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5206135 |
Use of the oxygenase activity of acetolactate synthase for herbicide detection
A process for the detection of herbicides that inhibit acetolactate synthase (EC 4.1.3.18) by use of the enzyme, a suitable substrate (pyruvate, α-aceto-lactate, α-ketobutyrate, or...
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5206148 |
Method for assaying aliphatic alcohol, aliphatic aldehyde or ω-carboxylic acid derivatives thereof
A method for assaying aliphatic alcohol, aliphatic aldehyde or ω-carboxylic acid derivatives thereof, using a novel ω-carboxyalcohol oxidase which catalyzes at least one of the following...
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5200325 |
Self-indicating analysis employing stoichiometric chemical subtraction
An analytical method and test composition for determining whether an analyte is present in a test sample at a predetermined concentration level by measuring or observing a preselected level of...
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5198336 |
Bioassay for chemicals which generate prooxidant states
A bioassay making use of submitochondrial particles to test for the presence of toxic substances which induce prooxidant states in vivo. The assay uses complex I of the electron transport enzymes...
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5196314 |
Process and reagent for the determination of substrates or enzyme activities
A process for the determination of substrate or enzyme activities by the use of a redox reaction as a measurement reaction is carried out in the presence of one or more additionally added...
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5196524 |
Fusion reporter gene for bacterial luciferase
Novel fusion reporter genes, fusion reporter proteins, and an improved reporter system for measuring the relative activity of a promoter sequence. A luxAB fusion gene of the present invention is...
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5196312 |
Method and test composition for determination of enzyme activity
Disclosed is a method for determination of an activity of γ-glutamyl transpeptidase, leucine aminopeptidase, alanine aminopeptidase, cystine aminopeptidase, X factor as a coagulation factor,...
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5190863 |
Composition for determining the presence or concentration of D-β-hydroxybutyrate
A composition, test device and method of determining the presence or concentration of ketone bodies, and specifically D-β-hydroxybutyrate, in a test sample are disclosed. The test device includes...
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5190872 |
Immobilized alcohol utidase for use in an alcohol measuring apparatus
Alcohol oxidase is immobilized by bonding an aminosilane coupling agent to a carrier, bonding a multifunctional aldehyde such as glutaraldehyde to an amino group of the aminosilane, washing the...
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5188941 |
Enzymatic determination of theophylline
The present invention provides a new methodology and test composition for determining the presence of theophylline in test samples. The methodology employs enzymes that utilize or recognize...
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5186842 |
Method for removing low molecular weight contaminants
A method of the present invention is provided for removing low molecular weight contaminants from a contaminant-containing material. This contaminant-containing material can include alcohol...
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5185247 |
Stabilization of oxidase enzyme-based test strips
A method and composition for the enzymatic assay of a liquid test sample, such as urine or serum, for a specific analyte, such as glucose. The method utilizes a dry phase test strip comprising a...
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5185249 |
Dry analytical element for assaying salicylate
A dry multilayer element for assaying salicylate is disclosed.
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5183742 |
Test device for detecting glucose, protein urobilinogen, and/or occult blood in body fluids and/or determining the PH thereof
A test device having the following meritorious effects is obtained in accordance with the present invention by using at least one composition selected from the group consisting of ink compositions...
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5183741 |
Integral multilayer element for glucose analysis
An integral multilayer element for chemical analysis utilizing an oxidase enzyme reaction system is prepared having in the following order: (a) a porous spreading layer; (b) an...
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5179002 |
Apparatus for determining whether respiratory gas is present in a gaseous sample
A method and apparatus are is disclosed for determining whether respiratory gas is present in a gaseous sample. The sample is brought into contact with an indicator which yields an indication...
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5175088 |
Rapid analysis of plural components
A new method of rapidly analyzing plural substances in the presence of biological catalyzers is disclosed. The method is practiced by way of the steps of injecting both pH buffer solution and...
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5171681 |
Omega-carboxyalcohol oxidase
A novel ω-carboxyalcohol oxidase catalyzes at least one of the following reactions: a) R--CH 2 OH+O 2 ➝R--CHO+H 2 O 2 b) R--CHO+O 2 +H 2 O➝R--COOH+H 2 O 2 wherein R is alkyl,...
