, including rheumatoid arthritis, multiple sclerosis, diabetes, asthma, transplantation, systemic lupus erythematosis and psoriasis. More particularly the present invention relates to novel heterocyclic compounds, which are CD80 antagonists capable of inhibiting the interactions between CD80 and CD28.

BACKGROUND OF THE INVENTION

The immune system possesses the ability to control the homeostasis between the activation and inactivation of lymphocytes through various regulatory mechanisms during and after an immune response. Among these are mechanisms that specifically inhibit and/or turn off an immune response. Thus, when an antigen is presented by MHC molecules to the T-cell receptor, the T-cells become properly activated only in the presence of additional co-stimulatory signals. In the absence of accessory signals there is no lymphocyte activation and either a state of functional inactivation termed anergy or tolerance is induced, or the T-cell is specifically deleted by apoptosis. One such co-stimulatory signal involves interaction of CD80 on specialised antigen-presenting cells with CD28 on T-cells, which has been demonstrated to be essential for full T-cell activation. (Lenschow et al. (1996) Annu. Rev. Immunol., 14, 233-258)

A paper by Erbe et al, in J. Biol. Chem. Vol. 277, No. 9, pp 7363-7368 (2002), describes three small molecule ligands which bind to CD80, and inhibit binding of CD80 to CD28 and CTLA4. Two of the disclosed ligands are fused pyrazolones of structures A and B: embedded image

DESCRIPTION OF THE INVENTION

According to the present invention there is provided a compound of formula (I) or a pharmaceutically or veterinarily acceptable salt thereof: embedded image
wherein

    • R1 and R3 independently represent H; F; Cl; Br; —NO2; —CN; C1-C6 alkyl optionally substituted by F or Cl; or C1-C6 alkoxy optionally substituted by F;
    • R2 represents H, or optionally substituted C1-C6 alkyl, C3-C7 cycloalkyl or optionally substituted phenyl;
    • Y represents —O—, —S—, N-oxide, or —N(R5)— wherein R5 represents H or C1-C6 alkyl;
    • X represents a bond or a divalent C1-C6 alkylene radical;
    • R4 represents —C(═O)NR6R7, —NR7C(═O)R6, —NR7C(═O)OR6, —NHC(═O)NHR6, or —NHC(═S)NHR6 wherein
    • R6 represents H, or a radical of formula -(Alk)b-Q wherein b is 0 or 1, and
    • Alk is an optionally substituted divalent straight chain or branched C1-C12 alkylene, C2-C12 alkenylene or C2-C12 alkynylene radical which may be interrupted by one or more non-adjacent —O—, —S— or —N(R8)— radicals wherein R8 represents H or C1-C4 alkyl, C3-C4 alkenyl, C3-C4 alkynyl, or C3-C6 cycloalkyl, and
    • Q represents H; —CF3; —OH; —SH; —NR8R8 wherein each R8 may be the same or different; an ester group; or an optionally substituted phenyl, C3-C7 cycloalkyl, C5-C7 cycloalkenyl or heterocyclic ring having from 5 to 8 ring atoms; and
    • R7 represents H or C1-C6 alkyl; or when taken together with the atom or atoms to which they are attached R6 and R7 form an optionally substituted heterocyclic ring having from 5 to 8 ring atoms.

Compounds of general formula (I) are CD80 antagonists. They inhibit the interaction between CD80 and CD28 and thus the activation of T cells, thereby modulating the immune response.

Accordingly the invention also includes:

(i) a compound of formula (I) or a pharmaceutically or veterinarily acceptable salt thereof for use in the treatment of conditions which benefit from immunomodulation.

(ii) the use of a compound of formula (I) or a pharmaceutically or veterinarily acceptable salt thereof in the manufacture of a medicament for the treatment of conditions which benefit from immunomodulation,.

(iii) a method of immunomodulation in humans and non-human primates, comprising administration to a subject in need of such treatment an immunomodulatory effective dose of a compound of formula (I) or a pharmaceutically or veterinarily acceptable salt thereof.

(iv) a pharmaceutical or veterinary composition comprising a compound of formula (I) or a pharmaceutically or veterinarily acceptable salt thereof together with a pharmaceutically or veterinarily acceptable excipient or carrier.

Conditions which benefit from immunomodulation include:

  • Adrenal insufficiency
  • Allergic angiitis and granulomatosis
  • Amylodosis
  • Ankylosing spondylitis
  • Asthma
  • Autoimmune Addison's disease
  • Autoimmune alopecia
  • Autoimmune chronic active hepatitis
  • Autoimmune hemolytic anemia
  • Autoimmune neutropenia
  • Autoimmune thrombocytopenic purpura
  • Autoimmune vasculitides
  • Behcet's disease
  • Cerebellar degeneration
  • Chronic active hepatitis
  • Chronic inflammatory demyelinating polyradiculoneuropathy
  • Dermatitis herpetiformis
  • Diabetes
  • Eaton-Lambert myasthenic syndrome
  • Encephalomyelitis
  • Epidermolysis bullosa
  • Erythema nodosa
  • Gluten-sensitive enteropathy
  • Goodpasture's syndrome
  • Graft versus host disease
  • Guillain-Barre syndrome
  • Hashimoto's thyroiditis
  • Hyperthyrodism
  • Idiopathic hemachromatosis
  • Idiopathic membranous glomerulonephritis
  • Minimal change renal disease
  • Mixed connective tissue disease
  • Multifocal motor neuropathy
  • Multiple sclerosis
  • Myasthenia gravis
  • Opsoclonus-myoclonus syndrome
  • Pemphigoid
  • Pemphigus
  • Pernicious anemia
  • Polyarteritis nodosa
  • Polymyositis/dermatomyositis
  • Post-infective arthritides
  • Primary biliary sclerosis
  • Psoriasis
  • Reactive arthritides
  • Reiter's disease
  • Retinopathy
  • Rheumatoid arthritis
  • Sclerosing cholangitis
  • Sjögren's syndrome
  • Stiff-man syndrome
  • Subacute thyroiditis
  • Systemic lupus erythematosis
  • Systemic sclerosis (scleroderma)
  • Temporal arteritis
  • Thromboangiitis obliterans
  • Transplantation rejection
  • Type I and type II autoimmune polyglandular syndrome
  • Ulcerative colitis
  • Uveitis
  • Wegener's granulomatosis

As used herein the term “alkylene” refers to a straight or branched alkyl chain having two unsatisfied valencies, for example —CH2—, —CH2CH2—, —CH2CH2CH2—, —CH(CH3)CH2—, —CH(CH2CH3)CH2CH2CH3, and —C(CH3)3.

