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6001604 Refolding of proinsulins without addition of reducing agents  
An improved and efficient process for the production of recombinant human insulin by folding of a proinsulin hybrid polypeptide is provided.
6001559 Method for detecting Listeria monocytogenes via esculin hydrolysis and nucleic acid hybridization  
A method for rapidly detecting non-viral organisms disclosed. The method involves pelleting an enrichment culture of a sample, optionally washing the pellet, resuspending the pellet in an...
5998155 Stable composition of immobilized protein having affinity for biotin  
Compositions and methods for avidin or protein having affinity for biotin immobilized on an inert support material, e.g., agarose, are disclosed. The compositions have high activity levels for...
5998163 Method for in vitro protein synthesis  
A method for performing coupled in vitro transcription and translation reactions is disclosed. In the transcription reaction, the quantity of mRNA produced is limited to a level of less than about...
5989856 Pharmaceutical composition having an endoproteolytic activity; a process for endoproteolytically processing (precursor) proteins and for the (micro)biological production of proteins  
The invention is based on the finding that furin belongs to a family of endoproteolytically active enzymes and relates to a process for the in vitro cleavage of a protein by treating the protein...
5985590 Expression of Carcinoscorpius rotundicauda Factor C in eukaryotes  
CrFC21 cDNA was cloned into two mammalian vectors: pCIneo and pCDNAI, both of which carry the strong CMV promoter for expression in mammalian cell lines. Various CrFC cDNA constructs transformed...
5972596 Nucleic acid constructs containing HIV genes with mutated inhibitory/instability regions and methods of using same  
A method of locating an inhibitory/instability sequence or sequences within the coding region of an mRNA and modifying the gene encoding that mRNA to remove these inhibitory/instability sequences...
5968767 Methods for in vitro protein synthesis  
A method is provided for in vitro protein synthesis in a bacterial extract wherein the reaction mixture comprises a reducing agent and dissolved oxygen (DO2) wherein the DO2 concentration is...
5962404 Enzymatically-produced oligodendrocyte cytotoxic dimeric IL-2 factor  
An enzymatically-producible dimeric IL-2 such as mammalian, more particularly, human dimeric IL-2, having oligodendrocyte cytotoxic activity, is useful as active ingredient of pharmaceutical...
5962254 Nitrogenous composition resulting from the hydrolysis of wheat gluten and process for its manufacture  
The invention relates to a nitrogenous composition resulting from the hydrolysis of wheat gluten by steepwater obtained from the maize starch industry, characterized in that it exhibits a ratio of...
5962244 High-throughput in vitro assays for modulators of peptidyl transferase  
High-throughput in vitro assays are provided for identifying modulators of peptidyl transferase. New solid-phase assays, related compositions, apparatus and integrated systems are provided.
5962253 Oxidative decarboxylation of peptides catalyzed by flavoprotein EpiD  
A method is described for oxidatively decarboxylating a peptide, comprising combining a peptide with EpiD, wherein the peptide comprises at its carboxy terminus the amino acid sequence X1 X2 X3 X4...
5955585 Catalytic condensation method for the preparation of chlorophyll and bacteriochlorophyll derivatives  
Conjugates of chlorophyll (Chl) and bacteriochlorophyll (Bchl) derivatives with amino acids, peptides and proteins are provided by the invention. The amino acid, peptide or protein residue is...
5955363 Vector for in vitro mutagenesis and use thereof  
Site-directed in vitro mutagenesis is obtained utilizing a specially-engineered plasmid vector. The vector is engineered to contain an inactivated genetic marker which marker is capable of being...
5945316 Lactic-acid-containing cyclic depsipeptides having 18 ring atoms as endoparasiticidal agents, and process for their preparation  
The present invention relates to a process for the preparation of lactic-acid-containing, optically active, cyclic depsipeptides having 18 ring atoms with the aid of fungal strains of the species...
