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5625055 |
Rapid isolation of polynucleotides
Polynucleotides (particularly DNA) formed from a lysate of cellular material in a holding tube are separated from proteins in a two-phase solvent system formed by a mild oxidising perchlorate and...
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5625054 |
DNA purification by solid phase extraction using fluorinated celite
The present invention relates to fluorinated surfaces which exhibit sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the...
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5625053 |
Method of isolating purified plasmid DNA using a nonionic detergent, solution
A method of isolating purified plasmid DNA includes the steps of providing cells containing plasmid DNA and lysing the cells with an alkyldimethylphosphine oxide (APO) detergent to produce a...
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5616700 |
Processes for synthesizing nucleotides and modified nucleotides using N.sub .
Disclosed herein are protecting groups for exocyclic amino groups of the base cytosine for use in the synthesis of oligonucleotides and oligonucleoside phosphorothioates, the protecting groups...
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5612181 |
Method for arranging a polynucleotide on a substrate for point-by-point analysis of the bases thereof
The invention relates to a method for arranging a polynucleotide on a substrate for point-by-point analysis of each successive base. It includes in combination anchoring the polynucleotide towards...
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5612473 |
Methods, kits and solutions for preparing sample material for nucleic acid amplification
The present invention provides methods and apparatus for detecting and discriminating multiple analytes within a test sample which are simple, user-friendly, cost-effective and fast. In particular,...
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5610074 |
Centrifugal method and apparatus for isolating a substance from a mixture of substances in a sample liquid
A centrifugal rotor effects the isolation, in a sequence of steps, of a substance from a mixture of substances dissolved, suspended or dispersed in a sample liquid. Multiple samples are processed...
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5610291 |
Glass fiber membranes modified by treatment with SiCl.sub.4, AlCl.sub.3 or BCl.sub.3 and washing with NaOH to set as a DNA Adsorbant
The present invention relates to modified glass fiber membranes which exhibit sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit...
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5605798 |
DNA diagnostic based on mass spectrometry
The invention provides fast and highly accurate mass spectrometer based processes for detecting a particular nucleic acid sequence in a biological sample. Depending on the sequence to be detected,...
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5604097 |
Methods for sorting polynucleotides using oligonucleotide tags
The invention provides a method of tracking, identifying, and/or sorting classes or subpopulations of molecules by the use of oligonucleotide tags. Oligonucleotide tags of the invention each...
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5596092 |
Extraction of genomic DNA from blood using cationic detergents
A process is disclosed for extracting genomic DNA from a biological sample containing a cell source comprising lysing the biological sample with a first cationic detergent in a solution of high...
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5591841 |
Rapid purification of circular DNA by triplex-mediated affinity capture
A single-step capture of a target supercoiled double-stranded DNA molecule is accomplished by forming a local triple-helix among two strands of the supercoiled circular DNA and an oligonucleotide...
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5589342 |
Kit for separating double-stranded nucleic acid from a single-stranded/double-stranded mixture of nucleic acids
A method and a kit for separating double-stranded nucleic acid molecules from a mixture containing both single-stranded and double-stranded nucleic acid molecules. The method is particularly...
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5587471 |
Method of making oligonucleotide libraries
The present invention provides methods of analyzing and evaluating phosphorus bearing monomeric units as to their suitability for use in preparing randomer oligomer libraries. The invention further...
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5582984 |
Methods of use of phenanthridium DNA intercalators for fluorescence detection
Phenanthridium compounds are provided which are comprised of intercalators and substituted intercalator segments having a functionalized chain or chains which provide a high affinity for binding to...
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5571669 |
Transcription and nucleic acid sequence determination with short primer DNA/RNA molecules and RNA polymerase
In vitro transcription of RNA or DNA templates from short RNA or DNA primers is acheived in the absence of a DNA promoter sequence. The progressive nature of the elongation complex according to the...
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5559221 |
Method of separating oligonucleotides from a mixture
A method of purifying full length synthetic target oligonucleotides from a mixture of oligonucleotides, particularly from a mixture containing truncated or failed sequences. The method involves...
