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7547546 |
Progenitor cells from wharton's jelly of human umbilical cord
Human progenitor cells are extracted from perivascular tissue of human umbilical cord. The progenitor cell population proliferates rapidly, and harbours osteogenic progenitor cells and MHC−/−...
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7354733 |
Method for sorting and separating living cells
We disclose methods of sorting or separating mixtures of living cells (e.g., eukaryotic, prokaryotic, mammalian, pathogenic, bacterial, viral, etc.). We perform our methods by activating...
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7166477 |
Muscle sample prepared for prion assay
A method of preparing a sample of muscle tissue and of assaying the prepared sample to determine the presence of prions in the sample is disclosed. The muscle tissue is homogenized and mixed with a...
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7109028 |
Methods of isolating hepatic progenitors
This invention relates to methods of isolating hepatoblasts utilizing panning techniques and fluorescence activated cell sorting. This invention further relates to isolated hepatoblasts and to a...
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7049141 |
Use of collagenase in the preparation of neural stem cell cultures
The invention provides a method for using collagenase to dissociate neural stem cells in neural stem cell cultures. The collagenase treatment results in an increased cell viability and an increased...
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7045349 |
Method of islet isolation using process control
Advanced Islet Separation Technology incorporates an automated method, automated control methodology, process control interface, and automated apparatus to separate (isolate) and process pancreatic...
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6900051 |
Process for obtaining mammalian insulin secreting cells in vitro and their uses
The present invention relates to a process for obtaining mammalian insulin secreting cells in vitro, characterized in that it contains the following steps: a) preparation of the mammalian...
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6869771 |
Method for distributing equal volumes of attached confluent living cells
A method for distributing an equal volume of attached confluent living cells in each well of a multi-well plate used in a bioassay, comprising the following steps: (a) growing attached living...
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6833270 |
Apparatus and method for isolating cells from organs
An apparatus and method for extracting cells from organs in which a digestion chamber includes at least one inlet and at least one outlet, and a separator for retaining the organ and permitting the...
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6825027 |
Method of production of purified hepatitis a virus particles and vaccine preparation
The present invention provides methods of purification of Hepatitis A Virus from the supernatant of an infected cell culture and production of a preparation of purified HAV antigen. The present...
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6585969 |
Non-viable keratinocyte cell composition or lysate for promoting wound healing
Cultures of keratinocyte cells are provided which are free from nonautologous fibroblasts and organ extracts, and which have a high speed of cell amplification for a minimum seeding density. The...
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6489164 |
Isolation of cells from organ tissue using sonication
A method for isolating specific viable cell types from surrounding organ tissue is provided. The method entails the use of sonication in conjunction with tissue dissociating agents to free the...
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6383805 |
Epithelial cell cultures for in vitro testing
A method and a medium for culturing epithelial cells of both normal and malignant origin is provided. The method entails physically disaggregating tissue samples, placing the resulting fragments...
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6365405 |
Compositions of chondrocytes, preparation and utilization
This invention concerns compositions of chondrocytic cells, notably human ones, and the methods for preparing and using them. More specifically, the invention describes the production of autologous...
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6242252 |
Hepatic progenitors and method of isolating same
This invention relates to methods of isolating hepatic progenitors utilizing panning techniques and fluorescence activated cell sorting. This invention further relates to isolated hepatic...
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6238922 |
Use of collagenase in the preparation of neural stem cell cultures
The invention provides a method for using collagenase to dissociate neural stem cells in neural stem cell cultures when passaging aggregated neural stem cells. The collagenase treatment results in...
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6162643 |
Hepes based basal nutrient medium for the isolation and culturing of stem cells
Novel media and methods are disclosed for the growth of stem cells. The media are serum-free, companion cell or feeder layer free and organotypic, matrix free solutions for the isolation and...
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6107543 |
Culture of totipotent embryonic inner cells mass cells and production of bovine animals
The use of totipotent embryonic stem cells to provide substantially identical cells for embryo cloning techniques is described. The method includes the culture of loose suspensions of inner cell...
