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4350760 Method for the separation of a protein substance from a solution containing the same by affinity filtration and application of said method to enzymatic assays  
The invention relates to a method for the selective separation of a given protein substance having an affinity for a ligand from other substances some of them having the same affinity for the...
4350762 Aminopyrine improved Trinder's reagent and dosing process for hydrogen peroxide from enzymatic oxidation of metabolic substrata with the same  
An aminopyrine-improved Trinder's reagent is disclosed to be used for the dosing of hydrogen peroxide from enzymatic oxidation of metabolic substrata, such as glucose, cholesterol, uric acid,...
4349625 Method for assaying fatty acids  
There is disclosed a method for assaying fatty acids in a system containing albumin together with the fatty acids using acyl-CoA synthetase, characterized by performing the assay in the presence...
4342827 Production of enzymes  
Glycerol dehydrogenase enzymes having exeptionally good thermal stability are produced by culturing novel strains of Bacillus stearothermophilus. Procedures for deriving and identifying suitable...
4338395 Method for the analysis of triglycerides  
Method of analyzing for triglycerides in a biological fluid is disclosed which eliminates interferences arising from endogenous glycerol and pyruvate and the need for parallel blank correction.
4329425 Method for determination of transaminases and relative diagnostic kit  
A kinetic UV method of determining GOT and GPT by reacting transaminase with a substrate uses L-cysteinsulfinic acid and L-alanine as the substrate and lactic dehydrogenase as the revealing enzyme.
4325832 Enzyme reference composition  
A stable enzyme reference composition having an excellent shelf-life. The composition comprises at least one enzyme of known value; about 20 to about 40 weight percent of at least one alkylene...
4324687 Blood biochemistry control standard  
A blood biochemistry control standard for the quality control of the analytical measurement of blood biochemistry components is disclosed. The control standard comprises an aqueous suspension of...
4312945 Method and apparatus of measuring the specific activity of dehydrogenases in tissue  
In a method of measuring the specific activity of dehydrogenases by using a specimen prepared through a first procedure of causing dehydrogenases in tissue flake to be measured to be reacted with...
4311791 Automated kinetic determination of lactate dehydrogenase isoenzymes in serum  
A diagnostic method is disclosed, based on the automated kinetic determination of LDH and MDH isoenzymes in serum, which utilizes the comparison of two rates of LDH and MDH activity at different...
4310625 Stabilized liquid enzyme compositions for diagnostic determinations  
Labile enzymes for use in biological diagnostic determinations are stabilized for long terms by forming a solution of the enzyme in an aqueous media containing at least 20% organic solvent such as...
4310624 Stabilized liquid coenzyme compositions for diagnostic determinations  
Labile coenzymes, such as reduced nicotinamide-adenide dinucleotide (NADH2), used in biological diagnostic determinations are stabilized by forming a composition of the coenzyme with an organic...
4309502 Enzymatic assay for glycerol and triglycerides and a reagent for use therein  
An assay which is capable of determining the endogenous glycerol and endogenous triglycerides content of a sample without the necessity of performing a separate glycerol assay. The assay of the...
4309184 Method of determining food or chemical allergy and intolerance  
Food or chemical allergy and intolerance is determined by comparing an analysis for a given analyzable constituent of a polymorphonuclear leukocyte with and without incubation with the...
4299669 Method for determining the concentration of an L-amino acid in fermentation  
Electrochemical method for determining the concentration of a carbon source and L-amino acid in a fermentation medium or cultured broth by contacting a microbial electrode consisting of an...
4278760 Method and composition for determining an oxidized pyridine co-enzyme  
A method for the quantitative determination of an oxidized pyridine co-enzyme which comprises reacting a sample containing oxidized pyridine co-enzyme in the presence of its specific alcohol...
4277562 Stabilized liquid enzyme and coenzyme compositions  
A multi-reagent for use in stabilized liquid form is prepared containing three separate reagent solutions which can be combined in proper amounts to form a working composition for a biological...
