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9040677 Transcription activator-like effector assembly  
Described herein are techniques for assembling a polynucleotide encoding a transcription activator-like effector nucleases (TALEN). The techniques ligate and digest necessary modules for a TALEN...
9029103 Methods for sequencing polynucleotides  
Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first...
9017971 Means and methods for investigating nucleic acid sequences  
The invention provides improved methods for investigating nucleic acid sequences, wherein at least one additional probe is used which is specific for a (pseudo)gene variant of a target nucleic acid.
9012225 Lipophilic polynucleotide conjugates  
Disclosed are lipophilic polynucleotide conjugates including polynucleotide-cholesterol conjugates and methods of delivering therapeutic polynucleotides to a mammalian cell or patient in need of...
9005892 Labelling strategies for the sensitive detection of analytes  
The present invention relates to methods and reagents for detecting analytes, e.g. nucleic acids. The new methods and reagents allow a simple and sensitive detection even in complex biological...
9005985 Optical reporter compositions  
This invention provides compositions that have a light emitting reporter linked to biomolecules, preferably, nucleotide oligomers. The light reporter particles are silylated and functionalized to...
8993271 Method of nucleic acid amplification  
A nucleic acid molecule can be annealed to an appropriate immobilized primer. The primer can then be extended and the molecule and the primer can be separated from one another. The extended primer...
8987437 Processes and intermediates for preparing anti-HIV Agents  
The invention provides synthetic processes and synthetic intermediates that can be used to prepare compounds having useful anti-HIV properties.
8980584 System and methods for nucleic acid sequencing of single molecules by polymerase synthesis  
This invention relates to improved methods for sequencing and genotyping nucleic acid in a single molecule configuration. The method involves single molecule detection of fluorescent labeled PPi...
8981070 Conjugate between a thiophilic solid phase and an oligonucleotide comprising a thiooxonucleotide  
An oligonucleotide-solid phase conjugate, wherein the solid phase is thiophilic and the oligonucleotide comprises at least one thiooxonucleobase according to Formula I, wherein X is CH or N, R1 is...
8981076 Synthesis of N-FMOC protected deoxy nucleosides, ribo nucleosides, modified deoxy and ribo nucleosides, and phosphoramidites, and their use in oligonucleotide synthesis  
This invention relates to synthesis of novel -N-FMOC protected nucleosides, succinates, phosphoramidites, corresponding solid supports that are suitable for oligo deoxy nucleosides and RNA...
8969047 Solid support assay systems and methods utilizing non-standard bases  
Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target. In...
8951728 Template directed split and mix synthesis of small molecule libraries  
The invention combines the advantages of split and mix synthesis with the advantages of template directed synthesis. The method comprises the steps of: a) adding a linker molecule L to one or more...
8932992 Templated molecules and methods for using such molecules  
The present invention relates to a method for synthesizing templated molecules. In one aspect of the invention, the templated molecules are linked to the template which templated the synthesis...
8916694 SNPs of apolipoprotein B and modulation of their expression  
Compounds, compositions and methods are provided for modulating the expression of apolipoprotein B. The compositions comprise oligonucleotides, targeted to nucleic acid encoding apolipoprotein B....
8912317 Ribonucleic acid interference molecules of Arabidopsis thaliana  
Sequences of ribonucleic acid interference molecules are provided. For example, in one aspect, at least one nucleic acid molecule comprising at least one of one or more precursor sequences having...
8912142 Method of regulating proliferation and differentiation of keratinocyes  
Provided are methods of regulating keratinocytes proliferation and differentiation by subjecting keratinocytes to an agent capable of modulating activity or expression of IGFBP7, thereby...
8906615 2′-arabino-fluorooligonucleotide N3′→P5′ phosphoramidates: their synthesis and use  
Oligonucleotides with a novel sugar-phosphate backbone containing at least one 2′-arabino-fluoronucleoside and an internucleoside 3′-NH—P(═O)(OR)—O-5′ linkage, where R is a positively charged...
8895723 Methods of preparing substituted nucleotide analogs  
Disclosed herein are methods of preparing a phosphorothioate nucleotide analog, which are useful in treating diseases and/or conditions such as viral infections.
8894946 Sample preparation, processing and analysis systems  
This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In...
8889851 Methods for the synthesis and purification of oligomers  
A reagent for oligonucleotide synthesis or purification, wherein the reagent has a structure of: X—C—L—H (Formula A) wherein X is a phosphoramidite group, an H-phosphonate group, an acetal group,...
8889350 Methods and compositions for isolating polynucleotides  
Methods of isolating target double-stranded polynucleotides with internal single-stranded regions are provided. Compositions and kits comprising double-stranded polynucleotides with internal...
8883994 Switchable nucleic acid aptamer probe and uses thereof in tumor living cell detection and in vivo detection  
A switch mode nucleic acid aptamer probe includes a probe main body, a fluorescence generating unit and a fluorescence quenching unit which are respectively connected to two ends of the probe main...
8883516 Click chemistry on heterogeneous catalysts  
The present invention relates to new methods and reagents for coupling molecules by a Click reaction using a heterogeneous catalyst system. Further, the present invention refers to novel devices...
8883999 Methods and solutions for inhibiting undesired cleaving of labels  
The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of...
8883455 Method for detecting the presence of a nucleic acid in a sample  
An automated method for detecting the presence of a nucleic acid in a sample, where the method is performed within a housing of a self-contained, stand-alone analyzer. The method includes...
