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5474907 |
Multilayer analytical element for salicylate assay
A multilayer analytical element for measuring salicylate is disclosed. The element has a support. The support is coated with the following layers, top down to the support: a) a spreading layer, b)...
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5472862 |
Sorbitol oxidase from Xanthomonas maltophilia FERM BP-4512
An isolated sorbitol oxidase from Xanthomonas maltophilia FERM BP-4512 is disclosed. The oxidase catalyzes the reaction of D-sorbitol+O 2 ➝D-glucose+H 2 O 2 , has a substrate specificity for...
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5462858 |
Dry multilayer analytical elements for assaying transaminases
A dry multilayer analytical element for assaying transaminases wherein the analytical element comprises: a) a transaminase substrate; b) reagents that generate pyruvate in the presence of a...
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5460948 |
Assay of salicylates or reduced pyridine nucleotides
A method for the estimation of a salicylate in a sample is characterized by enzymatically converting the salicylate to a catechol by the action of a salicylate mono-oxygenase enzyme on the...
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5459032 |
Method of conducting a non-instrumental test to determine catalyst presence
A method and apparatus for performing an enzyme spot test without traditional instrumentation is disclosed. The method utilizes various reagents to allow the visual determination of the presence of...
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5453358 |
Method of measuring cholesterol diagnosing vascular disease
A method is provided for measuring the net HDL cholesterol in the blood. The LDL and VLDL moieties are precipitated out and the remaining cholesterol content of the supernatant is measured. The...
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5453360 |
Oxidative coupling dye for spectrophotometric quantitive analysis of analytes
A dye couple, comprising 3-methyl-2-benzothiazolinone hydrazone (MBTH) and 8-anilino-l-naphthalenesulfonate (ANS), is used as an indicator in a reaction cascade producing a strong oxidizing agent,...
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5447847 |
Quantitative determination of pyruvic acid and quantitative analysis for component of living body making use of such determination
Disclosed herein is a quantitative determination of pyruvic acid, which comprises reacting oxidized nicotinamide adenine dinucleotide, a pyruvate dehydrogenase complex and coenzyme A to a specimen...
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5445943 |
Method for the colorimetric determination of an analyte by means of benzyl alcohol dehydrogenase and a chromogenic redox indicator
The invention concerns a method for the colorimetric determination of NADH by means of enzymatic oxidation with benzyl alcohol dehydrogenase whereby an aromatic nitrosoaniline compound of the...
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5445944 |
Methods for determining peroxidately active substances
Methods and compositions are disclosed for determining a peroxidatively active substance (PAS). The methods comprise the step of detecting a fluorescent signal produced upon cleavage of a compound...
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5445946 |
Intravacuolar stains for yeast and other fungi
The invention relates to a method of forming distinctive intravacuolar structures that are unique to fungal cells and can be correlated with cell viability. The preferred dyes of the present...
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5443986 |
Enzymatic analysis using substrates that yield fluorescent precipitates
Activity of enzymes and enzyme conjugates is detected using novel substrates made from a class of fluorophores, generally including quinazolinones (quinazolones), benzimidazoles, benzothiazoles,...
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5443961 |
Method for diagnosing cellular aging or inflammation condition of keratinocytes, and kit for implementing such method
Method for diagnosing a cellular aging or inflammation condition of keratinocytes of the skin or a pilous follicle in a person, or for diagnosing the efficiency of a treatment intended to combat...
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5441872 |
Method for the analysis of Vitamin C
A new enzymatic rate method is disclosed for the analysis of Vitamin C using ascorbate oxidase. Total Vitamin C can be measured in a single analysis, in a procedure amenable to automation. Any...
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5434055 |
Compositions useful in anaerobic determination of analytes
The invention describes a composition useful in determining sample analytes, where the determination is carried out anaerobically. The compositions include an analyte oxidizing agent, an electron...
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5426033 |
Method for enzymatic determination of substrate after enzymatically removing galactose
A method for quantitating accurately 1,5-anhydroglucitol, fructose or amylase in a sample that contains galactose using an enzymatic determination involves a pretreatment which removes galactose...
