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5506350 |
Production of chromosome region specific DNA sequences and transamination
Techniques for producing cloned DNA sequences are provided which sequences are complementary to DNA occurring in one selected region of one chromosome of a multi-chromosomal genome, such as the...
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5491063 |
Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
The present invention is directed to methods for controlling the light emission of an oligonucleotide labeled with a light-emitting label that are useful in nucleic acid detection assays. A...
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5482836 |
DNA purification by triplex-affinity capture and affinity capture electrophoresis
The invention provides a method for purifying or isolating double stranded DNA intact using triple helix formation. The method includes the steps of complexing an oligonucleotide and double...
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5474895 |
Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor
Methods for the detection of nucleic acid using sandwich hybridization are provided. The target nucleic acids are captured by hybridization with oligonucleotides covalently attached to polystyrene...
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5470707 |
Hydrogen bond labeling and base sequence determination methods for DNA or RNA
A hydrogen bond labeling and base sequence determination method for DNA or RNA takes advantage of the complementary nature of the fundamental structure of DNA or RNA bases to bind corresponding...
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5453505 |
N-heteroaromatic ion and iminium ion substituted cyanine dyes for use as fluorescence labels
The present invention relates to iminium ion substituted cyanine dyes having a fluoresence absorbance of between about 500 and 900 nm, a reduced tendency to aggregate and enhanced photostability....
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5449767 |
Modified polynucleotides and methods of preparing same
Compounds having the structure: ##STR1## wherein B represents 7-deazapurine, or pyrimidine moiety covalently bonded to the C 1 '-position of the sugar moiety, provided that when B is...
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5446137 |
Oligonucleotides containing 4'-substituted nucleotides
This invention relates to oligonucleotides having at least one nucleotide that is substituted at the 4' position of the sugar moiety with a substituent other than hydrogen. These oligonucleotides...
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5442045 |
Biological conjugates of fluorescent rhodol dyes
Novel fluorescent dyes based on the rhodol structure are provided. The new reagents contain functional groups capable of forming a stable fluorescent product with functional groups typically found...
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5414077 |
Non-nucleoside linkers for convenient attachment of labels to oligonucleotides using standard synthetic methods
Pseudonucleosides and pseudonucleotides are useful in the synthesis of oligomers which contain these components as a means to derivatize the resulting oligonucleotide to useful substituents such as...
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5410030 |
Dimers of unsymmetrical cyanine dyes containing pyridinium moieties
The invention relates to nucleic acid stains that are homo- or heterodimers of unsymmetrical cyanine dyes, where at least one dye unit of the dimer is a substituted or unsubstituted benzazolium- or...
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5407795 |
CMV probes for use in solution phase sandwich
Novel DNA probe sequences for detection of CMV in a sample in a solution phase sandwich hybridization assay are described. Amplified nucleic acid hybridization assays using the probes are exemplified.
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5407801 |
Formation of oligonucleotide triplexes with selectively modified cytosines
An oligonucleotide or analog thereof including a single cytidine residue at a selected position and having one or more cytidine residues which are 5-methyl substituted. The cytidine nucleus can be...
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5405950 |
N.sup.4 -substituted cytidines and process for incorporation into an oligonucleotides
An oligonucleotide containing at least one cytidine analog labeled at C4 with a reporter group.
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5403708 |
Methods and compositions for determining the sequence of nucleic acids
Methods and compositions for forming ligation product hybridized to a nucleic acid template. The ligation product is formed by contacting a nucleic acid template with a primer capable of...
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5401837 |
Method for labeling the 3' terminus of a synthetic oligonucleotide using a unique multifunctional controlled pore glass (MF-CPG) reagent in solid phase oligonucleotide synthesis
A method for derivatizing and labeling the 3'-terminus of an oligonucleotide during solid phase synthesis comprising the use of a multifunctional reagent whose preferred structure is shown below....