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5994619 |
Production of chimeric bovine or porcine animals using cultured inner cell mass cells
Novel cultured inner cell mass (CICM) cells, and cell lines, derived from ungulates, in particular, pigs and cows, and methods for their preparation are provided. The subject CICMs possess similar...
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5925531 |
Human bone marrow endothelial cell line and methods of use thereof
The present invention provides immortalized human bone marrow endothelial cells which are useful for the study of tumor metastasis. In particular, the human bone marrow endothelial cell lines...
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5905042 |
Cultured inner cell mass cell lines derived from bovine or porcine embryos
Novel cultured inner cell mass (CICM) cells, and cell lines, derived from ungulates, in particular, pigs and cows, and methods for their preparation are provided. The subject CICMs possess similar...
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5888705 |
Compositions and method of stimulating the proliferation and differentiation of human fetal and adult pancreatic cells ex vivo
A method of inducing the proliferation and/or differentiation of human adult pancreatic cells entails contacting primary cultures of such cells with Hepatocyte Growth Factor/Scatter Factor...
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5866167 |
Non-viable total keratinocyte lysate for promoting wound healing
Cultures of keratinocyte cells are provided which are free from nonautologous fibroblasts and organ extracts, and which have a high speed of cell amplification for a minimum seeding density....
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5855620 |
Matrix substrate for a viable body tissue-derived prosthesis and method for making the same
Disclosed is a process for treating body tissues such as vascular structures to remove barriers to recellularization before and after implantation in a human host. The body tissue, for example a...
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5851833 |
Neomorphogenesis of urological structures in vivo from cell culture
Methods and artificial matrices for the growth and implantation of urological structures and surfaces are disclosed in which urothelial cells are grown in culture on biodegradable, biocompatible,...
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5827735 |
Pluripotent mesenchymal stem cells and methods of use thereof
A scar inhibitory factor protein isolate from mammalian basement membranes is provided that inhibits lineage commitment and differentiation of stem cells in vitro and in vivo. The protein isolate...
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5814511 |
Human breast epithelial cell type with stem cell and luminal epithelial cell characteristics
Described is a substantially purified human breast epithelial cell (Type I HBEC) displaying the following characteristics: variable cell shape; smooth cell colony boundary; deficiency in gap...
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5622857 |
High performance cell culture bioreactor and method
A high performance hollow fiber bioreactor having concentric hollow fiber bundles: a central hollow fiber bundle supplies media, and an outer array supplies oxygen needed for cell culture. Useful...
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5100799 |
Method for releasing cell cultures from microcarriers
This invention relates to a method for releasing cell cultures from microcarriers in which a trypsin solution is directed through a container with the microcarriers therein in a flow-through...
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5081030 |
Release of cells from affinity matrices
The present invention comprises a process for release from the cell-receptor complex of positively selected cells in viable, functional condition, where a ligand involved in the particular...
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4556639 |
Method and apparatus for dislodging cultured cells
A method for dislodging cultured cells has the steps of preparing a culture container which has a growing surface on which cultured cells are grown, and which is filled with a culture solution in...
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4339537 |
Method of culturing biological substances
Biological substances such as tissues, cells, vaccines and viruses are cultured by the steps of: introducing a culture solution having the substances suspended therein into a closed culture vessel...
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4304866 |
Transplantable sheets of living keratinous tissue
A method of producing transplantable sheets of living keratinous tissue by culturing keratinocytes in a culture vessel and subsequently enzymatically detaching a sheet of keratinous tissue...
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4154652 |
Method for automatically and successively cultivating tissues or cells of a body
Tissues or cells of a living body, especially a human body, are automatically and successively cultivated in a nutrient solution filled culture container disposed in a gas-sealed culture box by...
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4059485 |
Adaptation of cell lines to suspension culture
Densely packed cells growing rapidly in substrate-attached culture are gently released from the substrate by enzymatic treatment to form aggregates ranging from 20 μ to 500 μ in diameter. These...
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3450598 |
METHOD FOR CELL PROPAGATION
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