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6372210 |
Method for repopulating human bone marrow comprising culturing CD34&plus cells in a serum free medium
A serum-free medium which supports the proliferation and differentiation of CD34 + cells purified from normal bone marrow, peripheral blood of patients treated with cytokines, and umbilical cord...
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6365385 |
Methods of culturing and encapsulating pancreatic islet cells
Cells, preferably isolated pancreatic islet cells, are treated with a compound or a combination of compounds selected from an antioxidant, an anti-cytokine, an anti-endotoxin and an antibiotic. The...
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6338964 |
Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration
A cell culture medium which is low in dissolved carbon dioxide is disclosed. The medium contains less than about 1 g/L added sodium bicarbonate and includes an organic buffer and a metal complexing...
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6335196 |
13C,15N ,2H labeled proteins for NMR structure determinations and their preparation
This invention is concerned with determining the three-dimensional structure of biological macromolecules such as proteins. In particular, it is concerned with methods for rapidly determining...
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6333193 |
Cell growth accelerator and cell growth method using the same
Provided are a cell growth accelerator containing a polyphosphoric acid and a cell growth method in which cells are grown by adding the cell growth accelerator to a medium for cells of animals and...
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6326194 |
Method for providing cell growth
Growth of keratinocytes is provided by subjecting a basal medium, insulin, pituitary compound and keratinocytes to cell growth conditions. Cell growth can be provided in the absence of exogenous...
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6323025 |
In vitro cell culture in media containing beta-alanyl-taurine or carbobenzoxy beta-alanyl-taurine
Compounds termed "vitaletheine modulators" which include beta-alanyl-taurine and carbobenzoxy beta-alanyl-taurine are synthesized and added to culture media for in vitro culture of cells such as...
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6309862 |
Methionine containing animal cell culture medium and its use
A nutrient medium for protein producing cells, characterized in that said medium consists essentially of the following components: a physiological saline containing Ca 2 +, K + , Mg 2 + and Na + ,...
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6303355 |
Method of culturing, cryopreserving and encapsulating pancreatic islet cells
Cells, preferably isolated pancreatic islet cells, are treated with a compound or a combination of compounds selected from an antioxidant, an anti-cytokine, an anti-endotoxin and an antibiotic. The...
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6274348 |
Methods for the preparation of positively charged proteins
Disclosed are novel hybrid fusion proteins comprising at their amino terminus, bactericidal/permeability-increasing protein or a biologically active fragment thereof and, at their carboxy terminus,...
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6265180 |
Nanoemulsions for delivering lipophilic substances into cells
Sterile nanoemulsions are prepared containing oily droplets of a diameter of less than 100 nm in an aqueous phase. The oily droplets contain a lipophilic substance, and have on their surface an...
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6251670 |
Method of culturing cells in suspension using lectins
An object of the present invention is to provide a method of enabling perfusion culture efficiently and simply by agglutinating cells with Lectin, which is a naturally-occurring agglutinin, thereby...
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6248588 |
Medium for preserving biological materials
The present invention relates to a medium allowing the preservation and cryopreservation of biological materials such as animal cells and viral particles that are directly injectable or...
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6228606 |
Culture medium for detecting pathogenic bacteria of the genus Listeria and method for identifying said bacteria
The present invention relates to a nutritive agar culture medium allowing the direct identification of pathogenic bacteria of the genus Listeria, said medium comprising a chromogenic synthetic...
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6210966 |
Culture medium for insect cells lacking glutamine an contains ammonium salt
A culture medium for growth of insect cells, such as Spodoptera frugiperda, is provided. The culture medium lacks glutamine, and may optionally lack glutamate and/or aspartate. Further the insect...
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6210965 |
Cell culture process and medium, cellular composition obtained and its application as production system and study model
The invention relates, inter alia, to a process for in vitro culture of any cells whatsoever, of human or animal origin. The process's characterizing feature is that it involves at least a stage of...
