Match Document Document Title
5492815 Method of determining glucose-6-phosphate and composition therefor  
A method is provided for determining glucose-6-phosphate, including the step of dehydrogenating glucose-6-phosphate and NAD or NADP in the presence of glucose-6-phosphate dehydrogenase to produce...
5486459 Biologically relevant methods for the rapid determination of sterility  
The invention is directed to biological processes and apparatus for determining the efficacy of a sterilization cycle based upon the recovery of activity of interactive enzyme systems comprising...
5484708 Method for the colorimetric determination of an analyte with a PQQ-dependent dehydrogenase  
The invention concerns a method for the colorimetric determination of an analyte by means of enzymatic oxidation with a PQQ-dependent dehydrogenase in the presence of an electron acceptor from the...
5480777 Kinetic enzyme assay for determining the CO2 content of body fluids using PEP carboxylase with inhibitor  
An improved kinetic assay is provided for spectrophotometrically determining the dissolved carbon dioxide (CO2) content of a body fluid (e.g., blood, plasma or serum), wherein at about pH 8.0 the...
5474907 Multilayer analytical element for salicylate assay  
A multilayer analytical element for measuring salicylate is disclosed. The element has a support. The support is coated with the following layers, top down to the support:a) a spreading layer,b) a...
5462858 Dry multilayer analytical elements for assaying transaminases  
A dry multilayer analytical element for assaying transaminases wherein the analytical element comprises: a) a transaminase substrate; b) reagents that generate pyruvate in the presence of a...
5460948 Assay of salicylates or reduced pyridine nucleotides  
A method for the estimation of a salicylate in a sample is characterized by enzymatically converting the salicylate to a catechol by the action of a salicylate mono-oxygenase enzyme on the...
5459032 Method of conducting a non-instrumental test to determine catalyst presence  
A method and apparatus for performing an enzyme spot test without traditional instrumentation is disclosed. The method utilizes various reagents to allow the visual determination of the presence...
5453360 Oxidative coupling dye for spectrophotometric quantitive analysis of analytes  
A dye couple, comprising 3-methyl-2-benzothiazolinone hydrazone (MBTH) and 8-anilino-l-naphthalenesulfonate (ANS), is used as an indicator in a reaction cascade producing a strong oxidizing agent,...
5449609 Methods for screening for neurotoxicity using a clonal human teratocarcinoma cell line  
Methods for screening for excitotoxic effects of an agent on neurons of the central nervous system are provided by the present invention.
5447847 Quantitative determination of pyruvic acid and quantitative analysis for component of living body making use of such determination  
Disclosed herein is a quantitative determination of pyruvic acid, which comprises reacting oxidized nicotinamide adenine dinucleotide, a pyruvate dehydrogenase complex and coenzyme A to a specimen...
5445946 Intravacuolar stains for yeast and other fungi  
The invention relates to a method of forming distinctive intravacuolar structures that are unique to fungal cells and can be correlated with cell viability. The preferred dyes of the present...
5445943 Method for the colorimetric determination of an analyte by means of benzyl alcohol dehydrogenase and a chromogenic redox indicator  
The invention concerns a method for the colorimetric determination of NADH by means of enzymatic oxidation with benzyl alcohol dehydrogenase whereby an aromatic nitrosoaniline compound of the...
5436133 Enzyme assay of biochemical substances  
This invention relates to an enzyme assay for quantitative analysis of biochemical substances using NAD(P)-NAD(P)H system and utilizing hydrogen peroxide, in which isocitric acid, metal ions and...
5434082 Early diagnosis of mastitis or garget  
Early diagnosis of mastitis or garget is carried out by measuring the concentration of 3-hydroxybutyric acid in milk. Used to this end is a reagent reacting with 3-hydroxybutyric acid to show a...
5434055 Compositions useful in anaerobic determination of analytes  
The invention describes a composition useful in determining sample analytes, where the determination is carried out anaerobically. The compositions include an analyte oxidizing agent, an electron...
5429931 Multilayer analytical element containing crosslinked binder and method for the determination of ethanol  
A multilayer analytical element has been prepared for accurate and rapid colorimetric determination of ethanol in aqueous specimens using alcohol dehydrogenase and an oxidized nicotinamide...
