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8168434 |
Process for chromosomal integration and DNA sequence replacement in Clostridia
The present invention is related to a new method for replacing or deleting DNA sequences in Clostridia, with high efficiency, easy to perform and applicable at an industrial level. This method is...
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8163557 |
Chimaeric human papillomavirus 16 L1 virus-like particles and a method for preparing the particles
A method for producing a chimaeric human papillomavirus (HPV) L1 polypeptide containing a heterologous peptide, and in particular, a HPV L2 peptide comprising the steps of introducing a DNA...
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8148155 |
Methods for increasing homologous recombination of a nucleic acid sequence
The present invention relates to methods for increasing homologous recombination of a nucleic acid sequence introduced into a host cell, comprising: (a) introducing into a population of filamentous...
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8148130 |
T4 bacteriophage bound to a substrate
T4 bacteriophages are bound to substrates such as liposomes using a binder.
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8088621 |
Adenoviral fiber exchange shuttle system
The instant invention provides methods and compositions for generating recombinant adenoviral vectors. The invention also provides kits comprising for the generation of recombinant adenoviral vectors.
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8067157 |
Expression cloning in filamentous fungi
Methods are provided for isolation of DNA sequences encoding proteins with properties of interest by means of expression cloning in filamentous fungal host cells. The isolated DNA sequences are...
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7972857 |
Methods for the replacement, translocation and stacking of DNA in eukaryotic genomes
The present invention includes compositions and methods for site-specific polynucleotide replacement in eukaryotic cells. These methods include single polynucleotide replacement as well as gene...
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7883894 |
Process for the reversible functional inactivation of the mismatch repair system
The present invention relates to a process for allowing homologous recombination between non-identical DNA sequences of an organism and various applications thereof.
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7807444 |
Circular DNA molecule having a conditional origin of replication, process for their preparation and their use in gene therapy
A circular DNA molecule, useful for gene therapy, comprising at least one nucleic acid sequence of interest, characterised in that the region allowing the replication thereof has an origin of...
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7803602 |
Method for overexpression of zwitterionic polysaccharides
The present invention is directed to methods for producing and selecting new mutant strains of B. fragilis that constitutively express a particular capsular polysaccharide or only selected capsular...
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7799904 |
Gilvocarcin gene cluster, recombinant production and use thereof
A nucleic acid molecule encoding the gilvocarcin V gene cluster and subunits thereof. Recombinant vectors and host cells comprising a nucleic acid compound encoding the gilvocarcin V gene cluster...
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7785840 |
Method of production of L-amino acids
An isolated polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, with the L-aspartic acid at position 5 of the amino acid sequence replaced by another...
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7736886 |
Recombination systems and methods for eliminating nucleic acid sequences from the genome of eukaryotic organisms
The invention relates to recombination systems and methods for eliminating nucleic acid sequences from the chromosomal DNA of eukaryotic organisms, and to transgenic organisms—preferably p...
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7700328 |
Method for producing an L-tyrosine over-producing bacterial strain
An enteric bacterial strain was engineered to over-produce L-tyrosine using a one-step method. The pheA-tyrA chromosomal region of the bacterial genome was replaced with an engineered chromosomal...
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7678558 |
Method for overexpression of zwitterionic polysaccharides
The present invention is directed to methods for producing and selecting novel mutant strains of B. fragilis that constitutively express a particular capsular polysaccharide or only selected...
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7514216 |
Methods for generating hypermutable yeast
Yeast cells are mutagenized to obtain desirable mutants. Mutagenesis is mediated by a defective mismatch repair system which can be enhanced using conventional exogenously applied mutagens. Yeast...
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7479389 |
ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and methods for stable genetic integration
Novel genes encoding P. pastoris ARG1, ARG2, ARG3, HIS1, HIS2, HIS5 and HIS6 are disclosed. A method for inactivating alternately at least two biosynthetic pathways in a methylotrophic yeast is...
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7468190 |
Reduction in the virulence of Mycobacterium tuberculosis and protection against tuberculosis by means of phoP gene inactivation
The construction of a mutant in the phoP gene by means of homologous recombination from a clinically isolated Mycobacterium tuberculosis reduces the virulence thereof in mouse bone marrow...
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7413732 |
Baculovirus expression system
The application relates for method for cloning the gene comprising the steps of: 1. Providing a replication-deficient baculovirus vector, 2. Providing a rescue vector comprising (a) nucleic acid...
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7364745 |
Development of a live, attenuated, recombinant vaccine for Brucellosis
A recombinant, attenuated strain of Brucella suis or Brucella melitensis with a deficiency in carboxyl-terminal protease activity or tail-specific protease activity can be used as a vaccine for the...
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7332341 |
Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
The present invention relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells.
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7317216 |
Ultrasensitive biochemical sensing platform
An electronic sensor is provided for detecting the presence of one or more targets of interest in a sample. The sensor preferably comprises a special type of field effect transistor in which...
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7309605 |
Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful...
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7271000 |
Nucleotide sequence encoding the enzyme I-Scel and the uses thereof
An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful...
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7175983 |
Adapter-directed display systems
The present invention provides adapter-directed display systems for expressing exogenous polypeptide within a host cell and/or displaying the exogenous polypeptide on the outer surface of a genetic...
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7129079 |
Cells engineered to contain genes of interest
Methods and materials are provided for stably introducing any gene into a specific locus in the genome of a microorganism such as yeast without the addition of any drug resistance genes....
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7070930 |
Chimeric vectors comprising a phage packaging site and a portion derived from the genome of a eukaryotic virus
The present invention provides an improved method of making eukaryotic gene transfer vectors comprising homologous recombining lambdid vectors with a second DNA in a bacterium to generate novel...
