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5286627 Method of high-sensitive analysis of bile acid and reagent composition for the analysis  
A method for a high-sensitivity quantitative analysis of bile acid in a bile acid-containing sample utilizes a reagent comprising: (1) a steroid dehydrogenase which is capable of effecting a...
5283181 Conjugated enzyme electrode  
A conjugated enzyme electrode comprising diaphorase and an amino-acid dehydrogenase immobilized on a conductive support by a polyfunctional aldehyde is disclosed, by which amino acids can be...
5279945 Method for the enzymatic determination of aspartame  
The aspartame content in aqueous solutions is determined by enzymatic cleavage of aspartame to aspartic acid and phenylalanine methyl ester by means of a peptidase, followed by a detection...
5278044 Stable aqueous NADH reagent and kit  
An aqueous coenzyme reagent composition contains NADH, a carbonate/bicarbonate buffer (with a pH of about 9.5-11) and water. Both NADH and the buffer are at low concentrations, e.g., about 2-5 mM...
5278047 Method of analysis, reagent composition and use thereof for glucose determination  
A method, a reagent composition and the use thereof for quantitative determination of total glucose in undiluted whole blood are described. A sample of undiluted whole blood is contacted with a...
5272062 Measurement of catalase activity  
A method of measurement of catalase activity in a sample suspected of containing such activity which comprises treating said sample with a peroxide, an alcohol, nicotinamide adenine dinucleotide...
5272054 Assay by enzyme-catalyzed isotopic exchange  
A method of assay for isotopically exchangeable analytes is disclosed. Analytes are labeled by enzymatic exchange of a hydrogen atom of the analyte and a deuterium or tritium atom. Preferably,...
5270174 Method and kit for indicating the level of oral microbial activity  
The invention provides a method for indicating the level of oral microbial activity comprising providing a sterile liquid for introduction into the oral cavity for swishing therein, providing a...
5266463 Highly sensitive assay method for L-carnitine and composition for practicing same  
A method of assaying L-carnitine in a specimen comprises reacting a specimen containing L-carnitine with: a) L-carnitine dehydrogenase having coenzymes of the thio-NAD group and of the NAD group,...
5266462 Measurement of platelet activities  
Methods are provided for measuring the procoagulant activity of platelets in blood by means of a chromogenic assay which is specific for procoagulant phospholipids. These methods include the...
5264347 Method for the determination of α-ketoisocaproate in biological samples using D-α-hydroxyisocaproic dehydrogenase from lactobacillus casei  
The selective determination of α-ketoisocaproate in samples of body fluids, especially in serum, is achieved, without previous separation, directly in the fluid and in the presence of NADH, by...
5262305 Interferant eliminating biosensors  
A biosensor including an interferant-eliminating catalyst and method for analyzing an analyte in a biological sample is disclosed.
5260197 L-fucose dehydrogenase, a process for production thereof, quantitative assay for L-fucose using said enzyme and a kit for quantitative assay  
L-Fucose dehydrogenase having the following Physicochemical properties (1) and (2): (1) Action and substrate specificity It withdraws hydrogen from L-fucose and converts into L-fuconolactone and...
5258286 Method of terminating isocitrate dehydrogenase reaction  
Urea, creatinine, creatine, triglycerides, or the like in a specimen can be accurately determined by terminating an isocitrate dehydrogenase reaction by addition of a chelating agent in a system...
5250415 Process for the preparation of glucose dehydrogenase from Bacillus megaterium  
A process for the preparation of glucose dehydrogenase from Bacillus megaterium. This invention relates to the preparation of a genetically engineered polypeptide with the biological activity of...
5250420 Method and reagent for determination of dehydrogenase or its substrate  
A method for determining dehydrogenase or its substrate in dehydrogenase reaction, which comprises subjecting a dehydrogenase and its substrate to dehydrogenase reaction in the presence of an...
