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8026050 |
Methods for modifying an oligonucleotide
The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and ide...
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7989184 |
Endoribonuclease
A polypeptide having a novel endoribonuclease activity; a nucleic acid encoding the polypeptide; recombinant DNA having the nucleic acid therein; a transformant transformed with the recombinant...
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7955819 |
Process for producing sugar chain asparagine derivative
A process for preparing asparagine-linked oligosaccharide derivatives including the steps of: (a) treating a delipidated egg yolk with a protease to obtain a mixture of peptide-linked...
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7947477 |
Emulsion PCR and amplicon capture
Compositions and methods of use are disclosed for clonally amplifying target polynucleotide sequences in solution and attaching the amplicons to a surface by activation of a masked binding moiety....
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7785853 |
Polypeptides having cellobiohydrolase I activity and polynucleotides encoding same
The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to...
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7435563 |
Method for the production of (s)-3-methylamino-1-(thien-2-yl)propan-1-ol
The present invention relates to a process for the preparation of enantiomerically pure (S)-3-methyl-amino-1-(thien-2-yl)propan-1-ol of the formula II-S by obtainment of...
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6872816 |
Nucleic acid detection kits
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to...
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6833260 |
Lactose hydrolysis
The invention features methods and compositions for rapidly and effectively hydrolyzing lactose using recombinant lactic acid bacteria.
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6669963 |
Stable particle in liquid formulations
A stable particle in liquid formulation comprising a discontinuous phase of microparticles is suspended in a continuous phase which is a non-aqueous liquid, preferably biocompatible in which the...
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6607901 |
Alkaline alpha-galactosidase
An enzyme isolated from an organism that metabolizes alpha-galactosyl containing saccharides, comprising an alpha-galactosidase (E.C. 3.2.1.22, alpha-D-galactoside galatohydrolase) with optimal...
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6541226 |
Method for specifically cleaving double-stranded DNA and kit for the method
A method for specifically cleaving a double-stranded DNA. The method comprises forming a three-stranded portion comprising the double-stranded DNA portion including the position to be cleaved or...
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6531294 |
Preparation of pancreatic procarboxypeptidase B, isoforms and muteins thereof and their use
Pancreatic carboxypeptidase B or an isoform or a mutein of carboxypeptidase B may be prepared by (a) expressing a natural or unnatural enzymatically inactive precursor form of carboxypeptidase B in...
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6504024 |
Propargylethoxyamino nucleotide primer extensions
Propargylethoxyamino nucleosides are disclosed having the structure wherein R1 and R2 are —H, lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W1 is —H or —OH...
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6458535 |
Detection of nucleic acids by multiple sequential invasive cleavages 02
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to...
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6451563 |
Method for making linear, covalently closed DNA constructs
A process to obtain linear double-stranded covalently closed DNA “dumbbell” constructs from plasmids by restriction digest, subsequent ligation with hairpin oligodesoxyribonucleotides, opt...
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6391595 |
Transferase and amylase, process for producing the enzymes, use thereof, and gene coding for the same
The invention provides a novel transferase that acts on a saccharide, as a substrate, composed of at least three sugar units wherein at least three glucose residues on the reducing end are linked...
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6368803 |
Detection of nucleic acids by target-catalyzed formation
A method is disclosed for modifying an oligonucleotide, which method has application to the detection of a polynucleotide analyte. An oligonucleotide is reversibly hybridized with a polynucleotide,...
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6110677 |
Oligonucleotide modification, signal amplification, and nucleic acid detection by target-catalyzed product formation
A method is disclosed for modifying an oligonucleotide, which method has application to the detection of a polynucleotide analyte. An oligonucleotide is reversibly hybridized with a polynucleotide,...
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6110462 |
Enzymatic DNA molecules that contain modified nucleotides
The present invention discloses catalytic or enzymatic DNA molecules that contain a modified nucleotide and that are capable of cleaving nucleic acid sequences or molecules, particularly RNA, in a...
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5968786 |
Method for producing labeled single-stranded nucleic acid probes
Disclosed is a method for the introduction of unidirectional deletions in a cloned DNA segment. More specifically, the method comprises providing a recombinant DNA construct comprising a DNA...
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5898031 |
Oligoribonucleotides for cleaving RNA
Oligomeric compounds including oligoribonucleotides and oligoribonucleosides are provided that have subsequences of 2-pentoribofuranosyl nucleosides that activate dsRNase. The oligoribonucleotides...
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5888780 |
Rapid detection and identification of nucleic acid variants
The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5' nucleases and 3' exonucleases, are used to screen for known...
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5879907 |
Artificial gene coding for authentic human serum albumin, use thereof and method
A structural gene coding for authentic human serum albumin, --optionally supplemented by an upstream triplet coding for methionine and optionally extended by a synthetic preprox -leader-coding...
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5871911 |
Method of site-specific nucleic acid cleavage
A method of cleaving a target nucleic acid molecule is disclosed. A cleavage structure is formed that comprises the target nucleic acid and a pilot nucleic acid. A first region of the target...
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5869242 |
Mass spectrometry to assess DNA sequence polymorphisms
A method for determining the presence of polymorphisms, including mutations, in nucleic acids by using mass spectrometry is presented. The method requires amplification of the nucleic acid region...
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5861252 |
Method of analysis or assay for polynucleotides and analyzer or instrument for polynucleotides
A method of analysis or assay for nucleotides comprises: (1) a step of digesting DNA with a restriction enzyme; (2) a step of discriminating a difference in sequences of the DNA fragments obtained...