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5543305 |
Methods of extracting deoxyribonucleic acids without using a proteolytic enzyme
This invention provides a rapid and highly effective method for extracting nucleic acids from cells or virions without the use of proteolytic enzymes. Extraction is accomplished within a few...
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5534054 |
Silicon tetrahydrazide for purification of DNA
Silicon tetrahydrazide compounds produced by reacting SiCl 4 with hydrazine to completion. The compounds are useful for binding DNA, as they do not require the use of chaotrope or alcohol...
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5527673 |
Apparatus and method for transport of nucleic acid sequences by capillary action on a solid support and detection of the nucleic acid sequences
Apparatus for transport of molecules including nucleic acid sequences in a bibulous carrier comprising a dry bibulous carrier defining a capillary transport path which supports the transport of the...
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5523392 |
Purification of DNA on aluminum silicates, and phosphosilicates
A process for purifying DNA comprising binding the DNA to a silicate compound in the presence of water, low salt buffer, or physiological buffer and eluting in the bound DNA by heating in a low...
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5506103 |
Method for detecting charged oligonucleotides in biological fluids
Disclosed is a method for detecting and quantitating oligonucleotides with charged internucleotide linkages in biological fluids. In this method, a biological fluid sample is contacted with an...
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5506348 |
Matrix for matrix-assisted laser desorption mass spectroscopy
The invention relates to a composition comprising (a) at least one ammonium salt of an inorganic or organic acid, (b) a compound of formula I ##STR1## wherein R is an aliphatic hydrocarbon...
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5503816 |
Silicate compounds for DNA purification
The present invention relates to a silicon-containing material which exhibits sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit...
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5500342 |
Method for determining sugar chain structure
A more exact, reliable, and simple method for determining the structure of a sugar chain, a kit to be used for this method, and novel oligosaccharides useful for the method as standard sugar chains...
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5496473 |
Apparatus for processing biopolymer-containing columns
An apparatus has been designed that is useful for the automated or semi-automated removal, recovery, deprotection, and/or purification of biopolymers such as oligodeoxyribonucleotides,...
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5491224 |
Direct label transaminated DNA probe compositions for chromosome identification and methods for their manufacture
Direct label probe compositions which stain DNA of a preselected single chromosome or region of a chromosome of a multi-chromosomal genome are provided that comprise mixed DNA segments which are...
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5491133 |
Methods for blocking the expression of specifically targeted genes
The use of oligodeoxynucleotides modified at the 3'-terminal internucleotide link as therapeutic agents by a method of hybridizing the modified oligonucleotide to a complementary sequence within a...
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5459253 |
M-RNA purification
A method kit for purification of m-RNA from a cell are disclosed. Guanidine containing moieties, at high molarity, are use to quickly lyse the cell. They also act to inhibit RNase activity. Without...
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5455344 |
Agarose compositions for nucleic acid sequencing
A nucleic acid sequencing gel comprising 2 to 10% of a gelling polysaccharide, preferably agarose; a denaturing agent, a non-gelling additive, preferably glycerol, and electrophoretic buffers. This...
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5440025 |
Process for separating nucleic acid polymers
A method is disclosed for separating a nucleic acid polymer without substantially denaturing the nucleic acid polymer. The method includes contacting a liquid medium, in which the nucleic acid...
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5438128 |
Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
The present invention relates to a device method and kit for the convenient and rapid isolation and purification of nucleic acids, proteins, peptides, carbohydrates and oligosaccharides from...
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5437976 |
Multi-domain DNA ligands bound to a solid matrix for protein and nucleic acid affinity chromatography and processing of solid-phase DNA
The present invention is directed to a multi-domain oligonucleotide ligand having multiple functions conferred by each of the domains. The ligand is bound to a solid phase matrix for use in both...
