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5043289 |
Method and device for assaying immunologically reactive substances of clinical interest
A method and device of quantitatively assaying an immunologically reactive substance of clinical interest, wherein the method includes the steps of grafting an immunologically active substance onto...
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5037649 |
Method for treatment of HIV-infected patients
Patients suffering from HIV-1 infection, including both those who have and those who have not developed acquired immunodeficiency syndrome, are treated by extracorporeal removal of IgG and immune...
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5017472 |
Microflotation devices used for immunoassays and cell/molecular fractionation
This invention relates to a flotation immunoassay employing a novel buoyant matrix to which an antigen or antibody is coupled and which separates the bound and free products of the assay by...
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5000960 |
Protein coupling to lipid vesicles
A new method of coupling proteins and other targeting molecules to lipid vesicles has been developed. A bifunctional agent forms a covalent bond without damaging the lipid structure and permits...
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4960715 |
Diagnostic test methods
Agglutination assays, particularly latex agglutination assays, for simultaneous testing for a multiplicity of ligands. The reagents for use in the assays comprise two or more insoluble colored...
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4954452 |
Non-metal colloidal particle immunoassay
A method of performing a diagnostic immunoassay utilizing colloidal non-metal particles having conjugated thereto a binding component capable of specifically recognizing an analyte to be...
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4952520 |
Immunoassay making use of latex agglutination
An immunoassay making use of latex agglutination is practized by providing a colored latex as a latex, subjecting the colored latex to an immune reaction to form agglutinated particles of the...
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4952517 |
Positive step immunoassay
An assay procedure that is particularly valuable for detecting and/or determining the presence of threshold levels of analyte ligands in biological fluids. In one particularized and specialized...
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4943522 |
Lateral flow, non-bibulous membrane assay protocols
A method and apparatus for conducting specific binding pair assays, such as immunoassays, is described. A porous membrane capable of non-bibulous lateral flow is used as assay substrate; a member...
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4880731 |
Process and reagent for the determination of a reaction partner of an immunological reaction
For the determination of a reaction partner of an immunological reaction according to the principle of immunoassay, the reaction partner to be determined is brought into contact with a marked...
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4871661 |
Process for testing the carcinogenicity of a material or the presence of cancer-inducing factors in an environment
Materials can be screened for carcinogenic properties by administering them to test animals and assaying biological tissue, preferably plasma, for the presence of a 60K cancer-associated...
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4868131 |
Competitive binding immunoassay process
An immunoassay process for quantitative analysis of an analyte antigen (or analyte antibody) in an aqueous liquid sample. In the first step, the analyte antigen (or analyte antibody) is allowed to...
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4859612 |
Metal sol capture immunoassay procedure, kit for use therewith and captured metal containing composite
Antibody coated gold sol particles and antibody coated solid phase particles dispersed in an aqueous system react immunologically as a function of the presence of an analyte in a sample to be...
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4853335 |
Colloidal gold particle concentration immunoassay
A sandwich immunoassay method is disclosed that is useful for the identification of an antigen in biological specimens. The method is comprised of a colloidal gold labeled binder specific for...
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4851329 |
Immunoassay employing optical pulse particle size analysis
This invention provides a means for determining the concentration of any of a wide range of antibody or antigen molecules with a high degree of specificity, accuracy and sensitivity. Antigen or...
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4837145 |
Layered immunoassay using antibodies bound to transport particles to determine the presence of an antigen
A device is provided for determining the presence of an antigen which comprises a trapping zone, which contains material capable of capturing free flowing enzyme linked antibodies, but not...
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4824777 |
Method for determining thyroxine uptake
A method for determining thyroxine uptake of a sample serum comprising: (a) incubating the sample serum with solid phase thyroxine and labeled anti-TBG antibody; (b) separating the solid phase from...
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4801449 |
Method for treatment of Kaposi's sarcoma
An immunoadsorbent material for removing IgG and IgG-complexes from biological fluids is prepared by covalently binding protein A to a solid-phase silica matrix. It has been found that particularly...
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4752568 |
Labeled hydantoin conjugate and its use in analytical element and immunoassays
A heterogeneous, competitive binding immunoassay for either phenytoin or phenobarbital is conducted with a labeled conjugate comprising a derivative of hydantoin and a label. The hydantoin...
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4745073 |
Manual immunoassays and apparatus therefor
A cap for a container in which immunoassays are performed includes a liquid absorbing material accessible to liquid in the container.
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4721681 |
Immunoassay in centrifugal field with complementary particles of differing specific gravities
Competitive or inhibition assays are disclosed in which sample (e.g., containing target antigen), a reagent containing first particles (e.g., antibody-coated light particles) and a reagent...
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4720465 |
Centrifugal kinetic agglutination assay
A sample is mixed with a reagent containing stably suspended particles coated with a binding pair member complementary to or competitive with the target binding pair member. A centrifugal force...
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4716108 |
Assaying anti-TP IgM antibodies for syphilis diagnosis
A method of measuring an infectious disease antibody such as syphilis IgM, which comprises treating immunoglobulins of a sample with an anti immunoglobulin antibody sensitized on carrier particles...
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4680274 |
Particles for inhibiting non-specific immunoreaction
In an immunoassay, ultrafine particles having an average particle size of 0.2 μm or smaller and sensitized with a substance, which has reactivity with the materials to induce a nonspecific...
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4665020 |
Flow cytometer measurement of binding assays
A method of measuring the result of a binding assay that does not require separation of fluorescent smaller particles is disclosed. In a competitive binding assay the smaller fluorescent particles...
