|
Match
|
Document |
Document Title |
|
|
7615677 |
Assessment method
A method of assessing the viability of a cell comprises incubating the cell in a culture medium. The culture medium includes a plurality of amino acids and the change in concentration in the medium...
|
|
|
7612250 |
Nuclear transfer embryo formation method
A nuclear transfer embryo is formed by destabilizing microtubules of an oocyte, whereby essentially all endogenous chromatin collects at a second polar body during meiosis of an oocyte. The oocyte...
|
|
|
7601884 |
Method of producing cloned animals by demecolcine treatment
A cloned animal is produced by demecolcine treatment characterized by culturing the nuclear transfer embryo in vitro and transferring the embryo in vivo. This significantly improves in vitro...
|
|
|
7598082 |
Process of mammalian cell reprogramming through production of a heterokaryon
This invention relates to the reprogramming of animal cells and animal cell nuclei by nuclear addition. The invention also relates to the generation of animal cells, cell lines, tissues, organs,...
|
|
|
7592503 |
Mammalian telophase oocyte enucleation
The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an...
|
|
|
7547818 |
Metaphase donor cells for effective nuclear reprogramming in mammals
The present invention provides methods of producing a clone non-human mammalian nuclear transfer (NT) embryo and methods for producing a cloned non-human mammal. Embodiments of the methods include...
|
|
|
7547816 |
α(1,3)-galactosyltransferase knockout swine, tissues and organs
The invention relates to the genetic manipulation of non-human animals. More particularly, the invention relates to genetic manipulation of non-human animals to be used for xenotransplantation. The...
|
|
|
7531715 |
Double nuclear transfer method and results thereof
A method is described for the production of an animal (offspring) by the process of nuclear transfer. The animal may be produced using donor genetic material in cells taken directly from any stage...
|
|
|
7527974 |
Embryonic stem cells derived from human somatic cell—rabbit oocyte NT units
The present invention discloses a process for obtaining somatic cell derived embryonic stem cells (encoded by reprogrammed somatic cell nuclei), ES cell-like cells or other types of embryo-derived...
|
|
|
7524677 |
Mammalian cultured inner cell mass cell culture using a g1 cell as nuclear donor
A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at...
|
|
|
7446240 |
Method for the production of porcine nuclear transfer embryos
Methods for the production of nuclear transfer embryos, nuclear transfer embryos and animals derived therefrom are described. The method generally comprises at least the steps of: providing at...
|
|
|
7432415 |
Unactivated oocytes as cytoplast recipients for nonprimate mammalian and pig nuclear transfer
A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at...
|
|
|
7429690 |
Transgenic bovines having reduced prion protein production
The invention provides cloned transgenic ungulates (e.g., bovines) in which prion protein activity is reduced by one or more genetically engineered mutations. Desirably, these transgenic bovines...
|
|
|
7411111 |
Method for cloning of the rat by nuclear transfer
The invention relates to a method for cloning in the rat by nuclear transfer. The invention further relates to the rats obtained thus, in the foetal or adult state, as well as use thereof for the...
|
|
|
7371922 |
Nuclear transfer with porcine embryonic stem cells
Provided is a method for the production of transgenic animals, especially pigs, by the use of nuclear transfer from genetically modified or other embryonic stem cells to either enucleated oocytes...
|
|
|
7361804 |
Unactivated oocytes in nuclear transfer to produce ungulates
A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at...
|
|
|
7358416 |
Transgenic mouse expressing human FcRn protein
Disclosed is a transgenic knockout mouse whose genome comprises a homozygous disruption in its endogenous FcRn gene. The homozygous RcRn disruption prevents the expression of a functional FcRn...
|
|
|
7355094 |
Methods of ungulate nuclear transfer
A method of reconstituting an animal embryo involves transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit...
|
|
|
7351876 |
Efficient nuclear transfer with primordial gametes
This invention relates to a method for breeding animals via cloning and the animals obtainable by the method, in particular a method for reproducing animal embryos via efficient nuclear transfer...
|
|
|
7335811 |
Method for collecting cells in M phase or G1 phase and method for nuclear transplantation using the cells
Methods for collecting cells in M phase or G 1 phase, by which the percentage of M phase or G 1 phase cells is higher than that attained by the conventional methods are disclosed.
|
|
|
7332647 |
Fish produced by nuclear transfer from cultured cells
The disclosure provided herein teaches that fertile transgenic fish can be generated by nuclear transfer using cultured cells as embryonic fibroblasts.
|
|
|
7329796 |
Serial nuclear transfer of ungulate embryos
A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at...
|
|
|
7326824 |
Unactivated ungulate oocytes to produce a transgenic ungulate by nuclear transfer
A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at...
|
|
|
7323620 |
Activation of equine oocytes
A method for activating an equine oocyte comprising exposing oocyte to a medium containing a concentration of calcium of at least about 4 mM. Preferably the oocyte is exposed to this concentration...
|
|
|
7321076 |
Unactivated ungulate oocytes to produce a cloned ungulate by nuclear transfer
A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at...
|
|
|
7307198 |
Ungulates produced by nuclear transfer of G1 cells
A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at...
|
|
|
7294508 |
Isolation of inner cell mass for the establishment of human embryonic stem cell (hESC) lines
A method for isolating an inner cell mass comprising the steps of immobilizing a blastocyst stage embryo having a zona pellucida, trophectoderm, and inner cell mass, creating an aperture in the...