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7056728 |
Compositions and methods for use thereof in modifying the genomes of microorganisms
The present invention relates to bacterial luciferase transposon cassettes suitable for conferring bioluminescence properties on a Gram-positive bacteria, Gram-negative bacteria, and other...
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7049145 |
Vaccinia virus MVA-E3L-knockout-mutants and use thereof
The present invention relates to mutant MVA vaccinia viruses, which are used for the generation of recombinant MVA viruses, as well as host cells, which have been infected with these mutant MVA...
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7033814 |
Methods for preparing yeast with improved biotin productivity using integrating plasmids encoding biotin synthase
Methods for preparing yeast with improved biotin productivity using integrating plasmids encoding biotin synthase. The yeast is transformed by an integrating plasmid, which includes a Candida...
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6830920 |
Rapid generation of recombinant adenoviral vectors
The invention provides a novel Adenovirus backbone plasmid, which when co-transfected with a shuttle vector, allows for production of recombinant viruses quickly and easily. The present invention...
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6830885 |
Nucleic acid molecule, method and kit for selecting a nucleic acid having a desired feature
This invention relates to the screening of nucleic acids. More particularly, the present invention provides a dysfunctional viral genome capable of both expressing libraries of exogenous nucleic...
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6828093 |
Rapid subcloning using site-specific recombination
The present invention provides compositions, including vectors, and methods for the rapid subcloning of nucleic acid sequences in vivo and in vitro. In particular, the invention provides vectors...
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6808896 |
Method for stable chromosomal multi-copy integration of genes
The present invention solves the problem of integrating multiple copies of a gene of interest by homologous recombination into well defined positions adjacent to conditionally essential genes in a...
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6762040 |
Method for increasing gene copy number in a host cell and resulting host cell
The invention relates to a method for increasing the copy number of a chromosomally integrated expression cassette in a microbial strain without leaving antibiotic resistance markers behind in the...
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6750333 |
Process for preparing purified nucleic acid and the use thereof
The invention relates to a nucleic acid preparation with a content of below 1% protein, preferably below 0.1% protein, free of ethidium bromide, phenol, caesium chloride and detergents based on...
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6746870 |
DNA recombination in eukaryotic cells by the bacteriophage PHIC31 recombination system
This invention provides methods for obtaining specific and stable integration of nucleic acids into eukaryotic cells. The invention makes use of site-specific recombination systems that use...
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6723562 |
DNA construct for transforming a yeast
A DNA construct comprising: (1) a selective marker gene,(2) a galactose-inducible growth inhibition sequence,(3) a pair of FRT sequences in the same orientation flanking (1) and (2), and(4) a DNA...
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6645766 |
Shuttle vectors
The invention provides shuttle vectors, and methods of using shuttle vectors, capable of expression in, at least, a mammalian cell. Furthermore, the shuttle vectors are capable of replication in at...
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6617163 |
Polynucleotides for mutagenesis in fungus comprising a functional gene in magnaporthe and an impala transposon
The invention concerns a new tool for efficient mutagenesis enabling the generation of a collection of mutants in fungi by random insertion of a characterized Fusarium oxysporum Impala transposon...
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6602712 |
Enterotoxin-deficient bacillus
A enterotoxin-deficient mutant of a member strain of the Bacillus cereus group does not produce HBL enterotoxin, which has been regarded as a human pathogen found in member strains. An...
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6599697 |
Process for preparing a multicombinatorial library of vectors for expressing antibody genes
On the basis of a first repertoire of genes coding for a population of one of two kinds of polypeptides capable of being combined, particularly an antibody light chain variable region, and at least...
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6596516 |
Process for the fermentative preparation of L-amino acids using coryneform bacteria
A process for the preparation of L-amino acids, in which the following steps are carried out, a) fermenting the desired L-amino acid-producing bacteria in which at least the glyA gene is...
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6586214 |
Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene)
The present invention is directed to isolated polynucleotides coding for phosphoglucose isomerase (pgi) from coryneform bacteria. In addition, the invention includes methods for increasing the...
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6582693 |
Recombinant MVA virus expressing the E2 gene of Bovine papillomavirus and its use in the therapy of tumors generated by papillomaviruses
A recombinant vaccinia virus derived from the vaccinia virus Ankara (MVA) encoding and capable of expressing the E2 gene of Bovine papillomavirus. Also, the use of the virus in the treatment of...
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6548246 |
Method and probes for the identification of microbial genes specifically induced during host infection
The present invention relates to a class of microbial coding sequences the transcription or cotranscription of which is specifically induced during microbial infection of a host. These particular...
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6534315 |
Yeast transformation cassette
The present invention relates to a DNA cassette intended for the transformation of yeast, leaving no useless exogenous DNA but the gene(s) of interest comprising at least one negative dominant...
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RE38028 |
Molecular genetic construction of vaccine strains of pasteurellaceae
Tools for genetically engineering Pasteurellaceae are provided. Replication-conditional plasmids which are useful for the Pasteurellaceae have been isolated and characterized. The plasmids can be...
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6451582 |
Staphylococcus strains comprising an inducible gene encoding an RNA polymerase specificity factor
The present invention relates to a recombinant organism, and a bacterial strain such as Staphylococcus, and S. aureus, in particular. The organism has a regulatable gene for encoding an RNA...
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6448034 |
Production of variant nisin
The invention provides a method for making a cell which does not contain a natural nisA gene but expresses a variant nisA gene, said method comprising the steps of providing a cell that contains a...
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