Matches 1 - 50 out of 77 1 2 >


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DE102019204850A1 Verfahren zur Vervielfältigung von DNA, Rotationsvorrichtung und System zur Vervielfältigung von DNA  
Bei einem erfindungsgemäßen Verfahren zur Vervielfältigung von DNA, wird verfahrensgemäß- ein Probenträger (4) mit mindestens einer Kavität (20), in der eine Probenflüssigkeit, die DNA enthält,...
DE102018125376B4 A broad spectrum of recombinant photosynthetic protein molecule and its method of construction  
A broad absorption spectrum recombinant photosynthetic protein molecule, characterized in that the recombinant photosynthetic protein molecule is obtained by the in vitro combination of...
DE102019106699A1 Device and method for the thermal treatment of samples  
The invention relates to a device for the thermal treatment of samples contained in sample vessels (9), comprising: a sample block (4) with a receiving area for the sample vessels (9); a first...
DE102019001425A1 CoLi - cyclic exponential de novo synthesis of double-stranded polynucleotides  
The methods for the chemical synthesis of single-stranded ss-nucleic acids, ssDNA and ssRNA-OLIGO-nucleotides (OLIGOS) have made a decisive contribution to providing double-stranded DNA and RNA...
DE202020003110U1 Conductivity thermal cycler  
A thermal cycler for carrying out a polymerase chain reaction, another polymerase reaction or a ligase reaction, characterized in that electrodes are fitted in the reaction chambers of this...
DE102019114011B3 Method and device for extracting and / or reproducing a target nucleic acid  
The invention relates to a method for extracting a target nucleic acid (22) from a sample liquid (500). The method comprises providing a heating device with a heating element (10) in contact with...
DE102018116353B4 Method for the detection and enumeration of epigenetic immune cells in human blood samples for immunodiagnostics and newborn screening  
A method for an improved methylation assay for identifying blood immune cells, comprising the steps of a) providing a sample of human blood, in particular from a newborn, comprising genomic DNA...
DE102018112644B4 CXCR3 as an epigenetic marker for the identification of inflammatory immune cells, in particular CD8 + memory T cells  
A method for specifically identifying the CXC motif chemokine receptor 3 (CXCR3) subpopulation of immune cells in a mammalian sample comprising immune cells, comprising analyzing the methylation...
DE102019108155B3 Micro drop retention assembly  
The invention relates to a microdrop retention arrangement, a method for operating and the use of this arrangement according to the preamble of the claims, in particular for microfluidic systems....
DE102018219483A1 Method and device for analyzing biological material  
The invention relates to a method for the analysis of biological material. The method has a step of reading in a measurement signal (120), a first reference signal (131) and a second reference...
DE102018213027A1 Reaction mixture, method and kit for carrying out a quantitative real-time PCR  
In the case of a reaction mixture for providing a reaction batch for carrying out a quantitative real-time PCR, the reaction mixture contains at least one target DNA (11), which corresponds at...
DE102018213026A1 Procedure for performing real-time PCR  
In a method for carrying out a process for the amplification of nucleic acids (10), sample nucleic acids and reference nucleic acids are amplified in separate reaction batches. Signals of the...
DE102018125376A1 A recombinant photosynthetic protein molecule with a broad absorption spectrum and its construction method  
The invention discloses a recombinant photosynthetic protein molecule with a broad absorption spectrum, which is obtained by combining recombinant chlorophyll-binding protein, phycobiliprotein...
DE102018116353A1 Method for the detection and enumeration of epigenetic immune cells in human blood samples for immunodiagnostics and newborn screening  
The present invention relates to improved methods for the detection and enumeration of epigenetic blood and immune cells, and corresponding applications and kits.
DE102019005689A1 COMPOSITIONS AND METHOD FOR DETECTING THE NUCLEIC ACID OF GROUP-B-STREPTOCOCCUS  
Disclosed are nucleic acid oligomers, including amplification oligomers and detection probes, for detecting the nucleic acid of Group B Streptococcus (GBS; Streptococcus agalactiae). Methods for...
DE102018001586A1 Method for detecting the amplification of a nucleic acid with improved specificity  
The present invention relates to a method for amplifying a nucleic acid with improved specificity using particular primer oligonucleotides and controller nucleotides, wherein in a first...
DE102018001873B4 Molecular methods to assess complications after a kidney transplant  
A method of screening a human for expression of an RNA, which is associated with a complication after kidney transplantation, the method comprising comparing an expression of the RNA in a vesicle,...
