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7148016 |
Immunoassays to detect diseases or disease susceptibility traits
Disclosed are immunoassay methods for the diagnosis/prognosis of diseases and disease susceptibility traits associated with gene mutations that cause protein truncation or allelic loss. The levels...
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7056747 |
Process for removing endogenous Vitamin B12 and folate from albumin preparations
Processes for removing endogenous Vitamin B12 and folate are provided. The albumin preparations produced by the process are suitable for use as reference control samples in assays for determining...
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7005426 |
Folic acid-polysaccharide complex, its preparation method and pharmaceutical composition containing the same as active component
The present invention relates to folic acid-polysaccharide complexs and method of preparation thereof, more particularly relates to folic acid-Dextran complexs, method of preparation thereof,...
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6797521 |
Fluorescent cobalamins and uses thereof
The present invention relates to fluorescent cobalamins and uses of these compounds. More particularly, this invention relates to fluorescent cobalamins that comprise a fluorescent, phosphorescent,...
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6555388 |
Binding protein capture assay
This invention relates to binding protein assays. In particular, this invention relates to binding protein assays for B12 and folate in serum or plasma. More specifically, this invention provides a...
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6083926 |
Water soluble vitamin B.sub.12 receptor modulating agents and methods related thereto
Vitamin B 12 receptor modulating agents capable of modulating cell surface receptors by affecting the cell surface receptor trafficking pathway are disclosed. The vitamin B 12 receptor modulating...
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6080549 |
Methods and materials for the diagnosis and treatment of schizophrenia and related disorders
Methods for detecting schizophrenia or depression based on modifications of the contribution of the D 4 receptor to phospholipid methylation levels are described herein. Individuals with...
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5861319 |
Immobilization of specific binding assay reagents
Methods and compositions are provided for specific binding assays in which specific binding reagents are immobilized on a solid phase. Immobilization is facilitated by covalently coupling specific...
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5589327 |
Method and composition for the determination of red blood cell floate
A composition is described which is useful in the assay of folate from a hemolysate prepared from red blood cells using non-radiolabeled assay techniques such as fluorometric, colorimetric,...
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5538901 |
Nucleophilic polysubstituted aryl acridinium ester conjugates uses thereof
This invention is directed to the novel assay methods utilizing nucleophilic polysubstituted aryl acridinium ester conjugates as the tracers. Conjugates prepared by covalent coupling of novel...
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5506109 |
Vitamin B12 assay
Immunoassays for vitamin B 12 using novel monoclonal antibodies to the intrinsic factor:vitamin B 12 complex and to the vitamin B 12 binding site on intrinsic factor.
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5434087 |
Folate immunoassay utilizing folate binding protein in a multiclonal antibody format
An improved method for performing immunoassays whereby specific binding proteins for vitamin B12, folate and other target analytes are utilized with antibodies with different specificities for the...
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RE34394 |
Method and composition for double receptor, specific binding assays
The performance of double receptor, specific binding assays is improved by use of a receptor complex having the structure A BL (BL) n A 1 wherein BL is a binding ligand, A BL is a receptor...
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5227311 |
Intrinsic factor to determine B12
A method for purifying an aqueous intrinsic factor solution which contains R-protein is disclosed. The method involves adding to the intrinsic factor solution an amount of colloidal silica to...
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5187107 |
B.sub.12 enzyme imunoassay and sample pretreatment
Denaturing agents used to pretreat test samples for immunoassays are neutralized and deactivated by the addition of a treatment agent which is a combination of a buffering agent in acid form and an...
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5104815 |
Assay for cobalamins
The current invention is a method, kit and reagents for detecting cobalamins in a sample. The method involves introducing into the sample a first conjugate of a latex or latex-like solid phase...
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5032503 |
Liquid single reagent for air enzyme complementation assay
A method is provided for combining normally interacting reagents in a liquid single reagent and preventing complex formation using a surfactant and then reversing the inhibition by adding a...
