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4879218 Antibody for C.difficile  
Monoclonal antibody to toxin A of C.difficile has been prepared and is used in an assay for toxin A of C.difficile.
4861709 Detection and/or identification of microorganisms in a test sample using bioluminescence or other exogenous genetically-introduced marker  
A method for determining the presence of microorganisms in a tests sample. Exogenous DNA containing a luminescent system or other genetic marker system derived from a suitable donor source is...
4855227 Rapid detection of mycoplasmas  
A clinical diagnostic method capable of rapidly detecting the presence of Mycoplasma pneumoniae in infected humans is taught. The method allows a proper course of therapy to be chosen within one...
4849337 Assaying allergen specific IgE levels with fluorogenic enzyme labeled antibody  
A method for identifying and quantifying allergen specific IgE levels in patent serum by conjugating the IgE in the serum with allergens adhering to an insoluble support, conjugating the serum IgE...
4849332 Human papillomavirus 35 nucleic acid hybridization probes and methods for employing the same  
Nucleic acid hybridization probes for human papillomavirus types and particularly human papillomavirus type 35; and methods for employing the same.
4849334 Human papillomavirus 43 nucleic acid hybridization probes and methods for employing the same  
Nucleic acid hybridization probes for human papillomavirus types and particularly human papillomavirus type 43; and methods for employing the same.
4849335 Method for detecting the mutagenicity of substances susceptible of inducing the deterioration of cellular DNA, utilizing the production of an SOS response  
A method for the detection of the mutagen effect of a substance or composition by producing culture of a micro-organism harboring a recombinant of a sfi gene and of a gene coding for a dosable...
4849331 Human papillomavirus 44 nucleic acid hybridization probes and methods for employing the same  
Nucleic acid hybridization probes for human papillomavirus types and particularly human papillomavirus type 44; and methods for employing the same.
4845027 Fluorometric assay of allergic reactions  
A method for identifying and quantifying allergen specific IgE levels in patient serum by conjugating the serum IgE with allergens on an insoluble support, conjugating the serum IgE with an enzyme...
4844966 Assaying total IgE levels with fluorogenic enzyme labeled antibody  
A method for identifying and quantifying total IgE levels in patient serum by conjugating the IgE in the serum with anti-IgE antibody adhering to an insoluble support, conjugating the serum IgE...
4845028 Composition for lipase assay  
A composition and method for lipase assay, comprising the use of an aqueous solution of a 1,2-diglyceride of a higher fatty acid, and a nonionic surface active agent. The higher fatty acid is a...
4840895 Monoclonal antibodies reactive with immune complexes  
A secondary monoclonal antibody against a complex of a molecule of molecular weight less than 5000 and a binding protein against said molecule which secondary monoclonal antibody is not an antibody...
4835099 Signal enhancement in immunoassay by modulation of enzymatic catalysis  
A method for enzyme immunoassay for a ligand suspected to be present in a liquid sample includes signal amplification by use of at least two hydrolases and a blocked fluoroketone for one of the...
4830960 Determination of chlamydia trachomatis  
A process for determining qualitatively or quantitatively the presence of Chlamydia Trachomatis or Chlamydia trachomatis derived material in a subject material which process comprises deriving a...
4830959 Electrochemical enzymic assay procedures  
Electrochemical detection of mediator level is employed in a method of assay using a redox enzyme and redox substrate to detect conversion to an effective mediator by an assay enzyme label of a...
4828982 Vesticles and use thereof in an enzyme assay  
A sac is produced by use of a compound which includes a hydrophobic portion and a polar group which is hydrolyzable by an enzyme and which is spaced from the hydrophobic portion by a hydrophilic...
4828985 Antibodies against the complex of a small molecule and its binding protein, their preparation and their use in diagnostic methods  
The invention involves polyclonal antibodies reactive with a complex of a hapten and its binding protein, wherein the antibodies are unreactive with the free hapten or free binding protein. The...
