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7074596 |
Synthesis and use of anti-reverse mRNA cap analogues
The ability to synthesize capped RNA transcripts in vitro has been of considerable value in a variety of applications. However, one-third to one-half of the caps have, until now, been incorporated...
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7074333 |
Recovery of linear nucleic acids by salt dilution and/or reduced pressure prior to continuous pressure differential ultrafiltration
A method of recovery of DNA fragments using ultrafiltration. The method of the present invention relates to the separation and/or fractionation of double stranded linear nucleic acids using...
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7074917 |
Method for purifying 5′ -protected 2′ -deoxypurine nucleosides
A method for efficiently purifying 5′ protected 2′-deoxypurine nucleosides, efficient production of which has previously been difficult. Impurities can be separated by obtaining the 5′...
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7067287 |
Method for recovery of nucleic acids
Methods for the recovery of nucleic acids from a nucleic acid-containing material are provided, by which nucleic acids can be rapidly and easily recovered at a high purity without deteriorating the...
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7041817 |
Synthesis, deprotection, analysis and purification of RNA and ribozymes
Method for purification and synthesis of RNA molecules and enzymatic RNA molecules in enzymatically active form.
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7037654 |
Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents
Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a...
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7030230 |
Process of purifying phosphoramidites
A process of purifying phosphoramidite precursors useful in inter alia synthesis of oligonucleotides comprises dissolving a crude phosphoramidite in a polar phase, adding a basic compound to the...
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7026468 |
Process and equipment for plasmid purification
A scalable alkaline lysis process, including procedures and devices for the isolation of large quantities (grams and kilograms) of plasmid DNA from recombinant E. coli cell. Effective,...
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7022835 |
Method for binding nucleic acids to a solid phase
The invention concerns a method for binding nucleic acids to a solid phase in which a nucleic acid containing solution is contacted with a solid phase which has hydrophobic and hydrophilic groups...
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7018538 |
Use of a composite polymer-coated sorbent for separation, purification, desalting and concentration of biopolymers
Use of a composite sorbent having an at least partial coating on a support, which coating comprises essentially polyanilines or derivatives of polyanilines for the separation, isolation,...
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6992181 |
DNA isolation method
Disclosed is a method and apparatus for the isolation of DNA or cell nuclei or a mixture thereof from cell samples in a CD device. The method includes treating a suspension of whole cells with a...
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6992182 |
Method for isolating DNA from biological materials
The invention relates to a method for the stabilization, purification or/and isolation of nucleic acids from biological materials, in particular stool samples which may contain contaminations and...
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6972329 |
Materials and methods for releasing genetic material
The invention provides methods for isolating and releasing genetic material from a solid medium, such as a DNA array, a filter, a card, or a multiple-well plate, using a wash solution comprising a...
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6969587 |
Detection of nucleic acid heteroduplex molecules by anion-exchange chromatography
The present invention describes a method for separating or partially separating heteroduplex and homoduplex DNA molecules in a mixture. In the method, the mixture is applied to an anion-exchange...
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6969603 |
Method for isolating DNA
Method for isolating DNA contained in a biological sample. The method includes combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier,...
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6962784 |
Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes
This invention discloses a method for preparing a complex comprised of a VEGF Nucleic Acid Ligand and a Non-Immunogenic, High Molecular Weight Compound or Lipophilic Compound by identifying a VEGF...
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6958392 |
Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products
A method for isolating nucleic acid which comprises:
(a) applying a sample comprising cells containing nucleic acid to a filter, whereby the cells are retained as a retentate and...
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6958391 |
Method for purifying 5′-protected 2′-deoxypurine nucleosides
A method for efficiently purifying 5′ protected 2′-deoxypurine nucleosides, efficient production of which has previously been difficult. Impurities can be separated by obtaining the 5′...
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6936414 |
Nucleic acid isolation method and kit
Provided herein is a method for separating nucleic acid from a test sample comprising the steps of contacting a test sample with a metal oxide support material and a binding buffer to form nucleic...
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6936709 |
Method for purifying protected 2′-deoxycytidines and hydrated crystals thereof
A protected 2′-deoxycytidine is purified by precipitating the protected 2′-deoxycytidine represented by general formula (3) in the form of a hydrated crystal from a solution containing the...
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6933379 |
Method for linking nucleic acids and/or glycosaminoglycans to polar/hydrophilic materials
The invention relates to a method for linking nucleic acid and/or glycosaminoglycan or glycosaminoglycan mimetics to a polar/hydrophilic material, characterized by contacting a nucleic acid and/or...
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6933116 |
Nucleic acid ligand binding site identification
This invention comprises nucleic acid ligand for use as a diagnostic reagent for detecting the presence or absence of a target molecule in a sample, and a diagnostic reagent to measure the amount...
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6933114 |
Nucleic acid ligands to the prostate specific membrane antigen
Methods are provided for generating nucleic acid ligands of Prostate Specific Membrane Antigen (PSMA). The methods of the invention use the SELEX method for the isolation of nucleic acid ligands....
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6926823 |
Polymer with superior polar retention for sample pretreatment
A polymeric sorbent that can be employed in the extraction and purification of polar and nonpolar molecules from a complex media (e.g. pharmaceuticals from biological matrices) by solid phase...
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6921817 |
Methods for simultaneous isolation of biologically active transcription factors and DNA
A rapid isolation method permits the isolation of DNAs, RNAs and transcription factors simultaneously from a wide range of lymphoid and non-lymphoid cells, either from patients or in culture,...