|
|
|
7291764 |
Cloning pigs using non-quiescent differentiated donor cells or nuclei
An improved method of nuclear transfer involving the transplantation of donor differentiated pig cell nuclei into enucleated pig oocytes is provided. The resultant nuclear transfer units are useful...
|
|
|
7265262 |
Telomerizing nuclear donor cells and improving the efficiency on nuclear transfer
This disclosure provides a system for creating cloned cells and embryos that are genetically modified. Cells are treated to increase expression of telomerase and potentially extend replicative...
|
|
|
7259291 |
Enucleation of avian eggs by gamma irradiation
A method of enucleating an egg by exposing the egg to gamma irradiation. The dose of gamma irradiation used is sufficiently high to halt development of the egg or embryo derived therefrom directed...
|
|
|
7253334 |
Methods for cloning non-human mammals using reprogrammed donor chromatin or donor cells
The invention provides methods for cloning mammals that allow the donor chromosomes or donor cells to be reprogrammed prior to insertion into an enucleated oocyte. The invention also features...
|
|
|
7235709 |
Transgenic animals with increased slow-twitch muscle fibers and methods of using such animals
Transgenic non-human mammals over-expressing MHCI in skeletal muscle are provided herein, as are methods of using these transgenic non-human mammals for screening candidate compounds for treating...
|
|
|
7232938 |
Cloning ungulates from a quiescent donor cell
A method of reconstituting an animal embryo involves transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit...
|
|
|
7202395 |
Assessment method
A method of assessing the viability of a cell comprises incubating the cell in a culture medium. The culture medium includes a plurality of amino acids and the change in concentration in the medium...
|
|
|
7196243 |
Transgenic animal containing CD200 and uses therefor
The present invention is directed toward a transgenic non-human animal and uses thereof, wherein each of the cells of the animal contain a transgene comprising a nucleic acid sequence encoding CD200.
|
|
|
7161058 |
Animal model for identifying agents that inhibit or enhance CTLA4 signaling
The present invention relates to a non-human transgenic animal, particularly a knock in mouse, whose genome comprises a heterologous, chimeric CTLA4 gene. The chimeric CTLA4 gene comprises exon 2...
|
|
|
7126039 |
Animal tissue with carbohydrate antigens compatible for human transplantation
This disclosure provides a system for generating animal tissue with carbohydrate antigens that are compatible for transplantation into human patients. The tissue is inactivated homozygously for...
|
|
|
7115795 |
Transgenic animals expressing salivary proteins
The invention provides a transgenic animal having within its genome a transgene construct for gastrointestinal tract specific expression of a protein. In a preferred embodiment, the protein is a...
|
|
|
7098376 |
Transgenic mouse expressing constitutively active hydrocarbon receptor
The present invention makes available powerful tools for the study of cancer, based on a novel expression construct for a constitutively active hydrocarbon receptor CA-AhR. The invention further...
|
|
|
7071373 |
Transgenic ungulate compositions and methods
Transgenic ungulates and compositions and methods for making and using same, are provided. Central to the invention are porcine, bovine, ovine and caprine embryonic stem cell lines and methods for...
|
|
|
7071372 |
Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells
An improved method of nuclear transfer employing long-term cultured somatic cells as the donor cells and enucleated oocytes as the recipient cells to produce dividing cybrids. Such cybrids are...
|
|
|
7064245 |
Process for reconstructing a non-human mammalian embryo by nuclear transfer using a heat treated donor nucleus and preparaing a non-human mammal
This invention relates to the generation of animals by the transfer of somatic cells which have been denatured preferably by heating, into enucleated metaphase II oocytes. This invention may be...
|
|
|
7053264 |
Nuclear reprogramming using IWSI and related chromatin remodeling ATPases
Methods and compositions for dedifferentiating nuclei from somatic cells are provided. Such methods and compositions are useful for facilitating processes such as, for example, cloning and...
|
|
|
7049480 |
Methods of enucleating an avian oocyte or zygote using two-photon laser scanning microscopy
The invention includes methods of enucleating avian eggs comprising visualizing internal structure of an avian egg utilizing TPLSM and ablating the nucleus of the egg by near-infrared light.
|
|
|
7041870 |
Transgenic transchromosomal rodents for making human antibodies
The present invention provides novel transgenic nonhuman mammals capable of producing human sequence antibodies, as well as methods of producing and using these antibodies.
|
|
|
7034200 |
Non-human transgenic animals expressing platelet-derived growth factor C (PDGF-C) and uses thereof
Non-human transgenic animals overexpressing PDGF-C and cells thereof have been created. The transgenic animals contain a nucleotide sequence that encodes for platelet derived growth factor C...
|
|
|
7030292 |
Method for producing a population of homozygous stem cells having a pre-selected immunotype and/or genotype, cells suitable for transplant derived therefrom, and materials and methods using same
A method of producing a homogenous population of homozygous stem (HS) cells pre-selected for immunotype and/or genotype from donor cells is described herein. The invention relates to methods of...
|
|
|
6906238 |
Effective nuclear reprogramming in mammals using CDK2 inhibitors
The present invention provides methods of producing a cloned non-human mammalian nuclear transfer (NT) embryo and methods for producing a cloned non-human mammal. Embodiments of the methods include...
|
|
|
6808704 |
Method for generating immune-compatible cells and tissues using nuclear transfer techniques
This invention relates to methods for making immune compatible tissues and cells for the purpose of transplantation and tissue engineering, using the techniques of nuclear transfer and cloning....
|
|
|
6781030 |
Methods for cloning mammals using telophase oocytes
The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an...
|