AcclaimIP-ad

Match Document Document Title
4157279 Process for the determination of at least one of the isoenzymes of lactatedehydrogenase  
Process for the determination of at least one of the isoenzymes of lactatedehydrogenase by visually observing and recording the conversion of lactate and nicotinamide-adenine-dinucleotide to...
4153511 Stabilized liquid coenzyme compositions  
Labile coenzymes, such as reduced nicotinamide-adenide dinucleotide (NADH2), are stabilized by forming a composition of the coenzyme with an organic solvent such as 1,2 propanediol in the presence...
4142938 Determination of triglycerides and glycerol  
Triglycerides in biological fluids containing interfering substances, such as phospholipids, are determined by hydrolyzing the triglycerides to glycerol in a short time span at moderate...
4121975 Pretreatment of samples for polyiodothyronine assays  
Composition and method are provided for the pretreatment of serum prior to a thyroxine assay. Prior to carrying out of the assay, particularly an immunoassay or competitive protein-binding assay,...
4120755 Kinetic method for determination of glucose concentrations with glucose dehydrogenase  
The range of linearity of the kinetic method for the determination of glucose concentrations using a reagent of the type comprising a buffer, a pyridine coenzyme, and glucose dehydrogenase, is...
4116635 General purpose in vitro anticoagulant  
A process for testing a blood sample as for coagulation, hematology, chemistries, enzymes, electrolytes and trace metals which involves the addition of a particular anticoagulant. The...
4097338 Fluorimetric demonstration and determination of a reduced coenzyme or derivative in an aqueous system  
The present invention relates to a rapid and sensitive method for the determination of a reduced coenzyme, particularly NADH, NADPH and derivatives thereof, in that the fluorescence of such a...
4097336 Reagent system for beta-amylase assay  
A reagent system for beta-amylase assay comprising:A polysaccharide having glucose molecules primarily connected through alpha 1,4 linkagesPhosphate ionsMaltose phosphorylaseA co-enzyme selected...
4085009 Methods for determination of enzyme reactions  
A method, for microcoulometric determination either of the activity of an enzyme in a liquid sample or the concentration of a substrate for a reaction involving a change in a coenzyme from a first...
4080263 Process and reagent for the rapid quantitative determination of lactate or alanine  
L-lactate is quantitatively determined by the reaction thereof with nicotinamide-adenine-dinucleotide (NAD) in the presence of lactate dehydrogenase (LDH) with the formation of reduced NAD (NADH)...
4071020 Apparatus and methods for performing in-vivo measurements of enzyme activity  
There are disclosed apparatus and methods for performing in-vivo measurements of enzyme kinetics on the epidermis and mucous membranes of a subject. One or more reactants are placed on a...
4071407 Novel maltase enzyme produced by a new yeast strain  
A maltase enzyme is prepared by growing a strain of Saccharomyces, ATCC 20 488, on a medium containing maltose or malt extract and has been found to be useful in assays for amylase.
4067775 Process and composition for determining the activity of creatinekinase-MB  
A process for determination of CK-MB activity in a sample of a body fluid comprising the steps of incubating the sample, optionally in the presence of CK substrates, with antibodies which inhibit...
4066512 Biologically active membrane material  
A composite membrane structure for immobilizing biologically active materials, such as enzymes, is formed by coating a microporous polymeric membrane with a thin layer of an inert proteinaceous...
4066508 Process and reagent for determining triglycerides  
Triglycerides are determined by enzymatic saponification with lipase in a buffer in the presence of a carboxylesterase and an alkali metal or alkaline earth metal (10-15 carbon atom) alkyl...
4065358 Apparatus for producing reactions in colorimetric cells  
An apparatus according to which a sample and a reagent are mixed together at a given temperature in a colorimetric cell to enable a property of the sample to be determined in an instrument such as...
4059407 Disposable chemical indicators  
A disposable indicator is disclosed which is useful for the measurement of ketones, proteins including albumin and uric acid in biological fluids. The instrument registers the concentration of...
4056485 Stable colored reference standard for enzymatic determinations  
A stable, colored reference standard suitable for use in diagnostic determinations involving enzymatic reactions in which colorless 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride...
4056442 Lipase composition for glycerol ester determination  
A synergistic composition of microbial lipases from Rhizopus arrhizirs and Candida cylindracea is used for the hydrolysis of glycerol esters such as triglycerides in the determination of glycerol...
4046636 Diazepam enzyme conjugates  
Novel compositions are provided for the determination of one or a group of organic materials (hereinafter referred to as "ligands"), where the compositions have a ligand or ligand counterfeit...
4046634 Assay of isoenzymes by ion exchange chromatography  
A simple, rapid anion-exchange column chromatographic technique is used to determine the presence of fractions of proteins and enzymes in human serum by separating the fractions on the basis of...
4045297 Triglycerides determination method  
A method of determining triglycerides in biological fluids which comprises reacting a biological fluid sample with a lipolytic enzyme to liberate free glycerol, reacting said free glycerol with a...
4043872 Polyiodothyronine immunoassay  
Method and enzyme conjugates are provided for determining physiological amounts of polyiodothyronine in physiological fluid, particularly serum.A homogeneous enzyme immunoassay is provided...
4043759 Method of determining methotrexate  
A radioassay system for the determination of methotrexate in biological fluids based on the competitive binding of labeled and unlabeled methotrexate to the enzyme dihydrofolate reductase. Samples...
4042462 Creatine phosphokinase determination method  
A method of determining creatine phosphokinase activity in biological fluids comprising reacting a biological fluid sample with a coupled enzyme series in an oxygenated aqueous solution containing...
