Title:
Retroviral protease inhibiting compounds
Kind Code:
A1


Abstract:
The present invention discloses novel compounds, compositions, and methods for inhibiting retroviral proteases and in particular for inhibiting human immunodeficiency virus (HIV) protease. The present invention also relates to compositions and methods for treating a retroviral infection and in particular an HIV infection, and to processes for making such compounds and synthetic intermediates employed in these processes.



Inventors:
Chen, Xiaoqi (San Mateo, CA, US)
Kempf, Dale J. (Libertyville, IL, US)
Norbeck, Daniel W. (Grayslake, IL, US)
Mohammadi, Fariborz (Lake Forest, IL, US)
Application Number:
09/777282
Publication Date:
07/19/2001
Filing Date:
02/06/2001
Assignee:
CHEN XIAOQI
KEMPF DALE J.
NORBECK DANIEL W.
MOHAMMADI FARIBORZ
Primary Class:
Other Classes:
514/326, 544/363, 544/369, 546/139, 546/209
International Classes:
C07D277/24; C07D417/12; C07D417/14; (IPC1-7): A61K31/497; A61K31/445; C07D401/00; C07D417/00
View Patent Images:



Primary Examiner:
PATEL, SUDHAKER B
Attorney, Agent or Firm:
Steven F. Weinstock (Abbott Laboratories Department 377 / AP6D-2 100 Abbott Park Road, Abbott Park, IL, 60064-6050, US)
Claims:
1. A compound having formula I: 21 wherein R1 is a thiazolyl group having the formula 22 and R2 is a group having the formula: 23 wherein R3 is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl; and Y is CH or N; R4 is —W—R5; W is —O—, —S—, or —(CH2)n—; and R5 is selected from the group consisting of alkyl, and aryl; n is from 0 to 6; or R4 and the ring taken together can form a bicyclic group having the formula: 24 with the proviso that when W is O, or S then Y is CH; R6 is hydrogen, alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl,or (heteroaryl)alkyl; and Z is —O—, —S—, —CH2— or —N(R7)—; and R7 is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl; wherein the alkyl, aryl, heterocyclic, and heteroaryl groups can be optionally substituted with 1 to 5 substituents selected from the group consisting of hydroxy, alkoxy, amino, alkylamino, dialkylamino and halogen; or a pharmaceutically acceptable salt, ester or prodrug thereof.

2. The compound according to claim 1, wherein R3 is alkyl or cycloalkyl and Z is —O—, or —N(R7)—.

3. The compound according to claim 2, wherein R3 is alkyl.

4. The compound according to claim 2, wherein R3 is cycloalkyl selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

5. The compound according to claim 3, wherein R3 is selected from the group consisting of hydrogen, methyl, ethyl, propyl and isopropyl.

6. The compound according to claim 5, wherein R3 is isopropyl.

7. The compound according to claim 1, wherein Z is —O—.

8. The compound according to claim 1, wherein Z is —N(R7)— and R7 is methyl.

9. The compound according to claim 1, wherein Y is nitrogen, W is —CH2— and R5 is aryl selected from the group consisting of phenyl, methylenedioxyphenyl, and heteroaryl.

10. The compound according to claim 9, wherein R5 is substituted with fluorine.

11. The compound according to claim 9, wherein R5 is substituted with 1 to 3 hydroxy groups.

12. The compound according to claim 9, wherein R5 is substituted with 1 to 3 alkoxy or alkylthio groups.

13. The compound according to claim 9, wherein R5 is substituted with two alkoxy groups.

14. The compound according to claim 9, wherein R5 is substituted with at least one hydroxy group and at least one methoxy group.

15. The compound according to claim 1, wherein Y is CH, and R5 is alkyl, or aryl selected from the group consisting of phenyl, methylenedioxyphenyl, and heteroaryl.

16. The compound according to claim 15, wherein R5 is substituted with 1 to 3 alkoxy or alkylthio groups.

17. The compound according to claim 15, wherein R5 is substituted with a hydroxy group.

18. The compound according to claim 15, wherein R5 is substituted with a methoxy group.

19. The compound according to claim 15, wherein W is —O—.

20. The compound according to claim 19, wherein R5 is methyl substituted with a thiazolyl group.

21. The compound according to claim 1, wherein R6 is selected from the group consisting of alkyl, hydroxyalkyl, and cycloalkyl.

22. The compound according to claim 21, wherein R6 is lower alkyl selected from the group consisting of methyl, ethyl, propyl, or butyl.

23. The compound according to claim 22, wherein R6 is tert-butyl or hydroxybutyl.

24. A compound having formula II: 25 wherein R1 is a thiazolyl group having the formula 26 and R2 is a group having the formula: 27 wherein R3 is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl; and X is —C(O)— or —S(O)2—; R8 is alkyl, aryl, (aryl)alkyl, alkylamino, dialkylamino, heterocyclic, (heterocyclic)alkyl, heteroaryl,or (heteroaryl)alkyl; R9 is alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl; and Z is —O—, —S—, —CH2— or —N(R7)— wherein R7 is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, heteroaryl, or (heteroaryl)alkyl; wherein the alkyl, aryl, heterocyclic, and heteroaryl groups can be optionally substituted with 1 to 5 substituents selected from the group consisting of hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen; or a pharmaceutically acceptable salt, ester or prodrug thereof.

25. The compound according to claim 24, wherein R3 is alkyl or cycloalkyl and Z is —O—, or —N(R7)—.

26. The compound according to claim 25, wherein R3 is alkyl.

27. The compound according to claim 25, wherein R3 is cycloalkyl selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.

28. The compound according to claim 26, wherein R3 is selected from the group consisting of hydrogen, methyl, ethyl, or propyl.

29. The compound according to claim 27, wherein R3 is isopropyl.

30. The compound according to claim 25, wherein Z is —O—.

31. The compound according to claim 25, wherein Z is —N(R7)— and R7 is methyl.

32. The compound according to claim 24, wherein R8 is selected from the group consisting of alkyl, aryl, (aryl)alkyl, alkylamino, dialkylamino, or heteroaryl.

33. The compound according to claim 24, wherein X is —S(O)2—; R8 is aryl selected from the group consisting of phenyl, and heteroaryl; and R9 is lower alkyl.

34. The compound according to claim 24, wherein R9 is selected from the group consisting of isopropyl, isobutyl, or 3-methyl-1-butyl.

35. The compound according to claim 34, wherein R9 is iso-butyl.

36. The compound according to claim 32, wherein R8 is substituted with 1 to 3 amino groups.

37. The compound selected from the group consisting of 2-(1-methylethyl)-4-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-4-(1,3-benzodioxol-5-ylmethyl)-2-(((1,1-dimethylethyl)amino)carbonyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-methylethyl)-4-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(phenylmethyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-methylethyl)-4-thiazolylmethyl-(1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-((4-fluorophenyl)methyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-methylethyl)-4-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(5-thienylmethyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-methylethyl)-4-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(4-(3-hydroxyphenyl)methyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-methylethyl)-4-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(3-pyridinylmethyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-methylethyl)-4-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(4-pyridinylmethyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-methylethyl)-4-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-((4-hydroxyphenyl)methyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-methylethyl)-4-thiazolylmethyl-(1S)-1-((((1S,2R)-3-((2S)-4-(1H-benzimidiazol-2-ylmethyl)-2-(((1,1-dimethylethyl)amino)carbonyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-methylethyl)-4-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(2-quinolinylmethyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-methylethyl)-4-thiazolylmethyl ((1S)-1-((((1S,2R)-3-((2S)-4-((3,4-dimethoxylphenyl)methyl)-2-(((1,1-dimethylethyl)amino)carbonyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; N′-((1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(5-thiazolylmethyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)-N-methyl-N-(2-(1-methylethyl)-4-thiazolylmethyl)urea; N′-((1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(phenylmethyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)-N-methyl-N-(2-(1-methylethyl)-4-thiazolylmethyl)urea; N′-((1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-((4-hydroxy-3-methoxyphenyl)methyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)-N-methyl-N-(2-(1-methylethyl)-4-thiazolylmethyl)urea; 2-methyl-4-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-4-(1,3-benzodioxol-5-ylmethyl)-2-(((1,1-dimethylethyl)amino)carbonyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-methyl-4-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-4-((3,4-dimethoxylphenyl)methyl)-2-(((1,1-dimethylethyl)amino)carbonyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-methyl-4-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-((4-fluorophenyl)methyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-ethyl-4-thiazolylmethyl-(1S)-1-((((1S,2R)-3-((2S)-4-((3,4-dimethoxylphenyl)methyl)-2-(((1,1-dimethylethyl)amino)carbonyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-methylethyl)-5-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-4-((3,4-dimethoxylphenyl)methyl)-2-(((1,1-dimethylethyl)amino)carbonyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-ethyl-5-thiazolylmethyl-(1S)-1-((((1S,2R)-3-((2S)-4-((3,4-dimethoxylphenyl)methyl)-2-(((1,1-dimethylethyl)amino)carbonyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-methyl-5-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-4-((3,4-dimethoxylphenyl)methyl)-2-(((1,1-dimethylethyl)amino)carbonyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; N′-((1R)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(5-thiazolylmethyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)-N-methyl-N-(5-thiazolylmethyl)urea; N′-((1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(5-thiazolylmethyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)-N-methyl-N-(5-thiazolylmethyl)urea; 5-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(phenylmethyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 5-thiazolylmethyl-(1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(5-thiazolylmethyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; N′-((1S)-1-((((1S,2R)-3-((((1,1-dimethylethyl)amino)carbonyl)(2-methylethyl)propylamino)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)-N-methyl-N-(2-(1-methylethyl)-4-thiazolylmethyl)urea; 2-(1-methylethyl)-4-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((((1,1-dimethylethyl)amino)carbonyl)(2-methylethyl)propylamino)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-methylethyl)-4-thiazolylmethyl-(1S)-1-((((1S,2R)-3-(((4-aminophenyl)sulfonyl)(1-methylethyl)amino)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; N′-((1S)-1-((((1S,2R)-3-(((4-aminophenyl)sulfonyl)(1-methylethyl)amino)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)-N-methyl-N-(2-(1-methylethyl)-4-thiazolylmethyl)urea; 2-(1-methylethyl)-5-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((3S)-3-(((1,1-dimethylethyl)amino)carbonyl)(4aα,8aα)octahydro-2-isoquinolinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; N′-((1S)-1-((((1S,2R)-3-((3S)-3-(((1,1-dimethylethyl)amino)carbonyl)(4aα,8aα)octahydro-2-isoquinolinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)-N-methyl-N-(2-(1-methylethyl)-5-thiazolylmethyl)urea, N′-((1S)-1-((((1S,2R)-3-((2S,4R)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(5-thiazolylmethoxy)-1-piperidinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)-N-methyl-N-(2-(1-methylethyl)-4-thiazolylmethyl)urea; 2-(1-methylethyl)-4-thiazolylmethyl ((1S)-1-((((1S,2R)-3-((2S,4R)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(5-thiazolylmethoxy)-1-piperidinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; S-(2-(1-methylethyl)-4-thiazolylmethyl) ((1S)-1-((((1S,2R)-3-((2S)-4-(1,3-benzodioxol-5-ylmethyl)-2-(((1,1-dimethylethyl)amino)carbonyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamothioate; and 4-(1,3-benzodioxol-5-ylmethyl)-N-(1,1-dimethylethyl)-1-((2R,3S)-3-(((2S)-2-((3-(2-(1-methylethyl)-4-thiazolyl)-1-oxopropyl)amino)-3-methyl-1-oxobutyl)amino)-2-hydroxy-4-phenylbutyl)-2-piperazinecarboxamide; or a pharmaceutically acceptable salt, ester or prodrug thereof.

38. The compound according to claim 37, selected from the group consisting of: 2-(1-Methylethyl)-4-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-4-(1,3-benzodioxol-5-ylmethyl)-2-(((1,1-dimethylethyl)amino)carbonyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-Methylethyl)-4-thiazolylmethyl-(1S)-1-((((1S,2R)-3-(((4-aminophenyl)sulfonyl)(1-methylethyl)amino)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-Methylethyl)-4-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((2S)-2-(((1,1-dimethylethyl)amino)carbonyl)-4-(5-thienylmethyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; 2-(1-Methylethyl)-4-thiazolylmethyl ((1S)-1-((((1S,2R)-3-((2S)-4-((3,4-dimethoxylphenyl)methyl)-2-(((1,1-dimethylethyl)amino)carbonyl)-1-piperazinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; N′-((1S)-1-((((1S,2R)-3-((2S,4R)-2-(((1,1-Dimethylethyl)amino)carbonyl)-4-(5-thiazolylmethoxy)-1-piperidinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)-N-methyl-N-(2-(1-methylethyl)-4-thiazolylmethyl)urea; and 2-(1-Methylethyl)-5-thiazolylmethyl-((1S)-1-((((1S,2R)-3-((3S)-3-(((1,1-dimethylethyl)amino)carbonyl)(4aα,8aα)octahydro-2-isoquinolinyl)-2-hydroxy-1-(phenylmethyl)propyl)amino)carbonyl)-2-methylpropyl)carbamate; or a pharmaceutically acceptable salt, ester or prodrug thereof.

39. A pharmaceutical composition for treating an HIV infection comprising a pharmaceutical carrier and a therapeutically effective amount of a compound of claim 1.

40. A pharmaceutical composition for treating an HIV infection comprising a pharmaceutical carrier and a therapeutically effective amount of a compound of claim 24.

41. A method for inhibiting HIV protease comprising administering to a mammal in need of such treatment a therapeutically effective amount of a compound of claim 1.

42. A method for treating an HIV infection comprising administering to a mammal in need of such treatment a therapeutically effective amount of a compound of claim 24.

43. A pharmaceutical composition for treating an HIV infection comprising a pharmaceutical carrier and a therapeutically effective amount of a compound of claim 9.

44. A pharmaceutical composition for treating an HIV infection comprising a pharmaceutical carrier and a therapeutically effective amount of a compound of claim 20.

45. A pharmaceutical composition for treating an HIV infection comprising a pharmaceutical carrier and a therapeutically effective amount of a compound of claim 36.

46. A process for the preparation of a compound of the formula I: 28 wherein R1 is a thiazolyl group having the formula 29 and R2 is a group having the formula: 30 wherein R3 is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl; and Y is CH or N; R4 is —W—R5; W is —O—, —S—, or —(CH2)n—; and R5 is selected from the group consisting of alkyl, and aryl; n is from 0 to 6; or R4 and the ring taken together can form a bicyclic group having the formula: 31 with the proviso that when W is O, or S then Y is CH; R6 is hydrogen, alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, (heterocyclic)alkyl, heteroaryl,or (heteroaryl)alkyl; and Z is —O—, —S—, —CH2— or —N(R7)—; and R7 is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, heteroaryl, or (heteroaryl)alkyl; wherein the alkyl, aryl, heterocyclic, and heteroaryl groups can be optionally substituted with 1 to 5 substituents selected from the group consisting of hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen.

47. A process for the preparation of a compound of the formula II: 32 wherein R1 is a thiazolyl group having the formula 33 and R2 is a group having the formula: 34 wherein R3 is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl; and X is —C(O)— or —S(O)2—; R8 is alkyl, aryl, (aryl)alkyl, alkylamino, dialkylamino, heterocyclic, (heterocyclic)alkyl, heteroaryl,or (heteroaryl)alkyl; R9 is alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl; and Z is —O—, —S—, —CH2— or —N(R7)— wherein R7 is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl; wherein the alkyl, aryl, heterocyclic, and heteroaryl groups can be optionally substituted with 1 to 5 substituents selected from the group consisting of hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen.

Description:

[0001] This application claims the benefit of U.S Provisional Application for Patent No. 60/085,709, filed May 15, 1998.

TECHNICAL FIELD

[0002] The present invention relates to novel compounds compositions and methods for inhibiting retroviral proteases and in particular for inhibiting human immunodeficiency virus (HIV) protease. The present invention also relates to compositions and methods for treating a retroviral infection and in particular an HIV infection, and to processes for making such compounds and synthetic intermediates employed in these processes.