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5391785 |
Intermediates for providing functional groups on the 5' end of oligonucleotides
Phosphoramidites of the formula ##STR1## where R is a base-labile protecting group, R 1 and R 2 are individually alkyl of 1 to 6 carbon atoms, cycloalkyl of 3 to 8 carbon atoms, or aryl of 6 to...
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5384241 |
Specific binding assay compound with inhibitive self-quenching characteristics
Disclosed is an assay system including a compound comprising an analyte-specific moiety having substituted thereon a polymer comprising plurality of self-quenching emitter moieties and a plurality...
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5366860 |
Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
A spectrally resolvable set of rhodamine dyes are provided for use in the chain termination method of nucleic acid sequencing. A different rhodamine dye from the group consisting of...
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5328824 |
Methods of using labeled nucleotides
Compounds having the structure: ##STR1## wherein B represents a purine, 7-deazapurine, or pyrimidine moiety covalently bonded to the C 1 ' -position of the sugar moiety, provided that when B is...
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5321131 |
Site-specific functionalization of oligodeoxynucleotides for non-radioactive labelling
Disclosed are compounds consisting of a plurality of nucleosides which are covalently linked by at least one aminoalkylphosphoramidate linkage of the formula ##STR1## wherein n=2 to 6 and Nu 1 and...
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5319079 |
Process for terminal substituting of a polynucleotide
Method for substituting a substitution compound for the hydrogen of a support bound primary hydroxyl group. The support bound primary hydroxyl group is treated in an organic, substantially...
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5312921 |
Dyes designed for high sensitivity detection of double-stranded DNA
Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon...
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5290925 |
Methods, kits, and reactive supports for 3' labeling of oligonucleotides
In a first aspect, the invention involves a reactive support useful for automated synthesis of oligonucleotides. The reactive support comprises a label moiety (e.g. hapten) covalently bonded via a...
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5283174 |
Homogenous protection assay
Improved homogenous diagnostic assay methods and labels for detecting an analyte in a medium when the analyte is a member of a specific binding pair. The methods and labels provide procedures for...
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5262526 |
Fluorescent compound, complex, reagent, and specific binding assay employing said reagent
A fluorescent compound, which is readily capable of forming a stable complex with a rare-earth metal ion having fluorescence with satisfactory intensity even in an aqueous system and long...
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5262536 |
Reagents for the preparation of 5'-tagged oligonucleotides
Fluorescent reagents possessing an activated phosphate for the convenient covalent coupling to the 5'-hydroxyl of oligonucleotides. A class of 5'-fluorescence-tagged oligonucleptides is also...
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5260433 |
Saccharide specific binding system labeled nucleotides
The present invention provides nucleotides and polynucleotides which are chemically modified or labeled so as to be capable of ready detection when attached to and/or incorporated in nucleic acid...
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5258506 |
Photolabile reagents for incorporation into oligonucleotide chains
Novel photolabile photochemical reagents are disclosed. The reagents are useful in a variety of biochemical and chemical contexts, including nucleic hybridization assays and chemical...
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5258538 |
2,3-disubstituted-1,3,2-oxazaphosphacycloalkanes as nucleic acid linking agents
The compounds of the invention include novel linking agents comprising 2-substituted-3-protected-1,3,2-oxazaphosphacycloalkanes and their phosphoramidite precursors. The compounds of the invention...
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5247081 |
Protected biotin derivatives
Processes for the preparation of biotinylated polynucleotides and analogues thereof and protected intermediate products for use in such processes are described and claimed. The processes may be...
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5245022 |
Exonuclease resistant terminally substituted oligonucleotides
Compounds, compositions and methods for inhibiting gene expression are disclosed. The compounds comprise oligonucleotide sequences of from about 9 to about 200 bases having a diol at either or both...
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5241060 |
Base moiety-labeled detectable nucleatide
The present invention provides nucleotides and polynucleotides which are chemically modified or labeled so as to be capable of ready detection when attached to and/or incorporated in nucleic acid...
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5218102 |
Nucleic acid probe containing a terminal carbamyl linking non-radioactive labeling and preparating processes
The subject of the present invention is a nucleic acid probe containing a nucleic acid sequence, wherein the said sequence is linked at its 5' end, via a divalent bifunctional chemical "arm" L, to...
