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7385050 |
Cleavable linker for polynucleotide synthesis
Functionalized supports for polynucleotide synthesis are disclosed. The supports have linker moieties that are stable to conditions used in polynucleotide synthesis, but may be cleaved to release...
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7381820 |
α-1-phosphorylated-2-deoxy-2-fluoroarabinoside and process for producing 2′-deoxy-2′-fluoro-β-d-arabinonucleoside
A method for producing 2′-deoxy-2′-fluoro-β-D-arabinonucleoside represented by formula (II):
(wherein B represents a base), in particular,...
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7378516 |
Synthesis of sulfurized oligonucleotides
Methods for the formation of sulfurized oligonucleotides are provided. The methods allow for the formation of phosphorothioate linkages in the oligonucleotides or derivatives, without the need for...
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7355037 |
Thermolabile hydroxyl protecting groups and methods of use
Provided is a hydroxyl-protected alcohol of the formula R—O—Pg, wherein Pg is a protecting group of the formula:
wherein Y, Z, W, R 1 , R 1a , R 2 , R 2a , R 3 , R 3a , R 4 , R 4a , a,...
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7348391 |
Polymeric beads for oligomer synthesis
The present invention provides solid support media for use in oligomer synthesis, methods of producing the media, and methods of using the media. In some embodiments, the processes of the invention...
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7339052 |
Phosphoramidite activator for oligonucleotide synthesis
The present invention provides an improved phosphoramidite activator comprising a solution of 5-benzylmercaptotetrazole in acetonitrile and N-alkyimidazole. Further provided are improved methods of...
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7276599 |
Oligonucleotide synthesis with alternative solvents
The invention provides for methods of manufacturing an oligonucleotide comprising a pentavalent phosphate triester. In particular, the method comprises providing a 5′ blocked-nucleoside,...
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7271258 |
Methods of synthesizing oligonucleotides using carbonate protecting groups and alpha-effect nucleophile deprotection
The invention provides methods for synthesizing oligonucleotides using nucleoside monomers having carbonate protected hydroxyl groups that are deprotected with α-effect nucleophiles. The α-effect...
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7227017 |
Process for the preparation of phosphorothioate oligonucleotides
A process for the synthesis of phosphorothioate oligonucleotides is provided which comprises assembling an oligonucleotide bound to a solid support in the presence of acetonitrile; prior to...
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7227016 |
Process for the synthesis of oligomeric compounds
Synthetic processes are provided wherein oligomeric compounds are prepared having phosphodiester, phosphorothioate, phosphorodithioate, or other covalent linkages. The oligomers have substantially...
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7217814 |
Methods of producing phosphitylated compounds
Provided are methods of producing phosphitylated compounds, including 3′-O-phosphoramidites, comprising the step of reacting a hydroxyl-containing compound with a phosphitylating agent in the...
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7193077 |
Exocyclic amine triaryl methyl protecting groups in two-step polynucleotide synthesis
Precursors for use in the synthesis of polynucleotides and methods of using the precursors in synthesizing polynucleotides are disclosed. The precursors include a heterocyclic base having an...
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7169916 |
Chloral-free DCA in oligonucleotide synthesis
A process of manufacturing oligonucleotides includes a 5′-deblocking step in which the 5-blocking group is removed with dichloroacetic acid that is essentially free of chloral. The process is...
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7153954 |
Method for preparation of LNA phosphoramidites
The present invention relates to large scale preparation of LNA phosphoramidites using a 2-cyanoethyl-N,N,N′,N′-tetra-substituted phosphoramidite and a nucleophilic activator, e.g....
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7144700 |
Photolithographic solid-phase polymer synthesis
Methods, employing a polycyclic hydrocarbon or a polycyclic heteroaromatic compound as sensitizers, are provided to increase the efficiency of removing, by irradiation, photolabile protecting...