As used herein the term “heteroaryl” refers to a 5- or 6-membered aromatic ring containing one or more heteroatoms. Illustrative of such groups are thienyl, furyl, pyrrolyl, imidazolyl, benzimidazolyl, thiazolyl, pyrazolyl, isoxazolyl, isothiazolyl, triazolyl, thiadiazolyl, oxadiazolyl, pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl.

As used herein the unqualified term “heterocyclyl” or “heterocyclic” includes “heteroaryl” as defined above, and in particular means a 5-8 membered aromatic or non-aromatic heterocyclic ring containing one or more heteroatoms selected from S, N and O, including for example, pyrrolyl, furanyl, thienyl, piperidinyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, thiadiazolyl, pyrazolyl, pyridinyl, pyrrolidinyl, pyrimidinyl, morpholinyl, piperazinyl, indolyl, morpholinyl, benzofuranyl, pyranyl, isoxazolyl, quinuclidinyl, aza-bicyclo[3.2.1]octanyl, benzimidazolyl, methylenedioxyphenyl, maleimido and succinimido groups.

Unless otherwise specified in the context in which it occurs, the term “substituted” as applied to any moiety herein means substituted with one or more of the following substituents, namely (C1-C6)alkyl, trifluoromethyl, (C1-C6)alkoxy (including the special case where a ring is substituted on adjacent ring C atoms by methylenedioxy or ethylenedioxy), trifluoromethoxy, (C1-C6)alkylthio, phenyl, benzyl, phenoxy, (C3-C8)cycloalkyl, hydroxy, mercapto, amino, fluoro, chloro, bromo, cyano, nitro, oxo, —COOH, —SO2OH, —CONH2, —SO2NH2, —CORA, —COORA, —SO2ORA, —NHCORA, —NHSO2RA, —CONHRA, —SO2NHRA, —NHRA, —NRARB, —CONRARB or —SO2NRARB wherein RA and RB are independently a (C1-C6)alkyl group. In the case where “substituted” means substituted by (C3-C8)cycloalkyl, phenyl, benzyl or phenoxy, the ring thereof may itself be substituted with any of the foregoing, except (C3-C8)cycloalkyl phenyl, benzyl or phenoxy.

As used herein the unqualified term “carbocyclyl” or “carbocyclic” refers to a 5-8 membered ring whose ring atoms are all carbon.

Some compounds of the invention contain one or more chiral centres because of the presence of asymmetric carbon atoms. The presence of asymmetric carbon atoms gives rise to stereoisomers or diastereoisomers with R or S stereochemistry at each chiral centre. The invention includes all such stereoisomers and diastereoisomers and mixtures thereof.

Salts of salt forming compounds of the invention include physiologically acceptable acid addition salts for example hydrochlorides, hydrobromides, sulphates, methane sulphonates, p-toluenesulphonates, phosphates, acetates, citrates, succinates, lactates, tartrates, fumarates and maleates; and base addition salts, for example sodium, potassium, magnesium, and calcium salts. Where the compound contains an amino group, quaternary amino salts are also feasable, and are included in the invention.

In the compounds of the invention the following are examples of the several structural variables:

    • R1 may be, for example, H, F, Cl, methyl, methoxy, or methylenedioxy. Currently it is preferred that R1 is H, Cl or especially F;
    • R2 may be, for example H, methyl, methoxy, cyclopropyl, phenyl, or fluoro-, chloro-, methyl, or methoxy-substituted phenyl. H or cyclopropyl is presently preferred;
    • R3 may be, for example, H, F, Cl, methyl, methoxy, or methylenedioxy. Currently it is preferred that R3 is F or Cl, and it is most preferred that R3 be H;
    • Y may be, for example, —O—, —S—, or —N(R5)— wherein R5 represents H or methyl. —NH— or —S— is presently preferred.
    • X may be, for example a bond, or a —CH2— or —CH2CH2— radical. A bond is presently preferred.
    • R4 represents —C(═O)NR6R7, —NR7C(═O)R6, —NR7C(═O)OR6, —NHC(═O)NHR6, or —NHC(═S)NHR6. Of these —NR7C(═O)R6, and especially —C(═O)NR6R7 and —NHC(═O)NHR6 are curently preferred. R7 is preferably H, but a wide range of R6 substituents have given rise to highly active compounds of the invention. Many exemplary R6 substituents appear in the compounds of the Examples below.
    • R6 may be, for example, H or a radical of formula -Alkb-Q wherein b is 0 or 1 and
    • Alk may be, for example a —(CH2)n—, —CH((CH2)mCH3)(CH2)n—, —C((CH2)mCH3)((CH2)pCH3) (CH2)n—, —(CH2)n—O—(CH2)m—, —(CH2)n—NH—(CH2)m—, or —(CH2)n—NH—(CH2)m—NH—(CH2)p— radical where n is 1, 2, 3 or 4 and m and p are independently 0, 1, 2, 3 or 4, and
    • Q may represent H, —OH, —COOCH3, phenyl, cyclopropyl, cyclopentyl, cyclohexyl, pyridyl, furyl, thienyl, or oxazolyl; and
    • R7 may be, for example, H, or when taken together with the atom or atoms to which they are attached R6 and R7 may form a heterocyclic ring of 5, 6 or 7 members.

Specific examples of R4 groups include those present in the compounds of the Examples herein.

Compounds of the invention may be prepared by synthetic methods known in the literature, from compounds which are commercially available or are accessible from commercially available compounds. For example, compounds of formula (I) wherein R4 is a group —NR7C(═O)R6 may be prepared by acylation of an amine of formula (II) with an acid chloride of formula (III): embedded image

Compounds of the invention wherein R4 is a group —NHC(═O)NHR6 may be prepared by reaction of an amine of formula (IIA) with an isocyanate of formula (IIIA) embedded image

Compounds of the invention wherein R4 is a group —C(═O)NHR6 may be prepared by reaction of an acid chloride of formula (IIB) with an amine NHR6R7: embedded image

Compounds of the invention wherein R4 is a group —NR7C(═O)OR6 may be prepared by reaction of an amine of formula (II) with a chloroformate ClC(═O)OR6.

