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4952495 Hydrolyzable compounds which release electron transfer agents and analytical use of same  
Hydrolyzable substrates comprise blocked moieties which, when cleaved from the substrate during hydrolysis, provide electron transfer agents. The released electron transfer agents can be recycled...
4948726 Enzyme immunoassay based on membrane separation of antigen-antibody complexes  
An enzyme immunoassay based on membrane separation of antigen-antibody complexes wherein human or animal body fluid specimens containing an antigen are mixed with an enzyme-conjugated antibody...
4946776 Stable chromogenic substrate mixture of indoxyl phosphate and tetrazolium salt, method of making and using same in biological and diagnostic assays  
A stable chromogenic substrate mixture of indoxyl phosphate and tetrazolium salt is prepared by dissolving the phosphate in DMF and combining the same with Tris buffer containing magnesium chloride...
4933278 Method of determining the number of cells in cell culture  
A method is disclosed for determining the number of cells in cell culture which comprises lysing the cells and incubating the cell lysate with p-nitrophenyl phosphate for a predetermined period of...
4921786 Novel NAD synthetase, assay method using said novel NAD synthetase and a process for production thereof  
A novel NAD synthetase is produced by culturing a broth of Bacillus stearothermophilus H-804 FERM BP-1408. This new enzyme selectively catalyzes the reaction ##STR1## without catalyzing the...
4914025 Export of intra-cellular substances  
An export sequence of export DNA can be identified by transforming a population of cells with a vector having a transposon that includes a structural gene encoding a detectable compound, positioned...
4914023 Method of extending the linear range of an enzyme immunoassay by measuring off-peak absorbance  
The present invention relates to enzyme immunoassays employing one or more enzyme label-substrate pairs which give rise to colored products and in which absorption by the product of substrate...
4908306 Human papillomavirus 56 nucleic acid hybridization probes and methods for employing the same  
Nucleic acid hybridization probes for human papillomavirus types and particularly human papillomavirus type 56; and methods for employing the same.
4904583 Cascade immunoassay by multiple binding reactions  
A method for enzyme immunoassay includes contacting under binding conditions a liquid suspected of containing an analyte, an antianalyte affixed to a solid support and a tracer having an enzyme...
4900665 Integral multilayer analytical element for use in the measurement of alkaline phosphatase activity  
An integral multilayer analytical element for use in the measurement of alkaline phosphatase activity, which comprises a porous spreading layer of woven fabric or knitted fabric containing an...
4892817 Stable phosphatase substrate composition  
A stabilized phosphatase substrate composition is provided comprising an indoxl phosphate, a tetrazolium salt, and a water-soluble ferricyanide/ferrocyanide salt. When present as a solution, the...
4889799 Reagent kit producing shortened target DNA segments usable in sequencing large DNA segments  
A process is provided for producing an ordered series of cloned, circular, DNA molecules containing shortened target DNA segments derived from a long target DNA segment, which are suitable for use...
4888288 Vesicles resistant to enzyme lysis and use thereof in an enzyme assay  
Liposomes are formed from compounds including a phosphonate or phosphinate group instead of a phosphate group. The liposomes bind to enzymes, such as a phospholipase, and such liposomes containing...
4886741 Use of volume exclusion agents for the enhancement of in situ hybridization  
This invention relates to methods for using volume exclusion agents to enhance in situ hybridization rates between short oligonucleotide probes and their target polynucleotides where the cells...
4882423 Substance-conjugated complement component C1q  
A substance-conjugated complement component Clq is provided. A substance such as signal emitting substances or cell function regulating substances is conjugated via a sulfur atom to at least one...
4879218 Antibody for C.difficile  
Monoclonal antibody to toxin A of C.difficile has been prepared and is used in an assay for toxin A of C.difficile.
4861709 Detection and/or identification of microorganisms in a test sample using bioluminescence or other exogenous genetically-introduced marker  
A method for determining the presence of microorganisms in a tests sample. Exogenous DNA containing a luminescent system or other genetic marker system derived from a suitable donor source is...