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6180404 |
Cultural medium for maintaining neural cells in ambient atmosphere
The present invention provides a minimal essential medium for maintaining neural cell or tissue viability in an environment containing ambient levels of CO 2 . The medium contains less than about...
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6171825 |
Preparation of recombinant factor VIII in a protein free medium
Recombinant Factor VIII can be produced in relatively large quantities on a continuous basis from mammalian cells in the absence of any animal-derived proteins such as albumin by culturing the...
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6168810 |
Method and culture for producing cheese and other dairy products
The present invention relates to a method for preparing cheese and other dairy products and more specifically to methods for preparing cheese and other dairy products from secondary milk products...
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6162643 |
Hepes based basal nutrient medium for the isolation and culturing of stem cells
Novel media and methods are disclosed for the growth of stem cells. The media are serum-free, companion cell or feeder layer free and organotypic, matrix free solutions for the isolation and...
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6159462 |
Uses of Wnt polypeptides
Uses for Wnt polypeptides in hematopoiesis are disclosed. In particular, in vitro and in vivo methods for enhancing proliferation, differentiation or maintenance of a hematopoietic stem/progenitor...
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6153432 |
Methods for the differentiation of human preadipocytes into adipocytes
The present invention provides methods and compositions for the consistent and quantitative differentiation of human preadipocytes isolated from adipose tissue into adipocytes bearing biochemical,...
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6150163 |
Chondrocyte media formulations and culture procedures
One object of the present invention is based upon the development and use of a serum-free defined cell culture medium comprising a supplement mixture, a component mixture, a vitamin mixture, an...
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6150345 |
Methods for promoting survival of myelin producing cells
The present invention provides a method for promoting the survival of myelin producing cells, in particular SCs and oligodendrocytes. Other embodiments of the present invention are directed to...
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6150459 |
Comb polymers for regulating cell surface interactions
Synthetic comb copolymers which elicit controlled cellular response, methods of applying these polymers to various surfaces, and methods of using the polymers for modifying biomaterial surfaces, in...
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6140123 |
Method for conditioning and cryopreserving cells
A method is provided for preconditioning and cryopreservation of cells harvested from a donor. Cells are suspended in a cell conditioning and cryopreservation medium containing a cryopreservative...
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6136600 |
Method for cultivation of hepatocytes
The present invention provides a method for cultivation of hepatocytes efficiently by using a hepatocyte growth promoting factor derived from 3T3 cells, which comprises culturing hepatocytes...
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6130086 |
Composition for an in vitro fertilization medium
The present invention aims to provide a medium composition for in vitro fertilization, in particular, a composition usable in the culture of ova or early embryos which are fertilized eggs, the...
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6110504 |
Plasma-like substance
An artificial plasma-like substance having at least one water soluble polysaccharide oncotic agent selected from the group consisting of high molecular weight hydroxyethyl starch, low molecular...
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6103528 |
Reversible gelling culture media for in-vitro cell culture in three-dimensional matrices
A gelling cell culture medium useful for forming a three dimensional matrix for cell culture in vitro is prepared by copolymerizing an acrylamide derivative with a hydrophilic comonomer to form a...
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6103529 |
Animal cell culture media comprising peptides derived from rice
The present invention provides serum-free cell culture media formulations which are capable of supporting the in vitro cultivation of animal cells. The media comprise at least one nutrient of plant...
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6100061 |
Recombinant cell clone having increased stability in serum- and protein-free medium and a method of recovering the stable cell clone and the production of recombinant proteins by using a stable cell clone
Disclosed are a stable recombinant cell clone which is stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and...
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6087174 |
Growth medium for primary pancreatic tumor cell culture
The present invention provides a method of treating cancer comprising (a) obtaining a tumor cell line, (b) modifying the tumor cell line to render it capable of producing an increased level of a...
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6054311 |
Recovering cryopreserved cells using gassed polyfluorinated compounds
After the cryopreservation of living cells, the cells in a post-thaw recovery stage are supported by a gas permeable medium. This medium comprises at least one polyfluorinated compound. This...
