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5990301 |
Process for the separation and purification of nucleic acids from biological sources
A process for the isolation and purification of nucleic acids and/or oligonucleotides for use in gene therapy wherein nucleic acids and/or oligonucleotides are isolated or purified from an...
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5986085 |
Matched ion polynucleotide chromatography (MIPC) process for separation of polynucleotide fragments
A batch process for obtaining polynucleotide fragments, such as dsDNA, having a selected size from a mixture of polynucleotide fragments including the steps of a) applying a solution of the mixture...
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5981185 |
Oligonucleotide repeat arrays
A solid support based hybridization assay is provided which allows for the systematic and reproducible analysis of repeat and tandem repeat oligonucleotide sequences of DNA and RNA by hybridization...
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5981736 |
One step device and process for concentration and purification of biological molecules
A device for one step purification of a desired biological molecule from a sample, wherein the device comprises a housing loaded with an adsorptive media of a known volume on top of a size...
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5973137 |
Low pH RNA isolation reagents, method, and kit
The present invention describes an RNA isolation process which utilizes low pH reagents. In addition, the reagents are less hazardous and are more stable than those used in prior art methods. This...
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5969129 |
Purification of polynucleotides from polynucleotide/polysaccharide mixtures
A process for isolating polynucleotides from aqueous mixtures containing both polynucleotides and polysaccharides is described. The process uses polymer gels containing --B(OH) 2 groups, and is...
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5962666 |
Deformylase
The invention provides def polypeptides and DNA (RNA) encoding def polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing def...
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5945515 |
Product and process for isolating DNA, RNA and proteins
Solutions and methods are disclosed for the effective, simple isolation/extraction of DNA, RNA and proteins from a single biological material sample, such as cells, tissues and biological fluids....
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5945520 |
Chromatographic resins and methods for using same
Disclosed are rationally designed mixed mode resins which are useful in recovering a target compound from an aqueous solution and methods for use of such resins. The resins described herein have a...
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5942610 |
Non-nucleoside 1,3-diol reagents and their use to label or modify synthetic oligonucleotides
The subject invention concerns novel reagents and methods useful for introducing functional groups and labels at multiple sites of a nucleotide. The reagents are useful, for example, in...
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5932717 |
Methods of obtaining DNA-RNA hybrids from sturgeon milt
The present invention pertains to DNA-RNA hybrids endowed with immunotropic and antiviral activity, a pharmaceutical preparation made on the basis thereof, and the process for the preparation of...
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5916776 |
Amplification method for a polynucleotide
The nucleic acid amplification procedures of the present invention provide methods for amplification of a nucleic acid comprised of a first strand, comprising (a) using the first strand to generate...
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5916744 |
Testing for infestation of rapeseed and other cruciferae by the fungus Leptosphaeria maculans (blackleg infestation)
A method of testing for infestation of tissue of rape or other Cruciferea with a virulent strain of Leptosphaeria maculans. The method involves isolating DNA of L. maculans from the tissue;...
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5916775 |
Method for the purification of DNA
Disclosed is a method for collecting DNA by lysing microbial cells, adsorbing released DNA on a carrier and collecting the DNA adsorbed on the carrier, which method comprises the following steps of...
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5912332 |
Affinity-based purification of oligonucleotides using soluble multimeric oligonucleotides
The present invention provides novel compounds and methods for purifying oligonucleotides. The compounds according to the invention are multimeric oligonucleotides comprising a multimerization...
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5910408 |
Catalytic DNA having ligase activity
The invention features nucleic acid molecules and, in particular, DNA molecules having catalytic activity, as well as methods for obtaining and using such nucleic acid molecules.
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5910584 |
Method for isolating plasmid DNA
Method for isolating plasmid DNA, which comprises the steps of: suspending hydroxyapatite particles in a tris-EDTA buffer solution having a pH of about 7 to about 10, about 10 to about 50 mM of an...
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5908845 |
Polyether nucleic acids
A compound comprising a polyether backbone bearing a plurality of ligands that are individually bound to chiral carbon atoms located within said backbone, at least one of said ligands including a...
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5898071 |
DNA purification and isolation using magnetic particles
A method of separating polynucleotides, such as DNA, RNA and PNA, from a solution containing polynucleotides by reversibly and non-specifically binding the polynucleotides to a solid surface, such...
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5892007 |
Method and means for oligonucleotide separation
A protecting group for use in separating oligodeoxyribonucleotide compounds having the formula Ar--L--Y, wherein Ar represents a substantially planar, fused ring system containing at least fused...
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5876941 |
Detection of mismatches by resolvase cleavage on a solid support
Disclosed is a method for detecting one or more mismatches in a test nucleic acid which preferentially hybridizes to a reference nucleic acid. The method involves a) providing the test nucleic acid...
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5869641 |
High affinity nucleic acid ligands of CD4
Methods are described for the identification and preparation of high-affinity nucleic acid ligands to CD4. Included in the invention are specific 2'F RNA ligands to CD4 identified by the SELEX method.
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5869696 |
Universal solid supports and methods for their use
Universal solid support oligonucleotide synthesis reagents, oligonucleotide synthesis processes, and reagents for cleaving oligonucleotides from solid supports are disclosed. Oligonucleotide...
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5866326 |
Method for isolating vegetative insecticidal protein genes
The present invention is drawn to pesticidal strains and proteins. Bacillus strains which are capable of producing pesticidal proteins and auxiliary proteins during vegetative growth are provided....