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5171664 |
Monoclonal antibody to okadaic acids, process for producing the monoclonal antibody, assay reagent for assaying okadaic acids using the monoclonal antibody, and assay method
A monoclonal antibody which specifically binds okadaic acids and a process for producing the monoclonal antibody by culturing a cell strain capable of producing the antibody. The monoclonal...
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5171668 |
Method of the chemiluminescence assay of the activity of peroxidase
A method of chemiluminescence assay of the activity of peroxidase is disclosed, which employes (i) luminol or isoluminol as a substrate, (ii) hydrogen peroxide, and (iii) at lease one compound,...
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5169758 |
Stabilized, NAD(P)H-dependent, soluble nitrate reductase, a process for the preparation thereof and a reagent containing it
The present invention provides a stabilized, NAD(P)H-dependent, soluble nitrate reductase of the assimilatory type, characterized by a molecular weight of about 90,000 D in the case of...
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5168046 |
Method for determination of glucose concentration
There is provided a method for the determination of a glucose concentration in a whole blood utilizing a biosensor. A correction of the measured glucose concentration for dilution error introduced...
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5166049 |
Measurement of diaphorase activity and reagent therefor
A method for measuring diaphorase activity comprising mixing a sample comprising diaphorase with nitro blue tetrazolium, EDTA or a salt thereof, at least one of nicotinamide adenine dinucleotide...
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5162201 |
Analytical method making use of monoglyceride lipase
Disclosed herein is a novel monoglygeride lipase at least capable of catalyzing an enzymatic reaction of the following equation (a) and as substrate specificity, capable of acting on monoglyceride...
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5162202 |
Rectal mucus test and kit for detecting cancerous and precancerous conditions
An improved rectal mucus screening test method and kit for cancerous and precancerous conditions which is rapid, employs reagents which can be provided in kit form and which does not give false...
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5162203 |
Methods of measuring isozymes and isozyme classes of alcohol dehydrogenase
The present invention provides fluorescence-based methods for sensitively detecting total ADH activity in human sera and selectively measuring the activity of different classes of ADH in human sera...
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5158868 |
Method of sample analysis
A system for analyzing a biological fluid or the like for a constituent of interest comprises structure defining a sample inlet port, structure defining an analysis region, a measuring system...
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5156954 |
Assay device and method using a signal-modulating compound
An assay device and method for determining the concentration of an analyte. Analyte introduced by liquid absorption into the matrix is used to generate H2O2, via an analyte-specific oxidase, and...
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5156955 |
Nucleoside oxidase and assay method utilizing same
The present invention provides a novel nucleoside oxidase YT-1 serving as an enzyme for catalyzing oxidation reaction of a nucleoside, and oxidase being characterized in that the oxidase causes the...
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5155025 |
Hydrogen peroxide stabilization in assays
Hydrogen peroxide stabilized in the presence of blood components by the addition of a metal oxide oxidant, an anionic chelating agent, particularly hydroxylic carboxylate and nitroprusside, and a...
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5155024 |
Binder composition and analytical element having stabilized peroxidase in layer containing the composition
An analytical element has a peroxidase-labeled ligand analog distributed within a water-soluble binder composition comprising at least about 50 percent, by weight, of poly(vinyl alcohol). As a...
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5149633 |
Process and reagent for the specific determination of fructosamine content in blood and samples obtained from blood
Process for the specific determination of the serum fructosamine content in blood or in samples obtained from blood by reaction with an appropriate color reagent and measurement of the color change...
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5149630 |
Methods of assay
An electrochemical specific binding assay of a ligand (e.g., antigen, hapten or antibody) wherein at least one of the components is enzyme-labelled, and which includes the step of determining the...
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5141855 |
Signal amplifying cobalt (III) redox reagents and methods for the determination of analytes in aqueous fluids
Reagents and methods for the determination of analytes are disclosed, using water soluble cobalt(III) complexes and metallizable dyes to form cobalt(III) complexes of the metallizable dyes....
