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6875858 |
Functionalized polynucleotide compound, optionally marked and method for detecting a target nucleic acid
The invention relates to a functionalized compound of general formula (I):
in which W represents a nucleotide analog, L represents a linker arm comprising at least four atoms, R 1 ...
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6875750 |
Aziridine compounds and methods of making and using them
Aziridine derivatives of formula (I)
are disclosed which can be used as cofactor for S-adenosyl-L-methionine-dependent methyltransferases.
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6872532 |
Method and kit for detecting white spot syndrome virus
A method for detecting white spot syndrome virus (WSSV) in a sample includes the steps of (a) adding to the sample a thermostable polymerase, appropriate nucleoside triphosphates, a...
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6861514 |
Nucleosidic and non-nucleosidic folate conjugates
Oligonucleotide-folate conjugates are described wherein folates are conjugated to one or more sites on an oligonucleotide including the 2′-, 3′-, 5′-, nucleobase and internucleotide linkage...
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6861220 |
Genetic screening methods
A method for screening molecule which have a synthetic lethal property when in combination with a gene of interest carrying a non-lethal mutation, said method comprising the steps of: transfecting...
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6844155 |
Methods for detecting contamination in molecular diagnostics using PCR
The invention provides methods for detecting contamination in a PCR reaction. Methods of the invention are especially useful for detection of contamination in heterogeneous samples containing a...
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6841128 |
DNA base sequencing system
The present invention provides a DNA base sequencing system having a compact, simple and convenient structure. In one embodiment of the present invention, a reaction chamber module for...
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6835827 |
Labelled oligonucleotides synthesized on solid-supports
Methods and compositions to label oligonucleotides and analogs directly on a solid-support having the structure where S is a solid-support, A is a cleavable linker, X is a moiety with three or...
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6835824 |
Peanut allergens and methods
One of the major peanut allergens, Ara h I, was selected from cDNA expression library clones using Ara h I specific oligo-nucleotides and polymerase chain reaction technology. The Ara h I clone...
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6833450 |
Phosphite ester oxidation in nucleic acid array preparation
Methods are provided for preparing nucleic acid arrays on a support. In these methods a plurality of nucleic acids are synthesized on the support and the synthesis steps include oxidizing a...
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6828142 |
Polynucleotide strands operably linked to nanocrystals functionalized to be water soluble
Provided are compositions and assay kits comprising functionalized nanocrystals having extending therefrom a plurality of polynucleotide strands of known sequence; wherein primary dots are used to...
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6825338 |
Labeled oligonucleotides, methods for making same, and compounds useful therefor
Selectively functionalized oligonucleotides, methods for making same, and compounds useful therefor are disclosed. The oligonucleotides can be selectively functionalized with a first conjugate...
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6821727 |
Hybridization assay using self-quenching fluorescence probe
A hybridization assay is provided which uses an oligonucleotide probe which includes a fluorescent reporter molecule and a quencher molecule capable of quenching the fluorescence of the reporter...
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6818760 |
Removal of dye-labeled dideoxy terminators from DNA sequencing reactions
This invention provides methods for removing unincorporated dye-labeled molecules from a mixture that includes dye-labeled polynucleotides or other polymers and the unincorporated dye-labeled...
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6818758 |
Estrogen receptor beta variants and methods of detection thereof
The present invention is based on sequencing genomic DNA from human chromosome 6 and cDNAs to define the genomic structure of estrogen receptor beta genes and novel polymorphism in the estrogen...
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6818399 |
Methods employing generalized target-binding e-tag probes
Methods for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a...
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6815167 |
Amplification of DNA to produce single-stranded product of defined sequence and length
The present disclosure relates to methods for generating single-stranded DNA molecules of defined sequence and length. Specifically, a region of template containing target sequence is amplified by...
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6809195 |
Process for the preparation of oligonucleotides
The present invention discloses methods for synthesizing oligomeric compounds. The methods include a modified phosphoramidite protocol wherein the oxidation and capping steps are combined into a...
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6803201 |
Compositions and methods for polynucleotide sequence determination
The present invention relates to a method for identifying a nucleotide at a predetermined location on a target polynucleotide. The method involves single nucleotide extension reaction comprising an...
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6803215 |
Sequence characterized amplified region (SCAR) test for the authentication of traditional Chinese medicinal materials
Methods are provided for the authentication of traditional ingredients in Chinese medical materials. Arbitrarily primed polymerase chain reaction techniques are employed to identify genomic regions...
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6803198 |
Carbamate-derivatized nucleosides and oligonucleosides
Nucleosides and oligonucleosides functionalized to include carbamate functionality, and derivatives thereof. In certain embodiments, the compounds of the invention further include steroids,...
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6800751 |
Reagent and process for protecting nucleoside hydroxyl groups
Silylating reagents having a group other than a divalent oxygen separating two silyl groups provide regioselective protection of reactive groups under robust conditions, such as basic conditions...
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6794142 |
Amplification using modified primers
The present invention provides modified primers for use in the amplification of a nucleic acid sequence. Amplifications carried out using the modified primers result in less template-independent...
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6790945 |
Fluorescent quenching detection reagents and methods
Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
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6787311 |
Diagnosis of premature ovarian failure
Methods by which a predisposition to Premature Ovarian failure (POF) can be determined. In particular, methods are provided for detecting whether a female has a predisposition to POF with reference...
