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4506019 |
Activated polymer container means and assay method employing the same
Novel activated polyamide polymer container or test tube means and a method for the determination of ligand or analyte content in aqueous samples, are provided. The surfaces of the container tubes...
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4493793 |
Soluble immunoassay reagent comprising lectin covalently bonded to reactive component
A lectin is covalently bonded to an immunological conjugate such as an antibody-antigen or its equivalent. Then, the lectin-conjugate is isolated from the reaction product mixture by one of a...
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4489165 |
Chromogenic tracers for use in an assay
A chromogen, in particular, a fluorescent compound, is conjugated to a ligand through an amino acid spacer radical, which includes a free carboxyl group. The tracers are particularly suitable for...
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4481298 |
Pre-precipitated double antibody immunoassay method
An immunoassay process for the detection of an antigen in a sample, which comprises: (a) forming a mixture of the sample with (1) a preformed complex of a primary antibody and a secondary binding...
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4480042 |
Covalently bonded high refractive index particle reagents and their use in light scattering immunoassays
Novel particle reagents for light scattering immunoassays are provided. The particle reagents are high refractive index shell-core polymers covalently bonded to compounds of biological interest or...
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4480041 |
Use of phosphotriester intermediates for preparation of functionalized liposomes
A method of forming an analyte-functionalized liposome for use in immunoassay techniques employs a phosphotriester intermediate of a phospholipid derivatized with the desired analyte in a process...
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4478945 |
Method for evaluating thyroid function
Clinical method for evaulating thyrometabolic function by determination of clinically important thyroxine concentrations and thyroxine binding protein capacities in plasma. The method employs a...
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4477576 |
Antigen assay method and kit
A method of determining the concentration of an antigen in a sample solution comprising (a) coating an antigen-protein conjugate onto a solid matrix, (b) conjugating an enzyme to an antibody...
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4477578 |
Method and apparatus for performing assays
Method and apparatus are provided for carrying out multiple simultaneous transfers of fluid. The method and apparatus are particularly directed toward immunoassays wherein immunologically active...
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4476229 |
Substituted carboxyfluoresceins
This disclosure relates to a method and reagents for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. This disclosure also relates to a novel...
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4476228 |
Determination of unsaturated thyroxine binding protein sites using fluorescence polarization techniques
This invention encompasses a method for measuring unsaturated thyroxine binding protein sites in a sample comprising intermixing with the sample an effective amount of fluorescent labeled tracer...
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4474891 |
Mini-iodinated polypeptide hormone tracer and method for its use
This invention relates to a highly-sensitive mini-iodinated radioactive hormone tracer which has both initial low iodine-to-hormone molar ratios and low specific activity. This invention also...
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4472508 |
Test for detecting and measuring the graves' disease-specific immunoglobulins
An immunoassay for a specific antibody, particularly Graves' disease-specific antibody, in which interfering reactions by the reactive ends of similar antibodies are eliminated by the step of...
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4472498 |
Analysis film and a method of analysis using the same
An analysis film comprises a reagent layer composed of a porous material which contains an antibody but does not substantially contain a complex of an analyte or a labelled antigen with the...
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4469787 |
Immunoassay involving soluble complex of second antibody and labeled binding protein
A method and kit for determining the presence of a polyvalent ligand in an aqueous fluid. The method involves incubating the fluid with an immobilized antibody to the ligand to form an immobilized...
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4468469 |
Substituted phenylacetic acids and salts as TBP blocking agents in iodothyronine immunoassays
An improved immunoassay method, reagent means, and test kit for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or plasma, wherein fenclofenac and related...
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4467030 |
Determination of the thyroxine-binding index in serum
A method for the determination of the thyroxine-binding index in serum, which method comprises mixing the serum sample to be determined with a defined amount of thyroxine and a defined amount of a...
