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DE102013008875B4 Process for enzymatic purine degradation  
Process for the enzymatic degradation of purines of all kinds in liquids and solids, characterized in that the purine-degrading enzyme mixture contains the enzymes purine nucleotide phosphorylase,...
DE102006035020B4 Stabilization of NAD / NADH  
A test element for the determination of an analyte comprising (i) a coenzyme dependent enzyme or a substrate for such an enzyme and (ii) as a coenzyme a compound having the following general...
DE102015209513A1 Measuring electrode for measuring a concentration of a substance in a tissue fluid, measuring device and method for producing a measuring electrode for measuring a concentration of a substance in a tissue fluid  
The invention relates to a measuring electrode (100) for measuring a concentration of a substance in a tissue fluid. The measuring electrode (100) has a carrier layer (102) on which at least one...
DE102014223539A1 Method, kit and biosensor for the determination of lactate  
In a method for the determination of lactate, the lactate is replaced by a lactate dehydrogenase in the presence of NAD+ to pyruvate and NADH + H+ implemented. The NADH formed is measured using a...
DE102013216886A1 Sensor, method and test kit for measuring glucose  
In a sensor (100; 200; 300; 400) for the detection of glucose is in or on a support (101; 301; 201) containing the sensor, the enzyme hexokinase. Furthermore, the sensor comprising the enzyme...
DE102013008875A1 A method for enzymatic Purinabbau  
The object of the invention is the enzymatic degradation of purines in liquids and solids of any kind.
DE102008061866B9 Use of ionic liquids  
Use of ionic liquids to increase the half-life of the cofactors NADPH and NADH.
DE102008061866B4 Use of ionic liquids  
Use of ionic liquids to increase the half-life of the cofactors NADPH and NADH.
DE102008061866A1 Use of ionic liquid comprising e.g. pyridinium cation for increasing the half-life of cofactors e.g. NADPH and NADH  
Use of ionic liquid to increase the half-life of cofactors, is claimed. An independent claim is also included for a sensor comprising a substrate, an immobilized enzyme and a cofactor, that is...
DE102008030435A1 Novel variants PQQ-dependent Glukosehydrogenase with improved substrate specificity  
The invention relates to novel PQQ-dependent soluble glucose dehydrogenase (sPQQGDH), which have increased substrate specificity compared to the wild type, and methods for their preparation and...
DE102006055604A1 A method for the diagnosis of diabetes mellitus  
The This invention relates to a method for the diagnosis of Diabetes mellitus by the detection of at least two polyols, and optionally additionally one or more mono-, di- and oligosaccharides and...
DE102006023897A1 Method and means for the enzymatic determination of ethanol  
The Invention relates to a method and means for the enzymatic Determination of ethanol. The inventive method prevents the disturbance of Detection by ethanol vapors in the ambient air.
DE29924971U1 A system for determining an analyte  
On Article which is suitable for use in determining the Presence or amount of an analyte in a biological sample by optical reading, the article comprising a multiple-layer element, comprising: (A)...
DE102006002165A1 Determining acetate content in a sample, comprises contacting a sample with test strip comprising e.g. tetrazolium salt, incubating the test strip, contacting test strip with phenazine methosulfate reagent and observing color change  
Method for determining acetate content in a sample, comprises: (a) providing a sample; (b) diluting and adjusting the pH of the diluted sample with a buffer comprising malic acid; (c) contacting...
DE102006035020A1 Test element for determining analytes, comprises stabilized coenzyme and coenzyme-dependent enzyme, used especially for determination of glucose  
A test element (A) for determination of an analyte comprises: (i) a coenzyme-dependent enzyme (E) or a substrate for E; and (ii) as a coenzyme, a stabilized NAD derivative (I), or its salts or...
DE102005011421A1 Determination of short-chain SRL alcohol (DHRS4) as a biomarker for inflammation and infection  
use short-chain SRL alcohol dehydrogenase (DHSR4; SEQ ID NO: 1) as humorealer Biomarkers for diagnostic ex vivo detection and for prognosis as well as the course and therapy of septic inflammation...
DE102004061849A1 New polynucleotide encoding insect acyl-CoA-dehydrogenase, useful as a target for identification of insecticides, also new protein, vector and recombinant cells  
Isolated polynucleotide (I) comprising a sequence that encodes a insect acyl-CoA-dehydrogenase polypeptide (II) comprising a defined 418 amino acid sequence (SEQ ID NO:2) given in the...
DE102004007983A1 Test element with single-layer reaction film  
The Invention relates to test elements and method for optical detection an analyte in a sample.