BACKGROUND OF THE INVENTION

[0003] Retroviruses are those viruses which utilize a ribonucleic acid (RNA) intermediate and a RNA-dependent deoxyribonucleic acid (DNA) polymerase, reverse transcriptase, during their life cycle. Retroviruses include, but are not limited to, the RNA viruses of the Retroviridae family, and also the DNA viruses of the Hepadnavirus and Caulimovirus families. Retroviruses cause a variety of disease states in man, animals and plants. Some of the more important retroviruses from a pathological standpoint include human immunodeficiency viruses (HIV-1 and HIV-2), which cause acquired immune deficiency syndrome (AIDS) in man, hepatitis B virus, which causes hepatitis and hepatic carcinomas in man, human T-cell lymphotrophic viruses I, II, IV and V, which cause human acute cell leukemia, and bovine and feline leukemia viruses which cause leukemia in domestic animals.

[0004] Proteases are enzymes which cleave proteins at specific peptide bonds. Many biological functions are controlled or mediated by proteases and their complementary protease inhibitors. For example, the protease renin cleaves the peptide angiotensinogen to produce the peptide angiotensin I. Angiotensin I is further cleaved by the protease angiotensin converting enzyme (ACE) to form the hypotensive peptide angiotensin II. Inhibitors of renin and ACE are known to reduce high blood pressure in vivo. An inhibitor of a retroviral protease will provide a therapeutic agent for diseases caused by the retrovirus.

[0005] The genomes of retroviruses encode a protease that is responsible for the proteolytic processing of one or more polyprotein precursors such as the pol and gag gene products. See Wellink, Arch. Virol. 98 1 (1988). Retroviral proteases most commonly process the gag precursor into core proteins, and also process the pol precursor into reverse transciptase and retroviral protease. In addition, retroviral proteases are sequence specific. See Pearl, Nature 328 482 (1987).

[0006] The correct processing of the precursor polyproteins by the retroviral protease is necessary for the assembly of infectious virions. It has been shown that in vitro mutagenesis that produces protease-defective virus leads to the production of immature core forms which lack infectivity. See Crawford, J. Virol. 53 899 (1985); Katoh, et al., Virology 145 280 (1985). Therefore, retroviral protease inhibition provides an attractive target for antiviral therapy. See Mitsuya, Nature 325 775 (1987).

[0007] Current treatments for viral diseases usually involve administration of compounds that inhibit viral DNA synthesis. Current treatments for AIDS involve administration of compounds such as 3′-azido-3′-deoxythymidine (AZT), 2′,3′-dideoxycytidine (DDC) and 2′,3′-dideoxyinosine (DDI) and compounds which treat the opportunistic infections caused by the immunosuppression resulting from HIV infection. None of the current AIDS treatments have proven to be totally effective in treating and/or reversing the disease. In addition, many of the compounds currently used to treat AIDS cause adverse side effects including low platelet count, renal toxicity and bone marrow cytopenia.

[0008] Recently the HIV protease inhibitors ritonavir, saquinavir, nelfinavir, and indinavir have been approved in the U.S. for treatment of HIV infections. However, there is a continuing need for improved HIV protease inhibitors.

SUMMARY OF THE INVENTION

[0009] The present invention comprises retroviral protease inhibiting compounds having formula I: 1

[0010] wherein R1 is a thiazolyl group having the formula 2

[0011] and R2 is a group having the formula: 3

[0012] wherein R4 group is —WR5.

[0013] The R3 group is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl, and Y is CH or N. W is selected from the group consisting of —O—, —S—, or —(CH2)n—, where n is from 0 to 6, with the proviso that when W is O, or S then Y is CH. R5 is selected from the group consisting of alkyl, and aryl. Optionally, R4 and the ring to which it is attached, taken together can form a bicyclic group having the formula: 4

[0014] The R6 group is hydrogen, alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl, Z is —O—, —S—, —CH2— or —N(R7)—; and R7 is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl.

[0015] The present invention also comprises retroviral protease inhibiting compounds having formula II: 5

[0016] wherein R1 is a thiazolyl group having the formula 6

[0017] and R2 is a group having the formula: 7

[0018] wherein X is —C(O)— or —S(O)2— and R8 is alkyl, aryl, (aryl)alkyl, alkylamino, dialkylamino, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl.

[0019] R3 is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl and R9 is alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl. The Z group is —O—, —S—, —CH2— or —N(R7)—, and R7 is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl.

[0020] The alkyl, aryl, heterocyclic, and heteroaryl groups in the compounds of the invention can be optionally substituted with from 1 to 5 substituents and preferrably from 1 to 3 substituents. The substituents are selected from the group consisting of hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen. The invention also includes pharmaceutically acceptable salts, esters or prodrugs of compounds I and II.

DETAILED DESCRIPTION OF THE INVENTION

[0021] All patents, patent applications, and literature references cited in the specification are hereby incorporated by reference in their entirety. In the case of inconsistencies, the present disclosure, including definitions, will prevail.

[0022] The present invention comprises retroviral protease inhibiting compounds having formula I: 8

[0023] wherein R1 is a thiazolyl group having the formula 9

[0024] and R2 is a group having the formula: 10

[0025] wherein R4 group is —WR5.

[0026] The R3 group is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl, and Y is CH or N. W is selected from the group consisting of —O—, —S—, or —(CH2)n—, where n is from 0 to 6, with the proviso that when W is O, or S then Y is CH. R5 is selected from the group consisting of alkyl, and aryl. Optionally, R4 and the ring to which it is attached, taken together can form a bicyclic group having the formula: 11

[0027] with the proviso that when W is O, or S then Y is CH. The R6 group is hydrogen, alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl, Z is —O—, —S—, —CH2— or —N(R7)—; and R7 is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl.

[0028] The present invention also comprises retroviral protease inhibiting compounds having formula II: 12

[0029] wherein R1 is a thiazolyl group having the formula: 13

[0030] and R2 is a group having the formula: 14

[0031] wherein X is —C(O)— or —S(O)2— and R8 is alkyl, aryl, (aryl)alkyl, alkylamino, dialkylamino, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl.

[0032] R3 is selected from the group consisting of hydrogen, alkyl, amino, alkylamino, dialkylamino and cycloalkyl and R9 is alkyl, cycloalkyl, aryl, (aryl)alkyl, heterocyclic, heteroaryl, or (heteroaryl)alkyl. The Z group is —O—, —S—, —CH2— or —N(R7)—, and R7 is hydrogen, alkyl, aryl, (aryl)alkyl, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl.

[0033] The alkyl, aryl, heteroaryl, and heterocyclic groups of the compounds of the invention, having formula I or II, can optionally be substituted with from 1 to 5 substituents and preferrably from 1 to 3 substituents. The substituents are selected from the group consisting of hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen. The invention also includes pharmaceutically acceptable salts, esters or prodrugs of compounds I and II.

[0034] In the compounds of the invention, a preferred R3 group is alkyl or cycloalkyl. More preferred are compounds where R3 is alkyl selected from the group consisting of methyl, ethyl, or propyl or cycloalkyl selected from the group consisting of cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl. The most preferred R3 group is isopropyl.

[0035] The preferred Z groups are —O—, or —N(R7)—. When Z is —N(R7)— a preferred R7 is methyl.

[0036] In compounds having formula I, and R2 is a group having the formula: 15

[0037] wherein Y is CH or N and R4 is —W—R5, the preferred R5 group is aryl selected from the group consisting of phenyl, methylenedioxyphenyl, and heteroaryl. In compounds where Y is nitrogen, W is —(CH2)n—, R5 is alkyl or aryl and n is from 0 to 6.

[0038] In compounds where Y is CH, a preferred W is —O—, and preferably, R5 is alkyl, or aryl selected from the group consisting of phenyl, methylenedioxyphenyl, and heteroaryl.

[0039] The alkyl, aryl, heterocyclic, and heteroaryl groups can be substituted with from 1 to 5 substituents and preferrably from 1 to 3 substituents.

[0040] Examples of substituents, for the alkyl, aryl, heterocyclic, and heteroaryl groups, are selected from the group consisting of hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen. Preferred substituents are fluorine, hydroxy, alkoxy, or alkylthio groups. The preferred alkoxy is methoxy. The preferred halogen is fluorine

[0041] In a preferred embodiment the alkyl or aryl groups can be substituted with one to three groups. The preferred substituents are hydroxy, methoxy or fluorine. The preferred aryl groups include phenyl, such as, for example, methylenedioxyphenyl, and heteroaryl such as, for example, furanyl, thienyl, benzothienyl, thiazolyl and the like. A preferred heteroaryl group is thiazolyl. In a preferred compound n is zero, and R5 is methyl substituted with a thiazolyl group.

[0042] The R6 group is selected from the group consisting of alkyl, hydroxyalkyl, and cycloalkyl. Preferably R6 is lower alkyl group such as, for example, methyl, ethyl, propyl, butyl and the like. A preferred R6 group is tert-butyl or hydroxy-butyl.

[0043] The R8 group is selected from the group consisting of alkyl, aryl, (aryl)alkyl, alkylamino, dialkylamino, heterocyclic, (heterocyclic)alkyl, heteroaryl, or (heteroaryl)alkyl. A preferred R8 group is (alkyl)amino, such as, for example, (tertbutyl)amino.

[0044] The preferred R9 group is a lower alkyl group such as, for example propyl, butyl, pentyl and the like. More preferred R9 groups are isopropyl, tert-butyl, isobutyl, 3-methyl-1-butyl, and the like. Most preferred is the iso-butyl group.

[0045] In a preferred compound of the invention X is —S(O)2—, R8 is aryl selected from the group consisting of phenyl, and heteroaryl and R9 is iso-butyl.

[0046] In the compounds of the invention, combinations of substituents and/or variables are permissible only if such combinations result in stable compounds. As used herein, the term “stable compound” refers to a compound that is sufficiently stable to survive isolation to a useful degree of purity from a reaction mixture and formulation into a therapeutic dosage form suitable for administration.

[0047] Preferred compounds of the invention are selected from the group consisting of:

[0048] 2-(1-methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R )-3-[(2S)-4-(1,3-benzodioxol-5-ylmethyl)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbon yl]-2-methylpropyl]carbamate;

[0049] 2-(1-methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[-(1,1-dimethylethyl)amino]carbonyl]-4-(phenylmethyl)-1-piperazinyl]-2-hyd roxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0050] 2-(1-methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-[(4-fluorophenyl)methyl]-1-piperaziny l]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0051] 2-(1-methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(5-thienylmethyl)-1-piperazinyl]-2-h ydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0052] 2-(1-methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-[4-(3-hydroxyphenyl)methyl]-1-pipera zinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0053] 2-(1-methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(3-pyridinylmethyl)-1-piperazinyl]-2 -hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0054] 2-(1-methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(4-pyridinylmethyl)-1-piperazinyl]-2 -hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0055] 2-(1-methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-[(4-hydroxyphenyl)methyl]-1-piperazi nyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0056] 2-(1-methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1H-benzimidiazol-2-ylmethyl)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piper azinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0057] 2-(1-methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(2-quinolinylmethyl)-1-piperazinyl]- 2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0058] 2-(1-methylethyl)-4-thiazolylmethyl [(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethyl) propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0059] N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]a mino]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea;

[0060] N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(phenylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino] carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea;

[0061] N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-[4-hydroxy-3-methoxyphenyl)methyl]-1-piperazinyl]-2-hydroxy-1-(phenyl methyl)propyl]amino]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]-urea;

[0062] 2-methyl-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,3-benzodioxol-5-ylmethyl)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2 -hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0063] 2-methyl-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl] -2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0064] 2-methyl-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-[(4-fluorophenyl)methyl]-1-piperazinyl]-2-hyd roxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0065] 2-ethyl-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2 -hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0066] 2-(1-methylethyl)-5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-pip erazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0067] 2-ethyl-5-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-(dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]- 2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0068] 2-methyl-5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl] -2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0069] N′-[(1R)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]a mino]carbonyl]-2-methylpropyl-9 -N-methyl-N-(5-thiazolylmethyl)urea;

[0070] N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]a mino]carbonyl]-2-methylpropyl]-N-methyl-N-(5-thiazolylmethyl)urea;

[0071] 5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(phenylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylmethy l)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0072] 5-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylm ethyl)propyl]amino]carbonyl]-2-methylpropyl]-carbamate;

[0073] N′-[(1S)-1-[[[(1S,2R)-3-[[[(1,1-dimethylethyl)amino]carbonyl](2-methylethyl)propylamino]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2- methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea;

[0074] 2-(1-methylethyl)-4-thiazolylmnethyl-[(1S)-1-[[[(1S,2R)-3-[[[(1,1-dimethylethyl)amino]carbonyl](2-methylethyl)propylamino]-2-hydroxy-1-(phen ylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0075] 2-(1-methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[[(4-aminophenyl)sulfonyl](1-methylethyl)amino]-2-hydroxy-1-(phenylmethyl)propyl]ami no]carbonyl]-2-methylpropyl]carbamate;

[0076] N′-[(1S)-1-[[[(1S,2R)-3-[[(4-aminophenyl)sulfonyl](1-methylethyl)amino]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]-N-m ethyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea;

[0077] 2-(1-methylethyl)-5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(3S)-3-[[(1,1-dimethylethyl)amino]carbonyl](4aα,8aα)octahydro-2-isoquinolinyl]-2-h ydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0078] N′-[(1S)-1-[[[(1S,2R)-3-[(3S)-3-[[(1,1-dimethylethyl)amino]carbonyl](4aα,8aα)octahydro-2-isoquinolinyl]-2-hydroxy-1-(phenylmethyl)propyl]ami no]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-5-thiazolylmethyl]urea,

[0079] N′-[(1S)-1-[[[(1S,2R)-3-[(2S,4R)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethoxy)-1-piperidinyl]-2-hydroxy-1-(phenylmethyl)prop yl]amino]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea;

[0080] 2-(1-methylethyl)-4-thiazolylmethyl [(1S)-1-[[[(1S,2R)-3-[(2S,4R)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethoxy)-1-piperidinyl]-2-hydroxy-1-(phenylmethyl)propyl] amino]carbonyl]-2-methylpropyl]carbamate;

[0081] S-[2-(1-methylethyl)-4-thiazolylmethyl] [(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,3-benzodioxol-5-ylmethyl)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)pr opyl]amino]carbonyl]-2-methylpropyl]-carbamothioate; and

[0082] 4-(1,3-benzodioxol-5-ylmethyl)-N-(1,1-dimethylethyl)-1-[(2R,3S)-3-[[(2S)-2-[[3-[2-(1-methylethyl)-4-thiazolyl]-1-oxopropyl]amino]-3-methyl-1 -oxobutyl]amino]-2-hydroxy-4-phenylbutyl]-2-piperazinecarboxamide;

[0083] or a pharmaceutically acceptable salt, ester or prodrug thereof.

[0084] Most preferred compounds of the invention are selected from the group consisting of:

[0085] 2-(1-Methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,3-benzodioxol-5-ylmethyl)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piper azinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0086] 2-(1-Methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[[(4-aminophenyl)sulfonyl](1-methylethyl)amino]-2-hydroxy-1-(phenylmethyl)propyl]ami no]carbonyl]-2-methylpropyl]carbamate;

[0087] 2-(1-Methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(5-thienylmethyl)-1-piperazinyl]-2-h ydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0088] 2-(1-Methylethyl)-4-thiazolylmethyl [(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethyl) propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0089] N′-[(1S)-1[[[(1S,2R)-3-[(2S,4R)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethoxy)-1-piperidinyl]-2-hydroxy-1-(phenylmethyl)propy l]amino]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea; and

[0090] 2-(1-Methylethyl)-5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(3S)-3-[[(1,1-dimethylethyl)amino]carbonyl](4aα,8aα)octahydro-2-isoquinolinyl]-2-h ydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate;

[0091] or a pharmaceutically acceptable salt, ester or prodrug thereof.

[0092] The term “alkyl” as used herein refers to straight or branched chain alkyl radicals containing from 1 to 12 carbon atoms. The term “lower alkyl” refers to straight or branched chain alkyl radicals containing from 1 to 6 carbon atoms including, but not limited to, methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, t-butyl n-pentyl, 1-methylbutyl, 2,2-dimethylbutyl, 2-methylpentyl, 2,2-dimethylpropyl, n-hexyl, and the like. The alkyl groups can be unsubstituted or substituted with from one to five substituents independently selected from hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen. The alkyl groups can be optionally interrupted by one or more heteroatoms selected from the group consisting of oxygen, nitrogen, sulfur, and phosphorous.