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4456689 |
Competitive protein binding assay using an organosilane-silica gel separation medium
An improved competitive protein binding assay comprising; incubating an analyte in the presence of labeled analyte and a protein binder suitable for competitive binding activity by the analyte and...
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4454231 |
Method and device for mass transfer operation in immunoassays and other applications
The present invention relates to a new technique and device for mass transfer of one or more components from one liquid phase to another liquid phase, involving a physical separation of said two...
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4442218 |
Method of measuring degree of partitioning
A method of measuring the degree of partitioning of a labeled species between free and bound states which involves the use of an insoluble porous monolith having a means for binding a portion of...
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4432907 |
Diamine acid fluorescent chelates
Species-linked diamine triacetic acids of the formula ##STR1## wherein T is an organic species containing at least one amine, hydroxyl, or thio functional group, L is the residue of at least one of...
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4431741 |
Hypothyroid control serum
A hypothyroid control serum for use in assay of thyroid function of serum with a reagent antiserum, said control comprising (a) serum from which triodothyronine and thyroxine have been stripped and...
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4430263 |
Hapten-inhibitor immunoassay
A specific binding assay method employing, as a labeling substance, a reversible trypsin inhibitor for the detection of a hapten. Competition between the hapten to be determined and hapten trysin...
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4426453 |
Derivatives of iodothyronine compounds and their use in an assay for the free iodothyronine compounds
Novel amides of an iodothyronine compound such as tri-iodothyronine (T3) or thyroxine (T4) with an aminoacetic acid compound such as nitrilotriacetic acid (NTA), ethylenediaminetetraacetic acid...
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4410634 |
Method of passively adsorbing immuno-reactive haptens to solid phases
The method comprises covalently binding an immuno-reactive to a selected macromolecular carrier and then contacting the resulting hapten-carrier conjugate at a selected concentration in a liquid...
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4410633 |
Method for the measurement of free thyroxine or 3,5,3'-triiodothyronine in a liquid sample
An immunoassay for the measurement of free thyroid hormone, i.e., thyroxine or 3,5,3'-triiodothyronine, in a liquid sample in which the thyroid hormone is present in both free and combined states,...
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4401764 |
Immunoassays employing labeled reagent and a conjugate having two binding sites
In the immunoassay of antigens in liquids, a reaction mixture is formed containing the liquid under assay, labelled antigen, and a mixed binding reagent which contains an antigen-binding site and a...
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4391795 |
Assay for free thyroid hormone
Sample containing free and bound thyroid hormone; in particular, thyroxine, is contacted with immobilized binder; in particular, supported on a solid support, to bind the free thyroxine. After...
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4390517 |
Method, composition and kit for stabilizing radiolabeled compounds
A method of stabilizing a solution of a radiolabeled compound comprising adding to such solution a compound having a substantially insoluble backbone, to which has been bound a quaternary ammonium...
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4385126 |
Double tagged immunoassay
Immunoassays are performed with two different ligands tagged with two different tagging constituents which are independent of each other and the two tagged ligands are immunologically bound...
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4381291 |
Measurement of free ligands
A method for indirect measurement of the concentration of a free ligand in a liquid sample also containing the ligand bound to endogenous binding material, comprising the steps of performing two...
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4380580 |
Heterogenous chemiluminescent specific binding assay
A heterogeneous specific binding assay which employs a substance having reactant activity, i.e., a reactant, in a chemiluminescent reaction as a labeling substance in the detection of a ligand in a...
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4378428 |
Method for carrying out non-isotopic immunoassays, labeled analytes and kits for use in such assays
A highly sensitive, immunoassay method for determining the amount of an analyte in a sample containing a known analyte in an unknown concentration is provided. Sample; a polypeptide-labeled analog...
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4378344 |
Method and apparatus for performing multiple, simultaneous in vitro diagnostic tests using a solid phase system
A method for performing multiple, simultaneous in vitro diagnostic tests is provided. The method utilizes a solid phase device comprising a receptacle and an insert. The receptacle has one or more...