5227307 Test for the rapid evaluation of ischemic state  
A method for detecting ischemic states in a patient by contacting a sample of serum, plasma, fluid or tissue with a metal ion capable of binding to metal ion binding sites in the sample to form a...
5223390 Analytical elements containing non-reactive blocking dyes  
An analytical element for quantitatively assaying an analyte by measuring reflection density in the range 320 to 360 nm. The element comprises a reagent layer and a registration layer. One of such...
5213966 Quantitative determination of glucose utilizing enzyme cascade system  
A procedure is disclosed for the determination of glucose in a biological liquid, as well as a reagent mixture for use in conjunction with the procedure. The biological liquids concerned are...
5206147 Colorimetric assay by enzymatic oxidation in the presence of an aromatic nitroso or oxime compound  
The present invention provides a process for the colorimetric determination of an analyte by means of enzymatic oxidation of the analyte in the presence of an electron acceptor and determination...
5200325 Self-indicating analysis employing stoichiometric chemical subtraction  
An analytical method and test composition for determining whether an analyte is present in a test sample at a predetermined concentration level by measuring or observing a preselected level of...
5198336 Bioassay for chemicals which generate prooxidant states  
A bioassay making use of submitochondrial particles to test for the presence of toxic substances which induce prooxidant states in vivo. The assay uses complex I of the electron transport enzymes...
5198335 Integral multilayer analytical element for analysis of ammonia-forming substrate  
An integral multilayer analytical element for the analysis of an ammonia-forming substrate comprising: (I) a light-transmissive, liquid-impermeable support: (II) an ammonia indicator layer...
5196314 Process and reagent for the determination of substrates or enzyme activities  
A process for the determination of substrate or enzyme activities by the use of a redox reaction as a measurement reaction is carried out in the presence of one or more additionally added...
5196340 Enzyme electrode containing an enzyme and a coenzyme immobilized in separate layers of a membrane  
An enzyme electrode comprising an enzyme and a coenzyme immobilized in at least one layer is provided. This electrode has an outer membrane preventing the coenzyme from dissipation and so...
5190863 Composition for determining the presence or concentration of D-β-hydroxybutyrate  
A composition, test device and method of determining the presence or concentration of ketone bodies, and specifically D-β-hydroxybutyrate, in a test sample are disclosed. The test device includes...
5188941 Enzymatic determination of theophylline  
The present invention provides a new methodology and test composition for determining the presence of theophylline in test samples. The methodology employs enzymes that utilize or recognize...
5185249 Dry analytical element for assaying salicylate  
A dry multilayer element for assaying salicylate is disclosed.
5166049 Measurement of diaphorase activity and reagent therefor  
A method for measuring diaphorase activity comprising mixing a sample comprising diaphorase with nitro blue tetrazolium, EDTA or a salt thereof, at least one of nicotinamide adenine dinucleotide...
5162516 Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis  
The alcohol dehydrogenase II gene from Zymomonas mobilis has been cloned and sequenced. This gene can be expressed at high levels in other organisms to produce acetaldehyde or to convert...
5162203 Methods of measuring isozymes and isozyme classes of alcohol dehydrogenase  
The present invention provides fluorescence-based methods for sensitively detecting total ADH activity in human sera and selectively measuring the activity of different classes of ADH in human...
5158873 Method and reagent for determining LD-1 isoenzyme  
A rapid and specific method and reagent for determining LD-1 isoenzyme in biological fluids by incorporating a chaotropic agent, such as sodium perchlorate, into an LDH assay reagent system. The...
5156955 Nucleoside oxidase and assay method utilizing same  
The present invention provides a novel nucleoside oxidase YT-1 serving as an enzyme for catalyzing oxidation reaction of a nucleoside, and oxidase being characterized in that the oxidase causes...
5141854 Enzymatic composition for ethanol assay  
An enzymatic assay composition for ethanol determination is provided. A compound of the formula H2 N--(CH2)n --CHR--CH2 --NH2where R is H, CH3, or OH and N is 0-4acts as a buffer and a trapping...