DE10108900B4 Ermittlung des patientenbezogenen Kariesrisikos  
Verfahren zur Bestimmung des patientenbezogenen Kariesrisikos unter Verwendung von Milchsäure und/oder Lactat in Proben aus dem Mundraum, wobei im Falle von Bioflüssigkeiten zu 0,1 ml...
DE10320259A1 Novel Glucose dehydrogenase and their preparation  
The Invention relates to novel PQQ-dependent soluble glucose dehydrogenase (sPQQGDH) from Acinetobacter as well as a process for their production by overexpression in suitable microbial expression...
DE10195824T5 A method for determining the ability of the reaction, the reaction of the lymphocytes in the blood to specific antigen  
A method for determining the ability of the reaction of the lymphocytes in the blood to a specific antigen, comprising the steps of: the T-lymphocyte is in the peripheral blood of the recipient...
DE10233676A1 New polypeptide allergens from fungi, useful in desensitizing vaccines and for diagnosis, also related nucleic acids, vectors and transformed cells  
Polypeptides allergens from fungi (I): (a) containing at least 11 consecutive amino acids (aa) from sequences (A1) or (A8), of 173 or 267 aa, respectively; or (b) comprising sequences (A4), (A5)...
DE10221846A1 Detecting an analyte by enzymatic reaction, useful specifically for measuring glucose in blood, based on reaction with coenzyme and inactive coenzyme-binding protein  
Detecting an analyte (I) by enzymatic reaction comprising treating a sample with a detection reagent (DR) that contains a coenzyme (Co) and a catalytically inactive (Co)-binding protein (BP). (Co)...
DE10221845A1 Detecting analyte by enzymatic reaction, useful specifically for measuring glucose in blood, based on reaction with enzyme-coenzyme complex  
Detecting an analyte (I) by enzymatic reaction comprising treating the sample with a detection reagent (DR) that comprises an enzyme-coenzyme complex (C), under conditions where regeneration of...
DE10221840A1 Production of polymer layers on a transparent support, for use in sensors, e.g. for blood analysis, comprises coating the support with a photopolymerizable liquid composition and irradiating the liquid through the support  
Production (M1) of polymer layers on a transparent support comprises coating the prepared support with a photopolymerizable liquid composition (I), irradiating (I) through the support so as to...
DE10217254A1 Prevention or treatment of tumors comprises treating eukaryotic cells with an agent that modulates the expression and/or function of proteins synthesized and/or secreted by tumors  
Prevention or treatment of tumors comprises treating eukaryotic cells with an agent (I) that modulates the expression and/or function of proteins synthesized and/or secreted by tumors and thereby...
DE10214082A1 New polypeptide allergens from fungi, useful in desensitizing vaccines and for diagnosis, also related nucleic acids, vectors and transformed cells  
Polypeptides allergens from fungi (I): (a) containing at least 11 consecutive amino acids (aa) from sequences (A1) or (A8), of 173 or 267 aa, respectively; or (b) comprising sequences (A4), (A5)...
DE10202893A1 Method for the specific determination of methylglyoxal in liquid and / or gaseous samples  
The invention relates to a method for specifically identifying methylglyoxal in liquid and/or gaseous samples. According to the invention, the methylglyoxal is first oxidatively converted to...
DE10160660A1 Polypeptides for the identification of fungicidally active compounds  
The invention relates to polypeptides from phytopathogenic fungi with the biological activity of an inosine monophosphate dehydrogenase, nucleic acids coding therefor, the use of the polypeptides...
DE10108900A1 Determining the patient-related risk of caries  
The invention relates to a method for determining the risk of caries in a patient by analysing the lactic acid and/or lactate in the saliva thereof. The invention also relates to a method for...
DE10032042A1 Electrochemical Einwegbiosensor for the quantitative determination of analyte concentrations in fluids  
The invention relates to a disposable bio-sensor, comprising a support material (1), upon which electrical conductors (2) and an electrode system, comprising a counter electrode (6) and a working...
DE10007531A1 New dihydroliponamide dehydrogenase from dogfish, useful as diaphorase for converting substrates to dye, in analysis, and for producing enantiomerically pure lipoic acid  
Protein (I) with the activity of a dihydroliponamide dehydrogenase is a homodimer of relative molecular weight 100-150 kDa (gel filtration) and relative molecular weight of the subunits 50 kDa...
DE10001529A1 The stabilized coenzyme solutions and their use for the determination of dehydrogenases or their substrates  
Stabilized aqueous solution of a coenzyme for hydrogen-transferring enzymes, characterized in that the solution contains NAD, NADP or a corresponding derivative in an oxidized or reduced form and...
DE19944226A1 A method for the detection of oxidized forms of soluble guanylate cyclase and method of screening for activators of soluble guanylate cyclase with oxidized heme iron  
The invention relates to a method for the detection of a soluble guanylate cyclase, whose haem iron is present in a trivalent oxidation state and a method for detecting chemical substances which...