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6919444 |
Magnetic particles for purifying nucleic acids
Preparations of particles having a glass surface, wherein more than 75% by weight of these particles have a particle size between 0.5 μm and 15 μm and a glass surface which contains between 2 and...
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6916919 |
Isolation of DNA molecules
The present invention relates to a process for separating nucleic acid molecules, preferably open circular and supercoiled plasmid DNA and RNA molecules from each other, comprising the steps of...
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6914137 |
Isolation of nucleic acids
A method for extracting nucleic acids from a biological material such as blood comprises contacting the mixture with a material at a pH such that the material is positively charged and will bind...
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6881541 |
Method for analyzing a fluid sample
A method for extracting nucleic acid from a fluid sample comprises the steps of introducing the sample into a cartridge having a sample flow path and a lysing chamber in the sample flow path. The...
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6875857 |
Reagent for the isolation of RNA
The present invention provides RNA extraction reagents, methods and kits that are especially useful for extracting RNA. The reagents, methods and kits of the present invention are especially useful...
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6872818 |
Ammonium sulfate for neutralization of inhibitory effects
The present invention provides RNA purification and/or analysis methods that use ammonium sulfate to mitigate or neutralize inhibitory effects of certain molecules. Exemplary inhibitory molecules...
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6870047 |
Magnetic pigment
Magnetic particles are prepared containing a magnetic core coated with a glass layer having a substantially pore-free class surface or having pores with a diameter offices than 10 nm. The particles...
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6858722 |
Oligomer phosphoramidite compositions and processes for synthesizing the same
Synthetic processes are provided wherein oligomers are prepared using phosphoramidite compositions. Oligomers having phosphodiester, phosphorothioate, phosphorodithioate covalent linkages are...
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6855816 |
Method for producing endotoxin-free nucleic acids and the use thereof
Method for isolating and purifying nucleic acids and/or oligonucleotides from a biological sample, the use of the isolated or purified nucleic acid and/or oligonucleotide for transfecting cells and...
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6844151 |
Methods for production of arrays with modified oligonucleotide and polynucleotide compositions
The present invention provides methods for producing arrays having associated modified nucleic acid structures, e.g. acid stable and/or end-blocked nucleic acids such as 2′-O—R...
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6828097 |
Single copy genomic hybridization probes and method of generating same
Nucleic acid (e.g., DNA) hybridization probes are described which comprise a labeled, single copy nucleic acid which hybridizes to a deduced single copy sequence interval in target nucleic acid of...
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6828436 |
Method for selecting a high affinity, low molecular weight displacer for oligonucleotide purification
A method for purifying oligonucleotides by displacement chromatography on anion-exchange media, using high affinity, low molecular weight (less than about 10000 Da) displacers, is disclosed....
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6825340 |
Methods and reagents for inactivating ribonucleases
The present invention is a general method for inactivating or inhibiting ribonucleases. Ribonucleases are treated with a reducing agent and heat. RNA samples contaminated with ribonuclease may be...
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6821757 |
Rapid and simple process for isolation of circular nucleic acids
A method for separating and/or isolating circular nucleic acids from a mixture having different species of nucleic acids other than circular nucleic acids wherein the mixture is treated under...
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6818760 |
Removal of dye-labeled dideoxy terminators from DNA sequencing reactions
This invention provides methods for removing unincorporated dye-labeled molecules from a mixture that includes dye-labeled polynucleotides or other polymers and the unincorporated dye-labeled...
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6815541 |
Coprecipitant and method for extracting nucleic acids
A coprecipitant and a nucleic-acid extraction method using the coprecipitant are provided. The coprecipitant has affinity to the nucleic acids, no competitive inhibition to the reverse...
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6815167 |
Amplification of DNA to produce single-stranded product of defined sequence and length
The present disclosure relates to methods for generating single-stranded DNA molecules of defined sequence and length. Specifically, a region of template containing target sequence is amplified by...
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6812341 |
High efficiency mRNA isolation methods and compositions
The present invention provides methods and compositions, including kits, for the isolation and purification of mRNA, particularly poly(A) RNA. It concerns the use of isostabilizing salts such as...
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6806362 |
pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
Disclosed are pH dependent ion exchange matrices, methods of making, and methods of use.
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6800752 |
Chromatography material and a method using the same
A chromatography material for separation of nucleic acid mixtures, having a carrier and ion exchanger functions applied to it is described, whereby the carrier comprises a fibrous material. In...
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6787310 |
Strand displacement methods employing competitor oligonucleotides for isolating one strand of a double-stranded nucleic acid
The present invention provides methods and kits for isolating one strand of a double-stranded target nucleic acid. The method capitalizes on the differences in the kinetics and thermodynamic...
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6777181 |
Method for separating and collecting nucleic acids
A method for separating and collecting nucleic acids, which comprises: a step of bringing a sample nucleic acid solution into contact with a nucleic acid-immobilized substrate comprising a...
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6773913 |
Device and process for purifying vectors
In a vector purification device, bacteria containing vectors are cultured in a culture medium in a culture tank, the culture medium is concentrated by filtration with a first TFF film, and the...
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6764822 |
DNA typing by mass spectrometry with polymorphic DNA repeat markers
The present invention is related to the fields of genetic mapping and genetic identity detection, including forensic identification and paternity testing. This invention is more specifically...
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6764859 |
Device and method for mixing magnetic particles with a fluid
This invention relates to the use of magnetic or magnetizable particles, and, in particular, to methods of mixing magnetic or (super)paramagnetic particles efficiently with a fluid and the...
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