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5155216 |
Nucleic acids labeled with a chemiluminescent acridine ester
Polynucleotides are labeled with chemiluminescent acridine esters or luminescent lanthanides. Labelling is preferably carried out by incorporating a functional group into a nucleotide or...
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5135717 |
Tetrabenztriazaporphyrin reagents and kits containing the same
Red-shifted, water-soluble, fluorescent, monomerically-tetherable derivatives having the formula: ##STR1## wherein, M represents either H 2 or is selected from among the following metals:...
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5128476 |
Biotinylated oligonucleotides and reagents for preparing the same
Reagents of the formula I ##STR1## wherein A is --CH 2 -- m O-- (m=2 to 20) or --C n H 2n --O-- t (n=2 to 6; t=2 to 10) and B is selected from the group consisting of ##STR2## and salts...
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5118802 |
DNA-reporter conjugates linked via the 2' or 5'-primary amino group of the 5'-terminal nucleoside
The invention consists of compounds and methods for the synthesis of oligonucleotides which contain one or more free aliphatic amino groups attached to the sugar moieties of the nucleoside...
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5112963 |
Modified oligonucleotides
The present invention provides modified oligonucleotides, wherein, amongst the first four bases on the 5'-end, they have the base sequence AT or TA and, bound to one of the two O - groups of the...
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5098996 |
Process for introducing fluorine into biologically active materials
Radioactive fluorine can be easily introduced into biologically active molecules containing amino groups. A p-bromomethyl benzoyl group is coupled to the amino group of the biologically active...
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5093245 |
Labeling by simultaneous ligation and restriction
Termini of restricted double-stranded DNA fragments are modified by ligating the fragments with terminal phosphate-free double-stranded oligonucleotides having a complementary terminus in the...
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5093232 |
Nucleic acid probes
Modified nucleotides are provided which have the structure ##STR1## wherein R 1 is a reactive group derivatizable with a detectable label, R 2 is an optional linking moiety including an amide,...
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5086068 |
Immunochemical detection of hypoxia in normal and tumor tissue
A process for preparing an immunogenic conjugate of a nitroaromatic compound and a carrier comprises colavently bonding the nitroaromatic compound to the carrier in an essentially oxygen-free...
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5082830 |
End labeled nucleotide probe
An oligo- or polynucleotide having at least one biotin directly or indirectly attached to each of the 5' and 3' end nucleotides thereof and a nucleic acid hybridization assay system for use...
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5043265 |
Inorganic phosphor labelled macromolecules; a process for their preparation and their use for immunological or immunocytochemical assays
The invention relates to macromolecules, such as proteins, including immunologically specific antibodies, lipoproteins, polynucleotides, etc., provided with luminescent labels. The invention...
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5026840 |
Photochemical nucleic acid-labeling reagent having a polyalklamine spacer
A photochemical nucleic acid-labeling reagent of the formula ##STR1## wherein Q is a photoreactive residue of a nucleic acid-binding ligand; L is a detectable label residue; R is hydrogen, C 1 to...
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5015570 |
Molecular diagnosis of Alzheimer Disease
A method for diagnosing Alzheimer Disease or a predisposition to develop Alzheimer Disease in an individual, by determining the presence of an alteration in the normal base sequence of the 24 base...
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5015569 |
Acceleration of nucleic acid hybridization
This invention relates to an improvement in nucleic acid hybridization technology. Nucleic acids bind to complementary partners in a predictable manner such that the detection of complementary...
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5013644 |
Identification of africanized honey bees
Africanized honey bees are identified by using a DNA probe capable of identifying a specific repeating gene sequence in the honey bee DNA which is a diagnostic characteristic of African honey bees...
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5013831 |
Detectable molecules, method of preparation and use
A detectable molecule of the formula A 3 --(--X--R 1 --E--Det b ) m where A 3 is A 2 or a polymer, where A 3 has at least one modifiable reactive group selected from the group consisting of...
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