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5055397 |
Geomicrobiological methods of ore and petroleum exploration
Geomicrobiological exploration method employing one or more culture, luminometry or tritiated thymidine assays of microbes (bacteria) for sensitivity, determined as survival rate in percentage, of...
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5004684 |
Method for ATP extraction
A method for the extraction of ATP from a microorganism which comprises contacting said microroganism with an ATP releasing agent and thereafter contacting the resultant solution with a...
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4971903 |
Pyrophosphate-based method and apparatus for sequencing nucleic acids
A method for sequencing nucleic acid polymers is provided in which the polymer to be sequenced acts as a template for the production of a complementary polymer by a polymerase enzyme. The template...
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4927752 |
Support used in bioluminescent dosing of enzymes, substrates or enzymatic inhibitors
Method for the bioluminescent dosing of enzymes, substrates or enzymatic inhibitors, characterized in that there is used a transparent support made of synthetic plastic material previously treated...
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4871660 |
Luminescence immuno-test kits, method for stabilizing same and quality control thereof
Luminescence immuno-test kits consist of an antigen or antibody capable of showing luminescence and labelled with phthalic hydrazides, an antibody or antigen preferably bound to a carrier and an...
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4863689 |
Apparatus for the detection of chemiluminescence
Apparatus is provided for the detection of chemiluminescence, e.g., the luminescent assay of an analyte in a sample. Sample, namely the production of a permanent photographic record of such...
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4861709 |
Detection and/or identification of microorganisms in a test sample using bioluminescence or other exogenous genetically-introduced marker
A method for determining the presence of microorganisms in a tests sample. Exogenous DNA containing a luminescent system or other genetic marker system derived from a suitable donor source is...
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4853327 |
Enhanced phthalazinedione chemiluminescence
A chemiluminescence process comprising the contacting of a chemiluminescence precursor, an oxidant, an enzyme and a nitrogen compound selected from the group consisting of ammonia and a...
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4833075 |
Immobilized luciferase for the quantitative determination of ATP
Luciferase is immobilized on a membrane prepared from an absorbent albuminoid such as procine skin gelatin. The membrane may be formed by spreading a solution of porcine skin gelatin on a support...
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4765961 |
Apparatus for detection of certain nitrogen-containing gases using chemiluminescence
Chemiluminescence is detected in a luminol solution free of metal ions flowing through a porous material to indicate the presence of certain nitrogen-containing gases. Continuous monitoring of...
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4761382 |
Immunological procedure for detecting or quantifying substances
This invention relates to a method of detecting, analyzing, quantifying, or locating a substance of biological interest by an immunoassay in which a first component of an immune reaction in the...
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4743561 |
Luminescent assay with a reagent to alter transmitive properties of assay solution
An improved method and reagents are disclosed for determining a ligand in an assay solution containing the ligand, a reagent system and a luminescent compounds, wherein the intensity of the light...
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4713324 |
Inverted latency specific binding assay
A specific binding assay composition and method for determining a ligand in a sample are disclosed. The composition comprises (a) a binding partner for the ligand; (b) a detection system which has...
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4704355 |
Assay utilizing ATP encapsulated within liposome particles
An assay utilizing receptor or antibody sensitized liposome particles which have ATP encapsulated therein. The ATP is released by lysing the liposomes, and detected by means of luciferin-luciferase...
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4687606 |
Metalloid precursor powder and method of making same
A method of making a metalloid precursor powder is disclosed, which powder avoids impurity localization and is effective to produce an improved fine grained ceramic body. A metalloid melt is formed...
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4687747 |
Phenanthridinium ester as a labelling compound in luminometric immunoassay
Disclosed are novel chemiluminescent compounds and their method of use in luminometric immunoassays. The compounds, comprising salts of phenanthridinium esters, are particularly useful in sandwich...
