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7566555 |
Method of expressing proteins comprising non-naturally-occurring amino acids
A method of expressing a protein having an unnatural amino acid integrated thereinto comprising expressing (A) a mutant tyrosyl tRNA synthase that is derived from Escherichia coli -origin tyrosyl...
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7560248 |
Oxidation-resistant ribonuclease inhibitor
Mutant forms of ribonuclease inhibitor are described which are rendered more resistant to oxidation while retaining affinity for both ribonuclease and angiogenin. The mutant forms have another...
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7544487 |
Fusion proteins containing recombinant cytotoxic RNAses
Recombinant immunotoxins containing a cytotoxic RNAse fused to an antibody or antibody fragment may be produced in mammalian cell culture. Surprisingly, immunotoxins containing a cytotoxic RNAse...
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7527956 |
Modified human acid sphingomyelinase having increased activity, and methods for making the same
Deficiencies in the enzymatic activity of acid sphingomyelinase (ASM) result in Niemann-Pick disease. A variety of modifications which eliminate the activity of the free thiol on the C-terminal...
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7527947 |
Signal peptide for producing a polypeptide
The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal...
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7527943 |
Compositions of orthogonal glutamyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
Compositions and methods of producing components of protein biosynthetic machinery that include glutamyl orthogonal tRNAs, glutamyl orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of...
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7525015 |
Prevention of transgene escape in genetically modified perennials
Genes can be introduced into plants that confer desirable traits such as, drought and stress tolerance, insect and pest resistance, as well as environmental qualities such as phyto-remediation....
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7495148 |
Double recoverable block of function
An improved double Recoverable Block of Function (RBF) system for transgene containment with enhanced reliability is disclosed. The system includes two blocking constructs (BCs) at the opposite...
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7491524 |
Human RNase III and compositions and uses thereof
The present invention provides polynucleotides encoding human RNase III and polypeptides encoded thereby. Methods of using said polynucleotides and polypeptides are also provided.
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7476500 |
In vivo selection system for enzyme activity
The present invention provides in vivo systems in which activity of a biological cleavage enzyme, such as a site-specific recombinase, a homing endonuclease, or an intein, is linked to cell...
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7476391 |
Anti-bacterial vaccine compositions
Gram negative bacterial virulence genes are identified, thereby allowing the identification of novel anti-bacterial agents that target these virulence genes and their products, and the provision of...
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7473542 |
Cysteinized ribonuclease and targeting moiety conjugated thereto
A cysteinized variant of an RNase disclosed in U.S. Pat. No. 6,239,257 B1 is disclosed. A targeting moiety can be conjugated to the cysteine residue.
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7459298 |
Purification, cloning and biochemical characterization of XendoU, endoribonucleasic activity involved in small nuclear RNA splicing-independent biosynthesis in Xenopus laevis
Cloning and characterization of the gene for Mn ++ dependent RNA-sc endoribonucleasic activity able to generate 2′-3′ cyclic phosphate and 5′OH ends.
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7453025 |
Reproductive ablation constructs
The present invention relates to the regulation of reproductive development, particularly to the genetic ablation of reproductive tissues in angiosperm and gymnosperm species....
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7442535 |
Fusion protein incorporating a cysteinized ribonuclease
A fusion protein contains a cysteinized variant of an RNase disclosed in U.S. Pat. No. 6,239,257 B1.
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7439035 |
Method of identifying or characterizing a compound that modulates ribonuclease H activity
The invention relates to a substrate and method for the assay of ribonuclease H activity. The substrate comprises a DNA/RNA heteroduplex molecule having fluorophore and quencher molecules in close...
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7432365 |
DNA molecules encoding beta clamp proteins of gram positive bacteria
The present invention relates to beta protein-encoding genes from Gram positive bacterium. Expression systems and host cells containing these genes are also disclosed.
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7432308 |
Therapeutic compositions for the treatment of a respiratory tract disease
The equilibrium between G actin and F actin is shifted towards the F actin confirmation with compounds such as the ionic form of magnesium or potassium. The higher amount of F actin decreases the...
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7429468 |
Mutant B-type DNA polymerases exhibiting improved performance in PCR
The present invention relates to thermostable mutants of B-type DNA polymerases comprising a Y-GG/A amino acid motif between the N-terminal 3′-5′-exonuclease domain and the C-terminal...
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7422889 |
Dre recombinase and recombinase systems employing Dre recombinase
The invention provides a Dre/rox recombinase system. In particular, the invention provides Dre polypeptides that can catalyze site-specific recombination at rox sites but not at lox sites. The...
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7422888 |
Thermotolerant ribonuclease H
Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.
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7410647 |
Antigenic polypeptides
A method for the identification of antigenic polypeptides, typically opsonic antigens, expressed by pathogenic microbes; vaccines comprising said antigens; and therapeutic antibodies directed to...
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7399476 |
Nucleic acid free ghost preparations
The invention relates to preparations of bacterial ghosts which are substantially free of living bacterial cells and/or nucleic acids and their use in pharmaceutical preparations.
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7378262 |
Reversibly modified thermostable enzymes for DNA synthesis and amplification in vitro
The invention relates to a composition comprising a first modified thermostable enzyme exhibiting 3′exonuclease activity but essentially no DNA polymerase activity and a second modified...
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7247464 |
CspCI restriction endonuclease
A novel restriction endonuclease has been identified from Citrobacter species 2144 (NEB#1398) which can cleave at nt sequence 5′-CAANNNNNGTGG-3′ (SEQ ID NO:13) in double-stranded DNA molecules.
