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7423135 β-actin and rpS21 promoters and uses thereof  
The invention relates to isolation of novel β-actin and ribosomal protein S21 (rpS21) promoters and uses thereof. In particular, this invention features nucleotide sequences for rodent β-actin p...
7280680 Method and apparatus for observing three-dimensional localizations of in vivo expressed genes as well as method and apparatus for observing minute three-dimensional localizations of in vivo expressed genes  
In order to make observation of localizations of in vivo expressed genes possible in an entire individual living organism such as animals, and plants, a living genetic recombinant specimen...
RE39792 Method for culturing Chinese hamster ovary cells  
A biochemically defined culture medium for culturing engineered Chinese hamster ovary (CHO) cell lines, which is essentially free from protein, lipid and carbohydrate isolated from an animal...
7166445 Methods and host cells for improved cell culture  
The invention provides improved methods of recombinant protein production in cell culture. More specifically, the invention relates to the modulation of the IGF-1 signaling pathway in cells so as...
7091004 Regulation of translation for recombinant protein production  
The invention provides cells that produce increased levels of recombinant protein by modulating the activity of translational regulator gene products that are downstream targets of PKB alpha,...
6667172 Cell and tissue culture modeling device and apparatus and method of using same  
A cell and tissue culture modeling device comprising a housing having an interior chamber, an inlet port in fluid communication with the internal chamber, an outlet port in fluid communication with...
5985607 Recombinant DNA molecules and expression vectors for tissue plasminogen activator  
A recombinant DNA molecule adapted for transfection of a host cell comprising a nucleic acid molecule encoding mammalian erythropoietin or tissue plasminogen activator, an expression control...
5763253 Methods of preparing tissue plasiminogen activator derivative composition  
Tissue plasminogen activator (t-PA) derivatives are produced in useful quantities using recombinant DNA techniques. Specific derivatives include amino acid deletion derivatives and amino acid...
5753486 Human tissue plasminogen activator  
A human tissue plasminogen activator (t-PA) is produced in useful quantities using recombinant DNA techniques. The invention disclosed thus enables the production of t-PA free of contaminants with...
5736135 Method for making variant secreted proteins with altered properties  
A screening method for the selection of mutagenized proteins that are normally secreted by cells is described. The method includes the development of a cloning vector for the expression of...
5728565 Methods of preparing tissue plasminogen activator derivatives  
Tissue plasminogen activator (t-PA) derivatives are produced in useful quantities using recombinant DNA techniques. Specific derivatives include amino acid deletion derivatives and amino acid...
5658759 Recombinant DNA expression vectors  
The invention provides expression vectors containing the promoter, enhancer and substantially complete 5'-untranslated region including the first intron of the major immediate early gene of human...
5658788 Compounds and methods for treatment of thromboembolic disorders  
The present invention provides derivatives of tissue plasminogen activator that lack the Finger, Growth Factor and Kringle 1 domains and comprise a Kringle 2 domain that is monoglycosylated at a...
5646009 Hybrid vector and method resulting in protein overproduction by eukaryotic cells  
A hybrid vector carrying a first and second DNA segments operationally linked thereto, the first DNA segment encoding a protein capable of cross-linking to the cap structure of mRNA and mediating...
5641655 Methods for producing thrombopoietin polypeptides using a mammalian tissue plasminogen activator secretory peptide  
DNA constructs useful in the production of thrombopoietin are disclosed. In general, the DNA constructs comprise a first DNA segment encoding a fusion of an amino-terminal secretory peptide joined...
5510248 Stable recombinant meizothrombin-like polypeptides  
An isolated polynucleotide which encodes human prothrombin is modified to encode amino acid substitutions at positions 155, 271 and 284 of the prothrombin polypeptide. A second polynucleotide is...
5439679 Peptide plasminogen activators  
Novel polypeptide compositions based on the amino acid sequence of tissue plasminogen activator (tPA) are provided having improved properties over natural tissue plasminogen activator....
5407819 Human plasminogen activator variants having amino acids 37-42 substituted and a method for their manufacture  
A TPA variant with replacement of amino acids is provided. In a typical embodiment, the variant has an amino acid sequence with replacement of the amino acid group consisting of asparagine at the...
5346824 DNA encoding variants of tissue plasminogen activators and expression vectors and hosts thereof  
A fibrinolytically active amino acid sequence variant of a plasminogen activator is prepared that has one or more glycosylation sites in regions that are not glycosylated in the native molecule....
5342775 Plasminogen activator  
A novel plasminogen activator which is identical in peptide sequence to naturally occurring human prourokinase except that the 155th amino acid counting from the N-terminal amino acid (serine)] is...
5270198 DNA molecules encoding variants of tissue plasminogen activators, vectors, and host cells  
A fibrinolytically active amino acid sequence variant of a plasminogen activator is prepared that has one or more glycosylation sites in regions that are not glycosylated in the native molecule....
5246850 DNA molecules encoding human tissue plasminogen activator variants with decreased clearance, vectors, and host cells  
Biologically active tissue plasminogen activator (t-PA) variants with decreased clearance as compared to wild-type t-PA are prepared, including variants that have one or more amino acid alterations...
5244806 DNA encoding novel tissue plasminogen activator derivatives having kringles 1 and 2 deleted, vectors and host cells  
The invention discloses modified forms of the enzyme tissue plasminogen activator (t-PA) in which recombinant DNA techniques are utilized to modify the structure of the t-PA cDNA to express a...
5242819 DNA molecules encoding hybrid proteins of tissue plasminogen activator and urokinase  
Novel single-chain hybrid plasminogen activators having an amino acid sequence composed of at least two subsequences corresponding in amino acid identity and number to subsequences of human t-PA...
5219738 Animal cell transformant, method for obtaining the transformant and method for producing desired foreign gene product by using the transformant  
By screening an animal cell transformant, which has been obtained by introducing into wild-type animal cells a wild-type DHFR gene and a structural gene which codes a desired useful substance, with...
5147643 DNA sequences encoding human t-PA substituted at position 275 or at positions 275 and 277 and pharmaceutical compositions  
Biologically active mutant tissue plasminogen activators are disclosed wherein site directed mutagenesis, for example, of a two-chain activation site renders the mutants resistant to conversion to...
5122458 Use of a bGH gDNA polyadenylation signal in expression of non-bGH polypeptides in higher eukaryotic cells  
The present invention relates to the use of the polyadenylation signal from the gene for bovine growth hormone to achieve a high level of expression of peptides in eukaryotic cells. Accordingly,...
5106741 Tissue plasminogen activator (TPA) analogs  
This invention discloses human tissue plasminogen activator (TPA) analogs. The analogs are TPA-like molecules that have had the native domain regions either rearranged, delected, added or a...
5087572 DNA encoding human plasminogen modified at the cleavage site  
A cleavage-resistant plasminogen molecule is provided that is conveniently produced in recombinant cells by expression of a nucleic acid sequence encoding the plasminogen molecule. Preferably the...
Matches 1 - 29 out of 29