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6852512 |
Expression vectors for production of foreign proteins as soluble forms
The present invention relates to novel expression vectors which can produce foreign proteins as soluble forms by using lysyl-tRNA synthetase and a process for preparing foreign proteins by using...
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6849593 |
Bifidogenic peptides
Pepetides having bifidogenic properties are obtainable by the process of adding proteases to cow'milk or human milk, followed by incubation, centrifugation, acidification, purification by reverse...
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6821755 |
Preparation of a recombinant protein in a prokaryotic host cell
The invention relates to advantageous processes for preparing heterologous proteins in prokaryotic host cells by improved codon use and/or expression of tRNAs which code for codons occurring rarely...
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6806047 |
Methods and compositions for amplifying detectable signals in specific binding assays
Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In...
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6783935 |
Genetic polymorphism of MxA protein and use thereof
The present invention provides polynucleotides having base sequences of sequence Nos. 1 to 4, as polynucleotides having polymorphism sites capable of being useful indicators for prediction of...
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6774213 |
Mutants of MAW motifs of RecA protein homologs, methods of making them, and their uses
RecA protein mutants and RecA homolog protein mutants which contain one or more mutations in the MAW motif are presented. The mutants rely on replacement of wildtype amino acid residues in the MAW...
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6770457 |
Process of purifying angiogenesis inhibitors
The present invention relates to process of purifying the angiogensis inhibitor proteins expressed in E. coli and to the use of recombinant kringle 1-3 (; greenstatin) purified by the above...
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6770443 |
Method and apparatus for mass spectrometric genotyping
Methods and equipment are provided for the mass spectrometric measurement of a large number of genotyping profiles, each formed by several tens to several hundreds of SNPs (single nucleotide...
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6756222 |
Escherichia coli having accession No. PTA 1579 and its use to produce polyhydroxybutyrate
The present invention provides a novel genetically modified Escherichia coli JM109 bearing accession number PTA 1579, containing the gene coding for poly-beta-hydroxybutyrate synthesis and a...
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6753145 |
Buffer composition and method for hybridization of microarrays on adsorbed polymer siliceous surfaces
A buffer composition, method and kit for hybridizing microarrays of nucleic acids bound to an adsorbed polymer surface of a siliceous substrate provide an envelope of conditions to hybridize...
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6750014 |
Reducing background in hybridization reactions
The invention provides a method for reducing background in hybridization reactions of nucleic acids involving at least two homologous probes, wherein at least one of the probes is non-linear, or...
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6750022 |
Mutation analysis by PCR and mass spectrometry
The invention concerns mass spectrometric analysis of known mutation sites in the genome, such as single nucleotide polymorphisms (SNPs). The invention uses minor amounts of primers with...
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6733989 |
Structural phosphoprotein (pp28) of human cytomegalovirus (HCMV), the preparation and use thereof
Structural phosphoprotein (pp28) of human cytomegalovirus (HCMV), the preparation and use thereof Expression of an SmaI fragment, which is about 1.0 kb in size, on the left-hand end of the HindIII...
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6730479 |
Detection of nucleic acid hybrids
Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined nucleic acid. Applications of those...
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6727066 |
Genes expressed in treated human C3A liver cell cultures
The present invention relates to a composition comprising a plurality of cDNAs which are differentially expressed in treated human C3A liver cell cultures and which may be used entirely or in part...
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6727068 |
Method for non-redundant library construction
Provided are novel methods for the production of nucleic acid libraries having reduced redundancy. Also provided are methods for determination of changes in RNA expression associated with...
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6723509 |
Method for 3′ end-labeling ribonucleic acids
Methods of end-labeling ribonucleic acids with non-radioactively labeled ribonucleotides, and particularly fluorescently labeled ribonucleotides, are provided. In the subject methods, a ribonucleic...
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6723510 |
Methods for separating particulate substrates from solution while minimizing particle loss
The present invention provides a method for separation of a particulate matrix from a solution while reducing loss of particles during separation steps. Methods are also disclosed for isolation of...
