Title:
Nukleosid-Triphosphat-Konjugate und Methoden zu deren Anwendung
Kind Code:
A1
Abstract:
Die Erfindung beschreibt ein neues Verfahren zur enzymatischen Markierung von Nukleinsäureketten (Zielsequenzen) mit Nukleotid-Konjugaten. Diese Nukleotid-Konjugate sind in der Lage, unter Reaktionsbedingungen an eine Zielsequenz zu binden und durch eine Polymerase in den komplementären wachsenden Strang eingebaut zu werden. Die Nukleotid-Konjugate können zur Sequenzierung von Nukleinsäureketten verwendet werden.


Inventors:
CHERKASOV DMITRY (DE)
BECKER CLAUS PROF DR (DE)
BASLER NORBERT (DE)
MUELLER-HERMANN ANDREAS (DE)
Application Number:
DE102012008759A
Publication Date:
11/08/2012
Filing Date:
05/04/2012
Assignee:
GENOVOXX GMBH
International Classes:
Domestic Patent References:
DE102004009704A1N/A
DE10356837A1N/A
Foreign References:
WO2011050938A1
WO2005044836A2
WO2006097320A2
WO2008043426A2
4711955
5047519
4828979
6211158
4804748
EP0286028
6579704
WO2001092284A1
WO1991006678A1
5798210
6255475
WO2001025247A1
6664079
5302509
WO2000050642A1
WO2004018493A1
WO2004018497A2
WO1999049082A2
WO2003048387A2
WO2002088382A2
WO1995031429A1
Other References:
"Nucleoside Triphosphates and their Analogs", Morteza Vaghefi, 2005, ISBN 1-57444-498-0
"Deoxynucleoside analogs in cancer therapy" Godefridus J. Peters, 2006, ISBN 1-58829-327-0
"Chemistry of nucleosides and nucleotides" Leroy B. Townsend, 1991, ISBN 0-306-43646-9
"Advanced organic chemistry of nucleic acids", 1994, Shabarova, ISBN 3-527-29021-4
"Nucleotide Analogs" Scheit, 1980, ISBN 0-471-04854-2
"Nucleoside and Nucleic Acid Chemistry", Kisakürek 2000
"Anti-HIV Nucleosides" Mitsuya, 1997
"Nucleoside Analogs in cancer therapy", Cheson, 1997
G. Wright et al. Pharmac. Ther. 1990, V. 47, S. 447-
D. Jameson et al. Methods in Enzymology 1997, V. 278, S. 363-
A. Draganescu et al. J. Biol. Chem. 2000 v. 275, 4555-
Burges et al. PNAS 1978 v. 75, S. 4798-
"Nucleoside triphosphates and their analogs", Morteza Vaghefi, 2005 ISBN 1-57444-498-0
Beabealashvilli et al. Biochem Biophys Acta 1986, v. 868, 136-, Yuzhanov et al. FEBS Lett. 1992 v. 306, 185-
A. Holy Current Pharmaceutical Design 2003 v. 9, 2567-, G. Wright et al. Pharmac. Ther. 1990, V. 47, S. 447-
"Nucleoside triphosphates and their analogs", Morteza Vaghefi, 2005 ISBN 1-57444-498-0
"Chemistry of Protein conjugation and crosslinking Shan S. Wong 1993 CRC Press Inc., Herman et al. Method in Enzymology 1990 V. 184 S. 584
Lomant et al. J. Mol. Biol. 1976 V. 104 243
"Chemistry of carboxylic acid and esters" S. Patai 1969 Interscience Publ., Pierce Katalog
"Protective groups in organic synthesis" 1991 John Wiley & Sons, Inc., V. Pillai Synthesis 1980 S. 1
V. Pillai Org. Photochem. 1987 V. 9 S. 225
"Neue photolabile Schutzgruppen für die lichtgesteuerte Oligonucleotidsynthese" H. Giegrich, 1996
"Neue photolabile Schutzgruppen für die lichtgesteuerte Oligonucleotidsynthese" S. M. Bühler, 1999, Konstanz
"Nucleoside triphosphates and their analogs", Morteza Vaghefi, 2005 ISBN 1-57444-498-0
"Chemistry of Protein conjugation and crosslinking" Shan S. Wong 1993
Y. Singh et al Chem. Soc. Rev. 2010, 39, 2054-
"Makromoleküle, Chemische Struktur und Synthesen", Band 1, 4, H. Elias, 1999, ISBN 3-527-29872-X
"Nucleoside triphosphates and their analogs", Morteza Vaghefi, 2005 ISBN 1-57444-498-0
"Chemistry of protein conjugation and crosslinking" Shan S. Wong 1993
"Bioconjugation: protein coupling techniques for the biomedical sciences", M. Aslam, 1996
Singh et al Chem Soc Rev, 2010, v. 39, 2054-
"Oligonucleotide synthesis, methods and applications" Piet Herdewijn, 2004, ISBN 1-58829-233-9
"Protocols for oligonucleotide conjugates, synthesis and analytical techniques" Sudhir Agrawal, 1993, ISBN 0-89603-252-3
"Protocols for oligonucleotide conjugates, synthesis and properties" Sudhir Agrawal, 1993, ISBN 0-89603-247-7
"The aptamer handbook" Sven Klussmann, 2006, ISBN 10: 3-527-31059-2
"Pharmaceutical aspects of oligonucleotides" Patrick Couvreur, 2000, ISBN 0-748-40841-X
"Triple Helix forming Oligonucleotides" Claude Malvy, 1999, ISBN 0-7923-8418-0
"Artificial DNA, methods and applications" Yury E. Khudyakov, ISBN 0-8493-1426-7
Bonnet et al. PNAS 1999, v 96. 6171-
"Bioconjugation: Protein coupling techniques for the biomedical sciences", M. Aslam, 1996, ISBN 0-333-58375-2
"Chemistry of Protein conjugation and crosslinking" Shan S. Wong 1993 CRC Press Inc.
"J Sambrook "Molecular Cloning" 3. Ed. CSHL Press 2001
"Molecular cloning" 1989, Ed. Maniatis, Cold Spring Harbor Laboratory
DNA-Replication" 1992. Ed. A. Kornberg, Freeman and company NY
Animal Cell DNA Polymerases" 1983, Fry M., CRC Press Inc., kommerziell erhältlich bei Chimerx
"Protocols for Oligonuccleotide and Analogs" S. Agrawal, 1993
"Protocols for Oligonucleotide conjugates" S. Agrawal 1994
Y. Singh et al Chem. Soc. Rev. 2010, 39, 2054-
www.Diercenet.com