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5422246 |
Electrode having a polymer coating with a redox enzyme bound thereto, the polymer coating being formed on the walls of pores extending through a porous membrane
An electrode is composed of a membrane, provided with open pores running through said membrane, the walls of the pores having an electrically conducting polymer coating, containing a redox enzyme...
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5418142 |
Glucose test strip for whole blood
A separation matrix impregnated with a separating agent separates red blood cells or hemoglobin from whole blood. Upon separation, an impregnated test reagent reacts with the separated...
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5416004 |
Multilayer analytical element containing primary amine buffer and method for the determination of ethanol
A multilayer analytical element has been prepared for accurate and rapid colorimetric determination of ethanol in aqueous specimens using alcohol dehydogenase and an oxidized nicotinamide coenzyme....
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5413915 |
Method and sensor for detecting toxic chemical exposure effects and metabolic activation of carcinogenic chemical agents
A metabolically active biological component having mixed function oxidation activity. e.g., microsomal P-450 oxidase enzyme complex, is effective in the presence of NADPH and moisture when...
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5413916 |
Colorimetric toxicity test
A method is described for determining the toxicity of an environmental sample to bacteria. The method includes the use of resazurin dye as an indicator. Glutaraldehyde is used as an accelerating...
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5411870 |
Process and apparatus for direct determination of low density lipoprotein
Low density lipoproteins are directly determined in fluid samples by selectively precipitating low density lipoproteins from the sample by forming clusters, selectively consuming the high density...
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5411866 |
Method and system for determining bioactive substances
An apparatus and method for measuring glutamine and glucose in certain cell cultures and body fluids in the presence of interfering endogenous compounds e.g. ascorbic acid, uric acid or glutamic...
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5409814 |
Determination of ions in fluids
A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium,...
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5407818 |
Biosensor containing a biochemical substance immobilized on a layer of olefinic-unsaturated, epoxy functional cross-linked polysiloxane
A biosensor is prepared having a selective detection system containing a biochemical substance such as an enzyme immobilized on an olefinic-unsaturated, epoxyfunctional polysiloxane. Prior to...
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5407808 |
Method and composition for photodynamic treatment and detection of tumors
Disclosed is a method and a pharmaceutical composition for killing mammalian tumor cells by subjecting the cells to light in the presence of a light-activatable tetrapyrrole, in which the...
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5405773 |
Assay for motile facultative anaerobic pathogens
An approved assay for the determination for motile facultative anaerobic pathogens is described which involves the use of a growth-enhancing substance such as oxyrase enzyme in order to increase...
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5403450 |
Method of water purification
A method for converting reducible or oxidizable substances from aqueous solution is claimed in which the aqueous solution to be treated is brought into contact with immobilized oxidoreductases, if...
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5391483 |
Ligand analogs for immunoassays derived from dicarboxylic acid oxidation products
What is disclosed is a ligand analog comprising a dicarboxylic acid oxidation product of a immunologically reactive mono-or a polysaccharide having vicinal diols, to which a label or a support is...
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5389522 |
Serum antioxidants as predictors of the adult respiratory distress syndrome in septic patients
At the initial diagnosis of sepsis (6-24 h before the development of ARDS), serum manganese superoxide dismutase (MnSOD) levels and catalase activities are increased in septic patients who...
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5385828 |
Method for determining the relative amounts of all cholesterol-containing lipoproteins in body fluids
The invention refers to a method for determining the relative amounts of all cholesterol-containing lipoproteins in body fluids comprising electrophoretically separating the lipoproteins of an...
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5384246 |
Determination of ions in fluids by a process involving displacement of indicator ions
A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium,...
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5384248 |
Process for measuring an analyte with an oxidase and an oxidizable color reagent and surfactants
A substrate or an enzymatic activity in a body fluid can be measured accurately without influences of interfering substances such as bilirubin by reacting an oxidase corresponding to an analyte...
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5384247 |
Determination of sodium ions in fluids
A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium,...