The following Examples illustrate the preparation of compounds of the invention:

Preparation of Intermediate 1

2-(4-Nitrophenyl)-6-fluoro-2,5-dihydropyrazolo[4,3-c]-quinolin-3-one

embedded image

4-Nitrophenylhydrazine (2.28 g, 0.014 mol) was added in one portion to a stirred solution of 4-chloro-8-fluoro-quinoline-3-carboxylic acid ethyl ester (3.58 g, 0.014 mol) in anhydrous n-butyl alcohol (50 ml) at room temperature. The mixture was refluxed for 16 h under nitrogen, cooled to room temperature and then filtered to leave an orange solid. The solid was purified by washing sequentially with ethyl acetate (20 ml) and heptane (20 ml) and then finally dried under suction to give the pyrazolone (3.93 g, 87%) as a dark orange solid, LCMS m/z 325.24 [M+H]+ @ RT 1.47 min.

Preparation of Intermediate 2

2-(4-Aminophenyl)-6-fluoro-2,5-dihydropyrazolo[4,3-c]-quinolin-3-one

embedded image

Tin (II) chloride dihydrate (12.5 g, 0.055 mol) was added in one portion to a stirred solution of 2-(4-nitro-phenyl)-6-fluoro-2,5-dihydro-pyrazolo[4,3-c]quinolin-3-one (intermediate 1) (3.59 g, 0.011 mol) in ethyl alcohol (110 ml) at room temperature. The mixture was then heated to 80° C. for 8 h, cooled to room temperature and filtered to leave a yellow solid. The solid was suspended in a bi-phasic solution of ethyl acetate (1L), a saturated solution of Rochelles salt (500 ml) and a saturated solution of sodium bicarbonate (500 ml) and stirred at room temperature for 2 h. The mixture was filtered and the remaining solid was washed with water and dried under vacuum to afford the title compound (3.39 g, 99%) as a bright yellow solid, LCMS m/z 295.30 [M+H]+ @ RT 0.84 min.

EXAMPLE 1

N-[4-(6-Fluoro-3-oxo-3,5-dihydropyrazolo[4,3-c]quinolin-2-yl)-phenyl]-2-methyl-butyramide

embedded image

(±)-2-Methylbutyryl chloride (13.6 μl, 0.11 mmol) was added dropwise over 30 sec to a stirred solution of 2-(4-amino-phenyl)-6-fluoro-2,5-dihydro-pyrazolo[4,3-c]quinolin-3-one (Intermediate 2) (30 mg, 0.10 mmol), triethylamine (14 μl, 0.11 mmol) and 4-dimethylaminopyridine (2.4 mg, 0.02 mmol) in dichloromethane (1 ml) at room temperature. The mixture was stirred at room temperature for 16 h. The yellow solid was then filtered and purified by washing sequentially with a saturated solution of sodium bicarbonate (1 ml), ethyl acetate (1 ml) and ethyl alcohol (0.5 ml) and finally dried under suction to give the title compound (10 mg, 26%) as a bright yellow solid, LCMS m/z 379.36 [M+H]+ @ RT 1.18 min. δH(400 MHz, (CD3)2SO) 9.89 (1H, s), 8.52 (1H, s), 8.15 (2H, d J 9.0 Hz), 8.01 (1H, d J 7.0 Hz), 7.69 (2H, d J 9.0 Hz) 7.57-7.46 (2H, m), 2.46-2.39 (1H, m), 1.69-1.36 (2H, m), 1.11 (3H, d J 6.8 Hz), 0.91(3H, t J 7.3 Hz).

The title compound, and compounds of subsequent Examples, were tested in the assay described below in the Assay Section, to determine their activities as inhibitors of the CD80-CD28 interaction. The present title compound had an activity rating of ***.

EXAMPLES 2-49

The following compounds were synthesized by the route described in Example 1, substituting the appropriate acid chloride for (±)-2-methylbutyryl chloride:

EXAMPLE 2

2-Methyl-pentanoic acid [4-(6-fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-phenyl]-amide

embedded image

δH(400 MHz, (CD3)2SO) 9.92 (1H, s), 8.53 (1H, s) 8.12 (2H, d J 9.2 Hz), 8.05 (1H, d J 7.6 Hz), 7.70 (2H, d J 9.2 Hz), 7.63-7.53 2H, m), 1.68-1.58 (1H, m), 1.38-1.28 (3H, m), 1.11 (3H, d J 6.6 Hz), 0.91 (3H, t J 7.1 Hz).

    • Activity ***

EXAMPLE 3

1-Methyl-1H-pyrrole-2-carboxylic acid [4-(6-fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-phenyl]-amide

embedded image

δH(400 MHz, (CD3)2SO) 9.76 (1H, s), 8.50 (1H, s), 8.26 (2H, d 9.0 Hz), 7.97-7.94 (1H, m), 7.73 (2H, d J 9.0 Hz), 7.39-7.28 (2H, m), 7.07-7.01 (2H, m), 3.91 (3H, s)

    • Activity *

EXAMPLE 4

N-[4-(6-Fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-phenyl]-3-methyl-butyramide

embedded image

δH(400 MHz, (CD3)2SO) 9.92 (1H, s), 8.52 (1H, s), 8.14 (2H, d J 9.2 Hz), 8.01 (1H, d J 7.3 Hz), 7.67 (2H, d J 9.2 Hz), 7.57-7.47 (2H, m), 2.21 (2H, d J 6.8 Hz), 2.14-2.07 (1H, m), 0.96 (6H, d J 6.6 Hz).

    • Activity **

EXAMPLE 5

2-Propyl-pentanoic acid [4-(6-fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-phenyl]-amide

embedded image

δH(400 MHz, (CD3)2SO) 9.93 (1H, s), 8.53 (1H, s), 8.11 (2H, d J 9.0 Hz), 8.05 (1H, d J 7.8 Hz), 7.70 (2H, d J 9.0 Hz), 7.59-7.46 (2H, m), 2.46-2.35 (1H, m), 1.63-1.27 (4H, m), 0.90(6H, t J 7.1 Hz).