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5434049 |
Separation of polynucleotides using supports having a plurality of electrode-containing cells
A method for simultaneously detecting a plurality of target polynucleotides in a sample on a single reaction chip and a method for separating a plurality of target polynucleotides are provided. On...
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5422241 |
Methods for the recovery of nucleic acids from reaction mixtures
A method for the recovery of nucleic acids from a reaction mixture, such as nuclease protection assays, comprising the use of one reagent containing a) nuclease for digesting single-stranded RNA...
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5420265 |
Separation of phosphorothioate oligonucleotides by capillary gel electrophoresis
A method for separating a mixture of 5'-phosphorothioate mononucleotides, a mixture of 5'-phosphorothioate oligonucleotides, or a mixture of both 5'-phosphorothioate mononucleotides and...
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5418284 |
Surface-modified polyacrylonitrile beads
A bead composition is disclosed herein which comprises a core of a polyacrylonitrile homopolymer or copolymer and a surface of pendant N-haloamide groups. Also disclosed is a process for the...
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5405951 |
Solid phase extraction purification of DNA
The invention provides a method for purifying DNA from any source in any form. The method comprises the use of water soluble organic solvents when purifying DNA. By using water soluble organic...
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5395928 |
Chromatographic separation of phosphorothioate oligonucleotides
A method is provided for the preparation and/or analysis of synthetic phosphorothioate and -dithioate oligonucleotides. In particular, the method permits separation of fully sulfurized...
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5386024 |
Method to prepare nucleic acids from a biological sample using low pH and acid protease
Method for making available a desired nucleic acid contained in a biological sample, comprising the steps of acidifying said biological sample to a pH at which endogenous nucleases capable of...
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5380833 |
Polynucleotide reagents containing selectable cleavage sites
Novel methods for assaying a nucleic acid analyte are provided, which employ polynucleotides having oligonucleotide sequences substantially homologous to a sequence of interest in the analyte,...
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5380645 |
Generalized method for assessment of colorectal carcinoma
A method is provided for assessing the generalized genetic change which occurs during tumorigenesis. The method relies on measurement of loss of a large number of alleles from the chromosomes of...
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5374522 |
Method for releasing RNA and DNA from cells
A method is disclosed for disrupting cells, including microorganisms, and facilitating thereby the release of cellular components including RNA and DNA into solution. Solutions or suspensions of...
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5352777 |
DNA isolation from animal cells
A novel method for the isolation of high molecular weight DNA from plants, yeast bacteria, and animal cells or tissue employs xanthate forming compounds, such as sodium/potassium ethyl...
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5352609 |
Cartridge, apparatus, and method for preparing purified nucleic acids from a cell sample
A cartridge for preparing nucleic acids such as DNA from cells comprises two end rings (14) enabling it to be mounted in sealed manner in a tube (10), two dialysis membranes (16) delimiting a...
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5346994 |
Shelf-stable product and process for isolating RNA, DNA and proteins
Shelf-stable solvent solutions and methods for simultaneously isolating RNA, DNA and proteins from biological samples are disclosed. The solvent solutions include phenol and a guanidinium compound,...
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5342931 |
Process for purifying DNA on hydrated silica
A process for purifying DNA comprising 1) binding the DNA to a hydrated silica in the presence of water or physiological buffers in which the hydrated silica is prepared by refluxing silicon...
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5330914 |
Apparatus and method for automated purification of extra-chromo-somal DNA from a cell suspension in a container
This invention relates to an apparatus for automatic purification of extra-chromosomal DNA from a cell suspension in a container. The apparatus comprises a device for introducing the cell...
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5329000 |
Purification of DNA with silicon tetrahydrazide
A process for purifying DNA in which the DNA is bound to silicon tetrahydrazide in the presence of less than 2M chaotrope, low salt buffers, or water. The DNA is then eluted with low salt buffer or...
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5324829 |
High specific activity nucleic acid probes having target recognition and signal generating moieties
The probes of the invention are made up of a target recognition moiety and a signal generation moiety. In the first step of the preparatory method of the invention, the 5' or 3' terminus or both of...
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