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7135565 |
Synthesis of polynucleotides using combined oxidation/deprotection chemistry
A method of synthesizing a polynucleotide which can, for example, be used during fabrication of an array. A second nucleoside is coupled to a first nucleoside through a phosphite linkage, with the...
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7135564 |
Reusable solid support for oligonucleotide synthesis
A reusable linker arm for solid support oligonucleotide synthesis, the linker arm comprising formula (a) wherein Z is a linker moiety and T is an organic radical. A method for adding one or more...
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7125982 |
Microbial production of nuclease resistant DNA, RNA, and oligo mixtures
The present method describes the use of thio-phosphate as a feed source for micro-organisms and multi-cellular organisms. This compound enters into nucleotide pools and ultimately into polymers of...
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RE39324 |
Pyrimidine derivatives and oligonucleotides containing same
Compounds having structure (1)
wherein R 1 is —H a protecting group, a linker or a binding partner; and R 2 and R 34 are as defined in the specification. The invention also provides...
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7098326 |
Methods for the integrated synthesis and purification of oligonucleotides
The present invention discloses novel methods for the integrated synthesis and purification of oligonucleotides. The methods employ novel capping reagents carrying two functional groups. The first...
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7098325 |
Process for the sulfurization of a phosphorus-containing compound
The invention relates to a process for the sulfurization of phosphorus-containing compounds which comprises contacting the phosphorus compound to be sulfurized with a sulfur transfer reagent of...
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7049432 |
Oligonucleotides having alkylphosphonate linkages and methods for their preparation
The present invention provides alkylphosphonate dimers and oligonucleotides prepared therefrom. The invention further provides novel methods for the preparation of these alkylphosphonate dimers....
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7022833 |
Process for the preparation of phosphorothioate triesters
A process for the synthesis of a phosphorothioate triester is provided. The process comprises the coupling of an H-phosphonate with an alcohol in the presence of a solution comprising both a...
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6974865 |
C3′ -methylene hydrogen phosphonate oligomers and related compounds
The present invention is directed to nucleoside monomers wherein the 3′-O atom is replaced with a methylene group. The present invention also provides oligomers comprising a plurality of such...
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6936709 |
Method for purifying protected 2′-deoxycytidines and hydrated crystals thereof
A protected 2′-deoxycytidine is purified by precipitating the protected 2′-deoxycytidine represented by general formula (3) in the form of a hydrated crystal from a solution containing the...
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6936472 |
Method for synthesizing a specific, surface-bound polymer uniformly over an element of a molecular array
A method for specifically and uniformly synthesizing desired polymers within molecular array elements. Droplets containing a reactive monomer are successively applied to the elements of a molecular...
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6919437 |
Synthetic methods and intermediates for triester oligonucleotides
Methods for the preparation of oligonucleotides having bioreversible phosphate blocking groups are disclosed. In one aspect, the present invention provides compounds comprising a sequence of...
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6911540 |
2′ Modified oligonucleotides
Oligomers which have substituents on the 2′ position are resistant to oligonucleases and furthermore can be derivatized to deliver reagents or drugs, to carry label, or to provide other properties.
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6902900 |
Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
The invention comprises novel methods and strategies to detect and/or quantify nucleic acid analytes. The methods involve nucleic acid probes with covalently conjugated dyes, which are attached...
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6900301 |
Backbone modified oligonucleotide analogues
Therapeutic oligonucleotide analogues which have improved nuclease resistance and improved cellular uptake are provided. Replacement of the normal phosphorodiester inter-sugar linkages found in...
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6875859 |
Heterocyclic compounds and their use in the detection of nucleic acids
The invention concerns compounds of the general formula (I) in which the residues R 1 to R 7 have the meanings given in the application as well as methods for their preparation. The compounds are...
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6875750 |
Aziridine compounds and methods of making and using them
Aziridine derivatives of formula (I)
are disclosed which can be used as cofactor for S-adenosyl-L-methionine-dependent methyltransferases.
