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6657052 |
Biomolecular labeling
A method for using an organic compound to label polynucleotides is described. The method utilizes an organic compound including an oligonucleotide, and electrophilic active site, an active complex,...
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6653462 |
Nucleotide compounds including a rigid linker
A nucleoside/tide compound having the structure: NUC-L-S-LB/LG is described wherein NUC is a nucleoside/tide having a nucleobase portion B, L is a rigid linkage, S is a spacer; and LB/LG is a...
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6653458 |
Modified oligonucleotides
Modified oligonucleotides containing at least one 2′,5′-internucleotide linkage are provided. The oligonucleotides of the invention may also bear additional substituents at the 2′- and...
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6649598 |
4,7-dichlorofluorescein dyes as molecular probes
Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting specially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly...
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6646243 |
Nucleic acid reading and analysis system
A method and apparatus for preparation of a substrate containing a plurality of sequences. Photoremovable groups are attached to a surface of a substrate. Selected regions of the substrate are...
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6642001 |
Generic SBE-FRET protocol
The present invention is drawn to methods for detection, quantitation and analysis of nucleotides of interest in nucleic acid sequences of interest using single base extension and fluorescence...
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6635418 |
Assay methods for nucleic acid in a sample
The present invention relates to a method for the determination of the presence and amount of DNA in a sample. The method is based on the use of a nucleic acid template dependent enzyme in...
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6635452 |
Releasable nonvolatile mass label molecules
Using nonvolatile, releasable, mass-labels, the present invention provides for the synthesis and use of mass-labeled compounds to specifically interact with biomolecular targets. Following binding...
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6632606 |
Methods for single nucleotide polymorphism detection
Methods and compositions are provided for determining large numbers of single nucleotide polymorphisms in target DNA employing particles having (1) primers complementary to sequences in the target...
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6627748 |
Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
This invention provides a combinatorial fluorescence energy transfer tag which comprises a plurality of fluorescent molecules, comprising one or more energy transfer donor and one or more energy...
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6617106 |
Methods for preparing oligonucleotides containing non-standard nucleotides
The disclosure describes building blocks for preparing oligonucleotides carrying non-standard nucleobases that can pair with complementary non-standard nucleobases so as to fit the Watson-Crick...
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6610487 |
Method of diagnosing gummy stem blight in plants using a polymerase chain reaction assay
The present invention provides a sensitive test for objectively diagnosing the presence of Didymella bryoniae , the causative agent of gummy stem blight, and differentiating it from similar,...
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6605434 |
Direct bacterial lysate sequencing
The present invention relates to novel methods for sequencing nucleic acid molecules. More specifically, methods are provided for sequencing nucleic acid molecules present in bacterial lysates...
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6593465 |
Method for single-step attachment of a label to target molecules
Compounds and methods are provided for a single-step covalent attachment of a label to a molecule comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then,...
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6593464 |
Method for deblocking of labeled oligonucleotides
The invention relates to a process for deblocking substantially a blocked, detectably labeled oligonucleotide by contacting the blocked detectably labeled oligonucleotide with an effective amount...
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6589730 |
Methods for identifying protein-protein interactions by selective transduction
A method for identifying specific protein-protein (i.e., ligand/anti-ligand) interactions that internalize and are detected by transgene expression. A ligand displaying genetic package that carries...
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6589736 |
Photocleavable agents and conjugates for the detection and isolation of biomolecules
This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily...
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6583122 |
Nucleosides and oligonucleotides containing boron clusters
Carboranyl-containing nucleosides and oligonucleotides are provided for use in boron neutron capture therapy (BNCT) and for other therapeutic and diagnostic purposes.
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6576426 |
Tag reagent and assay method
This invention provides reagents, libraries and sets of the reagents, and assay methods using the reagents, the reagents comprising an analyte moiety and a tag moiety, wherein the tag moiety...
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6573374 |
Nucleotides labelled with an infrared dye and their use in nucleic acid detection
Nucleoside-5′-triphosphates and phosphoramidites which carry a residue absorbing in the long wavelength region, preferably a carbocyanine group of the general formula (I), on the base portion or...
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6562959 |
Detection of altered expression of genes regulating cell proliferation
The present invention relates to fluorescent probes which can be used in multicolor fluorescence in situ hybridization, and mainly chromosome painting. The probes intended for labeling a chromosome...
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6562955 |
Oligonucleotides for detection of Vibrio parahaemolyticus and detection method for Vibrio parahaemolyticus using the same oligonucleotides
An oligonucleotide for detection or amplification of a gene selected from the group consisting of Vibrio parahaemolyticus thermostable direct hemolysin-related hemolysin genes (trh1 and trh2) and...
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6562567 |
Method of detecting a nucleic acid
Disclosed is a method of detecting specific nucleic acids using an oligonucleotide linked to a cleavable tag. The presence of a specific nucleic acid in a population of nucleic acids is determined...
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6555317 |
Detection of differences in nucleic acids
A method is disclosed for detecting the presence of a difference between two related nucleic acid sequences. In the method a complex is formed comprising both strands of each sequence. Each member...
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6544738 |
Detectably and removably tagged nucleic acids
Disclosed is a method of detecting specific nucleic acids using an oligonucleotide linked to a cleavable tag. The presence of a specific nucleic acid in a population of nucleic acids is determined...