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4352751 |
Species-linked diamine triacetic acids and their chelates
Species-linked diamine triacetic acids of the formula ##STR1## wherein T is an organic species containing at least one amine, hydroxyl, or thiol functional group, L is the residue of at least one...
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4347058 |
Thyroid polarization fluoroimmunoassay
A polarization fluoroimmunoassay for T 3 or T 4 thyroid hormones where a serum sample is assayed by contacting the sample with a base to denature the thyroxine binding globulins and thereby form...
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4347059 |
T.sub.3 Uptake polarization fluoroimmunoassay
A T 3 uptake polarization fluoroimmunoassay where a sample is assayed by contacting a serum sample with T 3 to form a solution, contacting the solution with a separating agent and incubating the...
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4341865 |
Determination of thyroxine binding globulin
This invention encompasses a method for measuring unsaturated thyroxine binding globulin in serum comprising intermixing with the serum: (i) thyroxine irreversible enzyme inhibitor conjugate...
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4337063 |
Competitive immunoassay using spectral sensitizer label
Method for immunological analysis of a trace component(s) by labelling an antigen or antibody with a marker comprising causing an immune reaction using an antigen or antibody labelled with a...
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4332784 |
Dual isotope assays
Dual isotope assays for thyroid function are performed by carrying out a radio-immunoassay for two of thyroxine (T4), tri-iodothyronine (T3), thyroid stimulating hormone (TSH), and thyroxine...
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4329461 |
Fluorescent thyroid hormone conjugates and their uses
Novel polyhalothyronines or immunogenic mimetic analogs are provided conjugated to a fluorescer compound with the resulting conjugate being highly fluorescent. Specifically, the compounds have at...
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4312854 |
Immunoassay for thyroid stimulating hormone employing florisil adsorbent as separating agent
An improved immunoassay method for determining thyroid stimulating hormone (TSH) in a liquid sample wherein the bound- and free-species of the labelled, preferably radio-labelled, TSH are separated...
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4311690 |
Test set and method for the determination of free hormones
Disclosed is a process for determining the concentration of unbound hormone, e.g., thyroxine, cortisol, or testosterone, in a liquid sample containing hormone and protein capable of binding the...
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4311687 |
Radiometric assay of dialysates
An improved radiometric assay of dialysates from equilibrium dialysis procedures which involves using immobilized antibody specific for a given hapten to separate free hapten (including...
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4310504 |
Dry radioimmunoassay test composition containing stabilized label/antibody mixture
A dry radioimmunoassay test composition comprising a mixture of a radiolabelled form of a ligand, i.e., a hapten or an antigen, an antibody for the ligand, and a heavy metal cation, e.g., cupric...
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4307071 |
Analytical reagent and method
A reagent is disclosed for determining the concentration of a ligand in blood serum. The reagent includes a protenaceous, three-dimensional, serum-insoluble complex which is readily collected by...
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4307070 |
Methods and apparatuses for performing immunoassays
Methods and apparatuses are featured for preconcentrating immunological reactants prior to their contact and reaction to enhance the rate of reaction for separating reacted and unreacted reactants...
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4301139 |
Multilayer column chromatography specific binding assay method, test device and test kit
A specific binding assay method, and a test device and test kit for use therein, for determining a liqand, such as an antigen or antibody, in, or the ligand binding capacity of, a liquid medium,...
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4299812 |
Immunoassay of thyroxine in neonates using dried blood samples
In the immunoassay of thyroxine using dried blood samples spotted on filter paper, the assay in this invention is run at a pH of 9.2 to 10.0, preferably at about 9.5, rather than the pH range of...
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4297337 |
Solid-phase immunoassays using magnetic glass
Solid-phase immunoassay procedures employing magnetic glass or crystal-containing material, or a superparamagnetic material formed of magnetic particles dispersed in a non-magnetic matrix, as...
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