8859755 Method for preparing ribonucleoside phosphorothioate  
A method for preparing a phosphorothioate RNA based on the oxazaphospholidine method, wherein cyanoethoxymethyl group is used instead of tert-butyldimethylsilyl group as a protective group of...
8859754 Synthesis of deuterated ribo nucleosides, N-protected phosphoramidites, and oligonucleotides  
The present invention is directed towards the synthesis of high purity deuterated sugars, deuterated phosphoramidites, deuterated nucleobases, deuterated nucleosides, deuterated oligonucleotides,...
8846885 Oligonucleotide with protected base  
The present invention provides a protected nucleotide for elongation, which can be purified efficiently and in a high yield by a liquid-liquid extraction operation, and can achieve an...
8846896 Methods of preparing substituted nucleotide analogs  
Disclosed herein are methods of preparing a phosphorothioate nucleotide analog, which are useful in treating diseases and/or conditions such as viral infections.
8846883 Oligonucleotide ligation  
Oligonucleotide chemistry is central to the advancement of core technologies such as DNA sequencing, forensic and genetic analysis and has impacted greatly on the discipline of molecular biology....
8822671 2'-O-modified RNA  
A 2′-modified ribonucleoside having an alkoxymethyl protective group can be imparted with a high duplex-forming ability by introducing, as a substituent, a halogen atom into the protective group...
8822670 Method of synthesizing cDNA and method of synthesizing RNA chains, and nucleotide-immobilized carrier  
A method of synthesizing a cDNA chain using an insoluble carrier having on the surface thereof a polymer substance containing a first unit having a group derived from a phosphoric ester composing...
8816062 Maleimide-furanyl compounds that can be used in a general method for preparing maleimide-oligonucleotide derivatives  
The compounds of formula (I) substantially in exo form or salts thereof, wherein: X is a biradical selected from —(CH2)n—*, —(CH2CH2O)nCH2CH2—*, methylcyclohexyl and methylphenyl; n is an integer...
8809020 Method enabling the use of extracellular ribonucleic acid (RNA) extracted from plasma or serum to detect, monitor or evaluate cancer or premalignant conditions  
This invention relates to the use of tumor-derived or associated extracellular ribonucleic acid (RNA) found circulating in blood plasma or serum fraction for the detection, monitoring, or...
8809518 Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers  
The present invention relates to a linker or population of linkers that include an oligonucleotide fixed portion and an oligonucleotide variable portion represented by formula (N)n, wherein N is...
8808986 Methods and devices for high fidelity polynucleotide synthesis  
Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments,...
8808995 Methods for genotyping selected polymorphism  
Methods for genotyping polymorphisms using a locus specific primer that is complementary to a region near a selected polymorphism are described. Methods for synthesizing pools of locus specific...
8802745 Porous resin bead and method for producing nucleic acid using the same  
The present invention relates to a porous resin bead containing a first aromatic monovinyl compound-divinyl compound-(meth)acrylonitrile-second aromatic monovinyl compound copolymer, in which the...
8796432 Chemically cleavable 3'-o-allyl-DNTP-allyl-fluorophore fluorescent nucleotide analogues and related methods  
This invention provides a nucleotide analogue comprising (i) a base selected from the group consisting of adenine, guanine, cytosine, thymine and uracil, (ii) a deoxyribose, (iii) an allyl moiety...
8785619 Nucleosides and oligonucleotides for studies on reversal of cytotoxic and mutagenic damage of DNA potential diagnostics tools  
This invention relates to n-alkylated synthetic nucleosides and phosphoramidites of high regio-specific purity and stability and for selective deprotection of the protecting group in...
8772473 Mostly natural DNA sequencing by synthesis  
The invention provides a new method for DNA sequencing called “natural sequencing by synthesis” (nSBS). According to the method, DNA that includes a desired sequence is synthesized using a dNTP...
8765369 Ultrasensitive detection of target using target-ready particles  
The invention relates to methods and reagents for detecting minute amounts of targets having affinity for nucleic acid. The present invention more particularly relates to target detection using...
8765419 Separation of pyrophosphate release and pyrophosphate detection  
The present technology relates to methods and systems for detection of pyrophosphate. As such, disclosed herein are methods and systems that permit improved pyrophosphate detection. Also disclosed...
8765933 Asynthesis of β-nucleosides  
A process of stereoselectively synthesizing β-nucleoside, e.g., 2′-deoxy-2,2′-difluorocytidine, is described. The process includes reacting a tetrahydrofuran compound of the following formula: in...
8759509 Way of using thermolabile groups to protect hydroxyl functions and compounds for implementing the procedure  
A procedure for using thermolabile groups to protect a hydroxyl function, above all in nucleosides, nucleotides, oligomers, nucleic acids during the reactions of organic synthesis. Various new...
8748134 Transcription activator-like effector assembly  
Described herein are techniques for assembling a polynucleotide encoding a transcription activator-like effector nucleases (TALEN). The techniques ligate and digest necessary modules for a TALEN...
8741606 Method of tagging using a split DBR  
Aspects of the present invention include methods and compositions for determining the number of individual polynucleotide molecules originating from the same genomic region of the same original...
8735569 Nucleoside phosphoramidates  
Disclosed herein are nucleoside phosphoramidates and their use as agents for treating viral diseases. These compounds are inhibitors of RNA-dependent RNA viral replication and are useful as...
8729251 Parallel preparation of high fidelity probes in an array format  
The present invention provides massively parallel oligonucleotide synthesis and purification for applications that utilize large collections of defined high-fidelity oligonucleotides (e.g., from...