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7241588 |
Method for producing novel β-lactam antibiotic from protoplast fusion strain
The present invention provides a method for producing a novel β-lactam antibiotic from a protoplast fusion strain. The method is to fermentatively culture the protoplast fusion strain of ...
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7229784 |
Modulation of secondary metabolite production by zinc binuclear cluster proteins
Methods for improving the production of a secondary metabolite by a fungus by increasing the yield or productivity of the secondary metabolite produced by the fungus are described. The methods...
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7053204 |
Global regulator of secondary metabolite biosynthesis and methods of use
The invention provides polypeptides and nucleic acids which identify and encode LaeA, a regulator of fungal secondary metabolite production which exhibits global control over secondary metabolite...
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6974675 |
Process for identifying and enriching cell-specific target structures
This invention relates to a process for identifying and enriching cell-specific target structures, in particular for the identification of cell-specific protein combination patterns on the surface...
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6949356 |
Methods for improving secondary metabolite production in fungi
The invention relates to the production of secondary metabolites by fungi. More particularly, the invention relates to improvement of production of commercially important secondary metabolites by...
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6924115 |
Process for identifying cell-specific target structures
This invention relates to a process for identifying cell-specific target structures. The process comprises automatically depositing a reagent solution that includes at least one marker molecule on...
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6642035 |
Synthesis of B-keto esters
β-keto esters are prepared by way of a lipase-catalyzed transesterification. The synthetic methodology provides a simple scheme for the synthesis of optically active β-keto esters that are useful...
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6518039 |
Process for the fermentative production of deacylated cephalosporins
The present invention discloses a process for the production of N-deacylated cephalosporin compounds via the fermentative production of their 7-acylated counterparts.
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6503727 |
Process for the preparation of an antibiotic
The invention relates to a process for the preparation of an antibiotic, in particular cefalexin, ampicillin, amoxicillin, cefaclor, cefradin, cefadroxil, cefotaxim and the like, wherein a...
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6383772 |
Enzymatic conversion in a solvent mixture containing water and flourinated, non-chlorinated solvent
A first compound is converted to a second compound enzymatically in a solvent mixture containing water and a fluorinated, non-chlorinated alkane, alkene, or alkyne having up to 4 carbon atoms....
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6379922 |
Membrane enzyme reactor containing a plurality of biocatalyst-immobilized sheets
An enzyme membrane reactor is designed to enhance the production efficiency of foods, pharmaceutical products, and other bioproducts. The reactor is formed of a cap and a container in which a...
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6361982 |
Regulatory gene for clavulanic acid biosynthesis
DNA comprising a regulatory gene for clavulanic acid biosynthesis. DNA is no greater than 7 kb in length and has the configuration of restriction sites shown in FIG. 1 or a fragment thereof. Use...
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6317795 |
Dynamic modification of multimedia content
A method and system for manipulating or modifying identifiable objects in a standard broadcast or Internet-based multimedia stream according to a control specification and a content specification....
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6287799 |
Process for the preparation of aβ-lactam antibiotic
Process for the preparation of a β-lactam antibiotic in which a β-lactam nucleus is subjected to an enzymatic acylation reaction with the aid of an acylation agent at a molar ratio of acylation...
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6258555 |
DNA encoding ACV synthetase
DNA encoding the gene for the synthetase enzyme capable of generating δ (L-a-aminoadipyl)-L-crysteinyl-D-valine (ACV) from its constituent amino acids was obtained from several penicillin and...
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6251655 |
Process for increasing the production of penicillin G (benzylpenicillin) in Penicillium chrysogenum by expression of the PCL gene
An isolated DNA encoding phenyl acetyl-CoA-ligase and a process of increasing the production of penicillin G in a strain of Penicillium chrysogenum by transforming the strain with the isolated DNA....
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6218138 |
Synthesis of beta-lactam antibiotics with immobilized penicillin amidase
Beta-lactam antibiotics are synthesized by reacting an amino-beta-lactam component with a corresponding amino-group-containing acylating side-chain component in the presence of penicillin amidase...
