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5068189 |
Recombinant DNA vectors encoding a 4"-O-isovaleryl acylase derived from a carbomycin biosynthetic gene, designated carE, for use in streptomyces and other organisms
The carE gene of Streptomyces thermotolerans has been isolated and used to construct recombinant DNA expression vectors. The carE gene encodes 4"-O-isovaleryl acylase activity important in the...
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5015575 |
Hybrid DNA synthesis of insulin
Methods and compositions are provided for efficient production of polypeptides having insulin activity. The proinsulin gene is joined to secretion and processing signals for expression and...
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5013658 |
Transposon tagging of genes in transformed plants
This invention relates to improved methods for transposon tagging as a route to plant gene isolation. The specific improvements comprise efficient means for preselecting plants which have undergone...
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4959314 |
Cysteine-depleted muteins of biologically active proteins
Muteins of biologically active proteins such as IFN-β and IL-2 in which cysteine residues that are not essential to biological activity have been deleted or replaced with other amino acids to...
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4885242 |
Genes from pichia histidine pathway and uses thereof
Novel DNA sequences which code for the production of Pichia histidinol dehydrogenase, phosphoribosyl-ATP-cyclohydrase and phosphoribosyl-ATP-pyrophosphohydratase are provided. Novel constructs...
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4830969 |
Process for the rapid and simple isolation of nucleic acids
A process for the separation from other cellular materials of heat agglomeration resistant water soluble nitrogen containing organic compounds such as plasmids, RNA's, mitochondrial DNA's, viral...
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4808537 |
Methanol inducible genes obtained from pichia and methods of use
A method for isolating and cloning methanol inducible genes from Pichia is taught. The regulatory regions are useful for the methanol regulated expression of heterologous genes.
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4784952 |
Conferred susceptibility to lambda phage in non-coliform procaryotic hosts
Vectors suitable for effecting expression of lamB protein in a desired procaryotic host, and methods for their construction, are disclosed. Transformation with these vectors results in the ability...
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4761372 |
Mutant enterotoxin of E. coli
A plasmid which comprises genes coding for an immunogenic, non-toxic, heat-labile enterotoxin and/or a non-toxic, heat-stable enterotoxin are disclosed. The E. coli containing this plasmid is also...
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4740470 |
Biologically functional molecular chimeras
Method and compositions are provided for replication and expression of exogenous genes in microorganisms. Plasmids or virus DNA are cleaved to provide linear DNA having ligatable termini, which are...
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4731327 |
Method for stabilizing recombinant DNA molecules and enhancing the expression thereof in strains of Bacillus
A recombinant DNA molecule, a portion of which includes a vector whose replication is initiated independently of the temperature-sensitive mutant gene of a temperature-sensitive mutant strain of...
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4711848 |
Site specific mutagenesis in alpha-1-antitrypsin
Methods are provided for producing sites specific mutagenized alpha-1-antitrypsin. Particular mutants are formed having a mutation in the active site of alpha-1-antitrypsin at amino acid position...
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4656134 |
Gene amplification in eukaryotic cells
Method and compositions are provided for gene amplification. A DNA unit is prepared capable of replication in a eukaryotic host having tandem genes, where the first gene is capable of complementing...
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4621061 |
Plasmid p SG 2 and process for its preparation
The new streptomyces plasmid p SG 2, having a molecular weight of 9.2 megadaltons, a contour length of 4.58 μm and a molecular length of about 13.8 kilobases, is described, together with its...
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4617267 |
Plasmid pCG1 from Corynebacterium glutamicum
Disclosed is a novel vector, plasmid pCG1, and a process of producing plasmid pCG1 from Corynebacterium glutamicum.
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4582802 |
Stimulation of enzymatic ligation of DNA by high concentrations of nonspecific polymers
DNA ligase activity is strongly accelerated in the presence of high concentrations of non-specific polymers, with an accompanying change in product distribution characteristics. The rate of...
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4518698 |
Plasmid and production thereof
A novel plasmid pATM3 is obtainable from a microorganism belonging to the genus Streptomyces. The plasmid pATM3 is useful as a cloning vector in recombinant DNA work.
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4517300 |
Plasmid pSAN 181
A novel plasmid, named pSAN 181, has a molecular weight of 5±0.2 megadaltons and single cleavage sites for each of the restriction endonucleases BamHI, PstI, XhoI and BglII, the positions of said...
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4506014 |
Plasmid pAC 1, a process for obtaining it and its use
Plasmid pAC 1, which is obtained from Acremonium chrysogenum ATCC 14553 and has a contour length of about 6.7 μm and a molecular size of about 20.9 kilobases (=kb), a process for obtaining it and...
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4495280 |
Cloned high signal strength promoters
Method for preparing high signal strength promoters and terminators and DNA compositions employing such promoters and terminators. T5 phage is cleaved to provide for DNA sequences having intact...
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4489160 |
Plasmid pCG2
Disclosed is a novel vector, plasmid pCG2, and a process of producing plasmid pCG2 from Corynebacterium glutamicum.
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4460688 |
Plasmid and its use
Plasmid conferring resistance to streptomycin (Sm R ) and neomycin (Neo R ) upon its host (e.g. a Bacillus, Staphylococcus or Escherichia coli) and which after having taken up a foreign DNA...
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4418194 |
DNA Fragments for forming plasmids
The present invention relates to novel, broad bacterial host range small plasmid deoxyribonucleic acid rings which serve as cloning vehicles for DNA fragments, particularly those separated from...
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4376164 |
Process for preparing broad host range small plasmid rings as cloning vehicles
The present invention relates to novel, broad bacterial host range small plasmid deoxyribonucleic acid rings which serve as cloning vehicles for DNA fragments, particularly those separated from...
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4273875 |
Plasmid and process of isolating same
A novel chemical compound, essentially pure plasmid pUC6, which is obtainable from a biologically pure culture of the microorganism Streptomyces espinosus biotype 23724a, NRRL 11439. The pUC6...
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4237224 |
Process for producing biologically functional molecular chimeras
Method and compositions are provided for replication and expression of exogenous genes in microorganisms. Plasmids or virus DNA are cleaved to provide linear DNA having ligatable termini to which...
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