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7399624 |
Cephalosporin C acylases
Enzymes with cephalosporin C acylase activity obtained by site-directed, random or “site-saturation” mutagenesis of a native sequence optimised for expression in E. coli.
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7264943 |
Method for producing cephalexin
The invention relates to a process for the preparation of cephalexin with the aid of a penicillin amidase immobilized on a crosslinked hydrophilic copolymer which has binding activity for ligands...
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7241588 |
Method for producing novel β-lactam antibiotic from protoplast fusion strain
The present invention provides a method for producing a novel β-lactam antibiotic from a protoplast fusion strain. The method is to fermentatively culture the protoplast fusion strain of ...
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7229784 |
Modulation of secondary metabolite production by zinc binuclear cluster proteins
Methods for improving the production of a secondary metabolite by a fungus by increasing the yield or productivity of the secondary metabolite produced by the fungus are described. The methods...
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7195892 |
Acylase gene
The subject of the present invention is to provide a β-lactam acylase protein having high activity, a gene coding for said β-lactam acylase protein, a recombinant vector having said gene, a...
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6815189 |
Gene from acremonium chrysogenum encoding a protein with cephalosporin C acetylhydrolase activity and methods of use of such gene
An isolated DNA sequence that encodes a peptide with CPC-acetylhydrolase enzymatic activity is disclosed. Also, a method of expressing CPC-acetylhydrolase activity that includes the steps of (a)...
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6730497 |
Process for preparing cephalosporanic acid derivatives using β-ketoacid derivatives
A process for preparing cephalosporanic acid derivatives comprises the steps of enzymatically converting a 3-thiolated cephalosporin C compound of formula III: into a...
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6699699 |
Mutated penicillin expandases
A mutated expandase enzyme having higher activity on penicillin G is provided to produce phenylacetyl-7-aminodeacetoxycephalosporanic acid (7-ADCA), which mutated expandase enzyme has one or more...
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6649369 |
Process for delaying the deactivation of glutaryl amidase during enzyme catalysis
The present invention relates to a process for delaying the deactivation of glutaryl amidase during enzyme catalysis. The enzyme catalyst is, after maximum conversion of the substrate, separated...
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6642020 |
Process for preparing cephalosporin derivatives
An enzymatic process for preparing 3-thiolated 7-aminocephalosporanic acid derivatives comprises the steps of enzymatically converting a 3-thiolated cephalosporin C of the formula I: to form a...
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6518039 |
Process for the fermentative production of deacylated cephalosporins
The present invention discloses a process for the production of N-deacylated cephalosporin compounds via the fermentative production of their 7-acylated counterparts.
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6503727 |
Process for the preparation of an antibiotic
The invention relates to a process for the preparation of an antibiotic, in particular cefalexin, ampicillin, amoxicillin, cefaclor, cefradin, cefadroxil, cefotaxim and the like, wherein a...
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6458558 |
Process for the preparation of β-lactam derivatives
A process for the preparation of Cefuroxime acid (I), which comprises the following steps: (1) Extraction of deacetyl 7-glutaryl ACA (II) aqueous solution at acid pH with organic solvents (for...
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6383773 |
Penicillin conversion
The present invention provides a biological system for expanding the dethiazolidine ring of penicillins into the dehydrothiazine ring of cephalosporins or cephalosporin precursors. In particular,...
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6337190 |
D-amino acid aminotransferase for simultaneously producing glutaryl-7-aminocephalosporanic acid and D-amino acid
The present invention relates to mutant D-amino acid aminotransferase, including nucleic acids encoding mutant D-amino acid aminotransferase. The mutant D-amino acid aminotransferase of the present...
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6319684 |
Process for the fermentative production of cephalosporin
The present invention relates to a novel process for the preparation of cephalosporins having been deacylated at the 7-amino group, by fermentation of a cephalosporin producing microorganism in the...
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6297032 |
Recombinant cephalosporin C amidohydrolase in cephalosporin biosynthesis
The present invention relates to an enzyme process for the one-step conversion of cephalosporin C or a derivative thereof into 7-aminocephalosporanic acid or a corresponding derivative thereof. The...
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6258555 |
DNA encoding ACV synthetase
DNA encoding the gene for the synthetase enzyme capable of generating δ (L-a-aminoadipyl)-L-crysteinyl-D-valine (ACV) from its constituent amino acids was obtained from several penicillin and...
