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7550296 |
Generation of replication competent viruses for therapeutic use
The present invention relates to the generation of replication-competent viruses having therapeutic utility. The replication-competent viruses of the invention can express proteins useful in the...
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7456020 |
Method for modifying chromosomes
The present invention provides a simple method for splitting and loss of a chromosome in yeast. The method for modifying a chromosome in yeast includes preparing a linear chromosome splitting...
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7393639 |
Methods for identifying genes and promoters of interest using the Impala transposon
The invention concerns a new tool for efficient mutagenesis enabling the generation of a collection of mutants in fungi by random insertion of a characterised Fusarium oxysporum Impala transposon...
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7338801 |
Double transposition methods for manipulating nucleic acids
Methods are provided for manipulating nucleic acid to produce gene fusions, to delete or clone a portion of a chromosome, or to insert a sequence into a chromosome. The methods employ sequential...
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7262056 |
Enhancing intermolecular integration of nucleic acids using integrator complexes
We disclose compositions and processes for enhancing transposon mediated integration of a nucleic acid molecule into another target nucleic acid molecule. Integration by an integrator complex is...
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7169393 |
Antigenic peptide fragments of VapA protein, and uses thereof
An isolated peptide fragment of the VapA protein that binds antibodies specific for Rhodococcus equi and the VapA protein. In a preferred form the peptide contains an amino acid sequence of 5 or...
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7138279 |
Transposon-based transformation system
A transposon-based mutagenesis method for altering DNA in Sorangium and other Myxococcales host cells is provided, along with vectors and transposases for use in the method.
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7125662 |
Method of identifying putative antibiotic resistance genes
The present invention relates to methods of identifying putative antibiotic resistance genes. According to one embodiment, this is carried out by first isolating a microbial DNA molecule or cDNA...
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7101713 |
DNA transformation efficiency by inhibiting host cell restriction
It can be difficult to achieve efficient transformation of many strains of bacterial cells due in part to the presence of one or more restriction and modification (R-M) systems in the cells that...
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7090994 |
Methods of screening for introduction of DNA into a target cell
The present invention provides methods of introducing a polynucleotide into a target cell, wherein the method employs a light generating protein coding sequence acting as a reporter. An important...
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7056728 |
Compositions and methods for use thereof in modifying the genomes of microorganisms
The present invention relates to bacterial luciferase transposon cassettes suitable for conferring bioluminescence properties on a Gram-positive bacteria, Gram-negative bacteria, and other...
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7001893 |
Inclusion complex of Rifampicin, an anti-tubercular drug, with β-cyclodextrin or 2-hydroxypropyl β-cyclodextrin and a process thereof
The present invention relates to an inclusion complex of Rifampicin and cyclodextrin (CD) that can be used as an anti-tubercular drug. The present invention also relates to a process for...
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6991900 |
Methods for identifying an essential gene in a prokaryotic microorganism
Methods are provided for the rapid identification of essential or conditionally essential DNA segments in any species of haploid cell (one copy chromosome per cell) that is capable of being...
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6962810 |
Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector piggyBac
More efficient transfer of genes into host cells or embryos to transform the cells or embryos is facilitated by transposition vectors using the minimal amount of nucleotide sequences in the...
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6943012 |
Helper dependent adenoviral vector system and methods for using the same
A helper dependent adenoviral vector system is provided. The subject helper dependent adenoviral vector system is made up of: (1) a “gutless” adenoviral vector that include cis-acting human...
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6849429 |
Viral pseudo-capsids including assembly agonists and antagonists
Recombinant hepatitis C virus (HCV) capsid proteins that self-assemble into large spherical virus-like particles structures and viral capsids that include conformational antigenic epitopes are...
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6841375 |
Flavobacterium heparinum expression system
This invention is directed to Flavobacterium heparinum for use as a host cell organism for the expression of homologous and heterologous genes.
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6787360 |
Bacteriophage, a process for the isolation thereof, and a universal growth medium useful in the process thereof
The present invention provides a isolated bacteriophage useful as a tool for studying biological, biochemical, physiological and genetic properties of actinomycetes and other organisms which...
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6664082 |
Genetically engineered L-sorbose reductase-deficient mutants
The present invention provides a genetically engineered microorganism belonging to the genus Gluconobacter or Acetobacter, which has an engineered gene for the biological activity of reducing...
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6617163 |
Polynucleotides for mutagenesis in fungus comprising a functional gene in magnaporthe and an impala transposon
The invention concerns a new tool for efficient mutagenesis enabling the generation of a collection of mutants in fungi by random insertion of a characterized Fusarium oxysporum Impala transposon...
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6599705 |
Regulation of fungal gene expression
Disclosed herein are methods for regulating fungal gene expression, and reagents for carrying out those methods.
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6582951 |
Method for non-homologous transformation of Yarrowia lipolytica
The invention concerns the integration of a gene of interest into the genome of a Yarrowia strain devoid of zeta sequences, by transforming said strain using a vector bearing zeta sequences.
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6528257 |
Method for the simultaneous monitoring of individual mutants in mixed populations
The present invention relates to an improved and efficient method for simultaneous monitoring of abundance of individual mutants of a microbe in mixed populations where insertion of a known...
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6518481 |
Universal markers of transgenesis
The invention relates to methods, cells and nucleic acids for making transgenic animals. The methods generally comprise introducing into a genome of an animal a genetic construct comprising a...
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6504081 |
Methods and uses for transposon-based gene targeting
The present invention provides methods which greatly facilitate the rapidity in which cells and transgenic animals with targeted genes may be generated. The invention hastens the investigation of...