5945299 Production of wheat protein hydrolyzates by multistage hydrolysis with a proteinase and peptidase  
Wheat protein hydrolyzates are produced by hydrolyzing a wheat protein-containing starting material, preferably a wheat protein isolate having a protein content of 70 to 90%, with a proteinase...
5942488 Haemoglobin  
Disclosed is a mutant human β globin modified so as to exhibit, when present as part of a hemoglobin molecule, an altered bicarbonate effect compared to the unmodified β globin. The β globin is...
5935853 Human epithelial cell matrix and uses therefor  
A method of growing epithelial cells by contacting the cells with the extracellular matrix deposited or secreted by human MCF-10A mammary epithelial cells. The MCF-10A matrix stimulates cell...
5935823 Totally synthetic affinity reagents  
A novel process for producing novel and/or improved heterofunctional binding fusion proteins termed Totally Synthetic Affinity Reagents (TSARS) is disclosed. TSARs are concatenated...
5910426 Protein tyrosine kinase  
The present invention is directed to a novel protein tyrosine kinase comprising a polypeptide having multiple protein kinase catalytic domains and, more particularly, two kinase catalytic domains...
5891663 Process for preparing polypeptide  
A method for converting a precursor of a polypeptide that induces IFN-γ production in immunocompetent cells, characterized in that it comprises a step of contacting an interleukin-1β converting...
5879950 Materials and methods for digestion of DNA or RNA using restriction endonucleases  
Disclosed are improvements for enzyme-catalyzed reactions involving DNA or RNA, including restriction digests, which are based on conducting such reactions in the presence of lipids.
5877000 Keratinase produced by Bacillus licheniformis  
Bacillus licheniformis O.W.U. 138B and 88B and other bacterial strains capable of degrading feathers isolated from wild birds, are disclosed. The strains and the keratinases produced by these...
5869278 Process for the preparation of mature human growth hormone by enzymatic hydrolysis of a soluble precursor with immobilized factor Xa  
Mature Human Growth Hormone is prepared by enzymatic hydrolysis of a soluble precursor with immobilized Factor Xa. The soluble precursor contains a cleavage recognition sequence,...
5869444 Osmotic agents for peritoneal dialysis  
This invention relates to a peritoneal dialysis solution which comprises as an osmotically active agent an osmotically effective amount of a mixture of peptides, the mixture consisting...
5869279 Modified porphyroproteins  
Hemin-like porphyrins can be prepared from chlorophylls and hemin, which may be used as intermediates to form functionally active porphyroproteins. The porphyrins can be reacted with apoproteins...
5866357 Method for hydrolyzing proteins with gluyasp specific protease  
A method is disclosed for obtaining limited specific hydrolysis of proteins using a Glu/Asp specific protease to produce peptides with C-terminal Glu or Asp. The protease is obtained from Bacillus...
5856166 Streptomyces proteases and methods for improved secretion of recombinantly-expressed proteins  
A family of proteases endogenous to Streptomyces cells degrades heterologous proteins secreted from Streptomyces host cells. The previously unidentified proteases include (1) tripeptidyl...
5856082 Devices and methods for characterizing proteins and peptides  
The present invention provides analytical devices for the characterization of the primary structure of proteins and peptides, comprising a microenzyme reactor, a separation device, an interface...
5851791 Ubiquitin conjugating enzyme (E2) fusion proteins  
A novel class of fusion proteins based on the ubiquitin-conjugating enzyme, or E2, is described. The fusion proteins include, in addition to the E2 activity, a protein binding ligand having a...
5851792 Aglucone isoflavone enriched vegetable protein whey whey protein material aglucone isoflavone material high genistein content material and high daidzein content material and process for producing the same from a vegetable protein whey  
An aglucone isoflavone enriched vegetable protein whey, whey protein material, high genistein material, high daidzein material, and aglucone isoflavone material are provided, as well as a process...
5851786 Product and process to regulate actin polymerization  
The present invention relates to methods useful for identifying compounds capable of specifically regulating actin polymerization, stress fiber formation or focal adhesion assembly by regulating...
