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EP1944310A2 |
Modified oligonucleotides for mismatch discrimination
Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor...
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7393645 |
Compositions for the electronic detection of analytes utilizing monolayers
The present invention relates to the use of self-assembled monolayers with mixtures of conductive oligomers and insulators to detect target analytes.
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7384749 |
Electrodes linked via conductive oligomers to nucleic acids
The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids...
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7381533 |
Electrodes linked via oligomers to nucleic acids
The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids...
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7381525 |
AC/DC voltage apparatus for detection of nucleic acids
The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids...
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7368549 |
Tm leveling compositions
Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor...
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7348146 |
Single nucleotide polymorphism analysis of highly polymorphic target sequences
Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.
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7338805 |
Labeling reagents, methods for synthesizing such reagents and methods for detecting biological molecules
The present invention relates to a temperature-stable labeling reagent of formula:
in which
R 1 represents H or an alkyl, aryl or substituted aryl group, R 2 represents a detectable...
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7312087 |
Devices and methods for biochip multiplexing
The invention is directed to devices and methods that allow for simultaneous multiple biochip analysis. The method of analyzing the plurality of biochips includes inserting a first biochp into a...
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7297518 |
Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
The invention provides methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension. Some applications of the invention utilize total internal reflection fluorescence...
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7267939 |
Detection of analytes using reorganization energy
The invention relates to novel methods and compositions for the detection of analytes using the nuclear reorganization energy, λ, of an electron transfer process.
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7220549 |
Stabilizing a nucleic acid for nucleic acid sequencing
The invention provides methods for sequencing a nucleic acid comprising stabilizing a primer/target nucleic acid duplex on a substrate. Methods of the invention generally contemplate the use of a...
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7205105 |
Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis
Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5′ nuclease digestion.
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7172897 |
Devices and methods for biochip multiplexing
The invention is directed to devices that allow for simultaneous multiple biochip analysis. In particular, the devices are configured to hold multiple cartridges comprising biochips comprising...
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7169560 |
Short cycle methods for sequencing polynucleotides
The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion.
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7160678 |
Compositions for the electronic detection of analytes utilizing monolayers
The present invention relates to the use of self-assembled monolayers with mixtures of conductive oligomers and insulators to detect target analytes.
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7125668 |
Electrodes linked via conductive oligomers to nucleic acids
The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids...
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7118907 |
Single molecule detection systems and methods
A microfluidic system is provided that includes a substrate, a first microchannel disposed in the substrate for providing a reactant to a reaction zone, a second microchannel disposed in the...
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7090804 |
Amplification of nucleic acids with electronic detection
The invention relates to compositions and methods useful in the detection of nucleic acids using a variety of amplification techniques, including both signal amplification and target amplification....
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7087148 |
Binding acceleration techniques for the detection of analytes
The invention relates to compositions and methods useful in the acceleration of binding of target analytes to capture ligands on surfaces. Detection proceeds through the use of an electron transfer...
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7064197 |
System, array and non-porous solid support comprising fixed or immobilized nucleic acids
Nucleic acids are fixed or immobilized to non-porous solid supports (substrates), and include systems containing such supports and arrays with fixed or immobilized nucleic acids. These compositions...
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7060441 |
Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling
A single- or double-stranded deoxyribonucleic acid (DNA) is fragmented and labeled by chemically fragmenting the DNA by creating at least one abasic site on the DNA, and attaching a marker to at...
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7045610 |
Modified oligonucleotides for mismatch discrimination
Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor...
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7045285 |
Electronic transfer moieties attached to peptide nucleic acids
The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids...
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7033760 |
Metallic solid supports modified with nucleic acids
The present invention is directed to solid supports having metallic surfaces comprising blocking moieties and modified nucleic acids, which exhibit excellent characteristics in hybridization...
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7018523 |
Detection of analytes using reorganization energy
The invention relates to novel methods and compositions for the detection of analytes using the nuclear reorganization energy, λ, of an electron transfer process.
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7014992 |
Conductive oligomers attached to electrodes and nucleoside analogs
The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids...
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6949367 |
Modified oligonucleotides for mismatch discrimination
Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor...