4855227 Rapid detection of mycoplasmas  
A clinical diagnostic method capable of rapidly detecting the presence of Mycoplasma pneumoniae in infected humans is taught. The method allows a proper course of therapy to be chosen within one...
4849332 Human papillomavirus 35 nucleic acid hybridization probes and methods for employing the same  
Nucleic acid hybridization probes for human papillomavirus types and particularly human papillomavirus type 35; and methods for employing the same.
4849337 Assaying allergen specific IgE levels with fluorogenic enzyme labeled antibody  
A method for identifying and quantifying allergen specific IgE levels in patent serum by conjugating the IgE in the serum with allergens adhering to an insoluble support, conjugating the serum IgE...
4849335 Method for detecting the mutagenicity of substances susceptible of inducing the deterioration of cellular DNA, utilizing the production of an SOS response  
A method for the detection of the mutagen effect of a substance or composition by producing culture of a micro-organism harboring a recombinant of a sfi gene and of a gene coding for a dosable...
4849331 Human papillomavirus 44 nucleic acid hybridization probes and methods for employing the same  
Nucleic acid hybridization probes for human papillomavirus types and particularly human papillomavirus type 44; and methods for employing the same.
4849334 Human papillomavirus 43 nucleic acid hybridization probes and methods for employing the same  
Nucleic acid hybridization probes for human papillomavirus types and particularly human papillomavirus type 43; and methods for employing the same.
4845028 Composition for lipase assay  
A composition and method for lipase assay, comprising the use of an aqueous solution of a 1,2-diglyceride of a higher fatty acid, and a nonionic surface active agent. The higher fatty acid is a...
4845027 Fluorometric assay of allergic reactions  
A method for identifying and quantifying allergen specific IgE levels in patient serum by conjugating the serum IgE with allergens on an insoluble support, conjugating the serum IgE with an enzyme...
4844966 Assaying total IgE levels with fluorogenic enzyme labeled antibody  
A method for identifying and quantifying total IgE levels in patient serum by conjugating the IgE in the serum with anti-IgE antibody adhering to an insoluble support, conjugating the serum IgE...
4840895 Monoclonal antibodies reactive with immune complexes  
A secondary monoclonal antibody against a complex of a molecule of molecular weight less than 5000 and a binding protein against said molecule which secondary monoclonal antibody is not an antibody...
4835099 Signal enhancement in immunoassay by modulation of enzymatic catalysis  
A method for enzyme immunoassay for a ligand suspected to be present in a liquid sample includes signal amplification by use of at least two hydrolases and a blocked fluoroketone for one of the...
4830960 Determination of chlamydia trachomatis  
A process for determining qualitatively or quantitatively the presence of Chlamydia Trachomatis or Chlamydia trachomatis derived material in a subject material which process comprises deriving a...
4830959 Electrochemical enzymic assay procedures  
Electrochemical detection of mediator level is employed in a method of assay using a redox enzyme and redox substrate to detect conversion to an effective mediator by an assay enzyme label of a...
4828982 Vesticles and use thereof in an enzyme assay  
A sac is produced by use of a compound which includes a hydrophobic portion and a polar group which is hydrolyzable by an enzyme and which is spaced from the hydrophobic portion by a hydrophilic...
4828985 Antibodies against the complex of a small molecule and its binding protein, their preparation and their use in diagnostic methods  
The invention involves polyclonal antibodies reactive with a complex of a hapten and its binding protein, wherein the antibodies are unreactive with the free hapten or free binding protein. The...
4820627 Method of preparing particles suitable for tabletting into diagnostic reagents  
A completely water-soluble, solid, labile biochemical-containing diagnostic reagent is prepared without the need of a lyophilization step, by spraying, preferably by a fluidized bed process, an...
4812400 Process for measuring sodium levels in biological fluids  
A process for measuring the sodium concentration of a biological fluid, such as blood serum or urine, is carried out by fixing the amounts of adenosine-5'-triphosphate (ATP), adenosine...