[0093] The term “cycloalkyl” as used herein refers to an aliphatic ring system having 3 to 8 carbon atoms including, but not limited to, cyclopropyl, cyclopentyl, cyclohexyl, and the like.

[0094] The term “alkoxy” as used herein refers to groups having the formula —OR10 wherein R10 is a lower alkyl group.

[0095] The term “thioalkyl” as used herein refers to groups having the formula —SR11 wherein R11 is a lower alkyl group.

[0096] The term “alkylamino” as used herein refers to groups having the formula —NHR12 wherein R12 is a lower alkyl group.

[0097] The term “dialkylamino” as used herein refers to groups having the formula —N(R13)2 wherein each R13 is independently a lower alkyl group.

[0098] The term “halo or halogen” as used herein refers to F, Cl, Br or I.

[0099] The term “(halo)alkyl” as used herein refers to a lower alkyl group in which one or more hydrogen atoms has been replaced with a halogen including, but not limited to, trifluoromethyl, trichloromethyl, difuoromethyl, dichloromethyl, fluoromethyl, chloromethyl, chloroethyl, 2,2-dichloroethyl and the like.

[0100] The term “aryl” as used herein refers to a mono- or bicyclic carbocyclic ring system comprising 6 to 12 carbon atoms and having one or two aromatic rings including, but not limited to, phenyl, naphthyl, tetrahydronaphthyl, indanyl, indenyl and the like. Aryl groups can be unsubstituted or substituted with from one to five substituents independently selected from hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen.

[0101] The term “(aryl)alkyl” as used herein refers to an aryl group as previously defined, appended to a lower alkyl radical, for example, benzyl and the like.

[0102] The term “heterocyclic ring” or “heterocyclic” or “heterocycle” as used herein refers lo any 3- or 4-membered ring containing a heteroatom selected from oxygen, nitrogen and sulfur; or a 5-, 6- or 7-membered ring containing one, two or three heteroatoms independently selected from the group consisting of nitrogen, oxygen and sulfur or a 5-membered ring containing 4 nitrogen atoms; and includes a 5-, 6- or 7-membered ring containing one, two or three nitrogen atoms; one oxygen atom; one sulfur atom; one nitrogen and one sulfur atom; one nitrogen and one oxygen atom; two oxygen atoms in non-adjacent positions; one oxygen and one sulfur atom in non-adjacent positions; two sulfur atoms in non-adjacent positions; two sulfur atoms in adjacent positions and one nitrogen atom; two adjacent nitrogen atoms and one sulfur atom; two non-adjacent nitrogen atoms and one sulfur atom; two non-adjacent nitrogen atoms and one oxygen atom. The 5-membered ring has 0-2 double bonds and the 6- and 7-membered rings have 0-3 double bonds. The nitrogen heteroatoms can be optionally quaternized. The term “heterocyclic” also includes bicyclic groups in which any of the above heterocyclic rings is fused to a benzene ring or a cyclohexane ring or another heterocyclic ring (for example, indolyl, quinolyl, isoquinolyl, tetrahydroquinolyl, benzofuryl, bistetrahydrofuranyl or benzothienyl and the like). Heterocyclics include: azetidinyl, pyrrolyl, pyrrolinyl, pyrrolidinyl, pyrazolyl, pyrazolinyl, pyrazolidinyl, imidazolyl, imidazolinyl, imidazolidinyl, pyridyl, piperidinyl, homopiperidinyl, pyrazinyl, piperazinyl, pyrimidinyl, pyridazinyl, oxazolyl, oxazolidinyl, isoxazolyl, isoxazolidinyl, morpholinyl, thiazolyl, thiazolidinyl, isothiazolyl, isothiazolidinyl, indolyl, quinolinyl, isoquinolinyl, benzimidazolyl, benzothiazolyl, benzoxazolyl, furyl, thienyl, tetrahydrofuranyl, tetrahydrothienyl, thiazolidinyl, isothiazolyl, triazolyl, tetrazolyl, isoxazolyl, oxadiazolyl, thiadiazolyl, pyrrolyl, pyrimidyl and benzothienyl. Heterocyclics also include compounds of the formula: 16

[0103] wherein X* is —CH2—, —NH— or —O—, Y* is —C(O)— or [—C(R″)2—]v wherein R″ is hydrogen or C1-C4-alkyl and v is 1, 2 or 3 and Z* is —O— or —NH—; such as 1,3-benzodioxolyl, 1,4-benzodioxanyl and the like.

[0104] The term “methylenedioxyphenyl” refers to a substituent having the formula: 17

[0105] Heterocyclic groups can be unsubstituted or substituted with from one to five substituents independently selected from hydroxy, alkoxy, alkylthio, amino, alkylamino, dialkylamino and halogen. In addition, nitrogen containing heterocycles can be N-protected.

[0106] The term “(heterocyclic)alkyl” as used herein refers to a heterocyclic group appended to a lower alkyl radical including, but not limited to, pyrrolidinylmethyl, morpholinylmethyl and the like.

[0107] The term “heteroaryl” as used herein refers to an heterocyclic group containing at least one aromatic ring. Examples include groups such as, for example, furanyl, thienyl, benzothienyl, thiazolyl and the like.

[0108] The term “(heteroaryl)alkyl” as used herein refers to a heteroaryl group appended to a lower alkyl radical including, but not limited to, thienylmethyl, thienylethyl, furanylmethyl, and the like.

[0109] The term “N-protecting group” or “N-protected” as used herein refers to those groups intended to protect the N-terminus of an amino acid or peptide or to protect an amino group against undesirable reactions during synthetic procedures. Commonly used N-protecting groups are disclosed in T. H. Greene and P. G. M. Wuts, Protective Groups in Organic Synthesis. 2nd edition, John Wiley & Sons, New York (1991). N-protecting groups comprise acyl groups such as formyl, acetyl, propionyl, pivaloyl, t-butylacetyl, 2-chloroacetyl, 2-bromoacetyl, trifluoroacetyl, trichloroacetyl, phthalyl, o-nitrophenoxyacetyl, α-chlorobutyryl, benzoyl, 4-chlorobenzoyl, 4-bromobenzoyl, 4-nitrobenzoyl, and the like; sulfonyl groups such as benzenesulfonyl, p-toluenesulfonyl and the like; carbamate forming groups such as benzyloxycarbonyl, p-chlorobenzyloxycarbonyl, p-methoxybenzyloxycarbonyl, p-nitrobenzyloxycarbonyl, 2-nitrobenzyloxycarbonyl, p-bromobenzyloxycarbonyl, 3,4-dimethoxybenzyloxycarbonyl, 3,5-dimethoxybenzyloxycarbonyl, 2,4-dimethoxybenzyloxycarbonyl, 4-methoxybenzyloxycarbonyl, 2-nitro-4,5-dimethoxybenzyloxycarbonyl, 3,4,5-trimethoxybenzyloxycarbonyl, 1-(p-biphenylyl)-1-methylethoxycarbonyl, α,α-dimethyl-3,5-dimethoxybenzyloxycarbonyl, benzhydryloxycarbonyl, t-butyloxycarbonyl, diisopropylmethoxycarbonyl, isopropyloxycarbonyl, ethoxycarbonyl, methoxycarbonyl, allyloxycarbonyl, 2,2,2-trichloroethoxycarbonyl, phenoxycarbcnyl, 4-nitro-phenoxycarbonyl, fluorenyl-9-methoxycarbonyl, cyclopentyloxycarbonyl, adamantyloxycarbonyl, cyclohexyloxycarbonyl, phenylthiocarbonyl and the like; alkyl groups such as benzyl, triphenylmethyl, benzyloxymethyl and the like; and silyl groups such as trimethylsilyl and the like. Preferred N-protecting groups are formyl, acetyl, benzoyl, pivaloyl, t-butylacetyl, phenylsulfonyl, benzyl, t-butyloxycarbonyl (Boc) and benzyloxycarbonyl (Cbz).

[0110] The term “activated carboxylic acid derivative” as used herein refers to acid halides such as acid chlorides, and activated esters including, but not limited to, formic and acetic acid derived anhydrides, anhydrides derived from alkoxycarbonyl halides such as isobutyloxycarbonylchloride and the like, N-hydroxysuccinimide derived esters, N-hydroxyphthalimide derived esters, N-hydroxybenzotriazole derived esters, N-hydroxy-5-norbornene-2,3-dicarboxamide derived esters, 2,4,5-trichlorophenol derived esters, thiophenol derived esters, propylphosphonic acid derived anhydrides and the like.

[0111] The term “leaving group” as used herein refers to a group which is easily displaced from the compound, such as, for example, a halide (for example, Cl, Br or I) or a sulfonate (for example, mesylate, tosylate, triflate and the like).

[0112] As used herein, the terms “S” and “R” configuration are as defined by the IUPAC 1974 Recommendations for Section E, Fundamental Stereochemistry, Pure Appl. Chem. (1976) 45, 13- 30.

[0113] The compounds of the invention can comprise asymmetrically substituted carbon atoms. As a result, all stereoisomers of the compounds of the invention are meant to be included in the invention, including racemic mixtures, mixtures of diastereomers, as well as single diastereomers of the compounds of the invention.

[0114] This invention is intended to encompass compounds having Formula I or Formula II when prepared by synthetic processes or by metabolic processes. Preparation of the compounds of the invention by metabolic processes include those occurring in the human or animal body (in vivo) or processes occurring in vitro.

[0115] The reagents required for the synthesis of the compounds of the invention are readily available from a number of commercial sources such as Aldrich Chemical Co. (Milwaukee, Wis., USA); Sigma Chemical Co. (St. Louis, Mo., USA); and Fluka Chemical Corp. (Ronkonkoma, N.Y., USA); Alfa Aesar (Ward Hill, Mass. 01835-9953); Eastman Chemical Company (Rochester, N.Y. 14652-3512); Lancaster Synthesis Inc. (Windham, N.H. 03087-9977); Spectrum Chemical Manufacturing Corp. (Janssen Chemical) (New Brunswick, N.J. 08901); Pfaltz and Bauer (Waterbury, Conn. 06708). Compounds which are not commercially available can be prepared by employing known methods from the chemical literature.

[0116] The compounds of the invention can be prepared as shown in Schemes I-III. As outlined in Scheme I, an N-protected (for example, with a benzyloxycarbonyl group) phenylalanylepoxide 2 is coupled with an N-protected (for example, N-Boc) piperazine compound, 1 (using, for example, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDAC),1-hydroxybenzotriazole (HOBT), and an amine, such as, triethyl amine, and the like, in an inert solvent, for example, tetrahydrofuran, methylene chloride, and the like). The protected carboxamide product, 3, is deprotected (for example, by treatment with hydrogen gas, and an hydrogenation catalyst). The amine carboxamide 3a, is then coupled with a carboxylic acid, 4, or an activated derivative thereof, using, for example, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDAC),1-hydroxybenzotriazole (HOBT), and triethyl amine in an inert solvent, for example, tetrahydrofuran, methylene chloride, and the like, to provide the N-protected piperazine, 5. The piperazine is N-deprotected, (with for example, trifluoroacetic acid (TFA), and the like) and alkylated (where X1 is a leaving group) to provide the final product, 6. 18

[0117] For piperidyl compounds, where Y is —CH—, the synthesis is outlined in Scheme II, shown below. A mono-protected piperidine carbamate, 7, is alkylated to provide compound 8. The alkylated carbamate is N-deprotected, (with, for example, trifluoroacetic acid, and the like), followed by coupling with the epoxide, 9, to provide carboxamide, 10. The carboxamide is N-deprotected to provide compound 10a. Compound 10a is coupled with a carboxylic acid, 4, or an activated derivative thereof, to provide the product, 11. 19

[0118] The synthesis of compounds of the invention having the —N(R9)—X—R8 group, is outlined in Scheme III., Urea, 12, prepared according to the description in J. Med. Chem., 1993, 36, 288-291, is coupled with a carboxylic acid, 4, or an activated derivative thereof, using, for example, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDAC),1-hydroxybenzotriazole (HOBT), and triethyl amine in an inert solvent, for example, tetrahydrofuran, methylene chloride, and the like, to provide the product 13. 20

[0119] The following examples will serve to further illustrate the preparation of the novel compounds of the invention.

EXAMPLE 1

2-(1-Methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,3-benzodioxol-5-ylmethyl)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-pi perazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0120] 1A. Benzyl-1-[(1S,2R)-3-[(2S)-4-(1,1-dimethylethyloxy(carbonyl))-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethyl )propyl]carbamate.

[0121] The title compound was prepared according to the method described in J. Med. Chem,1993, 36, 288-291, starting with 3.6 g (12 mmol) of N-benzyloxycarbonyl-phenylalanylepoxide and 3.4 g (12 mmol) of N-tert-butyl-4-(tert-butyloxycarbonyl)-piperazine -2(S)-carboxamide, prepared according to the method described in J. Med. Chem., 1994 37, 3443-3451.

[0122] 1B. 1-[(1S,2R)-3-[(2S)-4-(1,1-Dimethylethyloxy(carbonyl))-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl ]amine.

[0123] A solution of 2.1 g (3.6 mmol) of benzyl-1-[(1S,2R)-3-[(2S)-4-(1,1-dimethylethyloxy(carbonyl))-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethyl )propyl]carbamate in 20 ml of methanol was treated with hydrogen, at greater than 1 atmosphere, in the presence of Pd/C (50 mg; 10%), and stirred for 2 hours. The catalyst was removed via filtration over a celite pad and the solvent was evaporated at reduced pressure to provide the title compound as a yellow oil (yield: 1.6 g; 99%).

[0124] 1C. 2-(1-Methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,1-dimethylethyloxy(carbonyl))-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-pi perazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0125] A solution of 1.5 g (3.3 mmol) of 1-[(1S,2R)-3-[(2S)-4-(1,1-dimethylethyloxy(carbonyl))-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl ]amine. in 7 ml of tetrahydrofuran (THF) and 7 ml of methylene chloride was prepared and treated with 1.0 g (3.3 mmol) of [(2-isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine, 642 mg (3.3 mmol) of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDAC), 45 mg (0.33 mmol) of 1-hydroxybenzotriazole (HOBT), and 466 μl (3.3 mmol) of triethyl amine. The solution was stirred at room temperature for 6 hours, and concentrated in vacuo. The residue was taken up with ethyl acetate, washed with equal portions of 3 N HCl solution, 10% aqueous NaHCO3 (20 mL), saturated brine, and dried over MgSO4. The product was concentrated in vacuo and purified by chromatography on silica gel, using 5% methanol:methylene chloride, to provide the title compound (yield: 2.4 g, 98%).

[0126] MS: 731 (M+H)+.

[0127] 1H NMR (DMSO-d6) δ 1.08 (d, J=7 Hz, 6H), 2.78 (heptet, J=7 Hz, 1H), 9.06 (br s, 1H), 9.30 (br s, 1H).

[0128] 1D. 2-(1-Methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethy l)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0129] A solution of 2.4 g (3.2 mmol) of 2-(1-methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,1-dimethylethyloxy(carbonyl))-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-pi perazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate in 20 ml of dichloromethane was prepared and treated with 2 ml of trifluoroacetic acid. The reaction was monitored by TLC. After the reaction was completed, the solvent was removed at reduced pressure. The residue was dissolved in methylene chloride, washed with saturated aqueous NaHCO3 and saturated brine. The organic layer was dried over Na2SO4 and concentrated in vacuo to provide the title compound as a light yellow oil (yield: 2.0 g; 90%).

[0130] 1E. 2-(1-Methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,3-benzodioxol-5-ylmethyl)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piper azinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0131] A solution of 514 mg (0.82 mmol) of 2-(1-methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethy l)propyl]amino]carbonyl]-2-methylpropyl]carbamate in 5 ml of dimethylformamide (DMF) was prepared and treated with 140 mg (0.8 mmol) of 3,4-dioxomethylenebenzyl chloride followed by 284 μl (1.6 mmol) of N,N-diisopropyl ethyl amine, stirred at room temperature over night, and partitioned between ethyl acetate and saturated sodium bicarbonate solution. The organic layer was washed with brine, dried over sodium sulfate, and concentrated in vacuo. The residue was purified by chromatography on silica gel, using 5% methanol:methylene chloride, to provide the title compound (yield: 502 mg; 81%).

[0132] MS: 765 (M+H)+.

