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4629696 Process for the selective production of reduced oxygen species  
Process for the selective production of the reduced oxygen species superoxide, hydrogen peroxide and hydroxyl radicals, wherein oxygen is reduced with NAD(P)H in the presence of an...
4629697 Test system and procedure for the determination of NAD (P) H  
A test system having an extended range of measurement and an appropriate procedure for the determination of NAD(P)H or of substrates or enzymes which react to form or consume NAD(P)H in fluids is...
4629691 Tricyclic antidepressant conjugates with antigens and enzymes  
Tricyclic antidepressant functionalized compounds are provided for conjugation through a side chain to antigenic compounds, particularly poly(amino acids), and enzymes. The antigenic conjugates...
4629693 Sensitivity in fluorescence assays in icteric samples  
Enhanced sensitivity in fluorescent assays employing NADH or NADPH is obtained, particularly in icteric samples, by employing excitation light at a wave length range below about 400 nm and reading...
4622296 Process for measuring activity of dehydrogenase employing a reaction stopper  
In a process for measuring the activity of a dehydrogenase or the amount of substrate reacted in the presence of the dehydrogenase using NAD or NADP as coenzyme and a tetrazolium salt as a...
4622294 Liposome immunoassay reagent and method  
A liposome assay reagent for determination of an analyte in a homogeneous immunoassay. The reagent includes a suspension of oligolamellar lipid vesicles containing encapsulated glucose-6-phosphate...
4621048 Reagents containing an anti-ligand bound to an anti-enzyme and methods for employing said reagents in an immunoassy  
The invention relates to a method and novel reagents for detecting a ligand in a biological sample. In the method, the sample is combined with (i) an enzyme or an enzyme bound to a first...
4604347 Analytical element for dry analysis  
An analytical element containing at least one reagent for dry analysis of analyte in a liquid sample, which contains a nondiffusive acid and a nondiffusive base in different phases, being capable...
4598042 Immunoassay with an increasing cyclic detection system  
An immunoassay uses an antibody or antigen -enzyme conjugate which converts a precursor to a cycling agent used in a cycling detection system. The amount of cycling agent is continually increased...
4592996 Process for determining reduced form coenzymes  
A reduced form coenzyme can be determined by acting a peroxidase on a reduced form coenzyme in the presence of metal ions to generate hydrogen peroxide and measuring the amount of hydrogen...
4591555 Malate dehydrogenase method  
A method of detection of neoplastic tissue comprising, providing a first solution, the first solution comprising cytosol malate dehydrogenase from a mammalian test tissue, determining a first rate...
4590164 Process for the preparation of a glucose-free hemoglobin standard  
A process for the preparation of a glucose-free glycosylated hemoglobin standard in which hemoglobin with a known content of glycosylated hemoglobin HbAIa+b+c is converted to cyanmethemoglobin by...
4576913 NAD(P)-Independent glycerol dehydrogenase, a process for the preparation thereof and the use thereof for the determination of glycerol and triglycerides  
The present invention provides a new NAD(P)-independent glycerol dehydrogenase and a process for obtaining it, wherein a micro-organism of the species Pseudomonas, Serratia, Klebsiella, Erwinia,...
4575488 Interference free transaminase assay  
Compositions for pyridoxal-5'-phosphate (PLP) activated transaminase determinations are provided, in which interference due to PLP is avoided. Amino-functionalized polymers bind PLP which is not...
4556634 High sensitivity assay method  
A high sensitivity assay method of the coenzyme cyclic assay type, eliminates the need for a separate indication reaction, by using the cycling reaction itself as the indication reaction,...
4550079 Determination of L(+)-tartrate or D(+)-malate with L(+)-tartrate dehydrogenase  
A L(+)-tartrate dehydrogenase with L(+)-tartrate dehydrogenase and D(+)-malate dehydrogenase (decarboxylating) activity, which can be obtained from Rhodopseudomonas sphaeroides, and also a process...
