|
Match
|
Document |
Document Title |
|
|
6222030 |
Solid phase synthesis of oligonucleotides using carbonate protecting groups and alpha-effect nucleophile deprotection
The invention provides a method for synthesizing oligonucleotides using carbonate protection of hydroxyl groups and nucleophilic deprotection reagents. The deprotection reagents irreversibly cleave...
|
|
|
6218108 |
Nucleoside analogs with polycyclic aromatic groups attached, methods of synthesis and uses therefor
The present invention provides fluorescent nucleosides carrying polycyclic aromatic hydrocarbons such as anthracene, phenanthrene, pyrene stilbene, tetracene or pentacene. The subject nucleosides...
|
|
|
6218530 |
Compounds and methods for detecting biomolecules
Methods are described for synthesizing compounds useful for detecting nucleic acids, and in particular, the hybridization of nucleic acids. Photocleavable agents are described, including but not...
|
|
|
6218598 |
Plant promoter
The present invention provides a promoter which is characterized in that it has following DNA sequence (a) or (b) and a DNA having the nucleotide sequence shown by SEQ ID:No.1, (a) a DNA sequence...
|
|
|
6214551 |
Oligonucleoside linkages containing adjacent nitrogen atoms
Novel compounds that mimic and/or modulate the activity of wild-type nucleic acids. In general, the compounds contain a selected nucleoside sequence wherein the nucleosides are covalently bound...
|
|
|
6211354 |
Optically active DNA probe having phosphonic diester linkage
A DNA probe of the following structural formula (1) (wherein P* is an optically active phosphorus atom, each of R 1 and R 2 is a DNA oligomer having an arbitrary sequence, and R 3 is a...
|
|
|
6207424 |
Self-primed amplification system
Disclosed are methods and compositions for copying a target nucleic acid using a self-priming primer. Single and different target nucleic acids can be copied using the disclosed methods and...
|
|
|
6207421 |
DNA sequencing and DNA terminators
A universal terminator includes a heterocycle other than naturally occuring DNA heterocycles such as adenine, cytosine, guanine and thymine. In terminating strand synthesis, the hybridized...
|
|
|
6207797 |
Method for reducing the surface reactivity of copolymers produced by electrochemical polymerization
|
|
|
6207381 |
Method for nucleic acid analysis
A method of determining the binding of an oligonucleotide probe to a test nucleic acid sequence comprises the steps of: (a) providing the test nucleic acid sequence in single-stranded form, (b)...
|
|
|
6204326 |
PNA combinatorial libraries and improved methods of synthesis
New sub-monomer synthetic methods for the preparation of peptide nucleic acid oligomeric structures are disclosed that provide for the synthesis of both predefined sequence peptide nucleic acid...
|
|
|
6200757 |
Method for controlling the extension of an oligonucleotide
The present invention relates to a method for selectively extending an oligonucleotide primer along a specific target polynucleotide sequence in a mixture of polynucleotides. A combination is...
|
|
|
6197513 |
PNA and DNA conjugates and methods for preparation thereof
Disclosed herein are methods for linking a protein or other biomolecule containing a carboxylic acid moiety to a PNA or DNA probe molecule. The methods disclosed herein involve activating the...
|
|
|
6197949 |
Electronically conductive polymer/nucleotide copolymer. preparation method therefor and use thereof
A copolymer of general formula (I), wherein unit A is a monomer of an electronically conductive polymer, unit B is a nucleotide, an oligonucleotide or an analogue thereof, x, y, z are integers of 1...
|
|
|
6197554 |
Method for generating full-length cDNA library from single cells
The present invention provides a fast, simple and specific method for generating a complete full-length cDNA library from single cells. The first reverse transcription of intracellular mRNAs with...
|
|
|
6194563 |
Solid phase nucleic acid labeling by transamination
The invention relates to a method for linking a detectable label to a nucleic acid by (1) providing a nucleic acid bound to a solid support, the nucleic acid having a cytidine base; (2)...
