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5319079 |
Process for terminal substituting of a polynucleotide
Method for substituting a substitution compound for the hydrogen of a support bound primary hydroxyl group. The support bound primary hydroxyl group is treated in an organic, substantially...
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5316925 |
T-cell receptor specific for antigen polypeptides and related polynucleotides
Oligonucleotide sequences are provided coding for T-cell-specific antigen receptors or fragments thereof. The oligonucleotide sequences can be used as probes for detecting helper and cytotoxic...
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5310894 |
Solid phase polynucleotide syntheses
Methods for solid-state synthesis of polymers, especially polypeptides and polynucleotides, are provided. In accordance with a preferred embodiment, selectively activatable and reactive bonding...
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5292639 |
Association of bovine mitochondrial DNA with traits of economic importance
A method of genetically evaluating animals by using mitochondrial DNA is disclosed. Polymorphisms in mitochondrial DNA are detected by isolating, fragmenting, and sequencing the DNA. The...
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5284911 |
Surface-modified polyacrylonitrile substrates
A composition is disclosed herein which comprises a core of a polyacrylonitrile homopolymer or copolymer and a surface of N-haloamides. Also disclosed is a process for the production of said...
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5284940 |
Preparation for nucleic acid samples
Methods and compounds for the preparation of nucleic acid samples. Polymerase inhibitors are treated so as to allow for amplification of nucleic acid from biological sources, including whole blood.
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5279938 |
Sensitive diagnostic test for lyme disease
The nucleotide sequence of a recombinant clone containing a specific segment of Borrelia burgdorferi DNA which enables the identification of the spirochetes causing Lyme disease has been provided....
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5274141 |
Designed quinone- and hydroquinone-containing cyclic enediyneols and enediyneones having DNA cleaving and cytotoxic properties
Quinone- and hydroquinone-containing cyclic enediyneols and enediyneones having 10-carbon atoms in the enediyne-containing ring that cleave DNA are disclosed, as are methods of making and using the...
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5260429 |
Purified DNA of the 170-kd surface Gal/GalNAc adherence lectin of pathogenic E. histolytica
cDNA encoding the 170 kd subunit of Gal/GalNAc lectin in various strains of E. histolytica is provided. Availability of this cDNA permits the use of diagnostic assays to distinguish pathogenic from...
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5252724 |
Method of extracting particular nucleic acid fragment
A method of extracting a particular nucleic acid fragment containing a nucleic acid sequence of interest from a nucleic acid or nucleic acids mixture, comprising the steps of: (1) digesting the...
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5234809 |
Process for isolating nucleic acid
The invention relates to a process, a combination of means for isolating nucleic acid from a nucleic acid-containing starting material and a testkit in order to amplify the nucleic acid obtained by...
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5155018 |
Process and kit for isolating and purifying RNA from biological sources
A process is provided for isolating and purifying biologically active RNA from a biological sources containing RNA, DNA and other cellular materials. The process involves contacting the...
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5149408 |
Capillary blotting pad for molecular transfer to membranes
A capillary blotting sandwich for transferring soluble macromolecules in a liquid medium from a liquid-permeable matrix to a semi-permeable receiving membrane. The blotting sandwich includes: (i) a...
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5141813 |
Multifunctional controlled pore glass reagent for solid phase oligonucleotide synthesis
The subject invention concerns a novel multifunctional solid support reagent which is useful in solid phase oligonucleotide synthesis. Specifically, the reagent is used in a solid phase...
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5132207 |
Accelerated nucleic acid reassociation method
A method for the formation of double stranded nucleic acid molecules from separate single stranded nucleic acid molecules in a single phase reaction solution is disclosed wherein the rate of...
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5124247 |
Process for sequencing nucleic acids
For the sequencing of nucleic acids by enzymatic extension of an oligonucleotide primer in the presence of a polymerase, the four deoxyribonucleoside triphosphates, one of the four...