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6716602 |
Metabolic rate shifts in fermentations expressing recombinant proteins
The invention provides a method for increasing product yield of a polypeptide of interest produced by recombinant host cells, where expression of the polypeptide by the recombinant host cells is...
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6713073 |
Method of vaccination of newly hatched poultry
A method of vaccinating poultry by spraying the poultry with an effective amount of a live avirulent derivative of an enteropathogenic enterobacteria is disclosed.
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6709842 |
DNA encoding a growth factor specific for epithelial cells
Disccoveries are disclosed that show particular aspects of recombinant DNA technology can be used sucessfully to produce hitherto unknown human keratinocyte growth factor (KGF) protein free of...
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6706517 |
Method for producing L-amino acids by fermentation
The present invention provides an industrially efficient method for producing an L-amino acid useful as medicament, chemical agent, food material and feed additive, and the method comprising...
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6699685 |
Method, test media and chromogenic compounds for identifying and differentiating general coliforms and escherichia coli bacteria
A chromogenic β-galactosidase substrate producing an insoluble precipitate of a first color when reacted upon by β-galactosidase and a chromogenic β-glucuronidase substrate producing an...
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6692945 |
Process for the production of polyhydroxyoctanoate by streptomyces lividans
The present invention relates to a process for the production of polyhydroxyoctanoate, said method involving construction of a multifunctional Escherichia coli —Streptomyces conjugative shuttle...
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6682916 |
Chlorohydroxyacetone derivative and process for producing optically active chloropropanediol derivative from the same
A process for efficiently preparing an optically active chloropropanediol derivative, especially (S)-1-benzyloxy-3-chloro-2-propanol, which has a high optical purity and is useful as an...
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6677139 |
Methods for production of proteins in host cells
The present invention provides methods for the production of proteins, particularly toxic proteins, in host cells. The invention provides methods which use a fusion protein comprising a chaperonin...
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6664078 |
Method for the isolation of ccc plasmid DNA
The present invention relates to the production of biomass for the isolation of ccc plasmid DNA comprising culturing a bacterial transformant in a bioreactor containing an antibiotic-free batch...
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6660516 |
Method for culturing a microorganism and promoting microbial growth and metabolism
A method is provided for culturing a microorganism in a culture medium containing a carbon source and an electrolyzed water containing not more than 0.4 ppm chlorine produced during electrolyzing...
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6653111 |
Bacterial strain of Escherichia coli BKIIM B-3996 as the producer of L-threonine
The present invention provides a method for deriving an E. coli strain which produces L-theronine from E.coli strain VNIIgenetika 472T23 by transducing with bacteriophage P1 which bears a...
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6653112 |
Method for producing L-carnitine from crotonobetaine using a two stage continuous cell-recycle reactor
L(−)-carnitine is synthesized from crotonobetaine, crotonobetaine salts or derivatives in an ecologically advantageous manner by immobilizing cells of Escherichia coli 044 K74 on ceramics,...
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6649398 |
Method for inducing bacterial growth using autoinducers
A bacterial autoinducer and method for isolating and purifying a bacterial autoinducer form a sample comprising the steps of collecting a sample containing the autoinducer, fractionating the sample...
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6642203 |
Crustacean antimicrobial peptides
The invention concerns antimicrobial peptides obtained from penaeid prawns having the following characteristics: a molecular mass of about 5 to 7 kDa; a pHi not less than 9; an N-terminal portion...
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6617149 |
Method for isolation and identification of Escherichia coli 0157:H7 and plating media for said process
An isolation plating medium for use in processes for the presumptive identification of Escherichia coli 0157:H7 from a sample that also contains other strains of Escherichia coli. The plating...
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6605706 |
Method for producing a correctly folded, biological active recombinant protein
Methods for producing correctly folded biological active recombinant protein or polypeptide comprise the steps of expressing the protein or polypeptide in prokaryotic cells, harvesting the cells,...
