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5639633 Method for synthesizing peptides with saccharide linked enzyme polymer conjugates  
A method is disclosed for synthesizing peptides using water soluble enzyme polymer conjugates. The method comprises catalyzing the peptide synthesis with enzyme which has been covalently bonded to...
5639859 Carboxylate protective groups, a process for their preparation, their coupling to a functional group, and their use  
Carboxylate protective groups, a process for their preparation, their coupling to a funcitonal group, and their useCarboxylates of polar, hydrophilic alcohols of the formula ##STR1## in which R is...
5629173 Methods of use of serine protease variants having peptide ligase activity  
The invention relates to serine protease variants derived from precursor serine proteases via recombinant and/or chemical methods to form protease variants having improved peptide ligase activity....
5618689 Enhanced procedures for preparing food hydrolysates  
A comestible hydrolysate product is prepared by hydrolyzing a proteinaceous substrate devoid of viable mesophilic microorganisms and spores in a sterile system with a sterile enzyme preparation...
5618690 Method of using an ER-located endoprotease  
The invention concerns novel DNA molecules encoding a modified, endoplasmic reticulum-located "dibasic processing endoprotease" and the use of said endoplasmic reticulum-located "dibasic...
5614379 Process for preparing anti-obesity protein  
The present invention is directed to a novel process of preparing an anti-obesity protein using dipeptidyl-aminopeptidase isolated from the cellular slime mold, Dictyostelium discodeum. The...
5612460 Active carbonates of polyalkylene oxides for modification of polypeptides  
Poly(ethylene glycol)-N-succinimide carbonate and its preparation are disclosed. Polyethylene glycol (PEG) is converted into its N-succinimide carbonate derivative. This form of the polymer reacts...
5612029 Tissue plasminogen activator glycosylation variants with improved therapeutic properties  
The invention concerns tissue plasminogen activator (t-PA) variants which are glycosylated at any of positions 103-105, and are devoid of functional carbohydrate structure at position 117 of...
5607841 Preparation and proteolytic degradation of a macromolecular protein complex from fibrinogen  
A method for preparing a macromolecular protein complex (MPC) from fibrinogen in human plasma by the steps of ammonium sulfate precipitation, dialysis and gel filtration is disclosed. The isolated...
5607840 Protein hydrolysate derived from mucosa tissue  
A protein hydrolyzate is produced from mucosa tissue of pigs, cattle or sheep by providing an aqueous mixture of the tissue, hydrolyzing the tissue with a proteolytic enzyme at a salt...
5602001 Cell-free method for synthesizing a protein  
This invention concerns recombinant RNA molecules comprising a recognition sequence for the binding of an RNA-directed RNA polymerase, a sequence for the initiation of product strand synthesis-and...
5602002 Process for the production of low-chromium protein hydrolyzates  
Described is the chemical hydrolysis of partial hydrolysates obtained by the enzymatic decomposition, in aqueous conditions in the presence of alkaline-earth oxides or hydroxides, of...
5595887 Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment  
Methods are presented for producing and purifying a variable fusion polypeptide which can be purified by affinity chromatography with the binding protein partner. The variable fusion polypeptide...
5593968 Virus-inactivated factor Xa preparation  
A virus-inactivated Factor-Xa preparation with at least 100 units coagulation factor activity per mg protein is described, wherein this preparation is produced by activation of a corresponding...
5593856 Method for producing protein in a cell-free system  
A method for producing protein in a cell-free system uses cell-free extract required for protein production, extracted from a biological cell. According to the present invention, a wide range of...
5591614 Process for the enzymatic preparation of macrolactone  
This invention concerns a process for enzymatically preparing a compound of formula (1) ##STR1## from the compound of formula (2) ##STR2## by way of a microorganism or an acellular preparation...
5589356 Litigation of sidechain unprotected peptides via a masked glycoaldehyde ester and O,N-acyl rearrangement  
A method of chemical ligation of peptides that requires no side chain protecting groups and no activation of the C-α carboxyl group is presented. The method consists of three steps. In the first...