|
|
|
6187913 |
Covalently crosslinked oligonucleotides, preparation method and synthon which is of use in the method
A double- or single-stranded oligonucleotide comprising one or more respectively inter- or intra-oligonucleotide covalent cross-linkages, wherein the or each covalent linkage consists of an amide...
|
|
|
6187914 |
Nucleoside derivatives, and their use in oligonucleotide synthesis
The invention relates to nucleoside derivatives bearing electrolabile protector groupings and their use in an oligonucleotide synthesis method comprising at least one step of electronic deprotection.
|
|
|
6184347 |
Minimization of blooming in high-density arrays by using reactive wash reagents
A wash reagent employed for the bulk washing of the surface of a high-density array to remove unreacted reactants from cells of the array while, at the same time, reacting with the unreacted...
|
|
|
6180844 |
Composition containing fluorescence-generating substrate
A fluorescent dye-forming composition is provided that contains a fluorescence-generating substrate of formula (I): ##STR1## where R 1 and R 2 are each independently a hydrogen atom, or an...
|
|
|
6180767 |
Peptide nucleic acid conjugates
Peptide nucleic acid (PNA) oligomers are conjugated to a ligand which is capable of binding to a cell surface receptor. The ligand facilitates cellular uptake of the PNA oligomer. Where the ligand...
|
|
|
6180777 |
Synthesis of branched nucleic acids
Branched polymers and multiply connected macromolecular structures are provided, wherein the polymers and structures are provided, wherein the polymers are structures comprise at least two branches...
|
|
|
6177554 |
Nucleic acid transporter systems
Nucleic acid transporter systems for delivery of nucleic acid to a cell. The nucleic acid transporter includes a binding complex. The binding complex contains a binding molecule which...
|
|
|
6177558 |
Method and composition for chemical synthesis using high boiling point organic solvents to control evaporation
A method for reducing evaporation of a liquid reagent solution during solid phase, micro-scale chemical synthesis of a molecule comprising sub-units on an open environment solid support surface....
|
|
|
6175004 |
Process for the synthesis of oligonucleotides incorporating 2-aminoadenosine
This invention presents novel processes for incorporating 2-aminoadenosine and 2-aminoadenosine analogs into oligonucleotides. A halogenated adeninosine is incorporated into an oligonucleotide...
|
|
|
6174709 |
Method for making a primer and nucleic acid exponential amplification methods using said primer
A process for exponentially amplifying a selected nucleic acid sequence present in a sample, comprising the steps of forming a mixture of the sample and a single primer designed to hybridize with...
|
|
|
6171860 |
Antisense inhibition of rank expression
Antisense compounds, compositions and methods are provided for inhibiting the expression of RANK. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to...
|
|
|
6169176 |
Deoxynucleic alkyl thiourea compounds and uses thereof
The invention provides novel deoxynucleic alkyl thiourea (dNXt) oligonucleotide compounds for use in antisense or antigene therapy.
|
|
|
6166198 |
Methods for oligonucleotide synthesis
Improved methods for solution-phase synthesis of purified short-chain oligonucleotide coupling units, such as dimers, trimers, and tetramers, suitable for use in solution-phase or solid-phase...
|
|
|
6166197 |
Oligomeric compounds having pyrimidine nucleotide (S) with 2'and 5 substitutions
Oligonucleotide analogs are disclosed having pyrimidine monomeric sub-units therein that are modified at the 2' and 5 positions. Monomeric sub-units having these modifications may be further...
|
|
|
6165790 |
Antisense modulation of PI3 kinase p55 gamma expression
Antisense compounds, compositions and methods are provided for inhibiting the expression of PI3 kinase p55 gamma. The compositions comprise antisense compounds, particularly antisense...
|
|
|
6165786 |
Antisense modulation of nucleolin expression
Antisense compounds, compositions and methods are provided for inhibiting the expression of Nucleolin. The compositions comprise antisense compounds, particularly antisense oligonucleotides,...
|
|
|
6160109 |
Preparation of phosphorothioate and boranophosphate oligomers
Methods and intermediates for the preparation of oligomers containing diastereomerically enriched phosphorothioate and boranophosphate linkages are disclosed.