DE19935967A1 New DNA encoding Arabidopsis thaliana 1-deoxy-D-xylulose-5-phosphate reductoisomerase for identifying modulators of the enzyme that can be used as herbicides, antibiotic or anti-malarial agents  
A DNA (I) which encodes Arabidopsis 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXPR) or its functional fragment, is new. Independent claims are also included for the following: (1) a DNA...
DE19826153A1 Method and apparatus for detecting a nucleotide sequence which may be present in a sample  
The invention relates to a method for preparing samples for detecting a nucleotide sequence by polymerase chain reaction (PCR), according to which a) an analysis solution is filled into at least...
DE19815685A1 Recombinant Rhodobacter sphaeroides 3-hydroxy fatty acid dehydrogenase gene and methods of production  
An isolated DNA molecule (I) comprising a structural gene that encodes a protein with the enzymatic activity of a 3-hydroxy fatty acid dehydrogenase (3-HBDH) from a microorganism, is new....
DE19814761A1 Method and means for the determination of proteinases  
The invention relates to a method and agent for detecting proteinases by means of an enzyme as proteinase substrate. Said method is characterised in that the enzyme is inactivated, incubated with...
DE19811313A1 New assay for inosine 5'-monophosphate dehydrogenase activity in blood, useful for monitoring immunosuppressive status of cancer or transplant patients  
An assay for determining the inosine 5'-monophosphate dehydrogenase (IMPDH) activity in mammalian blood is new and comprises performing an IMPDH-catalyzed reaction with a lysate of a blood cell...
DE19748490A1 Method and reagent for creatine kinase determination  
Procedure or Reagent for the photometric, enzymatic determination of creatine kinase (CK) in biological sample material by formation of ATP from creatine phosphate and ADP and detection of ATP...
DE19756238A1 Terminating agent for conjugation dehydrogenase reactions  
A quenching agent for terminating conjugation dehydrogenase (DH) reactions comprises a wetting agent (I). Specifically, the reaction is a glucose-6-phosphate DH (G6PDH) reaction and (I) is...
DE19627075A1 Modified lactate dehydrogenase, processes for their preparation and their use  
The invention concerns a modified lactate dehydrogenase which is covalently bonded to an activated polysaccharide, is simultaneously present in the intramolecularly cross-linked form and is stable...
DE19622090A1 A process for the removal of hemoglobin interference in the analysis of medical samples  
The present invention relates to a process for determining an analyte in a sample containing free haemoglobin by optical bichromatic measurement for a main and a secondary measuring wave length. A...
DE19619056A1 Determination of substrates of NAD+ and NADP+-dependent dehydrogenase  
Enzymatic-electrochemical method for the determination of substrates (A) of NAD<+>- and NADP<+>-dependent dehydrogenases comprises reacting an analyte with dehydrogenase and...
DE19545130A1 Methods and apparatus for a modular microsystems for high-precision chemical Quick analyzes and methods for making  
The invention relates to a method and a device for determining the concentration of an analyte, analyte mixture or biochemical analyte-equivalent substance (titrant) contained in a sample using at...
DE19543493A1 The stabilized coenzyme solutions and their use for the determination of dehydrogenases or their substrates in an alkaline medium  
The invention relates to stabilised coenzyme solutions which contain up to approximately 60mM of a heavy metal salt and optionally a complexing agent in a defined ratio with the heavy metal salt....
DE19628873A1 Cyclitol-ubiquinone oxido:reductase enzyme  
New enzyme capable of oxidising cyclitols and their derivs. has a molecular wt. of 87 kD and contains the amino acid sequence (A): FRVHPTIAPQNTTHPQE (A). Also claimed is DNA coding for the enzyme...
DE19520210A1 Determn. of tri:glyceride(s) in protein fractions  
Determn. of triglycerides in protein fractions comprises: (a) electrophoretic sepn. of the protein fractions in a suitable matrix, (b) enzymatic cleavage of the triglycerides and (c) determn. of...
DE3991428C2 Bile acid sulphate sulphatase produced by pseudomonas testosterone  
A new bile acid sulphate sulphatase hydrolyses the sulphate ester from the 3 beta-sulphate ester of bile acid, with inversion to the 3 alpha-hydroxy cpd. It is active on taro- and glycoholic acid...
DE4438206A1 Microbial cholesterol dehydrogenase, a process for their preparation and use  
Oxygen-independent cholesterol-spiking enzyme obtainable from Actinomycetes or Basidiomycetes using artificial electron acceptors, and a method and reagent for the determination of (total)...

Matches 1 - 50 out of 102 1 2 3 >