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6589771 |
Methods for arousing dormant bacteria
A method for arousing dormant bacteria. The method comprises inducing diffusion of intracellular solutes from dormant bacteria and then allowing an adjustment period for a length of time sufficient...
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6586210 |
Polynucleotides encoding T1 receptor like ligand II
The present invention concerns a novel T1R-like ligand II protein. In particular, isolated nucleic acid molecules are provided encoding the T1R-like ligand II protein. T1R-like ligand II...
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6573083 |
Method of weight reduction in human beings
A method of weight reduction in human beings, wherein a human ingests a laxative effective dosage of non-pathogenic Escherichia coli to produce a weight loss of up to two pounds within one week.
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6569635 |
Growth state-specific immunofluorescent probes for determining physiological state and method of use
A novel method for examining bacterial growth state is carried out by measuring the levels of conserved cytosolic proteins specific for alternative growth states, using bacterial specific antibody...
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6552180 |
Isolated nucleic acid molecules coding for tumor rejection antigen precursor MAGE-2 and uses thereof
Isolated nucleic acid molecules are described, which have complements that hybridize to a nucleic acid molecule referred to as MAGE-2. The isolated nucleic acid molecules are expressed in some...
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6506590 |
Plasmids, their construction and their use in the manufacture of interleukin-4 and interleukin-4 muteins
The present invention relates to the construction and use of expression plasmids in the manufacture of recombinant interleukin-4 (IL-4) and interleukin-4 muteins.
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6495334 |
Recombinant Sef14 fimbrial protein from Salmonella
A truncated SE fimbria antigen useful as an antigen for immunoassay diagnosis of Salmonella enteritidis (SE) infection or evidence of infection.
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6472183 |
Immunity against Actinobacillus pleuropneumoniae's RTX toxins APX
Disclosed herein is a modified microorganism which produces an RTX toxin, wherein said RTX toxin is partially or fully inactivated. Also disclosed is a modified microorganism wherein an RTX toxin...
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6471966 |
Type 1 and type p fimbriae-adhesins isolated from novel e. coli strains, process for their preparation and uses thereof
Fimbriae adhesins have a molecular weight of 2×10 5 and 2×10 7 Da, and are comprised of 90-95% protein and 1-3% sugar. Type 1 fimbriae include five different protein fractions of 14-20 kDa,...
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6461815 |
Antibacterial agents and methods of screening for the same
Methods of inhibiting microbial propagation, and screening for compounds that inhibit microbial propagation, are described. A method of inhibiting microbial propagation comprises inhibiting...
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6451311 |
Anti-procalcitonin antibodies and the preparation and use thereof
The invention relates to anti-procalcitonin antibodies, their preparation and use, in particular in therapy and diagnostics. The antibodies comprise binding to procalcitonin but not to free...
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6432677 |
Non-human animal interferons
Distinct α-, β- and γ-interferon genes from various animal species have been identified, cloned and expressed to produce the corresponding non-human animal interferon proteins. Specifically...
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6432691 |
High-yield dual-buffered bacterial growth medium
A novel growth medium called “H15” is disclosed that supports much higher E. coli cell densities and, concomitantly, a much higher yield of plasmid than previously reported for small-scale...
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6410703 |
Identification of a vaccine candidate from an extraintestinal isolate of E. coli
The present invention is directed to identifying a gene, iroN ec , from an extraintestinal isolate of E. coli , CP9. This gene was identified from CP9 by screening a library of 527 mutant...
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6399074 |
Live attenuated salmonella vaccines to control avian pathogens
A vaccine for protecting birds against infection by avian pathogenic gram negative microbes is disclosed. The vaccine is a recombinant Salmonella strain expressing O-antigen of an avian pathogenic...
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6383496 |
Recombinant vaccines comprising immunogenic attenuated bacteria having RPOS positive phenotype
Attenuated immunogenic bacteria having an RpoS + phenotype, in particular, Salmonella enterica serotype Typhi having an RpoS + phenotype and methods therefor are disclosed. The Salmonella have...
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