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7846726 |
Human fetal bladder-derived epithelial cells
This invention discloses a substantially pure population of human urinary bladder-derived epithelial cells and methods of isolating and culturing the urinary bladder-derived epithelial cells. In...
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7439064 |
Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
The invention relates to methods for culturing human embryonic stem cells by culturing the stem cells in an environment essentially free of mammalian fetal serum and in a stem cell culture medium...
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7285646 |
Ultrapure transferrin for pharmaceutical compositions
The present invention relates to an ultrapure human transferrin, and to methods for manufacturing the ultrapure transferrin. The transferrin may be holo-, apo- or at any desired degree of iron...
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6733746 |
Hematopoietic cell culture nutrient supplement
The present invention provides a serum-free supplement which supports the growth of hematopoietic cells in culture. Also provided are a medium comprising a basal medium supplemented with the...
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6617161 |
Serum-free cell growth medium
A chemically defined-serum free growth medium for the in vitro and ex vivo of cells and cell lines. The medium consists of about a one to one ratio (v/v) of two basal growth media containing...
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6617159 |
Serum free medium for chondrocyte cells
Serum free media for growth and proliferation of chondrocytes and mesenchymal stem cells in culture are provided. A serum free medium for growth of chondrocytes includes a serum free composition...
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6413772 |
Cell structure media for mammalian cells
A chemically defined mammalian cell culture medium is provided that supports maintenance and long term clonal growth of mammalian hepatocytes and other cells.
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6372493 |
Hormone-secreting cells maintained in long-term culture
Methods are provided for the establishment and maintenance in long term culture of hormone secreting cells. Cells are derived from tumorous or non-tumorous animal or human tissues, including ovary,...
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6017760 |
Isolation and culture of porcine hepatocytes
A perfusion device such as a liver assist device containing a housing defining a perfusion inlet and a perfusion outlet, a porous membrane structure mounted within said housing to define a...
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5945337 |
Method for culturing CD34+ cells in a serum-free medium
A serum-free medium which supports the proliferation and differentiation of CD34+ cells purified from normal bone marrow, peripheral blood of patients treated with cytokines, and umbilical cord...
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5908782 |
Chemically defined medium for human mesenchymal stem cells
A composition and method for maintaining the viability of human mesenchymal precursor cells in a serum-free environment which composition includes (1) a minimum essential medium; (2) serum albumin;...
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5858783 |
Production of normal mammalian organ culture using a medium containing mem-alpha, leibovitz L-15, glucose galactose fructose
Normal mammalian tissue and the culturing process has been developed for the three groups of organ, structural and blood tissue. The cells are grown in vitro under micro-gravity culture conditions...
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5849585 |
Isolating and culturing Schwann cells
A method for enhancing the survival and/or proliferation of Schwann cells (especially human Schwann cells) in cell culture is disclosed which involves culturing the cells in serum free culture...
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5576194 |
Recombinant protein production
Recombinant Factor VIII production is increased by supplementing the serum-free culture of mammalian host cells containing the gene therefor in a serum-free nutrient media with lipoprotein obtained...
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5447861 |
Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro
Continuous mammalian cell lines having characteristics of immature monocytes and/or macrophages which have been established from normal mammalian cells, continuous human cell lines having...
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5409827 |
Blended bovine sera cellular growth media and their methods of production and use
The present invention provides media and methods for the maintenance or promotion of growth of a cellular line, particularly a hybridoma cellular line. A serum blend is disclosed comprising fetal...
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5342777 |
Cell culture medium for human liver epithelial cell line
The present invention relates to cell medium developed to support long term multiplication and permanent establishment of a cell line of human liver epithelial cells. The medium may contain an...
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5155036 |
Serum-free medium containing retinoic acid useful for cultivating human/human hybridomas
A serum-free medium for cultivating a human monoclonal antibody-producing human/human hybridoma, said medium comprising a serum-free complete medium and at least 10-12 M but not more than 10-6 M of...
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5063157 |
Serum-free culture medium for mammalian cells
The present invention provides a serum-free culture medium for mammalian cells, especially non-adherent cells, containing a base medium, transferrin, insulin and selenite, wherein it contains or...
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4927762 |
Cell culture medium with antioxidant
Cells grown in chemically defined (serum free) media are protected against endogenous oxidizing agents by a nontoxic antioxidant with free thiol groups, at cell densities less than about 105...
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4757005 |
Method and cell line for obtaining plasminogen activators
A method of increasing the yield of plasminogen activator from a plasminogen activator-producing cell line by adapting said cell line to grow in a suitable growth medium essentially free of fetal...
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4743552 |
Method for growth in tissue culture of normal colonic epithelial cells and method for determination of preneoplastic color cells
The invention concerns a method for the routine primary culture of human colonic epithelial cells within a monolayer. 12-0-tetradecanoylphorbol-13-acetate (TPA) enhanced DNA synthesis an average of...
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4654304 |
Composition for cell cultivation, production and use thereof
The present inventiona is directed to a mammalian serum-originated growth factor-containing composition useful in cell cultivation. The composition of the present invention is substantially free of...
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4615977 |
Method for the cultivation of normal diploid cells and cultivation medium used therefor
A method and medium for the multiplication of normal diploid cells from mammals is described, comprising cultivating the cells in a culture medium containing an amine derivative or derivatives...
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4608341 |
Living, fast-growing thyroid cell strain, FRTL-5
A fast growing, continuous, functional rat thyroid cell strain, FRTL-5, which maintains functional characteristics of iodide uptake and thyroglobulin synthesis over prolonged periods of culture is...
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4560655 |
Serum-free cell culture medium and process for making same
A defined serum-free medium that is capable of growing a wide range of suspension and monolayer cells includes a serum substitute composed of fetuin, transferrin, phosphatidylcholine, e.g.,...
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4443546 |
Process and composition for propagating mammalian cells
A serum-free medium for use in propagating cells.A process involving growing normal mammalian cells in vitro in a serum-free medium. The process includes providing the cells to be grown with...
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4440860 |
Stimulating cell growth
Compositions and methods for promoting cell growth featuring, in one aspect, cell culture media containing milk or colostrum and fibronectin; fibronectin is preferably pre-coated onto the culture...
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