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6054317 |
System for the cell culture and cryopreservation of marine invertebrates
A system for the culture of eukaryotic cells of marine invertebrates employing a culture medium containing a mixture of sodium, magnesium, chlorine, potassium, calcium, bromine, and sulfate is...
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6048728 |
Cell culture medium for enhanced cell growth, culture longevity, and product expression
Protein-free cell culture media supplements are described consisting of synergistic combinations of medium components, which when added to cell culture media, either serum supplemented or...
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6037175 |
Method and media for enhancing cryopreservation of cells
A method of enhancing the viability of cryopreserved cells is culturing Sertoli cells in media to produce preconditioned media and adding the preconditioned media to the cells to be cryopreserved....
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6037174 |
Preparation of serum-free suspensions of human hematopoietic cells or precursor cells
Provided are serum-free, animal protein-free media formulations to be used in conjunction with hematopoietic growth factors for the in vitro growth of human neutrophil and megakaryocyte precursors....
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6027937 |
Method for propagation of insecta germ cells
A method is disclosed for the in vitro growth and proliferation of germ cells obtained from the ovarioles of an insect. By culturing these cells in a medium supplemented with soluble cytokines and...
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5985665 |
Biochemical analysis of antioxidant function of lymphocytes in culture
The present invention provides a cell culture medium useful for a biochemical analysis of antioxidant function in human lymphocytes, said medium comprising, a buffered, serum-free solution...
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5958404 |
Treatment methods for disease using co-localized cells and Sertoli cells obtained from a cell line
A method of treating a disease is provided that results from a deficiency of a biological factor which comprises administering to a mammal Sertoli cells and cells that produce the biological...
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5945337 |
Method for culturing CD34.sup.+ cells in a serum-free medium
A serum-free medium which supports the proliferation and differentiation of CD34 + cells purified from normal bone marrow, peripheral blood of patients treated with cytokines, and umbilical cord...
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5914070 |
Gas concentration adjusting agent, gas concentration adjusting method, and gas concentration adjusting system
A gas concentration adjusting agent which allows a low oxygen concentration environment, such as an ischemic environment for tests of cultured cell damage caused by low oxygen or the like, to be...
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5912175 |
Process and media for the growth of human cornea and gingiva
Media and methods are disclosed for the in vitro formation of a histologically complete human epithelium. The media are serum-free, companion cell or feeder layer free and organotypic, matrix free...
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5910443 |
Medium for culturing human olfactory neurons
The present invention relates to a culture of human olfactory neurons. The neurons may display a normal neuronal pathology or a pathology characteristic of a generalized central nervous system...
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5908782 |
Chemically defined medium for human mesenchymal stem cells
A composition and method for maintaining the viability of human mesenchymal precursor cells in a serum-free environment which composition includes (1) a minimum essential medium; (2) serum albumin;...
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5897987 |
Use of arabinogalactan in cell cryopreservation media
Methods and compositions for cryopreserving somatic cells are provided. In one embodiment, a cell cryopreservation medium is provided which includes an effective amount of arabinogalactan to...
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5888816 |
Cell cultures of and cell culturing method for nontransformed pancreatic, thyroid, and parathyroid cells
The present invention provides a method for producing an expanded, enriched, non-transformed human cell culture of human pancreatic, thyroid or parathyroid endocrine cells and other types of cells...
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5885835 |
Kit for in vitro cell growth of eucaryotes using low molecular weight peptides containing L-glutamine
A method and kit are provided for growing eucaryotic cells using a hydrolyzate of a protein material containing peptides and free amino acids. The hydrolyzed protein material contains L-glutamine,...
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5874306 |
Culturing human pancreatic endocrine cells in medium containing extracellular matrix from human bladder carcinoma cells
Human pancreatic endocrine cells are proliferated without loss of hormone function in a culture medium containing extracellular matrix from bladder carcinoma cell lines in the substantial absence...
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