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6218138 |
Synthesis of beta-lactam antibiotics with immobilized penicillin amidase
Beta-lactam antibiotics are synthesized by reacting an amino-beta-lactam component with a corresponding amino-group-containing acylating side-chain component in the presence of penicillin amidase...
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6180361 |
Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase and deacetylcephalosporin C synthetase
The present invention provides DNA compounds that encode the expandase/hydroxylase enzyme of Cephalosporium acremonium. The compounds can be used to construct recombinant DNA expression vectors for...
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6171814 |
Process to increase the production of clavam antibiotics
A process for increasing the amount of clavam produced by an organism having both a clavam pathway or a portion thereof and a cephalosporin pathway or a portion thereof by interfering with the...
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6156534 |
Synthesis of β-lactam antibacterials using soluble side chain esters and enzyme acylase
Disclosed is a process for the synthesis of β-lactam antibacterials using soluble side chain esters in the presence of enzyme acylase. Also disclosed are novel esters useful as reactants in said...
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6146871 |
Process for modifying the enzyme 7β-(4-carboxybutanamide) cephalosporinacylase and purifying said enzyme in a single chromatographic step
A procedure for producing a modified 7β-(4-carboxybutanamide) cephalosporinase enzyme which can be purified in a single chromatographic step. The procedure for production of the enzyme involves:...
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6071713 |
Bioprocesses for preparing 7-ACA and 7-ADAC
A bioprocess for preparing adipoyl-7-ACA comprising the steps: (a) transforming cells of a strain of Penicillium chrysogenum which produces isopenicillin N with an expression vector containing DNA...
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6071712 |
Enzymatic process for the production of cephalosporins
An enzymatic process for hydrolyzing the 7-position acyl ester of certain cephalosporin compounds to the corresponding alcohol is disclosed.
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6020151 |
Process for the production of 7-ADCA via expandase activity on penicillin G
A process is taught for the preparation and recovery of 7-aminodesacetoxycephalosporanic acid (7-ADCA) via enzymatic ring expansion activity on penicillin G, using a Penicillium chrysogenum...
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5942411 |
Process of producing B-lactam antibiotics applying microorganisms with increased ligase activity
The present invention relates to the biosynthesis of β-lactam antibiotics. More specifically, the invention relates to processes of producing β-lactam antibiotics in vivo and in vitro. Also...
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5935831 |
Mutated β-lactam acylase genes
New mutant β-lactam acylases are provided exhibiting altered substrate specificities. These β-lactam acylases are obtained by expression of a gene encoding said β-lactam acylase and having an...
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5922907 |
Precursors of β-lactam antibacterials having soluble side chain esters
Disclosed is a process for the synthesis of β-lactam antibacterials using soluble side chain esters in the presence of enzyme acylase. Also disclosed are novel esters useful as reactants in said...
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5919648 |
Industrial enzymes
The present invention relates to an enzyme process for the one-step conversion of cephalosporin C or a derivative thereof into 7-aminocephalosporanic acid or a corresponding derivative thereof. The...
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5874571 |
Process for the recovery of cephalexin
The disclosed process is for the recovery of cephalexin from a mixture containing cephalexin and 7-aminodesacetoxy cephalosporanic acid (7-ADCA), wherein a mixture of cephalexin and 7-ADCA, with a...
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5801011 |
Process for preparing cephalosporins with penicillin acylase without pH control
The invention relates to an improved process for preparing cephalosporins by reaction compounds such as 7-amino-cephalosporanic acid, 7-amino-3-deacetoxy-cephalosporanic acid or their derivatives...
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5795733 |
Process for the efficient production of 7-ADCA via 3-(carboxyethylthio) propionyl-7-ADCA
An overall efficient process for the preparation and recovery of 7-aminodesacetoxycephalosporanic acid (7-ADCA) via 3-(carboxyethylthio)propionyl-7-ADCA, using a Penicillium chrysogenum...
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5780260 |
Immobilization of penicillin G amidase, glutaryl-7-ACA acylase or D-aminoacid oxidase on an aminofunctional organosiloxane polymer carrier
An enzyme selected from penicillin-G amidase, glutaryl-7-ACA acylase and D-amino acid oxidase is immobilized by covalent bonding on an aminofunctional organosiloxane polymer carrier to provide an...
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5773272 |
D-amino acid oxidase of F. solani and methods for its recombinant production
D-amino acid oxidase (DAO) originated from Fusarium solani M-0718, a DNA compound encoding the oxidase, expression vectors containing said DNA compound are provided. Esherichia coli transformants...