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6783934 |
Methods for quantitative analysis of nucleic acid amplification reaction
A method for determining a threshold cycle number or time value in a nucleic acid amplification reaction comprising the steps of amplifying a nucleic acid sequence in a reaction mixture; at a...
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6783944 |
Genetic changes in atypical nodular proliferations in congenital melanocytic nevi
The invention provides methods of distinguishing benign growths arising from congenital melanocytic nevi from malignant melanoma. The methods comprise detecting a change in chromosome number that...
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6777189 |
Nucleic acid analysis using non-templated nucleotide addition
One embodiment of the invention is a method of producing an oligonucleotide extended by a single nucleotide base. An oligonucleotide and an extension terminating nucleotide are mixed with an enzyme...
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6770439 |
Sets of generalized target-binding e-tag probes
Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a...
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6764821 |
Detecting the presence of specific target nucleic acid sequences through stem-loop formation
This invention provides novel processes for amplifying nucleic acid sequences of interest, including linear and non-linear amplification. In linear amplification, a single initial primer or nucleic...
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6762292 |
Optimally labeled oligonucleotides
A method for the preparation of optimally labeled oligonucleotides wherein label-conjugated nucleotide triphosphates are incorporated into a nucleic acid sequence in a defined repetitive manner...
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6762023 |
Methods for identifying individuals at risk of developing osteoporosis
Methods and kits for identifying individuals at risk of developing osteoporosis are provided. These methods and kits are based on detecting the presence of polymorphisms in the tumor necrosis...
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6747143 |
Methods for polymer synthesis
A method and apparatus for preparation of a substrate containing a plurality of sequences. Photoremovable groups are attached to a surface of a substrate. Selected regions of the substrate are...
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6743605 |
Linear amplification of specific nucleic acid sequences
This invention provides novel processes for amplifying nucleic acid sequences of interest, including linear and non-linear amplification. In linear amplification, a single initial primer or nucleic...
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6743902 |
Sugar modified nucleosides
A number of modified nucleosides are disclosed composed of modified sugar moieties which contain substituents at C1 and C4 positions, or branched substituents at C3 and C5 positions of deoxyribose...
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6734297 |
Oligonucleotide labeling reagents
A labeling reagent for use in oligonucleotide (“oligo”) synthesis, as well as a method of preparing such a labeling reagent, a method of using such a reagent for synthesizing a labeled...
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6733976 |
Detection of bacterial kidney disease
A real-time PCR method for detecting and quantifying the presence or absence of Renibacterium salmoninarum , the causative organism of Bacterial Kidney Disease, in a sample. The method includes...
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6727356 |
Fluorescent quenching detection reagents and methods
Oligonucleotide-fluorophore-quencher conjugates wherein the fluorophore moiety has emission wavelengths in the range of about 300 to about 800 nm, and or where the quencher includes a substituted...
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6720148 |
Methods and systems for identifying nucleotides by primer extension
The methods and systems for identifying nucleotides monitor changes in fluorescent signal that occur during the polymerase-mediated extension of dye labeled primer molecules. In certain aspects,...
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6713622 |
4,7-dichlororhodamine dye labeled polynucleotides
A set of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structures wherein R 1 -R 6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower...
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6706481 |
In vitro selection of signaling aptamers
The present invention provides a method for the in vitro selection of signaling aptamers comprising the steps of synthesizing a DNA pool, the DNA having a random insert of nucleotides of a specific...
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6706476 |
Process for amplifying and labeling single stranded cDNA by 5′ ligated adaptor mediated amplification
This invention relates to a method wherein special adaptors and primers are used for single stranded cDNA amplification. The method comprises contacting RNA with an anchored cDNA synthesis primer...
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6699693 |
Process for DNA replication
A method is provided for replicating DNA, and in particular for replicating large segments of DNA. A primer is combined with a target DNA molecule to be replicated. The primer is designed to be at...
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6699670 |
Quantitative assay for the simultaneous detection and speciation of bacterial infections
An adaptation of the real-time PCR assay allows for highly sensitive detection of any eubacterial species with simultaneous speciation. The assay relies on a ‘multiprobe’ design in which a...
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6686152 |
Methods employing oligonucleotide-binding e-tag probes
Methods for the multiplexed detection of known, selected nucleotide target sequences are provided. Detection involves the release of identifying tags as a consequence of target recognition. The...
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6682887 |
Detection using degradation of a tagged sequence
Methods and compositions are provided for detecting DNA sequences, particularly single nucleotide polymorphisms, using a pair of nucleotide sequences, a primer and a snp detection sequence, where...
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6673550 |
Electrophoretic tag reagents comprising fluorescent compounds
Electrophoretic probes comprising fluorescent compounds as detection groups and mobility modifiers are disclosed for the multiplexed detection of the binding of, or interaction between, one or more...
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6670126 |
Poly-primed amplification of nucleic acid sequences
Methods for the amplification of selected nucleic acids at high rate but with high specificity and control are disclosed using a secondary, tertiary, quaternary or higher order platform especially...
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6664388 |
Reagents for oligonucleotide cleavage and deprotection
The present invention provides a process for the removal of protecting groups, i.e. deprotection, from chemically synthesized oligonucleotides. In one embodiment, the invention provides reagents...
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6660479 |
Process for preparing peptide nucleic acid probe using polymeric photoacid generator
Disclosed is a process for preparing arrays of oligopeptide nucleic acid probes immobilized on a solid matrix by employing polymeric photoacid generator. Arrays of peptide nucleic acid probes of...
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