Name: Proceedings of the North Dakota Academy of Science Publisher: North Dakota Academy of Science Audience: Academic Format: Magazine/Journal Subject: Science and technology Copyright: COPYRIGHT 2009 North Dakota Academy of Science ISSN:0096-9214
Issue:
Date: April, 2009 Source Volume: 63
Geographic:
Geographic Scope: United States Geographic Code: 1USA United States
Accession Number:
199067587
Full Text:
We describe a multiplex electrochemical immunoassay based on the
use of gold nanoparticle (Au-NP) probes and immunochromatographic strips
(ISs). The approach takes advantage of the speed and low cost of the
conventional IS tests and the high sensitivities of the
nanoparticle-based electrochemical immunoassays. Rabbit IgG (R-IgG) and
human IgM (H-IgM) were used as model targets for the demonstration of
the proof concept. The Au-NPs based sandwich immunoreactions were
performed on the IS, and the captured gold nanoparticle labels on the
test zones were determined by highly sensitive stripping voltammetric
measurement of the dissolved gold ions (III) with a carbon paste
electrode. The detection limits are 1.0 and 1.5 ng [ml.sup.-1] with the
linear range of 2.5-250 ng [ml.sup.-1] for quantitative detection of
R-IgG and H-IgM, respectively. The total assay time is around 25 min.
Such multiplex electrochemical immunoassay could be readily highly
multiplexed to allow simultaneous parallel detection of numerous
proteins and is expected to open new opportunities for protein
diagnostics and biosecurity.
[FIGURE 1 OMITTED]
Xun Mao (a), Meenu Baloda (a), Anant S. Gurung (a), Yuehe Lin (b),
Guodong Liu (a), *
(a) Department of Chemistry and Molecular Biology, North Dakota
State University, Fargo, ND 58105, (b) pacific Northwest National
Laboratory, Richland, WA 99352
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