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4828989 |
Process for purifying a protein
A process for purifying a protein and particularly the purification of a protein, such as gamma interferon, that forms highly insoluble aggregates during the growth of host cells transformed with...
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4826764 |
Enhancing the translational initiation frequency of a gene
The invention concerns the intercistronic DNA sequence of the atp operon of Escherichia coli for the subunit c of the ATP synthase, a DNA structure and an expression vector which are characterized...
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4820638 |
Novel alpha interferon species produced by recombinant means
A novel species of alpha interferon is taught. The alpha interferon amino acid sequence is disclosed and a gene corresponding thereto. The alpha interferon gene is expressed in Escherichia coli.
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4808523 |
Constitutive production of human IFN-β1 by mammalian cells transformed by the IFN-β1 gene fused to an SV40 early promoter
The invention concerns the production of the human fibroblast interferon, IFN-β1 by cells of mammalian origin which are capable of constitutively expressing a DNA sequence encoding for IFN-β1,...
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4793995 |
Modified (1-56) beta interferons
Modified beta interferons containing amino acid substitutions in the beta interferon amino acids 1 to 56 are described. These modified beta interferons exhibit changes in the antiviral, cell growth...
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4783405 |
Recombinant DNA expression vectors useful in bacillus and other host cells
Recombinant DNA expression vectors for use in Bacillus and other host cells are disclosed. The vectors comprise the veg promoter sequence of Bacillus subtilis, a novel ribosome binding...
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4780530 |
Novel interferon alphas
Novel IFNαS51B10 and IFNαS17H9 of this invention are prepared from BALL-1 cell induced with Sendai virus according to the well known recombinant DNA technique. Further, this invention relates to...
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4766066 |
Method of using bacteriophage lambda p.sub.1 promoter to produce a functional polypeptide in streptomyces
The invention relates to a method and cloning vehicle for the expression of a functional polypeptide in Streptomyces. A recombinant DNA cloning vehicle was genetically engineered to bring the...
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4758512 |
Hosts and methods for producing recombinant products in high yields
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4758513 |
Human T or B cell lines and a process for producing immunologically active substances using the same
This invention relates to a human T or B cell line carrying at least one Ela human adenovirus 12-type gene or fragment thereof; said cell line being capable of spontaneously producing an...
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4758656 |
Novel human interferon-gamma polypeptide derivative
Disclosed is a novel derivative of human interferon-γ polypeptide, a recombinant plasmid wherein a DNA fragment coding for said polypeptide is incorporated and a process for producing the...
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4758428 |
Multiclass hybrid interferons
New multiclass hybrid interferon polypeptides, their corresponding encoding recombinant DNA molecules and transformed hosts which produce the new interferons are described. The amino acid sequences...
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4752577 |
Method for using a novel transcriptional- and translational-activating sequence in streptomyces
The present invention is a method for expressing functional polypeptides in Streptomyces using a recombinant DNA expression vector comprising a novel transcriptional- and translational- activating...
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4751078 |
Process for the purification of gamma interferon
A process is disclosed for purifying gamma interferon from various contaminants resulting from disruption of the cell in which the interferon was produced. The process provides for sequential...
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4748233 |
Alpha-interferon Gx-1
A cloned human alpha-interferon Gx-1 gene, plasmids containing the human alpha-interferon Gx-1 gene, and microorganisms transformed by those plasmids are disclosed. Also disclosed is the...
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4746608 |
Process for producing peptides
A peptide can be produced in a high yield by culturing a microorganism containing a recombinant DNA comprising a eukaryotic gene coding for the peptide, a vector and a promoter which is capable of...
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4745057 |
Method, vectors and transformants for high expression of heterologous polypeptides in yeast
Disclosed are a novel method for inducing the high expression of a nucleotide sequence which is under the transcriptional and translational control of the yeast YG100 gene and the novel vectors,...
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4738931 |
Human interferon-β gene
The present invention relates to human interferon-β gene derived from human chromosome, a DNA containing said gene and a DNA responsible for control of its transcription and a recombinant DNA of...
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4734491 |
DNA sequences encoding hybrid lymphoblastoid-leukocyte human interferons
DNA sequences encoding for novel human lymphoblastoid-leukocyte hybrid interferons. Methods of making nucleotide sequences comprising ribosome binding sites, promoter regions, and segments of human...
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4722897 |
Viral enhancer DNA segments
An enhancer DNA segment having an excellent action of enhancing the transcriptional efficiency of an incorporated gene in a cell, said segment being derived from a human papovavirus BK mutant...
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4716112 |
Vectors for increased expression of cloned genes
Improved expression vectors for amplifying expression of DNA sequence encoding a desired polypeptide beyond that expected by the vector copy number. The vector contains a DNA sequence within the...
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4716217 |
Hybrid lymphoblastoid-leukocyte human interferons
DNA sequences encoding for novel human lymphoblastoid-leukocyte hybrid interferons. Methods of making nucleotide sequences comprising ribosome binding sites, promoter regions, and segments of human...
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4714611 |
Stable composition of gamma-interferon
A stable human γ-interferon composition is produced by adding dextran and/or hydroxyethylstarch to an aqueous human γ-interferon solution freezing the resulting solution and, if desired, drying...
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4705750 |
Promoter plasmid containing the promoter and use thereof in transforming Bacillus
A novel recombinant DNA containing the base sequence shown in FIG. 1 or a portion thereof which exhibits promoter activity. The base sequence exhibiting a potent promoter activity is obtained from...
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4686191 |
Recombinant plasmid containing human interferon-beta gene
Recombinant vector plasmids containing a DNA fragment coding for human interferon-β inserted downstream from a tryptophan promoter are useful for transformation of microorganisms such as...