4820627 Method of preparing particles suitable for tabletting into diagnostic reagents  
A completely water-soluble, solid, labile biochemical-containing diagnostic reagent is prepared without the need of a lyophilization step, by spraying, preferably by a fluidized bed process, an...
4812396 Method for detecting enzymatic activity using particle agglutination  
A method is disclosed for determining enzymatic activity in a liquid sample by particle agglutination or inhibition of particle agglutination.
4812400 Process for measuring sodium levels in biological fluids  
A process for measuring the sodium concentration of a biological fluid, such as blood serum or urine, is carried out by fixing the amounts of adenosine-5'-triphosphate (ATP), adenosine...
4810631 Signal enhancement in immunoassay by modulation of chemical catalysis  
A method for immunoassay for a ligand suspected to be present in a fluid includes use of an enzyme, a metal ion catalyst for an indicator reaction and a blocked modulator for the catalyst. Ligand...
4810636 Chromogenic acridinone enzyme substrates  
Chromogenic acridinone enzyme substrate compounds comprising 7-hydroxy-9H-acridin-2-one chromogens derivatized at the 7-hydroxy-position with an enzymatically-cleavable group and disubstituted at...
4808522 Enzyme immunoassays using inorganic pyrophosphatase  
A method for determination of an antigen, which comprises preparation of a onjugate by way of cross-linking of an antibody with an inorganic pyrophosphatase, reacting the resulting conjugate with...
4806470 Analytical element and method for theophylline determination having increased alkaline phosphatase isoenzyme  
Theophylline can be determined with an analytical element and method which utilize the inhibition, by theophylline, of alkaline phosphatase activity on an appropriate substrate. The assay is...
4806415 Method and system for determining the presence of adenosine triphosphate or flavin mononucleotide  
An enzymatic determination of one of the co-enzymes adenosine triphosphate (ATP) or flavin mono-nucleotide (FMN) based on the use of an flavine adenine dinucleotide (FAD) synthetase-active system...
4803157 Hydrolyzable fluorescent substrates for phosphatases and analytical use thereof  
Hydrolyzable substrates for acid and alkaline phosphatases comprise blocked dye moieties which, when cleaved from the substrate during hydrolysis, provide fluorescent dyes having maximum...
4795701 Homogeneous polynucleotide displacement assay method kit and reagent complex  
An RNA signal strand is displaced from a probe strand by a target nucleotide sequence. Without separation, a digestion enzyme selective for displaced RNA, e.g., a polynucleotide phosphorylase,...
4788143 Method for determining levels of cell-mediated immunity  
A patient's level of cell-mediated immunity is determined by obtaining a blood sample from the patient, incubating cells from the sample in a tissue culture medium containing Concanavalin A, and...
4782016 Analytical element and method for determination of theophylline by enzyme inhibition  
Theophylline can be determined with an analytical element, composition, kit and method which utilize the inhibition, by theophylline, of alkaline phosphatase activity on an appropriate substrate....
4782017 Analytical element and method for determination of theophylline by enzyme inhibition  
Theophylline can be determined with an analytical element and method which utilize the inhibition, by theophylline, of alkaline phosphatase activity on an appropriate substrate. The assay is...
4778755 Immunoassay method  
In the determination of the quantity of enzyme present as a result of an immunoassay procedure, the enzyme containing component is combined with a substrate which in the presence of the enzyme...
4774177 Immunoassay method for detection of antibodies and antigens  
A method for detecting the presence of antibody or antigen in a liquid sample suspected of containing the antibody or antigen. To a test panel comprising a nitrocellulose carrier having stably...
4770996 Identification of individuals predisposed toward alcohol abuse  
A method and an assay for ascertaining individuals likely to be predisposed to alcohol abuse is disclosed. The contemplated method ascertains the relative adenylate cyclase and monoamine oxidase...
4769321 Assay method and reagent therefor  
Enzymatic assays are described which enable the detection of inter alia enzymes, cofactors and substrates in small quantity with the ability to produce a visually detectable change.