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5112957 |
Method and apparatus for washing solid media
A method is described for safely and effectively washing solid gels or films containing biological macromolecules such as proteins or nucleic acids. The method involves placing the gels in an open...
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5112459 |
Apparatus and method for transfer of macromolecules
An apparatus and method for the transfer of macromolecules from a source medium to a transfer medium employ positive pressure to urge a sample from the source medium to the transfer medium. The...
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5112461 |
Geltrans, a device for transfer of gel to paper
A support frame with multiple water jets, together with a support grid and carrier frame, is described. Methods for their construction from commonly available components, and methods for their use...
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5106966 |
Use of gel polymer for DNA extraction with organic solvents
The invention describes a method for extracting DNA from a sample. It involves contacting a sample with a separation reagent which permits differentiated solvation of DNA and protein. By adding a...
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5063162 |
Process for isolating nucleic acids utilizing protease digestion
A process for the isolation of nucleic acids from cell or tissue extracts by precipitating the nucleic acids with a water-soluble ketone, preferably with acetone, optionally in the presence of...
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5057426 |
Method for separating long-chain nucleic acids
A method for the separation of long-chain nucleic acids from other substances in solutions containing nucleic acids and other materials, comprising fixing long-chain nucleic acids in a nucleic...
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5010183 |
Process for purifying DNA and RNA using cationic detergents
A novel method is provided for purifying DNA and RNA from a variety of sources, including cells, cell lysates, viruses, tissues, blood and other body fluids employing a cationic detergent to...
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5003059 |
Determining DNA sequences by mass spectrometry
This invention relates to the methods, apparatus, reagents and mixtures of reagents for sequencing natural or recombinant DNA and other polynucleotides. In particular, this invention relates to a...
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5002868 |
DNA sequencing process using stable isotopes
A DNA sequencing process using specific stable isotopes associated with specific terminators for labels. The process includes a step of incorporating a stable isotope in at least one of the...
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4997932 |
Method and kit for purifying nucleic acids
The invention teaches a method and kit for purifying nucleic acid, such as DNA, from a sample, such as lysed cell or tissue sample. A sample is applied to an anionic exchange matrix column...
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4997927 |
Improved process for the purfication of synthetic oligonucleotides
A process for the purfication of synthetic oligonucleotides is described which drastically simplifies the hitherto very time consuming purification process. The deprotection solution is adsorbed...
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4961915 |
Automatic chemistry machine
An automatic chemistry machine particularly suitable for the automatic sequencing of DNA on the microliter scale including a source of reagents, a common means to transfer by contact microliter...
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4959463 |
Intermediates
A method is provided for the high fidelity, rapid and economical in vitro synthesis of oligonucleotides. Nucleoside H-phosphonates are condensed in seriatim using a dehydrating agent to produce a...
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4950745 |
Process for synthesis of oligonucleotides and compound for forming polymeric protecting group
A chain-extended oligonucleotide is synthesized by condensing a mononucleotide, oligonucleotide, mononucleoside succinate or oligonucleotide succinate in which only the terminal 5'-hydroxyl group...
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4942227 |
Bifunctional molecules having a DNA intercalator or DNA groove binder linked to ethylene diamine tetraacetic acid, their preparation and use to cleave DNA
Bifunctional molecules having a DNA intercalator or DNA groove binder linked to ethylene diamine tetraacetic acid such as compounds having the formula: ##STR1## wherein R is methyl or ethyl. Also,...
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4935342 |
Method of isolating and purifying nucleic acids from biological samples
The method of this invention is applicable to rapid separation, isolation, and purification of DNA or RNA from biological samples. The DNA/RNA may be in double-stranded or single-stranded form. The...
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4923978 |
Process for purifying nucleic acids
The process for separating the proteinaceous materials from nucleic acids involves contacting a solution containing the proteinaceous materials and nucleic acids with a solid phase extraction...