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6861518 |
Preparation of phosphorothioate oligomers
Methods and Intermediates for the Preparation of Oligomers Containing Diastereomerically Enriched Phosphorothioate Linkages are Disclosed.
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6858722 |
Oligomer phosphoramidite compositions and processes for synthesizing the same
Synthetic processes are provided wherein oligomers are prepared using phosphoramidite compositions. Oligomers having phosphodiester, phosphorothioate, phosphorodithioate covalent linkages are...
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6849402 |
Method and device to quantify DNA and RNA
The invention relates to a device and a method for the detection, especially on line detection, of an amplification of a DNA and/or RNA sequence in a sample. The amplification of the DNA and/or RNA...
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6846922 |
Activators for oligonucleotide synthesis
The present invention relates to improved methods for the preparation of nucleoside phosphoramidites and oligonucleotides. In one aspect, the methods of the invention are used to prepare...
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6825339 |
Apparatus for preparing polynucleotides on a solid support
The present invention relates to an apparatus for preparing polynucleotides on a solid support in a reactor comprising a column containing an immobilized solid support that is functionalized for...
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6825338 |
Labeled oligonucleotides, methods for making same, and compounds useful therefor
Selectively functionalized oligonucleotides, methods for making same, and compounds useful therefor are disclosed. The oligonucleotides can be selectively functionalized with a first conjugate...
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6825331 |
Aminooxy functionalized oligomers, oligomer arrays and methods of using them
The present invention provides oligomers which are specifically hybridizable with a selected sequence of RNA or DNA wherein at least one of the nucleoside moieties of the oligomer is modified to...
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6822088 |
Synthesis of oligonucleotides on solid support
The present invention relates to methods for synthesizing an oligomeric compound on a solid support comprising contacting a solid support with a filling material to produce a mixture thereof;...
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6790945 |
Fluorescent quenching detection reagents and methods
Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
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6768005 |
Process
A process for the synthesis of phosphorothioate oligonucleotides is provided which comprises assembling an oligonucleotide bound to a solid support in the presence of acetonitrile; prior to...
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6762298 |
Thermolabile phosphorus protecting groups, associated intermediates and methods of use
The invention provides a method of thermally deprotecting the internucleosidic phosphorus linkage of an oligonucleotide, which method comprises heating a protected oligonucleotide in a fluid medium...
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6696555 |
3'-Desoxypentopyranosyl nucleic acid, its production and its use
The invention relates to a 3′-deoxypentopyranosylnucleic acid consisting essentially of 3′-deoxypentopyranosylnucleosides of the formulae (I) or of the formulae (II) their preparation and...
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6664388 |
Reagents for oligonucleotide cleavage and deprotection
The present invention provides a process for the removal of protecting groups, i.e. deprotection, from chemically synthesized oligonucleotides. In one embodiment, the invention provides reagents...
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6653458 |
Modified oligonucleotides
Modified oligonucleotides containing at least one 2′,5′-internucleotide linkage are provided. The oligonucleotides of the invention may also bear additional substituents at the 2′- and...
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6649750 |
Process for the preparation of oligonucleotide compounds
Synthetic processes are provided wherein high purity oligomers are prepared using support bound phosphoramidite protocols starting with a nucleoside or larger synthon linked to a support media...
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6646118 |
Solid phase synthesis
The present invention relates to a support system for solid phase synthesis of oligomers, such as oligonucleotides, wherein the starting compound is bound to the support via a disiloxyl linkage....
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6630581 |
Monomers for solid phase synthesis of oligonucleotides using carbonate protecting groups and alpha-effect nucleophile deprotection
The invention provides a method for synthesizing oligonucleotides using carbonate protection of hydroxyl groups and nucleophilic deprotection reagents. The deprotection reagents irreversibly cleave...
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6617445 |
Asymmetric benzoxanthene dye labelling reagents
A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having the structure wherein Y 1 and Y 2 are individually hydroxyl, amino, imminium, or oxygen, R 1...
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