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5001072 |
Compositions and methods for multiple simultaneous immunoradiometric assay (IRMA) of analytes using radioisotope chelate labels
Compositions and methods are disclosed for multiple simultaneous assays of different analytes using radioactive labeled antibodies to the analytes, at least one portion of the assay being an...
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4950612 |
Peroxy acid pretreatment in vitamin B.sub.12 assay
Serum samples for vitamin B 12 analysis can be pretreated using a combination of peroxy acid and dithiothreitol. The pretreatment makes vitamin B 12 bound to serum binding proteins available for...
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4918005 |
Method of quantitative assay for vitamin B.sub.12 and reagent for assaying vitamin B.sub.12
The present invention is directed to a method of quantitative assay for a vitamin B 12 -containing substance which comprises culturing marine methanol-utilizing bacteria having B 12 auxotrophy in...
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4904583 |
Cascade immunoassay by multiple binding reactions
A method for enzyme immunoassay includes contacting under binding conditions a liquid suspected of containing an analyte, an antianalyte affixed to a solid support and a tracer having an enzyme...
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4839299 |
Assay for the free portion of substances in biological fluids
A biological sample containing an analyte, partly free and partly bound to natural binders, is incubated with a labelled specific binder for the analyte and a derivative of the analyte which does...
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4806493 |
Stabilizers for use in serum folate assays
A serum folate assay wherein the serum folate is stabilized with dimercaptosuccinic acid or thioctic acid.
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4801530 |
Nucleotide hybridization assay for protozoan parasites
The present invention provides a method for detection of protozoan parasites in blood or other specimen from their mammalian hosts. The method comprises nucleic acid hybridization of repetitive...
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4680273 |
Assay for vitamin B12 deficiency
An assay for Vitamin B 12 deficiency by determining for a blood sample that essentially all of the Vitamin B 12 is carried by a combination of TCI and TCIII and/or essentially no Vitamin B 12 is...
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4672028 |
Compositions and method for simultaneous multiple array of analytes using radioisotope chelate labels
Compounds useful in a simultaneous multiple assay for analytes such as steroids, proteins, peptides, carbohydrates or drugs. The compound or compounds are prepared by labelling an individual...
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4474891 |
Mini-iodinated polypeptide hormone tracer and method for its use
This invention relates to a highly-sensitive mini-iodinated radioactive hormone tracer which has both initial low iodine-to-hormone molar ratios and low specific activity. This invention also...
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4465776 |
Monoclonal antibodies to vitamin B.sub.6 and immunoassay method
A continuous hybridoma cell line which secretes recoverable quantities of monoclonal antibodies having specificity against Vitamin B 6 , which antibodies are useful in a method for detecting the...
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4465775 |
Vitamin B.sub.12 and labelled derivatives for such assay
Vitamin B 12 in liquid samples, such as human serum or human plasma, is assayed by a competitive binding technique using intrinsic factor and certain labelled vitamin B 12 derivatives. The...
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4456689 |
Competitive protein binding assay using an organosilane-silica gel separation medium
An improved competitive protein binding assay comprising; incubating an analyte in the presence of labeled analyte and a protein binder suitable for competitive binding activity by the analyte and...
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4451571 |
Preparation of samples for vitamin B12 and/or folate assay and assay
Vitamin B 12 (cobalamin) and/or folate (folic acid) assay techniques can be simplified, by eliminating the step of heating or boiling the to-be-tested sample prior to its assay. This is...
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4427780 |
Method for assaying vitamin-containing compositions for vitamin analogues
Vitamin-containing multicomponent compositions are assayed to determine the extent of conversion of the vitamin to one or more analogues thereof due to interaction between the vitamin and the other...
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4426455 |
Assay of vitamin B12
A method of assaying for vitamin B12 achieves denaturation of serum binding proteins which normally bind the vitamin B12 by treatment with alkali at ambient temperature. The assay is characterized...
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4423154 |
Simultaneous radioassay of folate and vitamin B12
A serum sample is heated at an alkaline pH to release folate and vitamin B 12 from endogenous binders. A simultaneous radioassay for folate and vitamin B 12 is effected by contacting the sample...
