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5912236 |
Broad-spectrum tumor suppressor genes gene products and methods for tumor suppressor gene therapy
The present invention relates to a broad-spectrum tumor suppressor gene and the protein expressed by that gene in appropriate host cells. The protein is a second in-frame AUG codon-initiated...
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5910487 |
Cationic amphiphiles and plasmids for intracellular delivery of therapeutic molecules
Novel cationic amphiphiles are provided that facilitate transport of biologically active (therapeutic) molecules into cells. The amphiphiles contain lipophilic groups derived from steroids, from...
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5908777 |
Lipidic vector for nucleic acid delivery
A simple, rapid method for creating a lipidic vector for delivery of a therapeutic molecule entails bringing the molecule into contact with a polycation, thereby forming a complex, and then mixing...
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5906922 |
Delivery of nucleic acids
The present invention provides a method for introducing nucleic acids into cells. The method involves exposing the cells to a compound having formula (1), in which w is a nucleic acid, x is a...
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5891689 |
Heme-bearing microparticles for targeted delivery of drugs
Methods and compositions are described for the directed delivery of ribozymes or other compounds to specific cells which express the heme receptor on their surface using heme-bearing...
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5891717 |
Methods and compositions for inhibiting hexokinase
Disclosed are compositions and methods for inhibiting hexokinase enzymes in mammalian cells. Specifically provided are proteins that stimulate the production of trehalose-6-phosphate and their...
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5888821 |
Cholesterol derivative for liposomal gene transfer
The invention relates to a new cholesterol derivative for liposomal gene transfer. Areas of application of the invention are medicine and genetic engineering. The new cholesterol derivative,...
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5888764 |
Human fas gene promoter region
Disclosed is a 5' flanking sequence of the human fas gene containing a promoter region. This sequence also contains at least three transcription initiation sites, as well as consensus sequences for...
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5888983 |
Method and oligonucleobase compounds for curing diseases caused by mutations
The invention concerns methods of introducing specific alterations in the genome of cells that have been removed from a subject suffering from a medical condition that is the result of a genetic...
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5861314 |
Adeno-associated viral (AAV) liposomes and methods related thereto
A composition for genetic manipulation which comprises a liposome comprised of lipid material, and adeno-associated viral (AAV) material. Typically, the AAV material is plasmid, and comprises a...
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5861307 |
Human s-myc-like polypeptide and a gene coding for said polypeptide
Human s-myc-like gene, mycL2 (SEQ ID NO: 3) was isolated and identified from human placenta genomic DNA by PCR and human s-Myc-like polypeptide MycL2 (SEQ ID NO: 2) as the expression product of the...
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5858771 |
Products and methods for controlling the suppression of the neoplastic phenotype
A method for gene therapy for cancers wherein chromosomal location of an inactive or defective cancer suppressing gene is established, a replacement gene which is preferably cloned is then used to...
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5854038 |
Localization of a therapeutic agent in a cell in vitro
Method for enhancing the effect of a viral therapeutic agent in a cell in vitro on the viral target by tethering a localization signal to the therapeutic agent so that the agent localizes with the...
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5837507 |
Hox-induced enhancement of in vivo and in vitro proliferative capacity and gene therapeutic methods
Stem cells transduced with HOXB4 exhibit enhanced in vitro and in vivo ability for self-regeneration and generate higher-numbers of tranplantable pluripotent hematopoietic stem cells relative to...
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5827703 |
Methods and composition for in vivo gene therapy
Novel methods and compositions are provided for introducing a gene capable of modulating the genotype and phenotype into two or more tissues following systemic administration. The gene can be...
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5827705 |
Molecule and method for importing DNA into a nucleus
The invention provides a nuclear targeting molecule having a nucleic acid sequence which consists of SEQ ID NO:1 or a nuclear targeting portion of SEQ ID NO:1. The invention further provides a...
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5811406 |
Dry powder formulations of polynucleotide complexes
Polynucleotide complexes are stabilized by adding a cryoprotectant compound and lyophilizing the resulting formulation. The lyophilized formulations are milled or sieved into a dry powder...