[0133] 1H NMR (CDCl3) δ 0.67 (d, J=6.3 Hz, 3H), 0.83 (d, J=6.3 Hz, 3H), 1.37 (s, 9H), 1.41 (t, J=7.5, 6H), 2.05 (hexlet, J=6.0 Hz, 1H), 2.26 (m, 1H), 2.41 (m, 1H), 2.58 (m, 2H), 2.73 (m, 3H), 2.83 (m, 2H), 2.90 (m, 1H), 3.33 (AB, J=15.0 Hz, 2H), 3.46 (s, 2H), 3.80 (m, 1H), 3.93 (m, 1H), 4.22 (septet, J=4.5 Hz, 1H), 5.08 (m, 1H), 5.17 (d, J=4.5 Hz, 2H), 5.97 (s, 2H), 6.13 (d, J=9.0 Hz, 1H), 6.71 (s, 1H), 6.75 (s, 2H), 7.12-7.22 (m, 7H), 8.19 (br s, 1H).

EXAMPLE 2

2-(1-Methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(phenylmethyl)-1-piperazinyl]-2-h ydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0134] The title compound was prepared according to the method described in Example 1E, substituting benzyl bromide in place of 3,4-dioxomethylenebenzyl chloride.

[0135] MS: 721 (M+H)+.

[0136] 1H NMR (CDCl3) δ 0.68 (d, J=6.3 Hz, 3H), 0.84 (d, J=6.3 Hz, 3H), 1.36 (s, 9H), 1.40 (d, J=6.3, 6H), 2.04 (hextet, J=6.0 Hz, 1H), 2.28 (dt, J=9.3, 3.3 Hz, 1H), 2.45 (dt, J=12.0, 3.0 Hz, 1H), 2.58 (m, 2H), 2.75 (m, 2H), 2.88 (m, 2H), 3.33 (heptet, J=6.3 Hz, 1H), 3.37 (m, 1H), 3.46 (s, 2H), 3.79 (m, 1H), 3.85 (dd, J=8.4, 6.0 Hz, 1H), 4.22 (septet, J=4.5 Hz, 1H), 5.11 (dd, J=10.8, 7.5 Hz, 1H), 5.17 (d, J=3.3 Hz, 2H), 6.13 (d, J=9.0 Hz, 2H), 7.12-7.33 (m, 11 H), 8.02 (s, 1H), 8.26 (br s, 1H).

EXAMPLE 3

2-(1-Methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-[(4-fluorophenyl)methyl]-1-piperaz inyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0137] The title compound was prepared according to the method described in Example 1E, substituting 4-fluorobenzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

[0138] MS: 739 (M+H)+.

[0139] 1H NMR (CDCl3) δ 0.67 (d, J=6.3 Hz, 3H), 0.84 (d, J=6.3 Hz, 3H), 1.35 (s, 9H), 1.41 (d, J=7.5, 6H), 2.06 (hextet, J=6.0 Hz, 1H), 2.30 (m, 1H), 2.48 (m, 1H), 2.59 (m, 1H), 2.73 (m, 2H), 2.83 (m, 2H), 2.92 (m, 2H), 3.33 (AB, J=15.0 Hz, 2H), 3.37 (m, 1H), 3.44 (s, 2H), 3.83 (m, 2H), 4.22 (m, 1H), 5.09 (m, 1H), 5.17 (d, J=4.8 Hz, 2H), 6.19 (d, J=9.0 Hz, 1H), 7.03 (d, J=8.4 Hz, 1H), 7.12-7.22 (m, 9H), 8.09 (br s, 1H).

EXAMPLE 4

2-(1-Methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(5-thienylmethyl)-1-piperazinyl]- 2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0140] The title compound was prepared according to the method described in Example 1E, substituting 5-(chloromethyl)thiazole in place of 3,4-dioxomethylenebenzyl chloride.

[0141] MS: 728 (M+H)+.

[0142] 1H NMR (CDCl3) δ 0.68 (d, J=6.3 Hz, 3H), 0.83 (d, J=6.3 Hz, 3H), 1.38 (s, 9H), 1.41 (d, J=7.5, 6H), 2.06 (hextet, J=6.0 Hz, 1H), 2.33 (m, 1H), 2.58 (m, 2H), 2.73 (m, 2H), 2.83 (m, 2H), 2.90 (m, 1H), 3.33 (heptet, J=4.5 Hz, 1H), 3.36 (m, 1H), 3.73 (d, J=3.0 Hz, 2H), 3.80 (m, 1H), 3.83 (dd, J=7.5, 6.0 Hz, 1H), 4.22 (septet, J=4.5 Hz, 1H), 5.07 (d, J=7.5 Hz, 1H), 5.17 (d, J=4.5 Hz, 2H), 6.15 (d, J=7.5 Hz, 1H), 7.12-7.22 (m, 8H), 7.40 (m, 1H), 7.73 (s, 1H), 7.79 (s, 1H), 8.80 (s, 1H).

EXAMPLE 5

2-(1-Methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-[4-(3-hydroxyphenyl)methyl]-1-pip erazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0143] The title compound was prepared according to the method described in Example 1E, substituting 3-hydroxybenzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

[0144] MS: 7:37 (M+H)+.

[0145] 1H NMR (CDCl3) δ 0.67 (d, J=6.3 Hz, 3H), 0.83 (d, J=6.3 Hz, 3H), 0.96 (m, 1H), 1.37 (s, 9H), 1.40 (d, J=7.5, 6H), 2.03 (hextet, J=6.0 Hz, 1H), 2.32 (m, 1H), 2.45 (m, 1H), 2.61 (m, 2H), 2.76 (m, 2H), 2.85 (m, 2H,), 2.89 (m, 2H), 3.32 (m, 1H), 3.37 (s, 1H), 3.42 (m, 1H), 3.61 (t, J=8.4 Hz, 1H), 3.87 (m, 2H), 4.22 (m, 1H), 4.63 (s, 1H), 5.16 (s, 2H), 6.36 (m, 1H), 6.78 (d, J=7.5 Hz, 2H), 7.12-7.22 (m, 9H), 8.19 (br s, 1H).

EXAMPLE 6

2-(1-Methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(3-pyridinylmethyl)-1-piperazinyl ]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0146] The title compound was prepared according to the method described in Example 1E, substituting 3-picolyl chloride hydrochloride in place of 3,4-dioxomethylenebenzyl chloride.

[0147] MS: 722 (M+H)+.

[0148] 1H NMR (CDCl) δ 0.68 (d, J=6.3 Hz, 3H), 0.83 (d, J=6.3 Hz, 3H), 1.36 (s, 9H), 1.41 (d, J=7.5 Hz, 6H), 2.06 (Hextet, J=4.5 Hz, 1H), 2.47 (m, 1H), 2.60 (m, 2H), 2.71 (m, 2H), 2.81 (m, 1H), 2.83 (m, 2H), 2.92 (m, 1H), 3.34 (heptet, J=4.5 Hz, 1H), 3.35 (m, 1H), 3.51 (s, 2H), 3.81 (m, 1H), 3.85 (m, 1H), 4.22 (m, 1H), 4.75 (m, 1H), 5.07 (d, J=9.0 Hz, 1H), 5.17 (d, J=7.8 Hz, 2H), 6.15 (d, J=7.8 Hz, 1H), 7.12-7.22 (m, 8H), 7.61 (d, J=7.8 Hz, 1H), 7.89 (br s, 1H), 8.55 (d, J=3 Hz, 1H), 8.56 (d, J=6.0 Hz, 1H).

EXAMPLE 7

2-(1-Methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(4-pyridinylmethyl)-1-piperazinyl ]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0149] The title compound was prepared according to the method described in Example 1E substituting 4-picolyl chloride hydrochloride in place of 3,4-dioxomethylenebenzyl chloride.

[0150] MS: 722 (M+H)+.

[0151] 1H NMR (CDCl3) δ 0.65 (d, J=6.3 Hz, 3H), 0.94 (d, J=6.3 Hz, 3H), 1.37 (s, 9H), 1.41 (d, J=7.5, 6H), 2.07 (hex let, J=6.0 Hz, 1H), 2.37 (m, 1H), 2.57 (m, 1H), 2.62 (m, 2H), 2.69 (m, 2H), 2.81 (m, 2H), 2.91 (m, 1H), 3.32 (m, 1H), 3.34 (m, 1H), 3.49 (AB, J=15.0 Hz, 2H), 3.80 (m, 1H), 3.85 (m, 1H), 4.21 (m, 1H), 4.62 (m, 1H), 5.06 (d, J=7.5 Hz, 1H), 5.14 (m, 1H), 5.17 (d, J=4.5 Hz, 2H), 6.14 (d, J=8.4 Hz, 1H), 7.12-7.22 (m, 8H), 7.77 (br s, 1H), 8.08 (d, J=6.0 Hz, 2H).

EXAMPLE 8

2-(1-Methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-[(4-hydroxyphenyl)methyl]-1-piper azinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0152] The title compound was prepared according to the method described in Example 1E, substituting 4-hydroxybenzyl chloride hydrochloride in place of 3,4-dioxomethylenebenzyl chloride.

[0153] MS: 737 (M+H)+.

[0154] 1H NMR (CDCl3) δ 0.68 (d, J=6.3 Hz, 3H), 0.83 (d, J=6.3 Hz, 3H), 1.35 (s, 9H), 1.41 (ed, J=7.5, 6H), 2.03 (hextet, J=4.5 Hz, 1H), 2.27 (dt, J=7.5, 3.0 Hz, 1H), 2.36 (dd, J=12.0, 3.0 Hz, 1H), 2.59 (m, 2H), 2.74 (m, 2H), 2.87 (m, 2H), 2.79 (m, 1H), 2.92 (m, 1H), 3.32 (m, 1H), 3.33 (AB, J=15.0 Hz, 2H), 3.40 (m, 2H), 3.72 (m, 1H), 3.78 (dd, J=8.4, 6.0 Hz, 1H), 4.22 (septet, J=4.5 Hz, 1H), 5.22 (d, J=4.5 Hz, 2H), 5.23 (m, 1H), 6.17 (m, 1H), 6.40 (d, J=9.0 Hz, 1H), 6.80 (d, J=8.7 Hz, 2H), 7.09-7.21 (m, 8H), 8.31 (br s, 1H).

EXAMPLE 9

2-(1-Methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1H-benzimidiazol-2-ylmethyl)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-pi perazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0155] The title compound was prepared according to the method described in Example 1E, substituting 2-(chloromethyl)benzimidazole in place of 3,4-dioxomethylenebenzyl chloride.

[0156] MS: 761 (M+H)+.

[0157] 1H NMR (CDCl3) δ 0.62 (d, J=6.3 Hz, 3H), 0.83 (d, J=6.3 Hz, 3H), 1.41 (d, J=7.5, 6H), 1.43 (s, 9H), 2.11 (hextet, J=4.5 Hz, 1H), 2.52 (m, 1H), 2.57 (dd, J=12.0, 3.0 Hz, 1H), 2.72 (m, 2H), 2.79 (m, 3H), 3.02 (dd, J=15.0, 4.5 Hz, 1H), 3.17 (m, 3H), 3.34 (heptet, J=4.5 Hz, 1H), 3.85 (m, 2H), 3.95 (d, J=9.0 Hz, 1H), 4.25 (m, 1H), 4.98 (m, 1H), 5.13 (s, 2H), 5.17 (m, 1H), 6.17 (m, 1H), 7.12-7.28 (m, 11 H), 7.63 (m, 2H).

EXAMPLE 10

2-(1-Methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(2-quinolinylmethyl)-1-piperaziny l]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0158] The title compound was prepared according to the method described in Example 1E, substituting 2-(chloromethyl)quinoline monohydrochloride in place of 3,4-dioxomethylenebenzyl chloride.

[0159] MS: 772 (M+H)+.

[0160] 1H NMR (CDCl3) δ 0.67 (d, J=6.3 Hz, 3H), 0.83 (d, J=6.3 Hz, 3H), 1.37 (s, 9H), 1.40 (d, J=7.5, 6H), 2.05 (hextet, J=6.0 Hz, 1H), 2.50 (m, 1H), 2.61 (m, 2H), 2.70 (m, 1H), 2.71 (m, 2H), 2.85 (m, 2H), 2.92 (m, 1H), 3.32 (m, 1H), 3.47 (m, 1H), 3.82 (m, 2H), 3.85 (m, 1H), 4.22 (septet, J=4.5 Hz, 1H), 4.79 (s, 1H), 5.10 (m, 1H), 5.17 (d, J=8.4 Hz, 2H), 6.11 (s, 1H), 7.12-7.22 (m, 9H), 7.47 (d, J=6.6 Hz, 1H), 7.56 (t, J=7.5 Hz, 1H), 7.73 (t, J=8.4 Hz, 1H), 7.83 (d, J=8.4 Hz, 1H), 8.07 (d, J=7.5 Hz, 1H), 8.17 (d, J=8.4 Hz, 1H).

EXAMPLE 11

2-(1-Methylethyl)-4-thiazolylmethyl[(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-p iperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0161] The title compound was prepared according to the method described in Example 1E, substituting 3,4-dimethoxy benzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

[0162] MS: 781 (M+H)+.

[0163] 1H NMR (CDCl3) δ 0.68 (d, J=6.3 Hz, 3H), 0.84 (d, J=6.3 Hz, 3H), 1.35 (s, 9H), 1.41 (d, J=6.3, 6H), 2.05 (hextet, J=6.0 Hz, 1H), 2.25 (m, 1H), 2.45 (m, 1H), 2.58 (m, 2H), 2.74 (m, 3H), 2.84 (m, 2H), 2.90 (m, 1H), 3.35 (heptet, J=6.3 Hz, 1H), 3.40 (AB, J=15.0 Hz, 2H), 3.81 (m, 1H), 3.87 (s, 3H), 3.88 (s, 3H), 4.21 (m, 1H), 5.08 (m, 1H), 5.17 (d, J=3.3 Hz, 2H), 6.12 (m, 1H), 6.26 (s, 1H), 6.82 (s, 2H), 7.10-7.22 (m, 8H), 8.21 (br s, 1H).

EXAMPLE 12

N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propy l]amino]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea

[0164] 12A. N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-(1,1-dimethylethyloxy(carbonyl))-1-piperazinyl]-2-hydroxy-1-(phenylme thyl)propyl]amino]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea.

[0165] A solution of 1.5 g (3.3 mmol) of 1-[(1S,2R)-3-[(2S)-4-(1,1-dimethylethyloxy(carbonyl))-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl ]amine in 7 ml of THF and 7 ml of methylene chloride was prepared and treated with 1.0 g (3.3 mmol) of N-methyl-N-[(((2-isopropyl-4-thiazolyl)methyl)amino)carbonyl]-L-valine, 642 mg (3.3 mmol) of EDAC, 45 mg (0.33 mmol) of HOBT, and 466 μl (3.3 mmol) of triethyl amine. The solution was stirred at room temperature for 6 hours, and concentrated in vacuo. The residue was taken up with ethyl acetate, washed with equal portions (15 ml) of 3 N HCl solution, 10% aqueous NaHCO3, and saturated brine. The organic layer was dried over MgSO4 and concentrated in vacuo. The mixture was purified by chromatography on silica gel using 5% methanol:methylene chloride to provide the title compound (yield: 2.4 g; 98%).

[0166] 12B. N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methy lpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea.

[0167] A solution of 2.4 g (3.2 mmol) of N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(1,1-dimethylethyloxy(carbonyl))-1-piperazinyl]-2-hydroxy-1-(phenylme thyl)propyl]amino]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea in 20 ml of dichloromethane, was prepared and treated with 2 ml of trifluoroacetic acid. The reaction was monitored by TLC. After the reaction was completed, the solvent was removed at reduced pressure. The residue was dissolved in methylene chloride, washed with saturated aqueous NaHCO3, and saturated brine. The organic layer was dried over Na2SO4 and concentrated in vacuo to provide the title compound as a light oil (yield: 2.0 g; 90%). The product was used in Example 12C without further purification.

[0168] 12C. N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]a mino]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea.

[0169] The title compound was prepared according to the method described in Example 1E, starting with the product, prepared in Example 12B, and substituting 5-thiazolylmethyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

[0170] MS: 741 (M+H)+.