4748115 Substrate formulation in 2-amino-2-methyl-1-propanol buffer for alkaline phosphatase assays  
A stable substrate formulation for an alkaline phosphatase assay includes 5-bromo-4-chloro-3-indolyl phosphate, nitro blue tetrazolium and a 2-amino-2-methyl-1-propanol buffer. Addition of MgCl 2 ...
4735897 Method and kit for detecting polyriboadenosine segments and messenger RNA  
RNA such as messenger RNA is digested to nucleotide phosphates including AMP or ADP. The ATP or a byproduct of the phosphorylation, e.g., pyruvate, is detected. Exemplary enzymes used (with...
4725539 Method for analyzing plural oxidizable components in a liquid  
A new method of rapidly analyzing plural substances in the presence of biological catalyzers is disclosed. The method is practiced by way of the steps of injecting both pH buffer solution and...
4720458 Heat sensitive bacterial alkaline phosphatase  
This invention discloses a newly discovered enzyme and a method of producing said enzyme wherein said enzyme is completely inactivated by treatment with temperatures of 50° or higher for 10...
4719097 Phosphates of resorufin derivatives and compositions thereof for the determination of the activity of phosphatases  
The present invention provides phosphates of resorufin derivatives of the general formulae: ##STR1## wherein R 2 and R 5 , which can be the same or different, are hydrogen or halogen atoms or...
4717652 Composition for detection of peritoneal inflammation or infection  
Peritoneal inflammation or infection can be detected in a patient by assaying a peritoneal lavage sample from the patient for lysosomal enzymes. This is done by combining the lavage sample with a...
4707438 Immunoassay for breast cancer employing monoclonal antibodies  
An immunoassay for diagnosing and monitoring human breast cancer. The assay employs a monoclonal antibody which recognizes a human mammary tumor virus derived from the T47D clone-10 breast cancer...
4690890 Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay  
At least two antigens in a sample may be detected using an immunometric dual sandwich assay containing an effective amount of at least one monoclonal antibody against each antigen, which antibodies...
4687732 Visualization polymers and their application to diagnostic medicine  
A method for detecting a minute quantity of an inorganic or organic target molecule by combining it with a composition of a detecting agent for the target molecule which carries, by direct or...
4681842 Process and reagent for the differentiated determination of isoenzymes of alkaline phosphatase  
The subject of the application is a process and a reagent for the differentiated determination of the bone and liver isoenzyme of alkaline phosphatase in a sample, whereby this is mixed with a...
4681841 Enzymatic assay method  
A method for assaying a compound of the formula ##STR1## wherein R 1 is hydroxyl or amino, or hydrogen if at least one of R 2 , R 3 , R 4 , R 5 and R 6 is hydroxyl or amino, and R 2 , R 3 , R 4...
4670381 Heterogeneous immunoassay utilizing horizontal separation in an analytical element  
A heterogeneous, competitive binding immunoassay is conducted with a dry analytical element. The immunoassay is useful for determining a ligand (e.g. a therapeutic drug) and includes contacting a...
4668623 Method of fluorometrically measuring the activity of fat-degrading enzymes  
The object of the present invention is a new method for fluorometrically assaying the activity of fat-degrading enzymes, such as lipases and phospholipases in samples containing said enzyme, such...
4665019 Method for measuring the plasma levels of an inhibitor of (Na.sup.+ +K.sup. +  
A method for measuring plasma levels of an inhibitor of (Na + +K + )ATPase associated with hypertension comprising: (1) incubating deproteinized plasma of a patient suspected of being...
4661444 Homogeneous immunoassays employing double antibody conjugates comprising anti-idiotype antibody  
Homogeneous immunoassays employing double ligand binding conjugates comprising an anti-idiotype binding partner. The anti-idiotype binding partner competes with ligand for an insolubilized ligand...
4659657 Chromogenic and fluorogenic esters for photometric or fluorimetric determination of phosphatases or sulphatases  
Compounds of the formula ##STR1## wherein Y is ##STR2## M is hydrogen, or an alkali metal or ammonium ion, X is --O-- or an amino group, R is H, Cl, Br, CN or carbamoyl, R 1 is H or --SO 3 ...