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5866429 |
Precision and accuracy of anion-exchange separation of nucleic acids
Solvents for salt-gradient anion-exchange separation of nucleic acids, especially double-stranded DNA and especially by liquid chromatography, are improved by replacing NaCl as the eluting salt...
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5861254 |
Flow cell SELEX
Described herein are methods for improved partitioning between high and low affinity nucleic acid ligands identified through the SELEX method, termed Flow Cell SELEX. The Flow Cell SELEX method...
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5856465 |
Compositions and methods for the synthesis of chirally pure organophosphorus nucleoside derivatives
Methods for the stereospecific synthesis of chirally pure organophosphorus dinucleotide derivatives and nucleoside monomer synthons used in their synthesis are provided. These methods allow for...
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5856464 |
Selective capping solution phase oligonucleotide synthesis
Improved methods for solution-phase synthesis of purified short-chain oligonucleotide coupling units, such as dimers, trimers, and tetramers, suitable for use in solution-phase or solid-phase...
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5856100 |
Method for purification and transfer to separation/detection systems of DNA sequencing samples and plates used therefor
Disclosed are: a part for assembling reaction vessels comprising a base plate with holes passing through said plate in the direction of thickness; a method for preparation of reaction vessels...
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5851805 |
Method for producing DNA from mRNA
An improved method for amplifying cDNA fragments corresponding to the mRNA species expressed in cells is described. The method involves priming the mRNA with a first labeled synthetic...
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5849479 |
High-affinity oligonucleotide ligands to vascular endothelial growth factor (VEGF)
Methods are described for the identification and preparation of nucleic acid ligand ligands to vascular endothelial growth factor (VEGF). Included in the invention are specific RNA ligands to VEGF...
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5846721 |
Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs
This invention provides a method to normalize a cDNA library comprising: (a) constructing a directionally cloned library containing cDNA inserts wherein the insert is capable of being amplified by...
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5846832 |
Apparatus and method for shear breakage of polynucleotides
Apparatus and method for producing a defined size distribution of polynucleotide fragments having a selected mean size range between about 100 and 20,000 base pairs. The method includes passing a...
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5846716 |
Method for nucleotide sequence amplification
This invention relates to an improvement of the procedure for amplifying a target nucleotide sequence by using an effective amount of a glycine-based osmolyte in the reaction mixture of an...
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5846713 |
High affinity HKGF nucleic acid ligands and inhibitors
Methods are described for the identification and preparation of high-affinity nucleic acid ligands to hKGF. Included in the invention are specific RNA ligands to hKGF identified by the SELEX...
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5846783 |
Methods and apparatus for preparing, amplifying, and discriminating multiple analytes
The present invention provides methods and apparatus for detecting and discriminating multiple analytes within a test sample which are simple, user-friendly, cost-effective and fast. In particular,...
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5843653 |
Method for detecting a target molecule in a sample using a nucleic acid ligand
This invention discloses a method of detecting the presence or absence of a target molecule in a sample and a method of measuring the amount of a target molecule in a sample using nucleic acid...
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5843312 |
Chromatography material
A chromatography material is described for separation of nucleic acid mixtures in which a support is converted with a silanization reagent, in which the silanization reagent has a reactive group...
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5840862 |
Process for aligning, adhering and stretching nucleic acid strands on a support surface by passage through a meniscus
Macromolecules such as nucleic acid strands are aligned, adhered and stretched on a support surface by passing the strands through a meniscus of a solvent containing the strands. The meniscus may...
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5840877 |
Electrophoresis gels of enhanced selectivity
Electrophoresis gels of enhanced selectivity are produced by adding a preformed polymer to a polymerization solution containing at least one monomer and at least one cross-linker. The polymer must...
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5837524 |
PYK2 related polynucleotide products
The present invention features a method for treatment of an organism having a disease or condition characterized by an abnormality in a signal transduction pathway, wherein the signal transduction...
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5830662 |
Method for construction of normalized cDNA libraries
This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional...
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5830643 |
Partially double-stranded oligonucleotide and method for forming oligonucleotide
A method for forming an oligonucleotide comprises the steps of synthesizing a pair of oligonucleotides, one oligonucleotide having a base sequence portion comlementary to that of the other at a...
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5831070 |
Printing oligonucleotide arrays using deprotection agents solely in the vapor phase
A method and apparatus for selectively applying a print material onto a substrate for the synthesis of an array of oligonucleotides at selected regions of a substrate. The print material includes a...
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5824787 |
Polynucleotide sizing reagent
A polynucleotide marker reagent is described for use in DNA and RNA size determination.
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5821058 |
Automated DNA sequencing technique
A process for the electrophoretic analysis of DNA fragments produced in DNA sequencing operations wherein chromophores or fluorophores are used to tag the DNA fragments produced by the sequencing...
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5817798 |
Rapid RNA isolation procedure in the presence of a transition metal ion
Provided herein is a rapid method for isolating total RNA from test samples and further for isolating mRNA from test samples.
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5817797 |
Sequencing DNA; a modification of the polymerase chain reaction
The invention provides a process wherein a biotinylated oligonucleotide primer and an oligonucleotide primer which has not undergone biotinylation are used when amplifying a DNA sequence to...
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5811538 |
Process for the purification of oligomers
Methods for purifying an oligomer by normal phase column chromatography on a support selected from polyhydroxyethyl aspartamide, hydrophilic silica and silica from an oligomer impurity having a...
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5808041 |
Nucleic acid purification using silica gel and glass particles
The present invention provides compositions and methods for isolating nucleic acids with lengths greater than about 50 bases, from cells, gels, solutions and other media, in which nucleic acids...
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