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5382523 |
Use of sparingly soluble salt of a heteropoly acid for the determination of an analyte, a corresponding method of determination as well as a suitable agent thereof
A sparingly soluble salt of a heteropoly acid is used for the determination of an analyte which is an aromatic amine rich in electrons or together with another substance leads to such an amine. The...
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5382516 |
Method and devices for delivery of substrate for the detection of enzyme-linked, membrane-based binding assays
The invention provides a method for supplying an enzyme substrate to a membrane-based, enzyme-linked reaction, comprising providing an open pore, high liquid retention capacity material impregnated...
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5380649 |
Enzymatic determination of analyte ions in fluids by optimizing measurement levels
A process and a reagent for the determination of ions in fluids, wherein the influence of these ions on the activity of an enzyme is measured. The ions for example are sodium, potassium, calcium,...
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5374534 |
Method of preparing D-luciferin derivatives
A method of preparing a 6-substituted D-luciferin ester suitable for use in the determination of pesticides by a bioluminescence method. The 6-substituted D-luciferin esters, such as 6-D-luciferin...
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5370990 |
Diagnostic assay for fructosamines
A method for the determination of glycated protein in a sample characterised in that it comprises treating the sample with a protease and treating the protease-treated sample with a ketoamine...
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5364767 |
Chromogenic compounds and methods of using same
The present invention relates to chromogenic compounds which are represented by the general Formula (I): ##STR1## wherein R 1 is a sugar group, ester group, hydrocarbyl group, phosphate group,...
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5362630 |
Isolation of pseudomonas salicylate hydroxlase and its use for the identification and quantitation of salicylate in body fluids
Salicylate hydroxylase isolated from Pseudomonas bacteria can be used to determine the level of salicylate in a body fluid by reacting a sample of the fluid with the enzyme and monitoring the...
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5362628 |
Fluorescent haloalkyl derivatives of reporter molecules well retained in cells
The subject invention provides a method for analyzing the metabolic activity in cells by improving the retention of a detectable reporter molecule only in intact cells where a particular enzyme is...
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5362633 |
Method of assaying for peroxidatively active substances
A method of determining the presence or concentration of a peroxidatively active substance, such as hemoglobin, in a test sample is disclosed. The method comprises contacting the test sample with...
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5356780 |
Determination of substrate of coenzyme requiring enzyme
The invention relates to a competitive binding assay technique for substances (e.g. small molecules having no antigenic determinant) which can serve as substrates for coenzyme-requiring enzymes....
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5356790 |
Highly sensitive assay method for myo-inositol, composition for practicing same, novel myo-inositol dehydrogenase, and process for producing same
Myo-inositol in a specimen is assayed by reacting a specimen containing myo-inositol with: a) myo-inositol dehydrogenase using a thio-NADP group or thio-NAD group and an NADP group or NAD group...
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5352574 |
Surface functionalized and derivatized electroactive polymers with immobilized active moieties
New variants of electroactive and optoactive polymers are formed from the surface chemical modification and derivization of free-standing and substrate-supported polymer films. The free-standing or...
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5348860 |
Screening test and kit for cancerous and precancerous conditions
An improved screening test method and kit for cancerous and precancerous conditions which is rapid, employs reagents which can be provided in kit form and which does not give false negatives due to...
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5344753 |
Dry analytical element and method for the detection of an aminopeptidase or transpeptidase
A dry analytical element has been prepared for the assay of leucine aminopeptidase at a pH of 6.5 to 11. The element zone contains an aromatic substrate for the enzyme. This substrate provides an...
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5340722 |
Method for the determination of the concentration of an enzyme substrate and a sensor for carrying out the method
A method for the continuous and reversible determination of the concentration of an enzyme substrate such as glucose in an specimen, wherein the specimen is brought into contact with a...
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5334502 |
Method of collecting, identifying, and quantifying saliva
A method is provided for rapidly determining during a saliva specimen collection procedure the presence of an amount of saliva, and for verifying that the sample obtained is in fact saliva. Color...
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