5589373 Thermostable alkaline metalloprotease produced by a hyphomonas and preparation thereof  
Novel thermostable alkaline metalloprotease produced by Hyphomonas which is stabile under conditions of elevated pH. Also included are a method for its production, and compositions containing the...
5589357 Milk protein partial hydrolysate and process for preparation thereof  
Disclosed is a milk protein partial hydrolysate prepared by enzymatic hydrolysis and infant formula prepared therefrom. The hydrolysate has reduced antigenicity and is prepared from a mixture of...
5589364 Recombinant production of biologically active peptides and proteins  
The present invention relates to the recombinant production of amphiphilic peptides with biologically and therapeutically significant activities. In one embodiment, this invention relates to...
5580751 Process for the preparation of C-terminally amidated peptides  
A process for preparing C-terminally amidated peptides, Peptide-NH2, is presented. In a first step, a substrate component is reacted with a nucleophile component in the presence of trypsin or a...
5576026 Lumbricus product and method of making same  
A method of making a lumbricus powder wherein there is provided a quantity of worms which are cleaned and then masticated. The masticated worms are then hydrolyzed, this being accomplished by...
5573923 Method for removing N-terminal dipeptides from precursor polypeptides with immobilized dipeptidylaminopeptidase from dictyostelium discoideum  
A method for removing dipeptides from the amino terminus of precursor polypeptides to produce a polypeptide product is presented which comprises contacting the precursor polypeptide for sufficient...
5571690 Method for the cell-free synthesis of proteins  
A method is disclosed for the reliable production of proteins by amplifying, transcribing and translating the gene encoding the protein in an essentially cell-free system (gene amplification with...
5571689 Method of N-acylating peptide and proteins with diheteroatom substituted analogs of myristic acid  
A method for acylating peptides and proteins with the CoA-ester of diheteroatom substituted C13 to C14 myristic acid analogs catalyzed by N-myristoyltransferase is presented. In the analogs, two...
5569599 Kerainase from fervidobacterium pennavorans DSM 7003  
An enzyme composition containing keratinase is obtained from Fervidobacterium pennavorans DSM 7003. The composition is capable of degradating keratin-containing substrates such as feathers, hair...
5565318 Room temperature stable reagent semi-spheres  
A reagent semi-sphere is disclosed comprising at least one biological reagent and a glass forming filler material in a concentration sufficient to facilitate formation of a glassy, porous...
5563044 Process for the enzymatic preparation of GRF(1-44)NH2  
GRF(1-44)-NH2 is prepared by the trypsin catalyzed enzymatic coupling of Leu-NH2 to GRF(1-43)-OH. The latter compound may be obtained by recombinant DNA synthesis. Thus the present method provides...
5563047 Method for crosslinking of haemoglobin  
Haemoglobin is crosslinked by means of transglutaminase, whereafter the transglutaminase is inactivated. The crosslinked haemoglobin can be used as a molecular agent for oxygen transportation, as...
5561221 Methods and compositions for promoting protein folding  
Disclosed are 1) compositions comprising isolated monomeric subunits of chaperonin-60 or truncated fragments thereof that promote the folding of a polypeptide chain in vitro, 2) monomeric subunits...
5556766 Method of producing LYS-plasminogen  
A process for preparing lys-plasminogen from blood plasma or blood plasma products having a specific activity of at least 17.5 caseinolytic units/mg protein and at lease 50 μmoles/g protein...
5556768 Method for preparing peptides and proteins utilizing ribosome  
A novel method for synthesizing peptides and proteins having a desired amino acid sequence by utilizing an aminoacyl-tRNA and misaminoacyl-tRNA as well as an artificial mRNA in a system for in...