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6180360 |
Process of producing β-lactam antibiotics applying micro-organisms with increased ligase activity
The present invention relates to the biosynthesis of β-lactam antibiotics. More specifically, the invention relates to processes of producing β-lactam antibiotics in vivo and in vitro. Also...
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6159706 |
Application of enzyme prodrugs as anti-infective agents
The present invention provides a method for targeting toxic antimetabolites to gram negative infections. It provides a means of taking advantage of a key disease resistance mechanism to activate...
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6156534 |
Synthesis of β-lactam antibacterials using soluble side chain esters and enzyme acylase
Disclosed is a process for the synthesis of β-lactam antibacterials using soluble side chain esters in the presence of enzyme acylase. Also disclosed are novel esters useful as reactants in said...
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6110699 |
Process for producing 6-amino-penicillanic acid and phenylacetic acid
Alternative process for obtaining 6-aminopenicillanic acid. The process comprises replacing the stages of extraction with organic solvents and isolation and separation of the intermediate...
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6100052 |
Process for the production of clavulanic acid and/or salts thereof by fermentation controlling soluble phosphate concentration
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6020151 |
Process for the production of 7-ADCA via expandase activity on penicillin G
A process is taught for the preparation and recovery of 7-aminodesacetoxycephalosporanic acid (7-ADCA) via enzymatic ring expansion activity on penicillin G, using a Penicillium chrysogenum...
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5942411 |
Process of producing B-lactam antibiotics applying microorganisms with increased ligase activity
The present invention relates to the biosynthesis of β-lactam antibiotics. More specifically, the invention relates to processes of producing β-lactam antibiotics in vivo and in vitro. Also...
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5922907 |
Precursors of β-lactam antibacterials having soluble side chain esters
Disclosed is a process for the synthesis of β-lactam antibacterials using soluble side chain esters in the presence of enzyme acylase. Also disclosed are novel esters useful as reactants in said...
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5916762 |
Process for the recovery of ampicillin
Process for the recovery of ampicillin from a mixture containing ampicillin and 6-aminopenicillic acid (6-APA), in which a mixture of ampicillin and 6-APA, with a pH higher than 7, which apart from...
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5882883 |
Process for the production of secondary metabolites
The invention relates to an improved process for the production of a secondary metabolite comprising (i) fermentation of a microorganism capable of producing said secondary metabolite, and (ii)...
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5882879 |
Method for influencing β-lactam antibiotic production and for isolation of large quantities of ACV synthetase
Novel methods and compositions are provided for the enhanced production of β-lactam antibiotics. The process is exemplified by the production of penicillin. In addition, the P. chrysogenum and A....
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5801011 |
Process for preparing cephalosporins with penicillin acylase without pH control
The invention relates to an improved process for preparing cephalosporins by reaction compounds such as 7-amino-cephalosporanic acid, 7-amino-3-deacetoxy-cephalosporanic acid or their derivatives...
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5780260 |
Immobilization of penicillin G amidase, glutaryl-7-ACA acylase or D-aminoacid oxidase on an aminofunctional organosiloxane polymer carrier
An enzyme selected from penicillin-G amidase, glutaryl-7-ACA acylase and D-amino acid oxidase is immobilized by covalent bonding on an aminofunctional organosiloxane polymer carrier to provide an...
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5695968 |
Process for production of D-α-amino acids
The present invention is directed to a gene which is related to a D-N-carbamoyl-α-amino acid amidohydrolase which is an enzyme capable of converting D-N-carbamoyl-α-amino acids into D-α-amino...
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5621096 |
Process for preparing penems
The invention provides a process for the preparation of the compound of formula I ##STR1## wherein R is carboxy group or a carboxylate anion, by enzymatic hydrolysis with a lipase or an acylase in...
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5521068 |
Process for 7-aminodesacetoxycephalosporanic acid
The invention relates to an improved process for converting 6-acylaminopenicillanic acid (6-AAPA) to 6-aminopenicillanic acid (6-APA). The process employs a solution or suspension of penicillin...