4550077 β-Amylase determination  
A reagent suitable for α-amylase determinations. The reagent contains a modified, blocked amylaceous polysaccharide substrate, e.g., starch glyolate, an exating by amylose, a buffer and an...
4547460 Multizone analytical element and method for analyte determination  
A multizone analytical element for the determination of an analyte in an aqueous liquid comprises a registration zone and a porous spreading zone to receive and transport the aqueous liquid. The...
4547465 Analytical element having improved spreading zone and method of use  
A dry element capable of use for the analysis or transport of liquids comprises an isotropically porous zone which comprises a blush polymer having dispersed therein a particulate material and a...
4546077 Leucine dehydrogenase and a process for production thereof  
Leucine dehydrogenase is disclosed which retains 80% or more of the activity even after it is incubated in a buffer solution at about 70° C. for 10 minutes, as compared to the activity prior to...
4544631 Process and reagent for the determination of α-amylase  
α-Amylase is determined by the enzymatic splitting of an α-amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula...
4517287 Method and reagent for the enzymatic determination of enzyme substrates  
The present invention provides a reagent for the enzymatic determination of an enzyme substrate, containing a system for the determination of the substrate, which comprises at least one enzyme, at...
4505756 Alpha-amylase assay and substrates for use therein  
A maltodextrin phosphorylase limit dextrin in the presence of maltodextrin phosphorylase and inorganic phosphate, is used as a substrate for alpha-amylase, which initiates a series of enzymatic...
4491631 Assay method for lipid component, assay composition, and process for production of enzyme used therefor  
An enzyme having enoyl-CoA hydratase activity, 3-hydroxyacyl-CoA dehydrogenase activity and 3-ketoacyl-CoA thiolase activity, all in the same enzyme, is produced by culturing the microorganism...
4490465 Coupled enzyme systems for determination of dissolved substances  
A test system having an extended range of measurement and a corresponding procedure for the determination of substances in liquids contains several enzymes or enzyme systems together which,...
4485174 Hemoglobin-based blood gas control  
Blood gas control containing hemoglobin, a pH buffering agent, and a reduced pyridine nucleotide capable of participating in a reaction which reduces methemoglobin to normal hemoglobin.
4483922 Inactivation of enzymes  
A method for inactivating enzymes, particularly enzymes found in human serum. The method comprises reacting the enzyme in a suitable medium, e.g. human serum, with an inactivating amount of...
4477566 Method for determination of cytoplasmatic and mitochondrial isoenzymes of glutamic-oxalacetic transaminase in human serum or plasma  
The determination of the single enzymatic activities of the cytoplasmatic and mitochondrial isoenzymes of Glutamic-Oxalacetic Transaminase in samples of serum or plasma is effected by determining...
4476222 Test piece for quantitative analysis of substances in body fluids  
A testing piece for quantitative analysis of a substance in a body fluid, particularly in blood, said testing piece comprising an absorbent carrier containing in it reagents for measuring the...
4474878 Sandwich EIA for antigen associated with hepatitis  
A sensitive direct immunoassay system is provided for the detection of an antigen associated with hepatitis in body fluids. A single antibody which reacts with a hepatitis antigen or antigens and...
4450232 Incorporation of pyridoxal phosphate in dry analytical elements for the determination of enzymes  
Enzymes which are activated by pyridoxal phosphate are assayed in a dry analytical element which includes a high coverage of pyridoxal phosphate. The coverage of pyridoxal phosphate is such that...
4446231 Immunoassay using an amplified cyclic detection system  
An immunoassay is disclosed where the label is an enzyme which converts a precursor into a cycling factor which in turn is interconverted in a cycling detection system. The detection system is...
4438199 Measuring composition  
An analytical composition comprises glucokinase and glucose-6-phosphate dehydrogenase.