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4663278 |
Agglutination dependent enzyme channeling immunoassay
A novel agglutination assay is provided which brings together through the intermediacy of the binding of specific binding pair members particles which differ by having different members of a signal...
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4663277 |
Virus detection method and materials
Viruses are detected by means of an immunoassay method in which an extended solid phase coated with antiviral antibody is employed to bind and remove virions from a specimen by forming an...
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4652533 |
Method of solid phase immunoassay incorporating a luminescent label
A method is provided for solid phase immunoassay for quantitation of antigen, hapten or antibody analyte in liquid sample and alternatively for quantitation of analyte occuring on or attached to...
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4650770 |
Energy absorbing particle quenching in light emitting competitive protein binding assays
Assays are provided employing particles and absorbent particles, wherein the absorbent particles substantially inhibit fluorescence when bound to the fluorescent particles through specific...
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4639419 |
Immunological color change test involving two differently colored reagent spots
A method and device for detecting an antigenic material in which the device comprises a test utensil having an indentation in which two reagent spots are placed, the first body being a dyed...
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4623618 |
Simultaneous quantitative immunoassay for different antigens or antibodies
An immunoassay by which a plurality of kinds of antigens or antibodies in one test sample may be simultaneously quantified. Each of a plurality of kinds of microcapsules is first provided for each...
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4590169 |
Direct particle agglutination immunoassays avoiding false negatives at high antigen concentrations
A direct particle agglutination immunoassay for assaying an antigenic substance (Ag) in a fluid. The immunoassay is the type which comprises: (a) contacting the fluid with an antibody (Ab)...
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4588681 |
Process for producing adult T cell leukemia associated antigen
A process for producing an adult T cell leukemia associated antigen is disclosed wherein an adult T cell leukemia associated antigen producing cell is treated with a surfactant. A method for...
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4582792 |
Immunoassay method using two immobilized biologically active substances
A biologically active composition comprising an immobilized phase comprising an antigen or an antibody, and an immobilized phase comprising an enzyme, an enzyme inhibitor or activator, and a method...
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4511662 |
Simultaneous assay for T and B lymphocyte populations and subpopulations
An assay for simultaneously determining the ratio of B cells and T cells to the total cell population and subpopulations thereof present in a lymphocyte population utilizes excess amounts of B cell...
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4486530 |
Immunometric assays using monoclonal antibodies
"Two-site" or "sandwich" immunometric assay techniques for determination of the presence and/or concentration of antigenic substances in fluids using monoclonal antibodies are described and...
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4459361 |
Ligand assay with one or two particulate reagents and filter
Method of making and using products for analysis of specific ligands in solution. More specifically, a method of analyzing a sample for a ligand by incubating the sample with a ligand selective...
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4451434 |
Transparent optical container for non-destructive biological fluid assay
A concept and various means of immunological assay are disclosed wherein two different classes of particles which interact at short distances to produce characteristic detectable signals are...
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4308026 |
Agglutination inhibition immunoassay for hapten using two differently sensitized particles
An immunochemical process and the reagents for measuring hapten by using an agglutination inhibition reaction, anti-hapten antibody sensitized particles and a hapten-carrier conjugate which is...
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4305925 |
Latex reagent
What is disclosed is an improved nephelometric method for detecting an antigen or antibody by contact thereof in a liquid medium with particles of a latex reagent comprising latex particles of...
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4279617 |
Iummunoassay involving agglutination
A particle agglutination assay for antigens, antibodies and other binding proteins such as rheumatoid factor, uses two different, microscopic or submicroscopic particulate reagents. The first...
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4265873 |
Method for typing human leukocyte antigens
A cellular method for typing lymphocyte defined human leukocyte antigens by utilizing human blood leukocytes which have been sensitized to proliferatively respond to the secondary stimulus of the...
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4235792 |
Immunological materials
Immune lysis of sensitized liposomes containing a marker is employed in an assay method wherein an antigenic material to be determined is reacted with a specific antiserum and the degree of...
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4223005 |
Antibody coated bacteria
Process for identification and enumeration of B and T lymphocytes in an unseparated peripheral body sample comprises contacting the sample with two morphologically distinguishable bacteria, each of...
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4191739 |
Antigen-antibody reaction assay employing particle aggregation and resistive pulse analysis
In a mixture of two antibody-coated latex particle suspensions, the particles in each suspension being of a different size, respectively, absence of antigen specific to the antibody in the mixture...
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4184849 |
Mixed agglutination
The presence of antibodies (Ab) and antigens (Ag) in a liquid is detected by mixing the liquid with two different particulate reagents which mutually agglutinate but whose agglutination is...
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4115535 |
Diagnostic method employing a mixture of normally separable protein-coated particles
A mixture of two different kinds of particles having distinctive, different properties is employed for determining the presence of a select protein in, or the absence of a select protein from, a...
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4038378 |
Radioimmunoassay for hepatitis B antigen
The presence of hepatitis B antigen in serum or plasma can be detected by this technique. Commercially available imitation or cultured pearls coated with antibody to hepatitis B antigen are first...
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3917527 |
Hydrophobic chromatography
Biomolecules such as peptides, vitamins, hormones, lipids, proteins and enzymes as well as membranes, cells and the like are separated and purified using one or more members of a homologous series...
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3675768 |
METHOD AND APPARATUS FOR CLASSIFYING AND SEGREGATING PARTICLES WITH ELECTRICAL AND OPTICAL MEANS
Apparatus for classifying, counting and segregating microscopic particles such as cells. The particles may be contained in a gas or liquid and may be classified by measuring two characteristics...
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