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4665022 |
Bioluminescent assay reagent and method
Reagent for immunoassay, ligand binding assay and ligand receptor assay in which luciferin is covalently bonded to a molecule having biological activity for bonding to a particular biologically...
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4628035 |
Immunoassay method
An immunoassay method for measuring a concentration of an antigen for a short period of time by immobilizing an antibody over the whole zone of an effective supporting matrix for electrophoresis...
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4614712 |
Immunoassays with luciferase labeled ligands or receptors
Immunoassays which utilize an enzyme linked ligand or receptor wherein the enzyme is bacterial luciferase; mercantile kit useful in performing said immunoassay; and compounds utilized in performing...
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4604364 |
Bioluminescent tracer composition and method of use in immunoassays
A tracer composition is described whereby a nonradioactive photon emitter is coupled to a ligand, antigen or antibody for use in various immunoassay methods of analysis. The photon emitters...
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4501813 |
Bioluminescence method for determining NADH or NADPH
A method for determining NADH and/or NADPH-concentrations, for instance in NADH- or NADPH transforming systems, the method comprising the step of bringing the sample subject to determination in...
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4492751 |
Heterogenous specific binding assay employing an enzyme substrate as label
An improved heterogenous specific binding assay method which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium....
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4483929 |
Liposomes with glycolipid-linked antibodies
Lipid vesicles, labelled with encapsulated reporter compositions and bound to antibodies comprise a new class of immunoreagent, useful in immunoassays for ligands.
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4421848 |
Method of detecting the presence of live organisms in substances
The substance to be tested is premixed by gentle agitation in a glass cuvette with an excess of firefly essence and with a buffer solution and cutting agent, so that any live organisms present are...
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4390620 |
Method and composition for the detection and study of cellular activity or the like and means for applying such method
The invention pertains to a method for the study of the behaviour or detection of cells, organites or cellular fractions, possibly present in a medium. It comprises providing said medium with the...
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4385113 |
Rapid, quantitative determination of bacteria in water
A bioluminescent assay for ATP in water borne bacteria is made by adding nitric acid to a water sample with concentrated bacteria to rupture the bacterial cells. The sample is diluted with sterile,...
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4357420 |
Bioluminescence methods for enzymatic determinations
An enzymatic method for continuous, on-line and rapid detection of diagnostically useful biomarkers, which are symptomatic of disease or trauma-related tissue damage, is disclosed. The method is...
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4347058 |
Thyroid polarization fluoroimmunoassay
A polarization fluoroimmunoassay for T 3 or T 4 thyroid hormones where a serum sample is assayed by contacting the sample with a base to denature the thyroxine binding globulins and thereby form...
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4347059 |
T.sub.3 Uptake polarization fluoroimmunoassay
A T 3 uptake polarization fluoroimmunoassay where a sample is assayed by contacting a serum sample with T 3 to form a solution, contacting the solution with a separating agent and incubating the...
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4330620 |
Method and reagent for the determination of creatine kinase MB
A method for the determination of creatine kinase-MB in serum by immunological exclusion of sub-unit M and measurement of the sub-unit B for the determination of creatine kinase, the improvement...
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4318707 |
Macromolecular fluorescent quencher particle in specific receptor assays
Homogeneous immunoassay employing a light absorbing particle to which a member of an immunological pair (mip)-ligand and antiligand is bound and a labeled mip, where the label is light emitting....
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4314026 |
Process for determining the complement-dependent cytotoxicity mediated by anti-HLA antibodies by means of ATP determination and device for ATP determination
The present invention relates to a process for determining the complement-dependent cytotoxicity mediated by anti-HLA antibodies wherein the cytotoxicity is determined by measuring the loss of...
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4303752 |
Selective determination of nucleotides in viable somatic and microbial cells
A method is disclosed for measuring the concentration of nucleotides and the number of viable cells in a biological sample containing somatic and/or microbial cells by treatment of a sample of...