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7238853 |
Molecular mechanisms for gene containment in plants
The present invention provides a molecular mechanism for gene containment in sexually reproducing transgenic plants. The mechanism is achieved with a molecular construct comprising a blocking...
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7232678 |
Identification of oligoadenylate synthetase-like genes
The present invention relates to compositions and methods for modulating conception in animals. More particularly, the composition modulates mRNA degradation during gametogenesis and early...
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7189546 |
Polypeptide, cDNA encoding the same, and use of them
A new polypeptide prepared from human library by the SST method and a process for preparation of it; a cDNA encoding the polypeptide; a fragment selectively hybridizing with the sequence of the...
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7189393 |
Recombinant anti-tumor RNAse
This invention provides for new recombinant ribonuclease proteins which are active when expressed by bacteria. This allows the recombinant ribonucleases of this invention to be fused in-frame with...
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7186538 |
Type II restriction endonuclease, CstMI, obtainable from Corynebacterium striatum M82B and a process for producing the same
In accordance with the present invention, there is provided a novel type II restriction endonuclease, obtainable from Corynebacterium striatum M82B, hereinafter referred to as “CstMI”, which...
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7186520 |
Substrate for assaying ribonuclease H activity
The invention relates to a substrate and method for the assay of ribonuclease H activity. The substrate comprises a DNA/RNA heteroduplex molecule having fluorophore and quencher molecules in close...
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7150982 |
RNA detection assays
The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection...
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7135324 |
Viral recombinases, related articles, and methods of use thereof
A Herpes simplex virus (HSV) recombinase comprises a purified or isolated alkaline nuclease and a single stranded DNA binding protein. In HSV-1, the alkaline nuclease is the UL12 protein and the...
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7129075 |
Isolated CEL II endonuclease
The present invention relates to the isolation and characterization of CEL I and CEL II endonuclease proteins. Methods and kits for identifying mismatches in double-stranded DNA are also provided.
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7122364 |
FEN endonucleases
The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection...
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7118901 |
Recombinant bovine pancreatic desoxyribonuclease I with high specific activity
A method is provided for the production of a bovine pancreatic protein with a specific desoxyribonuclease activity of at least 6,000 units per mg of protein. Methylotrophic yeast is used as a...
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7115407 |
Recombinant type II restriction endonucleases, MmeI and related endonucleases and methods for producing the same
In accordance with the present invention, there is provided a DNA (deoxyribonucleic acid) fragment which encodes the MmeI type II restriction endonuclease enzyme. This one polypeptide possesses two...
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7101697 |
Restriction endonucleases, method of synthesis and use thereof
A restriction endonuclease having one DNA binding site is proposed, synthesized from a restriction endonuclease that has one C-terminal domain and one N-terminal domain and two DNA binding sites,...
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7098016 |
Ribonuclease zymogen design
An enzyme is re-engineered to be a zymogen, an enzyme precursor which is converted into an enzyme by protease cleavage. In the example described here, an RNase A enzyme is converted into a zymogen...
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7094560 |
Stabilization of T4 endonuclease V
The invention provides T4 endonuclease V compositions that exhibit enhanced stability, including stability at non-refrigerated temperatures, through reduced activity of cryptic proteases. Methods...
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7091026 |
Artificial endonuclease
The present invention provides artificial endonucleases and methods to prepare and use those endonucleases.
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7081358 |
Method for engineering strand-specific, sequence-specific, DNA-nicking enzymes
Methods are provided for converting into a sequence specific strand specific and location specific DNA nicking endonuclease, a restriction endonuclease that recognizes an asymmetric DNA sequence,...
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7067298 |
Compositions and methods of using a synthetic Dnase I
Compositions and method for making and using a synthetic bovine DNase I are disclosed. More particularly, the sbDNase I of the present invention is a versatile enzyme that cleaves DNA...
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7067135 |
Methods and compositions for the treatment and prevention of Staphylococcus aureus infections
The invention features methods and compositions for treatment or prevention of infection by, or disease caused by infection with, Staphylococcus spp., particularly S. aureus.
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7052897 |
Alteration of restriction endonuclease specificity by genetic selection
Methods and compositions are provided for altering the DNA recognition and cleavage characteristics of an endonuclease without prior knowledge of the endonuclease's three-dimensional structure...
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7049123 |
Deoxyribonuclease, gene encoding same and use thereof
This invention provides a novel acid DNase (DLAD) which is an endonuclease capable of cleaving DNA independently from divalent cations, under acidic conditions, which retains its activity in acidic...
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7029900 |
Type II restriction endonuclease BtgZI, obtainable from Bacillus thermoglucosidasius 36A and a process for producing the same
In accordance with the present invention, there is provided a novel restriction endonuclease obtainable from Bacillus thermoglucosidasius 36A (NEB#1384), hereinafter referred to as “BtgZI”,...
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7029899 |
Selective toxicity of amino-terminal modified rnase a superfamily polypeptides
This invention provides RNase A superfamily polypeptides with modified amino terminal which can be used to selectively kill target Kaposi's sarcoma cells, neoplastic endothelial cells, and...
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7018825 |
DNase liquid solutions
The present invention relates to the use of calcium ion and/or sugars to minimize thermal aggregation of DNase and to the use of calcium ion to stabilize liquid solutions of DNase, the solutions...
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7011966 |
Method for cloning and expression of AcuI restriction endonuclease and AcuI methylase in E. coli
The present invention relates to recombinant DNA encoding the AcuI restriction endonuclease as well as AcuI methylase, and expression of AcuI restriction endonuclease and AcuI methylase in E. coli...
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