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5811274 |
Methods, compositions and apparatus for cell transfection
This invention provides methods, compositions and apparatus for increasing the transfection efficiency of target cells by particles, especially retroviral particles, compared with that achieved by...
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5807836 |
Interferon regulatory factors 1 and 2 in the diagnosis of tumorigenicity
The present invention relates, in general, to a method of diagnosing tumorigenic mammalian cells or the propensity of a mammalian cell to become tumorigenetic. Additionally, the present invention...
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5792645 |
Protein-polycation nucleic acid complexes and methods of use
The invention relates to a system for transporting nucleic acids into the cell, which is effected by receptor-mediated endocytosis. Using a transferrin-polycation conjugate, a complex can be formed...
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5786214 |
pH-sensitive immunoliposomes and method of gene delivery to the mammalian central nervous system
This invention relates to pH-sensitive immunoliposomes with a conjugated antibody sensitive to cells of the mammalian CNS, a method for introducing genetic material into the cells of the mammalian...
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5783566 |
Method for increasing or decreasing transfection efficiency
Methods for controlling transfection efficiency mediated by complexes of cationic species and genetic material by adjusting the amount of membrane-associated proteoglycans and optionally adjusting...
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5776910 |
Methods of stimulating phagocytosis
The present invention relates, in general, to methods of stimulating phagocytosis and thereby combating infection and/or modulating immune complex disease, in particular, to methods of modulating...
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5766865 |
Cell lines capable of detecting low levels of cytokines in biological fluids
A method of genetically engineering a cell line capable of detecting bioactive cytokines or growth factors is provided. Cells lines produced by this method and methods of using these cell lines to...
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5759827 |
Acylated oligopeptides for transfecting cells
Compounds of the formula R 1 --NH--A I wherein R 1 is an acyl moiety of a C 12 -40 aliphatic carboxylic acid, A is the residue of a oligopeptide...
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5753477 |
Magneto-biolistic methods
The present invention discloses methods to transfect cells, comprising applying a strong magnetic field in pulses so as to affect a plurality of substance-carrying magnetic microparticles, the...
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5753262 |
Cationic lipid acid salt of 3beta N- (N', N'-dimethylaminoethane) - carbamoyl!cholestrol and halogenated solvent-free preliposomal lyophilate thereof
This invention discloses a novel cationic lipid acid salt of 3β N-(N',N'-dimethylaminoethane)-carbamoyl!cholesterol. This invention further discloses a transmembrane compatible body suitable for...
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5750376 |
In vitro growth and proliferation of genetically modified multipotent neural stem cells and their progeny
A method for producing genetically modified neural cells comprises culturing cells derived from embryonic, juvenile, or adult mammalian neural tissue with one or more growth factors that induce...
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5747340 |
Targeted gene expression using preproendothelin-1 promoters
This invention provides a vector for expression of a nucleic acid cassette in bronchial epithelial and vascular endothelial cells comprising a segment of the 5'-flanking region of the...
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5736395 |
Amphiphilic imidazolinium derivatives
Amphiphiles containing an imidazdinium ring system are provided that are non-toxic to the host mammal. The amphiphiles are used to produce liposomes useful as carriers for delivering macromolecules...
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5736360 |
Endothelial cell tropic compositions and methods of making and using the same
An isolated nucleic acid molecule comprising a modified Murine Leukemia Virus Long Terminal Repeat sequence that is capable of tropically regulating expression of operably linked coding sequences...
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5733762 |
Complexes of nucleic acid and polymer, their process of preparation and their use for the transfection of cells
A compound consisting essentially of polylysine conjugated to non-charged residues and recognition signals wherein the free amino functions of said polylysine are substituted with non-charged...
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5705151 |
Gene therapy for T cell regulation
The present invention provides a nucleic acid-based therapeutic composition to treat an animal with disease by controlling the activity of effector cells, including T cells, macrophages, monocytes...
