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5260197 |
L-fucose dehydrogenase, a process for production thereof, quantitative assay for L-fucose using said enzyme and a kit for quantitative assay
L-Fucose dehydrogenase having the following Physicochemical properties (1) and (2): (1) Action and substrate specificity It withdraws hydrogen from L-fucose and converts into L-fuconolactone...
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5258286 |
Method of terminating isocitrate dehydrogenase reaction
Urea, creatinine, creatine, triglycerides, or the like in a specimen can be accurately determined by terminating an isocitrate dehydrogenase reaction by addition of a chelating agent in a system...
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5250420 |
Method and reagent for determination of dehydrogenase or its substrate
A method for determining dehydrogenase or its substrate in dehydrogenase reaction, which comprises subjecting a dehydrogenase and its substrate to dehydrogenase reaction in the presence of an...
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5250415 |
Process for the preparation of glucose dehydrogenase from Bacillus megaterium
A process for the preparation of glucose dehydrogenase from Bacillus megaterium. This invention relates to the preparation of a genetically engineered polypeptide with the biological activity of...
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5227307 |
Test for the rapid evaluation of ischemic state
A method for detecting ischemic states in a patient by contacting a sample of serum, plasma, fluid or tissue with a metal ion capable of binding to metal ion binding sites in the sample to form a...
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5223390 |
Analytical elements containing non-reactive blocking dyes
An analytical element for quantitatively assaying an analyte by measuring reflection density in the range 320 to 360 nm. The element comprises a reagent layer and a registration layer. One of such...
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5213966 |
Quantitative determination of glucose utilizing enzyme cascade system
A procedure is disclosed for the determination of glucose in a biological liquid, as well as a reagent mixture for use in conjunction with the procedure. The biological liquids concerned are...
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5206147 |
Colorimetric assay by enzymatic oxidation in the presence of an aromatic nitroso or oxime compound
The present invention provides a process for the colorimetric determination of an analyte by means of enzymatic oxidation of the analyte in the presence of an electron acceptor and determination of...
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5200325 |
Self-indicating analysis employing stoichiometric chemical subtraction
An analytical method and test composition for determining whether an analyte is present in a test sample at a predetermined concentration level by measuring or observing a preselected level of...
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5198335 |
Integral multilayer analytical element for analysis of ammonia-forming substrate
An integral multilayer analytical element for the analysis of an ammonia-forming substrate comprising: (I) a light-transmissive, liquid-impermeable support: (II) an ammonia indicator layer...
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5198336 |
Bioassay for chemicals which generate prooxidant states
A bioassay making use of submitochondrial particles to test for the presence of toxic substances which induce prooxidant states in vivo. The assay uses complex I of the electron transport enzymes...
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5196314 |
Process and reagent for the determination of substrates or enzyme activities
A process for the determination of substrate or enzyme activities by the use of a redox reaction as a measurement reaction is carried out in the presence of one or more additionally added...
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5196340 |
Enzyme electrode containing an enzyme and a coenzyme immobilized in separate layers of a membrane
An enzyme electrode comprising an enzyme and a coenzyme immobilized in at least one layer is provided. This electrode has an outer membrane preventing the coenzyme from dissipation and so...
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5190863 |
Composition for determining the presence or concentration of D-β-hydroxybutyrate
A composition, test device and method of determining the presence or concentration of ketone bodies, and specifically D-β-hydroxybutyrate, in a test sample are disclosed. The test device includes...
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5188941 |
Enzymatic determination of theophylline
The present invention provides a new methodology and test composition for determining the presence of theophylline in test samples. The methodology employs enzymes that utilize or recognize...
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5185249 |
Dry analytical element for assaying salicylate
A dry multilayer element for assaying salicylate is disclosed.
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5166049 |
Measurement of diaphorase activity and reagent therefor
A method for measuring diaphorase activity comprising mixing a sample comprising diaphorase with nitro blue tetrazolium, EDTA or a salt thereof, at least one of nicotinamide adenine dinucleotide...
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5162516 |
Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis
The alcohol dehydrogenase II gene from Zymomonas mobilis has been cloned and sequenced. This gene can be expressed at high levels in other organisms to produce acetaldehyde or to convert...
