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9017962 Intracellular reporters  
The invention provides a variety of molecular tools for use in live-cell tracking of activities of biomolecules and in high-throughput drug screening.
9012145 Protein tyrosine phosphatase mutations in cancers  
Tyrosine phosphorylation, regulated by protein tyrosine phosphatases (PTPs) and kinases (PTKs), is important in signaling pathways underlying tumorigenesis. A mutational analysis of the tyrosine...
8980544 Boronic and borinic acid compounds as inhibitors of sulfenic acid-containing proteins  
A boronic or borinic acid compound is used to inhibit the activity of a sulfenic acid-containing protein. Thus, a biologically-active sulfenic acid-containing protein is contacted with an...
8956824 Methods for identifying allosteric modulators of ship polypeptides  
This invention provides a method for identifying allosteric modulators of a SHIP polypeptide, wherein said SHIP polypeptide is contacted with a test compound, and wherein said SHIP polypeptide...
8951748 Rapid detection of molds that produce glucose oxidase  
Methods and kits are disclosed for detecting microorganisms that produce glucose oxidase. The method includes providing a culture medium and a hydrogen peroxide indicating reagent comprising a...
8865420 Methods and compositions for risk stratification  
The present invention provides an approach for the simultaneous determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of...
8859220 Luciferase-based assays  
A method and kit are provided for enhancing the tolerance of an assay reagent to compounds in an assay sample, the assay reagent including a luciferase enzyme. The method includes contacting the...
8802389 Identification of toxin-binding protein involved in resistance to Cry1 toxins, and related screening methods  
The subject invention relates in part to the surprising and unexpected discovery that insects that are resistant to Bacillus thuringiensis Cry toxins have measurably altered alkaline phosphatase...
8790886 Methods to assay kinase activity  
Methods to assay kinase activity are provided herein. The methods employ elemental analysis, including inductively charged plasma mass spectrometry (ICP-MS). The methods allow for the convenient...
8748120 Method of selecting a surface chemistry for culturing a given cell line  
In one embodiment of the invention, there is provided a method of selecting a surface chemistry for culturing a given cell line, including the steps of: (i) providing a first substrate having a...
8748119 Methods for determining calcineurin activity, and uses in predicting therapeutic outcomes  
One aspect of the present disclosure encompasses methods for determining a protein kinase or phosphatase activity in a biological sample, comprising: contacting in a reaction mix a first test...
8741597 Reaction medium for methicillin-resistant Staphylococcus aureus  
A reaction medium for detecting and/or identifying Staphylococcus aureus bacteria, comprising a combination of two enzymatic substrates for alpha-glucosidase.
8735087 Use of alkaline phosphatase for preventing or reducing liver disease  
The present invention provides a use for alkaline phosphatase for the manufacture of a medicament for the prevention or reduction of toxic LPS influx through a mucosal lining of a mammalian body...
8703437 Fluorogenic sensors for phospholipase C isozymes  
The present invention provides fluorogenic substrates and methods of use in detecting and analyzing phospholipase C isozyme (PLC) activity.
8697381 Methods for identifying modulators of RGS21 activity, compositions comprising an RGS21 Modulator, and methods of use thereof to modulate taste sensation  
The present invention provides methods for identifying compounds that selectively and specifically modulate RGS21 gene expression, RGS21 protein expression, and/or the interaction of RGS21 with G...
8669074 Chimeric phosphorylation indicator  
A chimeric phosphorylation indicator (CPI) as provided herein can contain a donor molecule, a phosphorylatable domain, a phosphoaminoacid binding domain (PAABD), and an acceptor molecule. Where...
8642283 Screening assay for agents that alter target of rapamycin activity  
The present invention provides an assay for the identification of agents which can modulate TOR-mediated phosphorylation of substrate proteins. The assays of the invention utilize substrate...
8603765 Immunoassay reagents and methods of use thereof  
The present invention provide reagents and methods of using the reagents, for example, on automated staining devices, that facilitate detection of two or more antigens in a sample simply and...
8574864 Methods and kits for 3'-end-tagging of RNA  
The present innovation provides methods and kits that enable rapid and efficient dual end-tagging of RNA to prepare libraries for analysis by applications such as next-generation RNA sequencing,...
8574863 Alkaline phosphatase for treating an inflammatory disease of the gastro-intestinal tract  
The present invention provides a use for alkaline phosphatase for the manufacture of a medicament for the prevention or reduction of toxic LPS influx through a mucosal lining of a mammalian body...
8563263 Enhancement of vanadium-containing phosphatase inhibitors  
The present invention is directed to a composition comprising a vanadium-containing phosphatase inhibitor and a polyol. In the presence of the polyol the effect of the inhibitor is enhanced, even...
8546095 Methods for determination of calcineurin activity and uses in predicting therapeutic outcomes  
The methods of the disclosure provide fluorescence-based assays for calcineurin activity, especially in isolated T cells. The methods include the stimulation of the T cells with agents that...
8530179 Electrochemical methods of detecting cancer with 4-aminophenyl phosphate  
A method of detecting cancer cells is disclosed. The method comprises (a) contacting the cell which is suspicious of a cancerous or undifferentiated phenotype with 4-aminophenyl phosphate (p-APP)...
8530178 Hydrolase detection system with caged substrates  
An enzyme detection method includes forming a caged substrate; releasing an uncaged substrate by cleaving a caging molecule from the caged substrate; and emitting a light emission from a...
8501428 Single molecule detection platform, manufacturing method thereof and method using the same  
A single molecule detection platform is disclosed. The single molecule detection platform comprises a light-transmissive substrate, a plurality of spherical particles and a thin film. The surface...