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5753458 |
Acylation method for penicillins and cephalosporins
A method for providing a semisynthetic β-lactam antibiotic by enzyme catalyzed acylation of the parent β-lactam with an activated derivative of the side chain acid wherein a modulator, which...
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5726032 |
Process for the efficient production of 7-ADCA via 2-(carboxyethylthio)acetyl-7-ADCA and 3-(carboxymethylthio)propionyl-7-ADCA
An overall efficient process for the preparation and recovery of 7-aminodesacetoxycephalosporanic acid (7-ADCA) via 2-(carboxyethylthio)acetyl- and 3-(carboxymethylthio)propionyl-7-ADCA, using a...
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5695951 |
Chlorination of cephalexin with chloroperoxidase from Rathayibacter biopuresis
An enzyme preparation that exhibits cephalosporin chloroperoxidase activity is isolatable from a microorganism species of the Rathayibacter genus. This enzyme preparation can convert cephalexin to...
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5677141 |
Process for producing 7-aminocephem compound or salts thereof
The present invention provides a process for producing 7-aminocephem compounds or salts thereof. 7-Aminocephem compounds are produced via microorganisms transformed with a vector containing a gene...
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5629171 |
Recombinant bioprocess for the preparation of 7-amino cephalosporanic acid (7-ACA)
Important intermediates for preparing cephalosporin antibiotics, 7-amino-cephalosporanic acid (7-ACA) and 7-aminodeacetylcephalosporanic acid (7-ADAC), are prepared by a novel bioprocess in which a...
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5618687 |
Production of 7-amino cephalosporanic acid with D-amino oxidase and deacylase
An enzymatic process for the production of 7-amino cephalosporanic acid from cephalosporine C is disclosed. The process is a two-stage enzymatic reaction which can be performed in a single reactor....
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5599702 |
D-amino acid oxidase from Trigonopsis variabilis immobilized on porous copolymer beads
D-amino acid oxidase (DAO) in purified, partially purified or crude form is immobilized on a porous bead-shaped support that is a copolymer of vinyl acetate and/or vinyl alcohol and a crosslinking...
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5597704 |
Bioconversion of cephalosporin C to glutaryl-7-aminocephalosporanic acid
A method of converting cephalosporin C to glutaryl-7-aminocephalosporanic acid. The method including obtaining a cell preparation from a microorganism which produces D-amino acid oxidase, the...
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5589354 |
Halogenation of cephalexin with haloperoxidase from Rathayibacter biopuresis
An enzyme preparation that exhibits cephalosporin haloperoxidase activity is isolatable from a microorganism species of the Rathayibacter genus. This enzyme preparation can convert cephalexin to a...
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5559006 |
Method for selectively deactivating catalase while retaining damino acid oxidase activity
"A method for selectively deactivating catalase while retaining D-amino acid oxidase activity is disclosed. The catalase and oxidase are both present in whole cells or a cell-free extract. The...
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5559005 |
Bioprocess for preparing 7-ACA and 7-ADAC
Important intermediates for preparing cephalosporin antibiotics, 7-amino-cephalosporanic acid (7-ACA) and 7-aminodeacetylcephalosporanic acid (7-ADAC), are prepared by a novel bioprocess in which a...
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5527697 |
7-amino-3-methoxymethyl-3-cephem-4-carboxylic ester hydrolase, process for its preparation, and its use
An esterase with a molecular weight of 55,200 ±300 Da is suitable for the chemoselective conversion of a compound of the formula II into a compound of the formula I. ##STR1##
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5512454 |
Enzymatic acylation of 3-hydroxymethyl cephalosporins
O-acylated cephalosporins are produced by reacting 3-hydroxymethylcephalosporins with an acyl donor containing at least three carbon atoms and an enzyme which is a Rodosporidium toruloides...
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5474912 |
Method for increasing production of microbial metabolites by genetic engineering
Targeted gene insertion methodology can increase the production of an antimicrobial metabolite and comprises the steps of: a) identifying the gene in unaltered chromosomal DNA which encodes an...
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5472853 |
Enzymatic process for cephalosporins
N-Terminal β,γ-didehydrovaline di- and tri-peptides are reacted with isopenicillin N synthetase to form 3-exomethylenecepham-4-carboxylic acids, e.g.,...
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