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5141853 |
Determination of ammonia levels in a sample
A method and kit for determining the amount of ammonia in a body fluid. The method and kit involve contacting the fluid with glutamate dehydrogenase, alpha-ketoglutarate and nicotinamide...
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5137807 |
Method for determining beta-subunit of human prolyl 4-hydroxylase by immunoassay to detect hepatic disease
The present invention is directed to a method for detecting hepatic diseases which are associated with fibrosis by determining the level of human prolyl hydroxylase in a serum sample which...
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5130233 |
Method for determining the β-subunit of human prolyl hydroxylase by radioimmunoassay to detect hepatic disease
A method for determining human prolyl hydroxylase by radioimmunoassay according to the sandwich method wherein a monoclonal antibody to human prolyl hydroxylase and polyclonal antibody to human...
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5126246 |
Reagent for analysis of triglycerides and analysis using the same
Reagent for analysis of triglycerides contained in blood serum is provided, which comprises lipases and monoglyceride lipases capable of acting on monoglycerides having substrate specificity and...
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5126275 |
Analytical method using tetrazolium salt indicators having a reflectance plateau
General classes of 2-thiazolyl tetrazolium salt compounds have been found to be characterized by a reflectance spectrum exhibiting an extended plateau above about 600-650 nm. Such compounds are...
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5126247 |
Method, system and devices for the assay and detection of biochemical molecules
Oxygen-independent methods, systems and devices for the enzymatic colorimetric assay and detection of biochemical analytes. Two systems are described, both of which produce less than one equivalent...
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5124254 |
Detection of diamines in biological fluids
A fast-acting and portable diagnostic kit for detecting diamines in biological fluids is in the form of a diamine oxidase and a chromogenic system to detect the presence of hydrogen peroxide...
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5122456 |
Amperometric method for the quantitative determination of 1,4-dihydronicotinamide adenine dinucleotide (NADH) in solution
A method is disclosed for the quantitative determination of 1,4-dihydronicotinamide adenisne dinucleotide (NADH) in solution. The method comprises contacting the NADH-containing solution with an...
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5116728 |
Reagents for CO.sub.2 detection
A stabilizer for maintaining a constant level of CO 2 in coenzyme containing enzymatic CO 2 diagnostic reagents is disclosed, comprising rate-limiting amounts of (a) the diagnostic reagents and...
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5116730 |
Amino acid substituted 4-aminophenazones for measuring enzyme activity
Novel substrates which are amino acid 4-amino phenazones for detecting enzymes, particularly L-gamma-glutamyltransferase, are described. The amino acid gamma-glutamyl-4-aminophenazone reacts with...
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5116732 |
Tetrazolium halide compounds and methods
Compounds of the formula DNB-TZ(P2) (P3) X, wherein TZ is a tetrazolium ring, DNB is 5-(2,4-dinitrophenyl), X is halide and P2 and P3 are each independently halophenyl, nitrophenyl or phenyl. The...
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5116725 |
Assay for Epstein-Barr virus infection with solid phase bound synthetic polypeptides
Antigens, immunogens, inocula, antibodies, and particularly diagnostic methods and systems relating to Epstein-Barr virus nuclear antigen (EBNA) are disclosed. The diagnostic methods and systems...
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5114350 |
Controlled-volume assay apparatus
Method and apparatus for use in determining the concentration of a selected analyte in a body-fluid sample. As the fluid sample is applied to a wettable, absorbent reaction pad, the extent of...
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5112741 |
Acetaldehyde trapping system
The invention provides a reagent system for the enzymatic determination of an oxidizable substrate in a fluid sample; the system includes an active amine trap for inactivating high concentrations...
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5110724 |
Multi-analyte assay device
An assay device for assaying multiple analytes in a drop-size blood sample. The device includes a sample dispenser designed to distribute a small-volume blood sample to multiple transfer sites, by...
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5108903 |
Reducible compounds which provide leuco dyes for analytical compositions, elements and methods of using same
Certain reducible compounds are useful in analytical compositions, elements and methods, for example for the detection of bacterial cells. These compounds comprise a moiety which provides a leuco...
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