    • Activity *

EXAMPLE 6

5-[4-(6-Fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)phenylcarbamoyl]-pentanoic acid methyl ester

embedded image

δH(400 MHz, (CD3)2SO) 9.85 (1H, s), 8.47 (1H, s), 8.25 (2H, d J 9.0 Hz), 7.91-7.90 (1H, m), 7.59 (2H, d J 9.0 Hz), 7.29-7.20 (2H, m), 3.61 (3H, s), 2.38-2.28 (4H, m), 1.64-1.50 (4H, m)

    • Activity ***

EXAMPLE 7

N-[4-(6-Fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-phenyl]-2,2-dimethyl-propionamide

embedded image

δH(400 MHz, (CD3)2SO) 9.26 (1H, S), 8.52 (1H, s), 8.15 (2H, d J 9.2 Hz), 8.03 (1H, d J 8.8 Hz), 7.71 (2H, d J 9.2 Hz), 7.56-7.47 (2H, m), 1.26 (9H, s)

    • Activity **

Examples 8 to 28 were also prepared by the method of Example 1 using the appropriate acid chloride:

embedded image
M.S.
ExampleXR(MH+)Activity
86-F embedded image 443.4**
96-F—CH2Cl371.31**
106-F embedded image 389.34*
116-F embedded image 485.45*
126-FCO2Me381.34**
136-FOEt367.18
146-F embedded image 507.43*
156-F embedded image 466.41**
166-FMe337.36**
176-FCH(Et)CH2CH2CH2Me421.46*
186-FCH(Et)2393.41***
196-F embedded image 405.41**
206-F embedded image 448.44**
216-F embedded image 481.35**
226-F embedded image 423.42***
236-F(CH2)8CO2Me493.51**
246-FiPr365.36***
256-FCH2OCH2CH2OMe411.4**
266-FCH(Me) (nPr)393.42***
276-FCH2OMe367.24**
286-F embedded image 390.33**
296-FCH2CH2CH2N+(Me)3422.1 (M+)***
306-FCH2CH2CH2N(Me)2408.3***
316-FCH2NHCH2CH2CH2N(Me) (Ph)499.3*
326-F embedded image 485.3*
336-F embedded image 505.1***
346-F embedded image 517.2***
356-F embedded image 477.1***
366-F embedded image 457.1**
376-F embedded image 463.1**
386-F embedded image 438.3**
396-F embedded image 463.2***
406-F embedded image 460.4**
416-FCH2NHCH2CH2N(iPr)2479.4**
426-F embedded image 420.2**
43HCH(NH2)CH3348.3**
44HCH(Me)nPr375.3*
45HiPr347.3**
466-FCH(NH2)CH3366.3***
47HCH(Me)Et361.3**
486-F embedded image 529.1**
496-FCH2N(Me)CH2Ph456.4**

Preparation of Intermediate 3

3-(6-Fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-benzoic acid

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3-Hydrazinobenzoic acid (1.91 g, 0.013 mol) was added in one portion to a stirred solution of 4-chloro-8-fluoro-quinoline-3-carboxylic acid ethyl ester (2.93 g, 0.011 mol) in n-butanol (60 ml) at room temperature. The solution was heated to reflux for 16 h, cooled to room temperature and the resulting yellow solid filtered, washed with tert-butyl methyl ether and then dried. The solid was redissolved in a solution of tetrahydrofuran:water (2:1; 21 ml) and lithium hydroxide (1.27 g, 0.031 mol) was then added. After stirring at room temperature for 16 h, concentrated hydrochloric acid (3 ml) was added dropwise to the mixture to precipitate a yellow solid which was filtered and dried under vacuum to give the title compound (intermediate 3) (2.32 g, 63%) as a bright yellow solid.

Preparation of Intermediate 4

3-(6-Fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-benzoyl chloride

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Oxalyl chloride (20 ml, 0.2 mol) was added dropwise over 2 min to a stirred solution of 3-(6-fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-benzoic acid (intermediate 3) (2.0 g, 6.1 mmol) in dichloromethane (10 ml) at room temperature. N,N-Dimethylformamide (50 μl) was then added and the resulting mixture heated to 50° C. for 1 h. The solution was then cooled to room temperature and then concentrated in vacuo to leave the title compound (intermediate 4) (2.0 g, 96%) as a beige solid.

EXAMPLE 50

3-(6-Fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-N-(3-methoxy-propyl)-benzamide

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3-Methoxypropylamine (0.026 g, 0.29 mmol) was added to a stirred solution of 3-(6-fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-benzoyl chloride (intermediate 4) (26 mg 0.29 mmol) in tetrahydrofuran (2 ml) and the mixture stirred at room temperature for 15 min. Triethylamine (0.2 ml, 1.4 mmol) was then added and the resulting mixture stirred overnight. 1 M Hydrochloric acid (3-4 ml) was added dropwise to precipitate a yellow solid which was filtered and dried under suction to give the amide (79 mg, 0.20 mmol) as a yellow solid, LCMS m/z 395.25 [M+H]+ @ RT 1.04 min; δH(400 MHz, (CD3)2SO) 8.59 (1H, m), 8.57 (1H, s), 8.39 (1H, app d J 9.3 Hz), 8.08 (1H, app d J 7.3 Hz), 7.66-7.53 (5H, m), 3.37-3.33 (4H, m), 3.27 (3H, s), 1.83-1.77 (2H, m).

    • Activity **

EXAMPLE 51

N-Ethyl-3-(6-fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]-quinolin-2-yl)-benzamide

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Prepared by the method of Example 53 substituting ethylamine for 3-methoxypropylamine.

δH(400 MHz, (CD3)2SO) major rotomer quoted; 8.56 (1H, br s), 8.47 (1H, m), 8.21 (2H, d J 8.5 Hz), 7.94 (2H, d J 8.5 Hz), 3.96 (3H, s), 3.31 (2H, q J 7.3 Hz), 2.58 (3H, s), 1.15 (3H, t J 7.4 Hz).

    • Activity **

EXAMPLE 52

N-Benzyl-3-(6-fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]-quinolin-2-yl)-benzamide

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Prepared by the method of Example 53 substituting benzylamine for 3-methoxypropylamine.

LCMS m/z 427.16 [M+H]+ @ RT 1.28 min.

    • Activity *

Examples 53 to 64 were prepared by the method of example 50, using the appropriate amine.

embedded image
M.S.
ExampleXRR′(MH+)Activity
536-FCH2CH2CH2N(Me)2Me422.5*
546-FCH2CH2l CH2N(Me)2H408.4**
556-F embedded image H420.4*
566-F embedded image H434.4*
576-F embedded image H448.4**
586-FCH2CH2CH2CH2N(Me)2H422.4**
596-FCH2CH2OMeH381.3**
606-FEtEt379.3*
616-FCH2CO2MeH395.2*
626-FCH2CCHH361.3**
636-FCH2PhMe427.2**
646-F embedded image 463.3*

EXAMPLE 65

N-(3-Dimethylamino propyl)-4-(4-cyclopropyl-3-oxo-3,5-dihydro-pyrazolo[4,3-c)quinolin-2-yl]-benzamide