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5698427 |
Methods and compositions involved in cell growth, neoplasia and immunoregulation
A peptide, Hel-N1, which can bind to a 3'-untranslated mRNA sequence (which encompasses the "instability sequence") that is uniquely present in the messenger RNAs that encode oncoproteins and...
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5679559 |
Cationic polymer and lipoprotein-containing system for gene delivery
A composition for delivery of a selected nucleic acid into a targeted host cell comprises a complex of a hydrophobized, positively charged, biocompatible polymer; a lipoprotein; and a selected...
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5676954 |
Method of in vivo delivery of functioning foreign genes
A method of expressing a foreign gene in cells of a mammalian organ in vivo includes the step of injecting genetic material capable of inducing desired gene products into a mammal. The genetic...
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5641662 |
Transfection of lung via aerosolized transgene delivery
Methods and compositions for producing a mammal capable of expressing an exogenously supplied gene in cells of the airway are disclosed. Lipid carrier-nucleic acid complexes are prepared then...
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5641660 |
Glutamicum threonine biosynthetic pathway
The present invention is a method for the isolation and characterization of C. glutamicum genes involved in amino acid biosynthesis, specifically, encoding hom, thrB, and thrC, and sequences...
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5635382 |
Method for enhancing transmembrane transport of exogenous molecules
A method is provided for enhancing transmembrane transport of exogenous molecules. The method comprises contacting a membrane of a living cell with a complex formed between said molecules and...
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5635383 |
Method for the introduction of genes into mammalian cells by a soluble molecular complex comprising a receptor ligand and a polycation
A targetable gene delivery system is provided for introducing foreign genes into mammalian cells. The system employs a soluble targetable DNA complex and utilizes receptor-mediated endocytosis to...
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5635380 |
Enhancement of nucleic acid transfer by coupling virus to nucleic acid via lipids
This invention provides a method of enhancing the delivery of a nucleic acid into a cell, wherein a complex is formed comprising the nucleic acid, a cationic liposome, and a replication-deficient...
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5629184 |
Cationic copolymers of vinylamine and vinyl alcohol for the delivery of oligonucleotides
The present invention relates to an improved method for the cytoplasmic delivery of oligonucleotides using cationic copolymers based on vinyl alcohol with low levels of vinyl amine (PVAVAMs)....
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5627159 |
Enhancement of lipid cationic transfections in the presence of serum
A method is provided for enhancing transfection efficiency of eukaryotic cells comprising contacting said cells with a lipid aggregate comprising nucleic acid and a polycationic lipid composition...
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5614397 |
Method and compositions for modulating lifespan of hematolymphoid cells
Methods and compositions for modifying the lifespan of progeny cells of mammalian hematopoietic stem cells, particularly myeloid series cells, are provided. Transgenic nonhuman mammals also are...
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5614503 |
Amphipathic nucleic acid transporter
A nucleic acid transporter to deliver a nucleic acids into cells, comprising a cationic compound having a cationic head group for binding the nucleic acid and a lipid tail for association with the...
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5597689 |
Nucleotide sequences permitting the replication and maintenance of a genetic information in animal cells
The subject of the invention is the use of a DNA fragment comprising a sequence from the 3' end of the AcNPV baculovirus IE1 gene, optionally combined with a DNA fragment comprising a sequence...
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5595899 |
Methods of introducing foreign DNA into cells
The present invention provides an easy and efficient method of introducing a foreign DNA into certain cells which have been considered difficult to be transformed by conventional methods. The...
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5595897 |
Polylysine conjugates
The invention concerns a complex between at least one negatively charged nucleic acid and at least one positively charged polymeric conjugate, the link between the nucleic acid and the polymeric...
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5583020 |
Permeability enhancers for negatively charged polynucleotides
This invention features permeability enhancer molecules, and methods, to increase membrane permeability of negatively charged polymers thereby facilitating cellular uptake of such polymers.
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5552309 |
Use of polyols for improving the introduction of genetic material into cells
A process for introducing an expression vehicle (e.g., plasmids, retroviral vectors, adenoviral vectors) into cells, which comprises contacting the cells with the expression vehicle and a polyol....
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