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5162203 |
Methods of measuring isozymes and isozyme classes of alcohol dehydrogenase
The present invention provides fluorescence-based methods for sensitively detecting total ADH activity in human sera and selectively measuring the activity of different classes of ADH in human sera...
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5158873 |
Method and reagent for determining LD-1 isoenzyme
A rapid and specific method and reagent for determining LD-1 isoenzyme in biological fluids by incorporating a chaotropic agent, such as sodium perchlorate, into an LDH assay reagent system. The...
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5156955 |
Nucleoside oxidase and assay method utilizing same
The present invention provides a novel nucleoside oxidase YT-1 serving as an enzyme for catalyzing oxidation reaction of a nucleoside, and oxidase being characterized in that the oxidase causes the...
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5141854 |
Enzymatic composition for ethanol assay
An enzymatic assay composition for ethanol determination is provided. A compound of the formula H 2 N--(CH 2 ) n --CHR--CH 2 --NH 2 where R is H, CH 3 , or OH and N is 0-4 acts as a...
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5141855 |
Signal amplifying cobalt (III) redox reagents and methods for the determination of analytes in aqueous fluids
Reagents and methods for the determination of analytes are disclosed, using water soluble cobalt(III) complexes and metallizable dyes to form cobalt(III) complexes of the metallizable dyes....
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5141853 |
Determination of ammonia levels in a sample
A method and kit for determining the amount of ammonia in a body fluid. The method and kit involve contacting the fluid with glutamate dehydrogenase, alpha-ketoglutarate and nicotinamide...
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5126275 |
Analytical method using tetrazolium salt indicators having a reflectance plateau
General classes of 2-thiazolyl tetrazolium salt compounds have been found to be characterized by a reflectance spectrum exhibiting an extended plateau above about 600-650 nm. Such compounds are...
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5126247 |
Method, system and devices for the assay and detection of biochemical molecules
Oxygen-independent methods, systems and devices for the enzymatic colorimetric assay and detection of biochemical analytes. Two systems are described, both of which produce less than one equivalent...
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5126245 |
Reagents for assay of gamma-glutamyltranspeptidase
A reagent for assaying γ-glutamyltranspeptidase (γ-GTP) which contains an amino acid dehydrogenase and a substrate specific to γ-glutamyltranspeptidase activity. The reagent can be used to assay...
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5124141 |
Method for diagnosing malaria
A diagnostic method for the detection of human malaria infections. Specifically, blood samples are combined with a reagent containing 3-acetyl pyridine adenine dinucleotide (APAD), a substrate...
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5122454 |
Assay method for lecithin-cholesterol acyltransferase
An assay with high sensitivity for activity of lecithin-cholesterol acyltransferase in blood for functional analysis of liver, by bringing the blood into contact with lecithin and free cholesterol...
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5122456 |
Amperometric method for the quantitative determination of 1,4-dihydronicotinamide adenine dinucleotide (NADH) in solution
A method is disclosed for the quantitative determination of 1,4-dihydronicotinamide adenisne dinucleotide (NADH) in solution. The method comprises contacting the NADH-containing solution with an...
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5116728 |
Reagents for CO.sub.2 detection
A stabilizer for maintaining a constant level of CO 2 in coenzyme containing enzymatic CO 2 diagnostic reagents is disclosed, comprising rate-limiting amounts of (a) the diagnostic reagents and...
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5116759 |
Reservoir chemical sensors
Single or multi-cell reservoir sensors with single illumination sources and one or more detectors per cell unit have an arrangement whereby a gaseous, vapor or liquid sample enters the cell body...
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5116732 |
Tetrazolium halide compounds and methods
Compounds of the formula DNB-TZ(P2) (P3) X, wherein TZ is a tetrazolium ring, DNB is 5-(2,4-dinitrophenyl), X is halide and P2 and P3 are each independently halophenyl, nitrophenyl or phenyl. The...
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5112741 |
Acetaldehyde trapping system
The invention provides a reagent system for the enzymatic determination of an oxidizable substrate in a fluid sample; the system includes an active amine trap for inactivating high concentrations...