5849686 Morphogen-induced liver regeneration  
Disclosed are therapeutic treatment methods, compositions and devices for maintaining liver function in a mammal, including methods, compositions and devices for regenerating lost or damaged...
5846929 Purification of type G botulinum neurotoxin and pharmaceutical compositions thereof  
The isolation and purification of type G botulinum neurotoxin and complexes thereof is disclosed. Compositions containing the type G neurotoxin and the preparation of a type G toxoid are also...
5843735 Aklavinone C-11 hydroxylase, gene coding for same, expression vector therefor, and process for preparing hybrid antibiotics by using said vector  
Aklavinone C-11 hydroxylase separated from Streptomyces sp.; a gene encoding same; an expression vector comprising said gene; a microorganism transformed with said vector; and a process for...
5840315 Vaccines based on streptokinase  
The present invention provides a vaccine to treat or prevent mastitis in a bovine species, comprising an immunologically effective amount of a plasminogen-activating streptokinase protein produced...
5840295 Nerve-derived transglutaminase enzyme  
The invention provides a new nerve-derived transglutaminase enzyme that converts immune-derived IL-2 to dimeric IL-2 having oligodendrocyte cytotoxic activity. Both the nerve-derived...
5840517 Process for preparing obesity protein analogs  
The present invention is directed to an improved process for preparing in high yield an obesity protein analog using a dipeptidylaminopeptidase isolated from the slime mold, Dictyostelium discoideum.
5837483 Enzymatic method for producing N-formyl-α-L-aspartyl-L-phenylalanine methyl ester  
An enzymatic method for producing N-formyl-α-L-aspartyl-L-phenylalanine methyl ester by a condensation reaction between N-formyl-L-aspartic acid and L-phenylalanine methyl ester or...
5834251 Methods of modifying carbohydrate moieties  
The invention is directed to methods of converting high mannose type glycosylation patterns to hybrid or complex type glycosylation patterns.
5834232 Cross-linked gelatin gels and methods of making them  
Enzymatically cross-linked protein gels and methods for preparing them are disclosed. The methods comprise adding a transglutaminase, such as factor XIII, to a composition of a...
5830740 Serine protease operative between 75°C. and 103°C.  
A subtilisin which exhibits proteolytic activity at temperatures ranging from 75° C. to 130° C. The subtilisin has been given the name aerolysin because it was initially isolated from the...
5830666 Method of detecting nucleic acids or the like  
A method of detecting nucleic acids, proteins, or protein nucleic acid complexes. The method includes binding an enzyme, such as phosphatase, to a specimen of the nucleic acid, protein, or protein...
5831025 Human activated protein C and process for preparing same  
A human Activated Protein C preparation with a high specific activity of 3500 U/mg or more and substantially free from thrombin or other proteases which can convert Protein C into Activated...
5827073 Photoreactive peptide derivatives  
The invention relates to methods for making synthetic, photoreactive peptides and their use. A photoreactively labelled amino acid is incorporated into the peptide. The resulting peptide, when...
5827682 Two-step conversion of vegetable protein isoflavone conjugates to aglucones  
Disclosed are methods for converting isoflavone conjugates in vegetable protein materials to isoflavone aglucones by forming an aqueous mixture of a vegetable protein material containing...
5824780 Activated human factor VIII and method of preparation  
A method for preparing a purified and stable activated human Factor VIII composition is disclosed. A purified and stable activated human Factor VIII composition having a specific activity of at...
5821047 Monovalent phage display  
A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method...
5814460 Method for generating and screening useful peptides  
The invention allows the generation and screening of a large population of peptides for the presence of peptides which bind a particular macromolecule or macromolecular complex with high affinity,...
5814328 Preparation of collagen using papain and a reducing agent  
Purified collagen is produced by contacting a collagen sample with a first proteolytic enzyme, a reducing agent and a second proteolytic enzyme. Preferably, the first and second proteolytic...