[0171] 1H NMR (CDCl3) δ 0.75 (d, J=6.3 Hz, 3H), 0.89 (d, J=6.3 Hz, 3H), 1.33 (d, J=3.0 Hz, 3H), 1.45 (d, J=3.0 Hz, 3H), 1.47 (s, 9H), 2.18 (m, 1H), 2.40 (dt, J=7.5, 3.0 Hz, 1H), 2.55 (m, 1H), 2.62 (m, 4H), 2.73 (dd, J=15.0, 4.5 Hz, 2H), 2.89 (m, 1H), 2.95 (s, 3H), 3.22 (heptet, J=6.0 Hz, 1H), 3.27 (d, J=3.0 Hz, 2H), 3.73 (d, J=3.0 Hz, 2H), 3.87 (m, 1H), 3.99 (dd, J=6.3, 6.0 Hz, 1H), 4.16 (septet, J=4.5 Hz, 1H), 4.37 (s, 2H), 4.61 (br s, 1H), 6.00 (br s, 1H), 6.39 (d, J=9.0 Hz, 1H), 6.97 (s, 1H), 7.08-7.17 (m, 5H), 7.54 (s, 1H), 7.72 (s, 1H), 8.79 (s, 1H).

EXAMPLE 13

N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-(phenylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]ami no]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea

[0172] The title compound was prepared according to the method described in Example 12C, substituting benzyl bromide in place of 5-thiazolylmethyl chloride.

[0173] MS: 734 (M+H)+.

[0174] 1H NMR (CDCl3) δ 0.75 (d, J=6.3 Hz, 3H), 0.88 (d, J=6.3 Hz, 3H), 1.45 (d, J=7.5 Hz, 6H), 1.46 (s, 9H), 2.15 (hextet, J=6.3 Hz, 1H), 2.32 (dt, J=9.0, 3.0 Hz, 1H), 2.51 (dd, J=8.4, 3.0 Hz, 1H), 2.59 (dd, J=8.4, 3.0 Hz, 1H), 2.70 (m, 2H), 2.78 (m, 2H), 2.95 (s, 3H), 3.24 (heptet, J=7.5 Hz, 1H), 3.32 (t, J=3.0 Hz, 1H), 3.45 (s, 2H), 3.81 (m, 1H), 4.01 (dd, J=7.5, 6.0 Hz, 1H), 4.17 (septet, J=4.5 Hz, 1H), 4.48 (s, 2H), 4.84 (br s, 1H), 5.89 (m, 1H), 6.48 (d, J=9.0 Hz, 1H), 6.95 (s, 1H), 7.07-7.18 (m, 5H), 7.25-7.34 (m, 5H), 8.04 (br s, 1H).

EXAMPLE 14

N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-[(4-hydroxy-3-methoxyphenyl)methyl]-1-piperazinyl]-2-hydroxy-1-(ph enylmethyl)propyl]amino]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea

[0175] The title compound was prepared according to the method described in Example 12C substituting 3-methoxy-4-hydroxybenzyl chloride in place of 5-thiazolylmethyl chloride.

[0176] MS: 780 (M+H)+.

[0177] 1H NMR (CDCl3) δ 0.76 (d, J=6.3 Hz, 3H), 0.88 (d, J=6.3 Hz, 3H), 1.35 (s, 9H), 1.36 (d, J=7.5, 6H), 2.17 (hextet, J=6.0 Hz, 1H), 2.31 (dt, J=7.5, 3.0 Hz, 1H), 2.53 (m, 2H), 2.63 (m, 2H), 2.78 (m, 2H), 2.91 (m, 1H), 2.96 (s, 3H), 3.26 (q, J=7.5 Hz, 2H), 3.29 (m, 1H), 3.38 (AB, J=15.0 Hz, 2H), 3.83 (m, 1H), 3.89 (s, 3H), 4.01 (dd, J=7.5, 6.0 Hz, 1H), 4.17 (septet, J=4.5 Hz, 1H), 4.38 (s, 2H), 4.77 (m, 1H), 5.58 (s, 1H), 5.92 (m, 1H), 6.38 (d, J=9.0 Hz, 1H), 6.77 (m, 2H), 6.87 (d, J=8.4 Hz, 1H), 6.96 (s, 1H), 7.12-7.22 (m, 6H), 7.96 (br s, 1H).

EXAMPLE 15

2-Methyl-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,3-benzodioxol-5-ylmethyl)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl ]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0178] 15A. 2-Methyl-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,1-dimethylethyloxy(carbonyl))-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperaziny l]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0179] The title compound was prepared according to the method of Example 1C substituting [(2-methyl-4-thiazolyl)methyloxycarbonyl]-L-valine in place of [(2-isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine. This was followed by treatment of the product with trifluoroacetic acid according to the method of Example 1D

[0180] 15B. 2-Methyl-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,3-benzodioxol-5-ylmethyl)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2 -hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0181] A solution of 98 mg (0.16 mmol) of 2-methyl-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,1-dimethylethyloxy(carbonyl))-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperaziny l]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate in 1 ml of DMF was treated with 30 mg (0.18 mmol)l of 3,4-dioxomethylenebenzyl chloride followed by 43 μl (0.24 mmol) of N,N-diisopropyl ethyl amine. The solution was stirred at room temperature over night. After stirring, the solution was partitioned between ethyl acetate and saturated sodium bicarbonate solution. The organic solution was washed with brine, dried over sodium sulfate, and concentrated in vacuo. The mixture was purified by chromatography on silica gel, using 5% methanol:methylene chloride, to provide the title compound (yield: 31 mg; 26%).

[0182] MS: 737 (M+H)+.

[0183] 1H NMR (CDCl3) δ 0.67 (d, J=6.3 Hz, 3H), 0.84 (d, J=6.3 Hz, 3H), 1.37 (s, 9H), 2.05 (hextet, J=4.5 Hz, 1H), 2.26 (m, 1H), 2.41 (dd, J=12.0, 3.0 Hz, 1H), 2.55 (m, 1H), 2.59 (dd, J=9.3, 3.0 Hz, 2H), 2.72 (m, 2H), 2.73 (s, 3H), 2.76 (m, 1H), 2.83 (m, 2H), 2.91 (m, 1H), 3.35 (m, 1H), 3.47 (s, 2H), 3.79 (m, 1H), 3.86 (dd, J=7.5, 6.0 Hz, 1H), 4.22 (m, 1H), 4.93 (m, 1H), 5.62 (m, 2H), 5.65 (d, J=3.0 Hz, 2H), 5.96 (s, 2H), 6.18 (d, J=9.0 Hz, 1H), 6.70 (d, J=7.5 Hz, 1H), 6.75 (s, 1H), 6.78 (d, J=7.5 Hz, 1H), 7.12-7.22 (m, 6H), 8.19 (br s, 1H).

EXAMPLE 16

2-Methyl-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazin yl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0184] The title compound was prepared according to the method described in Example 15B, substituting 3,4-dimethoxybenzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

[0185] MS: 753 (M+H)+.

[0186] 1H NMR (DMSO-d6) δ 0.67 (d, J=6.3 Hz, 3H), 0.69 (d, J=6.3 Hz, 3H), 1.23 (s, 9H), 1.78 (hextet, J=4.5 Hz, 1H), 2.22 (m, 2H), 2.27 (m, 1H), 2.32 (m, 1H), 2.53 (m, 3H), 2.63 (s, 3H), 2.67 (m, 1H), 2.87 (m, 2H), 2.98 (m, 1H), 3.33 (m, 3H), 3.63 (m, 1H), 3.72 (s, 3H), 3.73 (s, 3H), 4.05 (m, 1H), 4.86 (d, J=9.0 Hz, 1H), 5.00 (s, 2H), 6.79 (d, J=7.5 Hz, 1H), 6.87 (s, 1H), 6.89 (d, J=7.5 Hz, 1H), 7.10 (d, J=6.0 Hz, 2H), 7.18 (d, J=6.3 Hz, 2H), 7.26 (d, J=7.5 Hz, 2H), 7.41 (s, 1H), 7.53 (s, 1H), 7.67 (d, J=9.0 Hz, 1H).

EXAMPLE 17

2-Methyl-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-[(4-fluorophenyl)methyl]-1-piperazinyl]-2- hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0187] The title compound was prepared according to the method described in Example 15B, substituting 4-fluorobenzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

[0188] MS: 711 (M+H)+.

[0189] 1H NMR (CDCl3) δ 0.68 (d, J=6.3 Hz, 3H), 0.84 (d, J=6.3 Hz, 3H), 1.35 (s, 9H), 2.06 (hextet, J=6.0 Hz, 1H), 2.28 (m, 1H), 2.47 (m, 1H), 2.55 (m, 1H), 2.59 (m, 2H), 2.71 (m, 2H), 2.73 (s, 3H), 2.76 (m, 1H), 2.83 (m, 2H), 2.90 (m, 1H), 3.35 (m, 1H), 3.43 (s, 2H), 3.80 (m, 1H), 3.86 (dd, J=7.5, 6.0 Hz, 1H), 4.22 (septet, J=4.5 Hz, 1H), 5.10 (m, 1H), 5.14 (s, 2H), 6.15 (d, J=7.5 Hz, 1H), 7.03 (t, J=8.4 Hz, 2H), 7.12-7.22 (m, 8H), 8.21 (br s, 1H).

EXAMPLE 18

2-Ethyl-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl ]-2-hydroxy1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0190] 18A. 2-Ethyl-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-[1,1-dimethylethyloxy(carbonyl)]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl] -2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0191] The title compound was prepared according to the method of Example 1C, substituting [(2-ethyl-4-thiazolyl)methyloxycarbonyl]-L-valine in place of [(2-isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine. This was followed by treatment of the product with trifluoroacetic acid according to the method of Example 1D.

[0192] 18B. 2-Ethyl-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2 -hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0193] The title compound was prepared by reacting 2-ethyl-4-thiazolylmethyl(1S)-1-[[[(1S,2R)-3-[(2S)-4-[1,1-dimethylethyl-oxy(carbonyl)]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl] -2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate, 98 mg (0.16 mmol), with 3,4-dimethoxybenzyl chloride, 30 mg (0.18 mmol), according to the method described in Example 15(B).

[0194] MS: 767 (M+H)+.

[0195] 1H NMR (CDCl3) δ 0.67 (d, J=6.3 Hz, 3H), 0.84 (d, J=6.3 Hz, 3H), 1.36 (s, 9H), 1.40 (d, J=7.5, 6H), 2.05 (hextet, J=6.0 Hz, 1H), 2.23 (m, 1H), 2.48 (m, 1H), 2.58 (dd, J=12.0, 3.0 Hz, 2H), 2.71 (m, 2H), 2.83 (m, 1H), 2.90 (m, 1H), 3.05 (q, J=7.5 Hz, 2H), 3.31 (m, 1H), 3.35 (m, 1H), 3.40 (AB, J=15.0 Hz, 2H), 3.88 (s, 3H), 3.89 (s, 3H), 4.22 (m, 1H), 4.88 (m, 1H), 5.08 (m, 1H), 5.16 (s, 2H), 6.11 (d, J=7.5 Hz, 1H), 6.77 (s, 1H), 6.82 (s, 2H), 7.12-7.22 (m, 8H), 8.21 (br s, 1H).

EXAMPLE 19

2-(1-Methylethyl)-5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1- piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0196] 19A. 2-(1-Methylethyl)-5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-[1,1-dimethylethyloxy(carbonyl)]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-p iperazinyl[-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0197] A solution of 1.5 g (3.3 mmol) of 1-[(1S,2R)-3-[(2S)-4-(1,1-dimethylethyloxy(carbonyl))-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl ]amine in 7 ml of THF and 7 ml of methylene chloride was prepared and treated with 1.0 g (3.3 mmol) of [(2-isopropyl-5-thiazolyl)methyloxycarbonyl]-L-valine, 642 mg (3.3 mmol) of EDAC, 45 mg (0.33 mmol) of HOBT, and 466 μl (3.3 mmol) of triethyl amine. The solution was stirred at room temperature for 6 hours, and concentrated in vacuo. The residue was taken up with ethyl acetate, washed with equal portions (15 mL) of 3 N HCl solution, 10% aqueous NaHCO3, and saturated brine, dried over MgSO4, and concentrated in vacuo. The resulting mixture was purified by chromatography on silica gel, using 5% methanol:methylene chloride, to provide the title compound yield: 2.1 g, 88%).

[0198] MS: 731 (M+H)+.

[0199] 1H NMR (DMSO-d6) δ 1.08 (d, J=7 Hz, 6H), 2.78 (heptet, J=7 Hz, 1H), 9.06 (br s, 1H), 9.30 (br s, 1H).

[0200] 19B. 2-(1-Methylethyl)-5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-pip erazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0201] The title compound was prepared in two steps. First, 2-(1-methylethyl)-5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-[1,1-dimethylethyloxy(carbonyl)]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-p iperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate was treated with trifluoroacetic acid according to the method described in Example 1D. This was followed by akylation of the product according to the method of Example 1E, substituting 3,4-dimethylbenzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

[0202] MS: 781 (M+H)+.

[0203] 1H NMR (CDCl3) δ 0.72 (d, J=6.3 Hz, 3H), 0.86 (d, J=6.3 Hz, 3H), 1.37 (s, 9H), 1.40 (d, J=6.3, 6H), 2.01 (hextet, J=6.0 Hz, 1H), 2.22 (dt, J=7.5, 3.0 Hz, 1H), 2.43 (m, 1H), 2.55 (m, 2H), 2.73 (m, 3H), 2.88 (m, 2H), 2.93 (m, 1H), 3.30 (heptet, J=6.3 Hz, 1H), 3.38 (m, 1H), 3.41 (AB, J=15.0 Hz, 2H), 3.80 (m, 1H), 3.87 (s, 3H), 3.88 (s, 3H), 4.22 (septet, J=4.5 Hz, 1H), 5.07 (d, J=8.4 Hz, 1H), 5.20 (d, J=3.0 Hz, 2H), 6.06 (d, J=9.0 Hz, 1H), 6.76 (s, 1H), 6.82 (s, 2H), 7.12-7.22 (m, 7H), 7.61 (s, 1H), 8.28 (br s, 1H).

EXAMPLE 20

2-Ethyl-5-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl ]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0204] 20A. 2-Ethyl-5-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-[1,1-dimethylethyloxy(carbonyl)]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl] -2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0205] The title compound was prepared according to the method described in Example 19A, substituting [(2-ethyl-5-thiazolyl)methyloxycarbonyl]-L-valine in place of [(2-isopropyl-5-thiazolyl)methyloxycarbonyl]-L-valine.

[0206] 20B. 2-Ethyl-5-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2 -hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0207] The title compound was prepared in two steps. First, 2-ethyl-5-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-[1,1-dimethylethyloxy(carbonyl)]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl] -2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate was treated with trifluoroacetic acid according to the method described in Example 1D. This was followed by akylation of the product according to the method of Example 1E, substituting 3,4-dimethylbenzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

[0208] MS: 767 (M+H)+.

[0209] 1H NMR (CDCl3) δ 0.73 (d, J 6.3 Hz, 3H), 0.86 (d, J=6.3 Hz, 3H), 1.37 (s, 9H), 1.38 (t, J=7.5, 3H), 2.02 (m, 1H), 2.23 (dt, J=7.5, 3.0 Hz, 1H), 2.45 (m, 1H), 2.56 (m, 2H), 2.72 (m, 2H), 2.77 (m, 1H), 2.89 (m, 2H), 3.02 (q, J=7.5 Hz, 2H), 3.39 (t, J=3.3 Hz, 1H), 3.41 (AB, J=15.0 Hz, 2H), 3.81 (m, 2H), 3.88 (s, 3H), 3.89 (s, 3H), 4.21 (m, 1H), 5.06 (d, J=9.0 Hz, 1H), 5.20 (d, J=5.4 Hz, 2H), 6.06 (d, J=9.0 Hz, 1H), 6.76 (s, 1H), 6.82 (s, 2H), 7.13-7.23 (m, 7H), 7.61 (s, 1H), 8.27 (br s, 1H).

EXAMPLE 21

2-Methyl-5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazin yl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0210] 21 A. 2-Methyl-5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-[1,1-dimethylethyloxy(carbonyl)]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperaziny l]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0211] The title compound was prepared according to the method described in Example 19A, substituting [(2-methyl-5-thiazolyl)methyloxycarbonyl]-L-valine in place of [(2-isopropyl-5-thiazolyl)methyloxycarbonyl]-L-valine.