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5112740 |
Carbon dioxide assay for body fluids comprising carbonic anhydrase
A multilayer element for assaying carbon dioxide is disclosed. The elememt comprises a spreading layer comprising acetazolamide, a reagent layer comprising phosphoenolpyruvate carboxylase and...
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5108905 |
Method of assaying magnesium in human body fluid
Disclosed is a method of assaying the content of Mg ions in a human body fluid such as serum, urine or saliva by the use of a reactant solution containing isocitrate dehydrogenase, NADP + ,...
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5108903 |
Reducible compounds which provide leuco dyes for analytical compositions, elements and methods of using same
Certain reducible compounds are useful in analytical compositions, elements and methods, for example for the detection of bacterial cells. These compounds comprise a moiety which provides a leuco...
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5094943 |
Process and reagent for the improved quantitative colorimetic determination of hydrogen peroxide
The present invention provides a process for the improved colorimetric determination of hydrogen peroxide as formed by a hydrogen peroxide-producing oxidase, by addition of a chromogenic system and...
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5089388 |
Antibodies for salicylate and their preparation
Conjugates of salicylic acid and certain poly(amino acids), which are either antigenic or enzymes, are provided. Antibodies raised against the antigenic poly(amino acids) and the enzyme conjugates...
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5085989 |
Method and reagent for quantitative analysis of 3-oxo-5β-steroid
A quantitative analysis of 3-oxo-5β-steroid is carried out by acting 3-oxo-5β-steroidΔ 4 -dehydrogenase on a sample in the presence of a reducing chromophoric agent, and measuring the optical...
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5082770 |
Method for quantitative determination of polyamines
A method for the quantitative determination of polyamines, which comprises allowing a polyamine oxidizing enzyme, an ω-aminoalkylaldehyde dehydrogenase, an oxidized nicotinamide coenzyme and, as...
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5081016 |
Method for measuring the amount of polyamines in a biological sample
A method for measuring the amount of polyamines in a biological sample is provided. The method comprises utilizing diamine oxidase and/or polyamine oxidase, aminoalkylaldehyde dehydrogenase and NAD...
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5081014 |
Method of measuring a co-enzyme
The method makes use of the action of the co-enzyme in the production of H 2 O 2 associated with the reaction of a hydroxylase with a decoupling agent in the presence of air or oxygen, the H 2 O...
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5068190 |
N-acetylhexosamine-dehydrogenase, process for producing same, method for the quantitative analysis of N-acetylglucosamine or N-acetylgalactosamine using same and kit for use in the quantitative analysis
N-Acetylhexosamine-dehydrogenase which takes off hydrogen from N-acetylglucosamine or N-acetylgalactosamine to convert them to N-acetylglucosaminolactone or N-acetylgalactosaminolactone,...
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5059526 |
Dry multilayer analytical element for analysis of enzymes or triglycerides
A stable dry multi-layer analytical element for enzyme or triglyceride analysis in a fluid sample is prepared containing first and second layers on a support. The first layer contains a tetrazolium...
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5047322 |
Use of dry analytical elements to determine analytes
Dry transparent analytical elements can be used to determine analytes which have been separated by electrically induced migration through a solid medium, e.g. by electrophoresis, or which are...
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5041373 |
Method for determining periodontal disease
An objective method for determining the presence of active periodontal disease in mammals, comprising assaying cervicular fluid for the presence of elevated levels of the enzyme aspartate...
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5037739 |
N-acetylmannosamine dehydrogenase, process for its production, method for quantitatively analyzing N-acetylmannosamine or sialic acid, and kit for the quantitative analysis
N-Acetylmannosamine dehydrogenase which takes off hydrogen from N-acetylmannosamine to convert it into N-acetylmannosaminolactone and, at the same time, reduces coenzyme NAD into NADH. This enzyme...
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5037738 |
Simultaneous assay for glucose and urea
A method for the simultaneous determination of glucose and urea in a specimen with a single reagent system. A reagent system containing a reactant for each of glucose and urea to be determined is...
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5035995 |
Test method involving substance-conjugated complement component C1q
A substance-conjugated complement component C1q is provided. A substance such as signal emitting substances or cell function regulating substances is conjugated via a sulfur atom to at least one...
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