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4921952 |
Nucleic acid isolation process
A method is provided for isolating DNA from eukaryotic cell and flow sorted chromosomes. When DNA is removed from chromosome and cell structure, detergent and proteolytic digestion products remain...
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4913902 |
Purification by affinity binding to liposomes
A method of extracting a compound specifically bound by a ligand from a crude solution containing the compound is disclosed. The method comprises providing a multiplicity of liposomes having the...
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4863847 |
Process for directly determining apurinic and apyrimidinic sites in DNA
A simple and fast process is described which serves to directly determine apurinic and apyrimidinic sites (AP sites) in DNA using [ 14 C]methoxyamine which, after loss of a purine or pyrimidine...
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4863848 |
Double-stranded vector and process using it
The invention concerns a double-stranded recombinant DNA cloning vector comprising one single insertion site for foreign DNA and one or two labelling sites adjacent or approximate to said insertion...
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4855225 |
Method of detecting electrophoretically separated oligonucleotides
A method is provided for detecting up to four classes of oligonucleotides which have been separated by gel electrophoresis. The method entails labeling members of each class of oligonucleotide with...
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4847165 |
Carrier sheets of paper and nitrocellulose bearing polyuridylic acid and polythymidylic acid residues and their use in the preparative recovery of MRNA
A carried sheet being formed of a cellulose or a nitrocellulose derivative and coated with polyuridylic acid or polythymidylic acid or a mixture thereof. A method of using the carrier sheet for...
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4830969 |
Process for the rapid and simple isolation of nucleic acids
A process for the separation from other cellular materials of heat agglomeration resistant water soluble nitrogen containing organic compounds such as plasmids, RNA's, mitochondrial DNA's, viral...
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4822731 |
Process for labeling single-stranded nucleic acids and hybridizaiton probes
Nucleic acids may be labeled by complexing the alkylating moiety of a labeling reagent into a single-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding...
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4818681 |
Fast and specific immobilization of nucleic acids to solid supports
A process for synthesizing an oligonucleotide comprising linking a nucleoside phosphate to a solid support, through the heterocyclic moiety of the nucleoside, coupling a mono- or oligonucleotide to...
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4801550 |
Method for separating and analyzing nucleic acids
The primary and/or secondary structure of nucleic acid as well as its content and molecular weight can be analyzed in solutions by light scatter or fluorescence measurements after treatment with 3...
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4795700 |
Nucleic acid probes and methods of using same
A probe and method for specific recognition or cleavage of single-stranded DNA or RNA at desired loci utilizing sequence-specific polynucleotide-chelator probes. The probe may also be utilized as a...
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4739044 |
Method for derivitization of polynucleotides
A method for the preparation of a 3' end functionalized polynucleotide is disclosed. An amine-functionalized solid phase support is treated sequentially with an anhydride, then with an...
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4699717 |
Chromatographic process for the separation of nucleic acids
A process for the chromatographic separation of nucleic acid using a chromatographic carrier material is described in which the surface of the carrier material is specially modified.
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4670379 |
Polynucleotide hydridization assays employing catalyzed luminescence
Polynucleotide hydridization assays employing catalyzed luminescence.
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4666829 |
Polypeptide marker for Alzheimer's disease and its use for diagnosis
A novel polypeptide, Alzheimer's Amyloid Polypeptide (AAP), is provided in substantially purified form which is isolated from amyloid deposits in patients with Alzheimer's Disease. The polypeptide...
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4647529 |
Hybridization method of detecting nucleic acid sequences with probe containing thionucleotide
Improved hybridization probe compositions and method for detecting nucleic acids are provided. A hybridization probe constructed to contain thionucleotides is hybridized to a nucleotide sequence of...
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4626501 |
Labeled DNA
Polymeric, single-stranded DNA molecule which is labeled with a plurality of labels, the labels being bonded, via linking groups, to adenine and cytosine bases of the DNA molecule in substantially...
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