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8138147 |
Conditioned cell culture medium compositions and methods of use
Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known...
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8017390 |
Method for the preparation of dermal papilla tissue employing mesenchymal stem cells
A method for the preparation of dermal papilla tissue comprising the step of culturing mesenchymal stem cells in a medium having a specific composition is provided. The method makes it possible to...
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7923245 |
Medium for ES culturing
The present invention discloses a medium for a serum-free medium capable of culturing ES cells for a long period while maintaining their undifferentiated state without using feeder cells, and a...
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7790458 |
Material and methods for the growth of hematopoietic stem cells
The invention provides populations of expanded CD34-expressing cells and methods of use. Particular embodiments provide for defined culture media useful for growing these cells, and grafts...
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7723106 |
Stroma-free, serum-free, and chemically defined medium and method for ex vivo mononuclear cell expansion using the same
A stroma-free, serum-free, and chemically defined medium and a method for mononuclear cell expansion ex vivo using the same. An exemplary medium includes a basal medium, a serum substitute, and a...
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7691629 |
Transplantation of human neural cells for treatment of neurodegenerative conditions
A method of treating neurodegenerative conditions is provided. Neural stem cells may be implanted at and/or remote from a region of neuron degeneration. The methods can include isolating neural...
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7635589 |
Method for the preparation of a dermal papilla tissue having hair follicle inductive potency
The inventive method for the preparation of a dermal papilla tissue in accordance with the present invention makes it possible to form a quantity of the dermal papilla tissues having hair follicle...
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7300794 |
Accelerated culture system for intestinal epithelial cell monolayers
The present invention provides a unique medium for the cultivation of intestinal cell lines. The medium allows for the development of a highly differentiated intestinal epithelial cell monolayer in...
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7192768 |
Synchronization of the cytoplasmatic and the nuclear maturation of oocytes in vitro
The present invention relates to a method for in vitro maturation of oocytes comprising the steps of: (a) culturing one or more GV oocytes in a culture medium, the culture medium comprising a...
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7169610 |
Serum-free media for chondrocytes and methods of use thereof
The present invention provides defined serum-free cell culture media useful in culturing fibroblasts, especially articular chondrocytes, that avoids problems inherent in the use of serum-containing...
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7132288 |
Medium for culturing tumor cells
Disclosed is a medium for cultivating tumor cells, comprising the following constituents, as comprised for example in the medium RPMI 1640: (a) fetal calf serum (FCS), (b) penicillin-streptomycin,...
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6998266 |
Active retinoic acid-free culture medium for avian totipotent embryonic stem cells
A culture medium for avian embryonic cells and an avian cell culture medium is disclosed. The culture medium is characterized in that it has elements complementary to avian embryonic cells. The...
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6946241 |
Physiological medium for perfusing, preserving and storing isolated cell, tissue and organ samples
A physiological liquid medium is provided having the basic, synergistic components to allow its universal application in preserving cellular and functional integrity in vitro of different cell,...
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6900056 |
Chemically defined medium for cultured mammalian cells
The present invention relates to methods and compositions for chemically defined media for growth of mammalian cells for production of commercially useful amounts of expressed proteins.
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6838284 |
Cell culture medium containing growth factors and L-glutamine
The invention relates to a culture medium for culturing cells, in particular human cells in a process for tissue engineering bone. The medium comprises glucose, a mineral, a vitamin, a growth...
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6733746 |
Hematopoietic cell culture nutrient supplement
The present invention provides a serum-free supplement which supports the growth of hematopoietic cells in culture. Also provided are a medium comprising a basal medium supplemented with the...
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6709864 |
Adipogenic differentiation of human mesenchymal stem cells
A composition which comprises human mesenchymal stem cells which have the potential to differentiate into cells of more than one connective tissue type and a composition which induces cells from...
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6706683 |
Method for controlling the release of granules
A method of controlling granule secretion comprising performing a treatment to increase or decrease an active form of calgranulin on a cell line having capability of secreting granules, thereby...
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6692961 |
Defined systems for epithelial cell culture and use thereof
The present invention provides cell culture media formulations which support the in vitro cultivation of animal epithelial cells. The media comprise at least one fibroblast growth factor (FGF) and...
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6599740 |
Method of using fish plasma components for tissue culture
A process of using a fish plasma component as a nutrient medium component for tissue culture includes obtaining blood from a fish, separating plasma from the blood, and extracting one or more...
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6562620 |
Medium to promote islet cell survival
The present invention relates to a medium for promoting the survival of islet cells, which comprises one or more growth factors in combination with FK506 in amounts having an anti-apoptotic effect...
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6506576 |
Serum-and steroid-free culture media for cerebellar granule neurons
The invention is a system for maintenance and high-throughput analysis of cerebellar granule neurons in tissue culture plates under chemically defined conditions. The invention includes serum-free...
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6413772 |
Cell structure media for mammalian cells
A chemically defined mammalian cell culture medium is provided that supports maintenance and long term clonal growth of mammalian hepatocytes and other cells.
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6355237 |
Methods for using the obese gene and its gene product to stimulate hematopoietic development
The present invention relates to methods for using various forms of a novel receptor expressed by hematopoietic and endothelial cells. An additional variant form of this receptor has been detected...
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6326194 |
Method for providing cell growth
Growth of keratinocytes is provided by subjecting a basal medium, insulin, pituitary compound and keratinocytes to cell growth conditions. Cell growth can be provided in the absence of exogenous...
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6274305 |
Inhibiting proliferation of cancer cells
A method of testing cancer cells is described. Assays are provided for determining the potential for inhibiting cancer cells proliferation using albumin-derived peptides. The methods of the present...