Step 1

2-cyclopropyl-4-oxo-1,4-dihydro-quinoline-3-carboxylic acid ethyl ester

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A solution of 3-cyclopropyl-3-oxo-propionic acid methyl ester (6.2 g, 0.038 mols), 2-amino benzoic acid ethyl ester (4.95 g, 0.03 mols) and p-toluene sulfonic acid (0.04 g, 0.2 mmols) in toluene (25 ml) was heated at 125° C. for 2 h; 15 ml of solvent was then distilled. To the residual orange solution was added sodium ethoxide (2 M, 15 ml) in ethanol (reaction mixture turns red). This red mixture was stirred at 120° C. for 2 h; 15 ml of solvent was again distilled. The reaction mixture was left to cool to room temperature, diluted with ethyl acetate (1 litre), extracted with HCl 0.1 M and water. The combined organic extracts were dried over sodium sulfate and concentrated in vacuo to leave an orange residue which was washed once with cold ethyl acetate to yield 2-cyclo-propyl-4-oxo-1,4-dihydro-quinoline-3-carboxylic acid ethyl ester (3.87 g, 53%) as an off-white solid. LCMS m/z 244.14 [M+H]+ @ RT 0.78 min, 89%, m/z 230.11 [Acid+H]+ @ RT 1.27, 11%.

δH(400 MHz, (CD3)2SO) 11.04 (1 H, s), 8.06 (1 H, dd, J1 1.1, J2 8.1), 7.76-7.66 (2 H, m), 7.36 (1 H, td, J1 1.1, J2 7.5), 3.89 (3 H, s), 2.16 (1 H, m), 1.18 (4 H, d, J 7.0).

Step 2

4-Chloro-2-cyclopropyl-quinoline-3-carboxylic acid ethyl ester

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Phosphorus oxychloride (0.77 ml, 0.082 mols) was added in one portion to a suspension of 2-cyclopropyl-4-oxo-1,4-dihydro-quinoline-3-carboxylic acid ethyl ester (1.0 g, 0.041 mols) in acetonitrile and the mixture was heated at 75° C. for 90 minutes (becomes a clear solution above 65° C.). The resulting light brown solution was poured into saturated sodium bicarbonate (100 ml); the suspension was extracted with ethyl acetate and the combined organic extracts were dried and concentrated in vacuo to leave 4-Chloro-2-cyclopropyl-quinoline-3-carboxylic acid ethyl ester (1.15 g, 106%) as an off-white solid. Rf(AcOEt)=0.73.

Step 3

4-(4-cyclopropyl-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-benzoic acid

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4-Chloro-2-cyclopropyl-quinoline-3-carboxylic acid ethyl ester (1.15 g, 0.0041 mols) and 4-hydrazino-benzoic acid (1.0 g, 0.0068 mols) were stirred in ethanol (30 ml) at reflux for 16 h. The bright yellow suspension was diluted with heptane, filtered, washed with cold t-butylmethyl ether and left to dry under suction to yield crude solid containing hydrazine. This solid was suspended in 1 M HCl, filtered, washed with water and then dried in vacuo to yield 4-(4-cyclopropyl-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-benzoic acid (1.135 g, 80%) as a yellow solid, LCMS m/z 346.20 [M+H]+ @ RT 1.05 min: 96% purity.

δH(400 MHz, (CD3)2SO) 11.4 (1 H, s), 8.43 (2 H, d, J 8.1), 8.21 (1 H, dd, J1 1.2, J2 8.1), 8.07 (2 H, d, J 8.1), 7.92 (1 H, d, J 8.1), 7.67 (1 H, t, J 6.6), 7.52 (1 H, t, J 6.5), 3.43 (1 H, m), 1.59 (2 H, m), 1.43 (2 H, m).

Step 4

4-(4-cyclopropyl-3-oxo-3,5-dihydro-pyrazolo[4,3-c]-quinolin-2-yl)-benzoyl chloride

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To a suspension of finely ground 4-(4-cyclopropyl-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-benzoic acid (0.19 g. 0.55 mmol) in dichloromethane (4 ml) was added oxalyl chloride (1.6 ml, 0.01 mol) followed by a drop of dimethyl formamide. The mixture was stirred under nitrogen at 45° C. for 8 h. The solvent was removed in vacuo to yield 4-(4-cyclopropyl-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-benzoyl chloride as a pale yellow solid, LCMS m/z [M+MeOH—Cl]+ @ RT 1.46 min: 95% purity. Used without further purification.

Step 5

N-(3-Dimethylamino propyl)-4-(4-cyclopropyl-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl]-benzamide

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To a partial solution of 4-(4-cyclopropyl-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-benzoyl chloride (0.1 g, 0.28 mmol) in tetrahydrofurane (6 ml) under nitrogen was added a solution of 3-dimethylamino-propyl amine (0.03 g, 0.3 mmol) in tetrahydrofurane (3 ml). The mixture was stirred at RT for 3 h. The solvent was removed under reduced pressure and the yellow solid was washed with a little saturated sodium bicarbonate, water and dried under vacuo to yield N-(3-Dimethylamino propyl)-4-(4-cyclopropyl-3-oxo-3,5-dihydro-pyrazolo[4,3-c]-quinolin-2-yl]-benzamide (57 mg, 47%) as a yellow solid. LCMS m/z 430.11 [M+H]+ @ RT 0.99 min: 100% purity.

    • Activity ***

Preparation of Intermediate 5

4-(6-Fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl]-benzoyl chloride

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To a suspension of finely ground 4-(6-Fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl]-benzoic acid (1.1 g. 3.4 mmol) in dichloromethane (6 ml) was added oxalyl chloride (2.4 ml, 29 mmol) followed by a drop of dimethyl formamide. The mixture was stirred under nitrogen at 45° C. for 3 h. The solvent was removed in vacuum to yield 4-(6-Fluoro-3-oxo-3,5-dihydro-pyrazolo[4, 3-c]quinolin-2-yl]-benzoyl chloride (1.15 g, quantitative) as a pale yellow solid that was used without further purification.

EXAMPLE 66

N-(3-Dimethylamino propyl)-4-(6-fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl]-benzamide hydrochloride

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To a partial solution of 4-(6-Fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl]-benzoyl chloride (0.1 g, 0.3 mmol) in tetrahydrofurane (5 ml) under nitrogen was added a solution of 3-dimethylamino-propyl amine (0.03 g, 0.3 mmol) in tetrahydrofurane. The mixture was stirred at rt for 90 minutes. The solvent was removed under reduced pressure and the yellow solid was purified via FCC silica gel (gradient elution, MeOH:H2O, Fluka C18 reverse phase) to yield N-(3-Dimethylamino propyl)-4-(6-fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl]-benzamide hydrochloride (70 mg, 53%) as a yellow solid.