[0212] 21B. 2-Methyl-5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-[(3,4-dimethoxylphenyl)methyl]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl] -2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0213] The title compound was prepared in two steps. First, 2-methyl-5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-4-[1,1-dimethylethyloxy(carbonyl)]-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperaziny l]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate was treated with trifluoroacetic acid according to the method described in Example 1D. This was followed by akylation of the product according to the method of Example 1E, substituting 3,4-dimethylbenzyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

[0214] MS: 753 (M+H)+.

[0215] 1H NMR (CDCl3) δ 0.72 (d, J=6.3 Hz, 3H), 0.86 (d, J=6.3 Hz, 3H), 1.46 (s, 9H), 2.01 (hextet, J=6.0 Hz, 1H), 2.22 (dt, J=7.5, 3.0 Hz, 1H), 2.43 (dd, J=12.0, 3.0 Hz, 1H), 2.56 (m, 2H), 2.69 (s, 3H), 2.72 (m, 1H), 2.77 (m, 1H), 2.86 (m, 2H), 2.92 (m, 1H), 3.39 (t, J=3.0 Hz, 1H), 3.41 (AB, J=15.0 Hz, 2H), 3.80 (m, 2H), 3.87 (s, 3H), 3.88 (s, 3H), 4.21 (septet, J=4.5 Hz, 1H), 5.06 (d, J=9.0 Hz, 1H), 5.19 (AB, J=9.6 Hz, 2H), 6.07 (d, J=9.0 Hz, 1H), 6.75 (s, 1H), 6.82 (s, 2H), 7.12-7.22 (m, 7H), 7.58 (s, 1H), 8.27 (br s, 1H).

EXAMPLE 22

N′-[(1R)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propy l]amino]carbonyl]-2-methylpropyl]-N-methyl-N-(5-thiazolylmethyl)urea

[0216] 22A. N′-[(1R)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-[1,1-dimethylethyloxy(carbonyl)]-1-piperazinyl]-2-hydroxy-1-(phenylme thyl)propyl]amino]carbonyl]-2-methylpropyl]-N-methyl-N-(5-thiazolylmethyl)urea.

[0217] The title compound was prepared according to the method described in Example 19A, substituting [N-methyl-(5-thiazolyl)methylaminocarbonyl]-D-valine in place of [(2-isopropyl-5-thiazolyl)methyloxycarbonyl]-L-valine.

[0218] 22B. N′-[(1R)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]a mino]carbonyl]-2-methylpropyl]-N-methyl-N-(5-thiazolylmethyl)urea.

[0219] The title compound was prepared in two steps. N′-[(1R)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-[1,1-dimethylethyloxy(carbonyl)]-1-piperazinyl]-2-hydroxy-1-(phenylme thyl)propyl]amino]carbonyl]-2-methylpropyl]-N-methyl-N-(5-thiazolylmethyl)urea was treated with trifluoroacetic acid according to the method described in Example 1D. This was followed by akylation of the product according to the method of Example 1E, substituting 5-thiazolyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

[0220] MS: 699 (M+H)+.

[0221] 1H NMR CDCl3) δ 0.58 (d, J=6.3 Hz, 3H), 0.66 (d, J=6.3 Hz, 3H), 1.38 (s, 9H), 1.75 (m, 1H), 2.35 (m, 1H), 2.60 (m, 2H), 2.70 (m, 2H), 2.85 (m, 2H), 2.86 (s, 3H), 2.92 (m, 3H), 3.30 (t, J=3.3 Hz, 1H), 3.74 (s, 2H), 3.88 (m, 1H), 3.97 (dd, J=8.4, 6.0 Hz, 1H), 4.28 (m, 2H), 4.53 (br s, 1H), 4.65 (d, J=3.3 Hz, 2H), 5.03 (d, J=7.5, 1 H), 6.09 (d, J=9.0 Hz, 1H), 7.17-7.27 (m, 5H), 7.73 (s, 2H), 8.73 (s, 1H), 8.80 (s, 1H).

EXAMPLE 23

N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propy l]amino]carbonyl]-2-methylpropyl]-N-methyl-N-(5-thiazolylmethyl)urea

[0222] 23A. N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-[1,1-dimethylethyloxy(carbonyl)]-1-piperazinyl]-2-hydroxy-1-(phenylme thyl)propyl]amino]carbonyl]-2-methylpropyl]-N-methyl-N-(5-thiazolylmethyl)urea.

[0223] The title compound was prepared according to the method described in Example 19A, substituting [N-methyl-(5-thiazolyl)methylaminocarbonyl]-L-valine in place of [(2-isopropyl-5-thiazolyl)methyloxycarbonyl]-L-valine.

[0224] 23B. N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]a mino]carbonyl]-2-methylpropyl]-N-methyl-N-(5-thiazolylmethyl)urea.

[0225] The title compound was prepared in two steps. First, N′-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-[1,1-dimethylethyloxy(carbonyl)]-1-piperazinyl]-2-hydroxy-1-(phenylme thyl)propyl]amino]carbonyl]-2-methylpropyl]-N-methyl-N-(5-thiazolylmethyl)urea was treated with trifluoroacetic acid according to the method described in Example 1D. This was followed by akylation of the product according to the method of Example 1E, substituting 5-thiazolyl chloride in place of 3,4-dioxomethylenebenzyl chloride.

[0226] MS: 699 (M+H)+.

[0227] 1H NMR (CDCl3) δ 0.78 (d, J=6.3 Hz, 3H), 0.87 (d, J=6.3 Hz, 3H), 1.49 (s, 9H), 2.02 (m, 1H), 2.33 (m, 2H), 2.57 (m, 2H), 2.75 (m, 2H), 2.82 (s, 3H), 2.89 (m, 3H), 3.38 (t, J=3.0 Hz, 1H), 3.73 (d, J=3.3 Hz, 2H), 3.79 (m, 1H), 4.03 (dd, J=8.4, 6.3 Hz, 1H), 4.20 (m, 1H), 4.66 (AB, J=15 Hz, 2H), 4.78 (m, 1H), 4.81 (d, J=8.4 Hz, 2H), 6.11 (d, J=9.0 Hz, 1H), 7.11-7.23 (m, 5H), 7.73 (s, 1H), 7.76 (s, 1H), 7.82 (br s, 1H), 8.75 (s, 1H), 8.80 (s, 1H).

EXAMPLE 24

5-Thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(phenylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylme thyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0228] 24A. 5-Thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-[1,1-dimethylethyloxy(carbonyl)]-1-piperazinyl]-2-hydr oxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0229] The title compound was prepared according to the method described in Example 19A, substituting [(5-thiazolyl)methyloxycarbonyl]-L-valine, in place of [(2-isopropyl-5-thiazolyl)methyloxycarbonyl]-L-valine.

[0230] 24B. 5-Thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(phenylmethyl)-1-piperazinyl]-2-hydroxy-1-(phenylmethy l)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0231] The title compound was prepared in two steps. First, 5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-[1,1-dimethylethyloxy(carbonyl)]-1-piperazinyl]-2-hydr oxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate was treated with trifluoroacetic acid according to the method described in Example 1D. This was followed by akylation of the product according to the method of Example 1E, substituting benzyl bromide in place of 3,4-dioxomethylenebenzyl chloride.

[0232] MS: 699 (M+H)+.

[0233] 1H NMR CDCl3) δ 0.78 (d, J=6.3 Hz, 3H), 0.87 (d, J=6.3 Hz, 3H), 1.49 (s, 9H), 2.02 (m, 1H), 2.33 (m, 2H), 2.57 (m, 2H), 2.75 (m, 2H), 2.82 (s, 3H), 2.89 (m, 3H), 3.38 (t, J=3.0 Hz, 1H), 3.73 (d, J=3.3 Hz, 2H), 3.79 (m, 1H), 4.03 (dd, J=8.4, 6.3 Hz, 1H), 4.20 (m, 1H), 4.66 (AB, J=15 Hz, 2H), 4.78 (m, 1H), 4.81 (d, J=8.4 Hz, 2H), 6.11 (d, J=9.0 Hz, 1H), 7.11-7.23 (m, 5H), 7.73 (s, 1H), 7.76 (s, 1H), 7.82 (br s, 1H), 8.75 (s, 1H), 8.80 (s, 1H).

EXAMPLE 25

5-Thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethyl)-1-piperazinyl]-2-hydroxy-1-(phen ylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0234] The title compound was prepared according to the method described in Example 24B, by akylation of 5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]ca rbonyl]-2-methylpropyl]carbamate with 5-thiazolyl methyl chloride in place of benzyl bromide.

[0235] MS: 686 (M+H)+.

[0236] 1H NMR (CDCl3) δ 0.62 (d, J=6.3 Hz, 3H), 0.86 (d, J=6.3 Hz, 3H), 1.39 (s, 9H), 2.01 (m, 1H), 2.30 (m, 1H), 2.52 (m, 1H), 2.67 (m, 2H), 2.71 (m, 1H), 2.75 (m, 2H), 2.87 (m, 2H), 2.92 (m, 1H), 3.29 (t, J=3.0 Hz, 1H), 3.74 (d, J=4.5 Hz, 2H), 4.80 (m, 2H), 4.85 (m, 1H), 4.21 (m, 1H), 5.08 (t, J=9.0 Hz, 1H), 5.18 (d, J=4.5 Hz, 1H), 6.07 (d, J=9.6 Hz, 2H), 7.11-7.24 (m, 5H), 7.74 (s, 1H), 7.88 (s, 1H), 8.80 (s, 1H), 8.81 (s, 1H).

EXAMPLE 26

N′-[(1S)-1-[[[(1S,2R)-3-[[[(1,1-Dimethylethyl)amino]carbonyl](2-methylethyl)propylamino]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl] -2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea

[0237] The title compound was prepared according to the method described in Example 12A, by coupling N-methyl-N-[(((2-isopropyl-4-thiazolyl)methyl)amino)-carbonyl]-L-valine with N-(3S-amino-2R-hydroxy-4-phenylbutyl)-N-(2-methylpropyl)-N′-(1,1-dimethylethyl)urea, prepared according to the method described in J. Med. Chem., 1993, 36, 288-291.

[0238] Elemental analysis: Theory: C: 62.34, H: 8.44, N: 13.64 Found: C: 59.60, H: 7.37, N: 12.86

Example 27

2-(1-Methylethyl)-4-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[[[(1,1-dimethylethyl)amino]carbonyl](2-methylethyl)propylamino]-2-hydroxy-1-(ph enylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0239] The title compound was prepared according to the method described in Example 26, by coupling N-[(2-isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine with N-(3S-amino-2R-hydroxy-4-phenylbutyl)-N-(2-methylpropyl)-N′-(1,1-dimethylethyl)urea, prepared according to the method described in J. Med. Chem., 1993, 36, 288-291.

[0240] Elemental analysis: Theory: C: 61.69, H: 8.13, N: 11.61 Found: C: 60.80, H: 8.21, N: 11.18

EXAMPLE 28

2-(1-Methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[[(4-aminophenyl)sulfonyl](1-methylethyl)amino]-2-hydroxy-1-(phenylmethyl)propyl] amino]carbonyl]-2-methylpropyl]carbamate

[0241] The title compound was prepared in two steps. First, N-isobutyl-2(R)-hydroxy-3(S)-amino-4-phenyl-(4′-nitrophenyl)sulfonamide, prepared according to the method described in J. Am. Chem. Soc., 1995, 117, 1181-1182, was coupled with [(2-isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine according to the method described in Example 1C. This was followed by hydrogenation of the product over Pd/C under 1 atmosphere of hydrogen. The catalyst was removed via filtration over a celite pad and the solvent was evaporated at reduced pressure to provide the title compound as a white foam.

[0242] MS: 674 (M+H)+.

[0243] 1H NMR (DMSO-d6) δ 0.65 (d, J=6.3 Hz, 3H), 0.67 (d, J=6.3 Hz, 3H), 0.77 (d, J=6.0 HZ, 3H), 0.81 (t, J=6.0 HZ, 3H), 1.32 (d, J=6.3 Hz, 6H), 1.77 (m, 1H), 1.90 (m, 1H), 2.60 (m, 2H), 2.65 (m, 1H), 2.72 (m, 1H), 2.83 (d, J=7.5 Hz, 1H), 2.89 (d, J=9.0 Hz, 1H), 3.00 (m, 2H), 3.25 (m, 1H), 3.61 (m, 1H), 3.75 (dd, J=9.0, 6.3 Hz, 1H), 3.94 (m, 1H), 4.96 (d, J=6.3 Hz, 1H), 5.02 (s, 2H), 5.98 (s, 2H), 6.60 (t, J=9.0 Hz, 2H), 7.10 (m, 2H), 7.15-7.22 (m, 3H), 7.39 (d, J=8.4 Hz, 2H), 7.43 (s, 1H), 7.72 (d, J=9.0 Hz, 1H).

EXAMPLE 29

N′-[(1S)-1-[[[(1S,2R)-3-[[(4-Aminophenyl)sulfonyl](1-methylethyl)amino]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]- N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea

[0244] The title compound was prepared in two steps. First, N-isopropyl-2(R)-hydroxy-3(S)-amino-4-phenyl-(4′-nitrophenyl)sulfonamide was coupled with N-methyl-N-[(((2-isopropyl-4-thiazolyl)methyl)amino)carbonyl]-L-valine according to the method described in Example 12A. This was followed by hydrogenation of the product over Pd/C under 1 atmosphere of hydrogen. The catalyst was removed via filtration over a celite pad and the solvent was evaporated at reduced pressure to provide the title compound as a white foam.

[0245] MS: 674 (M+H)+.

[0246] Elemental analysis: Theory: C: 58.93, H: 7.14, N: 12.50 Found: C: 58.30, H: 7.28, N: 12.11

EXAMPLE 30

2-(1-Methylethyl)-5-thiazolylmethyl-[(1S)-1-[[[(1S,2R)-3-[(3S)-3-[[(1,1-dimethylethyl)amino]carbonyl](4aα,8aα)octahydro-2-isoquinolinyl]- 2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate

[0247] The title compound was prepared according to the method described in Example 1C, by coupling 2-(3(S)-amino-2(R)-hydroxy-4-phenylbutyl)-N-tert-butyldecahydro-(4aS,8aS)-isoquinoline-3(S)-carboxamide with [(2-isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine, prepared according to the method described in J. Org Chem., 1994, 59, 3656-3664.

[0248] Elemental analysis: Theory: C: 65.01, H: 8.35, N: 10.25 Found: C: 63.70, H: 8.19, N: 10.05

EXAMPLE 31

N′-[(1S)-1-[[[(1S,2 R)-3-[(3S)-3-[[(1,1-Dimethylethyl)amino]carbonyl](4aα,8aα)-octahydro-2-isoquinolinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-met hylpropyl]-N-methyl-N-[2-(1-methylethyl)-5-thiazolylmethyl]urea

[0249] The title compound was synthesized according to the method described in Example 12A by coupling of 2-(3(S)-amino-2(R)-hydroxy-4-phenylbutyl)-N-tert-butyldecahydro-(4aS,8aS)-isoquinoline-3(S)-carboxamide with N-methyl-N-[(((2-isopropyl-4-thiazolyl)methyl)amino)carbonyl]-L-valine which was prepared according to the method described in J. Org Chem., 1994, 59, 3656-3664.

[0250] Elemental analysis: Theory: C: 65.52, H: 8.62, N: 12.07 Found: C: 64.50, H: 8.72, N: 12.09

EXAMPLE 32

N′-[(1S)-1-[[[(1S,2R)-3-[(2S,4R)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethoxy)-1-piperidinyl]-2-hydroxy-1-(phenylmethyl)p ropyl]amino]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea

[0251] 32A. 1-cis-N-(1,1-Dimethylethyl)-1-(1,1-dimethylethyloxycarbonyl)-4-(5-thiazolylmethyloxy)piperidine-2-carboxamide.

[0252] 1-cis-N-(1,1-Dimethylethyl)-1-(1,1-dimethylethyloxycarbonyl)-4-hydroxy-piperidine-2-carboxamide was prepared according to the methods described in EP 560 268 A1. The hydroxy-carboxamide compound was treated with sodium hydride and 5-thiazolyl methyl chloride in DMF at 0° C. to provide the title compound.

[0253] 32B. 1-cis N-(1,1-Dimethylethyl)-4-(5-thioazoylmethyloxy)piperidine-2-carboxamide.