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4418151 |
Assay process with non-boiling denaturation
Competitive protein binding radioassay for sera (or cell) vitamin B 12 and/or serum folate (target components, or analytes) or other target components in liquid ambient utilizing a highly alkaline...
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4399228 |
Polate competitive protein binding assay
Improvement in a competitive protein binding assay for N-5-methyltetrahydrofolic acid wherein any folate standard used is N-5-methyltetrahydrofolic acid complexed with folate binder protein. The...
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4371514 |
Radioimmunoassay of pterins and novel pterin derivatives useful therefor
Pterin derivatives are described having the formula (I) ##STR1## wherein R represents a hydroxyphenyl group, a radioiodinated hydroxyphenyl group, a tyraminocarbonyl group, a radioiodinated...
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4355018 |
Assay for vitamin B.sub.12
A highly specific assay for plasma vitamin B 12 levels in humans is disclosed. The assay employs intrinsic factor preparations obtained from mouse or rat stomachs, which intrinsic factors are free...
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4351822 |
Quantitative testing for vitamin B.sub.12
The vitamin B 12 (cobalamin) level of human blood and mammalian tissue is routinely assayed utilizing radioisotope dilution assay (RIDA) techniques. The presence of vitamin "B 12 analogues" in...
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4336185 |
Folic acid derivatives
Novel protein conjugates and radioiodinated derivatives of folic acid and salts, esters and amides useful in the radioimmunoassay (RIA) of body fluids to determine folic acid are disclosed.
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4333918 |
Radioassay for vitamin B.sub.12
The amount of vitamin B 12 in solution in a liquid is determined by mixing a sample of the solution with vitamin B 12 -binding proteins from chicken serum in a pH of about 12.8 to 13.2, and then...
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4332786 |
Method and composition for vitamin B-12 Assay
The binding of vitamin B-12 by natural substances in samples assayed for vitamin B-12 is reduced by heating the test sample in an alkaline buffer comprising thioglycerol and denaturing agent having...
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4314988 |
Folic acid derivatives and process for preparation
Folic acid derivatives, such as radiolabeled pteroyltyrosine, are conveniently synthesized from either pteroic acid or by the direct condensation of 6-formylpterin with p-aminobenzoyltyrosine...
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4300907 |
Serum vitamin B.sub.12 assay and kit therefor
In a vitamin B 12 assay, serum, containing vitamin B 12 bound to endogenous binders therefor, is released from such endogenous binders by a releasing agent comprised of a water miscible organic...
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4298735 |
Folic acid derivatives
Folic acid derivatives, such as radiolabeled pteroyltyrosine, are conveniently synthesized from either pteroic acid or by the direct condensation of 6-formylpterin with p-aminobenzoyltyrosine...
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4279859 |
Simultaneous radioassay of folate and vitamin B.sub.12
A serum sample is heated at an alkaline pH to release folate and vitamin B 12 from endogenous binders. A simultaneous radioassay for folate and vitamin B 12 is effected by contacting the sample...
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4276280 |
Radiolabeled derivatives of folic acid
Derivatives of folic acid wherein the α-carboxyl group of the glutamyl moiety is substituted with a radical which is capable of being radioiodinated, such as, substituted and unsubstituted tyrosyl...
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4273757 |
Determination of transcobalamins
A process and test kit for the quantitative determination of the individual and total unsaturated Vitamin B-12 binding capacities of the three transcobalamins TC-I, TC-II, and TC-III in serum. The...
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4271140 |
Method and composition for double receptor, specific binding assays
The performance of double receptor, specific binding assays is improved by use of a receptor complex having the structure A BL (BL) n A 1 wherein BL is a binding ligand, A BL is a receptor...
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4235867 |
Acrylamide copolymer matrix for radioimmune assay techniques
Disclosed is a polymer matrix based on a copolymer of acrylamide to immobilize antibodies in the radioimmunological determination of hormones and the pharmaceuticals. The specific copolymers are...
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