LCMS m/z 408.39 [M+H]+ @ RT 0.89 min: 90% purity.

    • Activity ***

EXMAPLES 67-141

Were Prepared Analogously From the Appropriate Benzoyl Chloride and the Appropriate Amine

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M.S.
ExampleXZWRR′(MH+)Activity
676-FHH—CH2CH2CH2CH2CH2391.3**
686-FHH—CH2PhenylH413.2***
696-FHH—CH2PhenylMe427.3**
706-FHH—CH2CH2OMeH381.2***
716-FHH—CH2CH2N(Me)2H394.3***
726-FHH—CH2CO2MeH395.3***
736-FHH—CH2CH2CH2OMeH395.2***
746-FHH—CH2CH2CH2N(Me)2H408.3***
756-FHH embedded image H431.3**
766-FHH embedded image H419.2**
776-FHHEtH351.2***
786-FHHEtEt379.3**
796-FHH embedded image H420.4***
806-FHH—CH2CH2CH2N(Me)2Me422.4***
816-FHH—CH2CH2CH2CH2N(Me)2H422.4***
826-FHH embedded image H448.5***
836-FHH embedded image H434.4***
846-FHH embedded image H525.3***
856-FHH—CH2CH2CH2CH2CH2N(Me)2H450.3***
86HHH—CH2CH2CH2N(Me)2H390.2***
87HHH—CH2CH2CH2CH2CH2N(Me)2H432.1**
88HHH—CH2CH2CH2CH2N(Et)2H432.2**
89HHH—CH2CH2CH2N(Me)2Me404.2**
906-FH2—Cl—CH2CH2CH2N(Me)2H442.1**
91HHH embedded image H416.1**
92HHH embedded image H573.0**
93HHH embedded image H445.1**
94HHH embedded image H507.1**
956-FHH embedded image H591.0***
96H embedded image H—CH2CH2CH2N(Me)2H430.1***
976-FHH embedded image H464.1***
986-FHH embedded image H463.1***
996-FH3—Cl embedded image H482.1**
1006-FH2—Cl embedded image H497.1**
1026-FH2—Cl—CH2CH2CH2CH2N(Et)2H484.1**
1036-FH3—Cl—CH2CH2CH2N(Me)2H442.1**
104H embedded image H embedded image H470.4***
1056-FHH embedded image 516.3*
1066-FHH embedded image H470.3***
1076-FHH—CH2CH2N(iPr)2H451.4***
1086-FH2—Cl embedded image H496.2**
1096-FHH embedded image H456.1***
1106-FH2—Cl—CH2CH2CH2CH2N(Me)2H456.1**
1116-FHH embedded image 406.2**
1126-FHH embedded image H462.1***
1136-FHH embedded image H436.1***
1146-FHH embedded image H434.4***
1156-FHH embedded image H476.1***
1166-FHH embedded image H496.1***
1176-FHH embedded image H436.3***
1186-FHH embedded image H462.3***
1196-FHH embedded image H428.1**
1206-FHH—CH2CH2SEtH411.3***
1216-FHH embedded image H448.3**
1226-FHH embedded image H431.3***
1236-FHH embedded image H434.3**
1246-FHH—CH2CH2CH2CH2N(Et)2H450.4***
1256-F embedded image H embedded image H536.1***
1266-F embedded image H embedded image H516.2***
1276-FHH embedded image H428.3*
1286-FHH—CH2CH2CH2SMeH411.3**
129H embedded image H embedded image H498.5***
1306-F embedded image H embedded image H488.4***
1316-FHH embedded image H446.3***
1326-F embedded image H—CH2CH2CH2N(Me)2H448.2***
1336-F embedded image H embedded image H502.3***
1346-F embedded image H embedded image H486.3***
1356-F embedded image H—CH2CH2CH2CH2N(Et)2H490.3***
1366-F embedded image H embedded image H546.2**
1376-F embedded image H embedded image H631.2***
1386-F embedded image H embedded image H468.2**
1396-F embedded image H embedded image H468.2*
1406-F embedded image H embedded image H476.2***
1416-F embedded image H embedded image H474.3***

EXAMPLE 142

{3-[4-(6-Fluoro-3-oxo-3,5-dihydro-pyrazolo[4,3-c]quinolin-2-yl)-phenyl]-ureido}acetic acid ethyl ester

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Ethyl cyanatoacetate (31 mg, 0.24 mmol) was added in one portion to a stirred solution of 2-(4-aminophenyl)-6-fluoro-2,5-dihydropyrazolo[4,3-c]quinolin-3-one (intermediate 2) (50 mg, 0.17 mmol) in N,N-dimethylformamide (2 ml) and the mixture stirred at room temperature for 16 h. Water (1 ml) was then added to the mixture to precipitate a solid, which was filtered, washed with water (1 ml) and then ethyl acetate (1 ml) and finally dried by suction to leave the urea as a yellow solid, LCMS m/z 424.40 [M+H]+ @ RT 1.06 min.

    • Activity ***

EXAMPLES 143 and 144

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Example 143
LCMS m/z 438.41 [M + H]+@ RT 1.13 min.
Activity **
embedded image
Example 144
LCMS m/z 514.46 [M + H]+@ RT 1.35 min.
Activity *

The following compounds were synthesised by the method of Example 142, substituting the appropriate isocyanate, isothiocyanate or chloroformate for ethyl cyanatoacetate.

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M.S.
ExampleXZYRA(MH +)Activity
1446-FHOiPrNH380.3***
1456-FHOnPrNH380.3***
1466-FHOtBuNH394.4***
1476-FHOPhNH414.3**
1486-FHS embedded image NH394.3**
1496-FHS embedded image NH436.4*
1506-FHOtBuO395.3***
1516-FHOEtO367.2**
1526-FHOCH2CH2N(Me)2O410.2***
153H embedded image OMeO375.3**
1546-FHOCH2CH2CH2N(Me)2O424.1***
1556-FHO embedded image O512.3**
1566-FHSnPentylNH424.4**
1576-FHSCH(CH3)CH(CH3)CH3NH424.4**
1586-FHOCH2CH2CH2CH2N(Et)2NH465.4***
159HHOnPrNH362.3***
160HHS embedded image NH376.1**
1616-FHOCH2CH2CH2N(Me)2NH423.3***
162HHO embedded image NH434.5***
1636-FHOCH2CH2CH2CH2N(Me)2NH437.2***
1646-F HO embedded image NH463.5***