5429932 Multilayer analytical element containing niacinamide and method for the determination of ethanol  
A multilayer analytical element has been prepared for accurate and rapid colorimetric determination of ethanol in aqueous specimens using alcohol dehydogenase and an oxidized nicotinamide...
5426033 Method for enzymatic determination of substrate after enzymatically removing galactose  
A method for quantitating accurately 1,5-anhydroglucitol, fructose or amylase in a sample that contains galactose using an enzymatic determination involves a pretreatment which removes galactose...
5424204 Method for stabilizing glucose 6-phosphate dehydrogenase with hydroxylamines, aldehyde scavengers, dimethylthiocarbamoyl chloride or 2-(2-aminoethylamino) ethanol  
Glucose 6-phosphate dehydrogenase is stabilized in a clinical assay reagent with at least one stabilizer selected from among hydroxylamines, aldehyde scavengers dimethylthiocarbamoyl chloride,...
5420008 Assay method and assay reagent for serum iron or unsaturated iron binding capacity  
The present invention measures serum iron by liberating iron from transferrin under acid conditions, and contacting a test sample with a reaction system which utilizes at least aconitase, a...
5416004 Multilayer analytical element containing primary amine buffer and method for the determination of ethanol  
A multilayer analytical element has been prepared for accurate and rapid colorimetric determination of ethanol in aqueous specimens using alcohol dehydogenase and an oxidized nicotinamide...
5413916 Colorimetric toxicity test  
A method is described for determining the toxicity of an environmental sample to bacteria. The method includes the use of resazurin dye as an indicator. Glutaraldehyde is used as an accelerating...
5411866 Method and system for determining bioactive substances  
An apparatus and method for measuring glutamine and glucose in certain cell cultures and body fluids in the presence of interfering endogenous compounds e.g. ascorbic acid, uric acid or glutamic...
5407818 Biosensor containing a biochemical substance immobilized on a layer of olefinic-unsaturated, epoxy functional cross-linked polysiloxane  
A biosensor is prepared having a selective detection system containing a biochemical substance such as an enzyme immobilized on an olefinic-unsaturated, epoxyfunctional polysiloxane. Prior to...
5403450 Method of water purification  
A method for converting reducible or oxidizable substances from aqueous solution is claimed in which the aqueous solution to be treated is brought into contact with immobilized oxidoreductases, if...
5387109 Fructosylamine deglycase and a method of producing it  
Disclosed are a novel fructosylamine deglycase characterized by the specificity to amadori compounds of catalyzing oxidation of the compounds to produce an α-ketoaldehyde, an amine derivative and...
5385828 Method for determining the relative amounts of all cholesterol-containing lipoproteins in body fluids  
The invention refers to a method for determining the relative amounts of all cholesterol-containing lipoproteins in body fluids comprising electrophoretically separating the lipoproteins of an...
5382522 Immunoassay for creatine kinase-MB and creatine kinase-BB isoforms and reagents  
Methods and reagents for determining the lapse of time since an acute disease event, such as the occurrence of a myocardial infarction, are presented. A serum or plasma sample is assayed to...
5376534 Use of decreased 3-alpha-hydroxysteroid dehydrogenase activity in peripheral lymphocytes or other cells of patients with primary open angle glaucoma as a diagnostic indicator  
An assay for determining patients either having Primary Open Angle Glaucoma or at risk of developing Primary Open Angle Glaucoma. The assay involves testing cells, preferably lymphocyte cells, for...
5374536 Synergistic product selection test for biocides  
The product selection test is developed for rapid determination of the presence of synergistic blends of the biocide or the presence of biocide blends in contaminated waters. The method uses a...
5372934 Method for determining lactate dehydrogenase isoenzyme LD1 by stabilizing LD1 and inactivating isoenzymes LD2-LD5  
The invention is drawn to a diagnostic assay for lactate dehydrogenase isoenzyme LD1 in a sample containing lactate dehydrogenase isoenzymes LD1-LD5. The disclosed assay involves the following...