[0254] A solution of 1.24 g of 1-cis-N-(1,1-dimethylethyl)-1-(1,1-dimethylethyloxycarbonyl)-4-(5-thiazolylmethyloxy)piperidine-2-carboxamide in 10 ml of THF was prepared. The mixture was treated with 3 N HCl, and stirred at room temperature for 1 hour. After stirring, the reaction was it was partitioned between methylene chloride and saturated sodium bicarbonate. The organic layer was washed with brine, dried with Na2SO4 and evaporated to dryness to provide 836 mg of desired product.

[0255] 32C. N′-[(1S)-1-[[[(1S,2R)-3-[(2S,4R)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethoxy)-1-piperidinyl]-2-hydroxy-1-(phenylmethyl)prop yl]amine.

[0256] The title compound was synthesized in two steps. N′-[(1S)-1-[[[(1S,2R)-3-[(2S,4R)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethoxy)-1-piperidinyl]-2-hydroxy-1-(phenylmethyl)prop yl]amine was prepared according to the method described in Example 1A by using t-butyloxycarbonyl phenylalanylepoxide replacing N-benzyloxycarbonyl phenylalanylepoxide followed by column purification. The resulting product was treated with trifluoroacetic acid (TFA) in methylene chloride at room temperature.

[0257] 32D. N′-[(1S)-1-[[[(1S,2R)-3-[(2S,4R)-2-[[(1,1-Dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethoxy)-1-piperidinyl]-2-hydroxy-1-(phenylmethyl)prop yl]amino]carbonyl]-2-methylpropyl]-N-methyl-N-[2-(1-methylethyl)-4-thiazolylmethyl]urea.

[0258] The title compound was synthesized by the method described in Example 12A using N′-[(1S)-1-[[[(1S,2R)-3-[(2S,4R)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethoxy)-1-piperidinyl]-2-hydroxy-1-(phenylmethyl)prop yl]amine and N-methyl-N-[(((2-isopropyl-4-thiazolyl)methyl)amino)carbonyl]-L-valine.

[0259] MS: 756 (M+H)+.

[0260] 1H NMR (CDCl3) d 0.65 (d, J=6.3 Hz, 3H), 0.92 (d, J=6.3 Hz, 3H), 1.32 (d, J=3.0 HZ, 3H), 1.34 (d, J=3.0 Hz, 2H), 1.35 (s, 9H), 1.53 (m, 1H), 1.58 (m, 1H), 1.91 (m, 1H), 2.20 - 2.37 (m, 4H), 2.67 (m, 1H), 2.69 (m, 1H), 2.80 (dd, J=11.4, 3.0 Hz, 1H), 2.87 (dd, J=15.0, 3.3 Hz, 1H), 2.97 (s, 3H), 3.21 (heptet, J=6.3 Hz, 1H), 3.40 (t, J=4.5 Hz, 1H), 3.44 (m, 1H), 3.91 (t, J=5.7 Hz, 1H), 4.03 (m, 2H), 4.12 (m, 1H), 4.35 (AB, J=15.0 Hz, 2H), 4.78 (AB, J=12.0 Hz, 2H), 6.48 (d, J=8.1 Hz, 1H), 6.61 (s, 1H), 7.00 (s, 1H), 7.09-7.18 (m, 6H), 7.78 (s, 1H), 8.79 (s, 1H).

EXAMPLE 33

2-(1-Methylethyl)-4-thiazolylmethyl [(1S)-1-[[[(1S,2R)-3-[(2S,4R)-2-[[(1,1-dimethylethyl)amino]carbonyl]-4-(5-thiazolylmethoxy)-1-piperidinyl]-2-hydroxy-1-(phenylmethyl)propyl] amino]carbonyl]-2-methylpropyl]carbamate

[0261] The title compound is prepared according to the procedure described in example 32C using N-[(2-isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine in place of N-methyl-N-[(((2-isopropyl-4-thiazolyl)methyl)amino)carbonyl]-L-valine as a coupling reagent.

EXAMPLE 34

S-[2-(1-Methylethyl)-4-thiazolylmethyl] [(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,3-benzodioxol-5-ylmethyl)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)pr opyl]amino]carbonyl]-2-methylpropyl]carbamothioate

[0262] 34A. 2-Isopropyl-4-(methanesulfonyloxymethyl)thiazole.

[0263] A solution of 1.2 mmol of 4-(hydroxymethyl)-2-isopropylthiazole and 1.3 mmol of diisopropylethylamine in 20 ml of dichloromethane is cooled to −20° C. and treated dropwise with 1.3 mmol of methanesulfonyl chloride. The resulting mixture is stirred for 1 hour, quenched with aqueous citric acid, separated, dried over Na2SO4, and concentrated in vacuo to provide the title compound.

[0264] 34B. 2-Isopropyl-4-(mercaptomethyl)thiazole.

[0265] A mixture of 0.8 mmol of the product prepared in Example 34A and 1.0 mmol of sodium hydrosulfide hydrate in 20 ml of THF is heated at reflux until analysis by thin layer chromatography indicates consumption of the starting material. The resulting mixture is allowed to cool, concentrated in vacuo, partitioned between dichloromethane and water, dried over Na2SO4, and concentrated to provide the crude compound.

[0266] 34C. N-((2-Isopropyl-4-thiazolyl)thiomethoxycarbonyl)valine methyl ester.

[0267] A solution of 2.18 g (15 mmol) of 2-Isopropyl-4-(mercaptomethyl)thiazole, 15.8 mmol of α-isocyanato-valine methyl ester and 1.5 mmol of 4-dimethylaminopyridine in 75 ml of dichloromethane is heated at reflux for about 5 hours. The resulting solution is washed successively with 10% citric acid, aqueous NaHCO3 and brine, dried over Na2SO4, and concentrated ion vacuo. Silica gel chromatography of the residue using 5% ethyl acetate in chloroform will provide the title compound.

[0268] 34D. N-((2-Isopropyl-4-thiazolyl)thiomethoxycarbonyl)valine.

[0269] A solution of product, prepared in Example 34C in dioxane is treated with 0.50 M aqueous LiOH. The resulting solution is stirred at ambient temperature for about 30 minutes, treated with 1 M HCl, and concentrated in vacuo. The residue is taken up in dichloromethane, washed with water, dried over Na2SO4, and concentrated in vacuo to provide the title compound.

[0270] 34E. S-[2-(1-Methylethyl)-4-thiazolylmethyl [(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl-4-[[(1,1-dimethylethyl)carbonyl]oxy]-1-piperazinyl]-2-hydroxy-1-(phenylmeth yl)propyl]amino]carbonyl]-2-methylpropyl]carbamothioate.

[0271] The title compound is prepared according to the method described in Example 10, substituting N-((2-Isopropyl-4-thiazolyl)thiomethoxycarbonyl)valine in place of [(2-isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine.

[0272] 34F. S-[2-(1-Methylethyl)-4-thiazolylmethyl[(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylme thyl)propyl]amino]carbonyl]-2-methylpropyl]carbamothioate.

[0273] The title compound is prepared according to the method described in Example 1D, substituting S-[2-(1-methylethyl)-4-thiazolylmethyl] [(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,1-dimethylethyloxy(carbonyl))-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethy l)propyl]amino]carbonyl]-2-methylpropyl]carbamothioate in place of 2-(1-methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,1-dimethylethyloxy(carbonyl))-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-pi perazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0274] 34G. S-[2-(1-Methylethyl)-4-thiazolylmethyl][(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,3-benzodioxol-5-ylmethyl)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-pi perazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamothioate

[0275] The title compound is prepared according to the method described in Example 1E, substituting S-[2-(1-methylethyl)-4-thiazolylmethyl] [(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpr opyl]carbamothioate in place of 2-(1-methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethy l)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

EXAMPLE 35

4-(1,3-Benzodioxol-5-ylmethyl)-N-(1,1-dimethylethyl)-1-[(2R,3S)-3-[[(2S)-2-[[3-[2-(1-methylethyl)-4-thiazolyl]-1-oxopropyl]amino]-3-methy l-1-oxobutyl]amino]-2-hydroxy-4-phenylbutyl]-2-piperazinecarboxamide

[0276] 35A. 2-Isopropylthiazole-4-carboxaldehyde.

[0277] A solution of 3.1 g (15.6 mmol) of ethyl 2-isopropylthiazole-4-carboxylate in 50 ml of dichloromethane was cooled under N2 atmosphere to −78° C. and treated dropwise with 15.6 ml (23.4 mmol) of a 1.5 M solution of diisobutylaluminum hydride in toluene over a period of 1.5 h. After being stirred for an additional 0.5 h, the solution was quenched with 5 ml of methanol followed by 15 ml of aqueous Rochelle's salt. The resulting mixture was partitioned between chloroform and aqueous Rochelle's salt, dried over Na2SO4, and concentrated to provide 1.37 g (56%) of the crude desired compound, Rf 0.47 (20% ethyl acetate in hexane).

[0278] 1H NMR (CDCl3) d 1.45 (d, J=7 Hz, 6 H), 3.39 (heptet, J=7 Hz, 1H), 8.07 (s, 1H), 10.00 (s, 1H).

[0279] Mass spectrum: (M+H)+=156.

[0280] 35B. (E)-Ethyl 3-(2-Isopropyl-4-thiazolyl)propenoate.

[0281] A slurry of 60% NaH (18 mmol) in mineral oil was washed with hexane, decanted under N2 atmosphere, and diluted with 25 ml of THF. The resulting mixture was cooled to 0° C., treated portionwise with 3.24 ml (16.4 mmol) of triethylphosphonoacetate. After addition, the solution was stirred for 10 minutes, treated with 1.37 g (8.84 mmol) of 2-isopropylthiazole-4-carboxaldehyde in 25 ml of THF, allowed to warm to ambient temperature for 25 minutes, and quenched with 100 ml of saturated aqueous NH4Cl. The mixture was extracted with three 100 ml portions of ethyl acetate, dried over Na2SO4, and concentrated in vacuo, Silica gel chromatography of the residue using 5-10% ethyl acetate in hexane provided 1.61 g (81%) of the desired compound, Rf 0.64 (20% ethyl acetate in hexane).

[0282] 1H NMR (CDCl3) d 1.33 (t, J=7 Hz, 3 H), 1.42 (d, J=7 Hz, 6 H), 3.32 (heptet, J=7 Hz, 1H), 4.26 (q, J=7 Hz, 2 H), 6.75 (d, J=15 Hz, 1H), 7.29 (s, 1H), 7.57 (d, J=15 Hz, 1H).

[0283] 35C. Methyl 3-(2-Isopropyl-4-thiazolyl)propanoate.

[0284] A solution of 225 mg (1 mmol) of (E)-ethyl 3-(2-isopropyl-4-thiazolyl)propenoate in 10 ml of freshly distilled (from calcium hydride) methanol and 1 ml of dry THF is treated with 49 mg (2 mmol) of magnesium turnings. The mixture is stirred for 20 minutes, and the magnesium is consumed. The resulting solution is poured over cold aqueous HCl, basified to pH 8 with NaHCO3, extracted with ethyl acetate, dried over Na2SO4, and concentrated. Silica gel chromatography using 10% ethyl acetate in hexane provides a mixture of the desired compound and methyl 3-(2-isopropyl-4-thiazolinyl)propanoate.

[0285] 35D. 3-(2-Isopropyl-4-thiazolyl)propanoic Acid.

[0286] A solution of product, prepared in Example 35C in dioxane is treated with 0.50 M aqueous LiOH. The resulting solution is stirred at ambient temperature for about 30 minutes, treated with 8.7 ml of 1 M HCl, and concentrated in vacuo. The residue is taken up in dichloromethane, washed with water, dried over Na2SO4, and concentrated in vacuo to provide the title compound.

[0287] 35E. N-(1,1-Dimethylethyl)-4-[[(1,1-dimethylethyl)carbonyl]oxy]-1-[(2R,3S)-3-[[(2S)-2-[[3-[2-(1-methylethyl)-4-thiazolyl]-1-oxopropyl]amino]-3-me thyl-1-oxobutyl]amino]-2-hydroxy-4-phenylbutyl]-2-piperazinecarboxamide.

[0288] A solution of 3-(2-isopropyl-4-thiazolyl)propanoic acid, 1,1 equivalents of α-isocyanato-valine methyl ester and 4-dimethylaminopyridine (catalytic) in of dichloromethane is heated at reflux for about 5 hours. The resulting solution is washed successively with 10% citric acid, aqueous NaHCO3 and brine, dried over Na2SO4, and concentrated in vacuo. Silica gel chromatography of the residue using 5% ethyl acetate in chloroform to provide the title compound.

[0289] 35F. N-(1,1-Dimethylethyl)-1-[(2R,3S)-3-[[(2S)-2-[[3-[2-(1-methylethyl)-4-thiazolyl]-1-oxopropyl]amino]-3-methyl-1-oxobutyl]amino]-2-hydroxy-4-ph enylbutyl]-2-piperazinecarboxamide.

[0290] The title compound is prepared according to the method described in Example 1C, substituting N-((2-Isopropyl-4-thiazolyl)propionyl)valine in place of [(2-isopropyl-4-thiazolyl)methyloxycarbonyl]-L-valine.

[0291] 35G. N-(1,1-Dimethylethyl)-1-[(2R,3S)-3-[[(2S)-2-[[3-[2-(1-methylethyl)-4-thiazolyl]-1-oxopropyl]amino]-3-methyl-1-oxobutyl]amino]-2-hydroxy-4-ph enylbutyl]-2-piperazinecarboxamide.

[0292] The title compound is prepared according to the method described in Example 1D, substituting 4-(1,1-dimethylethyloxy(carbonyl))-N-(1,1-dimethylethyl)-1-[(2R ,3S)-3-[[(2S)-2-[[3-[2-(1-methylethyl)-4-thiazolyl]-1-oxopropyl]amino]-3-methyl-1-oxobutyl]amino]-2-hydroxy-4-phenylbutyl]-2-piperazinecarbo xamide in place of 2-(1-methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-4-(1,1-dimethylethyloxy(carbonyl))-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-pi perazinyl]-2-hydroxy-1-(phenylmethyl)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

[0293] 35H. 4-(1,3-Benzodioxol-5-ylmethyl)-N-(1,1-dimethylethyl)-1-[(2R,3S)-3-[[(2S)-2-[[3-[2-(1-methylethyl)-4-thiazolyl-1-oxopropyl]amino]-3-methyl-1- oxobutyl]amino]-2-hydroxy-4-phenylbutyl]-2-piperazinecarboxamide.

[0294] The title compound is prepared according to the method described in Example 1E, substituting N-(1,1-dimethylethyl)-1-[(2R,3S)-3-[[(2S)-2-[[3-[2-(1-methylethyl)-4-thiazolyl]-1-oxopropyl]amino]-3-methyl-1-oxobutyl]amino]-2-hydroxy-4-ph enylbutyl]-2-piperazinecarboxamide in place of 2-(1-methylethyl)-4-thiazolylmethyl-(1S)-1-[[[(1S,2R)-3-[(2S)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinyl]-2-hydroxy-1-(phenylmethy l)propyl]amino]carbonyl]-2-methylpropyl]carbamate.

Fluorogenic Assay for Screening Inhibitors of HIV Protease

[0295] The inhibitory potency of the compounds of the invention can be determined by the following method:

[0296] A compound of the invention is dissolved in DMSO. A small aliquot is diluted with DMSO to 100 times the final concentration desired for testing. The test is carried out in a 6×50 mm tube in a total volume of 300 microliters. The final concentrations of the components in the reaction buffer are: 125 mM sodium acetate, 1 M sodium chloride, 5 mM dithiothreitol, 0.5 mg/ml bovine serum albumin, 1.3 μM fluorogenic substrate, 2% (v/v) dimethylsulfoxide, pH 4.5. After addition of inhibitor, the reaction mixture is placed in the fluorometer cell holder and incubated at 30° C. for several minutes. The reaction is initiated by the addition of a small aliquot of cold HIV protease. The fluorescence intensity (excitation 340 nM, emmision 490 nM) is recorded as a function of time. The reaction rate is determined for the first six to eight minutes. The observed rate is directly proportional to the moles of substrate cleaved per unit time. The percent inhibition is 100×(1- (rate in presence of inhibitor)/(rate in absence of inhibitor)).