4812396 Method for detecting enzymatic activity using particle agglutination  
A method is disclosed for determining enzymatic activity in a liquid sample by particle agglutination or inhibition of particle agglutination.
4810636 Chromogenic acridinone enzyme substrates  
Chromogenic acridinone enzyme substrate compounds comprising 7-hydroxy-9H-acridin-2-one chromogens derivatized at the 7-hydroxy-position with an enzymatically-cleavable group and disubstituted at...
4810631 Signal enhancement in immunoassay by modulation of chemical catalysis  
A method for immunoassay for a ligand suspected to be present in a fluid includes use of an enzyme, a metal ion catalyst for an indicator reaction and a blocked modulator for the catalyst. Ligand...
4808522 Enzyme immunoassays using inorganic pyrophosphatase  
A method for determination of an antigen, which comprises preparation of a onjugate by way of cross-linking of an antibody with an inorganic pyrophosphatase, reacting the resulting conjugate with...
4806415 Method and system for determining the presence of adenosine triphosphate or flavin mononucleotide  
An enzymatic determination of one of the co-enzymes adenosine triphosphate (ATP) or flavin mono-nucleotide (FMN) based on the use of an flavine adenine dinucleotide (FAD) synthetase-active system...
4806470 Analytical element and method for theophylline determination having increased alkaline phosphatase isoenzyme  
Theophylline can be determined with an analytical element and method which utilize the inhibition, by theophylline, of alkaline phosphatase activity on an appropriate substrate. The assay is...
4803157 Hydrolyzable fluorescent substrates for phosphatases and analytical use thereof  
Hydrolyzable substrates for acid and alkaline phosphatases comprise blocked dye moieties which, when cleaved from the substrate during hydrolysis, provide fluorescent dyes having maximum...
4795701 Homogeneous polynucleotide displacement assay method kit and reagent complex  
An RNA signal strand is displaced from a probe strand by a target nucleotide sequence. Without separation, a digestion enzyme selective for displaced RNA, e.g., a polynucleotide phosphorylase,...
4788143 Method for determining levels of cell-mediated immunity  
A patient's level of cell-mediated immunity is determined by obtaining a blood sample from the patient, incubating cells from the sample in a tissue culture medium containing Concanavalin A, and...
4782017 Analytical element and method for determination of theophylline by enzyme inhibition  
Theophylline can be determined with an analytical element and method which utilize the inhibition, by theophylline, of alkaline phosphatase activity on an appropriate substrate. The assay is...
4782016 Analytical element and method for determination of theophylline by enzyme inhibition  
Theophylline can be determined with an analytical element, composition, kit and method which utilize the inhibition, by theophylline, of alkaline phosphatase activity on an appropriate substrate....
4778755 Immunoassay method  
In the determination of the quantity of enzyme present as a result of an immunoassay procedure, the enzyme containing component is combined with a substrate which in the presence of the enzyme...
4774177 Immunoassay method for detection of antibodies and antigens  
A method for detecting the presence of antibody or antigen in a liquid sample suspected of containing the antibody or antigen. To a test panel comprising a nitrocellulose carrier having stably...
4770996 Identification of individuals predisposed toward alcohol abuse  
A method and an assay for ascertaining individuals likely to be predisposed to alcohol abuse is disclosed. The contemplated method ascertains the relative adenylate cyclase and monoamine oxidase...
4769321 Assay method and reagent therefor  
Enzymatic assays are described which enable the detection of inter alia enzymes, cofactors and substrates in small quantity with the ability to produce a visually detectable change.
4748115 Substrate formulation in 2-amino-2-methyl-1-propanol buffer for alkaline phosphatase assays  
A stable substrate formulation for an alkaline phosphatase assay includes 5-bromo-4-chloro-3-indolyl phosphate, nitro blue tetrazolium and a 2-amino-2-methyl-1-propanol buffer. Addition of MgCl2 to...