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4681930 |
Immune interferon and a method for its extraction and purification
An intact human immune interferon protein and a method for the extraction and purification of intact recombinant human immune interferon is disclosed. This method permits the purification to...
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4680262 |
Periplasmic protein recovery
Periplasmic proteins are recovered from transformed gram negative bacteria by a process comprising freezing and thawing the cells. Advantages are obtained by culturing the cells in...
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4680260 |
Method for producing human leukocyte interferon alpha-2
A method for producing human leukocyte interferon alpha-2 resides in that there is carried out submerged cultivation of a producer strain Pseudomonas species VG-84 carrying a plasmid pVG3 with an...
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4678751 |
Hybrid human leukocyte interferons
Disclosed herein are methods and means of microbially preparing novel human hybrid leukocyte interferons, useful in the treatment of viral and neoplastic diseases, by DNA recombination of parental...
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4663280 |
Expression and secretion vectors and method of constructing vectors
The present invention relates to an expression and secretion vector which comprises the signal and promoter sequence of the Bacillus cereus (herein "B. cereus") gene which codes for penicillinase...
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4663285 |
Chimeric plasmids
A plasmid selected from (a) a plasmid conferring resistance to tetracyline (Tc r ) and neomycin (Neo r ) on a host, and being built up by the in vitro ligation of a Neo r non-chimeric plasmid...
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4659570 |
Preparation containing stabilized physiologically active substance
A preparation of stabilized physiologically active substance which is produced by incorporating a physically and chemically modified gelatin in a physiologically active substance made of basic...
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4656131 |
Method for producing interferons
A method for producing high yields of interferons which comprises cultivating a microorganism carrying an expression vector with a structural gene of interferon inserted therein in a chemically...
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4634678 |
Plasmid cloning and expression vectors for use in microorganisms
DNA cloning and expression vectors capable of replication in a microbial host comprising from upstream to downstream (a) at least one promoter; (b) a translation start codon; (c) a cloning segment...
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4631259 |
Transposon in cloning DNA
Processes and new genetic materials are provided for cloning specific DNA fragments by using a unique conjugative transposon designated Tn916. The transposon is used to first target specific genes...
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4620948 |
Purification and activity assurance of precipitated heterologous proteins
A process for isolating and purifying refractile proteins from host cell culture including treating the host cell culture with a buffered solution to solubilize host protein, leaving refractile...
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4615974 |
Yeast expression vectors
There are described a number of plasmid vectors suitable for the expression of genetic material, at various levels in yeasts. The plasmids each comprise a yeast selective marker, a yeast...
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4604284 |
Homogeneous immune interferon fragment
An active fragment of human immune interferon and a method for the extraction and purification of this protein fragment is disclosed. This method permits the purification to homogenity of the...
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4590163 |
Broad host range DNA cloning system for gram-negative bacteria
Novel compositions and methods for cloning DNA in a broad range of Gram-negative bacteria. Relatively small vectors are prepared substantially free of DNA sequences coding for transfer functions,...
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4588585 |
Human recombinant cysteine depleted interferon-β muteins
Muteins of biologically active proteins such as IFN-β and IL-2 in which cysteine residues that are not essential to biological activity have been deleted or replaced with other amino acids to...
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4582800 |
Novel vectors and method for controlling interferon expression
Improved vectors and methods for regulating the expression in bacteria of a eucaryotic anti-viral protein, such as mature human immune interferon, the gene for which has been cloned onto a...
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4568640 |
Method of inserting amino acid analogs into proteins
A method for substituting one amino acid for another in a protein chain to improve selected properties of the protein. Initially, an mRNA molecule capable of producing an unmodified protein of the...
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4559300 |
Method for using an homologous bacillus promoter and associated natural or modified ribosome binding site-containing DNA sequence in streptomyces
A method for expressing a functional polypeptide in Streptomyces comprises transforming a Streptomyces host cell with a recombinant DNA expression vector and then culturing the transformed cell...
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4559302 |
DNA for directing transcription and expression of structural genes
The present invention relates to a novel transcriptional and translational activating sequence. The novel activating sequence can be either chemically synthesized or isolated on a 0.17 kb PstI-SacI...
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4530901 |
Recombinant DNA molecules and their use in producing human interferon-like polypeptides
Recombinant DNA molecules and hosts transformed with them which produce polypeptides displaying a biological or immunological activity of human interferon, the gene coding for these polypeptides...
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4530787 |
Controlled oxidation of microbially produced cysteine-containing proteins
Method of oxidizing reduced cysteine-containing microbially produced synthetic proteins, such as synthetic IFN-β or synthetic IL-2, in a controlled manner so that the synthetic proteins have the...
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4518526 |
Purification and activity assurance of precipitated heterologous proteins
A process for isolating and purifying a precipitated heterologous protein from a host cell culture, including treating the host cell culture with a buffered solution of ionic strength suitable to...
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4518690 |
DNA promoter sequence of Avian tumor virus and use thereof for enhanced gene expression in E. coli
A molecule of double-stranded DNA having a length of about 35 base pairs has been removed from the first 150 base pairs at the 3'-end of Avian sarcoma viral DNA. This molecule binds to E. coli RNA...
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4506013 |
Stabilizing and selecting recombinant DNA host cells
A method for stabilizing and selecting host cells containing recombinant DNA which expresses a functional polypeptide and the novel organisms and cloning vectors for the practice thereof. The...
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4503035 |
Protein purification process and product
An improved process for purifying proteins, particularly proteins having molecular weights in excess of 12,000 involves novel applications of high performance liquid chromatography on a preparative...
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