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6531282 |
Multiplex amplification and analysis of selected STR loci
The present invention provides a means to identify the alleles present in a DNA-containing sample by providing subsets of loci for amplification by multiplex PCR. The loci include the thirteen...
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6531581 |
Purines and pyrimidines linked to a quencher
Compounds are described comprising purines substituted at the C-8 position and pyrimidines substituted at the C-4 position with a linker and quencher. These compounds, when incorporated into...
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6525183 |
Multiple-labelled oligonucleotides synthesized on solid-supports
Methods and compositions to label oligonucleotides and analogs directly on a solid-support having the structure where S is a solid-support, A is a cleavable linker, X is a moiety with three or...
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6524798 |
High efficiency methods for combined immunocytochemistry and in-situ hybridization
The invention provides a high efficiency method for combined immunocytochemistry and in situ hybridization. In one aspect, the method is used to simultaneously determining a cell phenotype and...
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6524829 |
Method for DNA- or RNA-sequencing
The invention relates to methods for the base sequencing of deoxyribonucleic acid or ribonucleic acid, comprising the following steps: (1) immobilising DNA or RNA single strands on a planar...
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6514768 |
Replicable probe array
A system for producing substantially identical specific binding ligand (e.g., nucleic acid) probe arrays, for instance, by preparing and replicating an original master array and/or by providing a...
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6511804 |
Selective assay for determining the identity of live microorganisms in a mixed culture
The invention provides a method for determining the identity of an unknown live microorganism in a mixed culture. The microorganism can be a bacterium, fungus, virus, or protozoan. The invention...
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6506895 |
Photoactivatable nucleic acids
A photoactivatable nucleic acid derivative composition in which one or more photoreactive group(s) are bound to a natural or synthetic nucleic acid. The photoreactive groups can be bound to the...
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6503720 |
Method for quantification of an analyte
Methods are provided for quantifying the concentration of a nucleic acid in a nucleic acid sample. The methods include contacting the nucleic acid sample with an amplifying agent, amplifying at...
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6498245 |
Nucleic acid-immobilized substrate
By immobilizing identical or different nucleic acids in a plurality of dot-like areas on a carrier which comprises a base material and compound carried on the base material, the compound having one...
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6489097 |
Method for the detection of telomerase activity
A method for the detection of telomerase activity, for the detection of cancer cells and for the diagnosis of cancer comprising amplifying an oligonucleotide sequence extended by a DNA extension...
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6486308 |
Covalently linked oligonucleotide minor groove binder conjugates
Minor groove binding molecules are covalently bound to oligonucleotides which in their base sequence are complementary to a target sequence of single stranded or double stranded DNA, RNA or hybrids...
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6479262 |
Solid phase enzymatic assembly of polynucleotides
The present invention provides methods of preparing large polynucleotides of arbitrary sequence and in a manner that will readily lend itself to automation. The present invention provides methods...
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6472156 |
Homogeneous multiplex hybridization analysis by color and Tm
The invention provides methods and devices for analyzing sequence variations in nucleic acid samples comprising multiple loci, each having two, three or more possible allelic sequences. The method...
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6472146 |
Methods for identification on internalizing ligands and identification of known and putative ligands
A method for identifying target cells and tissues which internalize known or putative ligands is provided. A ligand displaying genetic package that carries a reporter or selectable marker and...
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6468740 |
Cyclic and substituted immobilized molecular synthesis
A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed...
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6465644 |
Sulfonated &lsqb 8,9&rsqb benzophenoxazine dyes and the use of their labelled conjugates
Fluorescent, sulfonated 3,7-diamino-[8,9]benzophenoxazine dyes are provided that are especially useful for labelling biopolymers and other substrates. The dye-labelled conjugates can be used in a...
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6455248 |
Reagents and kits for detecting fungal pathogens in a biological sample
The present invention provides novel methods and materials for detecting the presence of a fungus in a biological sample. The inventive methods and materials exploit the fact that the amino acid...
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6455684 |
Insitu assay of substance in biological sample using labeled probe
A method for analyzing an objective substance, comprising reacting a labeled probe with an objective substance on a biological sample, said probe comprising a label substance of the formula (I): ...
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6448008 |
Fluorescent cyanine labels containing a sulfamido linker arm
A fluorescent cyanine dye of the following general formula is disclosed: wherein: X 1 and X 2 are independently selected from the group consisting of —O—, —S—, —C(CH 3 ) 2 or...
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6448015 |
Probe for detecting point mutations in DNA utilizing fluorescence energy transfer
A method for detecting point mutations in DNA using a fluorescently labeled oligomeric probe and Forster resonance energy transfer (FRET) is disclosed. The selected probe is initially labeled at...
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6440671 |
Use of continuous/contiguous stacking hybridization as a diagnostic tool
A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease...
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6441152 |
Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
This invention pertains to methods, kits and compositions suitable for the detection, identification and/or quantitation of nucleic acids which are electrostatically immobilized to matrices using...
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6426183 |
Oligonucleotide microarrays: direct covalent attachment to glass
The present invention provides an improved method for stably attaching a desired compound to a silaceous or silane-containing substrate, in particular a glass, porous silica, or oxidized silicon....
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6420112 |
DNA attachment to support structures
Microscopic beads or other structures are attached to nucleic acids (DNA) using a terminal transferase. The transferase adds labeled dideoxy nucleotide bases to the ends of linear strands of DNA....
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