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4302534 |
Chemiluminescent enzyme immunoassay
A heterogeneous enzymatic immunoassay, in which chemiluminescence is employed as a detection means, is disclosed. The chemiluminescence is produced by an enzymatic catalyzed redox reaction between...
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4286057 |
Method and reagent for the determination of creatine kinase
Creatine kinase is determined by the reaction of creatine phosphate with adenosine diphosphate with the formation of adenosine triphosphate, reaction of the latter with luciferin and oxygen in the...
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4283490 |
Method for detection of low level bacterial concentration by luminescence
An improved method for detecting very low concentrations of luminescent reactive molecules from microbial cells in samples of fluids and degraded solids is disclosed. A sample containing microbial...
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4278761 |
Enzyme assay and kit therefor
Compositions and methods featuring, in one aspect, an enzyme assay in which one of a pair of enzymes is immobilized on an insoluble support, and brought into contact with a solution containing the...
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4278760 |
Method and composition for determining an oxidized pyridine co-enzyme
A method for the quantitative determination of an oxidized pyridine co-enzyme which comprises reacting a sample containing oxidized pyridine co-enzyme in the presence of its specific alcohol...
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4277437 |
Kit for carrying out chemically induced fluorescence immunoassay
A competitive protein binding method is provided for the determination of an analyte which is a member of an immunological pair consisting of ligand and receptor for the ligand. A chemiluminescent...
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4264727 |
Assaying of digoxin by means of bioluminescence and inhibition of NA.sup.+ - .sup.+ -ATPase
A method is described for quantitative measurement of digoxin and other digitaloids in serum. The method is based on the inhibition of Na + , K + --specific ATPase enzyme by digitaloids as a...
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4246340 |
Method and reagent for bioluminiscence
An improvement in the process for the determination of the concentration of adenosinetriphosphate (ATP) in ATP-converting reactions wherein the concentration is a function of the intensity of light...
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4238195 |
Fluorescer-labeled specific binding assays
A specific binding assay method and reagent means for determining a ligand, such as an antigen or antibody, in, or the ligand binding capacity of, a liquid medium which employs a fluorescent...
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4235961 |
Method for photometric determination of the subunit B of creatine kinase and a reagent for carrying out the method
A method for the photometric determination of the subunit B of creatine kinase (CK-B) in buffered water solution through reaction with creatine phosphate, ADP and the system luciferase/D-luciferin...
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4234681 |
Immobolized light emitting systems
Light emitting systems are immobilized on a support to provide a localized, concentrated release of light that increases the sensitivity of detection systems when assaying bio-materials. A...
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4220450 |
Chemically induced fluorescence immunoassay
A competitive protein binding method is provided for the determination of an analyte which is a member of an immunological pair consisting of ligand and receptor for the ligand. A chemiluminescent...
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4144134 |
Method for detection of bacterial concentration by luminescence
A method for detecting low levels of microbial cells in samples of fluids and degraded solids. The non-microbial reactive materials in a sample are solubilized by selective use of enzymes and the...
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4132599 |
Determination of antimicrobial susceptibilities on infected urines without isolation
Method for the quick determination of the susceptibilities of various unidentified bacteria contained in an aqueous physiological fluid sample, particularly urine, to one or more antibiotics. A...
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4104126 |
Non-isotopic substrate assay employing bacteriolysis products
Substrates such as haptens and antigens, and those for receptor proteins and native circulating binding proteins are assayed by determining bacteriolysis products occasioned by bacteriophage...
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4080265 |
Method for the determination of creative phosphokinase enzyme
A method for the detection and the quantitative determination of the enzyme creatine phosphokinase (CPK), an agent present in abnormal concentrational levels in serum in many cases of mental...
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4076502 |
Method, composition, and device for determining the specific gravity of a liquid
A method, composition, and device for determining the specific gravity of a liquid. In the method, a sample of the liquid to be tested is contacted with standardized quantities of substances which...
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