5141853 Determination of ammonia levels in a sample  
A method and kit for determining the amount of ammonia in a body fluid. The method and kit involve contacting the fluid with glutamate dehydrogenase, alpha-ketoglutarate and nicotinamide...
5141855 Signal amplifying cobalt (III) redox reagents and methods for the determination of analytes in aqueous fluids  
Reagents and methods for the determination of analytes are disclosed, using water soluble cobalt(III) complexes and metallizable dyes to form cobalt(III) complexes of the metallizable dyes....
5126275 Analytical method using tetrazolium salt indicators having a reflectance plateau  
General classes of 2-thiazolyl tetrazolium salt compounds have been found to be characterized by a reflectance spectrum exhibiting an extended plateau above about 600-650 nm. Such compounds are...
5126245 Reagents for assay of gamma-glutamyltranspeptidase  
A reagent for assaying γ-glutamyltranspeptidase (γ-GTP) which contains an amino acid dehydrogenase and a substrate specific to γ-glutamyltranspeptidase activity. The reagent can be used to assay...
5126247 Method, system and devices for the assay and detection of biochemical molecules  
Oxygen-independent methods, systems and devices for the enzymatic colorimetric assay and detection of biochemical analytes. Two systems are described, both of which produce less than one...
5124141 Method for diagnosing malaria  
A diagnostic method for the detection of human malaria infections. Specifically, blood samples are combined with a reagent containing 3-acetyl pyridine adenine dinucleotide (APAD), a substrate...
5122454 Assay method for lecithin-cholesterol acyltransferase  
An assay with high sensitivity for activity of lecithin-cholesterol acyltransferase in blood for functional analysis of liver, by bringing the blood into contact with lecithin and free cholesterol...
5122456 Amperometric method for the quantitative determination of 1,4-dihydronicotinamide adenine dinucleotide (NADH) in solution  
A method is disclosed for the quantitative determination of 1,4-dihydronicotinamide adenisne dinucleotide (NADH) in solution. The method comprises contacting the NADH-containing solution with an...
5116732 Tetrazolium halide compounds and methods  
Compounds of the formula DNB-TZ(P2) (P3) X, wherein TZ is a tetrazolium ring, DNB is 5-(2,4-dinitrophenyl), X is halide and P2 and P3 are each independently halophenyl, nitrophenyl or phenyl. The...
5116759 Reservoir chemical sensors  
Single or multi-cell reservoir sensors with single illumination sources and one or more detectors per cell unit have an arrangement whereby a gaseous, vapor or liquid sample enters the cell body...
5116728 Reagents for CO2 detection  
A stabilizer for maintaining a constant level of CO2 in coenzyme containing enzymatic CO2 diagnostic reagents is disclosed, comprising rate-limiting amounts of (a) the diagnostic reagents and (b)...
5112740 Carbon dioxide assay for body fluids comprising carbonic anhydrase  
A multilayer element for assaying carbon dioxide is disclosed. The elememt comprises a spreading layer comprising acetazolamide, a reagent layer comprising phosphoenolpyruvate carboxylase and...
5112741 Acetaldehyde trapping system  
The invention provides a reagent system for the enzymatic determination of an oxidizable substrate in a fluid sample; the system includes an active amine trap for inactivating high concentrations...
5108905 Method of assaying magnesium in human body fluid  
Disclosed is a method of assaying the content of Mg ions in a human body fluid such as serum, urine or saliva by the use of a reactant solution containing isocitrate dehydrogenase, NADP+,...
5108903 Reducible compounds which provide leuco dyes for analytical compositions, elements and methods of using same  
Certain reducible compounds are useful in analytical compositions, elements and methods, for example for the detection of bacterial cells. These compounds comprise a moiety which provides a leuco...
5094943 Process and reagent for the improved quantitative colorimetic determination of hydrogen peroxide  
The present invention provides a process for the improved colorimetric determination of hydrogen peroxide as formed by a hydrogen peroxide-producing oxidase, by addition of a chromogenic system...