5554508 Process for the enzymatic synthesis of alkyl esters of peptides and peptides, and microparticles therefrom  
A process for the simultaneous enzymatic synthesis of a mixture of alkyl esters of peptides and peptides is presented. In the process, a protein substrate and at least one aliphatic alkanol having...
5543302 Proteases of altered stability to autolytic degradation  
Proteases are susceptible to autolytic degradation. The method of the invention provides a method to identify susceptible autolysis sites which are cleaved to produce a few discrete fragments....
5532124 Genetically engineered bacteria to identify and produce medically important agents  
Microorganisms modified such that their growth in selective media is dependent upon the inhibition of a medically important target function are provided and utilized in methods for the screening...
5527689 Enzymatic coupling of L-phenylalanine methyl ester and N-benzyloxycarbonyl-aspartic acid  
The invention relates to a process for the preparation of N-benzyloxycarbonyl-α-L-aspartyl-L-phenylalanine methyl ester by enzymatic coupling of N-benzyloxycarbonyl-L-aspartic acid and...
RE35277 Cell membrane proteins, compositions containing them and procedure for their preparation  
Compositions for the prevention of, and for the treatment of autoimmune diseases which comprise as active ingredient membrane material shed from autoimmune T lymphocytes, or T lymphocytes...
5525336 Cosmetic containing comeocyte proteins and transglutaminase, and method of application  
A cosmetic composition comprising corneocyte proteins and transglutaminase in a cosmetically acceptable vehicle is provided. Upon application, the ingredients of the cosmetic cross link with the...
5523211 Identification, quantitation and purification of insecticidal proteins from Bacillus thuringiensis  
A process for identifying proteinaceous protoxins expressed by Bacillus thuringiensis genes is disclosed. According to the process, daughter toxins are first generated by subjecting a...
5523237 Protein preparations  
A plastein material is made by reversing the normal hydrolytic activity of a serine protease. The protease produces a plastein material by acting on a proteinaceous substrate. The substrate is...
5520935 Method for production of pea protein hydrolyzate  
The present invention relates to a pea protein hydrolyzate with very high purity and with organoleptic properties. The present invention also relates to a method for producing said pea protein...
5518912 Endopeptidase  
There is provided by the invention a novel composition comprising, in at least partially purified form, an endopeptidase endogenous to biological cells. The endopeptidase is characterized by...
5516650 Production of activated protein C  
Methods are disclosed for producing a protein which has substantially the same biological activity as human protein C or human activated protein C. The protein is produced by mammalian host cells...
5514646 Insulin analogs modified at position 29 of the B chain  
Analogs of human insulin modified at position 29 of the B chain thereof and, optionally, at other positions, have modified physico-chemical and pharmacokinetic properties and are useful in the...
5514565 Enzymatically-produced dimeric IL-2 and a nerve-derived transglutaminase enzyme for its preparation  
The invention provides a new nerve-derived transglutaminase enzyme that converts immune-derived IL-2 to dimeric IL-2 having oligodendrocyte cytotoxic activity. Both the nerve-derived...
5503990 Preparation of health food supplement product  
The present invention relates to a significant improvement in the preparation of a pharmaceutically purified and proven effective product of powdered hormone-free bovine cartilage for the...
5503989 Production of peptide amides  
Methods of preparing a peptide having a C-terminal amide group from peptides having a C-terminal carboxyl group are provided.
5496710 Protease  
A modified protease is disclosed, which is a mutant of the thermostable neutral protease wherein at least one amino acid residue of SEQ ID NO: 1 selected from the group consisting of the 144th...
5492817 Coupled transcription and translation in eukaryotic cell-free extract  
A method for coupling transcription and translation from DNA using a solution comprising a eukaryotic cell-free extract comprising adding a sufficient amount of a magnesium compound to the extract...
5486461 Casein hydrolyzate and method for production of such casein hydrolyzate  
The new casein hydrolyzate does not contain any unhydrolyzed casein and is characterized by a defined molecular weight distribution. The method is characterized by being performed by means of...