DE102017124998B3 Method and device for determining the blood type of a cat in the AB blood group system  
The invention relates to a device and a method for determining the blood group of a cat in the AB blood group system, which comprises the following steps: a) providing a biological sample from the...
DE102016120124B4 A method of conducting a polymerase chain reaction and apparatus for carrying out the method  
A method of amplifying nucleic acids by means of a polymerase chain reaction in a reaction volume in which the reaction volume is heated by the use of electrical energy, characterized in that in...
DE112016003951T5 MOLECULAR METHODS FOR EVALUATING COMPLICATIONS AFTER A KIDNEY TRANSLATION  
The present invention relates to methods for obtaining exosomes and microvesicles (EMV) from urine, for isolating corresponding mRNA, and for studying expression patterns to diagnose and treat...
DE102016120124A1 Method for performing a polymerase chain reaction and apparatus for carrying out the method  
The invention relates to a method for multiplying nucleic acids by means of a polymerase chain reaction in a reaction volume, in which the reaction volume is heated by the use of electrical...
DE102016124844B3 DNA probes for in situ hybridization of chromosomes  
Kit, probe mixtures and probes for the detection of a chromosome aberration. A gene probe obtained by a method comprising the following steps: (a) examining genomic sections for sequence regions...
DE19941756B4 Method for using oligomeric nucleic acid-protein conjugates as reagents for immunological detection methods, in particular immuno-PCR  
Oligomeric protein-nucleic acid conjugates, formed by self-assembly consisting of a.) nucleic acids linked to at least two binding molecules, and b.) Proteins which have at least two binding sites...
DE10009143B4 Detection of human papilloma viruses  
Method for the detection of human papillomavirus (HPV) in biological material, with the steps: a) extraction / isolation of nucleic acids from the biological material, and b) detection of...
DE19824900B4 DNA evidence of a Strangreassoziationskomplex  
process containing for the selective detection of a nucleic acid, the steps - amplification the nucleic acid or a part thereof with the aid of two primers, one of which with one strand of the...
DE10150121B4 Real time detection of DNA amplification  
process to perform a Nukleinsäureamplifikaktion, in which transcribed in a single step reaction RNA reverse, without a Inhibiting reverse transcription occurs to the formed complementary DNA...
DE19755642B4 Labeled primer for the polymerase chain reaction  
Verwendung einer Polymerasekettenreaktion, bei der – mindestens einer der Primer an den beiden Enden seines Oliganukleotid-Stranges mit zwei unterschiedlichen Fluereszenzfarbstoffen, einem...
DE10065814B4 A method for simultaneous amplification of many sequences in a PCR reaction and its marking  
A method for the analysis of cytosine methylation in nucleic acids, characterized in that the following steps are performed: a) a nucleic acid sample is reacted with a reagent, whereby...
DE19825314B4 Methods for coupling SSCP and DGGE to improved detection of point mutations  
The claims cover the coupling of SSCP and DGGE on the application of an inserted primer with GC-adapter sequence with respect to the described in the following mutation screening strategy: SSCP...
DE10202892A1 Method for the simultaneous, quantifiable determination of infectologically relevant germs  
The invention relates to a method for the simultaneous, quantifiable determination of infectious germs, especially bacteria and viruses, by means of an analysis using a polymerase chain reaction...
DE10150121A1 Real-time detection of DNA amplification products  
The invention relates to a method for the real-time detection of DNA amplification products. The invention also relates to partially double-stranded oligonucleotides, which are used during the...
DE10115748A1 Control plasmid, useful in detection of Mycoplasma pneumoniae by polymerase chain reaction, includes amplicon between specific primer sequences  
Control plasmid (A) that includes (i) primers (P) that recognize a species-specific segment (S) of the P1 adhesin protein gene of Mycoplasma pneumoniae and (ii) between P, an amplicon containing...
DE10065814A1 Method for the simultaneous amplification of many sequences in a PCR reaction and their labeling  
A method for the amplification of nucleic acids is disclosed, whereby the section for amplification is first hybridised with at least two primer oligonucleotides, comprising two domains, of which...
DE10051714A1 Controlling performance of complex polymerase chain reaction amplification, useful e.g. for genotyping, using a set of many specific primers and non-specific counter-strand primers  
Controllable performance method of complex polymerase chain reaction (PCR) amplifications. First, PCR is carried out with at least 50 different primers (P1) of one type, complementary to one...