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6485912 |
Methods of performing gene trapping in bacterial and bacteriophage-derived artificial chromosomes and use thereof
A method of efficiently sequencing multiple exons from complex genomic DNAs is disclosed. The methodology includes the use of bacterial and bacteriophage-derived artificial chromosomes (BBPACS) in...
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6448034 |
Production of variant nisin
The invention provides a method for making a cell which does not contain a natural nisA gene but expresses a variant nisA gene, said method comprising the steps of providing a cell that contains a...
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6303381 |
Insertion sequence
A novel insertion sequence, which has been found in the sMMO gene coding for methane monooxygenase of methane-assimilating bacterium Methylococcus capsulatus NCIMB 11132 strain, and has inverted...
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6221364 |
Recombinant mycobacteria auxotrophic for diaminopimelate
The present invention refers in general to novel recombinant mycobacteria that are auxotrophic for diaminopimelate. In particular, this invention relates to novel auxotrophic recombinant...
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6207384 |
Systematic identification of essential genes by in vitro transposon mutagenesis
The invention features a general system for the identification of essential genes in organisms. This system is applicable to the discovery of novel target genes for antimicrobial compounds, as well...
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6197590 |
Process for integration of a chosen gene on the chromosome of a bacterium obtained by the said process
The present invention relates to a process for integration of a chosen gene or of a specific DNA sequence in a DNA sequence such as the chromosome or episome of a bacterium, wherein: a) the said...
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6159736 |
Method for making insertional mutations using a Tn5 synaptic complex
A method for making insertional mutations at random or quasi-random locations in the chromosomal or extra-chromosomal nucleic acid of a target cell includes the step of combining, in the target...
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6096549 |
Method of selection of allelic exchange mutants
A process for replacing a nucleotide sequence in the genome of a mycobacterium strain comprises the steps of: a) providing a vector containing SacB gene coding for levane saccharase enzyme and a...
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5994137 |
L5 shuttle phasmids
This invention is directed to L5 shuttle phasmids capable of delivering foreign DNA into mycobacteria and to methods of producing L5 shuttle phasmids. In addition, this invention is directed to a...
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5985570 |
Identification of and cloning a mobile transposon from Aspergillus
There are provided novel transposable elements isolated from Aspergillus. Also provided are novel fragments comprising the inverted repeat(s) of the transposable elements, such fragments being...
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5972700 |
TM4 conditional shuttle phasmids and uses thereof
The present invention provides a conditional shuttle phasmid constructed by inserting a cosmid into a non-essential region of the TM4 mycobacteriophage that introduces DNA of interest into...
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5958775 |
Composition and method for targeted integration into cells
A vector containing a selected nucleic acid sequence interposed between the terminal ends of a Tn7 transposon and a method for site specific integration of genetic material into a chromosome using...
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5900362 |
Luminescent nitrifying microorganisms
The method of producing luminescent bacteria is based on the transfer of genetic information for biological luminescence to obligate chemolithoautotrophic nitrifying microorganisms. In particular,...
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5882888 |
DNA integration by transposition
Multicopy strains of gram-positive bacteria carrying multiple copies of a DNA sequence of interest may be constructed by use of a method involving introduction of a DNA construct comprising the DNA...
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5869296 |
DNA transposon Tn5seq1
A novel transposon useful for sequencing long DNAs is disclosed which comprises a partial sequence of transposon Tn5 with the oligonucleotide primers from phages SP6 and T7 inserted near the...
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5837509 |
Recombinant lactic acid bacterium containing an inserted promoter and method of constructing same
A method of isolating a lactic acid bacterial DNA fragment comprising a promoter, the method comprising introducing a DNA molecule comprising a transposable element comprising a promoterless...
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5804414 |
Method of amplifying genes using artificial transposons in coryneform bacteria
A method of amplifying a desired gene in a chromosome of a coryneform bacterium, which comprises forming an artificial transposon in which a drug resistance gene and the desired gene are inserted...
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5786162 |
Fused genes and their use for determining the presence of metals or of xenobiotic compounds
The invention relates to a fused gene containing: the promoter sequence of (a) gene(s) encoding the resistance to one or several metal(s) or encoding the catabolism of one or several xenobiotic...
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5773267 |
D29 shuttle phasmids and uses thereof
The present invention provides a conditional shuttle phasmid constructed by inserting a cosmid into a non-essential region of the D29 mycobacteriophage which is capable of introducing DNA of...
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5728551 |
In vitro transposition of artificial transposons for DNA sequencing
We have developed efficient methods of creating artificial transposons and inserting these transposons into DNA targets in vitro, primarily for the purpose of mapping and sequencing DNA. A target...
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5645991 |
Transposon-containing DNA cloning vector and uses thereof
The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method...
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5643771 |
Non-reverting live bacterial vaccines
Live vaccines are provided and methods for preparing the live vaccines for protection of a host from a pathogenic microorganism. The vaccines are prepared by introducing at least one modification...
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5633154 |
Method for location of insertion elements
A method of locating insertion elements (IS elements) or transposons in coryneform bacteria, a positive selection system suitable for the above, the IS elements found in this manner and their use,...
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5591577 |
Mobile genetic element originated from brevibacterium strain
A DNA fragment which contains a mobile genetic element originated from a strain of the genus Brevibacterium is obtained, and a gene is transferred into the chromosomal DNA of the strain making use...
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5516669 |
Transposon consisting of a fragment of the R plasmid pCxM82B, test vectors containing this transposon, and methods of mutagenesis
The invention relates to a transposon consisting of a fragment of the R plasmid pCxM82B from Corynebacterium xerosis DSM 5021, to mobilizable, non-self-transferrable vectors which contain this...
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