Intermediate 6: Preparation of methyl 4-oxothiochromane-3-carboxylate

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Dry tetrahydrofuran (60 ml) was cooled under nitrogen atmosphere to −50 to −60° C. 1M Lithium bis(trimethylsily)amide solution in hexane (56 ml, 56 mmol) was added. The temperature was kept at −50 to −60° C. and thiochroman-4-one was added dropwise over 20 min. Stirring was continued at low temperature for 60 min. Methyl cyanoformate (4.84 ml, 60.9 mmol) was added dropwise over 5 min to the reaction mixture. The obtained suspension was stirred at −50 to −60° C. for 80 min and then allowed to warm up to room temperature. Saturated ammonium chloride solution (100 ml) was added. The phases were separated, the aqueous phase extracted with ethyl acetate (2×100 ml). The combined organic phases were washed with water (50 ml), dried over magnesium sulphate, filtered and concentrated under vacuum. An orange oil was obtained and purified by column chromatography. The title compound was isolated as a yellow solid (4.70 g, 21.1 mmol, 42%). LCMS: m/z 221 [M−H]+.

Intermediate 7: Preparation of 4-(3-Oxo-3a,4-dihydro-3H-thiochromeno[4,3-c]pyrazol-2-yl)-benzoic acid

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4-Oxothiochromane-3-carboxylate (0.50 g, 2.25 mmol) and hydrazinobenzoic acid (0.377 g, 2.48 mmol) were mixed in acetic acid (6 ml). The mixture was heated to reflux for 30 min. Excess acetic acid was distilled off to give a brown oil. Diethylether was added, a precipitate formed which was collected by filtration and dried under vacuum. The crude product was isolated as a red/brown solid (797 mg). LCMS: m/z 325 [M+H]+. No purification was carried out.

Intermediate 8: Preparation of 4-(3-oxothiochromeno[4,3-c]pyrazol-2(3H)-yl)benzoic acid

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Crude 4-(3-Oxo-3a,4-dihydro-3H-thiochromeno[4,3-c]pyrazol-2-yl)-benzoic acid (250 mg, 0.77 mmol) was dissolved in dimethyl sulphoxide (6 ml). O-Chloranil (189 mg, 0.77 mmol) was added and the mixture was stirred at room temperature overnight. Water (20 ml) was added and the solids were collected by filtration and washed with water. The filter cake was triturated with toluene, filtered and dried under vacuum. The title compound was isolated as a dark brown solid (230 mg, 0.71 mmol, 92%). LCMS: m/z 323 [M+H]+

Alternatively crude 4-(3-Oxo-3a,4-dihydro-3H-thiochromeno[4,3-c]pyrazol-2-yl)-benzoic acid can be stirred in dimethyl sulphoxide under exposure to air. It was found that air oxidation provides clean product, however the reaction is much slower.

EXAMPLE 165

Preparation of N-[3-(dimethylamino)propyl]-4-(3-oxothiochromeno[4,3-c]pyrazol-2(3H)-yl)benzamide

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4-(3-oxothiochromeno[4,3-c]pyrazol-2(3H)-yl)benzoic acid (55 mg, 0.17 mmol) was suspended in anhydrous dimethyl acetamide (1 ml). Diisopropyl-ethyl amine (46.5 mg, 0.36 mmol, 62μl) was added followed by 3-dimethylaminopropylamine (17.5 mg, 0.17 mmol) and [(benzotriazol-1-yloxy)-dimethylamino-methylene]-dimethyl-ammonium hexafluoro phosphate (65 mg, 0.17 mmol). The mixture was stirred at room temperature for 4 h and was purified by preparative HPLC. The title compound was isolated as a brown solid. LCMS: m/z 407 [M+H]+

    • Activity **

EXAMPLE 166

Preparation of N-[(cyclohexylamino)propyl]-4-(3-oxothiochromeno[4,3-c]pyrazol-2(3H)-yl)benzamide

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The reaction was carried out as described above. LCMS: m/z 461 [M+H]+

    • Activity ***

EXAMPLE 167

Preparation of N-(pyrrolidin-1-yl-butyl)-4-(3-oxothiochromeno[4,3-c]pyrazol-2(3H)-yl)benzamide

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The reaction was carried out as described above. LCMS: m/z 447 [M+H]+

    • Activity *

EXAMPLE 168

Preparation of 4-(3-oxothiochromeno[4,3-c]pyrazol-2(3H)-yl)-N-1,2,2,6,6-pentamethylpiperidin-4-ylbenzamide

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The reaction was carried out as described above. LCMS: m/z 475 [M+H]+

    • Activity **

Intermediate 9: Preparation of 3-[(2-fluorophenyl)sulfanyl]propanoic acid

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2-Fluorothiophenol (5.0 g, 39 mmol) was dissolved in tetrahydrofuran (50 ml) under a nitrogen atmosphere. Triethylamine (3.94 g, 5.33 ml, 85.8 mmol) was added. Acrylic acid (2.81 g, 2.67 ml, 39 mmol) was dissolved in tetrahydrofuran and added dropwise to the reaction solution over 2 h at room temperature. The mixture was stirred at room temperature overnight. 1M Hydrochloric acid (50 ml) was added and the phases were separated. The aqueous phase was washed with ethyl acetate (2×50 ml). The combined organic phases were dried over magnesium sulphate, filtered and concentrated under vacuum. A yellow oil was obtained which solidified upon storage at room temperature. The solid was triturated with hexane, filtered and dried under vacuum. The title compound was isolated as an off-white solid (4.19 g, 20.9 mmol, 54%).

Intermediate 10: Preparation of 8-fluoro-2,3-dihydro-4H-thiochromen-4-one

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3-[(2-Fluorophenyl)sulfanyl]propanoic acid (4.0 g, 20 mmol) was mixed with concentrated sulphuric acid (20 ml) at 0-5° C. The reaction solution was stirred at 0 to 5° C. for 3 h then allowed to warm up to room temperature overnight. The mixture was quenched dropwise into ice to give a white suspension. The aqueous phase was extracted with ethyl acetate (1×200 ml, 1×100 ml). The combined organic phases were washed with saturated sodium bicarbonate solution (1×50 ml), water (1×50 ml), 1M hydrochloric acid (50 ml) and water (2×50 ml). The organic phase was dried over magnesium sulphate, filtered and concentrated under vacuum. The title compound was isolated as a yellow solid (2.10 g, 11.5 mmol, 58%).