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5856093 |
Method of determining zygosity by ligation and cleavage
The invention provides a method of nucleic acid sequence analysis based on repeated cycles of ligation to and cleavage of probes at the terminus of a target polynucleotide. At each such cycle one...
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5851770 |
Detection of mismatches by resolvase cleavage using a magnetic bead support
Disclosed is a method for detecting one or more mismatches between a first and a second nucleic acid, the first and second nucleic acids being capable of preferentially hybridizing. The method...
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5851762 |
Genomic mapping method by direct haplotyping using intron sequence analysis
The present invention is an improved genomic mapping method which is able to generate highly informative polymorphic sites throughout the genome. In addition to being highly polymorphic, the sites...
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5849484 |
In vitro assay for inhibitors of the intron self-splicing reaction in Pneumocystis carinii
The present invention pertains to an in vitro method for assaying for an inhibitor of the catalytic Group I self-splicing intron reaction in the nuclear rRNA genes of Pheumocystis carinii which...
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5843669 |
Cleavage of nucleic acid acid using thermostable methoanococcus jannaschii FEN-1 endonucleases
The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Structure-specific nucleases, including 5' nucleases, thermostable FEN-1...
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5831065 |
Kits for DNA sequencing by stepwise ligation and cleavage
The invention provides a method of nucleic acid sequence analysis based on repeated cycles of ligation to and cleavage of probes at the terminus of a target polynucleotide. At each such cycle one...
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5827704 |
Vectors for cloning and modification of DNA fragments
Plasmid vectors are provided which enable for the modification of double-stranded DNA which has blunt or one-base 3' overhanging termini, to include any desired four-base 5' overhang sequence, in...
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5750341 |
DNA sequencing by parallel oligonucleotide extensions
Method and compositions are provided for analyzing nucleic acid sequences based on repeated cycles of duplex extension along a single stranded template. Preferably, such extension starts from a...
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5747327 |
Phospholipase D gene originated from plant
A cloned DNA encoding phospholipase D originated from a plant and a cloned DNA which regulates expression of phospholipase D gene originated from a plant are disclosed.
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5741678 |
Quantitative method for early detection of mutant alleles and diagnostic kits for carrying out the method
There is disclosed a quantitative sensitive method to enable the detection of point mutations at a known site to a diagnostic kit which uses a multi step (for example, four steps) or a single step...
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5719028 |
Cleavase fragment length polymorphism
A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is...
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5714330 |
DNA sequencing by stepwise ligation and cleavage
The invention provides a method of nucleic acid sequence analysis based on repeated cycles of ligation to and cleavage of probes at the terminus of a target polynucleotide. At each such cycle one...
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5698421 |
Ribonucleoprotein particles for cleaving double-stranded DNA and inserting an RNA/DNA molecule into the cleavage site
The present invention provides new methods, employing a nucleotide integrase, for cleaving double-stranded and single stranded DNA substrates at specific sites and for attaching nucleic acid...
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5695971 |
Phage-cosmid hybrid vector, open cos DNA fragments, their method of use, and process of production
A hybrid vector produced from a linearized cosmid and arms from λ phage vector DNA has been developed. The hybrid vector can be used to generate fragments which are useful as vectors in a helper ...
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5695961 |
Bi-functional expression system
A novel expression system is provided comprising a DNA sequence containing transcriptional and translational signals that promote the over production of recombinant proteins both in bacterial hosts...
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5670346 |
Modifying nucleotide analogues
Fluorescently labelled dideoxynucleoside triphosphates are widely used as chain-terminators in Sanger dideoxy sequencing operations. But these unincorporated dye-terminators migrate in an...
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5665545 |
Terminal repeat amplification method
This invention relates to a process for amplifying a specific nucleic acid sequence or its complement at a relatively constant temperature and without serial addition of reagents. The process...
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5641633 |
Fluorescence polarization detection of nucleic acids
The present invention provides methods for detecting amplified or unamplified nucleic acid target sequences at increased temperatures by changes in fluorescence polarization. The decrease in...
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5580759 |
Construction of recombinant DNA by exonuclease recession
An exonuclease-based method for joining and/or constructing two or more DNA molecules. DNA fragments containing ends complementary to those of a vector or another independent molecule were...
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5578448 |
Nucleic acids encoding wild-type measles virus consensus hemagglutinin and fusion polypeptides and methods of detection
The present invention provides nucleic acids encoding consensus hemagglutinin and consensus fusion polypeptides which contain at least one amino acid substitution relative to the Moraten vaccine...
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5573912 |
Process of selecting and/or obtaining probes capable of detecting new variable number tandem repeat regions
According to the process of the invention, sets of restriction fragments from the DNA of an individual are prepared, these restriction fragments being separated by size. Probes are prepared by...
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5554516 |
Nucleic acid sequence amplification method, composition and kit
A method, composition and kit for amplifying a target nucleic acid sequence under conditions of substantially constant temperature, ionic strength, and pH and using only a single promoter-primer....
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5516664 |
Enzymatic synthesis of repeat regions of oligonucleotides
Enzymatic synthesis of a repeat region of an oligonucleotide may be performed by the steps of: (a) combining a primer and a blocked nucleotide in the presence of a chain extending enzyme whereby a...
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5484701 |
Method for sequencing DNA using biotin-strepavidin conjugates to facilitate the purification of primer extension products
A simplified method for isolating primer extension products and generating them in a form appropriate for electrophoresis is disclosed. The method is compatible with automated DNA sequencing...
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