4040907 Iodothyronine enzyme conjugates  
Polyiodothyronine conjugates to enzymes are provided which find use in the determination of polyiodothyronine compounds, particularly thyroxine, normally in physiological fluids, such as serum....
4039385 Cardiac glycoside enzyme conjugates  
Enzyme conjugates are prepared of cardiac glycosides and aglycones for use in homogeneous enzyme immunoassays. The conjugates retain a substantial degree of the original activity of the enzyme and...
4038146 Method of determination of serum triglycerides and reagents  
A serum sample is added to a reaction liquid obtained by adding an aqueous solution of a mixture of a tetrazolium compound and phenazine methosulfate to an enzyme-coenzyme reagent comprising LPL,...
4036697 Kinetic assay for alpha-amylase  
A kinetic assay is provided for measuring alpha-amylase content in aqueous solutions. The assay is based on the following reactions: alpha-1,4 linked glucan alpha-amylase alpha-maltose...
4035239 Enzymic assay for acetate ion  
An improved enzymic assay and assay composition for acetate ion in a biological fluid (e.g., blood serum or tissue extract) or a solution of fruit juice origin (e.g., wine). The acetate ion is...
4026767 Test procedure for microorganisms in blood  
A method for indicating the presence of microorganisms in blood or other fluids comprising introducing the material to be tested into a nutrient medium containing a ditetrazolium chloride which...
4024021 Determination of glutamate and glutamic transaminases  
Glutamate and glutamic transaminases are determined by mixing a substrate-reagent composition that is essentially free of ammonia or ammonium ions with a specimen to be analyzed, incubating the...
4019961 Analytical enzymatic determination  
An analytical process for enzymatic determination of a substrate in biological fluids. The fluid sample is reacted first in the absence of the specific kinase but in the presence of an NADH --...
4017365 Method for determining enzyme activity  
Difficulty in using the Karmen method due to lipemic turbidity and substances absorbing ultraviolet rays in serum can be prevented by reducing the amount of GOT or GPT-containing sample and adding...
4013513 Ion exchange chromatographic isoenzyme separation and isolation  
A process of separating and isolating fractions containing isoenzymes from a sample of tissue extract or blood serum comprising ion-exchange column chromatographically separating the sample...
4012285 Analysis of isoenzyme patterns  
Isoenzyme patterns of an enzyme in biological fluids, extracts, and/or excretions are quantitatively determined by precipitating at least 90% of an isoenzyme antigen with a corresponding...
4012286 Determination of creatine phosphokinase in body fluids  
An improved method and compositions useful for determining the creatine phosphokinase activity of body fluids suitable for use on clinical colorimeters which involves pre-activation of the...
4012287 Method and reagent for the quantitative analysis of triglycerides  
A reagent and method for the quantitative of serum triglycerides which involves the alcoholic saponification of serum lipides using a low molecular weight chlorinated hydrocarbon as a catalyst....
4006061 Lactate dehydrogenase determination method  
A method of determining lactate dehydrogenase activity in biological fluids comprising reacting a biological fluid sample with a coupled enzyme series in an oxygenated aqueous solution containing...
4003795 Process for the determination of at least one of the isoenzymes of lactatendehydrogenase  
Process for the determination of at least one of the isoenzymes of lactatedehydrogenase by visually observing and recording the conversion of lactate and nicotinamideadenine-dinucleotide to...
4001089 Method for determination of triglycerides and glycerol  
A novel reagent and method for colorimetrically determining the concentration of triglycerides in biological fluids, and for rapid determination of glycerol are described. Triglycerides are...
4000042 Diagnostic reagent for the determination of amylase  
Disclosed herein is a diagnostic agent for determining the α-amylase content of a sample comprising a first compound selected from the group consisting of maltotetraose, maltopentaose, and...
3985621 Stopping agents for enzyme reactions of dehydrogenase systems  
New application of an aqueous alkali solution of cyclohexylaminopropanesulfonic acid, said acid being contained therein with a specific range of concentration and said solution having a specific...
3977944 Enzyme-kinetic determination of the concentration of a substrate  
Enzyme-kinetic determination of the concentration of an enzyme substrate which is acted on during an enzymatic reaction is carried out by adding at least one competitive inhibitor to an enzymatic...
3975237 Compounds for enzyme amplification assay - - ecgonine analogs  
Novel biological assay method for determining the presence enzyme a specific organic material by employing a modified enayme for amplification. By employing receptors specific for one or a group...
3974037 Process for measuring carbon dioxide content of the body fluid  
A process for measuring the carbon dioxide content of a body fluid comprising the steps of mixing measured amounts of the body fluid with excess amounts of a substrate/enzyme combination selected...
3966897 Medium for use in bioassay and method of using same  
A hydratable or hydrated gel medium for use in bioassay of a test substance contains fixed in a localized zone a reagent for the test substance. Assays are conducted rapidly by diffusing the test...
3964974 Enzymatic determination of glucose  
Glucose is rapidly ad quantitatively determined with an analytical agent containing glucose dehydrogenase having an activity of at least 2 μ/mg and NADH2 oxidase activity less than 0.1%, a...
3959074 Vaccine production  
A process for the controlled manufacture of cells and vaccines in which reproducable real time quantitative assays are utilized to ascertain the physical and chemical state of the system, in order...
3957748 Nicotinamide-adenine-dinucleotide chemically coupled to water-insoluble carriers  
Nicotinamide-adenine-dinucleotide (NAD) can be coupled chemically to an essentially water-insoluble carrier material selected from the group consisting of glass particles having a diazotizable...