|
|
|
6156494 |
Serially directed combinatorial synthesis on fiber optic arrays
A method has been developed which utilizes functionalized optical fibers as a solid support for the assembly of combinatorial libraries of compounds. The optical fibers are used to direct light,...
|
|
|
6153742 |
General process for the preparation of cyclic oligonucleotides
The preparation process starts with a protected nucleotide which is anchored through its phosphate group with an anchoring group that links the phosphate group to a polymeric support, its...
|
|
|
6153743 |
Lithographic mask design and synthesis of diverse probes on a substrate
Systems and methods of synthesizing probes on a substrate are provided. One or more shift reticles are utilized to uniformly add monomers to the substrate at specified locations. The shift reticles...
|
|
|
6153375 |
Method of making a library of compounds using a functionalized polymer support resin affixed to a laminar material
The instant invention is directed to a method of making a library of compounds using a segmentable support material comprised of a particulate resin affixed to a porous laminar material.
|
|
|
6150147 |
Biological array fabrication methods with reduction of static charge
The present invention provides modified methods and apparatus for the preparation of arrays of material wherein each array includes a preselected collection of polymers, small molecules or...
|
|
|
6150107 |
Methods of sequencing and detection using energy transfer labels with cyanine dyes as donor chromophores
Cyanine dyes are used as the donor fluorophore in energy transfer labels in which light energy is absorbed by a donor fluorophore and transferred to an acceptor fluorophore which responds to the...
|
|
|
6150162 |
Antisense modulation of CD44 expression
Antisense compounds, compositions and methods are provided for modulating the expression of CD44. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to...
|
|
|
6143527 |
Chain reaction cloning using a bridging oligonucleotide and DNA ligase
Chain reaction cloning methods and reagents and kits for performing such methods are provided. Chain reaction cloning allows ligation of double-stranded DNA molecules by DNA ligases and bridging...
|
|
|
6143879 |
Nucleotide cleaving agents and method
The present invention provides a unique series of nucleotide cleaving agents and a method for cleaving a nucleotide sequence, whether single-stranded or double-stranded DNA or RNA, using and a...
|
|
|
6142681 |
Method and apparatus for interpreting hybridized bioelectronic DNA microarray patterns using self-scaling convergent reverberant dynamics
A technique is described for identifying mutations, if any, present in a biological sample, from a pre-selected set of known mutations. The method can be applied to DNA, RNA and peptide nucleic...
|
|
|
6143877 |
Oligonucleotides including pyrazolo[3,4-D]pyrimidine bases, bound in double stranded nucleic acids
A triplex forming oligonucleotide is complementary pursuant to the G/T or A/G recognition motif to a homopurine, or substantially homopurine target sequence in double stranded nucleic acids, and at...
|
|
|
6143531 |
Method of double stranded DNA synthesis
The present invention provides an improved method for the synthesis of double stranded DNA, particularly complementary DNA for the construction of directional complementary DNA libraries. The...
|
|
|
6140053 |
DNA sequencing by mass spectrometry via exonuclease degradation
The invention provides fast and highly accurate mass spectrometer based processes for directly sequencing a target nucleic acid (or fragments generated from the target nucleic acid), which by means...
|
|
|
6136568 |
De novo polynucleotide synthesis using rolling templates
Disclosed is a method for synthesizing polynucleotide molecules such as genes or gene segments. A primer having 5' and 3' ends is incubated with a relatively shorter template having a 5' region...
|
|
|
6136965 |
Deoxynucleic alkyl and alkoxy thiourea compounds
The invention provides novel deoxynucleic alkyl thiourea (dNXt) oligonucleotide compounds for use in antisense or antigene therapy.
|
|
|
6136543 |
Method for determining nucleic acids base sequence and apparatus therefor
A single molecule of single-stranded sample DNA (7) having a bead (5) at one end and a magnetic bead (6) at the other end is extended and fixed in the field of view of a fluorescent microscope by...
|
|
|
6132953 |
Combinatorial synthesis on soluble polyvalent supports having a discrete architecture
The present invention is directed to the use of a soluble polyvalent support for the preparation of combinatorial libraries of compounds. The resultant combinatorial libraries are useful in...
|