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5474912 |
Method for increasing production of microbial metabolites by genetic engineering
Targeted gene insertion methodology can increase the production of an antimicrobial metabolite and comprises the steps of: a) identifying the gene in unaltered chromosomal DNA which encodes an...
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5472853 |
Enzymatic process for cephalosporins
N-Terminal β,γ-didehydrovaline di- and tri-peptides are reacted with isopenicillin N synthetase to form 3-exomethylenecepham-4-carboxylic acids, e.g.,...
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5468632 |
Recombinant DNA compounds and expression vectors encoding para-nitrobenzyl esterase activity from bacillus
The present invention provides isolated DNA compounds and recombinant DNA cloning and expression vectors that encode PNB esterase from Bacillus subtilis. The invention also provides host cells...
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5462862 |
Method and compositions for enhancing production of secondary metabolites using clustered biosynthetic genes
Clustered antibiotic biosynthetic genes are employed for improvement of production of the antibiotic in microorganisms and for the isolation of other genes involved in the biosynthesis of the...
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5407870 |
Process for fabricating a semiconductor device having a high reliability dielectric material
A process for fabricating a high-reliability composite dielectric layer (19) includes the formation of a first oxynitride layer (14) on the surface (12) of a silicon substrate (10). The formation...
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5364768 |
Process for the preparation of penems
An in vitro process for preparing 6((1R)-hydroxyethyl) penem acids by hydrolyzing the carboxylic ester derivative thereof using an enzyme capable of selectively hydrolyzing the ester group at the...
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5340483 |
Two step process for conversion of a weakly adsorbable compound to a strongly adsorbable compound and selective removal thereof
A method for the selective separation of a particular compound from a mixture of compounds, by enzymatically converting a weakly adsorbable compound to a strongly adsorbable compound, and binding...
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5268271 |
Method for the synthesis of semi-synthetic antibiotics in thermodynamically controlled water-cosolvent organic miscible apolar systems by using penicillin G acylase
Synthesis of semi-synthetic monobactamic or β-Lactamic antibiotics by using derivatives stabilized by various methods of penicillin G acylase from various microbial sources according to a...
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5215891 |
Process for preparing penems
A process for preparing a compound of formula I: ##STR1## wherein R 1 represents a hydroxy protecting group and R 2 represents a carboxy protecting group, which comprises: hydrolyzing a...
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5079146 |
Method of protection of the carboxy groups in the chemistry of β-lactam compounds
The present invention relates to a new method of protection of the carboxy group in the chemistry of the compounds of β-lactam type. According to such a method, the carboxy group is protected by...
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5013452 |
Resolution of emulsions formed in the production of pharmaceuticals
A method for resolving emulsions produced in preparation of pharmaceuticals by fermentation and in other such bioprocesses is disclosed. The method comprises adding an effective amount of a...
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4980284 |
Method of preparing D-α-amino acids
A substantial elevation of the space-time yield is achieved in the preparation of D-α-amino acids by means of the biotransformation of hydantoins which are monosubstituted in the 5-position in an...
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4980282 |
Treatment of corn steep liquor
A process for the treatment of corn steepwater. The steepwater is incubated for a sufficient time to develop biomass before corn steepwater is fed continuously to the developed biomass at a rate...
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4865973 |
Process for extractive fermentation
A process for producing a variety of chemical products, e.g., ethanol, by fermentation in which the product is removed from the fermentation medium as it is formed by liquid-liquid extraction using...
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4847200 |
Biosynthesis of unnatural cephalosporins
A process for producing unnatural penicillins and cephalosporin derivatives thereof in which peptide analogs of ACV in which the L-α-aminodipyl moiety is replaced by L-S-carboxymethyl cysteine or...
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4769325 |
2-(Allenyl)penicillins
2-(Allenyl)penicillins antibiotics and intermediates thereto are disclosed. A process for making the 2-(allenyl)penicillins and the starting materials for the process are also disclosed.
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4732851 |
Immobilization of cells with a polyazetidine prepolymer
A composition comprising immobilized cells obtained by applying a dispersion of cells and curable prepolymer material selected from the group consisting of polyazetidine prepolymers, carboxymethyl...
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