4273869 Cystic fibrosis detection method  
A method of diagnosing cystic fibrosis, identifying carriers for cystic fibrosis, and non-carriers or "normal" persons. Heretofore, no carrier or prenatal detection procedure for cystic fibrosis...
4273870 Method and composition for the determination of glycerol  
Method and composition for the determination of glycerol using glycerin dehydrogenase, an electron transfer agent, NAD and a tetrazolium salt and, in addition, at least one microgram of zinc, in...
4271265 Method and reagent for the determination of glutamate-oxalacetate transaminase and glutamate-pyruvate transaminase  
In a method for the determination of glutamate-oxalacetate transaminase or glutamate-pyruvate transaminase by the reaction of oxalacetate or pyruvate with glutamate, with formation of...
4266022 Process for the determination of at least one of the iso-enzymes of lactatedehydrogenase  
Process for the determination of at least one of the isoenzymes of lactatedehydrogenase by visually observing and recording the conversion of lactate and nicotinamide-adenine-dinucleotide to...
4261893 Bis-phthalimide intermediates  
Bis-phthalimide intermediates of the formula: ##STR1## wherein one of R9 and R10 is hydrogen and the other is --NR11 R12 ; R11 is hydrogen or straight stain alkyl containing 1-4 carbon atoms and...
4260681 Reagent system and method for assaying peptidase enzymes  
A reagent system for assaying a biological specimen for a peptidase enzyme comprising a peptide substrate in which the C-terminal amino acid is dehydroalanine, the enzyme lactate dehydrogenase,...
4260392 Method and apparatus for obtaining an aliquot of a liquid in a gel medium  
There is disclosed a method and apparatus for obtaining a precise aliquot of a sample by diffusing the sample into a gel mass for a controlled period of time. The sample may comprise a liquid...
4259440 Hydrolysis and assay of triglycerides  
A method and composition for the hydrolysis and assay of triglycerides are disclosed. The method includes the steps of adding lipase and cholesterol esterase to a triglyceride in combination with...
4258131 Method of fractional quantitative determination of isoenzyme of lactic dehydrogenase  
1,6-Dihydro nicotinamide adenine dinucleotide inhibits both H-type lactic dehydrogenase and M-type lactic dehydrogenase which are isoenzymes of lactic dehydrogenase, but the degree of inhibition...
4254222 Semi-quantitative assay of lactic acid and β-hydroxy butyrate  
There is provided a procedure for determining the concentration of a metabolic acid in a biological fluid. The method involves use of a tetrazolium salt, a pyridine nucleotide, an electron...
4250255 Assay method for isoenzyme activity  
The activity of an isoenzyme in a sample is determined by assaying for the isoenzyme in the presence of a predetermined concentration of an ionic amphiphile having a hydrophilic and a hydrophobic...
4247630 Method and reagent for the determination of uric acid  
The invention relates to an improvement in a conventional method for the determination of uric acid involving uricase/catalase/aldehyde dehydrogenase, resulting in the formation of produced...
4247633 Reagent for colorimetric determination of creative phosphokinase  
An all-in-one reagent is provided for the quantitative colorimetric determination of creatine phosphokinase in blood serum or plasma wherein the tetrazolium dye,...
4246084 Electrophoretic technique for assaying the relative distribution of lactate dehydrogenase isoenztimes and buffers for use therein  
An electrophoretic technique for assaying the relative distribution of lactate dehydrogenase (LD) isoenzymes characterized in that the electrophoretic and/or substrate buffer employed therein...
4245041 Triglycerides assay and reagents therefor  
An improved triglyceride assay and reagent system which provides the introduction of iron into a reaction mixture in which glycerol from hydrolyzed triglycerides is coupled to a NAD/NADH indicator...
4243753 Apparatus for enzyme detection  
An apparatus is disclosed for enzyme detection that is particularly well suited for high performance liquid chromatography. The apparatus is basically a flow-through enzyme detector that is...