[0297] Fluorogenic substrate: Dabcyl-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS wherein DABCYL=4-(4-dimethylaminophenyl)azobenzoic acid and EDANS=5-((2-aminoethyl)amino)naphthalene-1-sulfonic acid. All compounds tested at 1.0 nM were found to have an IC50 of 100%. The test compounds were then tested at a concentration of 0.5 nM. The results are reported as percent inhibition, in Table 1, below. 1

TABLE 1
Example No.% Inhib. (0.5 nM)
Example 1 60%
Example 2 66%
Example 3 64%
Example 4 67%
Example 5 69%
Example 6 60%
Example 7 73%
Example 8 54%
Example 9 65%
Example 1070%
Example 1162%
Example 1261%
Example 1366%
Example 1463%
Example 1545%
Example 1649%
Example 1750%
Example 1882%
Example 1951%
Example 2037%
Example 2144%
Example 2351%
Example 2440%
Example 2545%
Example 2642%
Example 2750%
Example 2877%
Example 2964%
Example 3055%
Example 3148%
Example 3266%

[0298] Table 1 shows the inhibitory potencies of compounds of the invention against HIV-1 protease.

Antiviral Activity

[0299] The anti-HIV activity of the compounds of the invention can be determined in MT4 cells according to the procedure of Kempf, et. al. (Antimicrob. Agents Chemother. 1991, 35, 2209). The IC50 is the concentration of compound that gives 50% inhibition of the cytopathic effect of HIV. The LC50 is the concentration of compound at which 50% of the cells remain viable. 2

TABLE 2
Example No.EC50(nM)LC50(μM)
Example 1 312.76
Example 2 2247.11
Example 3 2115.73
Example 4 1268.95
Example 5 4016.70
Example 6 1258.61
Example 7 1755.20
Example 8 4718.90
Example 9 4618.45
Example 101418.18
Example 111718.33
Example 121370.34
Example 134860.35
Example 144122.74
Example 151153.09
Example 161061.73
Example 171139.17
Example 181944.64
Example 191720.69
Example 201649.74
Example 211153.05
Example 23259>100
Example 241755.42
Example 2557>100
Example 2681717.35
Example 2737420.60
Example 281149.52
Example 2917531.15
Example 302017.87
Example 315817.22
Example 321564.14

[0300] The compounds of the present invention can be used in the form of salts derived from inorganic or organic acids. These salts include but are not limited to the following: acetate, adipate, alginate, citrate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, camphorate, camphorsulfonate, digluconate, cyclopentanepropionate, dodecylsulfate, ethanesulfonate, glucoheptanoate, glycerophosphate, hemisulfate, heptanoate, hexanoate, fumarate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethanesulfonate (isethionate), lactate, maleate, methanesulfonate, nicotinate, 2-naphthalenesulfonate, oxalate, pamoate, pectinate, persulfate, 3-phenylpropionate, picrate, pivalate, propionate, succinate, tartrate, thiocyanate, p-toluenesulfonate and undecanoate. Also, the basic nitrogen-containing groups can be quaternized with such agents as lower alkyl halides, such as methyl, ethyl, propyl, and butyl chloride, bromides, and iodides; dialkyl sulfates like dimethyl, diethyl, dibutyl, and diamyl sulfates, long chain halides such as decyl, lauryl, myristyl and stearyl chlorides, bromides and iodides, aralkyl halides like benzyl and phenethyl bromides, and others. Water or oil-soluble or dispersible products are thereby obtained.

[0301] Examples of acids which may be employed to form pharmaceutically acceptable acid addition salts include such inorganic acids as hydrochloric acid, sulfuric acid and phosphoric acid and such organic acids as oxalic acid, maleic acid, succinic acid and citric acid. Other salts include salts with alkali metals or alkaline earth metals, such as sodium, potassium, calcium or magnesium or with organic bases.

[0302] Preferred salts of the compounds of the invention include hydrochloride, methanesulfonate, sulfonate, phosphonate and isethionate.

[0303] The compounds of the present invention can also be used in the form of esters. Examples of such esters include a hydroxyl-substituted compound of formula I or II which has been acylated with a blocked or unblocked amino acid residue, a phosphate function, a hemisuccinate residue, an acyl residue of the formula R14C(O)— or R14C(S)— wherein R14 is hydrogen, lower alkyl, haloalkyl, alkoxy, thioalkoxy, alkoxyalkyl, thioalkoxyalkyl or haloalkoxy, or an acyl residue of the formula Ra—C(Rb)(Rd)—C(O)— or Ra—C(Rb)(Rd)—C(S)— wherein Rb and Rd are independently selected from hydrogen or lower alkyl and Ra is —N(Re)(Rf), ORe or —SRe wherein Re and Rf are independently selected from hydrogen, lower alkyl and haloalkyl, or an amino-acyl residue having the formula R15NH(CH2)2NHCH2C(O)— or R15NH(CH2)2OCH2C(O)— wherein R15 is hydrogen, lower alkyl, arylalkyl, cycloalkylalkyl, alkanoyl, benzoyl or an α-amino acyl group. The amino acid esters of particular interest are glycine and lysine; however, other amino acid residues can also be used, including those wherein the amino acyl group is —C(O)CH2NR16R17 wherein R16 and R17 are independently selected from hydrogen and lower alkyl, or the group —NR16R17, where R16R17, taken together, forms a nitrogen containing heterocyclic ring. These esters serve as pro-drugs of the compounds of the present invention and serve to increase the solubility of these substances in the gastrointestinal tract. These esters also serve to increase solubility for intravenous administration of the compounds. Other prodrugs include a hydroxyl-substituted compound of formula I or II wherein the hydroxyl group is functionalized with a substituent of the formula —CH(R18)OC(O)R19 or —CH(R18)OC(S)R19 wherein R19 is lower alkyl, haloalkyl, alkoxy, thioalkoxy or haloalkoxy and R18 is hydrogen, lower alkyl, haloalkyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl or dialkylaminocarbonyl. Such prodrugs can be prepared according to the procedure of Schreiber (Tetrahedron Lett. 1983, 24, 2363) by ozonolysis of the corresponding methallyl ether in methanol followed by treatment with acetic anhydride.

[0304] The prodrugs of this invention are metabolized in vivo to provide the hydroxyl-substituted compound of formula I or II. The preparation of the prodrug esters is carried out by reacting a hydroxyl-substituted compound of formula I or II with an activated amino acyl, phosphoryl, hemisuccinyl or acyl derivative as defined above. The resulting product is then deprotected to provide the desired pro-drug ester. Prodrugs of the invention can also be prepared by alkylation of the hydroxyl group with (halo)alkyl esters, transacetalization with bis-(alkanoyl)acetals or condensation of the hydroxyl group with an activated aldehyde followed by acylation of the intermediate hemiacetal.

[0305] The compounds of the invention are useful for inhibiting retroviral protease, in particular HIV protease, in vitro or in vivo (especially in mammals and in particular in humans). The compounds of the present invention are also useful for the inhibition of retroviruses in vivo, especially human immunodeficiency virus (HIV). The compounds of the present invention are also useful for the treatment or prophylaxis of diseases caused by retroviruses, especially acquired immune deficiency syndrome or an HIV infection in a human or other mammal.

[0306] Total daily dose administered to a human or other mammal host in single or divided doses may be in amounts, for example, from 0.001 to 300 mg/kg body weight daily and more usually 0.1 to 10 mg. Dosage unit compositions may contain such amounts of submultiples thereof to make up the daily dose.

[0307] The amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration.

[0308] It will be understood, however, that the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, rate of excretion, drug combination, and the severity of the particular disease undergoing therapy.

[0309] The compounds of the present invention may be administered orally, parenterally, sublingually, by inhalation spray, rectally, or topically in dosage unit formulations containing conventional nontoxic pharmaceutically acceptable carriers, adjuvants, and vehicles as desired. Topical administration may also involve the use of transdermal administration such as transdermal patches or iontophoresis devices. The term parenteral as used herein includes subcutaneous injections, intravenous, intramuscular, intrasternal injection, or infusion techniques.

[0310] Injectable preparations, for example, sterile injectable aqueous or oleagenous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution or suspension in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-propanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid find use in the preparation of injectables.

[0311] Suppositories for rectal administration of the drug can be prepared by mixing the drug with a suitable nonirritating excipient such as cocoa butter and polyethylene glycols which are solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum and release the drug.

[0312] Solid dosage forms for oral administration may include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound may be admixed with at least one inert diluent such as sucrose lactose or starch. Such dosage forms may also comprise, as is normal practice, additional substances other than inert diluents, e.g., lubricating agents such as magnesium stearate. In the case of capsules, tablets, and pills, the dosage forms may also comprise buffering agents. Tablets and pills can additionally be prepared with enteric coatings.

[0313] Liquid dosage forms for oral administration may include pharmaceutically acceptable emulsions, solutions, suspensions, syrups, and elixirs containing inert diluents commonly used in the art, such as water. Such compositions may also comprise adjuvants, such as wetting agents, emulsifying and suspending agents, and sweetening, flavoring, and perfuming agents.

[0314] The compounds of the present invention can also be administered in the form of liposomes. As is known in the art, liposomes are generally derived from phospholipids or other lipid substances. Liposomes are formed by mono- or multi-lamellar hydrated liquid crystals that are dispersed in an aqueous medium. Any non-toxic, physiologically acceptable and metabolizable lipid capable of forming liposomes can be used. The present compositions in liposome form can contain, in addition to a compound of the present invention, stabilizers, preservatives, excipients, and the like. The preferred lipids are the phospholipids and phosphatidyl cholines (lecithins), both natural and synthetic.

[0315] Methods to form liposomes are known in the art. See, for example, Prescott, Ed., Methods in Cell Biology, Volume XIV, Academic Press, New York, N.Y. (1976), p. 33 et seq.

[0316] While the compounds of the invention can be administered as the sole active pharmaceutical agent, they can also be used in combination with one or more immunomodulators, antiviral agents, other antiinfective agents or vaccines. Other antiviral agents to be administered in combination with a compound of the present invention include AL-721, beta interferon, polymannoacetate, reverse transcriptase inhibitors (for example, dideoxycytidine (DDC), dideoxyinosine (DDI), BCH-189, AzdU, carbovir, DDA, D4C, D4T, DP-AZT, FLT (fluorothymidine), BCH-189, 5-halo-3′-thiadideoxycytidine, PMEA, zidovudine (AZT) and the like), non-nucleoside reverse transcriptase inhibitors (for example, R82193, L-697,661, BI-RG-587 (nevirapine), DMP-266, and the like) retroviral protease inhibitors (for example, HIV protease inhibitors such as ritonavir, ABT-378, saquinavir, nelfinavir, indinavir, VX-478 (amprenavir), SC-52151, KNI-227, KNI-272, U-140690, DMP-450, and the like), HEPT compounds, L,697,639, R82150, U-87201 E and the like), TAT inhibitors (for example, RO-24-7429 and the like), trisodium phosphonoformate, HPA-23, eflonithine, Peptide T, Reticulose (nucleophosphoprotein), ansamycin LM 427, trimetrexate, UA001, ribavirin, alpha interferon, oxetanocin, oxetanocin-G, cylobut-G, cyclobut-A, ara-M, BW882C87, foscarnet, BW256U87, BW348U87, L-693,989, BV ara-U, CMV triclonal antibodies, FIAC, HOE-602, HPMPC, MSL-109, TI-23, trifluridine, vidarabine, famciclovir, penciclovir, acyclovir, ganciclovir, castanospermine, rCD4/CD4-IgG, CD4-PE40, butyl-DNJ, hypericin, oxamyristic acid, dextran sulfate and pentosan polysulfate. Immunomodulators that can be administered in combination with a compound of the present invention include bropirimine, Ampligen, anti-human alpha interferon antibody, colony stimulting factor, CL246,738, Imreg-1, Imreg-2, diethydithiocarbamate, interleukin-2, alpha-interferon, inosine pranobex, methionine enkephalin, muramyl-tripeptide, TP-5, erythropoietin, naltrexone, tumor necrosis facator, beta interferon, gamma interferon, interleukin-3, interleukin-4, autologous CD8+infusion, alpha interferon immunoglobulin, IGF-1, anti-Leu-3A, autovaccination, biostimulation, extracorporeal photophoresis, FK-565, FK-506, G-CSF, GM-CSF, hyperthermia, isopinosine, IVIG, HIVIG, passive immunotherapy and polio vaccine hyperimmunization. Other antiinfective agents that can be administered in combination with a compound of the present invention include pentamidine isethionate. Any of a variety of HIV or AIDS vaccines (for example, gp120 (recombinant), Env 2-3 (gp120), HIVAC-1e (gp120), gp160 (recombinant), VaxSyn HIV-1 (gp160), Immuno-Ag (gp160), HGP-30, HIV-Immunogen, p24 (recombinant), VaxSyn HIV-1 (p24) can be used in combination with a compound of the present invention.

[0317] Other agents that can be used in combination with the compounds of this invention are ansamycin LM 427, apurinic acid, ABPP, Al-721, carrisyn, AS-101, avarol, azimexon, colchicine, compound Q, CS-85, N-acetyl cysteine, (2-oxothiazolidine-4-carboxylate), D-penicillamine, diphenylhydantoin, EL-10, erythropoieten, fusidic acid, glucan, HPA-23, human growth hormone, hydroxchloroquine, iscador, L-ofloxacin or other quinolone antibiotics, lentinan, lithium carbonate, MM-1, monolaurin, MTP-PE, naltrexone, neurotropin, ozone, PAI, panax ginseng, pentofylline, pentoxifylline, Peptide T, pine cone extract, polymannoacetate, reticulose, retrogen, ribavirin, ribozymes, RS-47, Sdc-28, silicotungstate, THA, thymic humoral factor, thymopentin, thymosin fraction 5, thymosin alpha one, thymostimulin, UA001, uridine, vitamin B12 and wobemugos.

[0318] Other agents that can be used in combination with the compounds of this invention are antifungals such as amphotericin B, clotrimazole, flucytosine, fluconazole, itraconazole, ketoconazole and nystatin and the like.

[0319] Other agents that can be used in combination with the compounds of this invention are antibacterials such as amikacin sulfate, azithromycin, ciprofloxacin, tosufloxacin, clarithromycin, clofazimine, ethambutol, isoniazid, pyrazinamide, rifabutin, rifampin, streptomycin and TLC G-65 and the like.

[0320] Other agents that can be used in combination with the compounds of this invention are anti-neoplastics such as alpha interferon, COMP (cyclophosphamide, vincristine, methotrexate and prednisone), etoposide, mBACOD (methotrexate, bleomycin, doxorubicin, cyclophosphamide, vincristine and dexamethasone), PRO-MACE/MOPP(prednisone, methotrexate (w/leucovin rescue), doxorubicin, cyclophosphamide, etoposide/mechlorethamine, vincristine, prednisone and procarbazine), vincristine, vinblastine, angioinhibins, pentosan polysulfate, platelet factor 4 and SP-PG and the like.

[0321] Other agents that can be used in combination with the compounds of this invention are drugs for treating neurological disease such as peptide T, ritalin, lithium, elavil, phenytoin, carbamazipine, mexitetine, heparin and cytosine arabinoside and the like.

[0322] Other agents that can be used in combination with the compounds of this invention are anti-protozoals such as albendazole, azithromycin, clarithromycin, clindamycin, corticosteroids, dapsone, DIMP, eflornithine, 566C80, fansidar, furazolidone, L,671,329, letrazuril, metronidazole, paromycin, pefloxacin, pentamidine, piritrexim, primaquine, pyrimethamine, somatostatin, spiramycin, sulfadiazine, trimethoprim, TMP/SMX, trimetrexate and WR 6026 and the like.

[0323] Among the preferred agents for treatment of HIV or AIDS in combination with the compounds of this invention are reverse transcriptase inhibitors and other HIV protease inhibitors.

[0324] It will be understood that agents which can be combined with the compounds of the present invention for the treatment or prophylaxis of AIDS or an HIV infection are not limited to those listed above, but include in principle any agents useful for the treatment or prophylaxis of AIDS or an HIV infection.

[0325] When administered as a combination, the therapeutic agents can be formulated as separate compositions which are given at the same time or different times, or the therapeutic agents can be given as a single composition.

[0326] The foregoing is merely illustrative of the invention and is not intended to limit the invention to the disclosed compounds. Variations and changes which are obvious to one skilled in the art are intended to be within the scope and nature of the invention which are defined in the appended claims.