DE10038733A1 A method for determining the age of individuals  
Disclosed is a method for determining the age of individuals on the basis of sperm samples, characterized in that one determines the age of information through analysis of DNA methylation patterns...
DE10049364A1 Kit, methods and microarray for determining the sex of a human fetus  
The invention relates to a diagnosis kit for determining the sex of a human foetus from a maternal blood sample, comprising at least one pair of oligonucleotides (reverse primer, forward primer),...
DE19958980A1 Method for determination of PCR products, and a PCR detection kit and a device for such a method  
For the determination of PCR products, wherein amplified by at least one target sequence PCR products are measured in a flow cytometer and two different labels are incorporated into the...
DE10009143A1 Detection of human papillomavirus  
The invention relates to a method for detecting human papilloma viruses (HPV) in biological material. According to said method, nucleic acids are extracted/isolated from biological material and...
DE19956203A1 Controlling performance of complex polymerase chain reaction amplification, useful e.g. for genotyping, using a set of many specific primers and non-specific counter-strand primers  
Controllable performance method of complex polymerase chain reaction (PCR) amplifications. First, PCR is carried out with at least 50 different primers (P1) of one type, complementary to one...
DE19941756A1 Use of oligomeric conjugates of nucleic acids and proteins as immunoassay reagents, especially as labels that can be amplified by polymerase chain reaction  
Using oligomeric conjugates (I) of nucleic acids and proteins, as immunoassay reagents, is new. Independent claims are also included for the following: (1) a competitive immunoassay in which the...
DE19935767A1 Selection of primer pairs for reverse transcription amplification, based on low homology between mRNA and pseudogene sequences, provide specific amplification of cDNA  
Selecting primer pairs for reverse transcriptase-polymerase chain reaction for specific cDNA amplification in mRNA expression analysis, taking into consideration the existence of pseudogenes (I),...
DE19906169A1 Test kit and method for quantitative detection of genetically modified DNA in food by fluorescence-coupled PCR  
The invention relates to a test kit and a specific method for qualitatively and quantitatively detecting DNA in foodstuff. The invention also relates to foodstuff products which come from...
DE19854946A1 Cloning and copying at surfaces  
The invention relates to a method for cloning and copying genetic material on surfaces as well as copying biological material insofar as it, in a broader sense, can be classified in a ligand...
DE19825314A1 Mutation screening method comprises coupling single strand conformation polymorphism (SSCP) and denaturing gradient gel electrophoresis (DGGE)  
A mutation screening method (I), is new and comprises the coupling of single strand conformation polymorphism (SSCP) and denaturing gradient gel electrophoresis (DGGE) through the use of a nested...
DE19835856A1 Oligonucleotide primers and probes, for the detection of hepatitis B virus, are used to amplify, by polymerase chain reaction, a section of the hepatitis B virus genome  
A method of detecting hepatitis B virus (HBV) in a sample comprising exposing the sample to a polymerase chain reaction (PCR), where the PCR amplified DNA fragment corresponds to part of the HBV...
DE19834948A1 Detection of RNA using a reverse transcriptase primer comprising a first sequence which specifically hybridizes with the RNA, used to detect DNA contamination of samples  
A method for the specific detection of RNA in the presence of sequence identical DNA, where a reverse-transcriptase (RT) reaction is performed, by producing an RT primer with a first and second...
DE19824900A1 DNA evidence of a Strangreassoziationskomplex  
By combining labeled primers for the production of amplificates of one strand of a nucleic acid with a capture probe complementary to the same strand of the nucleic acid, increasing the...
DE19815668A1 Improved polymerase chain reaction method used to reduce the number of false results  
A polymerase chain reaction (PCR) method which includes the step of thoroughly heating the reaction vessel, before adding the sample, at a temperature and for a time sufficient for thermolysis of...
DE19814828A1 Nucleic acid amplification assay for detecting viral, bacterial, cellular, yeast or fungal nucleic acids  
Assay for a nucleic acid comprises: (a) generating amplification products from a fragment of the nucleic acid (b) contacting the amplification products with a probe; and (c) detecting...
DE19806850A1 Detecting target nucleic acids, especially single-stranded DNAs, using a labeled primer system  
A method for detecting single-stranded DNA in a sample is new and comprises contacting the sample with a forward and/or reverse primer (I), at least one of which carries at least part of a label...

Matches 1 - 50 out of 77 1 2 >