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6942771 |
Microfluidic systems in the electrochemical detection of target analytes
The invention relates generally to methods and apparatus for conducting analyses, particularly microfluidic devices for the detection of target analytes.
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6921640 |
Process for labeling a ribonucleic acid
The present invention relates to a process for labeling a synthetic or natural ribonucleic acid (RNA). It also relates to RNA fragments, which have been labeled by fragmenting the RNA to free a...
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6911345 |
Methods and apparatus for analyzing polynucleotide sequences
The present invention provides an apparatus for analyzing the sequences of polynucleotides. The apparatus comprises (a) flow cell which has at least one microfabricated multilayer elastomeric...
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6902891 |
Process for labeling a nucleic acid
A process of fragmenting and labeling a synthetic or natural nucleic acid, comprising the steps of providing a mixture containing a nucleic acid, a labeling agent containing a detectable label, and...
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6884584 |
Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI 3 ), and an oligonucleotide form unusually stable hybrids with...
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6833267 |
Tissue collection devices containing biosensors
The present disclosure provides tissue collection devices comprising biosensors that can be used for the detection of target analytes, such as nucleic acids and proteins, including antibodies and...
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6818395 |
Methods and apparatus for analyzing polynucleotide sequences
Methods for high speed, high throughput analysis of polynucleotide sequences, and apparatuses with which to carry out the methods are provided in the invention.
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6809196 |
Molecular recognition at surfaces derivatized with self-assembled monolayers
An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres...
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6761816 |
Printed circuit boards with monolayers and capture ligands
The invention relates to compositions and methods useful in the acceleration of binding of target analytes to capture ligands on surfaces. Detection proceeds through the use of an electron transfer...
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6750357 |
Rhodamine-based fluorophores useful as labeling reagents
Fluorescent dyes based on rhodamine are derivatized to form labeled conjugates that fluoresce upon excitation with light of an appropriate wavelength. Particularly preferred embodiments are certain...
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6740518 |
Signal detection techniques for the detection of analytes
The invention relates to the use of signal processing methods in order to acheive higher signal to noise ratios, to increase the detection limits of target analytes. These techniques include the...
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6686150 |
Amplification of nucleic acids with electronic detection
The invention relates to compositions and methods useful in the detection of nucleic acids using a variety of amplification techniques, including both signal amplification and target amplification....
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6683173 |
Tm leveling methods
Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5-substituted pyrimidines, and optionally have attached minor...
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6613516 |
Preparation of nucleic acid samples
The presently claimed invention provides methods, compositions, and apparatus for studying nucleic acids. Specifically, the present invention provides a novel enrichment and labeling strategy for...
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6548259 |
Multiparametric fluorescence in situ hybridization
The invention relates to a set of combinatorially labeled oligonucleotide probes each member thereof: (i) having a predetermined label distinguishable from the label of any other member of said...
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6541617 |
Detection of target analytes using particles and electrodes
The invention relates to the use of particles comprising binding ligands and electron transfer moieties (ETMs). Upon binding of a target analyte, a particle and a reporter composition are...
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6528266 |
Nucleic acid mediated electron transfer
The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties...
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6506563 |
Multiparametric fluorescence in situ hybridization
The invention relates to a set of combinatorially labeled oligonucleotide probes each member thereof: (i) having a predetermined label distinguishable from the label of any other member of said...
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6492346 |
Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI 3 ), and an oligonucleotide form unusually stable hybrids with...
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6489114 |
Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby
A process is provided for labeling with signal amplification a ribonucleic acid (RNA), comprising fragmenting the RNA to form RNA fragments, fixing a first ligand to a terminal phosphate located at...
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6485906 |
Oligonucleotides containing pyrazolo&lsqb 3,4-d&rsqb pyrimidines for hybridization and mismatch discrimination
Oligonucleotides in which one or more purine residues are substituted by pyrazolo[3,4-d]pyrimidines exhibit improved hybridization properties. Oligonucleotides containing pyrazolo[3,4-d]pyrimidine...
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6479240 |
Electrodes linked via conductive oligomers to nucleic acids
The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids...
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