5364765 Method and reagent system for assaying isoenzyme profiles  
The present invention discloses a rapid assay method for quantitatively determining the complete isoenzyme profile of a biological fluid and a multiple-assay reagent system for carrying out the...
5362628 Fluorescent haloalkyl derivatives of reporter molecules well retained in cells  
The subject invention provides a method for analyzing the metabolic activity in cells by improving the retention of a detectable reporter molecule only in intact cells where a particular enzyme is...
5360718 Diagnosis of Candida vaginitis by detecting D-arabinitol in vaginal fluid  
A method for the presumptive detection of Candida in vaginal fluid, thereby providing an aid to the diagnosis of Candida vaginosis. A sample of vaginal fluid is combined with a test composition...
5358855 Inosinic acid dehydrogenase assay  
A cyto(histo)chemical technique for determining inosine 5'-monophosphate dehydrogenase (IMPD) presence and concentration within one or more morphologically classified or classifiable cells taken...
5356780 Determination of substrate of coenzyme requiring enzyme  
The invention relates to a competitive binding assay technique for substances (e.g. small molecules having no antigenic determinant) which can serve as substrates for coenzyme-requiring enzymes....
5356790 Highly sensitive assay method for myo-inositol, composition for practicing same, novel myo-inositol dehydrogenase, and process for producing same  
Myo-inositol in a specimen is assayed by reacting a specimen containing myo-inositol with: a) myo-inositol dehydrogenase using a thio-NADP group or thio-NAD group and an NADP group or NAD group as...
5356786 Interferant eliminating biosensor  
Interferant eliminating analyte sensors and sensing process prevent erroneous assays. Glucose electrodes were coated with an oxidizing enzyme (peroxidase) which allows hydrogen peroxide to...
5354652 Lead assay  
Lead assays using δ aminolevulinic acid dehydratase are disclosed.
5340722 Method for the determination of the concentration of an enzyme substrate and a sensor for carrying out the method  
A method for the continuous and reversible determination of the concentration of an enzyme substrate such as glucose in an specimen, wherein the specimen is brought into contact with a...
5334507 Composition for measurement of potassium ion concentration and composition for elimination of ammonium ions  
There is disclosed a composition for measurement of potassium ion concentration, which contains a) pyruvate kinase, b) glycerol kinase, c) glycerol-3-phosphate oxidase, d) peroxidase, e) a...
5326697 Composition and method of assaying for D-β-hydroxybutyrate  
A composition, test device and method of determining the presence or concentration of ketone bodies, and specifically D-β-hydroxybutyrate, in a test sample are disclosed. The test device includes...
5320954 Aromatic substituted glycoside  
An aromatic substituted glycoside is disclosed of the formula ##STR1## wherein the configuration of the substituted --OR on the anomeric carbon is alpha, n is an integer of 0 or 1, and R is a...
5304467 Device for assay of liquid sample  
A device for colorimetric assay of at least one component in a liquid sample, which device comprises a support, a reagent layer formed on a part of one surface of the support and a...
5302513 Method for determination of components  
The present invention relates to a method for quantitative determination of NAD(P)H derived from a specific component, which comprises converting NAD(P)H present in a sample or formed from...
5298414 Detection of morphine using morphine dehydrogenase  
Two enzymes, one an acetylmorphine carboxyesterase (AMCE), the other a morphine dehydrogenase (MDH), have been isolated from bacteria. The AMCE degrades heroin to morphine and the MDH oxidises...
5298144 Chemically wired fructose dehydrogenase electrodes  
An enzyme electrode is disclosed for use in voltammetric systems wherein a PQQ dehydrogenase enzyme is chemically bonded to a water soluble polyvinylpyridine osmium bipyridine polymer. The enzyme...
5290536 Phenyl substituted 2-thiazolyl tetrazolium salt indicators  
Phenyl substituted 2-thiazolyl tetrazolium salt compounds characterized by a reflectance spectrum exhibiting an extended plateau above about 600-650 nm. Such compounds are useful as chromogenic...
5290683 Rapid analysis of ethanol in body fluids  
An assay for directly and rapidly determining the alcohol concentration of a lower alcohol in a biological fluid without dilution, comprises a solid carrier. The carrier is impregnated with a...