4427771 Assay method for amylase activity and method of producing maltose dehydrogenase for use therein  
An assay method for amylase activity in a biological specimen such as serum, saliva or urine. The enzyme amylase in the specimen is used to decompose a substrate which is a glucose polymer having...
4425427 Simultaneous, kinetic, spectrophotometric analysis of blood serum for multiple components  
Method, kits and reagents for the simultaneous, kinetic spectrophotometric analysis of blood serum samples for multiple components. Pairs of components which may be simultaneously analyzed are:...
4416983 Determination of NAD(P)H or salicylate  
The present invention provides a process for the determination of NAD(P)H or of salicylate, wherein, in a NAD(P)H-dependent reaction, salicylate is decarboxylated by salicylate hydroxylase and a...
4415655 Electrophoretic separation of isoenzymes utilizing a stable polyacrylamide system  
A method for separating isoenzymes is disclosed. The method involves polyacrylamide gel electrophoresis in a buffer solution of a salt of 2-amino-2-methyl-1,3-propanediol at a pH of about 6.4 to...
4409328 Method and reagent for the determination of glycerol  
For the determination of glycerol, the latter is incubated with galactose oxidase in an aqueous medium in the presence of oxygen and either the oxygen consumption or the amount of H2 O2 or...
4397952 Process for obtaining glucose dehydrogenase and micro-organism therefor  
The present invention provides a process for obtaining glucose dehydrogenase by culturing a micro-organism in an appropriate nutrient medium and obtaining the enzyme from the cell mass or from the...
4394444 Cofactor indicator compositions  
A composition, test device, method of making a test device and process for determination of an analyte in a liquid sample are disclosed. More particularly, there is provided a composition for the...
4394449 Stabilization of coenzymes in aqueous solution  
A stabilized aqueous coenzyme solution is disclosed for use in the clinical assay of a selected biological constituent through the enzymic reaction of an enzyme with a selective substrate for the...
4384936 System for determining the concentration of an L-amino acid in fermentation  
Electrochemical method for determining the concentration of a carbon source and L-amino acid in a fermentation medium or cultured broth by contacting a microbial electrode consisting of an...
4379840 Quantitative analysis of uric acid  
This invention relates to a process for determining the uric acid content of biological material by contacting the biological material with a reagent composition comprised of uricase, catalase,...
4371611 Enzymatic diagnostic composition  
Disclosed herein is a method for binding enzymes comprising reacting either a synthetic or a natural polymer containing carboxyl and amino groups with an excess of a urethane prepolymer followed...
4368261 Method and reagent for the determination of triglycerides  
A method for the determination of triglycerides by ester cleavage utilizing lipase and optionally esterase with the formation of fatty acids and glycerol, phosphorylation of the glycerol with...
4361648 Color fixed chromogenic analytical element  
Analytical element method of making the analytical element and process for determining an analyte in a body fluid sample are disclosed. More particularly, the method of preparing the element...
4357420 Bioluminescence methods for enzymatic determinations  
An enzymatic method for continuous, on-line and rapid detection of diagnostically useful biomarkers, which are symptomatic of disease or trauma-related tissue damage, is disclosed. The method is...
4352885 Preparation of a novel NADP linked alcohol-aldehyde/ketone oxidoreductase from thermophilic anaerobic bacteria for analytical and commercial use  
A partially purified NADP specific thermostable alcohol, aldehyde/ketone oxidoreductase is prepared which can react with a wide range of alcohols, ketones and aldehydes. The enzyme has a unique...
4352881 Method of measuring creatine kinase activity  
A method and composition for use therein for measuring the activity of creatine kinase (hereinafter referred to as "CK") having good storage stability is disclosed. More particularly, a...
4351899 Semi-quantitative assay of metabolic acids  
A test indicator determines concentration, in a biological fluid, of a metabolic acid, such as beta-hydroxybutyrate or lactic acid. The indicator includes a test surface containing the dried...