4242446 Method for determining a substance in a biological fluid and reagent combination for use in the method  
An improvement in a method for determining a substance present in a biological fluid by oxidizing a compound produced in the course of the determination in the presence of a dehydrogenase enzyme...
4241179 Method for determining a transaminase in a biological fluid and reagent combination for use in the method  
An improvement in a method for determining glutamate oxalacetate transaminase or glutamate pyruvate transaminase present in a biological fluid, in which method L-glutamate is produced from...
4237221 Process for obtaining maltose phosphorylase and β-phosphoglucomutase and method of determining α-amylase using same  
The present invention provides a process for obtaining maltose phosphorylase and/or β-phosphoglucomutase from micro-organisms, wherein the starting material used is selected from Lactobacillus...
4235962 Combination kit for transaminase assay of a body fluid  
A kit for the determination of transaminase present in body fluid having a first reagent containing aspartate, a second reagent containing alanine, and a third reagent containing 2-oxoglutarate...
4229527 Process and reagent for the kinetic determination of enzyme substrates  
Kinetic determination of enzyme substrates by means of coupled reactions comprising selecting the reaction parameters so that the most specific part reaction of a reaction sequence becomes...
4229529 Process for determining formate and reagent therefor  
Formate, or compounds convertible into formate, is determined by reacting same in the presence of formate dehydrogenase (FDH) and of a hydrogen acceptor, e.g., NAD, wherein the formate...
4224406 Immunochemical LDH1 assay  
LDH1 levels in serum samples can be rapidly and accurately assayed by a novel immunochemical technique. In such procedure the serum sample is treated with soluble antibodies against the M subunit...
4223090 Reagents for the enzymatic determination of triglycerides  
A method of determining triglycerides in biological fluids according to an enzymatic reaction. In the reaction, a biological fluid is added to an enzyme reagent comprising a lipase, glycerol...
4220714 Composition for inhibiting adenylate-kinase and its use  
A method and a combination of reagents are disclosed for determining the creatine kinase activity by inhibiting the action of creatine kinase, the improvement consisting in that adenosine - 5'-...
4218536 Process-stable co-enzyme NAD solution  
A stable oxidized co-enzyme NAD reagent, ready for use in photometric assay of body fluids is prepared by dissolving the co-enzyme NAD in a neutral organic solvent such as glycol (antifreeze). The...
4215197 Test means and method for creatinine determination  
Test means, such as a composition or a test device incorporating the composition, method of making the test device and process for determination of creatinine using the test means are disclosed....
4212939 Substrate solution for carboxylic ester hydrolase determination  
A clear, stable aqueous reagent solution comprising a mixture of a water insoluble vinyl ester of an alkyl, alkenyl, or alkyl-aryl carboxylic acid, nonionic detergents, inorganic salts, enzyme...
4202938 Procedure for the quantitative determination of hydrogen peroxide concentration in aqueous solutions  
A novel procedure for the quantitative determination of hydrogen peroxide concentration in aqueous solutions is described. This is characterized by reacting hydrogen peroxide with an alcohol in...
4197369 Method for measuring reactant concentrations and quantities  
A method and apparatus is provided for determining the concentration or quantity of enzyme molecules, microorganisms or substrate molecules by monitoring directly the concentration and/or the...
4168207 TBG assay  
Method and compositions are provided for determining the thyroxine binding capacity of serum, the value finding use in determinations of free thyroxine index. A competitive assay is performed...
4166765 Detecting Neisseria bacteria  
Bacteria of the genus Neisseria can be detected in a sample by testing for the presence of an enzyme capable of oxidizing 1,2-propanediol and reducing NAD (nicotinamide-adenine-dinucleotide). The...
4162194 Kinetic assay for acid phosphotase and composition therefore  
A kinetic assay is provided for determining the acid phosphatase in an aqueous solution by measuring the amount of of reduced coenzyme produced. The assay is based on the following coupled...