8497086 Reaction medium for methicillin-resistant Staphylococcus aureus (MRSA) bacteria  
A reaction medium for detecting and/or identifying methicillin-resistant Staphylococcus aureus (MRSA) bacteria includes a chromogenic substrate, a first antibiotic that belongs to the...
8486651 Method for treating a disorder associated with Eya dysregulation by modulating Eya phosphatase activity  
The present invention provides a novel mechanistic pathway and methods related to this pathway for the identification of compounds for the treatment of diseases involving cell proliferation,...
8486652 Method for the in vitro diagnosis of stroke  
A method for the in vitro diagnosis of stroke and transient ischemic attack (TIA) in an individual, including the following steps: (a) measuring the level of proBNP(1-108), or of fragments of...
8470745 Methods and compositions for obtaining and using biologically active multi-protein complexes  
Methods for isolating and using multi-protein complexes that are biologically active are provided. The complexes contain one or more proteins of interest (e.g. a receptor, ion channel, etc.) and...
8460862 Enzyme substrates for visualizing acidic organelles  
The present invention relates to the visualization of acidic organelles based upon organelle enzyme activity. The organelle substrates of the invention are specific for enzyme activity of the...
8445412 Separation of pyrophosphate release and pyrophosphate detection  
The present technology relates to methods and systems for detection of pyrophosphate. As such, disclosed herein are methods and systems that permit improved pyrophosphate detection. Also disclosed...
8431356 Fluorescence resonance energy transfer assays for sarco/endoplasmic reticulum calcium atpase and phospholamban  
Provided herein are methods for identifying molecules capable of modulating SERCA, the SERCA-PLB complex or the microenvironment of the complex. An exemplary assay provided herein is fluorescence...
8420345 Method for identifying at least two groups of microorganisms  
The present invention relates to a method for identifying at least two groups of microorganisms expressing the same enzymatic activity, comprising the following steps: a) incubating said groups of...
8399213 Method for monitoring intracellular tyrosine phosphatase activity  
The invention relates in general to tyrosine dephosphorylation. More specifically, the invention relates to methods and compositions for monitoring intracellular tyrosine dephosphorylation at the...
8367363 Method of determining the probability of a therapeutic response in cancer chemotherapy with cardiac glycoside  
A prognostic assay and kit and method of use thereof are provided. The kit and assay are used to determine the likelihood of a diseased cell or tissue having a therapeutic response to treatment...
8357494 Anti-clusterin oligoclonal antibodies for diagnosis and prediction of the aggressiveness of tumours, diagnostic method and related kits  
The invention concerns anti-clusterin oligoclonal antibodies able to recognize and bind in a selective and specific way antigenic epitopes of clusterin isoforms to be used in tumors diagnosis and...
8338456 Cut-point in PTEN protein expression that accurately identifies tumors and is predictive of drug response to a pan-ErbB inhibitor  
A cut-point in the quantitative measurement of PTEN protein expression that accurately identifies tumors with two inactivated alleles of the PTEN gene. Patients with a normalized PTEN score of...
8338125 Kit for measuring nitric oxide synthase activity  
The present invention relates to kits for measuring nitric oxide synthase activity.
8338124 Methods for inducing differentiation of undifferentiated mammalian cells into osteoblasts  
The present invention relates to in vivo and in vitro methods, agents and compound screening assays for inducing differentiation of undifferentiated mammalian cells into osteoblasts, including...
8338126 Kit for detecting and measuring element tagged kinases and phosphatases by inductively coupled plasma mass spectrometry  
Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate...
8318449 Method of measuring the activity of lipid-modified enzyme  
It is intended to provide an assay method whereby the activity of a lipid-modifying enzyme can be conveniently measured over a wide range and a drug capable of controlling a lipid-modifying enzyme...
8288118 Method of analyzing various surface chemistries for culturing a given cell line  
In one embodiment of the invention, there is provided a method of analyzing various surface chemistries for culturing a particular cell line, which includes the steps of: (1) providing a plasma...
8278086 Enhancement of vanadium-containing phosphatase inhibitors  
The present invention is directed to a composition comprising a vanadium-containing phosphatase inhibitor and a polyol. In the presence of the polyol the effect of the inhibitor is enhanced, even...
8268577 Electrochemical methods of detecting colon cancer cells and use of same for diagnosing and monitoring treatment of the disease  
A method of detecting cancer cells is disclosed. The method comprises (a) contacting the cell with a substrate for an enzyme under conditions wherein the enzyme catalyzes a reaction of the cell...
8227221 Processes of producing fermentation products  
The invention relates to a process of fermenting plant material in a fermentation medium into a fermentation product using a fermenting organism, wherein increased levels of one or more...
8206941 Three part assay for kinase or phosphatase activity  
The present invention relates to a method of determining kinase or phosphatase activity based on a three parts system. The method comprises contacting a binding partner which can bind...
8183007 ADP detection based methods using adenylate cyclase and bioluminescence  
The disclosure provides compositions and methods to determine or detect ADP or the activity of enzymes, including phosphotransferases such as kinases (e.g., protein, lipid, and sugar kinases) and...
8153436 Assays for disease-associated crystals in biological samples  
Methods of assaying for disease-associated crystal species in biological samples are described. Such methods involve contacting a patient sample with an excess of a detectable crystal-tagging...
8137956 Forensic test strip and method for the detection of semen  
The present invention relates to a forensic test strip and method for the detection of semen. This strip is comprised of a paper element coated with reagents which react to the presence of acid...
8097431 Methods and compositions for detecting steroids  
The present invention provides for methods and systems for detecting steroids. Examples of such steroids include estrogen, progesterone, androgen, testosterone, and derivatives and analogs...