TEMPLATE-MEDIATED, LIGATION-ORIENTED METHOD OF NONRANDOMLY SHUFFLING POLYNUCLEOTIDES
Kind Code:
A1
Abstract not available for EP1381680
Abstract of corresponding document: WO02086121
Method of gene shuffling oriented ligation, whereby at least two fragments are adjacently hybridized on an assembly template. Invention is particularly aimed at generating novel polynucleotides that differ in some advantageous respect compared to a reference sequence. Invention further includes sequences created by the method, hosts and vectors containing same, and proteins translated therefrom.
Abstract of corresponding document: WO02086121
Method of gene shuffling oriented ligation, whereby at least two fragments are adjacently hybridized on an assembly template. Invention is particularly aimed at generating novel polynucleotides that differ in some advantageous respect compared to a reference sequence. Invention further includes sequences created by the method, hosts and vectors containing same, and proteins translated therefrom.
Inventors:
Dupret, Daniel (FR)
Lefevre, Fabrice (FR)
Masson, Jean-michel (FR)
Lefevre, Fabrice (FR)
Masson, Jean-michel (FR)
Application Number:
EP20020743543
Publication Date:
01/21/2004
Filing Date:
04/25/2002
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Assignee:
Proteus (FR)
International Classes:
C12N15/09; C12N15/09; C07K14/00; C07K14/00; C12M1/00; C12M1/00; C12N1/15; C12N1/15; C12N1/19; C12N1/19; C12N1/21; C12N1/21; C12N5/10; C12N5/10; C12N15/10; C12N15/10; C12Q1/68; C12Q1/68; (IPC1-7): C12N15/10; C12Q1/68
European Classes:
C12N15/10B2
Other References:
See references of WO 02086121A1