Intermediate 11: Preparation of methyl 8-fluoro-4-oxothiochromane-3-carboxylate

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1M Lithium hexamethyldisilazide solution in hexane (13.2 ml) was dissolved in anhydrous tetrahydrofuran (20 ml) under nitrogen atmosphere. The solution was cooled to −78° C. 8-Fluoro-2,3-dihydro-4H-thiochromen-4-one (2.00 g, 11 mmol) was dissolved in tetrahydrofuran (40 ml), the solution was transferred to the dropping funnel and added dropwise over 30 min to the reaction mixture maintaining the temperature below −60° C. An orange clear solution was obtained which was stirred at −78° C. to −65° C. for 2 h. Methyl cyanoformate (0.935 g, 0.87 ml) was dissolved in tetrahydrofuran (2 ml) and added dropwise to the reaction solution. Stirring was continued at low temperature for 1 h, the mixture was then allowed to warm to room temperature. Saturated ammonium chloride solution (20 ml) and water (10 ml) were added, the phases mixed for 5 min and separated. The aqueous phase was washed with ethyl acetate (2×100 ml) and the combined organic phases were dried over magnesium sulphate. The mixture was filtered and the solvent removed under vacuum to give an orange oil. The crude oil was purified by column chromatography; mobile phase: hexanes, gradient to hexanes/ethyl acetate [90:10]. The title compound was isolated as a yellow solid (1.19 g, 4.95 mmol, 45%).

Intermediate 12: Preparation of 4-(6-fluoro-3-oxothiochromeno[4,3-c]pyrazol-2(3H)-yl)benzoic acid

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Methyl 8-fluoro-4-oxothiochromane-3-carboxylate (1.19 g, 4.95 mmol) and 4-hydrazinobenzoic acid (755 mg, 4.95 mmol) were mixed with glacial acetic acid (10 ml). The mixture was heated to reflux for 4 h. Excess acetic acid was removed under vacuum to give an orange oil. Ethyl acetate (10 ml) was added and the mixture sonicated. Precipitation of an orange solid was observed. The solids were collected by filtration and washed with ethyl acetate. The filter cake was taken up in dimethyl suphoxide (10 ml) and air-oxidised at room temperature for one week. Water (20 ml) was added to the reaction mixture, the solids were collected by filtration, slurried in ethyl acetate, filtered and dried under vacuum. The title compound was isolated as an orange powder (175 mg, 0.51 mmol, 10%). LCMS: m/z 341.

EXAMPLE 169

Preparation of N-[3-(dimethylamino)propyl]-4-(6-fluoro-3-oxothiochromeno[4,3-c]pyrazol-2(3H)-yl)benzamide

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4-(6-Fluoro-3-oxothiochromeno[4,3-c]pyrazol-2(3H)-yl)benzoic acid (41 mg, 0.12 mmol) was dissolved in anhydrous dimethyl-acetamide(1 ml). Diisopropyl-ethyl amine (46 mg, 0.36 mmol, 62 μl) was added followed by [(benzotriazol-1-yloxy)-dimethylamino-methylene]-dimethyl-ammonium hexafluoro phosphate (65 mg, 0.17 mmol) and 3-dimethylaminopropylamine (12 mg, 0.12 mmol). The mixture was stirred at room temperature overnight and purified by preparative HPLC. The title compound was isolated as a brown solid. LCMS: m/z 425 [M+H]+.

    • Activity **

EXAMPLE 170

Preparation of N-[(cyclohexylamino)propyl]-4-(6-fluoro-3-oxothiochromeno[4,3-c]pyrazol-2(3H)-yl)benzamide

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The reaction was carried out as described above. LCMS: m/z 479 [M+H]+.

    • Activity **

EXAMPLE 171

Preparation of N-(pyrrolidin-1-yl-butyl)-4-(6-fluoro-3-oxothiochromeno[4,3-c]pyrazol-2(3H)-yl)benzamide

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The reaction was carried out as described above. LCMS: m/z 465 [M+H]+.

    • Activity ***

EXAMPLE 173

Preparation of 4-(6-fluoro-3-oxothiochromeno[4,3-c]pyrazol-2(3H)-yl)-N-1,2,2,6,6-pentamethylpiperidin-4-ylbenzamide

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The reaction was carried out as described above. LCMS: m/z 493 [M+H]+

    • Activity ***

Assay Section

The examples described above were tested in a cell free Homogenous Time Resolved Fluorescence (HTRF) assay to determine their activity as inhibitors of the CD80-CD28 interaction.

In the assay, europium and allophycocyanin (APC) are associated with CD28 and CD80 indirectly (through antibody linkers) to form a complex, which brings the europium and APC into close proximity to generate a signal. The complex comprises the following six proteins: fluorescent label 1, linker antibody 1, CD28 fusion protein, CD80 fusion protein, linker antibody 2, and fluorescent label 2. The table below describes these reagents in greater detail.

FluorescentAnti-Rabbit IgG labelled with Europium
label 1(1 μg/ml)
LinkerRabbit IgG specific for mouse Fc
antibody 1fragment (3 μg/ml)
CD28 fusionCD28 - mouse Fc fragment fusion protein
protein(0.48 μg/ml)
CD80 fusionCD80 mouse Fab fragment (C215) fusion
proteinprotein (1.9 μg/ml)
LinkerGαMκ-biotin: biotinylated goat IgG
antibody 2specific for mouse kappa chain (2 μg/ml)
FluorescentSA-APC: streptavidin labelled
label 2allophycocyanin (8 μg/ml)

On formation of the complex, europium and APC are brought into proximity and a signal is generated.

Non-specific interaction was measured by substituting a mouse Fab fragment (C215) for the CD80 mouse Fab fragment fusion protein (1.9 μg/ml). The assay was carried out in black 384 well plates in a final volume of 30μl. Assay buffer: 50 mM Tris-HCl, 150 mM NaCl pH 7.8, containing 0.1% BSA (w/v) added just prior to use.

Compounds were added to the above reagents in a concentration series ranging between 100 μM-1.7 nM. The reaction was incubated for 4 hours at room temperature. Dual measurements were made using a Wallac Victor 1420 Multilabel Counter. First measurement: excitation 340 nm, emission 665 nm, delay 50 μs, window time 200 μs. second measurement: excitation 340 nm, emission 615 nm, delay 50 μs, window time 200 μs. Counts were automatically corrected for fluorescence crossover, quenching and background.

By way of illustration, the EC50 results for the compounds of Examples 15, 21, 29, 35 and 83 were 8 μM, 1.9 μM, 950 nM, 148 nM and 90 nM respectively. For convenience, the EC50 activities of compounds tested are recorded above in summary form as:
EC50: *=>10 μM, **=1-10 μM, ***=<1 μM.