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6180404 |
Cultural medium for maintaining neural cells in ambient atmosphere
The present invention provides a minimal essential medium for maintaining neural cell or tissue viability in an environment containing ambient levels of CO2. The medium contains less than about...
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6171825 |
Preparation of recombinant factor VIII in a protein free medium
Recombinant Factor VIII can be produced in relatively large quantities on a continuous basis from mammalian cells in the absence of any animal-derived proteins such as albumin by culturing the...
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6159462 |
Uses of Wnt polypeptides
Uses for Wnt polypeptides in hematopoiesis are disclosed. In particular, in vitro and in vivo methods for enhancing proliferation, differentiation or maintenance of a hematopoietic stem/progenitor...
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6153432 |
Methods for the differentiation of human preadipocytes into adipocytes
The present invention provides methods and compositions for the consistent and quantitative differentiation of human preadipocytes isolated from adipose tissue into adipocytes bearing biochemical,...
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6150163 |
Chondrocyte media formulations and culture procedures
One object of the present invention is based upon the development and use of a serum-free defined cell culture medium comprising a supplement mixture, a component mixture, a vitamin mixture, an...
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6103523 |
Pluripotent rabbit cell lines and method of making
The present invention relates to a rabbit embryonic stem (ES) cell line, comprising at least 70%, preferably 80 to 90% undifferentiated cells and obtainable by isolating the inner cell mass of 5.5...
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6087174 |
Growth medium for primary pancreatic tumor cell culture
The present invention provides a method of treating cancer comprising (a) obtaining a tumor cell line, (b) modifying the tumor cell line to render it capable of producing an increased level of a...
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6054317 |
System for the cell culture and cryopreservation of marine invertebrates
A system for the culture of eukaryotic cells of marine invertebrates employing a culture medium containing a mixture of sodium, magnesium, chlorine, potassium, calcium, bromine, and sulfate is...
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6037175 |
Method and media for enhancing cryopreservation of cells
A method of enhancing the viability of cryopreserved cells is culturing Sertoli cells in media to produce preconditioned media and adding the preconditioned media to the cells to be cryopreserved....
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6027937 |
Method for propagation of insecta germ cells
A method is disclosed for the in vitro growth and proliferation of germ cells obtained from the ovarioles of an insect. By culturing these cells in a medium supplemented with soluble cytokines and...
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5985665 |
Biochemical analysis of antioxidant function of lymphocytes in culture
The present invention provides a cell culture medium useful for a biochemical analysis of antioxidant function in human lymphocytes, said medium comprising, a buffered, serum-free solution...
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5985290 |
Purified pancreatic tumor cell lines and related compositions and method
The present invention provides a method of treating cancer comprising (a) obtaining a tumor cell line, (b) modifying the tumor cell line to render it capable of producing an increased level of a...
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5952301 |
Compositions and methods for enhancing intestinal function
GLP-2 stimulates the growth of both small intestine and large intestine tissue when administered in conjunction with other peptide hormones. The invention provides pharmaceutical compositions of...
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5916809 |
Medium for culturing normal human epidermal melanocytes
Provided is a medium for culturing normal human epidermal melanocytes in vitro. The medium comprises a basal medium for culturing animal cells, Ca2+ at a final concentration of between about 0.15...
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5910443 |
Medium for culturing human olfactory neurons
The present invention relates to a culture of human olfactory neurons. The neurons may display a normal neuronal pathology or a pathology characteristic of a generalized central nervous system...
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5902741 |
Three-dimensional cartilage cultures
The present invention relates to a method of stimulating the proliferation and appropriate cell maturation of a variety of different cells and tissues in three-dimensional cultures in vitro using...
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5888705 |
Compositions and method of stimulating the proliferation and differentiation of human fetal and adult pancreatic cells ex vivo
A method of inducing the proliferation and/or differentiation of human adult pancreatic cells entails contacting primary cultures of such cells with Hepatocyte Growth Factor/Scatter Factor...
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5874306 |
Culturing human pancreatic endocrine cells in medium containing extracellular matrix from human bladder carcinoma cells
Human pancreatic endocrine cells are proliferated without loss of hormone function in a culture medium containing extracellular matrix from bladder carcinoma cell lines in the substantial absence...
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5846807 |
Media compositions for three-dimensional mammalian tissue growth under microgravity culture conditions
Normal mammalian tissue and the culturing process has been developed for the three groups of organ, structural and blood tissue. The cells are grown in vitro under microgravity culture conditions...
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5837543 |
Human embryo co-culture system and uses thereof
The present invention provides a human embryo co-culture system comprising a suspension of cultured human tubal epithelial cells. Also provided is a method of method of improving the pregnancy rate...
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5814511 |
Human breast epithelial cell type with stem cell and luminal epithelial cell characteristics
Described is a substantially purified human breast epithelial cell (Type I HBEC) displaying the following characteristics: variable cell shape; smooth cell colony boundary; deficiency in gap...
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5780301 |
Serum-free medium for cultivation of postnatal central neurons
Disclosed is a synthetic medium with PDGF, vitronectin, IL-1β and BSA added to Eagle's minimum essential medium or medium with transferrin, insulin, progesterone and putrescine further added ...
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5766951 |
Serum-free medium supporting growth and proliferation of normal bone marrow cells
A serum-free medium which supports the growth and proliferation of bone marrow cells is described. Recipes for two formulations are given, one of which provides a medium suitable for growth of bone...
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5744361 |
Expansion of human hematopoietic progenitor cells in a liquid medium
The use of individual or combinations of cytokines, particularly IL-3, GM-CSF, and c-kit ligand are employed for long-term hematopoiesis in serum free culture in the absence of stromal cells. The...
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